High-efficiency body function repairing active small molecule peptide preparation and preparation method thereof

Abstract

The invention relates to the technical field of biological preparations, and discloses an efficient body function repairing active small molecule peptide preparation and a preparation method thereof.The efficient body function repairing active small molecule peptide preparation is prepared from 15-22 parts of collagen peptide, 5-9 parts of glutathione, 10-18 parts of whey protein peptide, 5-8 parts of vitamin C, 2-4 parts of vitamin E, 1-3 parts of zinc element, 5-15 parts of astragalus membranaceus extract, 3-8 parts of probiotics and 10-20 parts of a delivery carrier. The molecular weight range of the original protein peptide, the glutathione and the whey protein peptide is 500 to 2000Da. According to the invention, collagen peptide, glutathione, whey protein peptide, vitamins and other components are proportioned to realize an anti-oxidation-repair-anti-inflammatory synergistic effect, and vitamin C, vitamin E, zinc element and an astragalus extract are added to cover multi-dimensional repair of an immune system, so that comprehensive body function repair is realized.

Description

Technical Field

[0001] The present invention relates to the technical field of biological preparations, and in particular to a high-efficiency active small molecule peptide preparation for repairing body functions and a preparation method thereof. Background Art

[0002] As living standards improve, people's interest in physical health and functional recovery is growing. Whether due to daily fatigue, sports injuries, or aging-related decline in physical function, people yearn for effective products to promote recovery. Amidst the rapid development of biotechnology, small molecule peptides have garnered widespread attention due to their unique bioactivity and excellent absorbability. Currently, peptide preparations on the market primarily focus on single-function repairs for joint pain and inflammation.

[0003] However, the existing high-efficiency active small molecule peptide preparations for repairing body functions still have many shortcomings. Their ingredients are single, making it difficult to synergistically promote the repair of body functions in multiple dimensions. They do not fully consider the relationship between body functions, resulting in limited effects on repairing and improving the overall body functions. Summary of the Invention

[0004] In response to the shortcomings of the existing technology, the present invention provides an efficient small molecule peptide preparation for repairing body functions and a preparation method thereof, which solves the problems that the existing peptide preparations have a single component, are difficult to promote the repair of body functions in a multi-dimensional synergistic manner, and have limited effects on repairing and improving the overall body functions.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: a highly efficient small molecule peptide preparation for repairing body functions, comprising: 15-22 parts of collagen peptide, 5-9 parts of glutathione, 10-18 parts of whey protein peptide, 5-8 parts of vitamin C, 2-4 parts of vitamin E, 1-3 parts of zinc, 5-15 parts of astragalus extract, 3-8 parts of probiotics, and 10-20 parts of delivery carrier.

[0006] Preferably, the molecular weight of the proprotein peptide, glutathione and whey protein peptide is in the range of 500-2000 Da.

[0007] Preferably, the astragaloside content in the astragalus extract is ≥0.5%.

[0008] Preferably, the probiotics are a composite strain of Lactobacillus plantarum and Lactobacillus rhamnosus, with a mass ratio of 1:1-2:1, and the probiotics are encapsulated with trehalose, with an encapsulation rate of ≥90%.

[0009] Preferably, the delivery vehicle comprises enteric-coated microspheres, the enteric-coated material is acrylic resin, the particle size range is 100-300 μm, and the release rate is ≥95% in an environment of pH ≥6.8.

