Feed additive for improving recovery capability of equine animals after movement and application thereof

Abstract

The invention discloses a feed additive for improving recovery capability of equine animals after exercise. The feed additive comprises a formula I and a formula II, wherein the formula I consists of astragalus membranaceus and codonopsis pilosula, and the formula II consists of atractylodes macrocephala koidz and pericarpium citri reticulatae. The invention also discloses an application of the feed additive for improving the recovery capability of the equindae animals after exercise in the feed of the exercise horses. The application comprises the step of adding the feed additive into the basal feed of the exercise horses according to the proportion of 8-20%. The sports horse feed can be continuously fed in horse racing competitive sports, the basic energy source needed by horse racing is guaranteed, meanwhile, the feed additive capable of improving the recovery capacity of horse animals after sports is added, then the explosive power and persistence of horse sports can be kept, and the utilization rate of the feed is higher.

Description

Technical Field

[0001] The present invention relates to the field of feed additives, and in particular to a feed additive for improving the recovery ability of equines after exercise and an application thereof. Background Art

[0002] As herbivorous animals, horses were domesticated by humans approximately 4,000 years ago and played a vital role in agriculture, transportation, and military power in ancient society. However, with the Industrial Revolution, when mechanical power replaced traditional animal power, coupled with innovations in modern agricultural technology, the value of horses' labor gradually diminished, and their use shifted to equestrian competitions and performances.

[0003] Large-scale horse racing began in Britain in the 17th century, and the breeding of sport horses gradually gained attention in more regions around the world. As one of humanity's oldest racing sports, horse racing has always been based on speed as its core evaluation criterion, and is inextricably linked to the horse's explosive power, endurance, hoof structure, breed genetics, and pedigree. These factors are closely related to the daily care of the horse.

[0004] Currently, the feeding of modern sports horses has gradually shifted from the traditional extensive model to a scientific and precise feeding system, that is, using high-energy-density concentrated feed (such as grains and beans) as the core nutritional source, supplemented by an appropriate amount of roughage (hay, silage) to regulate gastrointestinal digestion. It is worth noting that for sports horses, high-intensity training is prone to problems such as qi deficiency, fatigue, muscle fatigue, and difficulty in rapid physical recovery. Based on this, the present application proposes a feed additive that improves the recovery ability of equines after exercise and the use of this additive in sports horse feed. Summary of the Invention

[0005] In order to at least partially address the deficiencies in the prior art, the main purpose of the present invention is to provide a feed additive for improving the recovery ability of equines after exercise and its application.

[0006] In order to achieve the above main objectives, the first aspect of the present invention discloses a feed additive for improving the recovery ability of equines after exercise, the feed additive comprising Formula No. 1 and Formula No. 2; wherein, Formula No. 1 is composed of Astragalus and Codonopsis, and Formula No. 2 is composed of Atractylodes and Citrus Reticulata.

[0007] In the above technical solution of the present invention, the Astragalus and Codonopsis in Formula No. 1 are mainly used to increase the oxygen-carrying capacity of red blood cells after being absorbed, and endurance is significantly improved, delaying fatigue of exercise horses and significantly improving their recovery speed after exercise; the Atractylodes and Tangerine Peel in Formula No. 2 can help convert basic feed into energy, reduce the accumulation of metabolic waste and significantly improve the recovery speed after exercise.

[0008] According to a specific embodiment of the present invention, the mass ratio of Formula No. I to Formula No. II is (1-4): (3-6).

[0009] According to a specific embodiment of the present invention, the mass ratio of Astragalus membranaceus and Codonopsis pilosula in Formula No. Ⅰ is (1-4): (1-4), and the mass ratio of Atractylodes macrocephala and Citrus reticulatae in Formula No. Ⅱ is (1-2): (2-4).

[0010] According to a specific embodiment of the present invention, the Astragalus is roasted Astragalus, and the Atractylodes is stir-fried Atractylodes.

[0011] According to a specific embodiment of the present invention, the feed additive further comprises Formula III, which consists of hawthorn and liquorice.

[0012] Furthermore, the hawthorn and licorice in Formula III help promote protein breakdown and accelerate muscle repair; licorice harmonizes electrolyte balance, prevents dehydration or electrolyte imbalance, and thus helps improve the metabolism of equines after exercise, ultimately improving recovery ability.

[0013] According to a specific embodiment of the present invention, the mass ratio of hawthorn and licorice in formula III is (1-5): (1-5).

[0014] According to a specific embodiment of the present invention, the mass proportion of Formula III in the feed additive is less than 15%.

[0015] The second aspect of the present invention provides a use of the feed additive for improving the recovery ability of equine animals after exercise in a sports horse diet, which comprises adding the feed additive to the basic feed of the sports horse at a ratio of 8%-20%.

[0016] According to a specific embodiment of the present invention, complex amino acids are added to the basic feed of sports horses at a ratio of 2%-4%, wherein the complex amino acids consist of lysine, methionine, threonine, tryptophan and glutamine.

