Freeze-dried cat food capable of improving immune function of cats

Abstract

The invention discloses a cat food freeze-dried food capable of improving the immune function of cats. The cat food freeze-dried food is characterized by being prepared from 1-5 parts of agaricus blazei murill polysaccharide; 40 parts of chicken breast; 20 parts of chicken thigh meat; 15 parts of chicken liver; 10 parts of eggs; and 10 parts of goat milk powder. The addition of the agaricus blazei murill polysaccharide is beneficial for improving the fur state, liver function and immune function of cats, and the immune improvement effect of a 600mg / kg addition group is the best, so that the optimal addition amount of the agaricus blazei murill polysaccharide recommended by the test in the cat food is 600mg / kg.

Description

Technical Field

[0001] The invention belongs to the technical field of pet feed, and mainly relates to a cat food product that improves the immune function of a cat. Background Art

[0002] Pet food is a high-end animal food that is between human food and traditional livestock and poultry feed and carries human emotions. It is food specially provided for pets and is an essential commodity in the process of pet raising.

[0003] Functional pet foods, by adding specific nutritionally active ingredients, address pets' specific nutritional needs and improve their health, regulating their body functions. Functional pet foods can be complete pet foods, supplements, or treats. They possess three key characteristics: safety and controllability, targeted functionality, and nutritional supplementation. Safety and controllability mean that through scientific formulation, these products ensure pets' tolerance within the recommended dosage range, avoiding the risk of toxic side effects associated with conventional feeding. Targeted functionality refers to products developed based on pets' specific physiological needs. These products utilize functional ingredients (such as omega-3 fatty acids for coat health, prebiotics for gut microbiome regulation, and amino acids and glucose for joint function) to achieve preventive health benefits or assist in improving specific physiological parameters. Nutritional supplementation distinguishes them from veterinary therapeutic drugs and falls under the category of dietary supplements. According to regulatory requirements, functional pet foods must not claim to treat diseases and must clearly display warnings such as "Does not replace professional veterinary care" in the product description to ensure compliance with the "Regulations on the Administration of Pet Food Labeling."

[0004] Functional pet foods can be divided into the following categories based on their functions: (1) pet foods with immune regulation function; (2) pet foods with intestinal health regulation function; (3) pet foods with antioxidant and anti-aging functions; (4) pet foods with hair health improvement function; (5) pet foods with weight regulation function; and (6) pet foods with stress relief and mood regulation function.

[0005] Currently, pet food research is relatively well-developed both domestically and internationally, and research on basic cat diets has reached maturity. Therefore, the development of functional pet foods has become a major development direction for pet food. Developing new pet food formulas and improving cats' immune function and health through nutritional regulation is of great significance. Summary of the Invention

[0006] The present invention discloses a freeze-dried cat food for improving the immune function of cats, which is characterized by being composed of the following raw materials in parts by weight: 0.06-0.1 portion of Agaricus blazei polysaccharide; 10-40 portions of chicken breast; 10-40 servings of chicken thighs; 10-40 servings of chicken livers; 1-10 parts eggs; 1-10 parts goat milk powder.

[0007] The present invention further discloses the use of a freeze-dried cat food for improving the immune function of cats. The immune indicators described are: improving the condition of a cat's fur and immune function. Specifically, these indicators include indicators of some anti-inflammatory and anti-inflammatory factors, immunoglobulin levels, CD4, CD8, and lymphocyte activity. Experimental results showed that Agaricus blazei Murrill polysaccharide is beneficial for improving the condition of a cat's fur and immune function, with the 600 mg / kg addition group showing the greatest immune-boosting effect. Therefore, this study recommends that the optimal addition amount of Agaricus blazei Murrill polysaccharide to cat food is 600 mg / kg.

[0008] The more detailed preparation method of the present invention is as follows: Technical solution: Add different amounts of Agaricus blazei murill polysaccharide to cat food products, collect samples after feeding the animals, and test the changes in fur condition, inflammatory factors, immune indicators and lymphocyte activity during the trial period. Based on the test results, determine the impact of Agaricus blazei murill polysaccharide on the immune function and health level of cats and determine its optimal addition amount.

[0009] Beneficial effects: The new pet food formula promotes the improvement of cats' immune function and health level through nutritional regulation measures.

[0010] This study aims to address the lack of domestic functional pet food development and the shortage of pet food that improves pet immunity. The study focused on the effects of three different addition amounts of Agaricus blazei murill polysaccharide on the immune function and health of cats. The results showed that: (1) Adding Agaricus blazei polysaccharide significantly improved the brightness and smoothness of cat fur. P <0.01).

