Hydrosol material as well as preparation method and application thereof

Abstract

The invention belongs to the field of biomedical materials and skin radiation protection, and discloses a hydrosol material and a preparation method and application thereof.The hydrosol material is prepared by taking chlorogenic acid and chitosan hydrochloride as main active ingredients through a scientific and reasonable proportion and process, can be used as a protective agent for infectious radiation skin diseases, and has the advantages of being environmentally friendly and free of toxic and side effects. The invention has the beneficial effects that the chitosan hydrochloride has remarkable bacteriostatic activity, and can form a hydrosol layer on the skin surface to block the adhesion and proliferation of external bacteria, so that bacterial infection in the skin radiation injury process can be prevented and treated; the chlorogenic acid has efficient free radical scavenging activity and can effectively scavenge toxic free radicals generated by low-energy ionizing radiation induction, so that the damage of the toxic free radicals to skin tissues is reduced, and the protection effect on the skin is achieved; the chlorogenic acid and chitosan hydrochloride hydrosol material can synergistically realize dual radiation protection integrating free radical scavenging and antibiosis.

Description

Technical Field

[0001] The present invention relates to the fields of biomedical materials and skin radiation protection, and in particular to a hydrosol material and a preparation method and application thereof. Background Art

[0002] Among the many radiation-related injuries, skin radiation damage is one of the most concerning. This is because the skin is the largest organ in the human body, surrounding the entire body and in direct contact with the surrounding environment. It is the first to be exposed to any harmful factors, making it the most common skin injury during radiation therapy and radiation accidents. If the skin receives too much radiation, such as when some seriously ill cancer patients receive excessive radiation doses, resulting in excessive radiation absorption by the skin; or when patients with superficial tumors inevitably experience severe skin reactions during radiation therapy; or when radiation workers encounter radiation accidents that result in excessive radiation exposure to their skin, they can experience irreversible skin atrophy, destruction of sebaceous and sweat glands, and permanent hair loss, leading to radiation necrosis and subsequent ulcer formation.

[0003] From a molecular biological perspective, radiation damage to the skin primarily results from the interaction of radiation with molecules or atoms (primarily water) in cells, causing these molecules or atoms to dissociate and generate a large number of toxic free radicals. These free radicals then attack cellular components, causing ultrastructural damage and ultimately inducing cell dysfunction and death. Therefore, most traditional commercial skin radioprotectants incorporate free radical scavengers (such as SOD and resveratrol) into their formulations. However, the free radical storm, an upstream event in the onset of damage, can trigger a series of cascading effects and secondary symptoms, and relying solely on free radical scavenging is often ineffective. Our extensive research has shown that free radical storms typically damage skin structure, making them highly susceptible to bacterial infections, further exacerbating radiation damage and hindering wound healing. After radiotherapy, 48% of patients developed severe acute radiation dermatitis, with Staphylococcus aureus detected in bacterial cultures. However, current commercial skin radioprotectants focus less on both free radical scavenging and bacterial infections during skin radiation damage. Therefore, it is expected in this field to design a new skin radioprotectant with better therapeutic effect, better safety performance, and dual radiation protection of free radical scavenging and antibacterial. Summary of the Invention

[0004] To solve the above problems, the present invention provides a hydrosol material and a preparation method and application thereof, which are achieved through the following technical solutions.

[0005] A hydrosol material comprises chlorogenic acid and chitosan hydrochloride, wherein the mass ratio of chlorogenic acid to chitosan hydrochloride is 1:(10-50).

[0006] As a further embodiment of the invention, the mass ratio of chlorogenic acid to chitosan hydrochloride is 1:35.

[0007] A method for preparing a hydrosol material. The hydrosol material is prepared by mixing chlorogenic acid and chitosan hydrochloride in water.

[0008] As a further solution of the invention, the following steps are included:

[0009] S1, preparing chlorogenic acid aqueous solution;

[0010] S2, mixing the chlorogenic acid aqueous solution with chitosan hydrochloride powder to obtain a chlorogenic acid@chitosan hydrochloride hydrosol material.

[0011] As a further embodiment of the present invention, in step S1, the chlorogenic acid aqueous solution is prepared as follows: adding chlorogenic acid powder into ultrapure water and dissolving the powder to obtain the chlorogenic acid aqueous solution;

[0012] The chlorogenic acid powder is dissolved by ultrasonic dissolution; the dissolution time is 2 to 10 minutes; and the concentration of the prepared chlorogenic acid aqueous solution is 0.5 to 2 mg / mL.

