Preparation method of animal extract capable of brightening hair

Abstract

The invention discloses a preparation method of an animal extract capable of brightening hair, which comprises the following steps of: extracting effective components from the liver of a mammal through digestion and enzymolysis, centrifuging to remove supernate, concentrating, frying to obtain dry extract powder, and adding maltose, so that the prepared extract can brighten the hair of people, minks, dogs, pigs and the like, and has the effects of improving the hair quality and the like. According to the invention, the mammal liver is used as a main raw material, the extract is prepared by adopting natural enzymolysis and other processes, and the added maltose is also a natural food material, so that the product has higher safety and naturalness, and is suitable for people and various animals.

Description

Technical Field

[0001] The invention relates to the technical field of preparation of animal extracts, in particular to a method for preparing an animal extract capable of making hair shiny. Background Art

[0002] Hair health is a significant concern in pet breeding (such as minks, dogs, and pigs) and in human beauty and skincare. Numerous products are currently available on the market for improving hair quality, but these products suffer from varying effectiveness, complex ingredients, and the potential presence of harmful chemicals. Therefore, a natural, effective, and safe solution for achieving shiny hair is needed. Mammalian livers contain a variety of nutrients, such as protein, vitamins, and minerals, which may have positive effects on hair health. However, there are currently no reports specifically using mammalian liver to prepare extracts for improving hair quality. Summary of the Invention

[0003] The purpose of the present invention is to provide a method for preparing an animal extract that makes hair shiny. The active ingredients are extracted from the liver of mammals through a specific process and maltose is added. The obtained extract can make the hair of humans, minks, dogs, pigs, etc. shiny, so as to solve the problems raised in the above background technology.

[0004] To achieve the above object, the present invention provides the following technical solutions: The present invention provides a method for preparing an animal extract for making hair shiny, comprising the following steps: S1. Select fresh mammalian liver as raw material, including but not limited to pigs, cattle, sheep, etc. Remove surface impurities such as fascia and blood vessels from the selected liver and clean it thoroughly. Cut the cleaned liver into small pieces, add digestive fluid, and digest at 37-40°C for 4-6 hours with stirring to fully decompose the liver tissue, thereby obtaining a digestive extract. S2. Add papain to the digestion extract and perform enzymolysis at 50-60°C for 5-8 hours. Stir continuously during the enzymolysis process to ensure uniform enzymolysis. After the enzymolysis is completed, centrifuge at 4000 rpm for 20 minutes to obtain the supernatant. S3, concentrating the supernatant under reduced pressure to 1 / 5-1 / 10 of its weight before concentration, and then frying it in a low-temperature vacuum drying apparatus at 50° C. and a vacuum degree of -0.1 MPa to obtain a dry extract powder; S4. Mix the extract powder with malt sugar, stir thoroughly and evenly to obtain the animal extract that makes the hair shiny.

[0005] Furthermore, in step S1, the mass-to-volume ratio of the liver to the digestive fluid is 1:2-1:5.

[0006] Furthermore, the digestive fluid is a phosphate buffer containing 0.1%-0.5% pepsin and 0.05%-0.2% trypsin, with a pH value of 7.0-8.0.

[0007] Furthermore, in step S1, the liver is a liver of a mammal, and the mammal is a pig, a cow, or a sheep.

[0008] Furthermore, in step S2, the amount of papain added is 0.5%-0.8% of the weight of the liver used in step S1.

[0009] Furthermore, in step S4, the mass ratio of maltose to extract powder is 1:1-3:1.

[0010] The present invention also provides a use of the extract prepared by the above preparation method in products for shiny hair and firm skin of animals, including humans, minks, dogs, pigs, etc.

[0011] Compared with the prior art, the present invention has the following beneficial effects: The extract prepared by the present invention contains a variety of nutrients extracted from mammalian liver, such as amino acids, vitamin A, lecithin, etc. These ingredients can nourish hair, provide necessary nutrition for hair, promote hair growth and health, and make hair shiny and smooth.

[0012] The collagen, elastin and other ingredients in the extract of the present invention help increase skin elasticity, reduce the formation of skin wrinkles, and have a skin-tightening effect. At the same time, these nutrients can also improve blood circulation in the skin, enhance skin metabolism, and further promote skin health.