[0010] A method for preparing a highly effective small molecule peptide preparation for restoring bodily functions, the method comprising the following steps:

[0011] S1, enzymatic immobilization, using immobilized enzyme technology to co-immobilize trypsin and flavor protease on magnetic nanoparticles;

[0012] S2. Preparation of active peptides: animal collagen raw materials and plant protein raw materials are subjected to ultrasonic-assisted defatting, crushed to 80-120 mesh, and immobilized trypsin and flavor protease are added for enzymatic hydrolysis;

[0013] S3, Astragalus extract treatment, after crushing the Astragalus, reflux extraction with 60-80% ethanol 2-3 times, the extracts are combined, concentrated under reduced pressure until there is no alcohol taste, and spray dried to obtain powder;

[0014] S4, probiotic treatment, centrifuging the probiotic fermentation broth to collect the bacteria, mixing it with the trehalose solution, and preparing the embedded bacterial powder by spray drying or freeze drying;

[0015] S5. Formulation: Active peptides, vitamin-zinc complex, astragalus extract, probiotics and delivery carrier are mixed and made into enteric-soluble microspheres through fluidized bed coating.

[0016] Preferably, the preparation of the S2 active peptide specifically comprises the following steps:

[0017] S201, defatting the animal collagen raw material and the plant protein raw material with the aid of ultrasonic wave, and crushing the raw material into 80-120 mesh;

[0018] S202, immobilized trypsin and flavor protease were hydrolyzed at pH 7.5 and 50 °C for 5 h, and then immobilized on Fe3O4@SiO2-NH2 magnetic nanoparticles by glutaraldehyde cross-linking;

[0019] S203, shaking the immobilized trypsin and flavor protease with the crushed protein raw material in an environment of pH 7.5-8.0 and temperature 50-55° C. for 2-6 hours;

[0020] S204, collecting peptides with a molecular weight of 500-2000 Da through ultrafiltration membrane interception, separating the immobilized enzyme through a magnetic field strength of 0.3-0.5 T, washing the immobilized enzyme with a pH 7.0 buffer solution to remove the adsorbed peptides, and recovering the immobilized enzyme.

[0021] Preferably, the S5 preparation molding specifically includes the following steps:

[0022] S501, dissolving the active peptide, vitamin-zinc complex, astragalus extract and acrylic resin in a mixed solvent of acetone and water;

[0023] S502, using spray drying method to form pellets, with inlet temperature of 80-90°C and outlet temperature of 40-50°C;

[0024] S503, the microspheres and the probiotic powder are mixed by a dry method.

[0025] The present invention provides a highly effective small molecule peptide preparation for restoring bodily functions and a preparation method thereof. It has the following beneficial effects:

[0026] 1. The present invention achieves a synergistic effect of antioxidant, repair and anti-inflammatory by matching ingredients such as collagen peptides, glutathione, whey protein peptides and vitamins, and by adding vitamins C, E, zinc and astragalus extract, it covers the multi-dimensional repair of the immune system and achieves comprehensive body function repair.

[0027] 2. The present invention achieves efficient reuse of enzymes by co-immobilizing trypsin and flavor protease on magnetic nanoparticles, and greatly improves the activity retention rate after repeated use. The enzymatic hydrolysis process is precisely controllable, and proteins are directionally cut to generate active small molecule peptides, significantly reducing production costs.

[0028] 3. The present invention uses acrylic resin to prepare microspheres with a particle size, embeds active peptides, astragalus extracts, etc., and combines dry mixing of probiotic powder to protect the ingredients from gastric acid degradation, thereby improving the accuracy of intestinal targeted release, improving bioavailability, and reducing waste of active ingredients and side effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 The present invention is a flow chart of the method for preparing the highly effective small molecule peptide preparation for repairing body functions. DETAILED DESCRIPTION

[0030] The following will clearly and completely describe the technical solution of the present invention in conjunction with the accompanying drawings. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0031] Please see the attached Figure 1 The embodiment of the present invention provides a high-efficiency body function repair active small molecule peptide preparation and a preparation method thereof, including: 15-22 parts of collagen peptide, 5-9 parts of glutathione, 10-18 parts of whey protein peptide, 5-8 parts of vitamin C, 2-4 parts of vitamin E, 1-3 parts of zinc, 5-15 parts of astragalus extract, 3-8 parts of probiotics, and 10-20 parts of delivery carrier.

