Collagen dressing and preparation method thereof

Abstract

The invention discloses a collagen dressing and a preparation method thereof, and relates to the technical field of collagen dressings, and the collagen dressing comprises a dressing loaded on a gel-non-woven fabric composite carrier. According to the collagen dressing and the preparation method thereof provided by the invention, I / III type collagen compounding and small molecule peptide synergistic infiltration promotion, theaflavin-alginate oligosaccharide-trehalose ternary active matter intelligent stability maintenance, nano-encapsulation targeted delivery and gel-non-woven fabric composite carrier dynamic attachment are performed; the wound healing is accelerated, the scar rate is reduced, the transdermal rate of active ingredients is multiplied, the dressing change is free of damage, and the limitation that a traditional dressing is slow in repairing, many in scar and high in adhesion is broken through.

Description

Technical Field

[0001] The present invention relates to the technical field of collagen dressings, in particular to a collagen dressing and a preparation method thereof. Background Art

[0002] Collagen, a bioactive material, is a key protein component of the body's connective tissue, playing a vital role in supporting and protecting the body. It is primarily found in the skin, bones, tendons, and soft tissues. Due to its excellent biocompatibility, collagen has found widespread use in the medical and cosmetic fields.

[0003] Many existing medical dressings contain collagen. These collagen-containing dressings are often used for wound repair or trauma repair. They are applied by soaking a base material, such as a membrane, in a dressing solution containing collagen. When ready to use, the base material is applied to the face or other wound site to promote skin repair.

[0004] However, because the collagen content in collagen dressings is within a specific range, the viscosity of the dressing fluid is low. When the membrane cloth substrate is soaked in dressing fluid and removed for use, it is prone to dripping. This not only wastes the dressing fluid, but also severely impairs the repair effect of the dressing when applied to the face or wound. Summary of the Invention

[0005] The purpose of the present invention is to provide a collagen dressing and a preparation method thereof to solve the above-mentioned deficiencies in the prior art.

[0006] In order to achieve the above-mentioned object, the present invention provides the following technical solutions: a collagen dressing, comprising a dressing loaded on a gel-nonwoven composite carrier;

[0007] The dressing includes (by weight): 0.05–0.1 g of recombinant humanized type III collagen, 0.1–0.3 g of type I animal-derived collagen, 0.2–0.5 g of small molecule collagen peptides, 0.01–0.05 g of theaflavin, 0.05–0.2 g of brown algae oligosaccharides, 0.15–1 g of D-(+)-trehalose dihydrate, 0.15–0.5 g of carbomer, 1–5 g of glycerol, 90–180 g of purified water, and 3–5 g of a wrapping agent.

[0008] Preferably, the encapsulating agent is prepared by mixing sorbitan palmitate and β-vegetable oil in a mass ratio of 8g:3-4g.

[0009] Preferably, the recombinant humanized type III collagen has a complete triple helical structure, and its cell adhesion is ≥85%.

[0010] Preferably, the small molecule collagen peptide is hydrolyzed collagen, and its transdermal absorption rate is ≥90%.

[0011] Preferably, the encapsulating agent coats a mixture of the theaflavins and the brown algae oligosaccharides to form a liposome structure with a particle size of 50-200 nm.

[0012] Preferably, the gel layer on the gel-non-woven fabric composite carrier has a thickness of 0.2-0.5 mm, and the non-woven fabric has a grammage of 30-50 g / m².

[0013] A method for preparing a collagen dressing, which is used to prepare the collagen dressing described in the above scheme, is characterized in that the preparation method comprises the following steps:

[0014] 1) Add the type I animal-derived collagen to purified water at 4-10° C. and swell for 2-4 hours, then add the recombinant type III humanized collagen and the small molecule collagen peptide, and stir to form a collagen mixture;

[0015] 2) homogenizing the theaflavins, the brown algae oligosaccharides and the encapsulating agent at 50-60° C. to form nanoliposomes;

[0016] 3) Add the carbomer to the purified water at 50-60° C. and stir evenly, then add the glycerol and the D-(+)-trehalose dihydrate and stir until dissolved to prepare a matrix;

[0017] 4) Add the nanoliposomes to the matrix and mix thoroughly, then cool to 25-30°C and add the collagen mixture to prepare a viscous mixture;

[0018] 5) Apply the viscous mixture on non-woven fabric, cover with gel layer, and sterilize by irradiation.

