Dust-free environment purification method and method for biopharmacy

Abstract

The invention discloses a dust-free environment purification method for biological pharmacy. Comprising the following steps: constructing three layers of metal filter screens, realizing particle interception through a conical micro-column array, an Al2O3 / TiO2 composite ceramic layer and a diamond-like carbon film, fixing lysozyme by adopting a crystal face regulated ZIF-8 crystal, and realizing dual inactivation of microorganisms by combining dielectric barrier discharge of a spiral plasma channel. The electrostatic adsorption HEPA filter paper adopts a BaTiO3-graphene quantum dot composite electret substrate, and a TiO2 / graphene heterojunction catalytic layer is decomposed by a Pt-Co bimetallic nanocluster and a photocatalysis-plasma-ozone-thermocatalysis quadruple oxidation system; and 0.1 mu m organic aerosol triggers graded reverse pulse cleaning based on differential pressure integration and a particle concentration threshold value, and the honeycomb flow guide structure drives a shutter and a gradient density sintering metal flow guide plate through piezoelectric ceramics to maintain laminar flow uniformity. According to the scheme, the limitation of a traditional technology is broken through, the purification efficiency of 0.1 micron particles is improved, and a high-stability solution is provided for a biopharmaceutical clean room.

Description

Technical Field

[0001] The present invention relates to the technical field of biopharmaceutical production environment control, in particular to a dust-free environment purification method for biopharmaceuticals. Background Art

[0002] As the biopharmaceutical industry's requirements for sterile production environments become increasingly stringent, traditional purification technologies face significant challenges in terms of ultrafine particle interception efficiency, dual microbial inactivation mechanisms, and dynamic control capabilities. In particular, in the production of high-end biologics such as gene therapy and monoclonal antibodies, residual nanoscale organic aerosols, secondary microbial growth, and fluctuating environmental parameters under complex operating conditions have become core bottlenecks restricting product quality.

[0003] In existing technologies, traditional three-stage filtration relies on a physical interception mechanism, with an interception efficiency of less than 90% for 0.1-1μm particles, and is unable to effectively decompose microbial metabolites. Oily aerosols are easily adhered to the surface of the metal filter, resulting in a sharp increase in pressure differential and frequent maintenance shutdowns. Microbial inactivation technology mostly uses a single inactivation method of ultraviolet or ozone, with an inactivation rate of only 90%-99% for spore-forming microorganisms, and lacks enzymatic hydrolysis-plasma synergy. Traditional lysozyme immobilization technology has an activity retention rate of less than 60% due to insufficient carrier specific surface area.

[0004] Existing laminar air supply systems rely on fixed wind speeds and guide structures, and are unable to dynamically adjust airflow organization based on pressure differential fluctuations, temperature, and humidity changes, resulting in local turbulence and particle resuspension. Existing TiO2 photocatalytic mineralization rates for volatile organic compounds (VOCs) are only 60%-80%, and the catalyst is inactivated within 200 hours due to carbon deposition and aggregation. The charge half-life of existing electret HEPA filter paper is less than 1,000 hours, and the electrostatic adsorption efficiency decays by >30% under high humidity, resulting in a maintenance cycle deviation of ±20%. Summary of the Invention

[0005] In order to solve the technical problems mentioned in the current background technology, the purpose of the present invention is to provide a dust-free environment purification method and method for biopharmaceuticals.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A dust-free environment purification method for biopharmaceuticals, comprising:

[0008] S1. Arrange multi-stage filtration system in sequence along the airflow direction.

[0009] The first stage is equipped with three layers of metal filter screens, on the surface of which a conical micro-pillar array, an Al2O3 / TiO2 composite ceramic layer and a diamond-like carbon film are respectively provided to intercept suspended particles larger than 1 μm.

[0010] The second stage uses ZIF-8 metal organic framework material with crystal surface regulation, and the surface is cross-linked with glutaraldehyde to fix lysozyme, combined with the dielectric barrier discharge of the spiral plasma channel to inactivate microorganisms.

[0011] The third level sets up electrostatic adsorption HEPA filter paper to capture 0.1-1μm particles. Its electret substrate contains 15% BaTiO3 nanoparticles and 5% graphene quantum dots.

[0012] The fourth stage uses a TiO2 / graphene heterostructured nanocatalytic layer to decompose organic aerosols <0.1 μm through Pt-Co bimetallic nanoclusters and a quadruple dynamic oxidation system;

[0013] S2. Multi-parameter coordinated monitoring and adjustment: deploy a differential pressure transmitter and a particle counter at the three-layer metal filter, and combine it with an ATP bioluminescence meter and a VOCs spectrum sensor to obtain real-time data on pollutants in the environment;

[0014] S3, laminar flow control and dynamic compensation: a honeycomb air outlet is set at the end of the multi-stage filtration system, and a gradient guide plate inclination is used to form a uniform laminar flow. A porosity sintered metal guide plate is arranged on the ground, and the height is dynamically adjusted in combination with a piezoelectric ceramic driver to achieve intelligent adjustment of the direction and speed of the airflow, avoiding secondary contamination of the already purified area.

[0015] S4, pulse cleaning and catalytic regeneration: When the differential pressure integral in the three-stage filter reaches 300 Pa·s, a graded reverse pulse purge is initiated to purge the filter, and ultraviolet pulses are simultaneously activated to decompose carbon deposits. During cleaning, the main and standby fans are coordinated and controlled, and the total air volume balance is maintained through a PID algorithm. The purged particles pass through the spiral plasma channel for secondary treatment.

[0016] Furthermore, the three-layer metal filter is made of 316L stainless steel through a photolithography etching process. The three-layer metal filter includes 5μm, 3μm and 1μm. Different surface treatments are performed on each layer of the filter. The 5μm filter is prepared with a conical micro-pillar array with a height of 3μm and a cone angle of 60° on the surface. The 3μm filter is formed by micro-arc oxidation to form an Al2O3 / TiO2 composite ceramic layer. The 1μm filter is deposited with a diamond-like carbon film by magnetron sputtering.

[0017] The optimal spacing between the three-layer metal filter screens is determined through computer simulation prediction, laboratory measurement verification and actual scene fine-tuning.

[0018] Furthermore, the ZIF-8 metal organic framework material adopts a solvothermal method to grow ZIF-8 crystals on an a-Al2O3 substrate, and through crystal face control, a cubic structure with a {100} crystal face ratio of more than 85% is obtained, which is used to intercept microorganisms with a diameter greater than 3.43.

[0019] The surface of the ZIF-8 metal organic framework material was constructed with an amino surface using 3-aminopropyltriethoxysilane, and lysozyme was immobilized by glutaraldehyde cross-linking. The cross-linker concentration followed the optimal lysozyme activity equation:

[0020] A=0.82[G] 2 -1.75[G]+0.95(0.2%≤[G]≤1.0%)

[0021] When the optimal concentration [G] is determined to be 0.5%, the lysozyme activity retention rate is greater than 92%. The intercepted microorganisms are enzymatically hydrolyzed by lysozyme.

[0022] The spiral plasma channel adopts a double-helix coaxial structure to meet the residence time required for dielectric barrier discharge.