[0032] Specifically, collagen peptides, which are rich in amino acids such as glycine and proline, can supplement the body's essential amino acid precursors, promote the synthesis of collagen by skin fibroblasts, enhance skin elasticity, reduce wrinkles, and improve moisturizing ability. At the same time, they can stimulate chondrocytes to secrete proteoglycans, relieve joint pain and improve mobility. When working synergistically with whey protein peptides, they can activate muscle repair pathways and accelerate tissue regeneration.

[0033] Glutathione, as an endogenous antioxidant, directly neutralizes free radicals through sulfhydryl groups (-SH), regenerates the antioxidant capacity of vitamin C / E, and participates in the liver detoxification process. It can reduce oxidative stress markers in the body (such as a 50% decrease in MDA levels), improve liver function indicators (ALT / AST decreases by 35%), and delay UV-induced skin photoaging.

[0034] Whey protein peptides are rich in branched-chain amino acids (BCAAs), which promote muscle protein synthesis by activating the mTOR pathway and inhibiting NF-κB-mediated inflammatory responses, thereby reducing muscle damage markers (creatine kinase) by 45% within 24 hours after exercise, reducing CRP levels in patients with chronic inflammation by 30%, and improving endurance performance (VO2max increased by 12%).

[0035] Vitamin C is a water-soluble antioxidant that can scavenge free radicals and regenerate glutathione. It participates in the collagen hydroxylation process as a cofactor, stabilizes the collagen fiber structure, and, in combination with collagen peptides, shortens wound healing time by 25%, increases the ultraviolet protection factor (SPF) by 2 times, and enhances iron absorption (Hb increases by 20% in anemic patients).

[0036] Vitamin E is a fat-soluble antioxidant that embeds into cell membranes to prevent lipid peroxidation, inhibits the release of pro-inflammatory factors such as prostaglandin E2 (PGE2), reduces the intensity of delayed onset muscle soreness (DOMS) after exercise, and improves transdermal absorption rate when combined with a liposome delivery system.

[0037] Zinc is a component and activator of many enzymes. It participates in the synthesis of proteins and nucleic acids, as well as cell division and growth. It plays an important role in maintaining the body's normal immune function, wound healing, and sense of taste and smell. It enhances immunity, promotes wound healing, improves appetite, and maintains normal growth and development. It also has a certain promoting effect on skin health and helps prevent skin diseases such as acne.

[0038] Astragalus extract contains a variety of active ingredients, such as astragalus polysaccharides and astragaloside, which have multiple pharmacological effects such as immunomodulation, antioxidant, anti-inflammatory, and lipid-lowering. At the same time, it can enhance the body's immune function, improve the body's stress resistance, and promote the body's metabolism.

[0039] Probiotics can regulate the balance of intestinal microecology, inhibit the growth of harmful bacteria, promote the reproduction of beneficial bacteria, enhance intestinal barrier function, improve intestinal digestion and absorption function, and regulate the body's immune response by interacting with the immune system;

[0040] The delivery vehicle uses enteric-coated microspheres to protect the active ingredients from being destroyed by gastric acid, achieving intestinal targeted release or transdermal sustained release, ensuring that the various active ingredients can effectively function in the body and improving the efficacy of the product; reducing the waste of active ingredients, lowering the required dosage, and thus reducing possible side effects.

[0041] The molecular weights of the proprotein peptide, glutathione and whey protein peptide are in the range of 500-2000 Da.

[0042] Specifically, collagen peptides, glutathione and whey protein peptides with a molecular weight range of 500-2000Da exert repair, antioxidant and anti-inflammatory functions respectively through efficient absorption and targeting. Among them, collagen peptides stimulate collagen synthesis, increase skin moisture content by 30% and accelerate wound healing. Glutathione scavenges free radicals, reduces oxidative stress markers (such as MDA) by 50%, and protects liver function. Whey protein peptides promote muscle synthesis, reduce post-exercise muscle damage by 40%, and inhibit chronic inflammation (CRP levels drop by 30%). The three synergistically form an antioxidant-repair-anti-inflammatory network, significantly enhancing the repair and protection capabilities of the skin, joints, muscles and immune system.

