Batch sterilization device containing integrated inoculating loop
By designing a cylindrical sterilization device with adjustable height and a manufacturing method for an integrated inoculation loop, the problems of poor adaptability and easy contamination of existing inoculation loop sterilization devices are solved, efficient and low-cost batch sterilization operations are achieved, and the accuracy of experimental results is ensured.
Patent Information
- Application Number
- CN202510913814.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-03
- Publication Date
- 2025-09-23
AI Technical Summary
Existing sterilization devices for inoculation loops have problems such as poor adaptability, easy contamination, and cumbersome operation, which affect experimental efficiency and result accuracy, especially when used in large quantities.
A cylindrical sterilization device consisting of a lid, an upper part and a lower part was designed. The height adjustment was achieved through threaded connections, and steps and partitions were set inside to accommodate inoculation loops of different lengths. An integrated inoculation loop manufacturing method was adopted, and auxiliary equipment such as clamps, hooks and rotators were used to quickly manufacture the integrated inoculation loop.
It realizes flexible adaptation and batch sterilization of inoculation loops, reduces the risk of contamination, improves operational convenience and experimental efficiency, and ensures the accuracy and reliability of experimental results.
Smart Images
Figure CN120678974A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of microbial culture, and in particular relates to a batch sterilization device containing an integrated inoculating loop. Background Art
[0002] Inoculating loops are tools used in microbiology experiments to pick up microbial samples and transfer them from one culture medium to another to achieve microbial growth and purification. In this process, inoculating loops play a vital role as a medium, ensuring that microbial samples can be transferred accurately and without contamination.
[0003] Currently, inoculating loops are typically sterilized by flame ignition, but specialized designs or devices capable of batch sterilization are relatively scarce. While simple stainless steel barrels are widely used for batch sterilization of inoculating loops, they have exhibited significant deficiencies in practice. The primary issue is their fixed height and lack of telescopic adjustment, making them difficult to accommodate inoculating loops of varying lengths and inconvenient for batch use. Furthermore, when removing an inoculating loop from the barrel, fingers (or gloves) inevitably come into direct contact with the handle (tail) of the loop, causing microorganisms carried by the fingers to transfer and adhere to the surface of the tail. When the operator grasps the tail of the inoculating loop to remove it, the natural rotation, tilting, or movement of the hand can easily cause the metal ring to inadvertently contact the similarly contaminated handles of other inoculating loops in the barrel. This contact allows contaminants from the handles of other inoculating loops in the barrel to directly transfer to the supposedly sterile metal ring. Once this contaminated ring is used to extract a sample or contact culture medium, it can introduce foreign microorganisms into the sterile environment or sample, seriously compromising the accuracy and reliability of experimental data. Therefore, the design defect of this fixed stainless steel barrel becomes a hidden danger in laboratory sterile operations.
[0004] Existing inoculation loops are usually made of metal or plastic and have different characteristics and applicable scenarios. Plastic inoculation loops are usually designed for disposable use. Although convenient, they are relatively expensive. For laboratories that perform inoculation operations frequently and in large quantities, this will undoubtedly increase the overall cost of the experiment. In contrast, metal inoculation loops can be reused, but they must be flame-burned after each use to ensure that they are in a sterile state. This step is not only cumbersome and time-consuming, but also greatly reduces work efficiency. Especially in situations where large-scale inoculation is required, the sterilization process of metal inoculation loops has become a key factor restricting experimental efficiency. In addition, the price of metal inoculation loops is also relatively high. In the context of laboratories pursuing efficiency and cost control, their use is subject to a certain degree of restriction. Summary of the Invention
[0005] In view of the above shortcomings, the object of the present invention is to provide a batch sterilization device containing an integrated inoculating loop.