[0023] High-voltage electrodes and ground electrodes are installed at the inlet, middle and outlet of the inner and outer spirals of the spiral plasma channel, respectively. A tangential swirler is set at the air inlet to form a turbulent flow with a Reynolds number Re>4500. The turbulent flow velocity is controlled by a three-section variable frequency centrifugal fan.

[0024] Under the action of high-voltage electrodes, the dielectric barrier discharge causes the gas between the electrodes to be broken down and ionized, causing electrons to accelerate in the strong electric field and collide with neutral molecules to produce an avalanche effect, forming a high-density electron group. The electron avalanche effect decomposes H2O / O2 to generate free radicals, ozone and other active particles. Electrons with energy ≥5eV in the electron group are high-energy electrons. When high-energy electrons collide with gas molecules, the excited state particles de-excite and release ultraviolet photons to form ultraviolet radiation.

[0025] The microorganisms are inactivated by directly bombarding the cell membrane with high-energy electrons to penetrate the peptidoglycan layer and form nanoscale holes in the cell membrane. The ultraviolet radiation induces DNA chain breakage and RNA hydrolysis. The active particles attack the -SH / -OH groups in the active center of the enzyme and the secondary structure of the protein, thereby inactivating the protein and thus causing the inactivation of the microorganisms.

[0026] Decomposition of ZIF-8 enzymatic hydrolysis products: The high-energy electron bombardment directly breaks the CC and CN bonds in the enzymatic hydrolysis products, generates OH hydroxyl radicals and O3 ozone, and gradually oxidizes the macromolecular fragments into CO2 and H2O. Through the turbulent effect, the enzymatic hydrolysis products undergo multiple plasma actions to ensure complete decomposition.

[0027] Furthermore, the electret substrate is constructed through the precise design of polypropylene / barium titanate (BaTiO3) composite fibers, combined with gamma-ray irradiation and bipolar polarization processes to construct a high-stability electret substrate. 15% of BaTiO3 nanoparticles in the composite fibers increase the dielectric constant to 8.5, and 5% of graphene quantum dots (GQDs) form a conductive network to inhibit charge leakage. After 120°C thermal polarization and -20kV corona polarization, an electret electrostatic field is formed, and a 50nm perfluoropolyether (PFPE) coating is applied to the surface of the electret substrate.

[0028] The electret electrostatic field forms a multi-physical field synergy mechanism by integrating the alternating electrode array and the piezoelectric ceramic array.

[0029] 1) The electrostatic field exerts Coulomb force on the charged aerosol.

[0030] 2) Applying a voltage of a specific frequency through an alternating electrode array to form a non-uniform alternating electric field to induce polarization migration of neutral particles, and realizing positive / negative DEP dynamic capture through the non-uniform electric field gradient to fill the electrostatic field blind area.

[0031] 3) The piezoelectric ceramic array drives the aluminum honeycomb substrate at a frequency of 50 to 200 Hz, generating a mechanical wave with an amplitude of 5 to 20 μm, which directionally peels off the particles adsorbed on the surface of the electret substrate.

[0032] Furthermore, the nanocatalytic layer achieves the degradation of microorganisms, organic aerosols and volatile organic compounds through the synergy of functional catalytic design, mass transfer enhancement and dynamic oxidation strategy, which is divided into the following three steps:

[0033] 1) Based on the metal-organic framework confinement effect, a ZIF-8-derived carbon layer was constructed on the titanium dioxide surface to form a molecular sieve structure, precisely anchoring platinum-cobalt bimetallic nanoclusters. The platinum-cobalt bimetallic nanoclusters acted as the active center of the catalyst, preferentially adsorbing organic aerosols with a molecular weight of 200-500 Da. The local reactant concentration was increased through the confinement enrichment effect, and the surface oxidation rate of titanium dioxide reached 0.5 min- 1 At the same time, the sulfonic acid groups modified at the edge of the carbon layer trigger a chain reaction of hydroxyl radicals, achieving the step-by-step breakage of long-chain alkanes and improving the mineralization rate of the organic aerosol. The mineralization refers to the process of completely decomposing organic pollutants into inorganic small molecules through oxidation reactions.

[0034] 2) Using a hydrophobic-hydrophilic gradient carrier, a polydimethylsiloxane hydrophobic layer is coated on the surface of the three-dimensional graphene foam, and carboxyl hydrophilic groups are grafted onto the inner walls of pores with a pore size of 2-50 nm. The hydrophobic region captures non-polar organic aerosols, and the hydrophilic region adsorbs polar components, greatly improving the capture efficiency. Combined with the Archimedean spiral flow channel design, a 10 m / s tangential airflow is applied, and submicron aerosols are enriched on the surface of the catalyst through centrifugal force, extending the residence time of the submicron aerosols to 8 seconds, thereby improving the mineralization rate.

[0035] 3) Establish a four-fold dynamic oxidation system of photocatalysis, plasma, ozone, and thermal catalysis. Ultraviolet light excites TiO2 to generate OH and superoxide radicals, degrading 90% of formaldehyde within 5 minutes. When polycyclic aromatic hydrocarbons are detected, dielectric barrier discharge is activated to generate high-energy electrons, cleaving CC bonds, resulting in a 99% degradation rate of benzopyrene. Ozone is injected under high humidity to oxidize microbial envelope lipids, achieving a virus inactivation rate of >99.99%. Thermal catalysis is activated at 80°C in a dark environment or at night, where the Fe2O3 / ZnO heterojunction activates oxygen to maintain basic oxidation capacity.

[0036] To address the deactivation of the catalyst, an in-situ regeneration strategy was introduced, injecting 0.1% H2O2 steam every 12 hours to remove carbon deposits, and synchronous 40kHz piezoelectric ceramic micro-vibration to prevent nanoparticle agglomeration, thereby extending the catalyst life from 200 hours to 2000 hours.

[0037] Furthermore, the multi-parameter coordinated monitoring and regulation is achieved by deploying embedded differential pressure transmitters before and after each layer of the three-layer metal filter. The measuring range is divided into levels by layer. The embedded differential pressure transmitter is used to measure the pressure difference between the front and rear ends of each level of filter. The pressure difference between the front and rear ends of each level of filter is a pressure differential signal. The Poiseuille equation is used to reversely infer the change in the effective pore ratio of the filter participating in particle interception, thereby accurately evaluating the degree of filter blockage.

[0038] A high-sensitivity piezoelectric resonant particle counter is installed behind each level of the three-layer metal filter to detect suspended particulate matter with a diameter of 0.5-20μm. The sampling frequency is 1Hz. It is linked to the turbulence enhancement structure in S1. When a sudden increase in particles >5μm is detected, the filter vibration frequency is triggered to increase to 120Hz. The high-frequency mechanical vibration removes large particle pollutants accumulated on the filter surface, preventing filter clogging and maintaining the system purification efficiency.

[0039] An ATP bioluminescence detector is embedded in the ZIF-8 layer outlet and in front of and behind the HEPA filter paper. The ATP bioluminescence detector is used to detect the activity of microorganisms in the current environment. The synergistic effect of lysozyme decomposition and plasma inactivation is reflected by the microbial activity. When the microbial activity is greater than 10 3 CFU / m 3 Automatically extend the discharge time to inactivate microorganisms.

[0040] Dual-channel temperature and humidity sensors are placed before and after the HEPA layer to detect the temperature and humidity in the current environment. A compensation model is established based on the humidity sensitivity of the electret material.

