Energy-gathering type ultrasonic crushing / cleaning device based on acoustic superstructure

Abstract

The invention discloses an energy-gathering type ultrasonic crushing / cleaning device based on an acoustic superstructure. Comprising a high-power ultrasonic transducer, an acoustic superstructure groove, cooling liquid, a cylindrical groove, a power pump, a biological cell solution test tube, a cooling liquid conveying pipe and the like. When the device works, the piezoelectric plate receives electric energy and generates ultrasonic vibration, the vibration energy is transmitted to the acoustic superstructure groove through efficient propagation of the aluminum plate, the ultrasonic energy is focused in a biological test tube solution area in the groove through the groove, and a high-strength ultrasonic field is formed; meanwhile, the device can also be used for cleaning long and thin test tubes with the height-diameter ratio, narrow inner cavities and easy deposition at the bottom or adhesion to the side walls. According to the invention, the energy gathering characteristic of the acoustic superstructure is utilized, and the acoustic superstructure groove is combined with the high-power ultrasonic transducer, so that the energy density of a crushing area is remarkably improved, cells are crushed more sufficiently, cleaning procedures before and after a test are reduced, the crushing quality and the experiment efficiency are improved, and the experiment accuracy is enhanced.

Description

Technical Field

[0001] The invention belongs to the technical field of acoustic devices and ultrasonic crushing, and relates to an energy-concentrating ultrasonic crushing / cleaning device based on an acoustic superstructure. Background Art

[0002] In the fields of biological experiments, chemical analysis and materials research, cell disruption and special test tube cleaning are both essential parts of the experiment. On the one hand, although traditional ultrasonic disruption devices can use the ultrasonic cavitation effect to disrupt cells, they have problems such as energy dispersion and poor targeting, making it difficult to accurately and efficiently act on the solution in the test tube, which not only affects the efficiency of cell disruption, but also easily causes interference between samples. On the other hand, traditional ultrasonic cleaning equipment also faces cleaning difficulties when dealing with long and thin test tubes with high diameter ratios and narrow lumens, such as centrifuge tubes and micro-injection bottles: because ultrasonic energy is difficult to penetrate into the narrow lumen and cannot effectively act on the bottom and side walls of the test tube, it is difficult to completely remove the sediment at the bottom of the test tube and the adhesions on the side walls, affecting the accuracy of the experimental results and the reusability of the test tube. In addition, traditional equipment lacks adaptability design for different types of test tubes and cannot meet the diverse experimental needs, resulting in low experimental efficiency and increased costs.

[0003] Therefore, there is an urgent need for a new type of ultrasonic fragmentation and special test tube cleaning device that can effectively focus ultrasonic energy, reduce the accuracy of its effect on the target sample, and improve experimental efficiency and energy utilization efficiency, so as to address the shortcomings of traditional technology and improve experimental quality and efficiency. Summary of the Invention

[0004] Purpose of the invention: The purpose of the present invention is to provide an energy-concentrating ultrasonic crushing / cleaning device based on an acoustic superstructure that can solve the problems of poor ultrasonic crushing targeting and difficult test tube cleaning, and can achieve non-contact and efficient ultrasonic crushing / cleaning.

[0005] The technical solution of the present invention is as follows: the energy-focusing ultrasonic crushing / cleaning device based on an acoustic superstructure comprises a high-power ultrasonic transducer, an acoustic superstructure groove, a coolant, a cylindrical tank, a power pump, a biological cell solution test tube, a coolant delivery pipe, etc.; the high-power ultrasonic transducer device involves a piezoelectric piece, a metal component aluminum plate, etc.;

[0006] An acoustic superstructure groove is installed inside the cylindrical groove, and the acoustic superstructure groove is connected to the power pump through an installed coolant delivery pipe.