[0019] Preferably, the pH value of the viscous mixed solution is adjusted to 6.0-6.5, and the viscosity thereof is controlled to be 5000-8000 mPa·s.

[0020] In the above technical solution, the present invention provides a collagen dressing and a preparation method thereof, which synergizes through the type I / III collagen compound system and small molecule collagen peptides, wherein: type III collagen activates fibroblast proliferation, type I collagen constructs a structural scaffold, and the small peptide penetrates the skin directly to the dermis, shortening the re-epithelialization time of the wound.

[0021] Secondly, trehalose has a water-locking capacity of 12 times its own weight, and the water vapor permeability of the dressing is optimized to maintain an ideal moist environment. DETAILED DESCRIPTION

[0022] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the embodiments.

[0023] Example 1

[0024] A collagen dressing includes a dressing loaded on a gel-nonwoven composite carrier, wherein the gel layer on the gel-nonwoven composite carrier has a thickness of 0.2-0.5 mm and the nonwoven fabric has a grammage of 30-50 g / m².

[0025] The dressing includes (by weight): 0.05–0.1 g of recombinant humanized type III collagen, 0.1–0.3 g of animal-derived type I collagen, 0.2–0.5 g of small molecule collagen peptides, 0.01–0.05 g of theaflavin, 0.05–0.2 g of fucoidan, 0.15–1 g of D-(+)-trehalose dihydrate, 0.15–0.5 g of carbomer, 1–5 g of glycerol, 90–180 g of purified water, and 3–5 g of wrapping agent.

[0026] Furthermore, the encapsulating agent is prepared by mixing sorbitan palmitate and β-vegetable oil in a mass ratio of 8g:3-4g.

[0027] Among them, recombinant humanized type III collagen has a complete triple helix structure and its cell adhesion is ≥85%.

[0028] Furthermore, small molecule collagen peptides are hydrolyzed collagen, and their transdermal absorption rate is ≥90%.

[0029] Furthermore, the encapsulating agent coats the mixture of theaflavins and brown algal oligosaccharides to form a liposome structure with a particle size of 50–200 nm.

[0030] Example 2

[0031] Based on Example 1, the dressing provided in this example includes (by weight): 0.05g of recombinant humanized type III collagen, 0.1g of type I animal-derived collagen, 0.2g of small molecule collagen peptides, 0.01g of theaflavin, 0.05g of brown algae oligosaccharides, 0.15g of D-(+)-trehalose dihydrate, 0.15g of carbomer, 1g of glycerol, 90g of purified water and 3g of wrapping agent.

[0032] Example 3

[0033] Based on Example 1, the dressing provided in this example includes (by weight): 0.8 g of recombinant humanized type III collagen, 0.2 g of type I animal-derived collagen, 0.35 g of small molecule collagen peptides, 0.025 g of theaflavin, 0.08 g of brown algae oligosaccharides, 0.7 g of D-(+)-trehalose dihydrate, 0.025 g of carbomer, 2.5 g of glycerol, 120 g of purified water, and 4 g of wrapping agent.

[0034] Example 4

[0035] Based on Example 1, the dressing provided in this example includes (by weight): 0.1g of recombinant humanized type III collagen, 0.3g of type I animal-derived collagen, 0.5g of small molecule collagen peptides, 0.05g of theaflavin, 0.2g of brown algae oligosaccharides, 1g of D-(+)-trehalose dihydrate, 0.5g of carbomer, 5g of glycerol, 180g of purified water and 5g of wrapping agent.

[0036] Example 5

[0037] Based on Example 2, the encapsulating agent provided in this example is prepared by mixing sorbitan palmitate and β-vegetable oil in a mass ratio of 8g:3g.

[0038] Example 6

[0039] Based on Example 2, the encapsulating agent provided in this example is prepared by mixing sorbitan palmitate and β-vegetable oil in a mass ratio of 8g:4g.

[0040] Example 7

[0041] This embodiment aims to provide a method for preparing the collagen dressing provided in the above embodiment, which comprises the following steps:

[0042] 1) Add animal-derived type I collagen to purified water at 4°C and swell for 2 hours. Add recombinant humanized type III collagen and small molecule collagen peptides and stir to form a collagen mixture.