[0041] Integrate VOCs spectrum sensor in the nanocatalytic oxidation layer to detect the concentration of 0.1-100ppm of benzene / formaldehyde, etc., and trigger the graded adjustment of photocatalytic intensity.

[0042] Furthermore, the honeycomb air outlet is made of a high-density aluminum alloy to form a honeycomb structure, and the air outlet of the honeycomb structure is integrated with a wind speed sensor, which is used to detect the wind speed of the air outlet.

[0043] The honeycomb structure is provided with piezoelectric ceramic driven guide plates at the inlet, middle and outlet. The surface of the honeycomb structure is anodized to form a 5 μm thick Al2O3 insulating layer. The optimal piezoelectric ceramic driven guide plate inclination angle is determined by computational fluid dynamics simulation to achieve the convergence of the airflow diffusion angle from 45° to 5°, optimize the uniformity of laminar flow and suppress turbulence.

[0044] The main and standby air units are added to the air outlet of the honeycomb. The main fan is an EC backward centrifugal fan, and the standby fan is installed in parallel with the same model fan. The normal state is dormant, and the magnetic coupling coupling is used to achieve impact-free switching within 0.1s.

[0045] Three layers of adjustable guide components are integrated inside the honeycomb. The upper layer uses 316L stainless steel wire mesh to achieve primary rectification, the middle layer uses piezoelectric ceramic driven shutters to dynamically compensate for turbulence, and the lower layer uses 3D printed nylon guide cones. The Indonesian dragon guide cones shape the final flow pattern of the airflow passing through the upper stainless steel wire mesh and the middle piezoelectric shutters to eliminate local eddies.

[0046] The ground guide plate is installed on the ground to correspond to the air outlet of the honeycomb. The ground guide plate adopts gradient density sintered metal porous plate to achieve precise control and dynamic adaptability of air flow distribution.

[0047] A particle counter and a linear piezoelectric ceramic driver are installed on the ground guide plate, and the height of the ground guide plate is dynamically adjusted according to the particle counter data.

[0048] A periodic release of 0.1 MPa compressed air pulses and an ultraviolet catalytic coating are integrated at the bottom of the ground-mounted ground guide plate. The periodic release of 0.1 MPa compressed air pulses removes particulate matter attached to the ground guide plate, and the ultraviolet catalytic coating oxidizes and decomposes organic pollutants attached to the surface of the guide plate into CO2 and H2O.

[0049] Furthermore, the reverse pulse cleaning adopts a pressure difference-particle concentration dual threshold judgment strategy, combining the real-time data of the differential pressure transmitter and the data of the particle counter to dynamically adjust the pulse frequency according to the degree of blockage.

[0050] Differentiated pulse parameters are designed according to the characteristics of different filter layers. The 5μm metal filter is impacted by 0.6MPa reverse 60° airflow for 80ms, and the turbulence effect of the conical micro-pillar array is used to shake off large particles. The ZIF-8 layer is subjected to a 0.3MPa tangential 45° pulse for 120ms, and the cyclonic field of the spiral plasma channel assists in removing bioaerosol residues. The HEPA filter paper is subjected to a 0.2MPa normal 90° pulse for 200ms, combined with the attenuation characteristics of the electret electrostatic field to directionally remove submicron particles. The pulsed airflow is supplied by dual redundant gas tanks.

[0051] The pressure differential integral is the total amount of pressure differential accumulated over time on both sides of each level of filter screen, and is calculated using the following formula:

[0052] ΔP total =∫0 t ΔP(t)dt

[0053] Among them, P(t) is the real-time measured pressure difference before and after each level of filter, t is time (unit: second),

[0054] The reverse pulse cleaning also includes setting a bypass pipe at the cleaning outlet of the three-stage metal filter, ZIF-8 layer and HEPA filter paper, isolating the peeling particles from the main airflow through a three-way valve, installing a negative pressure air pump at the starting point of the bypass pipe, and transporting the particles through the bypass pipe to the spiral plasma channel for secondary processing. The normal air inlet of the spiral plasma channel is closed during cleaning.

[0055] The catalytic regeneration is achieved by starting wavelength-intensity coupled ultraviolet activation after pulse cleaning. Ultraviolet C (UVC) irradiates the nanocatalytic layer to stimulate hydroxyl radicals to decompose organic matter. Long-wave dark spot effect ultraviolet (UVA) assists in activating the lysozyme activity on the ZIF-8 surface. A pulse mode of 5 seconds irradiation / 2 seconds interval is used, and an infrared heating module is used to heat the catalytic layer to 120°C to accelerate the oxidation of carbon deposits. At the same time, 0.5-1ppm ozone is injected to ensure complete decomposition of by-products through the extended path of the spiral plasma channel. The extended path refers to the increased flow distance of the airflow in the plasma channel of the double-helix coaxial structure due to the spiral trajectory.

[0056] During cleaning, the main and standby fans are controlled in coordination. When the pulse airflow is triggered, the main fan speed is reduced to 60% of the rated speed, and the standby fan speed is increased to 80% simultaneously.

[0057] The standby fan outlet is integrated with a pulse buffer chamber, a built-in Helmholtz resonator to absorb pressure pulsations, and a linkage with the ground guide plate. When the pulse pressure is greater than 0.8 MPa, the guide plate switches to turbulence suppression mode.

[0058] If the main fan fails completely, the backup fan switches to 120% overclocking mode for less than 3 minutes, and at the same time activates the electrostatic adsorption enhancement mode of the HEPA filter paper in S1 to compensate for the decrease in filtration efficiency caused by the increase in wind speed.

[0059] The pulse airflow parameters strictly match the mechanical strength and surface characteristics of each level of filter material.

[0060] During cleaning, the height of the ground guide plate is automatically adjusted to maintain the uniformity of air speed in the space at 0.45m / s±0.02m / s.

[0061] Compared with the prior art, the advantages of the present invention are:

[0062] 1. The present invention breaks through the limitations of traditional physical interception. Through the coordinated design of stepped metal filter and composite electret material, combined with surface functionalization treatment and self-cleaning mechanism, it significantly improves the interception efficiency of ultrafine particles, while greatly reducing the risk of filter clogging, extending the stable operation cycle, integrating enzymatic reaction and high-energy plasma action, breaking through the limitations of single inactivation technology, effectively destroying the structure of microorganisms and thoroughly decomposing metabolic products, significantly improving the thoroughness of inactivation and biosafety.

[0063] 2. The present invention achieves efficient mineralization of organic pollutants through the synergistic effects of light excitation, plasma cracking, ozone oxidation and thermal activation by constructing a multi-mode catalytic oxidation network, overcoming the activity decay problem of traditional photocatalytic technology. Based on the feedback control of real-time pressure difference and particle load, it triggers graded pulse cleaning and in-situ regeneration of the catalytic layer, optimizes maintenance triggering logic, reduces unnecessary downtime, and ensures continuous and efficient operation of the system.