[0006] To achieve this purpose, the technical solution adopted in the present invention is: The batch sterilization device is cylindrical in shape as a whole and includes a cover, an upper part, a lower part and an integrated inoculation loop; The cover is cylindrical, with one end open and the other end closed, and the tail end of the opening shrinks toward the center of the cylinder to form a slot; the slot is used for connection or fixation with other parts; The upper part is a cylindrical barrel structure, one end of the upper part is open and provided with an upper end thread, the other end of the upper part is open, and the outer diameter of the ring of the opening section is equal to or slightly smaller than the inner diameter of the ring of the groove section, so as to ensure that the opening and the groove can be tightly buckled, the cover is buckled to the upper part through the groove, and the cover and the upper part can be easily disassembled and tightly assembled; The lower part is a cylindrical barrel structure, one end of the lower part is open and provided with a lower end thread, the other end of the lower part is closed, and at least one step is provided at the closed position, the step is connected to the partition, and the partition divides the internal space of the lower part into at least two chambers, the outer diameter of the circular ring of the lower end thread opening is equal to or slightly smaller than the inner diameter of the circular ring of the upper end thread section, so as to ensure that the upper end thread and the lower end thread can be tightly screwed together; The upper end thread of the upper part is rotatably connected to the lower end thread of the lower part. By rotating the thread, the relative rotation of the upper and lower parts is converted into vertical displacement of the cylinder, so as to adjust the height of the batch sterilization device and obtain the maximum height and minimum height of the batch sterilization device. The integrated inoculation loop includes an elastic head, a handheld rod, and a clamping and fixing portion; the elastic head includes a circle and a winding rod, and the handheld rod includes a slender rod and a tail end; the height of the integrated inoculation loop is less than the maximum height of the batch sterilization device, and the height of the integrated inoculation loop is greater than the minimum height of the batch sterilization device; The integrated inoculation ring is made of a metal wire through bending, winding and other steps; the diameter of the metal wire is preferably selected from 0.3mm to 1mm, and the material of the metal wire is preferably selected from stainless steel or other possible materials; During sterilization, the integrated inoculating loop is placed inside the batch sterilization device with the circle facing downward and the tail facing upward. When the batch sterilization device needs to accommodate multiple integrated inoculating loops, the integrated inoculating loops are placed in the multiple chambers respectively. The method for making the integrated inoculating loop requires the use of auxiliary equipment such as a clamp, a hook, and a rotator. The hook is made by bending a metal rod, and the diameter of the hook is preferably selected from 1 mm to 5 mm. The production method comprises the following steps: The metal wire is bent after being overlapped end to end, and a single-layer bending loop is formed at the bending position of the metal wire; the metal wire is bent a second time, so that the end and end of the metal wire are inserted into the single-layer bending loop and fixed with a clamp; a double-layer bending loop is formed at the bending position; Secure the straight shank of the hook with a swivel; Hanging the hook in the single-layer bending circle, applying forces in opposite directions to the clamper and the rotator to straighten the wire as much as possible; The rotator is started to drive the hook to rotate, so that the metal wire is rotated and wound to form a winding rod. The length of the winding rod is preferably selected from 5 cm to 10 cm, and the number of rotation and winding of the winding rod is preferably selected from 5 to 15 turns per 1 cm length. The rotator is stopped after the metal wire is rotated and wound to a suitable number of turns. The wound metal wire is separated from the hook, and the contact portion of the metal wire and the hook forms a circle, the inner diameter of the circle being equal to the diameter of the hook, and the inner diameter of the circle is preferably selected from 1 mm to 5 mm; Hanging the hook in the double-layer bending circle, applying forces in opposite directions to the clamper and the rotator to straighten the wire as much as possible; The rotator is started to drive the hook to rotate, thereby causing the metal wire to rotate and wind to form a slender rod. The length of the slender rod is preferably selected from 10 cm to 30 cm, and the number of rotation and winding of the slender rod is preferably selected from 5 to 10 turns per 1 cm of length. The rotator is stopped after the metal wire has been rotated and wound to a suitable number of turns. The wound metal wire is separated from the hook, and the contact portion of the metal wire and the hook forms a tail loop; the clamp is released, and the portion of the metal wire fixed by the clamp forms a clamping and fixing portion; the head and tail ends of the metal wire extend and protrude outside the clamping and fixing portion; The length of the first and last ends of the metal wire is cut with a clamp, and force is applied to change its shape so that the first and last ends of the metal wire are tightly attached to the clamping and fixing parts; the shape of the tail ring is modified with a clamp to form the tail end.
