Integrated device for automatic enrichment and physiological phenotype and species identification of zooplankton

Abstract

The invention relates to a zooplankton automatic enrichment and physiological phenotype and species identification integrated device, which comprises a zooplankton enrichment module, a physiological phenotype data acquisition module, a species data acquisition module and a data processing identification module, the physiological phenotype data acquisition module comprises an automatic sorting mechanism and a plurality of observation bins, the temporary storage bin is communicated with the observation bins through the automatic sorting mechanism, the species data acquisition module comprises a treatment liquid adding mechanism, a plurality of treatment bins, a sampling pump and a second collector, and the observation bins are communicated with the treatment bins in a one-to-one correspondence mode through pipelines provided with liquid supply pumps. The first collector and the second collector are both in signal connection with the data processing identification module. The method has the advantages that the efficiency of collecting and analyzing the basic information of the zooplankton in the water body is improved, and the pollution state and the future evolution pattern of the water body can be comprehensively predicted through the two-dimensional information of the physiological state and the variety diversity.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of aquatic ecological environment and plankton monitoring, and particularly relates to a device for automatic enrichment, physiological phenotype and species identification of plankton. BACKGROUND

[0002] Plankton is an important component of the water ecological evaluation index system. Current plankton monitoring mainly relies on manual microscopic examination or automatic image acquisition combined with manual identification, which is time-consuming and labor-intensive, greatly limiting the time resolution of water ecological evaluation as an index. There are some technologies that attempt to use automatic equipment to collect images of fixed water samples and then identify plankton species through AI recognition. However, these technologies still require manual enrichment of plankton to prepare monitoring samples, which is also time-consuming and has errors caused by human factors. Therefore, neither traditional techniques nor newly emerging AI intelligent identification can effectively solve the problems of time consumption, labor intensity, and errors caused by human factors.

[0003] In addition, only obtaining information on the diversity of plankton and lacking information on the physiological phenotype of plankton cannot predict and evaluate the impact of sudden environmental pollution and extreme climate change on specific biological groups, and thus cannot guide the development of ecological risk prevention and control plans for pollution events. This is determined by the nonlinearity, hysteresis, or lack of elasticity of biological diversity in response to environmental stress. In other words, neither traditional manual identification nor recent machine identification can cover the health status information of plankton from individual to population in a specific water area, i.e., diversity information cannot take into account physiological state information, and thus cannot provide prediction information on the pollution status of the water area and the changing trend of the plankton community. The physiological phenotype and diversity information of plankton have equal important information value for ecological environment monitoring, but there is currently no related technology reported that can simultaneously collect these two-dimensional information. SUMMARY

[0004] The technical problem to be solved by the present application is to provide a device for automatic enrichment, physiological phenotype and species identification of plankton, which overcomes the above-mentioned deficiencies in the prior art.

[0005] The technical scheme solving the above technical problems of the present application is as follows: An automatic zooplankton enrichment, physiological phenotype and species identification integrated device, which comprises a zooplankton enrichment module, a physiological phenotype data acquisition module, a species data acquisition module and a data processing and identification module, the zooplankton enrichment module comprises a non-destructive dehydration and concentration mechanism and a temporary storage bin, the physiological phenotype data acquisition module comprises an automatic sorting mechanism and a plurality of observation bins, the temporary storage bin is communicated with each observation bin through the automatic sorting mechanism, a plurality of phenotype identification windows are arranged on each observation bin, a first collector is arranged at the phenotype identification window, the species data acquisition module comprises a treatment liquid adding mechanism, a plurality of treatment bins, a sampling pump and a second collector, the treatment liquid adding mechanism is used for adding treatment liquid into the treatment bin, the treatment bin supplies the detection end of the second collector with a sample to be tested through a pipeline provided with the sampling pump, the temporary storage bin is communicated with the concentration liquid discharge port of the non-destructive dehydration and concentration mechanism through a pipeline, and the observation bin is communicated with the treatment bin one by one through a pipeline provided with a liquid supply pump, and the first collector and the second collector are both signal connected with the data processing and identification module.