[0064] 3. The present invention further suppresses local turbulence and particle resuspension by linking a multi-stage flow-guiding structure with real-time environmental parameters, maintaining the uniformity and stability of the airflow distribution in the clean space. Through material innovation and process optimization, it reduces the performance degradation rate of key components, reduces energy consumption and maintenance costs, and achieves significant optimization of the overall efficiency of the entire life cycle. BRIEF DESCRIPTION OF THE DRAWINGS

[0065] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0066] Figure 1 Schematic diagram of the process of the dust-free environment purification method of the present invention;

[0067] Figure 2 is a schematic diagram of a three-stage filter screen of the present invention;

[0068] Figure 3 Schematic diagram of the ZIF-8 metal organic framework material and the spiral plasma channel of the present invention. DETAILED DESCRIPTION

[0069] To achieve the above objectives, the present invention is implemented through the following technical solutions. The present invention provides a dust-free environment purification method for biopharmaceuticals, the method comprising:

[0070] S1. Arrange multi-stage filtration system in sequence along the airflow direction.

[0071] A three-layer metal filter is made of 316L stainless steel through a photolithography etching process. Different surface treatments are applied to each layer of the filter. The 5μm filter improves the interception of large particles by preparing a conical micro-pillar array with a height of 3μm and a cone angle of 60° on the surface, which enhances the turbulence effect. The 3μm filter uses micro-arc oxidation to generate a 500nm thick Al2O3 / TiO2 composite ceramic layer with a surface potential of +15mV, which reduces particle deposition and clogging through electrostatic repulsion. The 1μm filter uses magnetron sputtering to deposit a 50nm thick diamond-like carbon film, reducing the friction coefficient to 0.08, achieving a self-cleaning function.

[0072] The self-cleaning function is achieved through the triple mechanism of diamond-like carbon film.

[0073] 1) The friction coefficient of diamond-like carbon film is only 0.08, forming a smooth barrier that makes it difficult for particles to adhere.

[0074] 2) The surface contact angle of the diamond-like carbon film reaches 112°, and the water droplets form a rolling effect, carrying away the attached particles.

[0075] 3) The diamond-like carbon film has a surface potential of -45mV and repels charged pollutants through Coulomb force.

[0076] A multi-stage filtration dynamics model was established, and the optimal spacing between each level of the three-layer metal filter was determined by solving the particle motion equation:

[0077]

[0078] Among them, m p is the particle mass, μ is the aerodynamic viscosity, d p Particle diameter, C c Slip correction factor, v g Air velocity, v pParticle velocity, q particle charge, E external electric field strength, ρ p Particle density, g acceleration due to gravity,

[0079] Embedded differential pressure transmitters are deployed before and after the three layers of metal filter screens (5μm, 3μm, and 1μm). The measuring range is divided into different levels (0-50Pa for the 5μm layer, 0-100Pa for the 3μm layer, and 0-150Pa for the 1μm layer), with an accuracy of ±0.05% FS. The Poiseuille equation is used to infer the change in the effective pore ratio of the filter screen involved in particle interception:

[0080]

[0081] Among them, r is the equivalent flow channel radius of the filter, L is the filter thickness, dynamically evaluates the degree of blockage, ΔP is the pressure difference signal, the pressure difference on both sides of the filter, μ is the air dynamic viscosity,

[0082] ZIF-8 crystals were grown on an a-Al2O3 substrate using a solvothermal method. Through crystal face manipulation, a cubic structure with a {100} crystal face ratio of >85% was obtained, which was used to intercept microorganisms with a diameter greater than 3.43. Its window aperture met the following criteria, thereby accurately evaluating the degree of filter clogging:

[0083]

[0084] Among them, a lattice is the lattice constant, r framework is the molecular radius of the ligand methylimidazole,

[0085] 3-Aminopropyltriethoxysilane was used to construct an amino surface with a grafting density of 8.2×10 3 groups / cm3, the enzyme molecules were fixed by glutaraldehyde cross-linking method, and the cross-linker concentration followed the optimal activity equation:

[0086] A=0.82[G] 2 -1.75[G]+0.95(0.2%≤[G]≤1.0%)

[0087] When the optimal concentration [G] is determined to be 0.5%, the lysozyme activity retention rate is greater than 92%. The intercepted microorganisms are enzymatically hydrolyzed by lysozyme.

[0088] The spiral plasma channel adopts a double-helix coaxial structure, with an outer spiral diameter of Φ50mm, an inner spiral diameter of Φ30mm, a pitch of 20mm, a total length of 3.5m, and a spiral angle of 55°, which meets the 2.8ms residence time required for dielectric barrier discharge. Ground electrodes are installed at the entrance, middle and exit of the outer spiral of the spiral plasma channel, and high-voltage electrodes are installed at the entrance, middle and exit of the inner spiral of the spiral plasma channel.

[0089] The high-voltage electrode is made of 316L stainless steel wire woven into a flexible electrode belt with a mesh density of 120 mesh / inch. The grounding electrode is made of aluminum-plated polyimide film with a surface roughness of Ra ≤ 0.8μm. The electrode spacing gradient is designed as 3mm in the inlet section → 2mm in the middle section → 1.5mm in the outlet section. A tangential cyclone is set at the air inlet to form turbulent flow with a Reynolds number Re>4500. The turbulent flow speed is controlled by a three-section variable frequency centrifugal fan in stages, with a speed of 0.8m / s in the front section → 1.2m / s in the middle section → 0.6m / s in the end section.

[0090] Through non-equilibrium gas discharge technology (dielectric barrier discharge) under the action of high voltage electrodes (10-12kV, 10kHz AC), the gas between the electrodes (such as air / oxygen) is broken down and ionized, causing electrons to accelerate in the strong electric field and collide with neutral molecules (such as N2, O2) to produce an avalanche effect, forming a high-density electron group. The electron avalanche effect decomposes H2O / O2 to generate free radicals, ozone and other active particles. Electrons with energy ≥5eV in the electron group are high-energy electrons. When high-energy electrons collide with gas molecules, excited particles (such as N2*, O2*) de-excite and release ultraviolet photons to form ultraviolet radiation.

[0091] Microorganism inactivation is achieved by directly bombarding the cell membrane with high-energy electrons (5-8eV) to penetrate the peptidoglycan layer and form nano-scale holes in the cell membrane. Ultraviolet radiation induces DNA chain breakage and RNA hydrolysis. Active particles attack the -SH / -OH groups in the active center of the enzyme and the secondary structure of the protein (α helix → β fold conversion), inactivating the protein and thus inactivating the microorganisms.

[0092] Decomposition of ZIF-8 enzymatic hydrolysis products: High-energy electron bombardment directly breaks CC and CN bonds (such as β-1,4 glycosidic bonds of peptidoglycan) in the enzymatic hydrolysis products, generating OH (hydroxyl radicals) and O3 (ozone), which gradually oxidize the macromolecular fragments into CO2 and H2O. By extending the airflow path (3.5m) and the turbulent effect, the product undergoes multiple plasma actions to ensure complete decomposition.

[0093] Through the precise design of polypropylene / barium titanate (BaTiO3) composite fibers, combined with gamma-ray irradiation and bipolar polarization processes, a high-stability electret substrate is constructed. 15% BaTiO3 nanoparticles in the composite fiber increase the dielectric constant to 8.5, and 5% graphene quantum dots (GQDs) form a conductive network to inhibit charge leakage. After 120°C thermal polarization (8kV / mm) and -20kV corona polarization, an electret electrostatic field is formed. A 50nm perfluoropolyether (PFPE) coating is used to achieve a charge half-life of >10 years in an environment with 90% humidity.