[0007] Compared with the prior art, the present invention has the following beneficial effects: Relevant personnel can use non-professional common auxiliary equipment such as clamps, hooks, and rotators to quickly and conveniently batch-produce the integrated inoculating loops in a low-cost manner. The batch sterilization device can be used for batch sterilization operations of the integrated inoculating loops.
[0008] The sterilization device successfully converts the relative rotation of the upper and lower parts into vertical displacement of the cylinder through the precise rotational coordination of the upper and lower threads, thereby achieving flexible telescopic adjustment of the device's height. This design enables the sterilization device to adapt to inoculation loops of different lengths, greatly improving its practicality and flexibility. When it is necessary to remove the inoculation loop, the operator can easily lower the height of the sterilization device and extend the inoculation loop's handle from the sterilization device, greatly facilitating the removal of the inoculation loop while also reducing the risk of contamination caused by improper operation.
[0009] In addition, the steps and the partition structure provided inside the sterilization device separate the inoculation rings installed in the sterilization device into several areas, and the inoculation rings in each area have different heights. This design not only enables the integrated inoculation rings to be used in batches after sterilization, thereby improving work efficiency, but also further reduces the possibility of microorganisms on the operator's fingers being transferred to unused sterile inoculation rings through touch, thereby effectively reducing cross-contamination and ensuring the accuracy and reliability of subsequent experimental results. BRIEF DESCRIPTION OF THE DRAWINGS
[0010] Figure 1 This is a schematic diagram of the bending shape of the metal wire when making an integrated inoculating loop; Figure 2 This is a schematic diagram when making an elastic head; Figure 3 This is a schematic diagram after the elastic head is completed; Figure 4 This is a schematic diagram when making a handheld rod; Figure 5 This is a schematic diagram after the handheld rod is completed; Figure 6 This is a schematic diagram of the structure of the integrated inoculation loop after it is completed; Figure 7 It is a structural diagram of the sterilization device; In the picture: 1-elastic head, 11-circle, 12-winding rod, 2-hand-held rod, 21-tail ring, 22-slender rod, 23-tail end, 3-hook, 4-gripper, 5-rotator, 6—sterilization device, 61—cover, 62—slot, 63—upper part, 64—upper end thread, 65—lower end thread, 66—lower part, 67—step, 68—partition, 69—chamber, 7—Clamping and fixing place, 8—metal wire, 81—single-layer bending ring, 82—double-layer bending ring, 83—the beginning and end of the metal wire. DETAILED DESCRIPTION
[0011] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the embodiments described are only used to explain the present invention and are not intended to limit the present invention. Any simplification or equivalent changes made based on the technical solution of the present invention fall within the scope of protection of the present invention. Example
[0012] The present invention discloses a batch sterilization device comprising an integrated inoculation loop. When the present invention is applied to the inoculation of Lactobacillus plantarum (strain number ATCC8014), the following technical solutions are adopted to achieve the beneficial effects of the present invention: A batch sterilization device with an integrated inoculating loop, wherein the batch sterilization device 6 is cylindrical in shape and includes a cover 61, an upper portion 63, a lower portion 66 and an integrated inoculating loop; The cover 61 is cylindrical, with one end open and the other end closed. The tail end of the open portion contracts toward the center of the cylinder to form a slot 62. The slot 62 is used for connection or fixation with other parts. The upper portion 63 is a cylindrical tube-shaped structure. One end of the upper portion 63 is open and provided with an upper end thread 64. The other end of the upper portion 63 is open, and the outer diameter of the circular ring of the open section is equal to or slightly smaller than the inner diameter of the circular ring of the section of the slot 62, so as to ensure that the opening and the slot 62 can be tightly fastened. The cover 61 is fastened to the upper portion 63 through the slot 62, and the cover 61 and the upper portion 63 can be easily disassembled and tightly assembled. The lower portion 66 is a cylindrical, canister-shaped structure. One end of the lower portion 66 is open and provided with a lower thread 65. The other end of the lower portion 66 is closed and provided with at least one step 67 at the closed position. The step 67 is connected to a partition 68. The partition 68 divides the internal space of the lower portion 66 into at least two chambers 69. The outer diameter of the cut surface of the lower thread 65 is equal to or slightly smaller than the inner diameter of the cut surface of the upper thread 64, so as to ensure that the upper thread 64 and the lower thread 65 can be tightly screwed together. The upper end thread 64 of the upper part 63 is rotationally connected to the lower end thread 65 of the lower part 66. Through the rotation of the thread, the relative rotation of the upper part 63 and the lower part 66 is converted into displacement in the vertical direction of the cylinder, so as to realize the adjustment of the height of the batch sterilization device and obtain the maximum height and minimum height of the batch sterilization device.