[0043] The astragaloside content in the astragalus extract is ≥0.5%.

[0044] Specifically, astragalus extract activates the TLR4 / NF-κB signaling pathway, enhances NK cell activity and mitochondrial ATP synthesis, improves the body's disease resistance, and promotes intestinal sIgA secretion and improves intestinal barrier function. Existing clinical data show that astragalus extract can reduce the fatigue index of chronic fatigue patients by 50%, reduce the incidence of upper respiratory tract infections by 60% during the flu season, and significantly enhance intestinal immunity with probiotics.

[0045] The probiotics are composite bacteria of Lactobacillus plantarum and Lactobacillus rhamnosus, with a mass ratio of 1:1-2:1. The probiotics are embedded in trehalose, and the embedding rate is ≥90%.

[0046] Specifically, the probiotics use a composite strain of Lactobacillus plantarum and Lactobacillus rhamnosus. After trehalose encapsulation treatment, the encapsulation rate is ≥90%, ensuring its high survival rate in the gastric acid environment. It strengthens the intestinal barrier function by secreting short-chain fatty acids (SCFAs), regulates the balance of intestinal flora, and affects the synthesis of neurotransmitters (such as 5-HT) through the gut-brain axis, thereby improving intestinal and nervous system health and reducing the incidence of antibiotic-associated diarrhea.

[0047] The delivery carrier comprises enteric-coated microspheres, the enteric-coated material is acrylic resin, the particle size range is 100-300 μm, and the release rate is ≥95% in an environment of pH ≥6.8.

[0048] Specifically, the delivery carrier adopts enteric microsphere technology, with acrylic resin as the enteric material and a particle size range of 100-300μm, ensuring a release rate of ≥95% in an intestinal environment with a pH ≥ 6.8. It protects active ingredients (such as peptides and probiotics) from gastric acid degradation, achieves precise intestinal targeted release, significantly improves bioavailability, and is suitable for intestinal targeted repair and immune regulation of oral preparations.

[0049] A method for preparing a highly effective small molecule peptide preparation for restoring bodily functions, the method comprising the following steps:

[0050] S1, enzymatic immobilization, using immobilized enzyme technology to co-immobilize trypsin and flavor protease on magnetic nanoparticles;

[0051] S2. Preparation of active peptides: animal collagen raw materials and plant protein raw materials are subjected to ultrasonic-assisted defatting, crushed to 80-120 mesh, and immobilized trypsin and flavor protease are added for enzymatic hydrolysis;

[0052] S3, Astragalus extract treatment, after crushing the Astragalus, reflux extraction with 60-80% ethanol 2-3 times, the extracts are combined, concentrated under reduced pressure until there is no alcohol taste, and spray dried to obtain powder;

[0053] S4, probiotic treatment, centrifuging the probiotic fermentation broth to collect the bacteria, mixing it with the trehalose solution, and preparing the embedded bacterial powder by spray drying or freeze drying;

[0054] S5. Formulation: Active peptides, vitamin-zinc complex, astragalus extract, probiotics and delivery carrier are mixed and made into enteric-soluble microspheres through fluidized bed coating.

[0055] Specifically, in S1, trypsin and flavor protease are co-immobilized on magnetic nanoparticles through immobilized enzyme technology, achieving efficient reuse and directional cleavage of the enzymes. The enzyme activity retention rate can be ≥80% after being reused ≥10 times, significantly reducing production costs.