[0017] According to a specific embodiment of the present invention, micronutrients are added to the basic feed of sports horses at a ratio of 0.5%-2%; wherein the micronutrients include minerals and vitamins, the minerals include at least one of yeast selenium, potassium carbonate, calcium hydrogen phosphate, sodium chloride and magnesium sulfate, and the vitamins include at least one of vitamin A, vitamin B, vitamin D3, vitamin K, vitamin E and niacinamide.

[0018] In order to more clearly illustrate the purpose, technical solutions and advantages of the present invention, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1is a diagram showing the result indicators of red blood cells (RBC) 2 h before exercise, 0.5 h after exercise, and 24 h after exercise in each group of horses;

[0020] Figure 2 It is a diagram showing the result indicators of white blood cells (WBC) of horses in each group 2 hours before exercise, 0.5 hours after exercise, and 24 hours after exercise;

[0021] Figure 3 is a schematic diagram showing the result indicators of neutrophils (NE) in horses of each group 2 h before exercise, 0.5 h after exercise, and 24 h after exercise;

[0022] Figure 4 It is a diagram showing the result indicators of alanine aminotransferase (ALT) 2 h before exercise, 0.5 h after exercise, and 24 h after exercise in horses of each group;

[0023] Figure 5 is a diagram showing the result indicators of aspartate aminotransferase (AST) of horses in each group 2 hours before exercise, 0.5 hours after exercise, and 24 hours after exercise;

[0024] Figure 6 is a diagram showing the result indicators of creatinine (Cr) 2 h before exercise, 0.5 h after exercise, and 24 h after exercise in horses of each group;

[0025] Figure 7 is a diagram showing the result indicators of urea nitrogen (BUN) 2 h before exercise, 0.5 h after exercise, and 24 h after exercise in horses of each group;

[0026] Figure 8 is a diagram showing the result indicators of creatine kinase (CK) 2 h before exercise, 0.5 h after exercise, and 24 h after exercise in horses of each group;

[0027] Figure 9 It is a diagram showing the result indicators of lactate dehydrogenase (LDH) of horses in each group 2 hours before exercise, 0.5 hours after exercise, and 24 hours after exercise. DETAILED DESCRIPTION

[0028] In order to more clearly understand the purpose, features and advantages of the present invention, the present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments. In the following description, many specific details are set forth in conjunction with the examples to facilitate a full understanding of the present invention, but it should be understood that the following examples and detailed description are only for illustrative purposes and do not limit the scope of protection of the present invention.

[0029] The present invention provides a feed additive for improving the post-exercise recovery ability of equines, comprising Formula I and Formula II; Formula I comprising astragalus and codonopsis, and Formula II comprising atractylodes and tangerine peel. Preferably, the astragalus is roasted astragalus, and the atractylodes is stir-fried atractylodes.

[0030] As a further preference, the mass ratio of Formula No. Ⅰ and Formula No. Ⅱ in the present invention is (1-4): (3-6), the mass ratio of Astragalus and Codonopsis in Formula No. Ⅰ is (1-4): (1-4), and the mass ratio of Atractylodes and Tangerine Peel in Formula No. Ⅱ is (1-2): (2-4).

[0031] The feed additive further comprises Formula III, which is composed of hawthorn and licorice. The mass ratio of hawthorn to licorice in Formula III is preferably (1-5): (1-5).

[0032] As a further preference, the mass proportion of Formula III in the feed additive is less than 15%.

[0033] Chinese herbal medicine is a natural product that contains a variety of effective ingredients. It basically has all the functions of feed additives and can be used as an independent type of feed additive. It also has the characteristics of being relatively inexpensive, non-toxic, non-side effects, and non-drug-resistant.

[0034] Astragalus: It is slightly warm in nature, sweet in flavor, and enters the spleen and lung meridians. It has the functions of tonifying qi and raising yang, strengthening the spleen and stomach's transportation and transformation functions, and is used to relieve qi deficiency fatigue and loss of appetite; it is used for middle qi sinking (such as visceral prolapse, chronic diarrhea and rectal prolapse); it strengthens the exterior and stops sweating, and is used to improve the body's surface defensive qi and treat spontaneous sweating and night sweats; it is used to promote diuresis and reduce swelling, and is used to promote water metabolism and improve edema caused by qi deficiency; it is commonly used for qi deficiency-induced fatigue and low immunity.

[0035] Raw Astragalus root is stir-fried with honey to enhance its warming and nourishing properties, which is called roasted Astragalus root. It has a stronger effect of tonifying the middle and replenishing qi. Honey synergistically enhances the spleen-strengthening effect. It can also moisten the lungs, relieve coughs, nourish blood and calm the mind. Modern pharmacological research shows that Astragalus polysaccharides can regulate immunity and resist fatigue. After roasting with honey, the flavonoid content increases, enhancing antioxidant activity.