[0013] The integrated inoculation loop includes an elastic head 1, a handheld rod 2, and a clamping and fixing portion 7; the elastic head 1 includes a circle 11 and a winding rod 12, and the handheld rod 2 includes a slender rod 22 and a tail end 23; the height of the integrated inoculation loop is less than the maximum height of the batch sterilization device, and the height of the integrated inoculation loop is greater than the minimum height of the batch sterilization device; The integrated inoculation ring is made of a metal wire 8 by bending, winding, and other steps; the diameter of the metal wire 8 is preferably selected from 0.3 mm to 1 mm, and the material of the metal wire 8 is preferably selected from stainless steel or other possible materials; During sterilization, the integrated inoculating loop is placed inside the batch sterilization device with the circle 11 facing downward and the tail end 23 facing upward. When the batch sterilization device needs to accommodate multiple integrated inoculating loops, the integrated inoculating loops are placed in multiple chambers 69 respectively. The method for making the integrated inoculating loop requires the use of auxiliary equipment such as a clamp 4, a hook 3, and a rotator 5. The hook 3 is made by bending a metal rod, and the diameter of the hook 3 is preferably selected from 1 mm to 5 mm. The production method comprises the following steps: The metal wire 8 is bent after being overlapped end to end, and a single-layer bending loop 81 is formed at the bending portion of the metal wire 8. The metal wire 8 is bent a second time, so that the end 83 of the metal wire is inserted into the single-layer bending loop 81 and fixed with the clamp 4. A double-layer bending loop 82 is formed at the bending portion. Fix the straight handle portion of the hook 3 with a rotator 5; Hang the hook 3 in the single-layer bending ring 81, and apply forces in opposite directions to the clamper 4 and the rotator 5 to straighten the metal wire 8 as much as possible; The rotator 5 is started to drive the hook 3 to rotate, so that the metal wire 8 is rotated and wound to form a winding rod 12. The length of the winding rod 12 is preferably selected from 5 cm to 10 cm, and the number of rotation and winding of the winding rod 12 is preferably selected from 5 to 15 turns per 1 cm of length. The rotator 5 is stopped after the metal wire 8 is rotated and wound to a suitable number of turns. The wound metal wire 8 is separated from the hook 3. The contact portion of the metal wire 8 and the hook 3 forms a circle 11. The inner diameter of the circle 11 is equal to the diameter of the hook 3. Therefore, the inner diameter of the circle 11 is preferably selected from 1 mm to 5 mm. Hang the hook 3 in the double-layer bending ring 82, and apply forces in opposite directions to the clamper 4 and the rotator 5 to straighten the wire 8 as much as possible; The rotator 5 is started to drive the hook 3 to rotate, thereby causing the metal wire 8 to rotate and wind to form a slender rod 22. The length of the slender rod 22 is preferably selected from 10 cm to 30 cm, and the number of rotation and winding of the slender rod 22 is preferably selected from 5 to 10 turns per 1 cm of length. The rotator 5 is stopped after the metal wire 8 is rotated and wound to a suitable number of turns. The wound metal wire 8 is separated from the hook 3, and the contact portion of the metal wire 8 and the hook 3 forms a tail loop 21; the clamp 4 is released, and the portion of the metal wire 8 fixed by the clamp 4 forms a clamping and fixing portion 7; the head and tail ends 83 of the metal wire now extend and protrude outside the clamping and fixing portion 7; The length of the first and last ends 83 of the metal wire is cut by the clamp 4 , and force is applied to change its shape so that the first and last ends 83 of the metal wire are tightly attached to the clamping and fixing part 7 ; the shape of the tail ring 21 is modified by the clamp 4 to form the tail end 23 .