[0056] In S2, the animal collagen and plant protein raw materials are subjected to ultrasonic-assisted defatting treatment to remove impurities such as oil in the raw materials to prevent them from adversely affecting the subsequent enzymatic hydrolysis reaction and product quality. The raw materials are then crushed to 80-120 mesh to increase the specific surface area of ​​the raw materials, which is conducive to sufficient contact between the enzyme and the substrate. Immobilized trypsin and flavor protease are added for enzymatic hydrolysis. The two enzymes work synergistically to hydrolyze the proteins into small-molecule peptides with multiple physiological activities according to specific enzymatic cleavage sites. The small-molecule peptides have good biological activity and absorbability, and can promote the enhancement of skin elasticity, repair of joint cartilage, regeneration of muscle tissue and other body function repair effects.

[0057] In S3, the astragalus root is crushed and then reflux-extracted with 60-80% ethanol for 2-3 times to fully extract the various active ingredients in the astragalus root, such as astragalus polysaccharides and astragalus saponins. The extracts are combined and concentrated under reduced pressure until there is no alcohol taste, thereby removing the ethanol solvent in the extracts and retaining the active ingredients. The powder is then spray-dried to facilitate mixing and molding in the subsequent preparation process and is also beneficial for storage and transportation of the product.

[0058] In S4, the probiotic fermentation broth is centrifuged to collect the bacteria to obtain a high concentration of probiotic bacteria, which are then mixed with a trehalose solution. Trehalose has a good protective effect and can stabilize the cell membrane and protein structure of the probiotics during the subsequent drying process. The embedded bacterial powder is prepared by spray drying or freeze drying, and the probiotics are wrapped in a certain carrier, thereby improving the survival rate of the probiotics in the harsh environment of the gastrointestinal tract, thereby ensuring the activity of the probiotics during product storage. After entering the human gastrointestinal tract, the probiotics can resist the erosion of gastric acid and bile, smoothly reach the intestine and play a role in regulating the balance of intestinal microecology, improving the digestion and absorption function of the intestine, enhancing the body's immunity, and thus promoting the repair and maintenance of the overall body function;

[0059] In S5, active peptides, vitamin-zinc complex, astragalus extract, probiotics and delivery carriers are mixed to evenly disperse the ingredients. Enteric-soluble microspheres are made through fluidized bed coating. The coating material of the enteric-soluble microspheres can remain intact in the gastric acid environment to prevent the active ingredients from being destroyed by gastric acid. When reaching the intestine, the coating dissolves and releases the active ingredients inside, achieving precise targeted release.

[0060] The preparation of the S2 active peptide specifically comprises the following steps:

[0061] S201, defatting the animal collagen raw material and the plant protein raw material with the aid of ultrasonic wave, and crushing the raw material into 80-120 mesh;

[0062] S202, immobilized trypsin and flavor protease were hydrolyzed at pH 7.5 and 50 °C for 5 h, and then immobilized on Fe3O4@SiO2-NH2 magnetic nanoparticles by glutaraldehyde cross-linking;

[0063] S203, shaking the immobilized trypsin and flavor protease with the crushed protein raw material in an environment of pH 7.5-8.0 and temperature 50-55° C. for 2-6 hours;

[0064] S204, collecting peptides with a molecular weight of 500-2000 Da through ultrafiltration membrane interception, separating the immobilized enzyme through a magnetic field strength of 0.3-0.5 T, washing the immobilized enzyme with a pH 7.0 buffer solution to remove the adsorbed peptides, and recovering the immobilized enzyme.

[0065] Specifically, the animal collagen raw materials and the plant protein raw materials are subjected to ultrasonic-assisted degreasing treatment by S201. The high-frequency vibration generated by the ultrasonic wave can effectively destroy the combination of oil and protein, promote the separation of oil from the raw materials, thereby removing oil and other impurities in the raw materials, and preventing the oil from interfering with the combination of enzyme and substrate in the subsequent enzymatic hydrolysis reaction, and having an adverse effect on product quality, such as reducing product purity and affecting product stability.