[0036] Codonopsis pilosula: It is sweet and neutral in nature, and enters the spleen and lung meridians. It strengthens the spleen and lungs, tonifies the middle qi, and can enhance the spleen and stomach's transport and transformation functions, improving appetite, abdominal distension, and loose stools (often combined with Atractylodes macrocephala and Poria cocos). It also nourishes lung qi, relieving cough, asthma, shortness of breath, and laziness caused by lung deficiency (combined with Astragalus membranaceus and Schisandra chinensis). It also promotes fluid production, nourishes blood, and replenishes blood deficiency, regulating immunity. Modern research shows that Codonopsis pilosula polysaccharides can enhance macrophage activity and improve the body's disease resistance.

[0037] Atractylodes macrocephala:It is warm in nature, bitter and sweet in flavor, and enters the spleen and stomach meridians. It strengthens the spleen and replenishes Qi, improving symptoms such as loss of appetite, fatigue, and abdominal distension caused by spleen deficiency; expels dampness and promotes diuresis, treating dampness-suppressed spleen and stomach, edema, and dysuria; and relieves sweating and stabilizes pregnancy, assisting in the treatment of spontaneous perspiration due to qi deficiency and restless fetal movement during pregnancy. When used raw, it has a strong dampness-removing effect and is suitable for those with damp-heat constitutions or those with severe dampness. However, it should be used with caution in those with spleen and stomach yin deficiency.

[0038] Stir-fried Atractylodes macrocephala (earth-fried Atractylodes macrocephala): Slightly warm in nature, sweet in flavor, and enters the spleen and stomach meridians. It strengthens and regulates spleen and stomach function, alleviating spleen deficiency, poor appetite, and indigestion. It replenishes qi and nourishes the middle qi, making it particularly suitable for those with qi deficiency, fatigue, or chronic illness. It also alleviates dampness and dampness: the high-temperature stir-frying reduces the amount of volatile oil, lessening its dampness-removing properties and making it more gentle on spleen and stomach deficiency and coldness. Compared to Atractylodes macrocephala, stir-fried Atractylodes macrocephala has a milder nature and is better absorbed, making it particularly suitable for those with weak spleen and stomach and prone to diarrhea.

[0039] Tangerine peel: Regulates Qi and strengthens the spleen, relieves abdominal distension, promotes the digestion of the spleen and stomach, and improves abdominal distension and loss of appetite caused by Qi stagnation. Regulates indigestion: Commonly used for abdominal distension, belching and acid reflux after greasy meals. Drys dampness and resolves phlegm, relieves coughs and expectorants: Relieves coughs with excessive phlegm (especially white phlegm or cold phlegm), such as chronic bronchitis and asthma. Improves dampness: Suitable for damp-heat constitutions with thick and greasy tongue coating, fatigue, and sticky stools. Antioxidant: Contains volatile oils (such as limonene) that help scavenge free radicals in the body. Regulates immunity: Enhances the barrier function of the intestinal mucosa and assists in anti-inflammatory treatment.

[0040] Licorice: It is neutral in nature, sweet in flavor, and enters the Heart, Lung, Spleen, and Stomach meridians. It tonifies the spleen and replenishes Qi, relieving fatigue, loss of appetite, and loose stools caused by spleen deficiency. It clears heat and detoxifies, treating ulcers caused by heat, sore throats, carbuncles, and furuncles. It relieves coughs and expectorants, particularly those caused by cold or damp phlegm. It also soothes and relieves pain, harmonizing the spleen and stomach, relieving acute abdominal cramps or cramps in the limbs. Raw licorice root is good at clearing heat and detoxifying, but has weaker dampness-drying properties, but may irritate the stomach and intestines when used raw.

[0041] Roasted licorice root, also known as honey-roasted licorice root, is slightly warm in nature and sweet in flavor. It enters the heart, lung, and spleen meridians. It strengthens the spleen and stomach, tonifying qi and replenishing qi, and improves spleen deficiency, poor appetite, and fatigue. It nourishes yin and moistens the lungs, alleviating dry lung coughs, dry throat, and tongue. It relieves urgency and calms the mind, treating palpitations and insomnia, and harmonizing the qi and blood of the heart and spleen. It harmonizes various herbs, retaining the harmonizing effects of raw licorice but with a milder nature. Roasted licorice root is a warming tonic with less dampness-drying properties, making it suitable for those with spleen and stomach deficiency and qi and blood deficiency.

[0042] hawthorn:It promotes digestion, alleviating abdominal distension and belching caused by greasy meat and starchy foods (such as rice and pasta). It improves appetite by stimulating the secretion of digestive enzymes and enhancing the spleen and stomach's digestive function. It promotes blood circulation and removes blood stasis, dredging the blood vessels and relieving dysmenorrhea, amenorrhea, and postpartum abdominal pain caused by blood stasis. It also aids in lipid reduction by reducing blood viscosity and preventing arteriosclerosis. It can help with traumatic injuries by accelerating the dissipation of blood stasis through external application or internal administration. It promotes qi circulation and relieves bloating, alleviating abdominal distension caused by gastrointestinal stagnation by regulating qi.