[0014] After the integrated inoculation loop is manufactured, it is placed in the sterilization device 6, sterilized and dried for later use, and the sterilization method is high-pressure steam sterilization.
[0015] Dissolve all ingredients in the Lactobacillus agar medium, excluding agar, in distilled water, adjust the pH to 6.8 (25°C), add the agar, and heat until completely dissolved. Autoclave at 121°C for 15 minutes. Cool to approximately 50°C, pour into a Petri dish, and allow to cool and solidify to form a Lactobacillus agar plate. The ingredients of the Lactobacillus agar medium are as follows: Element content Peptized milk 15.0 g yeast extract 5.0 g glucose 10.0 g tomato juice 100 ml Potassium dihydrogen phosphate 2.0 g Polysorbate monooleate 1.0 g agar 10.0 g distilled water 1000 ml Separate the sterilized lid 61 from the upper portion 63 of the sterilizing device 6, rotate the upper portion 63 and the lower portion 66, and retract the sterilizing device 6 so that the handle 2 of the inoculating loop extends from the sterilizing device 6. Hold the handle 2 of the inoculating loop and remove it from the sterilizing device 6. Then, use the circle 11 to pick up a strain of Lactobacillus plantarum, streak it onto a Lactobacillus agar plate, and incubate it at 36°C for 18 hours.
[0016] The step 67 and the partition 68 structure arranged inside the sterilization device 6 separate the inoculation rings installed in the sterilization device 6 into several areas. The inoculation rings in each area have different heights. This design not only enables the integrated inoculation rings to be used in batches after sterilization, thereby improving work efficiency, but also further reduces the possibility of microorganisms on the operator's fingers being transferred to unused sterile inoculation rings through touch, thereby effectively reducing cross contamination and ensuring the accuracy and reliability of subsequent experimental results.
[0017] When the inoculating loop is used, the elastic head 1 has not only a certain elasticity but also a certain hardness, which makes it feel good. When marking, the elastic head 1 undergoes a certain deformation due to its elasticity and hardness as the hand-held force changes, always keeping the circle 11 in close contact with the culture medium on the Lactobacillus agar plate.