[0094] Based on the electret electrostatic field, the alternating electrode array and the piezoelectric ceramic array are integrated to form a multi-physical field synergy mechanism.

[0095] The electrostatic field exerts Coulomb force on charged aerosols (such as viruses and bacteria) with an efficiency of >95%.

[0096] The voltage of a specific frequency is applied through the alternating electrode array to form a non-uniform alternating electric field to induce the polarization migration of neutral particles (PM0.1). Realize positive / negative DEP dynamic capture and fill in the electrostatic field blind area.

[0097] The piezoelectric ceramic array (PZT-5H) drives the aluminum honeycomb substrate at a frequency of 50 to 200 Hz, generating a mechanical wave with an amplitude of 5 to 20 μm, which directionally peels off the particles adsorbed on the surface of the electret fiber. Combined with the reverse airflow (2 m / s), the air flow recovery rate through the electrostatically adsorbed HEPA layer is greater than 95%.

[0098] The nanocatalytic layer achieves the degradation of microorganisms, organic aerosols and volatile organic compounds (VOCs) through the synergy of functional catalytic design, mass transfer enhancement and dynamic oxidation strategy. It is divided into the following three steps:

[0099] 1) Based on the metal-organic framework confinement effect, a ZIF-8-derived carbon layer was constructed on the surface of titanium dioxide (TiO2), forming a molecular sieve structure with a pore size of 1.2 nm. Platinum-cobalt (Pt-Co) bimetallic nanoclusters (particle size 2 nm) were precisely anchored. The Pt-Co nanoclusters served as catalytic active centers and preferentially adsorbed organic aerosols with a molecular weight of 200-500 Da (such as polycyclic aromatic hydrocarbons and lipid particles). The local reactant concentration was increased by 20 times through the confinement enrichment effect, and the surface oxidation rate of titanium dioxide (TiO2) reached 0.5 min- 1 At the same time, the sulfonic acid groups modified on the edge of the carbon layer trigger a chain reaction of hydroxyl radicals (·OH), achieving the step-by-step breakage of long-chain alkanes, and the mineralization rate is increased to 99%.

[0100] 2) Using a hydrophobic-hydrophilic gradient carrier, a polydimethylsiloxane (PDMS) hydrophobic layer is coated on the surface of the three-dimensional graphene foam, and carboxyl (-COOH) hydrophilic groups are grafted onto the inner wall of the mesopores. The hydrophobic region captures non-polar organic aerosols (such as oil mist), and the hydrophilic region adsorbs polar components (such as microbial metabolic acids). The capture efficiency is increased from 60% to 95%. Combined with the Archimedean spiral flow channel design, a 10m / s tangential airflow is applied, and the submicron aerosol is enriched on the catalyst surface through centrifugal force. The residence time is extended to 8 seconds, and the mass transfer efficiency is increased by 3 times.

[0101] 3) Establish a photocatalytic-plasma-ozone-thermocatalytic quadruple dynamic oxidation system, ultraviolet light (365nm, 50mW / cm 2) excites TiO2 to generate OH and superoxide radicals, degrading 90% of formaldehyde within 5 minutes. When polycyclic aromatic hydrocarbons (PAHs) are detected, the dielectric barrier discharge (DBD) is activated to generate high-energy electrons, cracking CC bonds, and the degradation rate of benzopyrene is 99% (15 minutes). Ozone (1ppm) is injected under high humidity (>70% RH) to oxidize microbial envelope lipids, with a virus inactivation rate of >99.99%. Thermal catalysis at 80℃ is started in a dark environment or at night, and the Fe2O3 / ZnO heterojunction activates oxygen to maintain basic oxidation capacity.

[0102] To address catalyst deactivation, an in-situ regeneration strategy was introduced, injecting 0.1% H2O2 vapor every 12 hours to remove carbon deposits, and synchronized 40kHz piezoelectric ceramic micro-vibration to prevent nanoparticle agglomeration. The catalyst life was extended from 200 hours to 2000 hours.

[0103] S2, install a high-sensitivity piezoelectric resonant particle counter behind the 5μm metal filter to detect suspended particles with a diameter of 0.5-20μm to obtain particle concentration. The sampling frequency is 1Hz. It is linked to the turbulence enhancement structure in S1. When a sudden increase in particles >5μm is detected, the filter vibration frequency is triggered to increase to 120Hz.

[0104] ATP bioluminescence detectors were embedded in the ZIF-8 layer outlet and before and after the HEPA layer to reflect the synergistic effect of lysozyme decomposition and plasma inactivation. 3 The discharge time is automatically extended to 3.2ms to inactivate microorganisms.

[0105] Dual-channel temperature and humidity sensors are placed before and after the HEPA layer, with an accuracy of ±1% RH. A compensation model is established based on the humidity sensitivity of the electret material to ensure that the electrostatic adsorption efficiency deviation is less than 3% under 90% RH conditions.

[0106] The VOCs spectrum sensor is integrated in the nanocatalytic oxidation layer to detect the concentration of benzene / formaldehyde etc. at 0.1-100ppm, triggering the photocatalytic intensity graded adjustment (ultraviolet light intensity 50-150mW / cm 2 ),

[0107] S3. A honeycomb structure was made of high-density aluminum alloy (AA6061-T6). The cell aperture was designed to be Φ20±0.1mm, with an aspect ratio of 1:1.2. The surface was anodized to form a 5μm thick Al2O3 insulation layer. Computational fluid dynamics (CFD) simulation was used to determine the optimal guide plate inclination angle (15° at the inlet, 25° at the middle, and 10° at the outlet), achieving a convergence of the airflow diffusion angle from 45° to 5°.

[0108] Add the main and standby air units at the air outlet of the honeycomb. The main fan is an EC backward centrifugal fan with an air volume of 3000m3 / h@0.45m / s and a full pressure of 500Pa. The standby fan is installed in parallel with the same model fan. It is in normal sleep mode (the speed is maintained at 10% standby). The magnetic coupling coupling is used to achieve impact-free switching within 0.1s. It will automatically activate when the main fan current fluctuation is greater than ±15% for 5s or the wind speed sensor detects a deviation of more than 8% from the reference value of 0.45m / s.

[0109] Three layers of adjustable guide components are integrated inside the honeycomb. The upper layer uses 316L stainless steel wire mesh (mesh size 80→120→200 gradient distribution) to achieve primary rectification. The middle layer uses piezoelectric ceramic-driven shutters (adjustment accuracy ±0.5°) to dynamically compensate for turbulence. The lower layer uses 3D-printed nylon guide cones to shape the final flow pattern of the airflow passing through the upper stainless steel wire mesh and the middle piezoelectric shutters to eliminate local eddies.

[0110] The ground guide plate is installed on the ground to correspond to the air outlet of the honeycomb. The ground guide plate adopts gradient density sintered metal porous plate (porosity 65% → 80% → 95% three-level transition), and the pore size distribution meets the following requirements:

[0111]

[0112] Where D is the aperture size, D0 = 0.8 mm is the average aperture, σ = 0.15 is the standard deviation, is the pore size distribution density,

[0113] A linear piezoelectric ceramic driver (stroke 0-50mm, resolution 1μm) is installed at the bottom of the ground deflector. The height of the deflector is dynamically adjusted according to the particle counter data. When the concentration of 0.5-1μm particles is detected to be >10 4 pcs / m 3 When the device is lifted 15 mm, the backflow is enhanced and the microbial activity is >103 CFU / m 3 When lowered by 8mm, the airflow path is extended.