[0006] On the basis of the above technical scheme, the present application can also be improved as follows.

[0007] Further, the non-destructive dehydration and concentration mechanism comprises an outer shell with an open upper end, a water containing barrel and a dehydration barrel, the outer shell is provided with an openable cover at the upper end, the water containing barrel is fixedly arranged in the outer shell, the dehydration barrel is located in the water containing barrel, a filter membrane is arranged on the inner wall of the dehydration barrel, the center of the bottom of the dehydration barrel is transmissionally connected with a driving assembly through a rotating shaft penetrating the bottom wall of the water containing barrel, a flow guide channel is formed in the center of the rotating shaft, the lower end of the rotating shaft is communicated with a concentration liquid discharge pipe through a rotating sealing element, the concentration liquid discharge pipe is communicated with the inlet of an external pump, the outlet of the external pump is the concentration liquid discharge port, the concentration liquid discharge port is communicated with the temporary storage bin through a pipeline, a water inlet mechanism is arranged above the dehydration barrel, a drainage mechanism is arranged at the bottom of the water containing barrel, and a backwashing nozzle and a backblowing nozzle are arranged between the water containing barrel and the dehydration barrel.

[0008] Further, the backwashing nozzle is communicated with the outlet of a backwashing pump through a backwashing pipeline, the inlet of the backwashing pump is communicated with a clean water storage tank through a pipeline, the backblowing nozzle is communicated with an air compressor or a compressed gas storage tank through a backblowing pipeline, and electric valves are respectively arranged on the backwashing pipeline and the backblowing pipeline.

[0009] Further, a balance ring assembly is arranged between the top of the dehydration barrel and the top of the water containing barrel.

[0010] Further, a positioning sleeve is detachably connected in the dehydration barrel, the sleeve wall of the positioning sleeve is designed to be hollow and the filter membrane is tightly positioned on the inner wall of the dehydration barrel.

[0011] Further, a liquid level sensor is arranged on the inner wall of the dehydration barrel, the distance between the liquid level sensor and the bottom wall of the dehydration barrel is 5-8 cm, the space below the liquid level sensor in the dehydration barrel is a concentrated liquid collecting cavity, and the sidewall of the dehydration barrel is densely provided with dehydration holes in the area above the concentrated liquid collecting cavity.

[0012] Further, the automatic sorting mechanism comprises a cylinder body provided with end covers at two ends, and a plurality of levels of zooplankton collecting nets with mesh sizes decreasing in sequence are arranged in the cylinder body from the inlet end to the outlet end in sequence and at intervals, and the fine end of each zooplankton collecting net is provided with an electromagnetic valve.

[0013] Further, the first collector comprises a camera and / or a Doppler ultrasound monitor, and the second collector comprises a camera, a camera or a biological microscope.

[0014] Further, the treatment liquid adding mechanism comprises a liquid adding funnel, and the bottom of the liquid adding funnel is provided with an electromagnetic valve and a flow meter.

[0015] Further, the treatment liquid adding mechanism further comprises a fixing frame, a sliding block and a sliding rail, the treatment bins are arranged in sequence and adjacent to each other along a straight line, one sliding rail is fixed to the same side of each treatment bin, one end of the fixing frame is fixedly connected with the sliding block, and the other end is fixedly connected with the liquid adding funnel, and when the sliding block slides along the sliding rail, the liquid adding funnel can pass above each treatment bin in sequence.

[0016] Compared with the prior art, the beneficial effects of the present application are:

[0017] The present application integrates zooplankton enrichment, physiological phenotype collection and intelligent species identification, can quickly and automatically obtain the physiological state and species information of the target water body, avoids manual sampling and detection in the whole process, and is comprehensive, accurate and efficient in information collection, saves time and labor, and avoids human error. The integrated device provided by the present application can collect information of zooplankton comprehensively, can cover the health state information of zooplankton from individual to population in a specific water area, and can consider physiological state information and diversity information, and at the same time, through the addition of treatment liquid (such as toxic metals, insecticides and other characteristic pollutants), a zooplankton community poisoning response test is carried out, the influence of a chemical leakage event on the zooplankton community in the vicinity of the water body is simulated, and then a response plan is proposed. The present application improves the efficiency of analyzing the state of zooplankton in the water body, and through the two-dimensional information of physiological state and species diversity, the pollution state and future evolution pattern of the water body can be comprehensively predicted.