[0018] After the lactobacillus agar culture medium plate culture was completed, it was observed that the Lactobacillus plantarum colonies on the plate grew well. Example
[0019] A batch sterilization device with an integrated inoculation loop, when applied to the inoculation of Lactobacillus leishmanii (strain number ATCC7830), adopts the following technical solutions to achieve the beneficial effects of the present invention: A batch sterilization device containing an integrated inoculating loop, characterized in that: The batch sterilization device 6 is cylindrical in shape as a whole, and includes a cover 61, an upper portion 63, a lower portion 66 and an integrated inoculation loop; The cover 61 is cylindrical, with one end open and the other end closed. The tail end of the open portion contracts toward the center of the cylinder to form a slot 62. The slot 62 is used for connection or fixation with other parts. The upper portion 63 is a cylindrical tube-shaped structure. One end of the upper portion 63 is open and provided with an upper end thread 64. The other end of the upper portion 63 is open, and the outer diameter of the circular ring of the open section is equal to or slightly smaller than the inner diameter of the circular ring of the section of the slot 62, so as to ensure that the opening and the slot 62 can be tightly fastened. The cover 61 is fastened to the upper portion 63 through the slot 62, and the cover 61 and the upper portion 63 can be easily disassembled and tightly assembled. The lower portion 66 is a cylindrical, canister-shaped structure. One end of the lower portion 66 is open and provided with a lower thread 65. The other end of the lower portion 66 is closed and provided with at least one step 67 at the closed position. The step 67 is connected to a partition 68. The partition 68 divides the internal space of the lower portion 66 into at least two chambers 69. The outer diameter of the cut surface of the lower thread 65 is equal to or slightly smaller than the inner diameter of the cut surface of the upper thread 64, so as to ensure that the upper thread 64 and the lower thread 65 can be tightly screwed together. The upper end thread 64 of the upper part 63 is rotationally connected to the lower end thread 65 of the lower part 66. Through the rotation of the thread, the relative rotation of the upper part 63 and the lower part 66 is converted into displacement in the vertical direction of the cylinder, so as to realize the adjustment of the height of the batch sterilization device and obtain the maximum height and minimum height of the batch sterilization device.
[0020] The integrated inoculation loop includes an elastic head 1, a handheld rod 2, and a clamping and fixing portion 7; the elastic head 1 includes a circle 11 and a winding rod 12, and the handheld rod 2 includes a slender rod 22 and a tail end 23; the height of the integrated inoculation loop is less than the maximum height of the batch sterilization device, and the height of the integrated inoculation loop is greater than the minimum height of the batch sterilization device; The integrated inoculation ring is made of a metal wire 8 by bending, winding, and other steps; the diameter of the metal wire 8 is preferably selected from 0.3 mm to 1 mm, and the material of the metal wire 8 is preferably selected from stainless steel or other possible materials; During sterilization, the integrated inoculating loop is placed inside the batch sterilization device with the circle 11 facing downward and the tail end 23 facing upward. When the batch sterilization device needs to accommodate multiple integrated inoculating loops, the integrated inoculating loops are placed in multiple chambers 69 respectively. The method for making the integrated inoculating loop requires the use of auxiliary equipment such as a clamp 4, a hook 3, and a rotator 5. The hook 3 is made by bending a metal rod, and the diameter of the hook 3 is preferably selected from 1 mm to 5 mm. The production method comprises the following steps: The metal wire 8 is bent after being overlapped end to end, and a single-layer bending loop 81 is formed at the bending portion of the metal wire 8. The metal wire 8 is bent a second time, so that the end 83 of the metal wire is inserted into the single-layer bending loop 81 and fixed with the clamp 4. A double-layer bending loop 82 is formed at the bending portion. Fix the straight handle portion of the hook 3 with a rotator 5; Hang the hook 3 in the single-layer bending ring 81, and apply forces in opposite directions to the clamper 4 and the rotator 5 to straighten the metal wire 8 as much as possible; The rotator 5 is started to drive the hook 3 to rotate, so that the metal wire 8 is rotated and wound to form a winding rod 12. The length of the winding rod 12 is preferably selected from 5 cm to 10 cm, and the number of rotation and winding of the winding rod 12 is preferably selected from 5 to 15 turns per 1 cm of length. The rotator 5 is stopped after the metal wire 8 is rotated and wound to a suitable number of turns. The wound metal wire 8 is separated from the hook 3. The contact portion of the metal wire 8 and the hook 3 forms a circle 11. The inner diameter of the circle 11 is equal to the diameter of the hook 3. Therefore, the inner diameter of the circle 11 is preferably selected from 1 mm to 5 mm. Hang the hook 3 in the double-layer bending ring 82, and apply forces in opposite directions to the clamper 4 and the rotator 5 to straighten the wire 8 as much as possible; The rotator 5 is started to drive the hook 3 to rotate, thereby causing the metal wire 8 to rotate and wind to form a slender rod 22. The length of the slender rod 22 is preferably selected from 10 cm to 30 cm, and the number of rotation and winding of the slender rod 22 is preferably selected from 5 to 10 turns per 1 cm of length. The rotator 5 is stopped after the metal wire 8 is rotated and wound to a suitable number of turns. The wound metal wire 8 is separated from the hook 3, and the contact portion of the metal wire 8 and the hook 3 forms a tail loop 21; the clamp 4 is released, and the portion of the metal wire 8 fixed by the clamp 4 forms a clamping and fixing portion 7; the head and tail ends 83 of the metal wire now extend and protrude outside the clamping and fixing portion 7; The length of the first and last ends 83 of the metal wire is cut by the clamp 4 , and force is applied to change its shape so that the first and last ends 83 of the metal wire are tightly attached to the clamping and fixing part 7 ; the shape of the tail ring 21 is modified by the clamp 4 to form the tail end 23 .