[0043] The present invention also provides an application of a feed additive for improving the recovery ability of equine animals after exercise in a sports horse feed. The application comprises adding the feed additive to the basic feed of the sports horse at a ratio of 8%-20%.

[0044] As a further preference, complex amino acids can be added to the sports horse feed. Specifically, the complex amino acids can be added to the basic feed of the sports horse at a ratio of 2%-4%, wherein the complex amino acids are composed of lysine, methionine, threonine, tryptophan and glutamine.

[0045] Among them, the proportions (by mass) of each component in the complex amino acid added to the sports horse feed are preferably as follows: lysine 0.8-1.5 parts, methionine 0.3-0.6 parts, threonine 0.4-0.7 parts, tryptophan 0.15-0.4 parts, and glutamine 0.3-0.8 parts.

[0046] As a further preference, trace nutrients may be added to the sports horse feed, specifically, the trace nutrients may be added to the basic feed of the sports horse at a ratio of 0.5%-2%; wherein the trace nutrients include minerals and vitamins, the minerals include at least one of yeast selenium, potassium carbonate, calcium hydrogen phosphate, sodium chloride and magnesium sulfate, and the vitamins include at least one of vitamin A, vitamin B, vitamin D3, vitamin K, vitamin E and niacinamide.

[0047] Among them, the proportions (by mass) of each component in the trace nutrients added to the sports horse feed are preferably as follows: yeast selenium 0.01-0.02 parts, potassium carbonate 0.1-0.2 parts, calcium hydrogen phosphate 0.1-0.2 parts, sodium chloride 0.1-0.2 parts, magnesium sulfate 0.08-0.2 parts, vitamin A 0.1-0.2 parts, vitamin B complex 0.1-0.2 parts, vitamin D3 0.1-0.2 parts, vitamin K 0.1-0.2 parts, vitamin E 0.1-0.2 parts, niacinamide 0.001-0.002 parts; wherein, the vitamin B complex includes vitamin B1 0.05-0.08 parts, vitamin B2 0.05-0.06 parts, vitamin B6 0.04-0.05 parts, and biotin 0.01-0.03 parts.

[0048] The basic feed for sports horses includes the following ingredients: roasted soybeans, raw oat kernels, flaked corn, alfalfa, bran, vegetable oil, roasted flaxseed, black beans, cane molasses, peanuts, carrot powder, apple powder, and sunflower seeds. The basic feed, by mass, includes 12-18 parts roasted soybeans, 3-6 parts raw oat kernels, 26-35 parts flaked corn, 2-7 parts alfalfa, 3-7 parts bran, 2-5 parts vegetable oil, 3-8 parts roasted flaxseed, 4-6 parts black beans, 3-5 parts cane molasses, 4-6 parts peanuts, 0.5-1 part carrot powder, 0.5-1 part apple powder, and 5-8 parts sunflower seeds.

[0049] The sports horse feed of the present invention can be continuously fed during horse racing. While ensuring the basic energy source required by the racehorse, it is added with feed additives that can improve the recovery ability of equine animals after exercise, thereby helping to maintain the horse's explosive power and endurance, and the feed utilization rate is higher.

[0050] Furthermore, the combination of roasted Astragalus, Codonopsis, and Licorice root forms the "Qi-tonifying Core," tonifying Qi and strengthening the spleen, enhancing physical performance. Astragalus replenishes Qi and raises Yang, Codonopsis replenishes Qi and promotes fluid production, and Licorice root harmonizes the various herbs and tonifies the spleen, synergistically cleansing the intestines and promoting Qi and blood production. For sports horses, this can alleviate Qi deficiency and fatigue after intense training, accelerate recovery, and reduce muscle fatigue.

[0051] Stir-fried Atractylodes macrocephala + Tangerine peel: Promotes qi circulation, relieves stagnation, and eliminates fullness. It strengthens the spleen, regulates qi, and improves digestion. Atractylodes macrocephala nourishes the spleen and eliminates dampness, while tangerine peel regulates qi and relieves fullness. Flowing qi promotes bowel movement. For exercise horses, this can prevent flatulence and diarrhea caused by feed accumulation and optimize nutrient absorption.

[0052] Hawthorn: It dispels qi and resolves bloating, clears gastrointestinal stagnation, and promotes blood circulation, removing blood stasis, and relieving strain. Its sweet and sour flavor stimulates appetite, promotes gastric acid secretion, and dissolves greasiness. By promoting blood circulation and removing blood stasis, it improves local circulation. For exercise horses, it can reduce joint swelling and muscle soreness, and accelerate post-exercise lactic acid metabolism.