[0018] The present application can sample and enrich the target water body containing zooplankton without damage, adopt low-speed rotation dehydration, and blow air flow from the outside of the filter membrane intermittently through the back-blowing nozzle during the period. The air flow back-blowing makes the zooplankton adhering to the filter membrane separate, and water is more likely to penetrate into the water bucket through the filter membrane. The zooplankton separates from the filter membrane in time under the action of the air flow, avoids damage to the zooplankton caused by long-term accumulation and adhesion on the filter membrane, and at the same time, the concentrated zooplankton can be washed off after concentration to avoid quantity loss. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 A structural block diagram of an automatic zooplankton enrichment, physiological phenotype and species intelligent identification integrated device for water body provided by the present application is shown in the figure.

[0020] Figure 2 For Figure 1 A structural diagram of a lossless dehydration and concentration mechanism of a zooplankton enrichment module in the device is shown in the figure.

[0021] Figure 3 For Figure 1 A connection relationship diagram of a zooplankton enrichment module, a physiological phenotype data acquisition module and a species data acquisition module in the device is shown in the figure.

[0022] Figure 4 For Figure 3 A positional relationship diagram of a certain observation bin and a first collector in the device is shown in the figure.

[0023] Figure 5 For Figure 3 A connection relationship diagram of a certain treatment bin, a second collector and a liquid adding funnel in the device is shown in the figure.

[0024] In the drawings, the components represented by each reference numeral are listed as follows:

[0025] 100, zooplankton enrichment module; 200, physiological phenotype data acquisition module; 300, species data acquisition module; 400, data processing and identification module;

[0026] 101, lossless dehydration and concentration mechanism; 102, temporary storage bin; 103, shell; 104, water bucket; 105, dehydration bucket; 106, cover; 107, rotating shaft; 108, driving assembly; 109, external discharge pump; 110, water inlet mechanism; 111, drainage mechanism; 112, backwashing nozzle; 113, back-blowing nozzle; 114, backwashing pump; 115, air compressor; 116, balance ring assembly; 117, positioning sleeve; 118, liquid level sensor;

[0027] 201, automatic sorting mechanism; 202, observation bin; 203, zooplankton collection net; 204, electromagnetic valve; 205, first collector;

[0028] 301, treatment bin; 302, sampling pump; 303, second collector; 304, liquid adding funnel; 305, fixing frame; 306, sliding block; 307, sliding rail. DETAILED DESCRIPTION

[0029] The principles and features of the present application are described below in conjunction with the accompanying drawings and specific embodiments, which are only used to explain the present application and are not intended to limit the scope of the present application.

[0030] In the description of the present application, if the terms indicating the orientation or position relationship such as "up", "down", "left", "right", "top", "bottom", "inner", "outer" are used, the indicated orientation or position relationship is based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the present application and simplifying the description, and is not intended to indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0031] As shown in Figures 1 to 5 The present application provides an automatic zooplankton enrichment, physiological phenotype and species identification integrated device, which comprises a zooplankton enrichment module 100, a physiological phenotype data acquisition module 200, a species data acquisition module 300 and a data processing and identification module 400. The zooplankton enrichment module 100 comprises a non-destructive dehydration and concentration mechanism 101 and a temporary storage bin 102. The physiological phenotype data acquisition module 200 comprises an automatic sorting mechanism 201 and a plurality of observation bins 202. The temporary storage bin 102 is in communication with each observation bin 202 through the automatic sorting mechanism 201. Each observation bin 202 is provided with a plurality of phenotype identification windows. A first collector 205 is arranged at the phenotype identification window. The species data acquisition module 300 comprises a treatment liquid adding mechanism, a plurality of treatment bins 301, a sampling pump 302 and a second collector 303. The treatment liquid adding mechanism is used to add treatment liquid into the treatment bin 301. The treatment bin 301 supplies the detection end of the second collector 303 with a sample to be tested through a pipeline provided with the sampling pump 302. The temporary storage bin 102 is in communication with the concentrated liquid discharge port of the non-destructive dehydration and concentration mechanism 101 through a pipeline. The observation bin 202 is in one-to-one correspondence with the treatment bin 301 through a pipeline provided with a liquid supply pump. The first collector 205 and the second collector 303 are both in signal connection with the data processing and identification module 400.