[0011] (2) Addition of Agaricus blazei murill polysaccharide significantly increased the IL-4 content in cat serum on day 42 (P < 0.01) and the IL-4, IgA, and IgG content in cat serum on day 63 (P < 0.01). Addition of 600 mg / kg Agaricus blazei murill polysaccharide significantly increased the IgA, IgM, and CD8 content in cat serum on day 21 and the CD8 content on day 63 (P < 0.05), and significantly increased the IgA, IgG, and IgM content on day 63 (P < 0.01). Addition of Agaricus blazei murill polysaccharide has a significant effect on improving the immune function of cats.

[0012] (3) During the experimental period, there was no significant difference in lymphocyte activity among the groups (P>0.05). However, the group supplemented with 600 mg / kg Agaricus blazei polysaccharide showed a steady upward trend (except for the 21st day when all groups showed a significant decrease) and was higher than the other groups.

[0013] In summary, under the conditions of this study, Agaricus blazei murill polysaccharide can improve the coat condition and immune function of cats. The 600 mg / kg group showed the greatest immune-boosting effect. Therefore, this study recommends that the optimal dosage of Agaricus blazei murill polysaccharide in cat food be 600 mg / kg. The main difficulty lies in the limited sampling, which limits the number of sample types that can be analyzed. DETAILED DESCRIPTION

[0014] The present invention is described below by way of specific embodiments. Unless otherwise specified, the technical means used in the present invention are methods well known to those skilled in the art. In addition, the embodiments should be understood to be illustrative rather than limiting the scope of the present invention, the spirit and scope of the present invention being limited only by the claims. For those skilled in the art, various changes or modifications to the material components and dosages in these embodiments, without departing from the spirit and scope of the present invention, also fall within the scope of protection of the present invention. The raw materials and reagents used in the present invention are all commercially available. Example 1

[0015] A cat food for improving the immune function of a cat comprises the following components in parts by weight: 30 parts of chicken breast meat, 30 parts of chicken thigh meat, 30 parts of chicken liver, 5 parts of eggs, 4 parts of goat milk powder, 1 part of premix, and 0.06 parts of Agaricus blazei murill polysaccharide. Example 2

[0016] See Table 1:

[0017] Conclusion: Agaricus blazei murill polysaccharide is beneficial to improving the hair score and immune function of cats. The 600 mg / kg group showed the best immune-enhancing effect. Therefore, this study recommends that the optimal addition amount of Agaricus blazei murill polysaccharide in cat food is 600 mg / kg. Example 3

[0018] 1. Experimental Design A single-factor design was used to select 30 healthy adult cats (average age 2.00 ± 0.50 years and average weight 3.70 ± 0.02 kg). The cats were divided into five groups: control group 1 (C1) was fed a basal diet supplemented with a basal freeze-dried diet; control group 2 (C2) was fed a basal diet supplemented with a control freeze-dried diet; a low-dose group (T1) was fed a basal diet supplemented with 600 mg / kg freeze-dried Agaricus blazei Murrill polysaccharide; a medium-dose group (T2) was fed a basal diet supplemented with 800 mg / kg freeze-dried Agaricus blazei Murrill polysaccharide; and a high-dose group (T3) was fed a basal diet supplemented with 1000 mg / kg freeze-dried Agaricus blazei Murrill polysaccharide. Each group had six replicates, with one cat per replicate. The experimental period lasted 73 days, including a 10-day pre-test period and a 63-day test period.

[0019] 1.1 Sources Agaricus blazei polysaccharide (brown yellow powder, content 50%): commercially available from Xi'an Pinhua Biotechnology Co., Ltd. 1.2 Feeding and management The basal diet was prepared according to the cat feeding standard "GB / T 31217-2014." All groups were fed the same basal diet throughout the experimental period, with a basal diet to experimental freeze-dried feed ratio of approximately 8.9:1. The basal diet formula and nutritional levels are shown in Table 1-1.

[0020] Before the experiment began, the cages, feed bowls, and litter boxes were rinsed and disinfected. Deworming and vaccinations were completed according to the normal immunization schedule. The cattery was cleaned and disinfected daily. Throughout the experiment, cats were fed the corresponding freeze-dried food in their pens at 9:00 AM each day. Cat food was fed at designated times and locations daily at 9:00 AM and 2:00 PM. Water was freely available throughout the day.

[0021] 1.3 Data Analysis The experimental data were analyzed by one-way analysis of variance using SPSS 27.0 software and the ANOVA procedure was used for one-way analysis of variance. The results are expressed as "mean ± standard error." P>0.05 indicates no significant difference, P<0.05 indicates a significant difference, and P<0.01 indicates an extremely significant difference.