[0013] As a further embodiment of the invention, the dissolution time of the chlorogenic acid powder is 5 min, and the concentration of the prepared chlorogenic acid aqueous solution is 2 mg / mL.

[0014] As a further embodiment of the present invention, in step S2, the chlorogenic acid aqueous solution and chitosan hydrochloride powder are mixed and stirred at room temperature for 5 to 20 minutes.

[0015] As a further embodiment of the present invention, the mixing time of the chlorogenic acid aqueous solution and the chitosan hydrochloride powder is 10 minutes.

[0016] The invention discloses an application of a hydrosol material as a protective agent for infectious radiation skin diseases.

[0017] The beneficial effects of the present invention are that chitosan hydrochloride has significant antibacterial activity and can form a hydrosol layer on the skin surface, blocking the attachment and proliferation of external bacteria, thereby preventing and treating bacterial infections during skin radiation damage; chlorogenic acid has high-efficiency free radical scavenging activity and can effectively scavenge toxic free radicals induced by low-energy ionizing radiation, thereby reducing the damage of toxic free radicals to skin tissue and achieving a protective effect on the skin; chlorogenic acid@chitosan hydrochloride hydrosol material can synergistically achieve dual radiation protection that combines free radical scavenging and antibacterial properties. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solution of the present invention, the following is a brief introduction to the drawings required for use in the description of the specific implementation methods. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0019] Figure 1 :Photo of chlorogenic acid@chitosan hydrochloride hydrosol material;

[0020] Figure 2 : Rheological frequency-modulus curve of chlorogenic acid@chitosan hydrochloride hydrosol material;

[0021] Figure 3 : Rheological time-modulus curve of chlorogenic acid@chitosan hydrochloride hydrosol material;

[0022] Figure 4 :The scavenging effect of chlorogenic acid@chitosan hydrochloride hydrosol material on DPPH free radicals;

[0023] Figure 5 :Chlorogenic acid@chitosan hydrochloride hydrosol material to ABTS + · Free radical scavenging effect diagram;

[0024] Figure 6 :Chlorogenic acid@chitosan hydrochloride hydrosol material to·O 2- Diagram of the free radical scavenging effect;

[0025] Figure 7 :The scavenging effect of chlorogenic acid@chitosan hydrochloride hydrosol material on ·OH free radicals;

[0026] Figure 8 :Antibacterial effect diagram of chlorogenic acid@chitosan hydrochloride hydrosol material;

[0027] Figure 9 :Bacterial survival rate after the action of chlorogenic acid@chitosan hydrochloride hydrosol material;

[0028] Figure 10 :The actual effect pictures of mouse skin after different treatments;

[0029] Figure 11 : Pathological results of mouse skin tissues after different treatments. DETAILED DESCRIPTION

[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0031] like Figure 1-11 As shown, a hydrosol material includes chlorogenic acid and chitosan hydrochloride, and the mass ratio of chlorogenic acid to chitosan hydrochloride is 1:(10-50).

[0032] Preferably, the mass ratio of chlorogenic acid to chitosan hydrochloride is 1:35.

[0033] The invention discloses a method for preparing a hydrosol material. The hydrosol material is prepared by mixing chlorogenic acid and chitosan hydrochloride in water.

[0034] As a further solution of the invention, the following steps are included:

[0035] S1, preparing chlorogenic acid aqueous solution;

[0036] S2, mixing the chlorogenic acid aqueous solution with chitosan hydrochloride powder to obtain a chlorogenic acid@chitosan hydrochloride hydrosol material.

[0037] As a further embodiment of the present invention, in step S1, the chlorogenic acid aqueous solution is prepared as follows: adding chlorogenic acid powder to ultrapure water and dissolving it to obtain the chlorogenic acid aqueous solution;

[0038] The chlorogenic acid powder is dissolved by ultrasonic dissolution; the dissolution time is 2 to 10 minutes; and the concentration of the prepared chlorogenic acid aqueous solution is 0.5 to 2 mg / mL.

[0039] As a further embodiment of the invention, the dissolution time of the chlorogenic acid powder is 5 min, and the concentration of the prepared chlorogenic acid aqueous solution is 2 mg / mL.

[0040] Preferably, in step S2, the chlorogenic acid aqueous solution and chitosan hydrochloride powder are mixed and stirred at room temperature for 5 to 20 minutes.