[0032] It should be noted that the data processing recognition module can process the image, video or data information collected by the first and second collectors, and the computer software can complete the analysis, comparison or statistics of the information. Specifically, taking the image information processing to recognize the species of zooplankton as an example, the computer system has stored standard characteristic information of various zooplankton in advance, including body length, organ shape, etc. When the collector II detects the image of the zooplankton, the computer software automatically extracts the characteristic information of the image of the zooplankton and compares it with the standard characteristic information stored in advance. When the matching rate reaches the set value, the recognition is completed, that is, the automatic species recognition is realized. The graphic comparison can also be directly compared to recognize, and the specific algorithm is a mature technology in the art, which will not be described here.

[0033] In one embodiment of the present application, as shown in Figure 2 The lossless dehydration concentration mechanism 101 includes an outer shell 103 with an open upper end, a water containing barrel 104 and a dehydration barrel 105. The outer shell 103 is provided with an openable and closable cover 106 at the upper end. The water containing barrel 104 is fixedly arranged in the outer shell 103. The dehydration barrel 105 is located in the water containing barrel 104. A filter membrane is arranged on the inner wall of the dehydration barrel 105. A rotating shaft 107 penetrating the bottom wall of the water containing barrel 104 is transmissionally connected with a driving assembly 108 at the center of the bottom of the dehydration barrel 105. A flow guide channel is formed in the center of the rotating shaft 107. The lower end of the rotating shaft 107 is in communication with a concentrated liquid discharge pipe through a rotary sealing member. The concentrated liquid discharge pipe is in communication with the inlet of an external discharge pump 109. The outlet of the external discharge pump 109 is the concentrated liquid discharge outlet. The concentrated liquid discharge outlet is in communication with a temporary storage bin 102 through a pipeline. A water inlet mechanism 110 is arranged above the dehydration barrel 105. A water discharge mechanism 111 is arranged at the bottom of the water containing barrel 104. A backwashing nozzle 112 and a backblowing nozzle 113 are arranged between the water containing barrel 104 and the dehydration barrel 105.

[0034] Specifically, the water inlet mechanism sucks the target water body into the dehydration barrel, and after covering the cover, the driving assembly is started to drive the dehydration barrel to rotate for centrifugal dehydration. In order to avoid damage to plankton caused by high-speed centrifugation, the present application adopts low-speed dehydration. In order to not reduce the dehydration efficiency, the present application sets a back-blowing nozzle between the water barrel and the dehydration barrel. While rotating at low speed for dehydration, the back-blowing nozzle intermittently blows a certain pressure airflow from the outside to the filter membrane outside the dehydration barrel. The airflow back-blowing makes the plankton adhering to the filter membrane separate, so that the water more easily penetrates the filter membrane under the action of centrifugal force into the water barrel. The plankton timely separates from the filter membrane under the action of airflow back-blowing, and can also avoid long-term accumulation and damage to the plankton adhering to the filter membrane. When each concentration is completed or the filter membrane on the dehydration barrel needs to be cleaned, the backwashing nozzle can spray backwashing water from the outside to the filter membrane. Then the plankton adhering to the filter membrane can separate from the filter membrane and enter the concentrated liquid. The backwashing of the concentrated liquid should control the amount of backwashing water, and should not be too much, otherwise it will cause the concentrated liquid to be diluted.

[0035] The rotating seal can ensure that the rotating shaft rotates relative to the concentrated liquid discharge pipe and that the concentrated liquid does not leak from the gap between the rotating shaft flow guide channel and the concentrated liquid discharge pipe. The concentrated liquid discharge pipe is hard at least for a pipe section inserted into the flow guide channel, and an outer sleeve of the concentrated liquid discharge pipe is provided with the rotating seal.