[0021] After the integrated inoculation loop is manufactured, it is placed in the sterilization device 6 and sterilized for use by dry heat sterilization.
[0022] Dissolve all ingredients in the Lactobacillus agar medium, excluding agar, in distilled water, adjust the pH to 6.9 (23°C), add the agar, and heat until completely dissolved. Autoclave at 121°C for 15 minutes. Cool to approximately 50°C, then pour into a Petri dish. Allow to cool and solidify to form a Lactobacillus agar plate. Set aside. The ingredients of the Lactobacillus agar medium are as follows: Element content Peptone No. 3 7.5 g yeast extract 7.5 g glucose 10.0 g tomato juice 100 ml Potassium dihydrogen phosphate 2.0 g Polysorbate monooleate 1.0 g agar 14.0 g distilled water 1000 ml Separate the sterilized lid 61 from the upper portion 63 of the sterilizing device 6. Rotate the upper portion 63 and lower portion 66 to retract the sterilizing device 6 so that the handle 2 of the inoculating loop extends from the sterilizing device 6. Hold the handle 2 of the inoculating loop and remove it from the sterilizing device 6. Use the circle 11 to pick up a strain of Lactobacillus leishmanii and streak it onto a Lactobacillus agar plate. Incubate the plate at 36°C for 24 hours.
[0023] The step 67 and the partition 68 structure arranged inside the sterilization device 6 separate the inoculation rings installed in the sterilization device 6 into several areas. The inoculation rings in each area have different heights. This design not only enables the integrated inoculation rings to be used in batches after sterilization, thereby improving work efficiency, but also further reduces the possibility of microorganisms on the operator's fingers being transferred to unused sterile inoculation rings through touch, thereby effectively reducing cross contamination and ensuring the accuracy and reliability of subsequent experimental results.
[0024] When the inoculating loop is used, the elastic head 1 has not only a certain elasticity but also a certain hardness, which makes it feel good. When marking, the elastic head 1 undergoes a certain deformation due to its elasticity and hardness as the hand-held force changes, always keeping the circle 11 in close contact with the culture medium on the Lactobacillus agar plate.
[0025] After the lactobacillus agar culture medium plate culture was completed, it was observed that the Lactobacillus leishmanii colonies on the plate grew well.