[0066] S202 utilizes the characteristics of two enzymes to perform preliminary enzymatic hydrolysis on the protein raw material. Under these conditions, trypsin and flavor protease can initially cut protein molecules according to their respective specific enzymatic cleavage sites, breaking down large molecular proteins into smaller peptide fragments. These are then fixed to magnetic nanoparticles through glutaraldehyde cross-linking. Glutaraldehyde, as a cross-linking agent, can form covalent bonds between the enzyme molecules and the amino groups on the surface of the magnetic nanoparticles, achieving enzyme immobilization. The magnetic nanoparticles not only provide a fixed carrier for the enzyme, but also give the immobilized enzyme the ability to be separated and recovered under the action of an external magnetic field.

[0067] When the immobilized trypsin and flavor protease are shaken with the crushed protein raw material by S203, it can promote the full contact and mixing of the enzyme and the substrate, so that the enzymatic hydrolysis reaction proceeds more comprehensively and deeply. At the same time, the immobilized enzyme continues to further enzymatically cleave the remaining protein and the smaller peptides produced by the initial hydrolysis, so as to obtain more small molecule peptides with specific physiological activities and a molecular weight range of 500-2000Da;

[0068] Through S204, the target peptides are collected by ultrafiltration membrane interception and the selectivity of the ultrafiltration membrane pore size is utilized, unreacted macromolecular proteins, enzymes and other impurities are removed, and the purity of the active peptide product is improved. At the same time, the immobilized enzyme is separated by a magnetic field strength of 0.3-0.5T, and then the immobilized enzyme is washed with pH 7.0 buffer to remove the peptides adsorbed on the surface of the immobilized enzyme, so that the immobilized enzyme can maintain a high activity and purity, and is easy to recover and reuse, thereby ensuring the activity and stability of the immobilized enzyme during multiple uses, reducing production costs, and improving the economy and sustainability of the entire preparation process.

[0069] The S5 preparation molding specifically includes the following steps:

[0070] S501, dissolving the active peptide, vitamin-zinc complex, astragalus extract and acrylic resin in a mixed solvent of acetone and water;

[0071] S502, using spray drying method to form pellets, with inlet temperature of 80-90°C and outlet temperature of 40-50°C;

[0072] S503, the microspheres and the probiotic powder are mixed by a dry method.

[0073] Specifically, the acetone-water mixed solvent in S501 is used to evenly disperse and dissolve the components, ensuring that the components can be fully mixed in the subsequent processing process to prepare for ball formation;

[0074] In step S502, the mixed solution is atomized into tiny droplets through a spray device and sprayed into a hot air flow. The high-temperature inlet hot air causes the acetone-water mixed solvent in the droplets to evaporate rapidly, while the active peptides, vitamin-zinc complex, astragalus extract, and acrylic resin dissolved therein gradually aggregate and solidify to form spherical particles, which is beneficial for protecting the active ingredients and improving their stability. At the same time, the shape of the microspheres also facilitates subsequent mixing with probiotic powder and the storage and administration of the product.

[0075] By mixing probiotics with previously prepared microspheres containing other active ingredients through S503, the product can have multiple functions and ensure that the probiotics can work synergistically with other active ingredients in the final product;

[0076] Example 1: Basic formula and process

[0077] formula

[0078] 18 parts of collagen peptides, 7 parts of glutathione, 14 parts of whey protein peptides;

[0079] 6 parts of vitamin C, 3 parts of vitamin E, 2 parts of zinc;

[0080] 10 parts of astragalus extract, 5 parts of probiotics, and 15 parts of delivery vehicle.

[0081] Craftsmanship

[0082] Enzyme immobilization: trypsin and flavor protease (1:2) were immobilized on Fe3O4@SiO2-NH2 particles with an enzyme loading of 20 mg / g;

[0083] Preparation of active peptides: ultrasonic-assisted defatting, hydrolysis at pH 7.5, 50°C for 5 hours;

[0084] Astragalus extract: 70% ethanol reflux extraction twice, spray drying;

[0085] Probiotic treatment: Lactobacillus plantarum and Lactobacillus rhamnosus (1:1), trehalose-encapsulated;

[0086] Preparation molding: fluidized bed coating, enteric-coated microspheres with a particle size of 200 μm.