[0022] 1.4 Composition and nutritional content of basic feed and basic freeze-dried formula Table 1-1 Composition and nutritional level of basic feed and basic freeze-dried formula (air-dried basis)

[0023] Note: The premix provides per kilogram of feed: VA 5000 IU, VD 500 IU, VE 30 IU, VK 0.1 mg, VB15 mg, VB2 4 mg, VB5 5 mg, VB3 60 mg, VB6 4 mg, folic acid 0.8 mg, biotin 0.07 mg, VB120.02 mg, choline 2400 mg, iron 80 mg, copper 10 mg, manganese 7.5 mg, zinc 75 mg, iodine 0.35 mg, and selenium 0.1 mg.

[0024] 2. Test results 2.1 Effect of Agaricus blazei polysaccharide on cat fur color and brightness As shown in Table 2-1, on the 63rd day of the experiment, the hair color brightness of groups T1, T2, and T3 was significantly higher than that of group C2 (P < 0.01).

[0025] Table 2-1 Effects of Agaricus blazei polysaccharide on cat fur color and brightness

[0026] Note: Data in the same row with different lowercase letters indicate significant differences between groups ( P <0.05), different capital letters indicate extremely significant differences between groups ( P <0.01), the same as in the table below.

[0027] 2.2 Effect of Agaricus blazei polysaccharide on cat hair smoothness As shown in Table 2-2, on the 63rd day of the experiment, the hair smoothness of groups C1 and C2 was significantly improved by groups T1, T2 and T3 ( P＜ 0.01).

[0028]

[0029] 2.3 Effects of Agaricus blazei polysaccharide on inflammatory factors in cat serum 2.3.1 Determination of the effect of adding Agaricus blazei murill polysaccharide on immune factors in cat serum On the first day of the experiment, there was no significant difference in inflammatory factors among the groups ( P＞ 0.05).

[0030] On the 21st day of the experiment, IL-6 in the T1 and C2 groups was significantly higher than that in the T2 and T3 groups ( P <0.05).

[0031] On the 42nd day of the experiment, the IL-4 levels in the T1 and C2 groups were significantly higher than those in the T3 and C1 groups, while the IL-4 levels in the C1 group were significantly lower than those in the T1, T2, T3, and C2 groups ( P <0.05); IL-10 in T1 and T2 groups was significantly higher than that in T3 and C2 groups, while IL-10 in T3 group was significantly lower than that in T1, T2 and C1 groups ( P <0.05).

[0032] On the 63rd day of the experiment, IL-1β in the C2 and T2 groups was significantly higher than that in the C1, T1, and T3 groups, while that in the T1 group was significantly lower than that in the T2, T3, and C2 groups ( P <0.01); IL-4 in T2 group was significantly higher than that in T1, T3, C1 and C2 groups, while that in C1 group was significantly lower than that in T1, T2, T3 and C2 groups ( P <0.05); IL-6 in the C2 group was significantly higher than that in T1, T3, and C1, and significantly lower than that in the T1 and C1 groups ( P <0.05); IL-10 in the T2 group was significantly higher than that in the T1, T3, and C2 groups, and that in the T1 group was significantly lower than that in the T2, C1, and C2 groups ( P <0.05); TNF-α in T2 group was significantly higher than that in T1, T3 and C1 groups, while that in C1 group was significantly lower than that in T1, T2 and C2 groups ( P <0.05); IFN-γ in T2 group was significantly higher than that in T1, T3, C1 and C2 groups, while that in C1 group was significantly lower than that in T2, T3 and C2 groups (P <0.05).

[0033] Table 2-3 Effects of Agaricus blazei polysaccharide on the levels of inflammatory factors in cat serum

[0034]

[0035] 2.4 Effects of Agaricus blazei polysaccharide on immune factors in cat serum 2.4.1 Determination of the effect of adding Agaricus blazei murill polysaccharide on immune factors in cat serum On the first day of the experiment, there was no significant difference in immune factors among the groups ( P＞ 0.05).

[0036] On the 21st day of the experiment, IgA in the T2 group was significantly higher than that in the T3 and C2 groups, and that in the T3 group was significantly lower than that in the T1, T2, and C2 groups ( P <0.05); IgM in T1, T2, and C1 groups was significantly higher than that in T3 and C2 groups ( P <0.05).