[0043] 2) Homogenize theaflavins, brown algae oligosaccharides and the encapsulating agent at 50°C to form nanoliposomes;

[0044] 3) Add carbomer to purified water at 50–60°C and stir evenly, then add glycerol and D-(+)-trehalose dihydrate and stir until dissolved to prepare the matrix;

[0045] 4) Add the nanoliposomes to the matrix and mix thoroughly. Then cool the mixture to 25–30°C and add the collagen mixture to prepare a viscous mixture.

[0046] 5) Apply the viscous mixture to non-woven fabric, cover with gel layer, and sterilize by irradiation.

[0047] Furthermore, the pH value of the viscous mixture was adjusted to 6.0–6.5, and its viscosity was controlled to 5000–8000 mPa·s.

[0048] Example 8

[0049] This embodiment aims to provide a method for preparing the collagen dressing provided in the above embodiment 2, which comprises the following steps:

[0050] 1) Add animal-derived type I collagen to purified water at 4°C and swell for 2–4 hours. Add recombinant humanized type III collagen and small molecule collagen peptides and stir to form a collagen mixture.

[0051] 2) Homogenize theaflavins, brown algae oligosaccharides and the encapsulating agent at 50°C to form nanoliposomes;

[0052] 3) Add carbomer to purified water at 50°C and stir evenly, then add glycerol and D-(+)-trehalose dihydrate and stir until dissolved to prepare the matrix;

[0053] 4) Add the nanoliposomes to the matrix and mix thoroughly, then cool to 25°C and add the collagen mixture to prepare a viscous mixture;

[0054] 5) Apply the viscous mixture to non-woven fabric, cover with gel layer, and sterilize by irradiation.

[0055] Furthermore, the pH value of the viscous mixture was adjusted to 6.0–6.5, and its viscosity was controlled to 5000–8000 mPa·s.

[0056] Embodiment 9

[0057] This embodiment aims to provide a method for preparing the collagen dressing provided in the above embodiment 2, which comprises the following steps:

[0058] 1) Add animal-derived type I collagen to purified water at 10°C and swell for 2–4 hours. Add recombinant humanized type III collagen and small molecule collagen peptides and stir to form a collagen mixture.

[0059] 2) Homogenize theaflavins, brown algae oligosaccharides and the encapsulating agent at 60°C to form nanoliposomes;

[0060] 3) Add carbomer to purified water at 60°C and stir evenly, then add glycerol and D-(+)-trehalose dihydrate and stir until dissolved to prepare the matrix;

[0061] 4) Add the nanoliposomes to the matrix and mix thoroughly. Then cool the mixture to 25–30°C and add the collagen mixture to prepare a viscous mixture.

[0062] 5) Apply the viscous mixture to non-woven fabric, cover with gel layer, and sterilize by irradiation.

[0063] Furthermore, the pH value of the viscous mixture was adjusted to 6.0–6.5, and its viscosity was controlled to 5000–8000 mPa·s.

[0064] Example 10

[0065] The dressings prepared according to the dressing ratios provided in Examples 2, 3, and 4 were prepared using Example 8. These dressings were then divided into three experimental groups for testing, with each group consisting of 10 rats. The burn wounds on the exposed skin of the rats were treated identically to achieve the same burn grade. Continuous application of the dressings was then performed to collect data on healing time, collagen activity, and transdermal absorption rate after healing, resulting in the following table (see Table 1):

[0066] Project days Healing time Collagen activity protection Transdermal absorption rate Example 2 8.2 days Greater than 92% 85%～87% Example 3 7.2 days More than 95% 90%～92% Example 4 7.2 days Greater than 95.5% 91%～92.3%

[0067] In summary, combined with the above comparative data, it can be seen that when the dressing configuration changes from Example 3 to Example 4, the data difference is not large, but when comparing Example 2 with Example 3, the data difference is obvious. It is obvious that the best configuration of the dressing is the configuration ratio provided by Example 3, but the profitability does not increase significantly with the increase of the ratio.

[0068] Example 11

[0069] The dressings prepared according to the dressing ratios provided in Examples 5 and 6 were prepared in Example 8. The resulting dressings were then divided into two experimental groups for testing, with each group consisting of 10 rats. The burn wounds on the exposed skin of the rats were treated identically to achieve the same burn grade. Continuous application of the dressings was then performed to collect data on healing time, collagen activity, and transdermal absorption rate after healing, resulting in the following table (see Table 1):

[0070] Project days Healing time Collagen activity protection Transdermal absorption rate Example 5 7.3 days Greater than 91% 85%～87% Example 6 7.2 days More than 90% 86%～87%

[0071] In summary, combined with the above comparative data, it can be seen that modifying the ratio of sorbitan palmitate and β-vegetable oil in the wrapping agent has no significant effect on the therapeutic effect of the dressing.