[0087] Example 2: Optimizing the enzymatic hydrolysis process

[0088] formula

[0089] 20 parts of collagen peptides, 8 parts of glutathione, 16 parts of whey protein peptides;

[0090] Vitamin C 7 parts, Vitamin E 4 parts, Zinc 3 parts;

[0091] 12 parts of astragalus extract, 6 parts of probiotics, and 18 parts of delivery vehicle.

[0092] Craftsmanship

[0093] Enzymatic immobilization: trypsin + flavor protease (1:1.5) was immobilized on Fe3O4@SiO2-NH2 particles, with an enzyme loading of 25 mg / g;

[0094] Preparation of active peptides: ultrasonic + alkaline expansion pretreatment, hydrolysis at pH 8.0, 55°C for 6 hours;

[0095] Astragalus extract: 80% ethanol reflux extraction 3 times, spray drying;

[0096] Probiotic treatment: Lactobacillus plantarum + Lactobacillus rhamnosus (1.5:1), trehalose-encapsulated;

[0097] Preparation molding: fluidized bed coating, enteric-coated microspheres with a particle size of 150 μm.

[0098] Example 3: Efficient delivery system

[0099] formula

[0100] 22 parts of collagen peptides, 9 parts of glutathione, 18 parts of whey protein peptides;

[0101] 8 parts of vitamin C, 4 parts of vitamin E, 3 parts of zinc;

[0102] 15 parts of astragalus extract, 8 parts of probiotics, and 20 parts of delivery vehicle.

[0103] Craftsmanship

[0104] Enzymatic immobilization: trypsin + flavor protease (1:2) was immobilized on Fe3O4@SiO2-NH2 particles, with an enzyme loading of 22 mg / g;

[0105] Preparation of active peptides: ultrasound + collagenase-assisted pretreatment, hydrolysis at pH 7.8 and 52°C for 5.5 hours;

[0106] Astragalus extract: 75% ethanol reflux extraction twice, spray drying;

[0107] Probiotic treatment: Lactobacillus plantarum + Lactobacillus rhamnosus (2:1), trehalose-encapsulated;

[0108] Preparation molding: fluidized bed coating, enteric-coated microspheres with a particle size of 100 μm.

[0109] Table 1 is a comparison table of different embodiments

[0110]

[0111]

[0112] in:

[0113] Antioxidant activity DPPH (1,1-diphenyl-2-trinitrophenylhydrazine):

[0114] In Example 2, the antioxidant activity was significantly improved by optimizing the enzymatic hydrolysis process (alkaline expansion pretreatment and prolonged hydrolysis time).

[0115] Example 3: Collagenase-assisted pretreatment further enhanced the antioxidant activity

[0116] Probiotic survival rate and intestinal immunity:

[0117] Example 3 achieves the highest gastric acid survival rate and intestinal sIgA enhancement by adjusting the probiotic ratio and optimizing the embedding process.

[0118] Enteric release rate:

[0119] Example 3 uses a smaller enteric-coated microsphere particle size, which has the highest release rate and is suitable for efficient intestinal targeted delivery.

[0120] Example 1 is a basic solution and is suitable for conventional repair needs;

[0121] Example 2 can significantly improve the peptide yield and antioxidant activity;

[0122] Example 3 performs best in terms of probiotic survival rate and enteric release rate, and is suitable for rapid repair.

[0123] Each embodiment meets the needs of different application scenarios by adjusting the formula ratio and process parameters, reflecting the flexibility and innovative body function of the technology.

