Microbial fermentation culture tank for planting culture medium

Abstract

The invention relates to the technical field of microorganism culture, in particular to a planting culture medium microorganism fermentation culture tank which comprises a tank body, a motor fixedly installed at the top end of a tank cover on the upper portion of the tank body, a stirring shaft fixedly connected to the bottom end of a motor driving shaft and a stirring paddle fixedly installed on the outer side of the middle of the stirring shaft. The device is characterized in that a defoaming assembly is arranged between the outer side of the top of the stirring shaft and the inner wall of the tank body, a scraping ring assembly is arranged at the top of the defoaming assembly, and a homogenizing assembly is arranged between the bottom end of the defoaming assembly and the bottom of the stirring shaft. The rotating stirring shaft drives the defoaming paddle and the collision block to rotate, so that the collision plate collides with the collision block, then the scraping ring is driven to rotate and slide to scrape fermentation liquor on the inner wall, and meanwhile, the stirring teeth at the bottom can perform horizontal rotary stirring and linear reciprocating stirring perpendicular to the stirring shaft; and the mixing density of bottom fermentation liquor is more uniform.

Description

Technical Field

[0001] The invention relates to the technical field of microorganism cultivation, in particular to a microorganism fermentation culture tank for planting culture medium. Background Art

[0002] Microbial fermentation culture tanks (also known as bioreactors) are core equipment used for large-scale cultivation of microorganisms, cells or enzymes. Their basic working principles involve key links such as sterile environment control, nutrient supply, environmental parameter regulation and metabolite management.

[0003] The culture cycle of microorganisms in the fermentation tank is roughly divided into the lag phase, logarithmic growth phase and stable phase. During the logarithmic growth phase, the metabolic activity of microorganisms is extremely vigorous, and they will consume a large amount of carbon sources (such as sugars) and nitrogen sources in the culture medium, and produce a large amount of metabolites (such as proteins, polysaccharides, These metabolites (especially proteins) have surface activity, which will reduce the surface tension of the culture medium and promote foam formation. At the same time, the respiration of microorganisms will release , the gas forms bubbles in the culture medium and further forms foam.

[0004] Excessive foaming can cause the fermentation liquid to overflow from the top of the tank, resulting in product loss. Furthermore, the foam layer covering the liquid surface hinders oxygen transfer and reduces dissolved oxygen efficiency. Therefore, a defoaming component is required inside the fermentation tank to quickly eliminate the foam. Existing defoaming components are mostly divided into two types: one uses a rotating defoaming paddle, and the other uses ultrasonic defoaming. Both methods have certain drawbacks.

[0005] When foam is generated, due to the expansion of the foam volume, part of the fermentation liquid will adhere to the inner wall of the tank. When defoaming, the foam bursts and part of the fermentation liquid will splash onto the tank wall, resulting in the fermentation liquid hanging on the wall. The existing defoaming paddles and ultrasonic defoamers cannot touch the fermentation liquid adhering to the tank wall, and the height of the main fermentation liquid at the bottom of the tank is limited, and the fermentation liquid hanging on the wall cannot be drawn into the mixing, which will cause certain production waste.

[0006] Existing fermentation tanks are unable to further improve the mixing and stirring efficiency of the dense substances at the bottom of the fermentation liquid during defoaming. During the logarithmic growth period when the bacteria produce foam, the bacteria will produce a large amount of metabolites, which will make the fermentation liquid as a whole become dense. When the metabolites settle to the bottom of the tank, they will affect the overall density of the fermentation liquid. The uneven fermentation liquid will reduce the bacteria's efficiency in absorbing nutrients. Summary of the Invention

[0007] The object of the present invention is to provide a microbial fermentation culture tank for a cultivation medium which can physically defoam by collision, scrape off nutrients on the tank wall and make the density distribution of the fermentation liquid more uniform.

[0008] To achieve the above objectives, the present invention is implemented through the following technical solutions: a planting culture medium microbial fermentation culture tank, comprising a tank body, a motor fixedly installed on the top of the tank cover on the upper part of the tank body, a stirring shaft fixedly connected to the bottom end of the motor drive shaft and a stirring paddle fixedly installed on the outside of the middle part of the stirring shaft, a defoaming assembly is provided between the outside of the top of the stirring shaft and the inner wall of the tank body, a scraper ring assembly is provided on the top of the defoaming assembly, and a homogenizing assembly is provided between the bottom end of the defoaming assembly and the bottom of the stirring shaft.