[0072] Example 12

[0073] The dressing provided in Example 2 was prepared according to the preparation processes provided in Examples 8 and 9. The resulting dressings were then divided into two experimental groups for testing, with each group consisting of 10 rats. The burn wounds on the exposed skin of the rats were treated identically to achieve the same burn grade. Data on healing time, collagen activity after healing, and transdermal absorption rate were collected through continuous application, resulting in the following table (see Table 1):

[0074] Project days Healing time Collagen activity protection Transdermal absorption rate Example 8 8.2 days Greater than 92% 85%～87% Embodiment 9 8.2 days Greater than 92% 85%～87%

[0075] In summary, combined with the above comparative data, it can be seen that changes in temperature during the process will not increase the dressing effect.

[0076] In summary, the present invention provides a synergistic effect through the combination of type I / III collagen and small-molecule collagen peptides: type III collagen activates fibroblast proliferation, type I collagen builds a structural scaffold, and the small peptides penetrate directly into the dermis, shortening the time it takes for wounds to re-epithelialize. Furthermore, trehalose's ability to lock in 12 times its own weight in moisture optimizes the water vapor permeability of the dressing, maintaining an ideal moist environment.

[0077] The above descriptions of certain exemplary embodiments of the present invention are provided by way of illustration only. It is understood that those skilled in the art will be able to modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above embodiments and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims.

Claims

1. A collagen dressing, characterized in that: The invention comprises a dressing, which is loaded on a gel-nonwoven composite carrier; The dressing includes (by weight): 0.05–0.1 g of recombinant humanized type III collagen, 0.1–0.3 g of type I animal-derived collagen, 0.2–0.5 g of small molecule collagen peptides, 0.01–0.05 g of theaflavin, 0.05–0.2 g of brown algae oligosaccharides, 0.15–1 g of D-(+)-trehalose dihydrate, 0.15–0.5 g of carbomer, 1–5 g of glycerol, 90–180 g of purified water, and 3–5 g of a wrapping agent.

2. A collagen dressing according to claim 1, characterized in that: The encapsulating agent is prepared by mixing sorbitan palmitate and beta-vegetable oil in a mass ratio of 8g:3-4g.

3. A collagen dressing and a preparation method thereof according to claim 1, characterized in that: The recombinant humanized type III collagen has a complete triple helical structure, and its cell adhesion is ≥85%.

4. The collagen dressing according to claim 1, characterized in that: The small molecule collagen peptide is hydrolyzed collagen, and its transdermal absorption rate is ≥90%.

5. The collagen dressing according to claim 1, characterized in that: The encapsulating agent coats a mixture of the theaflavins and the brown algae oligosaccharide to form a liposome structure with a particle size of 50-200 nm.

6. The collagen dressing according to claim 1, characterized in that: The gel layer on the gel-non-woven fabric composite carrier has a thickness of 0.2-0.5 mm, and the non-woven fabric has a grammage of 30-50 g / m².

7. A method for preparing a collagen dressing, which is used to prepare the collagen dressing according to any one of claims 1 to 6, characterized in that: The preparation method comprises the following steps: 1) Add the type I animal-derived collagen to purified water at 4-10° C. and swell for 2-4 hours, then add the recombinant type III humanized collagen and the small molecule collagen peptide, and stir to form a collagen mixture; 2) homogenizing the theaflavins, the brown algae oligosaccharides and the encapsulating agent at 50-60° C. to form nanoliposomes; 3) Add the carbomer to the purified water at 50-60° C. and stir evenly, then add the glycerol and the D-(+)-trehalose dihydrate and stir until dissolved to prepare a matrix; 4) Add the nanoliposomes to the matrix and mix thoroughly, then cool to 25-30°C and add the collagen mixture to prepare a viscous mixture; 5) Apply the viscous mixture on non-woven fabric, cover with gel layer, and sterilize by irradiation.

8. A collagen dressing and a preparation method thereof according to claim 7, characterized in that: The pH value of the viscous mixed solution is adjusted to 6.0-6.5, and the viscosity thereof is controlled to be 5000-8000 mPa·s.