Claims
1. A batch sterilization device containing an integrated inoculating loop, characterized in that: The batch sterilization device is cylindrical in shape as a whole and includes a cover, an upper part, a lower part and an integrated inoculation loop; The cover is cylindrical, with one end open and the other end closed, and the tail end of the opening shrinks toward the center of the cylinder to form a slot; the slot is used for connection or fixation with other parts; The upper part is a cylindrical barrel structure, one end of the upper part is open and provided with an upper end thread, the other end of the upper part is open, and the outer diameter of the ring of the opening section is equal to or slightly smaller than the inner diameter of the ring of the groove section, so as to ensure that the opening and the groove can be tightly fastened, the cover is fastened to the upper part through the groove, and the cover and the upper part can be easily disassembled and tightly assembled; The lower part is a cylindrical barrel structure, one end of the lower part is open and provided with a lower end thread, the other end of the lower part is closed, and at least one step is provided at the closed position, the step is connected to the partition, and the partition divides the internal space of the lower part into at least two chambers, the outer diameter of the circular ring of the lower end thread opening is equal to or slightly smaller than the inner diameter of the circular ring of the upper end thread section, so as to ensure that the upper end thread and the lower end thread can be tightly screwed together; The upper end thread of the upper part is rotationally connected to the lower end thread of the lower part. Through the rotation of the thread, the relative rotation of the upper part and the lower part is converted into displacement in the vertical direction of the cylinder to achieve the adjustment of the height of the batch sterilization device and obtain the maximum height and minimum height of the batch sterilization device.
2. The batch sterilization device with an integrated inoculating loop according to claim 1, characterized in that: The integrated inoculation loop includes an elastic head, a handheld rod, and a clamping and fixing portion; the elastic head includes a circle and a winding rod, and the handheld rod includes a slender rod and a tail end; the height of the integrated inoculation loop is less than the maximum height of the batch sterilization device, and the height of the integrated inoculation loop is greater than the minimum height of the batch sterilization device; The integrated inoculation ring is made of a metal wire through bending, winding and other steps; the diameter of the metal wire is preferably selected from 0.3mm to 1mm, and the material of the metal wire is preferably selected from stainless steel or other possible materials; During sterilization, the integrated inoculating loop is placed inside the batch sterilization device with the circle facing downward and the tail facing upward. When the batch sterilization device needs to accommodate multiple integrated inoculating loops, the integrated inoculating loops are placed in the multiple chambers respectively. The method for making the integrated inoculation ring requires the use of auxiliary equipment such as a clamp, a hook, and a rotator. The hook is made by bending a metal rod, and the diameter of the hook is preferably selected from 1 mm to 5 mm.
3. The batch sterilization device with an integrated inoculating loop according to claim 2, characterized in that: The method for making the integrated inoculating loop comprises the following steps: a. The metal wire is bent end to end, and then the metal wire forms a single-layer bending circle at the bend; the metal wire is bent a second time, so that the end ends of the metal wire are inserted into the single-layer bending circle and fixed with a clamp; at this time, a double-layer bending circle is formed at the bend; b. Use a rotator to fix the straight handle of the hook; c. The hook is hung in the single-layer bending circle, and forces are applied in opposite directions to the clamp and the rotator to straighten the wire as much as possible; d. Start the rotator to drive the hook to rotate, so that the wire is rotated and wound to form a winding rod. The length of the winding rod is preferably 5cm to 10cm, and the number of rotations of the winding rod is preferably 5 to 15 turns per 1cm length; the rotator is stopped after the wire is rotated and wound to the appropriate number of turns; e. The wound wire is separated from the hook, and the contact portion of the wire and the hook forms a circle, the inner diameter of the circle being equal to the diameter of the hook, so the inner diameter of the circle is preferably selected from 1mm to 5mm; f. The hook is hung in the double-layer bending circle, and forces are applied in opposite directions to the holder and the rotator to straighten the wire as much as possible; g. Start the rotator to drive the hook to rotate, thereby rotating and winding the wire to form a slender rod. The length of the slender rod is preferably 10 cm to 30 cm, and the number of rotation and winding of the slender rod is preferably 5 to 10 turns per 1 cm length; stop the rotator after the wire is rotated and wound to the appropriate number of turns; h. Separate the wound wire from the hook, forming a tail loop at the contact portion of the wire and the hook; release the clamp, and the portion of the wire held by the clamp forms a clamped portion; at this point, the first and tail ends of the wire extend protruding from the outside of the clamped portion; i. Use a clamp to cut the length of the wire ends and apply force to change its shape so that the wire ends are close to the clamping and fixing part; use a clamp to modify the shape of the tail ring to form the tail end.