[0041] Preferably, the mixing time of the chlorogenic acid aqueous solution and the chitosan hydrochloride powder is 10 minutes.

[0042] The invention discloses an application of a hydrosol material as a protective agent for infectious radiation skin diseases.

[0043] Chlorogenic acid possesses excellent free radical scavenging capabilities, effectively scavenging toxic free radicals induced by radiation, thereby reducing oxidative damage to the skin. This innovative formulation utilizes chlorogenic acid, derived from a natural plant source, as its core functional ingredient. This polyphenolic compound exhibits exceptional free radical scavenging capabilities through its unique catechol and carboxylic acid groups. Its antioxidant mechanism involves a dual pathway: direct neutralization of reactive oxygen species through hydrogen atom transfer, and sustained electron buffering through single electron transfer. This dynamic scavenging mode rapidly captures a variety of toxic free radicals induced by radiation. It is particularly noteworthy that chlorogenic acid, as an FDA-certified food-grade substance, exhibits excellent biocompatibility and metabolic safety. Its water solubility not only enhances skin penetration but also forms a homogeneous and stable complex with chitosan hydrochloride hydrosol, creating a dual radiation protection barrier for the skin.

[0044] Chitosan hydrochloride has significant antibacterial activity and can form a hydrosol layer on the skin surface, blocking the attachment and proliferation of external bacteria. Therefore, it can prevent and treat bacterial infections during skin radiation damage.

[0045] This application innovatively develops a chlorogenic acid@chitosan hydrochloride hydrosol material as a protective agent for infectious radiation-induced skin diseases. Through the synergistic effect of chlorogenic acid and chitosan hydrochloride, a multiple protection mechanism is constructed: the efficient free radical scavenging ability of chlorogenic acid can neutralize the reactive oxygen free radicals induced by radiation, while chitosan hydrochloride exerts its antibacterial effect; when administered after irradiation, the system continues to exert its repair function and promotes the recovery of damaged skin.

[0046] Compared with existing protective agents, the protective agent of this application provides all-round skin protection for radiotherapy patients and workers who are exposed to radiation for a long time. The successful development of this product not only fills the shortcomings of existing protective technology in terms of sustained protection effect, but also provides a safe and efficient new solution for clinical skin radiation protection, which has important clinical application value and social benefits.

[0047] It is particularly worth noting that the chitosan hydrochloride used in this protective agent is a clinically proven medical-grade wound healing material with excellent biocompatibility. Chlorogenic acid, as a natural polyphenol compound, has been proven safe in many fields, thus ensuring the safety of the product.

[0048] The specific implementation method of the present invention is as follows:

[0049] (1) Preparation of chlorogenic acid@chitosan hydrochloride hydrosol material

[0050] like Figure 1As shown, 6 mg of chlorogenic acid powder was added to 3.0 mL of ultrapure water and dissolved by ultrasound for 5 minutes to obtain a 2 mg / mL chlorogenic acid aqueous solution; then 1 mL of the prepared chlorogenic acid aqueous solution was mixed with 70 mg of chitosan hydrochloride powder (the mass ratio of chlorogenic acid to chitosan hydrochloride powder was 1:35), and stirred at room temperature to form a uniform chlorogenic acid@chitosan hydrochloride hydrosol material.

[0051] (2) Determination of rheological properties of hydrosol materials

[0052] Testing Principle: A hydrosol is a sol using water as the dispersion medium. The dispersed particles can be single macromolecules or aggregates of many molecules. Rheology is the study of the deformation and flow of materials. For soft materials such as hydrosols, rheological testing characterizes the material's viscoelastic and rheological properties by applying controlled stress or strain and analyzing its dynamic mechanical response. Testing aims to evaluate the deformation and flow behavior of hydrosols under different stress conditions to understand their physical and rheological properties.

[0053] The formation and stability of chlorogenic acid@chitosan hydrochloride hydrosol were evaluated by rheological experiments. The results showed that the storage modulus and loss modulus of the material were related to the frequency. At all tested frequencies (1-100 Hz), the loss modulus was slightly larger than the storage modulus ( Figure 2 ), indicating that a hydrosol material is formed, and the storage modulus and loss modulus remain unchanged during the entire test time ( Figure 3 ), indicating that the hydrosol has good mechanical stability.

