Solid beverage for maintaining intestinal axis and brain axis as well as preparation method and application of solid beverage

Abstract

The invention relates to a solid beverage for maintaining intestinal-brain axes as well as a preparation method and application of the solid beverage, and belongs to the technical field of food processing. The health-care tea powder consists of cold-extracted freeze-dried tea powder, theanine powder, fructus phyllanthi powder, PQQ powder or microencapsulated PQQ powder, targeted probiotic microcapsule powder, mogroside and galactooligosaccharide. During preparation, the cold-extracted freeze-dried tea powder, the microencapsulated PQQ powder and the purchased targeted probiotic microcapsule powder are respectively prepared and then are mixed with other raw materials. The innovation of the invention lies in that the intestine-brain axis is synergistically maintained through a four-dimensional mechanism of anti-inflammatory and anti-oxidation-neuromodulation-mitochondrial activation-flora intervention, and the activity of the components is guaranteed by adopting technologies of cold extraction freeze-drying, microencapsulation and the like. The system can inhibit enteropathogenic bacteria, repair intestinal barriers, protect nervous systems, improve cognitive functions and regulate intestinal flora, has high technical feasibility and market prospects, and makes remarkable progress compared with the prior art.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of food processing, and particularly relates to a solid beverage for maintaining the gut-brain axis and a preparation method and application thereof. BACKGROUND

[0002] The gut-brain axis refers to a bidirectional communication system between the gut and the brain, which realizes information transmission between the gut and the brain through nerve, immune, metabolic and other pathways. Studies have shown that the gut health is closely related to the brain function, and intestinal problems such as intestinal flora imbalance and impaired intestinal barrier function may lead to abnormal brain function, such as anxiety, depression, cognitive impairment, etc.; conversely, the state of the brain such as mood and stress may also affect the function of the gut, such as leading to irritable bowel syndrome. Therefore, regulating the function of the gut-brain axis is of great significance for maintaining human health.

[0003] At present, there are few functional foods on the market for maintaining the function of the gut-brain axis, and most of them have problems such as single ingredient, few target points, and insignificant effect. Therefore, it is of great market prospect and social significance to develop a functional food that can synergistically maintain the function of the gut-brain axis through multiple target points.

[0004] In view of this, the present application is proposed to solve the problems of the prior art. SUMMARY

[0005] The purpose of the present application is to provide a solid beverage for maintaining the gut-brain axis and a preparation method and application thereof.

[0006] To achieve the above-mentioned purpose, the present application provides the following technical solution: A solid beverage for maintaining the gut-brain axis, comprising the following raw materials by weight: 10-60 parts of cold-brewed freeze-dried tea powder, 1-20 parts of tea theanine powder, 3-15 parts of phyllanthus emblica powder, 0.3-2 parts of microencapsulated PQQ powder, 1-10 parts of targeted probiotic microcapsule powder, 0.5 parts of mogroside and 0.05-0.1 parts of galacto-oligosaccharide.

[0007] Further, the microencapsulated PQQ powder is replaced by PQQ powder, and the weight fraction of the PQQ powder is 0.1-0.7 parts.

[0008] A preparation method of the above-mentioned solid beverage for maintaining the gut-brain axis, comprising the following steps: S1: preparation of cold-brewed freeze-dried tea powder The tea leaves and water are cold-brewed by the immersion method at a ratio of 1:40-60, and the water temperature is 2-6℃; The obtained cold extraction solution is placed into a refrigerating machine for vacuum freeze drying, pre-freezing temperature is -40 DEG C, then sublimation is carried out, sublimation parameters are -25 DEG C / 50 Pa, then powder treatment is carried out, and cold extraction freeze-dried tea powder is obtained; S2: Preparation of theanine powder The theanine crude product is obtained by water extraction, filtration and concentration through tea leaf extraction method, and is further purified through membrane separation technology, and finally high-purity theanine powder is obtained. S3: Preparation of microencapsulated PQQ The purchased PQQ powder is microencapsulated by spray drying, the inlet temperature is 180 DEG C, and the ratio of core material to wall material is 1:3. S4: Solid beverage mixing The cold extraction freeze-dried tea powder and the theanine powder are mixed in a mixer for 10 minutes at a rotating speed of 20 rpm, and then the microencapsulated PQQ powder, the purchased targeted probiotic microcapsule powder, the phyllanthus emblica powder, the mogroside and the galactooligosaccharide are added and mixed for 15 minutes to ensure that the RSD is less than 5%, and finally the product is obtained.

[0009] The application of the above-mentioned solid beverage for the maintenance of the gut-brain axis includes serving as a tea beverage base or a single packaged powder solid beverage.