[0009] The defoaming assembly includes a defoaming paddle fixedly mounted on the outside of the top of the stirring shaft, a collision block fixedly connected to one end of the defoaming paddle, a rebound structure arranged on the inner wall of the tank body, a sliding rod arranged on the inner side of the rebound structure and a collision plate fixedly connected to the top of the sliding rod, and a convex ball is fixedly connected to the top of the collision plate.

[0010] The scraper ring assembly comprises a fixed ring fixedly connected to the outer side of the top of the slide rod and a scraper ring rotatably connected to the inner side of the outer ring of the fixed ring.

[0011] The homogenizing assembly includes a retaining ring fixedly connected to the bottom end of the slide rod, a telescopic tube structure arranged on the outside of the bottom of the stirring shaft, and T-shaped stirring teeth arranged on the outside of the telescopic tube structure. The inner ring of the retaining ring is narrow at the top and wide at the bottom, and one end of the telescopic tube structure touches the inner ring of the retaining ring.

[0012] Furthermore, the bottom ends of the defoaming paddle and the collision block are fixedly connected with spike-shaped crushing teeth, which extend from the end where the defoaming paddle is connected to the stirring shaft to the collision block. The teeth are arranged from large to small, and the tooth spacing is arranged from sparse to dense.

[0013] Furthermore, the collision plate is slidably connected to the collision block, and a tooth groove is provided on the top of the collision plate, which engages with the crushing teeth fixedly connected to the bottom end of the collision block. The collision block and the crushing teeth at the bottom form a lower semicircular bulge as a whole. When the collision block collides with the convex ball, the collision block presses the convex ball down.

[0014] Furthermore, the rebound structure includes a support tube fixedly connected to the inner wall of the tank, an extrusion ring slidably connected to the inner side of the support tube, and a spring 1 fixedly connected to the bottom end of the extrusion ring and the inner bottom end of the support tube, and the extrusion ring is fixedly connected to the outer side of the sliding rod.

[0015] Furthermore, shaft seals are provided at the sliding connections between the pipe ends at both ends of the support tube and the sliding rod, and the sliding rod slides inside the support tube, and the inside of the support tube remains sealed.

[0016] Furthermore, a spiral limiting strip is fixedly connected to the inner wall of the tank body, and a limiting groove is provided on the outer side of the scraper ring and is slidably connected to the limiting strip.

[0017] Furthermore, the telescopic tube structure includes a connecting rod fixedly installed on the outside of the bottom of the stirring shaft and a telescopic tube slidably connected to the outside of the connecting rod. The outside of the telescopic tube is fixedly connected to the stirring teeth, and the end of the connecting rod away from the stirring shaft is fixedly connected to an extrusion block. A spring 2 is fixedly connected between the extrusion block and the end of the inner side of the telescopic tube away from the stirring shaft.

[0018] Furthermore, a groove is provided on the inner wall of the telescopic tube, a convex block is fixedly connected to the outer side of the extrusion block, and the convex block is slidably connected to the groove.

[0019] Furthermore, a ball is sleeved on the inner side of one end of the telescopic tube close to the retaining ring, and the ball is rollingly connected to the inner ring of the retaining ring.

[0020] Furthermore, a shaft seal is provided at the sliding connection between the tube opening of the telescopic tube and the connecting rod. The telescopic tube slides on the outside of the connecting rod, and the inside of the telescopic tube remains sealed.

[0021] The present invention provides a microbial fermentation culture tank for a planting culture medium, which has the following beneficial effects:

[0022] (1) The present invention is provided with a dual-effect defoaming component based on the original defoaming paddle. Not only can the defoaming be performed by rotating the defoaming paddle, but the foam that is swung to the inner edge of the tank body by the defoaming paddle can also be quickly impacted and crushed, thereby improving the crushing efficiency. At the same time, a scraping ring component is provided on the outside of the defoaming component. When the defoaming is performed by impacting up and down, the fermentation liquid hanging on the inner wall of the tank body can be scraped back into the main fermentation liquid, thereby reducing production waste. Moreover, the scraping ring in the scraping ring component can spin when scraping up and down, and can throw away the nutrients adhering to the scraping ring, thereby having a certain self-cleaning function.

[0023] (2) The present invention is provided with a homogenizing component at the bottom end of the defoaming component. The T-shaped stirring teeth of the homogenizing component can not only perform horizontal rotation stirring under the drive of the stirring shaft, but also perform reciprocating linear stirring along the axial direction of the vertical stirring shaft under the drive of the defoaming component, so that the overall density of the fermentation liquid is uniform, thereby improving the mixing efficiency of the fermentation liquid with high density. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the embodiments or the description of the prior art. Obviously, the drawings described below are merely exemplary, and those skilled in the art can, without inventive effort, derive other implementation drawings based on the provided drawings.