[0054] (3) Chlorogenic acid scavenging test of DPPH free radicals

[0055] The free radical scavenging activity of chlorogenic acid was determined using the DPPH assay. The specific experimental steps are as follows: 100 μmol / L DPPH (solvent: anhydrous ethanol) and a series of aqueous chlorogenic acid solutions of varying concentrations (6.25 μg / mL, 12.5 μg / mL, 25 μg / mL, 50 μg / mL, and 100 μg / mL) were prepared. The chlorogenic acid solutions of varying concentrations were mixed evenly with the DPPH solution (at a 1:1 volume ratio). The mixture was incubated at room temperature in the dark for 30 minutes, and the absorbance at 517 nm was measured using a UV spectrophotometer.

[0056] Test principle: DPPH is a stable free radical. Its solution is dark purple and has a characteristic absorption peak at 517nm. When the free radical scavenger reacts with DPPH, it can provide hydrogen atoms or electrons to neutralize the free radicals, resulting in a decrease in the absorbance value of the solution at 517nm. By measuring the change in absorbance, the free radical scavenging ability of chlorogenic acid can be directly reflected. Finally, the free radical scavenging test results of the chlorogenic acid aqueous solution are obtained, such as Figure 4 As shown in the figure, with the increase of chlorogenic acid concentration, the absorbance value of the solution at 517 nm decreased, indicating that the free radical scavenging ability of chlorogenic acid is positively correlated with its concentration.

[0057] (4) Chlorogenic acid on ABTS + Free radical scavenging test

[0058] The effect of chlorogenic acid on ABTS was determined by the following method + Free radical scavenging activity: ABTS assay was used. The specific experimental steps were as follows: 7 mmol / L ABTS solution (solvent: ultrapure water) and 2.45 mmol / L potassium persulfate solution were prepared. The ABTS solution and potassium persulfate solution were mixed by volume. After reacting at room temperature in the dark for 16 hours, the reaction solution was diluted with PBS to an absorbance of 1.5 at 734 nm to obtain ABTS. + Free radical working solution, and a series of chlorogenic acid aqueous solutions with different concentrations (6.25μg / mL, 12.5μg / mL, 25μg / mL). + Mix the free radical working solution evenly (mix according to a volume ratio of 1:1), react at room temperature in the dark for 10 minutes, and then measure the absorbance at 734 nm using a UV spectrophotometer.

[0059] Test principle: ABTS generates blue-green ABTS under potassium persulfate oxidation + Free radicals, with a characteristic absorption peak at 734nm. Chlorogenic acid can neutralize ABTS by donating hydrogen atoms or electrons + ·, resulting in a decrease in absorbance, ABTS + The degree of scavenging is measured by the degree of reduction of the characteristic absorption value, and finally the test results of scavenging free radicals by chlorogenic acid aqueous solution are obtained, such as Figure 5 As shown in the figure, with the increase of chlorogenic acid concentration, the absorbance value of the solution at 734 nm decreased, indicating that the free radical scavenging ability of chlorogenic acid is positively correlated with its concentration.

[0060] (5) Chlorogenic acid to ·O 2- Free radical scavenging test

[0061] The free radical scavenging activity of chlorogenic acid was determined by the following method: 2-The specific experimental steps for the detection method are as follows: 0.25 mmol / L NBT solution, 1 mmol / L NADH solution, and 15 μmol / L PMS (all solvents are ultrapure water) are prepared, along with a series of chlorogenic acid aqueous solutions of different concentrations (0 μg / mL, 250 μg / mL, and 500 μg / mL). PBS buffer (300 μL), NADH solution (150 μL, 1 mmol / L), NBT solution (100 μL, 0.25 mmol / L), and chlorogenic acid test solution (150 μL) are added to a quartz cuvette. Finally, PMS working solution (150 μL, 15 μmol / L) is added. The mixture is immediately mixed for 5 seconds to initiate the reaction, and the absorbance at 560 nm is monitored in real time in a UV spectrophotometer for 10 minutes.

[0062] Test principle: 2- Produced by the NADH / PMS system through redox reaction, the generated reduced PMS - Transfer electrons to dissolved oxygen to generate O 2- It reacts with NBT to generate a blue product with a characteristic absorption peak at 560nm, which can be used to determine the presence of O 2- The changes in the absorbance of the system at 560 nm were monitored in real time on a UV spectrophotometer for 10 min. Chlorogenic acid cleared ·O 2- Inhibits the formation of blue product, resulting in a decrease in absorbance. ·O 2- The degree of removal is measured by the degree of reduction of the characteristic absorption value. 2- Free radical scavenging test results, such as Figure 6 As shown in the figure, with the increase of chlorogenic acid concentration, the absorbance value of the solution at 560nm decreased, indicating that the free radical scavenging ability of chlorogenic acid is positively correlated with its concentration.