[0114] The bottom of the guide plate is integrated with a periodic release of 0.1MPa compressed air pulses and a UV photocatalytic coating (TiO2 loading 3.2mg / cm 2 ) decomposes deposited organic matter and captures escaping particles using an electrostatic adsorption network (electret material surface potential -1.5kV).

[0115] S4, reverse pulse cleaning adopts a dual-threshold judgment strategy of pressure difference and particle concentration, combining the real-time data of the differential pressure transmitter with the penetration rate monitoring of the particle counter. When the total pressure difference of the three-stage filter reaches 300Pa·s, the cleaning program is triggered. If the penetration rate of 0.3μm particles after the HEPA layer exceeds 5%, emergency cleaning is started simultaneously. The system dynamically adjusts the pulse frequency according to the degree of blockage. For mild blockage (300-500Pa·s), a single 0.5MPa / 100ms pulse is used. For moderate blockage (500-800Pa·s), it switches to three consecutive 0.8MPa / 50ms pulses. For severe blockage (>800Pa·s), a high-frequency 1.2MPa / 30ms pulse sequence is enabled to ensure that particulate matter is removed step by step without damaging the filter material.

[0116] Differentiated pulse parameters are designed based on the characteristics of different filter layers in S1. The 5μm metal filter is impacted by a 0.6MPa reverse 60° airflow for 80ms, using the turbulent effect of the tapered micro-pillar array to shake off large particles. The ZIF-8 layer is subjected to a 0.3MPa tangential 45° pulse for 120ms, and the cyclonic field of the spiral plasma channel assists in removing bioaerosol residues. The HEPA filter paper is subjected to a 0.2MPa normal 90° pulse for 200ms, combining the attenuation characteristics of the electret electrostatic field to directionally remove submicron particles. The pulsed airflow is supplied by dual redundant gas tanks (main tank 20L@1.5MPa, spare tank 15L@1.2MPa).

[0117] After pulse cleaning, wavelength-intensity coupled UV activation was initiated, with a UVC 254 nm light source at 80 mW / cm 2 Irradiate the nanocatalytic layer (TiO2 / graphene heterojunction) to excite hydroxyl radicals (·OH) to decompose organic matter. The UVA 365nm light source is 30mW / cm 2 To assist in activating the lysozyme activity on the ZIF-8 surface, a pulse mode of 5 seconds irradiation / 2 seconds interval was used, and the infrared heating module was used to heat the catalyst layer to 120°C to accelerate the oxidation of carbon deposits. At the same time, 0.5-1ppm ozone was injected to ensure the complete decomposition of by-products through the extended path of the spiral plasma channel in S1.

[0118] During cleaning, the main and backup fans are controlled in a coordinated manner. When a pulse airflow is triggered, the main fan speed is reduced to 60% of the rated speed (air volume 1800m3 / h), and the backup fan speed is increased to 80% (2400m3 / h). The PID controller is used to achieve dynamic balance of the total air volume:

[0119] Q total =Q main +Q backup

[0120] Among them, Q total is the total air volume, Q main The real-time air volume of the main fan, Qbackup The air volume of the standby fan is

[0121] The standby fan outlet is integrated with a pulse buffer chamber (volume 0.5m 3 ), built-in Helmholtz resonator (resonance frequency 20Hz) absorbs pressure pulsation, linked to the S3 guide plate, when the pulse pressure is greater than 0.8MPa, the guide plate switches to turbulence suppression mode,

[0122] If the main fan fails completely, the backup fan switches to 120% overclocking mode (3600m3 / h) for less than 3 minutes, and at the same time activates the electrostatic adsorption enhancement mode of the HEPA filter paper in S1 to compensate for the decrease in filtration efficiency caused by the increase in wind speed.

[0123] The purged particles are treated twice by the spiral plasma channel, increasing the discharge intensity to 15kV / cm and extending the residence time to 5ms, achieving a microbial inactivation rate of >99.99%. After the cleaning is completed, a three-level verification is performed, including pressure difference recovery detection (ΔP < initial value 110%), catalytic efficiency test (toluene degradation rate >98%), and ATP biological activity review (<50RLU).

[0124] The pulse airflow parameters strictly match the mechanical strength and surface characteristics of each level of filter material (such as the impact resistance of the conical micro-columns of the 5μm filter and the crystal surface stability of ZIF-8) to avoid structural damage caused by excessive cleaning.

[0125] During cleaning, the height of the guide plate is automatically adjusted (raising it by 15mm to enhance the return flow or lowering it by 8mm to extend the path), maintaining the uniformity of the air speed in the space at 0.45m / s±0.02m / s.

[0126] The above description is merely a specific embodiment of the present application, but the scope of protection of the present application is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in this application should be included in the scope of protection of this application. Therefore, the scope of protection of this application should be based on the scope of protection of the claims.

[0127] Finally: The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A dust-free environment purification method for biopharmaceuticals, characterized in that: include; S1. Arrange multi-stage filtration system in sequence along the airflow direction. The first stage is equipped with three layers of metal filter screens, on the surface of which a conical micro-pillar array, an Al2O3 / TiO2 composite ceramic layer and a diamond-like carbon film are respectively provided to intercept suspended particles larger than 1 μm. The second stage uses ZIF-8 metal organic framework material with crystal surface regulation, and the surface is cross-linked with glutaraldehyde to fix lysozyme, combined with dielectric barrier discharge of spiral plasma channel to inactivate microorganisms. The third level sets up electrostatic adsorption HEPA filter paper to capture 0.1-1μm particles. Its electret substrate contains 15% BaTiO3 nanoparticles and 5% graphene quantum dots. The fourth stage uses a TiO2 / graphene heterostructured nanocatalytic layer to decompose organic aerosols <0.1μm through Pt-Co bimetallic nanoclusters and a quadruple dynamic oxidation system; S2. Multi-parameter coordinated monitoring and adjustment: deploy a differential pressure transmitter and a particle counter at the three-layer metal filter, and combine it with an ATP bioluminescence meter and a VOCs spectrum sensor to obtain real-time data on pollutants in the environment; S3, laminar flow control and dynamic compensation: a honeycomb air outlet is set at the end of the multi-stage filtration system, and a gradient guide plate inclination is used to form a uniform laminar flow. A porosity sintered metal guide plate is arranged on the ground, and the height is dynamically adjusted in combination with a piezoelectric ceramic driver to achieve intelligent adjustment of the direction and speed of the airflow, avoiding secondary contamination of the already purified area. S4, pulse cleaning and catalytic regeneration: When the differential pressure integral in the three-stage filter reaches 300 Pa·s, a graded reverse pulse purge is initiated to purge the filter, and ultraviolet pulses are simultaneously activated to decompose carbon deposits. During cleaning, the main and standby fans are coordinated and controlled, and the total air volume balance is maintained through a PID algorithm. The purged particles pass through the spiral plasma channel for secondary treatment.