[0037] On the 42nd day of the experiment, the IgA in the T1 and T2 groups was significantly higher than that in the T3, C1, and C2 groups, and the IgA in the C2 group was significantly lower than that in the T1, T2, T3, and C1 groups ( P <0.05); IgG in T2 group was significantly higher than that in T1 and T3 groups ( P <0.05); IgM in T1, T3, and C2 groups was significantly higher than that in T2 and C1 groups ( P <0.05); CD4 in T3 group was significantly higher than that in T1, T2, C1, and C2 groups ( P <0.05); CD8 in T1 and T3 groups was significantly higher than that in T2, C1, and C2 groups ( P <0.05).

[0038] On the 63rd day of the experiment, the IgA level in group C2 was significantly higher than that in groups T1, T3, and C1, while that in group C1 was significantly lower than that in groups T1, T2, T3, and C2 ( P <0.05); IgG in the T1 group was significantly higher than that in the T2, T3, C1, and C2 groups, while that in the C1 group was significantly lower than that in the T1, T2, T3, and C2 groups ( P <0.05); IgM in T1 and T2 groups was significantly higher than that in T3, C1, and C2 groups ( P <0.05); CD4 in T2 and C2 groups was significantly higher than that in C1 group ( P <0.05); CD8 in group C2 was significantly higher than that in groups T3 and C1, while that in group C1 was significantly lower than that in groups T1 and C2 (P<0.05).

[0039] Table 2-4 Effects of Agaricus blazei polysaccharide on immune factors in cat serum

[0040] 2.5 Effect of Adding Agaricus Blazei Polysaccharide on Feline Lymphocyte Activity During the experimental period, there was no significant difference in lymphocyte activity among the groups ( P＞ 0.05). However, the C1, T1, and T2 groups showed a steady upward trend (except for the 21st day when all groups decreased significantly). The T1 group was higher than the other groups.

[0041]

[0042] 3. Experimental Summary 3.1 Effect of Agaricus blazei polysaccharide on cat hair score Adding Agaricus blazei polysaccharide significantly improves the brightness and smoothness of cat's fur. P＜ 0.01).

[0043] 3.2 Effects of Agaricus blazei polysaccharide on inflammatory factors in cat serum Adding Agaricus blazei polysaccharide significantly increased the IL-4 content in cat serum on day 42 ( p＜ 0.01), IL-4, IgA, and IgG levels in cat serum on the 63rd day ( p＜ 0.01).

[0044] 3.3 Effects of Agaricus blazei polysaccharide on immune factors in cat serum Adding 600 mg / kg Agaricus blazei polysaccharide significantly increased the levels of IgA, IgM, and CD8 in cat serum on day 21 and the level of CD8 on day 63 ( p <0.05), and significantly increased the levels of IgA, IgG, and IgM on the 63rd day ( p＜ 0.01). Adding Agaricus blazei murill polysaccharide can significantly improve the immune function of cats.

[0045] 3.4 Effect of Adding Agaricus Blazei Polysaccharide on Feline Lymphocyte Activity (1) During the experimental period, there was no significant difference in lymphocyte activity among the groups (P>0.05).

[0046] (2) The group supplemented with 600 mg / kg Agaricus blazei polysaccharide showed a steady upward trend (except for the 21st day when all other groups decreased significantly) and was higher than the other groups.

[0047] 4. Conclusion (1) This study showed that the addition of Agaricus blazei polysaccharide improved the brightness and smoothness of the cats' fur, and had a positive effect on the condition of the cats' fur.

[0048] (2) Adding Agaricus blazei polysaccharide increased the content of immune factors in cat serum, which helped them to make immune responses in a timely manner. Among them, adding 600 mg / kg Agaricus blazei polysaccharide had the best effect.

[0049] (3) The lymphocyte activity of the group supplemented with 600 mg / kg Agaricus blazei polysaccharide was higher than that of the other groups, indicating good immune ability.

[0050] In summary, under the conditions of this experiment, Agaricus blazei murill polysaccharide is beneficial to improving the fur condition, liver function and immune function of cats. Among them, the 600 mg / kg addition group has the best immune enhancement effect. Therefore, this experiment recommends that the optimal addition amount of Agaricus blazei murill polysaccharide in cat food is 600 mg / kg.

Claims

1. A freeze-dried cat food for improving the immune function of cats, characterized in that The invention is composed of the following raw materials in parts by weight: 29.94 parts of chicken breast meat, 30 parts of chicken thigh meat, 30 parts of chicken liver, 5 parts of eggs, 4 parts of goat milk powder, 1 part of premix and 0.06 part of Agaricus blazei polysaccharide.

2. Use of the freeze-dried cat food for improving cat immune function according to claim 1 for improving cat immunity; the cat immune function refers to: improving the cat's fur condition and liver function.

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