[0053] For sports horses, the use of feed additives can achieve the following effects:

[0054] 1) Optimize post-exercise metabolism, promote protein decomposition, accelerate muscle repair; regulate electrolyte balance, and prevent dehydration or electrolyte imbalance;

[0055] 2) Improve endurance and recovery speed: Increase the oxygen-carrying capacity of red blood cells and delay fatigue; promote the efficient conversion of feed into energy and reduce the accumulation of metabolic waste;

[0056] 3) Prevent digestive system diseases: stabilize gastrointestinal function and reduce stress response;

[0057] 4) Enhance immunity: activate immune cell activity and assist in resisting oxidative stress.

[0058] The sports horse feed of the present invention will be further described below with reference to specific examples.

[0059] Among them, the components and mass proportions of the basic feed for sports horses are as follows: 15 parts of roasted soybeans, 5 parts of raw oat kernels, 27 parts of flaked corn, 5 parts of alfalfa, 5 parts of bran, 2 parts of vegetable oil, 5 parts of roasted flax seeds, 5 parts of black beans, 3 parts of sugarcane molasses, 5 parts of peanuts, 1 part of carrot powder, 1 part of apple powder, and 5 parts of sunflower seeds.

[0060] The components of the micronutrients and their mass parts are as follows: 0.02 parts of yeast selenium, 0.2 parts of potassium carbonate, 0.2 parts of calcium hydrogen phosphate, 0.15 parts of sodium chloride, 0.1 parts of magnesium sulfate, 0.1 parts of vitamin A, 0.2 parts of vitamin B complex, 0.2 parts of vitamin D3, 0.1 parts of vitamin K, 0.1 parts of vitamin E, and 0.002 parts of niacinamide.

[0061] The components of the complex amino acid and their weight parts are as follows: 1.3 parts of lysine, 0.5 parts of methionine, 0.6 parts of threonine, 0.4 parts of tryptophan, and 0.7 parts of glutamine.

[0062] Example 1: The feed additive in Example 1 includes 3 parts of roasted astragalus, 2 parts of codonopsis, 2 parts of stir-fried atractylodes, 2.6 parts of tangerine peel, 0.2 parts of roasted liquorice, and 0.2 parts of hawthorn.

[0063] Example 2: The feed additive in Example 2 includes 4.5 parts of roasted astragalus, 1.5 parts of codonopsis, 1.5 parts of stir-fried atractylodes, 1.5 parts of tangerine peel, 0.5 parts of roasted liquorice, and 0.5 parts of hawthorn.

[0064] Example 3: The feed additives in Example 3 include 2 parts of roasted Astragalus, 2 parts of Codonopsis, 3 parts of stir-fried Atractylodes, 4 parts of dried tangerine peel, 0.1 part of roasted Licorice, and 0.2 part of Hawthorn.

[0065] Except for the feed additives, other components of the above Examples 1-3 are the same.

[0066] Example 4: Compared with Example 3, the sports horse feed in Example 4 does not contain added micronutrients and complex amino acids.

[0067] The feed additives in Examples 1-4 above can be obtained by crushing the raw materials and then passing them through a sieve (e.g., 100 mesh), and the resulting powder can be used as the feed additive. When preparing the feed, the feed additive can be directly added to the prepared base feed and thoroughly mixed with the other components. In Examples 1-3 of the present invention, the powdered feed additive obtained after crushing is mixed with apple powder, carrot powder, micronutrients, and amino acids, then pressed into granules, and added to the other components and mixed evenly.

[0068] In the preferred embodiment of the present invention, the feed additive accounts for 12% of the total weight of the feed for sports horses. The following embodiment 3 is selected to feed sports horses and verify the effect of the feed additive on their post-exercise recovery ability:

[0069] (1) Grouping and model building

[0070] Twenty male horses of similar age, body shape (weighing approximately 600 kg) and endurance were selected from a horse farm in Huiji District, Zhengzhou City. After one week of adaptive feeding, they were randomly divided into four groups, with 5 horses in each group, namely, a model group, a test group, a positive control group and a negative control group. The model group was fed with a basal diet, the test group was fed with the sports horse feed of Example 3, the positive control group was fed with a special horse feed for Red Rum racing horses, and the negative control group was fed with the sports horse feed of Example 4.

[0071] (2) Experimental implementation

[0072] Each group undergoes systematic training every day based on the horse's function: 30 minutes of circling, followed by 40-60 minutes of flat riding training and 30 minutes of obstacle training, achieving medium to high intensity exercise.

[0073] Feeding method: Feed according to the normal method for sports horses, and feed with hay at the same time. The daily intake of hay per horse is 20kg, and the daily intake of feed per horse is 9kg, which are fed in 4 times.