2. A dust-free environment purification method for biopharmaceuticals according to claim 1, characterized in that: The three-layer metal filter is made of 316L stainless steel through a photolithography etching process. The three-layer metal filter includes 5μm, 3μm and 1μm. Different surface treatments are performed on each layer of filter. The 5μm filter is prepared with a conical micro-pillar array with a height of 3μm and a cone angle of 60° on the surface. The 3μm filter is formed by micro-arc oxidation to form an Al2O3 / TiO2 composite ceramic layer. The 1μm filter is deposited with a diamond-like carbon film by magnetron sputtering. The optimal spacing between different filter screens of the three-layer metal filter screen is determined through computer simulation prediction, laboratory measurement verification and actual scenario fine-tuning.

3. A dust-free environment purification method for biopharmaceuticals according to claim 1, characterized in that: The ZIF-8 metal organic framework material adopts a solvent thermal method to grow ZIF-8 crystals on an a-Al2O3 substrate, and through crystal face control, a cubic structure with a {100} crystal face ratio of more than 85% is obtained, which is used to intercept microorganisms with a diameter greater than 3.

43. The surface of the ZIF-8 metal organic framework material was constructed with an amino surface using 3-aminopropyltriethoxysilane, and lysozyme was immobilized by glutaraldehyde cross-linking. The cross-linker concentration followed the optimal lysozyme activity equation: A=0.82[G] 2 -1.75[G]+0.95(0.2%≤[G]≤1.0%) When the optimal concentration [G] is determined to be 0.5%, the lysozyme activity retention rate is greater than 92%. The intercepted microorganisms are enzymatically hydrolyzed by lysozyme. The spiral plasma channel adopts a double-helix coaxial structure to meet the residence time required for dielectric barrier discharge. High-voltage electrodes and ground electrodes are installed at the inlet, middle and outlet of the inner and outer spirals of the spiral plasma channel, respectively. A tangential swirler is set at the air inlet to form a turbulent flow with a Reynolds number Re>4500. The turbulent flow velocity is controlled by a three-section variable frequency centrifugal fan. Under the action of high-voltage electrodes, the dielectric barrier discharge causes the gas between the electrodes to be broken down and ionized, causing electrons to accelerate in the strong electric field and collide with neutral molecules to produce an avalanche effect, forming a high-density electron group. The electron avalanche effect decomposes H2O / O2 to generate free radicals, ozone and other active particles. Electrons with energy ≥5eV in the electron group are high-energy electrons. When high-energy electrons collide with gas molecules, the excited state particles de-excite and release ultraviolet photons to form ultraviolet radiation. The microorganisms are inactivated by directly bombarding the cell membrane with high-energy electrons to penetrate the peptidoglycan layer and form nanoscale holes in the cell membrane. The ultraviolet radiation induces DNA chain breakage and RNA hydrolysis. The active particles attack the -SH / -OH groups in the active center of the enzyme and the secondary structure of the protein, thereby inactivating the protein and thus causing the inactivation of the microorganisms. Decomposition of ZIF-8 enzymatic hydrolysis products: The high-energy electron bombardment directly breaks the CC and CN bonds in the enzymatic hydrolysis products, generates OH hydroxyl radicals and O3 ozone, and gradually oxidizes the macromolecular fragments into CO2 and H2O. Through the turbulent effect, the enzymatic hydrolysis products undergo multiple plasma actions to ensure complete decomposition.

4. A dust-free environment purification method for biopharmaceuticals according to claim 1, characterized in that: The electret substrate is constructed through the precise design of polypropylene / barium titanate (BaTiO3) composite fibers, combined with gamma ray irradiation and bipolar polarization processes to construct a high-stability electret substrate. 15% of BaTiO3 nanoparticles in the composite fibers increase the dielectric constant to 8.5, and 5% of graphene quantum dots (GQDs) form a conductive network to inhibit charge leakage. After 120°C thermal polarization and -20kV corona polarization, an electret electrostatic field is formed, and a 50nm perfluoropolyether (PFPE) coating is applied to the surface of the electret substrate. The electret electrostatic field forms a multi-physical field synergy mechanism by integrating the alternating electrode array and the piezoelectric ceramic array. 1) The electrostatic field exerts Coulomb force on the charged aerosol. 2) Applying a voltage of a specific frequency through an alternating electrode array to form a non-uniform alternating electric field to induce polarization migration of neutral particles, and realizing positive / negative DEP dynamic capture through the non-uniform electric field gradient to fill the electrostatic field blind area. 3) The piezoelectric ceramic array drives the aluminum honeycomb substrate at a frequency of 50 to 200 Hz, generating a mechanical wave with an amplitude of 5 to 20 μm, which directionally peels off the particles adsorbed on the surface of the electret substrate.

5. A dust-free environment purification method for biopharmaceuticals according to claim 1, characterized in that: The nanocatalytic layer achieves the degradation of microorganisms, organic aerosols and volatile organic compounds through the synergy of functional catalytic design, mass transfer enhancement and dynamic oxidation strategy, which is divided into the following three steps: 1) Based on the metal-organic framework confinement effect, a ZIF-8-derived carbon layer was constructed on the titanium dioxide surface to form a molecular sieve structure, precisely anchoring platinum-cobalt bimetallic nanoclusters. The platinum-cobalt bimetallic nanoclusters acted as the active center of the catalyst, preferentially adsorbing organic aerosols with a molecular weight of 200-500 Da. The local reactant concentration was increased through the confinement enrichment effect, and the surface oxidation rate of titanium dioxide reached 0.5 min -1 At the same time, the sulfonic acid groups modified at the edge of the carbon layer trigger a chain reaction of hydroxyl radicals, achieving the step-by-step breakage of long-chain alkanes and improving the mineralization rate of the organic aerosol. The mineralization refers to the process of completely decomposing organic pollutants into inorganic small molecules through oxidation reactions. 2) Using a hydrophobic-hydrophilic gradient carrier, a polydimethylsiloxane hydrophobic layer is coated on the surface of the three-dimensional graphene foam, and carboxyl hydrophilic groups are grafted onto the inner walls of pores with a pore size of 2-50 nm. The hydrophobic region captures non-polar organic aerosols, and the hydrophilic region adsorbs polar components, greatly improving the capture efficiency. Combined with the Archimedean spiral flow channel design, a 10 m / s tangential airflow is applied, and submicron aerosols are enriched on the surface of the catalyst through centrifugal force, extending the residence time of the submicron aerosols to 8 seconds, thereby improving the mineralization rate. 3) Establish a four-fold dynamic oxidation system of photocatalysis, plasma, ozone, and thermal catalysis. Ultraviolet light excites TiO2 to generate OH and superoxide radicals, degrading 90% of formaldehyde within 5 minutes. When polycyclic aromatic hydrocarbons are detected, dielectric barrier discharge is activated to generate high-energy electrons, cleaving CC bonds, resulting in a 99% degradation rate of benzopyrene. Ozone is injected under high humidity to oxidize microbial envelope lipids, achieving a virus inactivation rate of >99.99%. Thermal catalysis is activated at 80°C in a dark environment or at night, where the Fe2O3 / ZnO heterojunction activates oxygen to maintain basic oxidation capacity. To address the deactivation of the catalyst, an in-situ regeneration strategy was introduced, injecting 0.1% H2O2 steam every 12 hours to remove carbon deposits, and synchronous 40kHz piezoelectric ceramic micro-vibration to prevent nanoparticle agglomeration, thereby extending the catalyst life from 200 hours to 2000 hours.