[0007] Furthermore, the metal components in the high-power ultrasonic transducer are made of aluminum. Metal aluminum has excellent sound propagation characteristics and can transmit ultrasonic vibrations quickly and with low loss. The metal components play a role in structural support and auxiliary energy conduction adaptation. Multiple components work together to achieve stable excitation and initial conduction of ultrasonic energy.

[0008] Furthermore, a test tube groove is installed inside the acoustic superstructure groove, and a biological cell solution test tube is installed in the test tube groove.

[0009] Furthermore, aluminum plate 2 and aluminum plate 1 are respectively installed at the bottom ends of the cylindrical groove, and piezoelectric plate 3, piezoelectric plate 2, and piezoelectric plate 1 are installed between aluminum plate 2 and aluminum plate 1.

[0010] Furthermore, the acoustic superstructure groove is made of aluminum and adapted to the specific shape of the biological cell solution test tube; the structural design is based on the acoustic superstructure principle, which can focus the energy transmitted by the ultrasonic transducer, so that the ultrasonic energy is concentrated on the biological test tube solution in the groove, forming a high-intensity, high-energy-density ultrasonic field at the focal point, making the energy distribution of the equipment more concentrated and enhancing the cell disruption effect.

[0011] Furthermore, the power pump is a peristaltic pump without valves and impellers; the liquid is transported through an extrusion hose to avoid direct contact between the liquid and the pump body, and the flow rate is stable and the pulsation is small, which does not affect the stability of the ultrasonic field.

[0012] Furthermore, the coolant in the coolant delivery pipe is ionized water, which has low cost, large specific heat capacity, high heat dissipation efficiency, and is non-conductive, thus avoiding the risk of short circuit to electronic components such as piezoelectric pieces. At the same time, the temperature is kept at 25°C to prevent cell proteins from being inactivated by high temperature, thereby hindering the accuracy of the experiment.

[0013] Furthermore, the input voltage range of the high-power ultrasonic transducer is set to 1500-8000VAC, and after conversion processing by the power conversion module, it can output a high-frequency electrical signal of 40-120kHz.

[0014] Furthermore, the piezoelectric sheet three, the piezoelectric sheet two and the piezoelectric sheet one are mounted on the aluminum plate two and the aluminum plate one by welding, and are connected to an external power drive circuit;

[0015] The piezoelectric piece three, piezoelectric piece two and piezoelectric piece one can stably generate a high-frequency electrical signal of 20-40kHz when driven by a voltage of 5-20VDC, and the amplitude fluctuation of the output signal is strictly controlled within an error range of ±5%.

[0016] Furthermore, the device designs high-power ultrasonic transducers, acoustic superstructure grooves, cooling systems, etc. as independent modular components, which can be quickly disassembled and replaced according to experimental requirements, making the equipment more scalable and adaptable.

[0017] Furthermore, the energy-focused ultrasonic fragmentation / cleaning device adopts an array-type multi-slot structure design, which can accommodate multiple biological test tubes for parallel experiments at the same time, and by optimizing the acoustic isolation design between holes, it avoids ultrasonic interference between adjacent samples, thereby realizing batch cell fragmentation processing and meeting high-throughput cleaning needs.

[0018] Furthermore, the inverted groove structure has built-in elastic clamping and optical positioning components, which can automatically identify the size of the test tube and adjust the fixing force to ensure that the sample is accurately placed in the center of the ultrasonic focus.

[0019] Beneficial Effects: Compared with existing technologies, this invention has the following significant features: 1. The device utilizes acoustic superstructure grooves to focus ultrasonic energy on the biological test tube solution area within the groove, forming a high-intensity ultrasonic field, significantly increasing the energy density in the disruption zone and ensuring more complete cell disruption. 2. The device can simultaneously accommodate multiple biological test tubes for parallel experiments, enabling batch cell disruption and high-throughput cleaning, reducing pre- and post-experimental cleaning steps and improving experimental efficiency. 3. The device utilizes an array-type multi-slot design, with each component being independently modularized and quickly disassembled and replaced according to experimental needs. This design can meet diverse experimental requirements and offers strong scalability and adaptability. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram of the device array multi-slot structure of the present invention;