[0013] The present invention uses mammalian liver as the main raw material and adopts natural enzymatic hydrolysis and other processes to prepare the extract. The added maltose is also a natural ingredient. Compared with some products on the market containing chemically synthesized ingredients, the product of the present invention has higher safety and naturalness and is suitable for use by humans and various animals. DETAILED DESCRIPTION

[0014] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0015] A method for preparing an animal extract for making hair shiny comprises the following steps: Raw material preparation Fresh mammal livers are selected as raw materials, including but not limited to pigs, cattle, sheep, etc. The selected livers are cleaned and then removed of impurities such as fascia and blood vessels on the surface, and then used.

[0016] Digestion Cut the cleaned liver into small pieces and add digestive solution at a ratio of 1:2-1:5 liver weight to digestive solution volume. The digestive solution consists of a phosphate buffer solution with a pH of 7.0-8.0, containing 0.1%-0.5% pepsin and 0.05%-0.2% trypsin. Digest at 37-40°C with stirring for 4-6 hours to fully decompose the liver tissue.

[0017] Enzymatic hydrolysis reaction After digestion, add 0.5%-0.8% of the liver weight of papain to the digestion extract and perform enzymatic hydrolysis at 50-60°C for 5-8 hours. Stir constantly during the enzymatic hydrolysis to ensure uniform enzymatic hydrolysis.

[0018] Filtration and concentration After the enzymatic hydrolysis is completed, centrifuge at 4000 rpm for 20 minutes to collect the supernatant. The supernatant is then concentrated under reduced pressure at 50°C and -0.1 MPa to 1 / 5-1 / 10 of its original weight.

[0019] Stir-frying The concentrated liquid was placed in a low-temperature vacuum drying device and fried to dryness under the conditions of a temperature of 50° C. and a vacuum degree of -0.1 MPa to obtain a dry extract powder.

[0020] Add malt sugar The maltose is added to the extract powder in a weight ratio of 1:1 to 3:1, and the mixture is fully stirred to obtain the shiny hair animal extract of the present invention. Example 1

[0021] S1. Prepare 1000 g of fresh pig liver, remove surface impurities such as fascia and blood vessels, and rinse three times with clean water. Cut the cleaned liver into small pieces approximately 1 cm³. Add 2000 ml of pH 7.0 phosphate buffer containing 0.1% pepsin and 0.05% trypsin. Digest with stirring at 37°C for 4 hours.

[0022] S2. After digestion, add 5 g of papain to the digestion solution and perform enzymatic hydrolysis at 50°C for 5 hours with constant stirring.

[0023] S3. After the enzymatic hydrolysis is completed, centrifuge at 4000 rpm for 20 minutes and take the supernatant; then concentrate the supernatant under reduced pressure at a temperature of 50°C and a pressure of -0.1 MPa to 1 / 5 of its weight before concentration to obtain 600 g of concentrated solution; place the concentrated solution in a low-temperature vacuum drying equipment and fry it to dryness at a temperature of 50°C and a vacuum degree of -0.1 MPa to obtain 30.5 g of dry extract powder.

[0024] S4. Take 30.5 grams of maltose and add it to the extract powder, stir thoroughly to obtain 61 grams of hairy shiny animal extract. Example 2

[0025] S1. Select 1000 g of fresh pig liver, remove impurities and clean it as in Example 1, cut it into small pieces, add 3500 ml of phosphate buffer with a pH value of 7.5, containing 0.3% pepsin and 0.12% trypsin, and stir and digest at 38°C for 5 hours.

[0026] S2. After digestion, add 6.5 g of papain to the digestion solution and perform enzymatic hydrolysis at 55°C for 6.5 hours with constant stirring.

[0027] S3. After the enzymatic hydrolysis is completed, the supernatant is taken by centrifugation at 4000 rpm for 20 minutes, and then the supernatant is concentrated to 1 / 8 of the weight before concentration at a temperature of 50°C and a pressure of -0.1 MPa to obtain 560 g of concentrated solution; the concentrated solution is placed in a low-temperature vacuum drying equipment and fried to dryness at a temperature of 50°C and a vacuum degree of -0.1 MPa to obtain 33 g of dry extract powder.