[0010] The beneficial effects of the present application are as follows: 1. Multi-dimensional targeted gut-brain axis mechanism innovation: The present application combines tea polyphenols (cold extraction tea powder), theanine, PQQ and targeted probiotics to form a four-dimensional linkage mechanism of "anti-inflammatory and antioxidant-neural regulation-mitochondrial activation-bacterial intervention", which breaks through the traditional path of single intestinal or brain function regulation. Among them, the phyllanthus emblica (emblica officinalis) contains rich hydrolyzed tannin (≥15%), and the gallic acid thereof induces IL-10 secretion through intestinal epithelial cells to inhibit the NF-κB inflammatory pathway; the targeted probiotics (such as specific fermented lactobacillus mucus / lactobacillus plantarum / lactobacillus reuteri) up-regulate brain-derived neurotrophic factor (BDNF), achieving bidirectional regulation of intestinal barrier repair and neural inflammation inhibition.

[0011] 2. Cross-border fusion innovation of ingredients and process: Cold extraction freeze-dried tea powder activity retention: The low-temperature extraction process improves the tea polyphenol retention rate to more than 85% (only 60% for traditional hot air drying), while reducing the precipitation of caffeine and avoiding the risk of intestinal irritation.

[0012] Microencapsulation complex embedding technology: PQQ is embedded with β-cyclodextrin-sodium alginate complex wall material to improve high-temperature stability (resistant to 60 DEG C storage, degradation rate <5% per month); targeted probiotics are embedded with double-layer microcapsules (inner layer of polylactic acid-glycolic acid copolymer + outer layer of pectin) to achieve a survival rate of >90% in gastric acid environment and colon-targeted release.

[0013] 3. Differentiated raw material combination synergies: Synergy between Yunnan olives and theanine: Gallic acid (GA) in Yunnan olives and theanine inhibit monoamine oxidase B (MAO-B), synergistically increase 5-hydroxytryptamine (5-HT) levels, and improve anxiety and irritable bowel syndrome-related symptoms.

[0014] Functional coupling of PQQ and probiotics: PQQ promotes intestinal mitochondrial biogenesis by activating the PGC-1α pathway, and the combination of PQQ and probiotics (such as Lactobacillus plantarum H6) can increase butyric acid production by 38% and enhance the efficiency of gut-brain axis signal transmission.

[0015] 4. High technical feasibility: Formulation ratio adaptability: Cold-brewed tea powder accounts for 10-60% of the total, covering different functional needs of green tea, white tea, black tea, and Pu'er tea; targeted probiotics (1-10%) can be adjusted according to the characteristics of the strain.

[0016] Process compatibility: existing freeze-drying and microencapsulation embedding processes are mature, and the double-helix blade counter-rotation mixing system can solve the problem of homogenization of multi-phase materials.

[0017] 5. Significant functional effect: In vitro simulation experiments show that the combination of cold-brewed tea powder (Pu'er ripe tea) and Yunnan olive powder can inhibit intestinal pathogenic bacteria (such as Escherichia coli) by 27%.

[0018] Animal experiments show that the combination of PQQ (0.5% addition) and probiotics can improve the correct rate of Y maze in experimental mice by 19% (p<0.05).

[0019] 6. Wide market prospects: Targeting the "brain fatigue + irritable bowel" sub-health population (accounting for 42% of urban white-collar workers), it precisely targets the office and overtime scenarios.

[0020] The market for probiotic solid beverages is growing at a rate of 25% per year, and the premium space for brain health concepts is significant.

[0021] 7. Strong regulatory compliance: Theanine, PQQ (≤20mg / d), and specific probiotics (such as Lactobacillus) have been included in the list of new food raw materials and edible strains in China; Yunnan olives can be used as ordinary food raw materials according to the Chinese Pharmacopoeia.

[0022] The above description is only a summary of the technical solutions of the present application. In order to more clearly understand the technical means of the present application, and to implement the content of the description, the following will be described in detail with the preferred embodiments of the present application. DETAILED DESCRIPTION

[0023] The technical solutions of the present application will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0024] In addition, the technical features involved in different embodiments of the present application described below can be combined with each other as long as they do not conflict with each other. Embodiment 1

[0025] The solid beverage for maintaining the gut-brain axis shown in a preferred embodiment of the present application comprises the following raw materials by weight: 10 parts of cold-brewed freeze-dried tea powder, 5 parts of theanine powder with a purity of 20%, 3 parts of phyllanthus emblica (dian olive) micro powder, 0.3 parts of microencapsulated PQQ powder, 1 part of targeted probiotic microcapsule powder, 0.5 parts of mogroside, and 0.05 parts of galactooligosaccharide.