[0063] (6) Chlorogenic acid scavenging test of OH free radicals

[0064] The free radical scavenging activity of chlorogenic acid was determined using the ·OH assay. The following experimental steps were used: 1 mmol / L TMB solution (solvent: DMSO), 80 mmol / L H₂O₂ (solvents: ultrapure water), HAc-NaAc buffer (pH = 4.0), 4 mmol / LFeSO₄ (solvent: HAc-NaAc buffer, pH = 4.0), and a series of chlorogenic acid aqueous solutions at different concentrations (100 μg / mL and 200 μg / mL) were prepared. TMB solution (200 μL), H₂O₂ solution (200 μL), chlorogenic acid aqueous solution (200 μL), and FeSO₄ solution (200 μL) were added to a quartz cuvette, mixed immediately, and incubated in the dark for 5 minutes. The characteristic UV absorption peak (652 nm) was then measured.

[0065] Test principle: OH is reacted by the classic Fenton reaction (Fe 2+ The generated ·OH radical can oxidize TMB to a blue-green product with a characteristic absorption peak at 652nm. Chlorogenic acid inhibits TMB oxidation by scavenging ·OH radicals, resulting in a decrease in absorbance. The degree of ·OH radical scavenging is measured by the degree of decrease in the characteristic absorption value. The final chlorogenic acid aqueous solution scavenges ·OH radicals, as shown in the following figure: Figure 7 As shown in the figure, with the increase of chlorogenic acid concentration, the absorbance value of the solution at 652 nm decreased, indicating that the free radical scavenging ability of chlorogenic acid is positively correlated with its concentration.

[0066] The results of the above four free radical scavenging experiments showed that chlorogenic acid aqueous solution can effectively scavenge various types of free radicals and has broad-spectrum free radical scavenging properties, so it has the potential to be used as a radiation protection agent.

[0067] (7) Antibacterial effect test of chlorogenic acid@chitosan hydrochloride hydrosol material

[0068] The plate count method was used to detect the number of bacterial colonies to evaluate the antibacterial activity of chlorogenic acid@chitosan hydrochloride hydrosol. The experiment was divided into the following groups: (1) Control group; (2) Chlorogenic acid group; (3) Chitosan hydrochloride group; (4) Chlorogenic acid@chitosan hydrochloride hydrosol group. Staphylococcus aureus cultured overnight was diluted to a concentration of 1.0×10 6 CFU / mL, except for the addition of different drugs (normal saline was added to the control group), all other conditions were the same and incubated for 4 hours. After incubation, 100 μL of bacterial solution from each well was inoculated on an agar plate and incubated at 37°C overnight. Finally, the growth picture of the colony was obtained ( Figure 8 ), count, calculate bacterial survival rate, and analyze its antibacterial effect ( Figure 9 ).

[0069] Staphylococcus aureus is the main bacteria that causes infectious radiation-induced skin diseases. Experimental results show that chlorogenic acid @ chitosan hydrochloride hydrosol has excellent antibacterial effect against drug-resistant Staphylococcus aureus, providing support for the antibacterial process of chlorogenic acid @ chitosan hydrochloride hydrosol in the subsequent treatment of infectious radiation-induced skin diseases.

[0070] (8) Test of the protective effect of chlorogenic acid@chitosan hydrochloride hydrosol on infectious radiation-induced skin damage.

[0071] First, 8-week-old healthy ICR male mice were purchased (Chengdu Dashuo Experimental Animal Co., Ltd.) and randomly divided into the following groups: (i) pure X-ray irradiation group; (ii) commercial skin radiation protection ointment group + X-ray irradiation group; (iii) chlorogenic acid + X-ray irradiation group; (iv) chitosan hydrosol + X-ray irradiation group; (v) chlorogenic acid @ chitosan hydrochloride hydrosol + X-ray irradiation group. Before the experiment, the legs of the mice were depilated. During the experiment, the mice were anesthetized first, and then the corresponding materials of each group were applied to the back of the mice according to the requirements of each group. The commercial skin radiation protection ointment used was BIAFINE Biafine Repair Cream (Johnson & Johnson Santé Beauté France, which has been indicated to have no antibacterial function). It was applied evenly and allowed to absorb. About half an hour after the application of the drug, the back of the mouse was irradiated with soft X-rays for 2 minutes. After irradiation, 20 μL of 10 8 The mice were infected with Staphylococcus aureus at a concentration of 100 CFU / mL, and the changes in the back skin of the mice were observed and photographed every day. The mice were killed on the 9th day, and the mouse skin was removed for histopathological analysis.