[0074] (3) Test cycle

[0075] The trial lasted 30 days, with behavioral and physiological indicators collected daily. On the final day of the trial, blood was collected from the jugular vein 2 hours before, 0.5 hours after, and 24 hours after exercise training (immediately followed by cold compresses to prevent congestion and swelling). A portion of the blood was placed in an anticoagulant tube for routine blood tests, and a portion was centrifuged (3000 rpm) for the measurement of serum parameters (liver and kidney function and exercise biochemical parameters).

[0076] Among them, behavioral information indicators include food intake and feeding speed; daily physiological indicators include feces and urine, feces indicators specifically include feces morphology, color, frequency, discharge volume, etc., urine indicators include urine pH, etc.; routine blood tests specifically include red blood cells (RBC), white blood cells (WBC), neutrophils (NE); liver and kidney function specifically include alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (Cr), urea nitrogen (BUN); exercise biochemical information includes creatine kinase (CK) and lactate dehydrogenase (LDH).

[0077] (4) Test results

[0078] The fecal and urine physiological indicators of each group are shown in Table 1 below.

[0079]

[0080] Among them, the feces and urine of the horses in the experiment are represented by "A", "A" means no difference from usual, "-A" means a decrease from usual, and "+A" means an increase from usual.

[0081] The results of the behavioral information indicators are shown in Table 2 below.

[0082]

[0083]

[0084] Among them, the performance of feed intake and feeding speed is represented by "B", -B means a decrease compared to usual, B means the same as usual, +B means an increase compared to usual, and "numbers 1, 2, 3" are used to represent contrast.

[0085] Figure 1-3 The results of blood routine indexes of each group of horses 2 hours before exercise, 0.5 hours after exercise, and 24 hours after exercise are shown respectively; Figure 1 The results of the red blood cell (RBC) of each group of horses 2 hours before exercise, 0.5 hours after exercise and 24 hours after exercise are shown. Figure 2 The results of the white blood cell (WBC) of each group of horses 2 hours before exercise, 0.5 hours after exercise and 24 hours after exercise are shown. Figure 3 The results of neutrophil (NE) in each group of horses 2 hours before exercise, 0.5 hours after exercise, and 24 hours after exercise are shown.

[0086] Figure 4-7 The results of liver and kidney function indexes of each group of horses 2 hours before exercise, 0.5 hours after exercise and 24 hours after exercise are shown respectively; Figure 4 The results of alanine aminotransferase (ALT) of horses in each group 2 hours before exercise, 0.5 hours after exercise and 24 hours after exercise are shown. Figure 5 The results of aspartate aminotransferase (AST) of horses in each group at 2 hours before exercise, 0.5 hours after exercise, and 24 hours after exercise are shown. Figure 6 The results of creatinine (Cr) of each group of horses 2 hours before exercise, 0.5 hours after exercise and 24 hours after exercise are shown. Figure 7 The results of the urea nitrogen (BUN) of the horses in each group are shown 2 hours before exercise, 0.5 hours after exercise, and 24 hours after exercise.

[0087] Figure 8-9 The results of exercise biochemical information of each group of horses 2 hours before exercise, 0.5 hours after exercise and 24 hours after exercise are shown respectively; Figure 8The results of creatine kinase (CK) of horses in each group at 2 hours before exercise, 0.5 hours after exercise and 24 hours after exercise are shown. Figure 9 The results of lactate dehydrogenase (LDH) of horses in each group 2 hours before exercise, 0.5 hours after exercise, and 24 hours after exercise are shown.

[0088] (5) Result analysis

[0089] 5.1) As can be seen from Table 1, the feces and urine excretion of the model group during the feeding period was not much different from that of the usual period. The excretion and frequency of horse No. 4 were slightly lower than usual, which may be due to increased exercise and is a normal phenomenon of the body. The average excretion and frequency of horses in the experimental group were significantly higher than usual. This should be the effect of the feed additives added to the experimental group to strengthen the spleen and replenish qi and increase metabolite excretion. The excretion and frequency of the positive control group were not significantly different from usual. The average excretion frequency and excretion of the negative control group were higher than usual, indicating that the feed additives have a good effect of replenishing the middle and replenishing qi, which can enhance the circulation of qi and blood in horses and enhance the body's transportation and transformation capacity. Compared with the experimental group, the negative control group did not add trace elements and complex amino acids to the feed of the exercise horses, indicating that trace elements and complex amino acids can play a synergistic role with feed additives.

[0090] 5.2) As can be seen from Table 2, the feed intake and feeding speed of the model group during the feeding period were not much different from those at normal times; the average feed intake of the horses in the experimental group increased, which should be due to the improvement of the spleen and stomach transportation function, which increased the discharge and thus the feed intake; the feed intake and feeding speed of the positive control group both increased; the feed intake of the negative control group increased during the experimental period, but decreased slightly for the experimental group, indicating that the feed additives can still bring positive feedback effects to the horse body after the lack of trace elements and complex amino acids.