[0028] S4. Take 66 grams of maltose and add it to the extract powder, stir thoroughly to obtain 99 grams of hairy shiny animal extract. Example 3

[0029] S1. Select 1000 g of fresh pig liver, remove impurities and clean it as in Example 1, cut it into small pieces, add 5000 ml of phosphate buffer with a pH value of 8.0, containing 0.5% pepsin and 0.2% trypsin, and stir and digest at 40°C for 6 hours.

[0030] S2. After digestion, add 8 g of papain to the digestion solution and perform enzymatic hydrolysis at 60°C for 8 hours with constant stirring.

[0031] S3. After the enzymatic hydrolysis is completed, centrifuge at 4000 rpm for 20 minutes to obtain the supernatant, and then concentrate the supernatant to 1 / 10 of its weight before concentration at a temperature of 50°C and a pressure of -0.1 MPa to obtain 600 g of concentrate; the concentrate is placed in a low-temperature vacuum drying equipment and fried to dryness at a temperature of 50°C and a vacuum degree of -0.1 MPa to obtain 31.5 g of dry extract powder.

[0032] S4. Take 94.5 grams of maltose and add it to the extract powder, stir thoroughly to obtain 126 grams of hairy shiny animal extract. Test Example 1

[0033] Twenty adult dogs with normal hair and skin were randomly divided into four groups of 20 each. The experimental groups were fed 5 grams of the extracts prepared in Examples 1-3 daily, while the control group was not fed. After three months of continuous feeding, the experimental groups showed significantly shinier and smoother hair, firmer skin, and less hair loss than the control group. Test Example 2

[0034] Twenty volunteers with dry hair and rough skin were recruited, half male and half female. The volunteers were randomly divided into four groups of five. The experimental group applied 10 grams of the extract prepared in Examples 1-3 to their hair and skin daily, gently massaging until absorbed, for six months. The results showed that the experimental group experienced significantly improved hair gloss, enhanced skin elasticity, and improved dryness and roughness.

[0035] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0036] It should be noted that the above content merely illustrates the technical idea of the present invention and cannot be used to limit the scope of protection of the present invention. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications all fall within the scope of protection of the claims of the present invention.

Claims

1. A method for preparing an animal extract for making hair shiny, characterized in that: The following steps are involved: S1. Cut the cleaned mammalian liver into small pieces, add digestive fluid, and digest with stirring at 37-40° C. for 4-6 hours to obtain a digestive extract; S2. Add papain to the digestion extract and perform enzymatic hydrolysis at 50-60°C for 5-8 hours. Centrifuge and collect the supernatant. S3, concentrating the supernatant under reduced pressure to 1 / 5-1 / 10 of its weight before concentration, and then frying to dryness at 50° C. and a vacuum degree of -0.1 MPa to obtain a dry extract powder; S4. The extract powder is mixed with maltose to obtain an animal extract that makes hair shiny.

2. The method for preparing an animal extract for making hair shiny according to claim 1, characterized in that: In step S1, the mass-to-volume ratio of the liver to the digestive fluid is 1:2-1:

5.

3. The method for preparing an animal extract for making hair shiny according to claim 2, characterized in that: The digestion solution is a phosphate buffer containing 0.1%-0.5% pepsin and 0.05%-0.2% trypsin, with a pH value of 7.0-8.

0.

4. The method for preparing an animal extract for making hair shiny according to claim 1, characterized in that: In step S1, the liver is a liver of a mammal, and the mammal is a pig, a cow or a sheep.

5. The method for preparing an animal extract for making hair shiny according to claim 1, characterized in that: In step S2, the amount of papain added is 0.5%-0.8% of the weight of the liver used in step S1.

6. The method for preparing an animal extract for making hair shiny according to claim 1, characterized in that: In step S4, the mass ratio of maltose to extract powder is 1:1-3:

1.

7. Use of the extract prepared by the preparation method according to any one of claims 1 to 6 in animal hair shiny products.

8. The use of an extract according to claim 7 in animal hair shiny products, characterized in that: The animals include humans, minks, dogs, and pigs.