[0026] Among them, PQQ is short for Pyrroloquinoline Quinone, the Chinese name is pyrroloquinoline quinone, and the CAS registration number is 72909-34-3. It is a quinone compound with redox activity, widely exists in microorganisms, plants and animals. It participates in intracellular oxidation-reduction reaction as a coenzyme, and may affect the health of organisms by supporting mitochondrial function, antioxidant and regulating gene expression, etc.

[0027] It should be noted that the PQQ powder and the targeted probiotic microcapsule powder in the present application are obtained by purchase.

[0028] In addition, the above-mentioned targeted probiotics include fermented Lactobacillus mucus / Lactobacillus reuteri, Lactobacillus plantarum, can secrete mucin binding proteins (MUBs), enhance the thickness of the intestinal mucus layer, block pathogens, and up-regulate the expression of occludin (ZO-1) and claudin, repair the tight junction of intestinal epithelium. Embodiment 2

[0029] The solid beverage for maintaining the gut-brain axis shown in a preferred embodiment of the present application comprises the following raw materials by weight: 60 parts of cold-brewed freeze-dried tea powder, 10 parts of theanine powder with a purity of 40%, 15 parts of phyllanthus emblica micro powder, 2 parts of microencapsulated PQQ powder, 10 parts of targeted probiotic microcapsule powder, 0.5 parts of mogroside, and 0.1 parts of galactooligosaccharide.

[0030] Specifically, the preparation of the above-mentioned solid beverage comprises the following steps: (I) Preparation of cold-brewed freeze-dried tea powder The tea leaves and water are cold-brewed by the immersion method at a ratio of 1:50, and the water temperature is 4℃. The obtained cold-brewed liquid is put into a refrigerating machine for vacuum freeze-drying, the pre-freezing temperature is -40℃, and then the sublimation stage parameters are -25℃ / 50Pa to sublimate and dry the solid water in the freeze-drying; The obtained freeze-dried powder is ground to obtain the cold-brewed freeze-dried tea powder with a moisture content of ≤5%.

[0031] (II) Preparation of theanine powder The theanine crude product is obtained by water extraction, filtration and concentration through tea leaf extraction, and then purified by membrane separation technology, and finally the high-purity theanine powder is obtained.

[0032] (III) Preparation of microencapsulated PQQ powder The purchased PQQ powder is microencapsulated by spray drying (inlet temperature 180℃, core material / wall material ratio 1:3) to obtain the microencapsulated PQQ powder.

[0033] (IV) Solid beverage mixing The cold-brewed freeze-dried tea powder and theanine powder are mixed in a V-type mixer for 10 minutes at a speed of 20 rpm, and then the microencapsulated PQQ powder, targeted probiotic microcapsule powder, phyllium powder and other additives are added and mixed for 15 minutes to ensure that the RSD is ≤5%, and finally the product is obtained.

[0034] It should be noted that in other embodiments, PQQ powder can be used directly instead of microencapsulated PQQ, and the weight of PQQ powder is 1 / 3 of that of microencapsulated PQQ, i.e. 0.7 parts.

[0035] Specifically, the application of the present application includes the preparation of various flavored beverages with other beverages including but not limited to the present tea-based solid beverage and fresh vegetable and fruit juice, yogurt, milk and other liquid beverages.

[0036] Further, the present application can also be used as a single packaged powder solid beverage. Example 3

[0037] I. In vitro experiment Inhibition of intestinal pathogenic bacteria: the solid beverage prepared in Example 1 is dissolved in physiological saline at a concentration of 1%, 100 μL is added to the culture medium containing E. coli, and after 24 hours of incubation at 37℃, the number of E. coli is determined. The results show that the inhibition rate of the complex system on E. coli is 27%, while the inhibition rate of cold-brewed freeze-dried green tea powder alone is 15%, and the inhibition rate of phyllium powder alone is 10%, indicating that the complex system has a synergistic effect on inhibiting intestinal pathogenic bacteria.

[0038] Effect on intestinal barrier function: The complex system prepared in Example 2 was added to the cell culture medium at a concentration of 0.1 mg / mL using the Caco-2 / HT29-MTX intestinal barrier model. After 48 hours of culture, the expression level of tight junction protein occludin was measured. The results showed that the complex system significantly increased the expression level of occludin (p<0.05), indicating that the complex system had the effect of repairing intestinal barrier function.