[0091] 5.3) From Figure 1-3It can be seen that there was no significant difference in the red blood cell count in the routine blood tests of each group before and after exercise. The increase in the number of individual red blood cells may be due to mild dehydration after exercise, which leads to an increase in the amount of red blood cells; the white blood cells and neutrophils in the routine blood tests of the model group were quite different before and after exercise. This may be the result of the stress response of the horse's body. After strenuous exercise, the horse's body triggers an "acute phase response", causing the bone marrow to release neutrophils to cope with metabolic stress and tissue damage. On the other hand, exercise may cause micro-tears in muscle fibers, releasing inflammatory cytokines to stimulate white blood cell proliferation and migration; the white blood cells and neutrophils in the routine blood tests of the experimental group were significantly lower than those of the model group. The reason may be that the added feed additives contain astragalus and codonopsis, which are natural "strengthening the body and nourishing the essence" ingredients. After exercise, the body produces a "stress response". The polysaccharide components in astragalus and codonopsis can activate the activity of immune cells and increase the body's anti-stress ability. Yeast selenium, as an organic selenium source, enhances antioxidant capacity, reduces the damage of free radicals to muscles and cells after exercise, and accelerates repair; vitamin E synergizes with selenium in antioxidants to protect cell membranes; magnesium sulfate participates in glycolysis and oxidative phosphorylation pathways, can relieve muscle spasms and promote energy metabolism.

[0092] 5.4) From Figure 4-7 As can be seen, alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (Cr), and blood urea nitrogen (BUN) in the model group increased significantly after exercise and began to gradually recover 24 hours after exercise. The increase in ALT and AST may be due to physiological muscle damage in horses after exercise. Strenuous exercise produces a large number of free radicals, which trigger lipid peroxidation and protein oxidative damage, leading to cell membrane rupture and enzyme release. On the other hand, metabolic stress increases energy demand during exercise, and the liver participates in glycogenolysis, fatty acid oxidation, and ammonia detoxification. The increased metabolic load may cause a temporary increase in liver cell membrane permeability. The significant increase in creatinine and blood urea nitrogen may be due to the accumulation of metabolic products caused by strenuous exercise: muscle activity accelerates metabolism, releasing creatinine into the blood; and protein breakdown increases during exercise (such as muscle energy supply), which increases the rate at which the liver converts amino acids to blood urea nitrogen.

[0093] The liver and kidney function indicators of the experimental group also increased significantly in the early stage after exercise, but recovered significantly faster than the model group and the positive control group after 24 hours. This should be related to the components of the feed additives, such as hawthorn, tangerine peel, and white atractylodes. Hawthorn has the effect of promoting protein decomposition and accelerating muscle repair, which can speed up the damage of horses after exercise, allowing them to quickly recover their physical strength and reach a normal state; white atractylodes and tangerine peel can ensure that feed is efficiently converted into energy and reduce the accumulation of metabolic waste. The potassium ions in potassium carbonate participate in the regulation of cell membrane potential, relieve muscle spasms and fatigue, can effectively replenish electrolytes, maintain acid-base balance and neuromuscular function, and prevent hypokalemia after exercise; sodium chloride can replenish sodium and chloride lost in sweat, and maintain the osmotic pressure and fluid balance of horses before and after exercise. B vitamin complexes and nicotinamide, as coenzymes (such as NAD + , FAD) participate in the tricarboxylic acid cycle and glucose metabolism, reducing lactic acid accumulation.

[0094] 5.5) From Figure 8-9 Serum creatine kinase (CK) and lactate dehydrogenase (LDH) levels were significantly elevated in the model group before and after exercise. Creatine kinase levels did not increase significantly after exercise, but increased significantly within 24 hours. This is because prolonged exercise causes muscles to rely on anaerobic glycolysis for energy, accelerating muscle fiber energy consumption and releasing CK from the cells into the blood. Alternatively, prolonged exercise depletes glycogen reserves, forcing muscles to break down protein for energy, increasing CK release. Furthermore, intense exercise can cause microtears in muscle fibers, increasing cell membrane permeability and releasing LDH from muscle cells into the blood, leading to elevated urea nitrogen. The post-exercise increases in CK and LDH in the experimental group were slower than those in the model group. This is likely due to the Chinese medicinal ingredients in the feed additives in the experimental group accelerating muscle repair after exercise and reducing muscle fiber damage. Calcium phosphate provides calcium and phosphorus to the horse's body. Calcium ions participate in myofilament sliding, and phosphorus is essential for ATP synthesis. Supplementing both calcium and phosphorus has a positive effect on regulating bone strength and muscle contraction function in horses. Vitamin D3 can promote calcium and phosphorus absorption and strengthen bones; vitamin K participates in blood coagulation and osteocalcin synthesis, and works synergistically with calcium hydrogen phosphate to accelerate the recovery of damaged muscles and increase bone strength.

[0095] 5.6) The experimental group's horse feed also contained B vitamins, complex amino acids, and a variety of micronutrients. These ingredients can accelerate recovery from sports injuries, enable horses to quickly regain their strength after exercise, efficiently utilize glycogen during exercise, improve immune stress resistance, and reduce oxidative damage.