[0021] Figure 2 This is a schematic diagram of the installation of the acoustic superstructure groove, biological cell solution test tube and test tube tank in the present invention;

[0022] Figure 3 This is a schematic diagram of the cooling structure of the power pump in the device of the present invention Figure 1 ;

[0023] Figure 4 This is a schematic diagram of the cooling structure of the power pump in the device of the present invention Figure 2 ;

[0024] In the figure: 1 is aluminum plate 1, 2 is piezoelectric piece 1, 3 is piezoelectric piece 2, 4 is piezoelectric piece 3, 5 is aluminum plate 2, 6 is acoustic superstructure groove, 7 is biological cell solution test tube, 8 is test tube groove, 9 is cylindrical groove, 10 is power pump, and 11 is coolant delivery pipe. DETAILED DESCRIPTION

[0025] The specific technical solutions of the present invention are further described in detail below with reference to specific examples.

[0026] As shown in the figure, the energy-focusing ultrasonic fragmentation / cleaning device based on an acoustic superstructure described in the present invention uses an external power supply to drive a piezoelectric plate to generate a high-frequency electrical signal. This signal is converted into mechanical vibration by an ultrasonic transducer and transmitted to the acoustic superstructure groove 6. Leveraging the focusing properties of the groove surface, a high-intensity focused ultrasonic field is formed in the region of a biological cell solution test tube 7. When the device is operating, the piezoelectric plate receives electrical energy and converts it into ultrasonic vibration. This vibration is efficiently transmitted to the acoustic superstructure groove 6 via an aluminum plate. Utilizing the energy-focusing properties of the acoustic superstructure, the energy is focused on the test tube solution, achieving cell fragmentation or test tube cleaning through ultrasonic cavitation and mechanical vibration.

[0027] The device includes an aluminum plate 1, a piezoelectric piece 2, a piezoelectric piece 3, a piezoelectric piece 3 4, an aluminum plate 2 5, an acoustic superstructure groove 6, a biological cell solution test tube 7, a test tube groove 8, a cylindrical groove 9, a power pump 10 and a coolant delivery pipe 11; wherein, the power pump 10 drives the ion water coolant to circulate and dissipate heat to maintain a constant temperature environment of 25°C. The array-type multi-groove structure can accommodate multiple test tubes at the same time, and the acoustic isolation design between holes is combined to avoid sample interference. The modular components support rapid disassembly and replacement, which is suitable for biological cell disruption and efficient cleaning scenarios of high-diameter ratio test tubes.

[0028] An acoustic superstructure groove / cylindrical groove is bonded to the surface of the high-power ultrasonic transducer, and a power pump 10 and an acoustic superstructure groove 6 are installed inside the acoustic superstructure groove / cylindrical groove.

[0029] The metal components in the high-power ultrasonic transducer are made of aluminum. Metal aluminum has excellent sound propagation characteristics and transmits ultrasonic vibrations quickly and with low loss. The metal components play a role in structural support and auxiliary energy conduction adaptation. Multiple components work together to achieve stable excitation and initial conduction of ultrasonic energy.

[0030] The acoustic superstructure groove 6 is made of aluminum and is adapted to the specific shape of the biological cell solution test tube 7. The structural design is based on the principle of acoustic superstructure, which can focus the energy transmitted by the ultrasonic transducer, so that the ultrasonic energy is concentrated on the biological test tube solution in the groove, forming a high-intensity, high-energy-density ultrasonic field at the focal point, making the energy distribution of the equipment more concentrated and enhancing the cell disruption effect.

[0031] The coolant is ionized water, which has low cost, large specific heat capacity, high heat dissipation efficiency, and is non-conductive, thus avoiding the risk of short circuits to electronic components such as piezoelectric pieces. At the same time, the temperature is controlled at 25°C to prevent cell proteins from being inactivated by high temperatures, which would hinder the accuracy of the experiment.