[0025] Figure 1 The present invention is a three-dimensional microbial fermentation culture tank for growing culture medium. Figure 1 .

[0026] Figure 2 The present invention is a three-dimensional microbial fermentation culture tank for growing culture medium. Figure 2 .

[0027] Figure 3 It is a cross-sectional view of a microbial fermentation culture tank for a planting culture medium according to the present invention.

[0028] Figure 4 It is a three-dimensional diagram of the parts of the collision plate and the defoaming paddle of the present invention.

[0029] Figure 5 This invention Figure 3 Magnified view of area A in center.

[0030] Figure 6 This invention Figure 3 Magnified view of area B.

[0031] Figure 7 This invention Figure 3 Magnified view of area C in the middle.

[0032] Indications in the figure: 1. Tank body; 11. Cooling interlayer; 12. Water inlet pipe; 13. Drain pipe; 14. Air inlet pipe; 15. Steam pipe; 16. Discharge pipe; 2. Tank cover; 21. Exhaust pipe; 22. Culture medium filling pipe; 23. Bacteria filling pipe; 24. Observation window; 25. Temperature probe; 26. Dissolved oxygen probe; 27. pH probe; 3. Motor; 4. Stirring shaft; 5. Stirring paddle; 6. Defoaming assembly; 61. Defoaming paddle; 62. Collision block ; 63. Slide rod; 64. Collision plate; 65. Convex ball; 7. Rebound structure; 71. Support tube; 72. Extrusion ring; 73. Spring one; 8. Scraper ring assembly; 81. Fixed ring; 82. Scraper ring; 83. Limiting strip; 84. Limiting groove; 9. Homogenizing assembly; 91. Retaining ring; 92. Stirring tooth; 10. Telescopic tube structure; 101. Connecting rod; 102. Telescopic tube; 103. Groove; 104. Extrusion block; 105. Spring two; 106. Ball. DETAILED DESCRIPTION

[0033] To make the above-mentioned objects, features, and advantages of the present invention more readily apparent, specific embodiments of the present invention are described in detail below with reference to the accompanying drawings. The following description sets forth numerous specific details to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways than those described below, and those skilled in the art may make similar modifications without departing from the scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0034] See also Figure 1 、 Figure 2 and Figure 3As shown, a microbial fermentation culture tank for a cultivation medium comprises a tank body 1, a tank cover 2 bolted to the top of the tank body 1, a motor 3 bolted to the top of the tank cover 2, a stirring shaft 4 fixedly connected to the bottom end of the drive shaft of the motor 3, and a stirring paddle 5 fixed to the outside of the middle of the stirring shaft 4 via bolts and a collar. A sealing ring is provided at the connection between the tank body 1 and the tank cover 2 to maintain a sealed interior of the tank body 1, and a shaft seal is provided at the rotary connection between the stirring shaft 4 and the tank cover 2 to maintain a rotary seal.

[0035] It should be noted that an exhaust pipe 21 is fixedly connected to one side of the top of the tank cover 2, a culture medium filling pipe 22 is provided on one side of the exhaust pipe 21, a culture medium filling pipe 23 is provided on one side of the culture medium filling pipe 22, a temperature detector 25, a dissolved oxygen detector 26 and a pH detector 27 are installed on one side of the top of the tank cover 2, and an observation window 24 is provided on the top of the other side of the tank cover 2 relative to the culture medium filling pipe 23.

[0036] The inside of the tank body 1 is a fermentation bin, and the outside of the tank wall of the tank body 1 is wrapped with a cooling interlayer 11. A water inlet pipe 12 is provided at the bottom of one side of the cooling interlayer 11, and a drainage pipe 13 is provided at the top of the other side of the cooling interlayer 11. An air inlet pipe 14 and a steam pipe 15 are respectively provided on both sides of the bottom end of the tank body 1, and a discharge pipe 16 is fixedly connected to the center of the bottom end of the tank body 1.

[0037] As an embodiment of the present invention, before fermentation, the interior of the tank body 1 needs to be disinfected, and 121°C steam is filled into the tank body 1 through the steam pipe 15 for high-temperature disinfection for minutes, and then discharged through the exhaust pipe 21. After disinfection, it is quickly cooled, and cooling water is added to the cooling interlayer 11 through the water inlet pipe 12. After absorbing heat, it is discharged through the drain pipe 13. The cooling interlayer 11 can also maintain the interior of the tank body 1 at a certain temperature.