[0036] On the basis of the above embodiment, the backwashing nozzle 112 communicates with the backwashing pump 114 outlet through a backwashing pipeline, the backwashing pump 114 inlet communicates with the clean water storage tank through a pipeline, the back-blowing nozzle 113 communicates with the air compressor 115 or the compressed gas storage tank through a back-blowing pipeline, and the backwashing pipeline and the back-blowing pipeline are respectively provided with electric valves.

[0037] It should be noted that the clean water storage tank can be arranged inside or outside the shell, and the clean water stored therein is preferably purified water treated by filtration and purification or distilled water treated by distillation. When the back-blowing nozzle delivers back-blowing gas through the air compressor, the air inlet end of the air compressor should be provided with a filtration and sterilization assembly to avoid dust, bacteria, viruses and the like in the air being blown onto the filter membrane.

[0038] On the basis of the above embodiment, a balance ring assembly 116 is arranged between the top of the dehydration barrel 105 and the top of the water barrel 104. The balance ring assembly is similar to the same kind of component used on a corrugated washing machine, and its function is to protect the dehydration barrel to rotate more stably.

[0039] On the basis of the above embodiment, a positioning sleeve 117 is detachably connected in the dehydration barrel 105. The sleeve wall of the positioning sleeve 117 is designed to be hollow and the filter membrane is tightly positioned on the inner wall of the dehydration barrel 105.

[0040] It should be noted that the positioning sleeve can be made of a certain elastic hard plastic or two half-circular sleeves spliced together, so that the positioning sleeve can be smoothly removed from the upper end of the dewatering barrel when the clamped filter membrane needs to be replaced. The size of the filter membrane is determined according to the size of the smallest individual of the zooplankton to be enriched.

[0041] On the basis of the above-mentioned embodiments, a liquid level sensor 118 is arranged on the inner wall of the dewatering barrel 105, the distance between the liquid level sensor 118 and the bottom wall of the dewatering barrel 105 is 5-8 cm, the space below the liquid level sensor 118 in the dewatering barrel 105 is a concentrated liquid collection chamber, and the side wall of the dewatering barrel 105 is densely provided with dewatering holes in the area above the concentrated liquid collection chamber.

[0042] It can be understood that the liquid level sensor is beneficial to automatically detecting the condition that the dewatering should be stopped and realizing automatic control. A water stirring tab can also be arranged on the bottom wall in the dewatering barrel to increase the ability to drive the liquid in the dewatering barrel to centrifuge when dewatering.

[0043] In an embodiment of the present application, as shown in Figure 3 The automatic sorting mechanism 201 includes a cylinder body provided with end covers at both ends, and a plurality of zooplankton collection nets 203 with mesh sizes decreasing in turn are arranged in the cylinder body in turn and at intervals from the inlet end to the outlet end. The fine end of each zooplankton collection net 203 is provided with an electromagnetic valve 204.

[0044] It should be noted that the automatic sorting mechanism is not limited to Figure 3 the structure shown in the figure, and an existing automatic sorting mechanism that has been publicly used can also be used, such as an automatic sorting device disclosed in Chinese invention patent CN201710287042, the output pipe of which is connected to a corresponding observation bin, and the sorting and observation of zooplankton can also be realized. In the present application, the working principle of the automatic sorting mechanism is that the zooplankton concentrate is transported to the automatic sorting mechanism and passes through the zooplankton collection nets in turn. At the beginning, the electromagnetic valves of all the collection nets are closed, and the liquid filtered through the automatic sorting mechanism no longer contains target zooplankton, and at this time, the liquid can be directly discharged into the environmental water body or the waste liquid pool. After a period of collection, the zooplankton of the corresponding size level is adhered and intercepted on each zooplankton collection net, and sorting is realized. At this time, the electromagnetic valve at the tail end of the collection net with the smallest mesh size (the rightmost in Figure 3 The concentrated liquid in the temporary storage bin can be used as a flushing liquid to supply liquid to the automatic sorting mechanism or a water pool (located Figure 3The clean water is sent to the automatic sorting mechanism, a valve on the pipeline in front of the observation bin is opened, the smallest target zooplankton is sent into the corresponding observation bin, then the electromagnetic valves of the collection nets are opened in order according to the gradually increasing mesh size, and the sorted zooplankton of the corresponding level is sent into the corresponding observation bin, so that the automatic sorting is realized. In the above process, since the concentrated solution contains various zooplankton, when the concentrated solution is used as the flushing liquid, a certain amount of the remaining zooplankton will be in the final large-size target zooplankton, but since the corresponding level of the collection net has previously enriched a large amount of zooplankton of the corresponding level, the liquid sent into the corresponding observation bin still has zooplankton of the corresponding level as the absolute majority, which basically does not affect the subsequent observation and information collection. Of course, if the clean water in the clean water tank is used as the flushing liquid, the effect is better.