[0032] The power pump 10 is a peristaltic pump without valves and impellers. It delivers liquid through a squeeze hose to avoid direct contact between the liquid and the pump body. It also has a stable flow rate and small pulsation, and does not affect the stability of the ultrasonic field.

[0033] The energy-focused ultrasonic disruption / cleaning device adopts an array-type multi-slot structure design, which can accommodate multiple biological test tubes at the same time for parallel experiments. By optimizing the acoustic isolation design between holes, ultrasonic interference between adjacent samples is avoided, thereby achieving batch cell disruption processing and meeting high-throughput cleaning needs.

[0034] The input voltage range of the high-power ultrasonic transducer is set to 1500-8000VAC, and after conversion processing by the power conversion module, it can output a high-frequency electrical signal of 40-120kHz.

[0035] The piezoelectric chip in the high-power ultrasonic transducer is welded and mounted on a circuit board in the device and connected to an external power drive circuit. Driven by a voltage of 5-20VDC, the piezoelectric chip can stably generate a high-frequency electrical signal of 20-40kHz, and the amplitude fluctuation of the output signal is strictly controlled within an error range of ±5%.

[0036] The device designs the high-power ultrasonic transducer, the acoustic superstructure groove 6, the cooling system, etc. as independent modular components, which can be quickly disassembled and replaced according to experimental requirements, making the equipment more expandable and adaptable.

[0037] The inverted groove structure has built-in elastic clamping and optical positioning components, which can automatically identify the size of the test tube and adjust the fixing force to ensure that the sample is accurately placed in the center of the ultrasonic focus.

[0038] Example

[0039] This implementation case is an application practice of an energy-focusing ultrasonic fragmentation device based on an acoustic superstructure. The core parameters of the device are designed as follows: the input voltage of the ultrasonic transducer is in the range of 1500-8000V AC, which is converted into a 40-120kHz high-frequency electrical signal by the power module. The piezoelectric plate generates 20-40kHz vibration under 5-20V DC drive with an amplitude fluctuation of ≤±5%; the acoustic superstructure groove 6 is made of aluminum, and the groove depth and other parameters are designed according to the principle of acoustic focusing, so that the energy density of the focusing area is increased by more than 40%; the cooling system uses deionized water as the coolant, which is driven by a peristaltic pump to circulate and maintain a constant temperature of 25°C; the array structure can simultaneously accommodate 12 biological cell solution test tubes 7, and the acoustic isolation spacing between the holes is ≥15mm to avoid interference.

[0040] The implementation process is divided into three phases:

[0041] During the preparation stage, the biological cell solution is placed in a test tube and then placed in the test tube slot 8. The elastic clamping of the inverted slot structure and the automatic calibration of the optical positioning component ensure that the test tube is in the focusing center of the groove, with a positioning error of ≤0.1mm. At the same time, ionized water coolant is injected into the cylindrical slot 9, and the power pump 10 is started to adjust the flow rate to 500mL / min to maintain 25°C.

[0042] During the crushing process, after the power is turned on, the piezoelectric piece receives the voltage and generates a high-frequency electrical signal, which is transmitted to the groove through the aluminum plate. The groove uses the energy-gathering characteristics to focus the energy on the test tube solution area, forming an energy density of ≥2W / mm 2 The cells were disrupted by cavitation effect and mechanical vibration for 15 minutes using a high-intensity ultrasound field.

[0043] During dynamic monitoring, if the temperature of the focal area exceeds 25°C ± 2°C, the power pump 10 will automatically increase the flow rate to 800 mL / min and restore the constant temperature within 30 seconds; an optical sensor is used to record the cell disruption rate. If it does not reach 90%, the ultrasound time will be automatically extended by 5 minutes or the transducer input voltage will be adjusted to 3000V AC to enhance the energy.