[0038] The culture medium and bacterial strain are added into the tank body 1 through the culture medium filling tube 22 and the bacterial strain filling tube 23 respectively, and the culture is started through the detection of the temperature detector 25, the dissolved oxygen detector 26 and the pH detector 27.

[0039] During the fermentation process, the motor 3 can be started to drive the stirring shaft 4 to rotate, so that the stirring paddle 5 stirs the fermentation liquid, and the bacterial strain and the culture medium can be fully mixed.

[0040] See also Figure 3 、 Figure 4 、 Figure 5 and Figure 6As shown, a defoaming assembly 6 is provided between the outer side of the top of the stirring shaft 4 and the inner wall of the tank body 1. The defoaming assembly 6 includes a defoaming paddle 61 fixedly installed on the outer side of the top of the stirring shaft 4 by a ring and bolts, a collision block 62 fixedly connected to one end of the defoaming paddle 61, a rebound structure 7 provided on the inner wall of the tank body 1, a slide rod 63 provided on the inner side of the rebound structure 7 and a collision plate 64 fixedly connected to the top of the slide rod 63, a convex ball 65 is fixedly connected to the top of the collision plate 64, and the bottom end of the defoaming paddle 61 and the bottom end of the collision block 62 are fixedly connected with spike-shaped crushing teeth, which extend from the end where the defoaming paddle 61 is connected to the stirring shaft 4 to the collision block 62, and the teeth are from large to small, and the tooth spacing is from sparse to dense. The collision plate 64 is slidably connected to the collision block 62. A tooth groove is provided at the top of the collision plate 64, which engages with the crushing teeth fixedly connected to the bottom end of the collision block 62. The collision block 62 and the crushing teeth at the bottom form a lower semicircular protrusion as a whole. When the collision block 62 collides with the convex ball 65, the collision block 62 presses the convex ball 65 downward.

[0041] The rebound structure 7 includes a support tube 71 fixedly connected to the inner wall of the tank body 1, an extrusion ring 72 slidably connected to the inner side of the support tube 71, and a spring 73 fixedly connected to the bottom end of the extrusion ring 72 and the inner bottom end of the support tube 71. The extrusion ring 72 is fixedly connected to the outer side of the slide rod 63. A shaft seal is provided at the sliding connection between the pipe ends of the support tube 71 and the slide rod 63. The slide rod 63 slides on the inside of the support tube 71, and the inside of the support tube 71 remains sealed.

[0042] As an embodiment of the present invention, when the stirring shaft 4 rotates, the stirring shaft 4 can drive the defoaming paddle 61 fixed to its outside by a ring and bolts to rotate. The teeth of the crushing teeth at the bottom end of the defoaming paddle 61 are from large to small, and the tooth spacing is from sparse to dense. During rotation, the foam can be pushed to between the collision plate 64 and the collision block 62 by centrifugal force. At the same time, the defoaming paddle 61 can also crush part of the generated foam through the crushing teeth opened at the bottom end.

[0043] When the collision block 62 hits the downward-pressing convex ball 65, the collision plate 64 drives the slide rod 63 to move downward, so that the extrusion ring 72 fixedly welded to the outside of the slide rod 63 presses down the spring 1 73. When the collision block 62 moves away from the convex ball 65, the spring 1 73 rebounds quickly, pushing the extrusion ring 72 to rise, so that the slide rod 63 pushes the extrusion plate 64 to rise, and the extrusion plate 64 and the collision block 62 collide with each other, squeezing and crushing the foam sandwiched between the two.

[0044] See also Figure 3 、 Figure 4 、 Figure 5 and Figure 6As shown, a scraper ring assembly 8 is provided on the top of the defoaming assembly 6. The scraper ring assembly 8 includes a fixed ring 81 fixedly connected to the outer side of the top of the slide rod 63 and a scraper ring 82 rotatably connected to the inner side of the outer ring of the fixed ring 81. A spiral limiting strip 83 is fixedly connected to the inner wall of the tank body 1, and a limiting groove 84 is provided on the outer side of the scraper ring 82 for sliding connection with the limiting strip 83.