[0045] On the basis of the above embodiment, the first collector 205 comprises a camera and / or a Doppler ultrasound monitor, and the second collector 303 comprises a camera, a photometer or a biological microscope.

[0046] It should be noted that the heart beat dynamics of the zooplankton within a set time are identified by means of Doppler ultrasound and the like, and this phenotype is mainly for large zooplankton with a heart, including cladocerans and calanoid copepods.

[0047] In an embodiment of the present application, as shown in Figure 5 The liquid adding mechanism comprises a liquid adding funnel 304, and the bottom of the liquid adding funnel 304 is provided with an electromagnetic valve 204 and a flow meter.

[0048] On the basis of the above embodiment, the liquid adding mechanism further comprises a fixed frame 305, a sliding block 306 and a sliding rail 307, the treatment bins 301 are arranged in line and adjacent to each other one by one, one sliding rail 307 is fixed to the same side of each treatment bin 301, one end of the fixed frame 305 is fixedly connected with the sliding block 306, the other end is fixedly connected with the liquid adding funnel 304, and when the sliding block 306 slides along the sliding rail 307, the liquid adding funnel 304 can pass above each treatment bin 301 in turn.

[0049] The above only describes the preferred embodiments of the present application and is not used to limit the present application, and any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. An integrated device for automatic zooplankton enrichment, physiological phenotype and species identification, characterized in that: The invention comprises a zooplankton enrichment module (100), a physiological phenotype data acquisition module (200), a species data acquisition module (300) and a data processing and identification module (400), wherein the zooplankton enrichment module (100) comprises a non-destructive dehydration and concentration mechanism (101) and a temporary storage chamber (102), the physiological phenotype data acquisition module (200) comprises an automatic sorting mechanism (201) and a plurality of observation chambers (202), the temporary storage chamber (102) is connected to each of the observation chambers (202) via the automatic sorting mechanism (201), each of the observation chambers (202) is provided with a plurality of phenotype identification windows, and a first collector (205) is provided at the phenotype identification window, and the species data acquisition module (300) comprises A treatment liquid adding mechanism, a plurality of treatment chambers (301), a sampling pump (302) and a second collector (303), wherein the treatment liquid adding mechanism is used to add treatment liquid into the treatment chamber (301), the treatment chamber (301) supplies a sample to be tested to the detection end of the second collector (303) through a pipeline provided with the sampling pump (302), the temporary storage chamber (102) is connected to the concentrated liquid discharge port of the non-destructive dehydration and concentration mechanism (101) through a pipeline, the observation chamber (202) is connected to the treatment chamber (301) in a one-to-one correspondence through a pipeline provided with a liquid supply pump, and the first collector (205) and the second collector (303) are both connected to the data processing and identification module (400) by signal.