[0039] II. Animal experiments 1. Effect on cognitive function: 60 healthy male mice were selected and randomly divided into 6 groups, 10 mice in each group. They were respectively the control group, the model group, the theanine group (200 mg / kg), the PQQ group (10 mg / kg), the probiotic group (1 x 10^9 CFU / kg), and the complex system group (converted dose according to the formula of Example 2). Except for the control group, the rest of the mice were constructed into anxiety models by chronic stress method. After the modeling was successful, the mice in each group were given corresponding drugs or normal saline by gavage once a day for 28 consecutive days. After the last administration, the cognitive function of the mice was determined by Y maze experiment. The results showed that compared with the model group, the Y maze correct rate of the mice in the complex system group increased by 19% (p<0.05), which was significantly higher than that in the theanine group, the PQQ group and the probiotic group (p<0.05), indicating that the complex system had the effect of synergistically improving cognitive function.

[0040] 2. Effect on intestinal flora: 60 healthy male rats were selected and randomly divided into 6 groups, 10 rats in each group. They were respectively the control group, the model group, the low-dose complex system group (1 / 2 of the dose according to the formula of Example 2), the medium-dose complex system group (the dose according to the formula of Example 2), the high-dose complex system group (2 times the dose according to the formula of Example 2), and the positive control group (given a certain commercially available probiotic solid beverage). Except for the control group, the rest of the rats were constructed into intestinal flora imbalance models by high-fat diet method. After the modeling was successful, the rats in each group were given corresponding drugs or normal saline by gavage once a day for 28 consecutive days. After the last administration, the feces of the rats were collected, and the composition of intestinal flora was analyzed by 16S rRNA sequencing technology. The results showed that compared with the model group, the number of beneficial bacteria such as Bifidobacterium and Lactobacillus in the intestines of rats in the medium-dose complex system group increased significantly (p<0.05), and the number of harmful bacteria such as Escherichia coli and Enterococcus decreased significantly (p<0.05), and the effect was better than that of the positive control group (p<0.05), indicating that the complex system had the effect of regulating the balance of intestinal flora.

[0041] The technical features of the above-described embodiments can be combined in any manner. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described, however, as long as the combinations of the technical features do not contradict each other, they should be considered within the scope of the present disclosure.

[0042] The above embodiments only express several implementation manners of the present application, which are described in a more specific and detailed manner, but cannot be understood as a limitation on the patent scope of the present application. It should be noted that, for ordinary skilled persons in the art, several modifications and improvements can be made without departing from the concept of the present application, which all belong to the protection scope of the present application. Therefore, the protection scope of the patent of the present application should be subject to the appended claims.

Claims

1. A solid beverage for maintaining the gut-brain axis, characterized in that: The raw materials include the following parts by weight: 10-60 parts of cold-brewed freeze-dried tea powder, 1-20 parts of tea theanine powder, 3-15 parts of emblica powder, 0.3-2 parts of microencapsulated PQQ powder, 1-10 parts of targeted probiotic microcapsule powder, 0.5 parts of mogroside and 0.05-0.1 parts of galacto-oligosaccharide.

2. The solid beverage for maintaining the gut-brain axis according to claim 1, characterized in that: The microencapsulated PQQ powder is replaced with PQQ powder, and the weight portion of the PQQ powder is 0.1-0.7 parts.

3. A method for preparing a solid beverage for maintaining the gut-brain axis according to claim 1, characterized in that: The following steps are involved: S1: Preparation of cold-extracted freeze-dried tea powder Cold brew tea leaves and water at a ratio of 1:40-60 using the maceration method, with the water temperature at 2-6°C. The obtained cold extract is placed in a freezer for vacuum freeze drying at a pre-freezing temperature of -40°C, followed by sublimation at a sublimation parameter of -25°C / 50Pa, and then ground to obtain cold-extracted freeze-dried tea powder; S2: Preparation of theanine powder Theanine crude product is obtained through water extraction, filtration and concentration by tea extraction method, and then further purified by membrane separation technology to finally obtain high-purity theanine powder; S3: Preparation of Microencapsulated PQQ The purchased PQQ powder was microencapsulated by spray drying, with an inlet air temperature of 180°C and a core material to wall material ratio of 1:3; S4: Solid drink mix The above-mentioned cold-extracted freeze-dried tea powder and theanine powder were added to a mixer and mixed for 10 minutes at a speed of 20 rpm. Then, microencapsulated PQQ powder, purchased targeted probiotic microcapsule powder, emblica micropowder, mogroside, and galacto-oligosaccharide were added and mixed for 15 minutes to ensure that RSD was ≤5%. Finally, this product was obtained.

4. A use of a solid beverage for maintaining the gut-brain axis according to claim 1, characterized in that: Including as a tea beverage base, or as an individually packaged powdered solid beverage.