[0045] As an embodiment of the present invention, when the slide rod 63 moves up and down, it drives the fixed ring 81 to move up and down, and the fixed ring 81 drives the scraper ring 82 to move up and down, scraping the fermentation liquid with broken foam adhering to the inner wall of the tank body 1 into the main fermentation liquid. In the process of the scraper ring 82 moving up and down, the spiral limiting bar 83 limits the up and down movement of the scraper ring 82 through the limiting groove 84, so that the scraper ring 82 rotates 30° under the restriction of the limiting bar 83. As the scraper ring 82 moves up and down, the scraper ring 82 itself also rotates back and forth, throwing the fermentation liquid adhering to the scraper ring 82 into the main fermentation liquid.

[0046] See also Figure 3 and Figure 7 As shown, a homogenizing component 9 is provided between the bottom end of the defoaming component 6 and the bottom of the stirring shaft 4. The homogenizing component 9 includes a retaining ring 91 fixedly connected to the bottom end of the slide rod 63, a telescopic tube structure 10 provided on the outside of the bottom of the stirring shaft 4, and a T-shaped stirring tooth 92 provided on the outside of the telescopic tube structure 10. The inner ring of the retaining ring 91 is narrow at the top and wide at the bottom, and one end of the telescopic tube structure 10 touches the inner ring of the retaining ring 91.

[0047] The telescopic tube structure 10 includes a connecting rod 101 fixedly installed on the outside of the bottom of the stirring shaft 4 by a ring and bolts, and a telescopic tube 102 slidably connected to the outside of the connecting rod 101. The outside of the telescopic tube 102 is fixedly connected to the stirring teeth 92. The end of the connecting rod 101 away from the stirring shaft 4 is fixedly connected to an extrusion block 104, and a spring 2 105 is fixedly connected between the extrusion block 104 and the end of the inner side of the telescopic tube 102 away from the stirring shaft 4.

[0048] A groove 103 is provided on the inner wall of the telescopic tube 102, and a protrusion is fixedly connected to the outside of the extrusion block 104. The protrusion is slidably connected to the groove 103. A ball 106 is sleeved on the inner side of one end of the telescopic tube 102 close to the retaining ring 91. The ball 106 is rollingly connected to the inner ring of the retaining ring 91. A shaft seal is provided at the sliding connection between the tube end of the telescopic tube 102 and the connecting rod 101. The telescopic tube 102 slides on the outside of the connecting rod 101, and the inside of the telescopic tube 102 remains sealed.

[0049] As an embodiment of the present invention, when the stirring shaft 4 rotates, the stirring shaft 4 can drive the connecting rod 101 fixedly installed on and outside thereof by a ring and bolts to rotate horizontally, driving the T-shaped stirring teeth 92 on the outside of the telescopic tube 102 to rotate and stir the sediment at the bottom of the fermentation liquid.

[0050] When the slide bar 63 moves up and down, the baffle ring 91 at the bottom end is driven to move up and down. Since the inner side of the cross-section of the baffle ring 91 is narrow at the top and wide at the bottom, when the baffle ring 91 moves down, the distance between the baffle ring 91 and the stirring shaft 4 increases, and the second spring 105 pushes the telescopic tube 102 close to the baffle ring 91, so that the T-shaped stirring teeth 92 can move in the direction perpendicular to the stirring shaft 4. When the baffle ring 91 moves up, the distance between the baffle ring 91 and the stirring shaft 4 decreases, and the baffle ring 91 pushes the telescopic tube 102 to contract through the ball 106, compressing the second spring 105, so that the T-shaped stirring teeth 92 can move in the opposite direction perpendicular to the stirring shaft 4, and so on. The T-shaped stirring teeth 92 produce multi-directional stirring, reducing the density of the bottom of the fermentation liquid, and making the overall density of the fermentation liquid more uniform.

[0051] In the embodiments of the present invention, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Furthermore, a first feature being "above," "above," or "above" a second feature may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is at a higher level than the second feature. A first feature being "below," "below," or "below" a second feature may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature is at a lower level than the second feature.

[0052] In the description of the present invention, it should also be noted that, unless otherwise expressly specified or limited, the terms "disposed," "connected," "installed," and "connected" should be understood in a broad sense. For example, they may refer to fixed or detachable connections, integral or sliding connections; mechanical or electrical connections; direct or indirect connections through an intermediate medium; or internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0053] The embodiments of this specific implementation method are all preferred embodiments of the present invention and are not intended to limit the scope of protection of the present invention. Therefore, any equivalent changes made based on the structure, shape, and principle of the present invention should be included in the scope of protection of the present invention.