6. A dust-free environment purification method for biopharmaceuticals according to claim 1, characterized in that: The multi-parameter coordinated monitoring and regulation is achieved by deploying embedded differential pressure transmitters before and after each layer of the three-layer metal filter. The measuring range is divided into different levels by layer. The embedded differential pressure transmitter is used to measure the pressure difference between the front and rear ends of each level of filter. The pressure difference between the front and rear ends of each level of filter is a pressure differential signal. The Poiseuille equation is used to reversely infer the change in the effective pore ratio of the filter participating in particle interception, thereby accurately evaluating the degree of filter blockage. A high-sensitivity piezoelectric resonant particle counter is installed behind each level of the three-layer metal filter to detect suspended particulate matter with a diameter of 0.5-20μm. The sampling frequency is 1Hz. It is linked to the turbulence enhancement structure in S1. When a sudden increase in particles >5μm is detected, the filter vibration frequency is triggered to increase to 120Hz. The high-frequency mechanical vibration removes large particle pollutants accumulated on the filter surface, preventing filter clogging and maintaining the system purification efficiency. An ATP bioluminescence detector is embedded in the ZIF-8 layer outlet and in front of and behind the HEPA filter paper. The ATP bioluminescence detector is used to detect the activity of microorganisms in the current environment. The synergistic effect of lysozyme decomposition and plasma inactivation is reflected by the microbial activity. When the microbial activity is greater than 10 CFU / m 3 Automatically extend the discharge time to inactivate microorganisms. Dual-channel temperature and humidity sensors are placed before and after the HEPA layer to detect the temperature and humidity in the current environment. A compensation model is established based on the humidity sensitivity of the electret material. A VOCs spectral sensor is integrated into the nano-catalytic oxidation layer to detect the concentration of 0.1-100ppm of benzene / formaldehyde, etc., triggering graded adjustment of photocatalytic intensity.

7. A dust-free environment purification method for biopharmaceuticals according to claim 1, characterized in that: The honeycomb air outlet is made of a high-density aluminum alloy to make a honeycomb structure. The air outlet of the honeycomb structure is integrated with a wind speed sensor, which is used to detect the wind speed of the air outlet. The honeycomb structure is provided with piezoelectric ceramic driven guide plates at the inlet, middle and outlet. The surface of the honeycomb structure is anodized to form a 5 μm thick Al2O3 insulating layer. The optimal piezoelectric ceramic driven guide plate inclination angle is determined by computational fluid dynamics simulation to achieve the convergence of the airflow diffusion angle from 45° to 5°, optimize the uniformity of laminar flow and suppress turbulence. The main and standby air units are added to the air outlet of the honeycomb. The main fan is an EC backward centrifugal fan, and the standby fan is installed in parallel with the same model fan. The normal state is dormant, and the magnetic coupling coupling is used to achieve impact-free switching within 0.1s. Three layers of adjustable guide components are integrated inside the honeycomb. The upper layer uses 316L stainless steel wire mesh to achieve primary rectification, the middle layer uses piezoelectric ceramic driven shutters to dynamically compensate for turbulence, and the lower layer uses 3D printed nylon guide cones. The Indonesian dragon guide cones shape the final flow pattern of the airflow passing through the upper stainless steel wire mesh and the middle piezoelectric shutters to eliminate local eddies. The ground guide plate is installed on the ground to correspond to the air outlet of the honeycomb. The ground guide plate adopts gradient density sintered metal porous plate to achieve precise control and dynamic adaptability of air flow distribution. A particle counter and a linear piezoelectric ceramic driver are installed on the ground guide plate, and the height of the ground guide plate is dynamically adjusted according to the particle counter data. A periodic release of 0.1 MPa compressed air pulses and an ultraviolet catalytic coating are integrated at the bottom of the ground-mounted ground guide plate. The periodic release of 0.1 MPa compressed air pulses removes particulate matter attached to the ground guide plate, and the ultraviolet catalytic coating oxidizes and decomposes organic pollutants attached to the surface of the guide plate into CO2 and H2O.

8. A dust-free environment purification method for biopharmaceuticals according to claim 1, characterized in that: The reverse pulse cleaning adopts a pressure difference-particle concentration dual threshold judgment strategy, combines the real-time data of the differential pressure transmitter with the data of the particle counter, and dynamically adjusts the pulse frequency according to the degree of blockage. Differentiated pulse parameters are designed according to the characteristics of different filter layers. The 5μm metal filter is subjected to a 0.6MPa reverse 60° airflow for 80ms, and the turbulence effect of the conical micro-pillar array is used to shake off large particles. The ZIF-8 layer is subjected to a 0.3MPa tangential 45° pulse for 120ms, and the cyclonic field of the spiral plasma channel assists in removing bioaerosol residues. The HEPA filter paper is subjected to a 0.2MPa normal 90° pulse for 200ms, combined with the attenuation characteristics of the electret electrostatic field to directionally remove submicron particles. The pulsed airflow is supplied by dual redundant gas tanks. The pressure differential integral is the total amount of pressure differential accumulated over time on both sides of each level of filter screen, and is calculated using the following formula: Among them, P(t) is the real-time measured pressure difference before and after each level of filter, t is time (unit: second), The reverse pulse cleaning also includes setting a bypass pipe at the cleaning outlet of the three-stage metal filter, ZIF-8 layer and HEPA filter paper, isolating the peeling particles from the main airflow through a three-way valve, installing a negative pressure air pump at the starting point of the bypass pipe, and transporting the particles through the bypass pipe to the spiral plasma channel for secondary processing. The normal air inlet of the spiral plasma channel is closed during cleaning. The catalytic regeneration is achieved by starting wavelength-intensity coupled ultraviolet activation after pulse cleaning. Ultraviolet C (UVC) irradiates the nanocatalytic layer to stimulate hydroxyl radicals to decompose organic matter. Long-wave dark spot effect ultraviolet (UVA) assists in activating the lysozyme activity on the ZIF-8 surface. A pulse mode of 5 seconds irradiation / 2 seconds interval is used, and an infrared heating module is used to heat the catalytic layer to 120°C to accelerate the oxidation of carbon deposits. At the same time, 0.5-1ppm ozone is injected to ensure complete decomposition of by-products through the extended path of the spiral plasma channel. The extended path refers to the increased flow distance of the airflow in the plasma channel of the double-helix coaxial structure due to the spiral trajectory. During cleaning, the main and standby fans are controlled in coordination. When the pulse airflow is triggered, the main fan speed is reduced to 60% of the rated speed, and the standby fan speed is increased to 80% simultaneously. The standby fan outlet is integrated with a pulse buffer chamber, a built-in Helmholtz resonator to absorb pressure pulsations, and a linkage with the ground guide plate. When the pulse pressure is greater than 0.8 MPa, the guide plate switches to turbulence suppression mode. If the main fan fails completely, the backup fan switches to 120% overclocking mode for less than 3 minutes, and at the same time activates the electrostatic adsorption enhancement mode of the HEPA filter paper in S1 to compensate for the decrease in filtration efficiency caused by the increase in wind speed. The pulse airflow parameters strictly match the mechanical strength and surface characteristics of each level of filter material. During cleaning, the height of the ground guide plate is automatically adjusted to maintain the uniformity of air speed in the space at 0.45m / s±0.02m / s.

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