[0124] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. Highly effective small molecule peptide preparation for restoring body functions, characterized by: include: 15-22 parts of collagen peptide, 5-9 parts of glutathione, 10-18 parts of whey protein peptide, 5-8 parts of vitamin C, 2-4 parts of vitamin E, 1-3 parts of zinc, 5-15 parts of astragalus extract, 3-8 parts of probiotics, and 10-20 parts of delivery carrier.

2. The high-efficiency body function repair active small molecule peptide preparation according to claim 1, characterized in that: The molecular weights of the proprotein peptide, glutathione and whey protein peptide are in the range of 500-2000 Da.

3. The high-efficiency body function repair active small molecule peptide preparation according to claim 1, characterized in that: The astragaloside content in the astragalus extract is ≥0.5%.

4. The high-efficiency body function repair active small molecule peptide preparation according to claim 1, characterized in that: The probiotics are composite bacteria of Lactobacillus plantarum and Lactobacillus rhamnosus, with a mass ratio of 1:1-2:

1. The probiotics are embedded in trehalose, and the embedding rate is ≥90%.

5. The high-efficiency small molecule peptide preparation for restoring body functions according to claim 1, characterized in that: The delivery carrier comprises enteric-coated microspheres, the enteric-coated material is acrylic resin, the particle size range is 100-300 μm, and the release rate is ≥95% in an environment of pH ≥6.

8.

6. A method for preparing a highly effective small molecule peptide preparation for restoring body functions, characterized in that: The method for the high-efficiency body function repair active small molecule peptide preparation according to any one of claims 1 to 5 comprises the following steps: S1, enzymatic immobilization, using immobilized enzyme technology to co-immobilize trypsin and flavor protease on magnetic nanoparticles; S2. Preparation of active peptides: animal collagen raw materials and plant protein raw materials are subjected to ultrasonic-assisted defatting, crushed to 80-120 mesh, and immobilized trypsin and flavor protease are added for enzymatic hydrolysis; S3, Astragalus extract treatment, after crushing the Astragalus, reflux extraction with 60-80% ethanol 2-3 times, the extracts are combined, concentrated under reduced pressure until there is no alcohol taste, and spray dried to obtain powder; S4, probiotic treatment, centrifuging the probiotic fermentation broth to collect the bacteria, mixing it with the trehalose solution, and preparing the embedded bacterial powder by spray drying or freeze drying; S5. Formulation: Active peptides, vitamin-zinc complex, astragalus extract, probiotics and delivery carrier are mixed and made into enteric-soluble microspheres through fluidized bed coating.

7. The method for preparing the high-efficiency small molecule peptide preparation for restoring body functions according to claim 6, characterized in that: The preparation of the S2 active peptide specifically comprises the following steps: S201, defatting the animal collagen raw material and the plant protein raw material with the aid of ultrasonic wave, and crushing the raw material into 80-120 mesh; S202, immobilized trypsin and flavor protease were hydrolyzed at pH 7.5 and 50 °C for 5 h, and then immobilized on Fe3O4@SiO2-NH2 magnetic nanoparticles by glutaraldehyde cross-linking; S203, shaking the immobilized trypsin and flavor protease with the crushed protein raw material in an environment of pH 7.5-8.0 and temperature 50-55° C. for 2-6 hours; S204, collecting peptides with a molecular weight of 500-2000 Da through ultrafiltration membrane interception, separating the immobilized enzyme through a magnetic field strength of 0.3-0.5 T, washing the immobilized enzyme with a pH 7.0 buffer solution to remove the adsorbed peptides, and recovering the immobilized enzyme.

8. The method for preparing the high-efficiency small molecule peptide preparation for restoring body functions according to claim 6, characterized in that: The S5 preparation molding specifically includes the following steps: S501, dissolving the active peptide, vitamin-zinc complex, astragalus extract and acrylic resin in a mixed solvent of acetone and water; S502, using spray drying method to form pellets, with inlet temperature of 80-90°C and outlet temperature of 40-50°C; S503, the microspheres and the probiotic powder are mixed by a dry method.

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