[0096] The core efficacy of this feed additive is to tonify the spleen and replenish qi, elevate yang and lift deficient bodies. It synergizes with vitamins, trace elements, and complex amino acids to enhance energy metabolism. Astragalus polysaccharides and codonopsis saponins activate the AMPK pathway, improving mitochondrial function and promoting glycogen synthesis. B vitamins directly participate in ATP production, while magnesium and phosphorus assist in energy conversion. Their combined use can accelerate glycogen repletion after exercise and reduce fatigue.

[0097] The flavonoids and saponins in Astragalus scavenges free radicals, the Atractylodes polysaccharide enhances the activity of macrophages, and the yeast selenium and vitamin E provide antioxidant defense, which can dually reduce oxidative damage and lower inflammatory responses.

[0098] Vigorous exercise consumes Qi and damages body fluids. Glycyrrhizic acid regulates water and sodium metabolism, and works together with potassium carbonate and sodium chloride to maintain electrolyte balance, thereby preventing dehydration and improving "Qi deficiency".

[0099] Codonopsis promotes protein synthesis and synergizes with calcium hydrogen phosphate and vitamin D3 to strengthen bones. Calcium and phosphorus are directly involved in bone reconstruction, effectively reducing sports-induced bone micro-damage and accelerating bone and muscle repair.

[0100] Atractylodes macrocephala and licorice enhance gastrointestinal motility and mucosal barrier function, improving the bioavailability of trace elements (such as selenium and magnesium). Traditional Chinese medicine regulates intestinal flora, promoting the digestion and absorption of short-chain fatty acids while also promoting mineral absorption.

[0101] Trace elements, vitamins and complex amino acids quickly correct metabolic imbalances, and feed additives containing Chinese herbal ingredients regulate qi and blood as well as spleen and stomach function. They can also relieve gastrointestinal irritation that may be caused by excessive intake of a single nutrient. The two work together to achieve the concept of "treating both the symptoms and the root causes", which is very effective for horses undergoing long-term high-intensity training.

[0102] Although the present invention has been described above through embodiments, the above embodiments are only used to exemplify the possible implementation schemes of the present invention and are not used to limit the scope of protection of the present invention. Any equivalent substitutions or changes made by those skilled in the art in accordance with the present invention should also be covered by the scope of protection defined by the claims of the present invention.

Claims

1. A feed additive for improving the recovery ability of equine animals after exercise, characterized in that: The feed additives include formula No. I and formula No. II; wherein, formula No. I is composed of astragalus and codonopsis, and formula No. II is composed of atractylodes and tangerine peel.

2. The feed additive for improving the recovery ability of equines after exercise according to claim 1, characterized in that: The mass ratio of Formula No. Ⅰ to Formula No. Ⅱ is (1-4): (3-6).

3. The feed additive for improving the recovery ability of equines after exercise according to claim 2, characterized in that: The mass ratio of Astragalus and Codonopsis in Formula No. Ⅰ is (1-4): (1-4), and the mass ratio of Atractylodes and Citrus Reticulata in Formula No. Ⅱ is (1-2): (2-4).

4. The feed additive for improving the recovery ability of equines after exercise according to claim 1, characterized in that: Astragalus is roasted Astragalus, and Atractylodes is stir-fried Atractylodes.

5. The feed additive for improving the recovery ability of equines after exercise according to claim 1, characterized in that: The feed additive also includes formula III, which consists of hawthorn and liquorice.

6. The feed additive for improving the recovery ability of equines after exercise according to claim 5, characterized in that: The mass ratio of hawthorn and licorice in formula III is (1-5): (1-5).

7. The feed additive for improving the recovery ability of equines after exercise according to claim 5, characterized in that: The mass proportion of Formula III in feed additives is less than 15%.

8. Use of the feed additive for improving the recovery ability of equine animals after exercise according to any one of claims 1 to 7 in a diet for sports horses, characterized in that: Add the feed additive to the basic feed of sports horses at a ratio of 8%-20%.

9. Use of the feed additive for improving the recovery ability of equine animals after exercise in a diet for sports horses according to claim 8, characterized in that: The complex amino acids are added to the basic feed of sports horses at a ratio of 2% to 4%, wherein the complex amino acids are composed of lysine, methionine, threonine, tryptophan and glutamine.

10. Use of the feed additive for improving the recovery ability of equine animals after exercise in a diet for sports horses according to claim 8, characterized in that: Micronutrients are added to the basic feed of sports horses at a ratio of 0.5%-2%; wherein the micronutrients include minerals and vitamins, the minerals include at least one of yeast selenium, potassium carbonate, calcium hydrogen phosphate, sodium chloride and magnesium sulfate, and the vitamins include at least one of vitamin A, vitamin B, vitamin D3, vitamin K, vitamin E and niacinamide.

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