2. The integrated device for automatic zooplankton enrichment, physiological phenotype and species identification according to claim 1, characterized in that: The lossless dehydration and concentration mechanism (101) comprises a shell (103) with an open top, a water storage barrel (104), and a dehydration barrel (105). The shell (103) is provided with an openable and closable cover (106) at its top. The water storage barrel (104) is fixedly disposed in the shell (103). The dehydration barrel (105) is located in the water storage barrel (104). A filter membrane is provided on the inner wall of the dehydration barrel (105). The center of the bottom of the dehydration barrel (105) is connected to a driving assembly (108) via a rotating shaft (107) that penetrates the bottom wall of the water storage barrel (104). The rotating shaft (107) A guide channel is provided at the center of the dewatering barrel (105); the lower end of the rotating shaft (107) is connected to a concentrated liquid discharge pipe through a rotating seal; the concentrated liquid discharge pipe is connected to the inlet of an external discharge pump (109); the outlet of the external discharge pump (109) is the concentrated liquid discharge outlet; the concentrated liquid discharge outlet is connected to the temporary storage bin (102) through a pipeline; a water inlet mechanism (110) is provided above the dewatering barrel (105); a drainage mechanism (111) is provided at the bottom of the water storage barrel (104); and a backwash nozzle (112) and a backflush nozzle (113) are provided between the water storage barrel (104) and the dewatering barrel (105).

3. The integrated device for automatic zooplankton enrichment, physiological phenotype and species identification according to claim 2, characterized in that: The backwash nozzle (112) is connected to the outlet of the backwash pump (114) through a backwash pipe, the inlet of the backwash pump (114) is connected to the clean water storage tank through a pipe, and the backflush nozzle (113) is connected to the air compressor (115) or the compressed gas storage tank through a backflush pipe. The backwash pipe and the backflush pipe are respectively provided with electric valves.

4. The integrated device for automatic zooplankton enrichment, physiological phenotype and species identification according to claim 2, characterized in that: A balance ring assembly (116) is provided between the top of the dehydration barrel (105) and the top of the water storage barrel (104).

5. The integrated device for automatic zooplankton enrichment, physiological phenotype and species identification according to claim 2, characterized in that: A positioning sleeve (117) is detachably connected to the dehydration barrel (105), and the wall of the positioning sleeve (117) is hollowed out and presses the filter membrane to position it on the inner wall of the dehydration barrel (105).

6. The integrated device for automatic zooplankton enrichment, physiological phenotype and species identification according to claim 2, characterized in that: A liquid level sensor (118) is provided on the inner wall of the dehydration barrel (105), and the distance between the liquid level sensor (118) and the bottom wall of the dehydration barrel (105) is 5-8 cm. The space below the liquid level sensor (118) in the dehydration barrel (105) is a concentrated liquid collecting chamber, and the side wall of the dehydration barrel (105) is densely covered with dehydration holes in the area above the concentrated liquid collecting chamber.

7. The integrated device for automatic zooplankton enrichment, physiological phenotype and species identification according to claim 1, characterized in that: The automatic sorting mechanism (201) comprises a cylinder with end covers at both ends, wherein multiple stages of zooplankton collecting nets (203) with mesh sizes decreasing in sequence are sequentially arranged in the cylinder from the inlet end to the outlet end, and the thin end of each zooplankton collecting net (203) is provided with a solenoid valve (204).

8. The integrated device for automatic zooplankton enrichment, physiological phenotype and species identification according to claim 1, characterized in that: The first collector (205) includes a camera and / or a Doppler ultrasound monitor, and the second collector (303) includes a camera, a still camera, or a biological microscope.

9. The integrated device for automatic zooplankton enrichment, physiological phenotype and species identification according to any one of claims 1 to 8, characterized in that: The treatment liquid adding mechanism comprises a liquid adding funnel (304), and a solenoid valve (204) and a flow meter are provided at the bottom of the liquid adding funnel (304).

10. The integrated device for automatic zooplankton enrichment, physiological phenotype and species identification according to claim 9, characterized in that: The treatment liquid adding mechanism also includes a fixed frame (305), a slider (306) and a slide rail (307). The treatment chambers (301) are arranged closely one by one along a straight line. One slide rail (307) is fixed on the same side of each treatment chamber (301). One end of the fixed frame (305) is fixedly connected to the slider (306), and the other end is fixedly connected to the liquid adding funnel (304). When the slider (306) slides along the slide rail (307), the liquid adding funnel (304) can pass over each treatment chamber (301) in turn.

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