Claims

1. A microbial fermentation culture tank for a cultivation medium, comprising a tank body, a motor fixedly mounted on the top of a tank cover on the upper portion of the tank body, a stirring shaft fixedly connected to the bottom end of a drive shaft of the motor, and a stirring paddle fixedly mounted on the outer side of the middle portion of the stirring shaft, characterized in that: A defoaming assembly is provided between the outer side of the top of the stirring shaft and the inner wall of the tank, a scraper ring assembly is provided on the top of the defoaming assembly, and a homogenizing assembly is provided between the bottom end of the defoaming assembly and the bottom of the stirring shaft; The defoaming assembly includes a defoaming paddle fixedly mounted on the outside of the top of the stirring shaft, a collision block fixedly connected to one end of the defoaming paddle, a rebound structure provided on the inner wall of the tank, a sliding rod provided on the inner side of the rebound structure, and a collision plate fixedly connected to the top of the sliding rod, wherein the top of the collision plate is fixedly connected to a convex ball; The scraper ring assembly includes a fixed ring fixedly connected to the outer side of the top of the slide rod and a scraper ring rotatably connected to the inner side of the outer ring of the fixed ring; The homogenizing assembly includes a retaining ring fixedly connected to the bottom end of the slide rod, a telescopic tube structure arranged on the outside of the bottom of the stirring shaft, and T-shaped stirring teeth arranged on the outside of the telescopic tube structure. The inner ring of the retaining ring is narrow at the top and wide at the bottom, and one end of the telescopic tube structure touches the inner ring of the retaining ring.

2. The microbial fermentation culture tank for planting culture medium according to claim 1, characterized in that: The bottom of the defoaming paddle and the bottom of the collision block are fixedly connected with spike-shaped crushing teeth. The crushing teeth extend from the end where the defoaming paddle is connected to the stirring shaft to the collision block. The teeth are from large to small, and the tooth spacing is from sparse to dense.

3. The microbial fermentation culture tank for planting culture medium according to claim 2, characterized in that: The collision plate is slidably connected to the collision block. A tooth groove is provided on the top of the collision plate. The tooth groove engages with the crushing teeth fixedly connected to the bottom end of the collision block. The collision block and the crushing teeth at the bottom form a lower semicircular bulge as a whole. When the collision block collides with the convex ball, the collision block presses the convex ball down.

4. The microbial fermentation culture tank for planting culture medium according to claim 1, characterized in that: The rebound structure includes a support tube fixedly connected to the inner wall of the tank body, an extrusion ring slidably connected to the inner side of the support tube, and a spring fixedly connected to the bottom end of the extrusion ring and the inner bottom end of the support tube. The extrusion ring is fixedly connected to the outer side of the slide rod.

5. The microbial fermentation culture tank for planting culture medium according to claim 4, characterized in that: A shaft seal is provided at the sliding connection between the pipe openings at both ends of the support tube and the sliding rod. The sliding rod slides inside the support tube, and the inside of the support tube remains sealed.

6. The microbial fermentation culture tank for planting culture medium according to claim 1, characterized in that: A spiral limiting strip is fixedly connected to the inner wall of the tank body, and a limiting groove which is slidably connected to the limiting strip is provided on the outer side of the scraper ring.

7. The microbial fermentation culture tank for planting culture medium according to claim 1, characterized in that: The telescopic tube structure includes a connecting rod fixedly installed on the outside of the bottom of the stirring shaft and a telescopic tube slidably connected to the outside of the connecting rod. The outside of the telescopic tube is fixedly connected to the stirring teeth. The end of the connecting rod away from the stirring shaft is fixedly connected to an extrusion block. A spring 2 is fixedly connected between the extrusion block and the end of the inner side of the telescopic tube away from the stirring shaft.

8. The microbial fermentation culture tank for planting culture medium according to claim 7, characterized in that: A groove is provided on the inner wall of the telescopic tube, a convex block is fixedly connected to the outer side of the extrusion block, and the convex block is slidably connected to the groove.

9. The microbial fermentation culture tank for planting culture medium according to claim 7, characterized in that: A ball is sleeved on the inner side of one end of the telescopic tube close to the retaining ring, and the ball is in rolling connection with the inner ring of the retaining ring.

10. The microbial fermentation culture tank for planting culture medium according to claim 7, characterized in that: A shaft seal is provided at the sliding connection between the telescopic tube opening and the connecting rod. The telescopic tube slides on the outside of the connecting rod, and the inside of the telescopic tube remains sealed.

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