[0072] The actual effect of mouse skin radiation protection is as follows Figure 10 As shown, edema and scald-like blisters were clearly observed in both the pure X-ray irradiation group and the commercial skin radiation protection ointment + X-ray irradiation group on day 6, while no obvious wounds were observed in the other groups. By day 9, the pure X-ray irradiation group had developed a certain degree of epidermal erosion, with abnormal wrinkles caused by radiation-induced skin deformation. The commercial skin radiation protection ointment + X-ray irradiation group also had erosions on the legs. Mild pathological lesions were observed in the chlorogenic acid + X-ray irradiation group and the pure chitosan hydrosol dressing + X-ray irradiation group, but these were already in the recovery phase. The chlorogenic acid @ chitosan hydrochloride hydrosol dressing + X-ray irradiation group had the least damage. Therefore, from this macroscopic perspective, the chlorogenic acid @ chitosan hydrochloride hydrosol dressing has a very excellent radiation protection effect, and its effect is better than that of the commercial skin radiation protection ointment without antibacterial function. Therefore, the chlorogenic acid@chitosan hydrochloride hydrosol dressing with antibacterial effect and free radical scavenging ability has great potential to be developed as a skin radiation protectant. Figure 11 As shown, Figure 11 and Figure 10 The results of the actual pictures are very consistent, which further proves from a microscopic perspective that the chlorogenic acid @ chitosan hydrochloride hydrosol dressing has a very excellent skin radiation protection effect on the skin.

[0073] The preferred embodiments of the present invention disclosed above are intended only to help illustrate the present invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the present invention to specific embodiments. Obviously, numerous modifications and variations are possible based on the contents of this specification. These embodiments are selected and described in detail in this specification to better explain the principles and practical applications of the present invention, thereby enabling those skilled in the art to better understand and utilize the present invention.

Claims

1. A hydrosol material, characterized in that: The hydrosol material includes chlorogenic acid and chitosan hydrochloride, and the mass ratio of chlorogenic acid to chitosan hydrochloride is 1:(10-50).

2. A hydrosol material according to claim 1, characterized in that: The mass ratio of chlorogenic acid to chitosan hydrochloride is 1:

35.

3. A method for preparing the hydrosol material according to claim 1, characterized in that: The hydrosol material is prepared by mixing chlorogenic acid and chitosan hydrochloride in water.

4. The method for preparing a hydrosol material according to claim 3, wherein: The following steps are involved: S1, preparing chlorogenic acid aqueous solution; S2, mixing the chlorogenic acid aqueous solution with chitosan hydrochloride powder to obtain a chlorogenic acid@chitosan hydrochloride hydrosol material.

5. The method for preparing a hydrosol material according to claim 4, wherein: In step S1, the chlorogenic acid aqueous solution is prepared as follows: chlorogenic acid powder is added to ultrapure water and dissolved to obtain the chlorogenic acid aqueous solution; The chlorogenic acid powder is dissolved by ultrasonic dissolution; the dissolution time is 2 to 10 minutes; and the concentration of the prepared chlorogenic acid aqueous solution is 0.5 to 2 mg / mL.

6. The method for preparing a hydrosol material according to claim 5, characterized in that: The dissolution time of the chlorogenic acid powder is 5 minutes, and the concentration of the prepared chlorogenic acid aqueous solution is 2 mg / mL.

7. The method for preparing a hydrosol material according to claim 4, wherein: In the step S2, the chlorogenic acid aqueous solution and the chitosan hydrochloride powder are mixed and stirred at room temperature for 5 to 20 minutes.

8. The method for preparing a hydrosol material according to claim 7, wherein: The mixing time of the chlorogenic acid aqueous solution and chitosan hydrochloride powder is 10 minutes.

9. Use of a hydrosol material prepared by the preparation method according to any one of claims 3 to 8, characterized in that: Used as a protective agent for infectious radiation skin diseases.

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