[0044] In application scenario verification, biological cell disruption experiments showed shorter disruption time and significantly higher disruption rates compared to traditional devices, enhanced intracellular protein extraction purity, and a constant temperature design that prevented protein inactivation. Test tube cleaning tests for micro-injection vials of specific specifications showed shorter cleaning times, higher removal rates, and complete removal of sidewall adhesions. Performance indicators demonstrated a high ultrasonic energy conversion rate, increased energy density in the focused area, and reduced energy consumption. The device demonstrated strong stability during continuous operation and a high cell disruption reproducibility. Its modular components allow for quick replacement of grooves of different specifications, ensuring strong compatibility.

Claims

1. A focused ultrasonic crushing / cleaning device based on an acoustic superstructure, characterized in that: The invention comprises a cylindrical groove (9) and a power pump (10), wherein an acoustic superstructure groove (6) is installed inside the cylindrical groove (9), and the acoustic superstructure groove (6) and the power pump (10) are connected via an installed coolant delivery pipe (11).

2. The energy-focusing ultrasonic crushing / cleaning device based on an acoustic superstructure according to claim 1, characterized in that: A test tube groove (8) is installed inside the acoustic superstructure groove (6), and a biological cell solution test tube (7) is installed in the test tube groove (8).

3. The energy-focusing ultrasonic crushing / cleaning device based on acoustic superstructure according to claim 1, characterized in that: Aluminum plate 2 (5) and aluminum plate 1 (1) are respectively installed at the bottom end of the cylindrical groove (9), and piezoelectric plate 3 (4), piezoelectric plate 2 (3), and piezoelectric plate 1 (2) are installed between the aluminum plate 2 (5) and aluminum plate 1 (1).

4. The energy-focusing ultrasonic crushing / cleaning device based on an acoustic superstructure according to claim 1, characterized in that: The acoustic superstructure groove (6) is made of aluminum and is adapted to the specific shape of the biological cell solution test tube (7).

5. The energy-focusing ultrasonic crushing / cleaning device based on acoustic superstructure according to claim 1, characterized in that: The power pump (10) is a peristaltic pump without valves and impellers.

6. The energy-focusing ultrasonic crushing / cleaning device based on acoustic superstructure according to claim 1, characterized in that: The cooling liquid in the cooling liquid delivery pipe (11) is ionized water, and the temperature is controlled at 25°C.

7. The energy-focusing ultrasonic crushing / cleaning device based on acoustic superstructure according to claim 1, characterized in that: The device also includes a high-power ultrasonic transducer, whose input voltage range is set to 1500-8000VAC. After conversion by the power conversion module, it can output a high-frequency electrical signal of 40-120kHz.

8. The energy-focusing ultrasonic crushing / cleaning device based on acoustic superstructure according to claim 3, characterized in that: The piezoelectric sheet three (4), the piezoelectric sheet two (3) and the piezoelectric sheet one (2) are mounted on the aluminum plate two (5) and the aluminum plate one (1) by welding, and are connected to an external power drive circuit; The piezoelectric piece three (4), the piezoelectric piece two (3) and the piezoelectric piece one (2) can stably generate a high-frequency electrical signal of 20-40kHz when driven by a voltage of 5-20VDC.

9. The energy-focusing ultrasonic crushing / cleaning device based on acoustic superstructure according to claim 7, characterized in that: The high-power ultrasonic transducer, the acoustic superstructure groove (6), and the coolant delivery pipe (11) in the device are all independent modular components and can be quickly disassembled and replaced according to experimental requirements.

10. The energy-focusing ultrasonic crushing / cleaning device based on acoustic superstructure according to claim 1, characterized in that: The device adopts an array-type multi-slot structure design, which can accommodate multiple biological test tubes for parallel experiments at the same time, and optimizes the acoustic isolation design between holes.

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