Liquid electric resonance real-time label-free cell analyzer

Abstract

The invention discloses a liquid electric resonance real-time label-free cell analyzer, and relates to the technical field of cell analyzers, the liquid electric resonance real-time label-free cell analyzer comprises an analyzer main body, the outer side of the analyzer main body is provided with an automatic clamping mechanism, the automatic clamping mechanism comprises a movable frame, the inner wall of the movable frame is fixedly connected with a bearing, the inner ring of the bearing is fixedly connected with a stepping motor, and the stepping motor is fixedly connected with a motor shaft. The liquid electric resonance real-time label-free cell analyzer comprises an analyzer main body, a stepping motor is fixedly connected to the outer surface of the analyzer main body, a rotating column is fixedly connected to the output end of the stepping motor, two first sliding guide rails are fixedly connected to the outer surface of the analyzer main body, and a first electric telescopic rod is fixedly connected to the inner wall of each first sliding guide rail. The micro pump drives the pancreatin solution to be sprayed out, the cell culture plate can be soaked and washed in all directions, waste liquid is synchronously recycled to the waste liquid recycling box through the liquid suction head, a closed loop of cleaning and recycling is formed, the cell culture plate does not need to be manually disassembled, and residual sample pollution can be effectively avoided.

Description

Technical Field

[0001] This invention relates to the field of cell analyzer technology, specifically to a liquid electroresonance real-time label-free cell analyzer. Background Technology

[0002] Cell analyzers are biological instruments that use optical and electrical technologies to quantitatively detect cells. They are mainly used for cell counting, viability analysis, functional testing, and metabolic status monitoring, and are widely used in fields such as oncology research, CAR-T therapy monitoring, stem cell research, and drug toxicity testing.

[0003] Nowadays, after each experiment, cell analyzers often require staff to remove the cell culture plates from the instrument and then clean and disinfect them. During this process, experiments cannot continue, which reduces experimental efficiency. Furthermore, manual cleaning cannot effectively remove residual cell samples and impurities, which can easily cause cross-contamination and reduce experimental accuracy.

[0004] Combining the above issues, we find that existing cell analyzers are difficult to simultaneously avoid these problems during use. Even if they can be solved, they require external tools, thus failing to achieve the desired results. Therefore, we propose a liquid electroresonance real-time label-free cell analyzer. Summary of the Invention

[0005] The purpose of this invention is to provide a real-time label-free cell analyzer based on liquid electroresonance to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a real-time label-free liquid electroresonance cell analyzer, comprising an analyzer body, an automatic clamping mechanism disposed on the outer side of the analyzer body, the automatic clamping mechanism comprising a movable frame, a bearing fixedly connected to the inner wall of the movable frame, a stepper motor fixedly connected to the inner ring of the bearing, a rotating column fixedly connected to the output end of the stepper motor, two first sliding guide rails fixedly connected to the outer surface of the analyzer body, a first electric telescopic rod fixedly connected to the inner wall of each first sliding guide rail, a first sliding block fixedly connected to the telescopic end of each first electric telescopic rod, the upper surfaces of the two first sliding blocks being fixedly connected to the bottom surface of the movable frame, a second electric telescopic rod fixedly connected to the bottom end of the rotating column, and a clamping mechanical claw fixedly connected to the telescopic end of the second electric telescopic rod;

[0007] An automatic cleaning and disinfection assembly is installed on the outside of the analyzer body, and a protective assembly is installed inside the analyzer body.

[0008] Preferably, the automatic cleaning and disinfection assembly includes two sliding frames, each with a micro pump fixedly connected to its upper surface. A collection box is fixedly connected to the back of the analyzer body, and two second sliding guide rails are fixedly connected to the back of the collection box. A third electric telescopic rod is fixedly connected to the inner wall of each second sliding guide rail, and a second sliding block is fixedly connected to the telescopic end of each third electric telescopic rod. The upper surface of each second sliding block is fixedly connected to the bottom surface of the sliding frame. A fourth electric telescopic rod is fixedly connected to the inner wall of each sliding frame, and a moving block is fixedly connected to the telescopic end of each fourth electric telescopic rod. Each block has a connecting frame fixedly connected to its front side, and a fixing ring fixedly connected to the outer surface of each connecting frame. Each fixing ring has an annular tube fixedly connected to its bottom end. One annular tube has a suction head fixedly connected to its outer surface, and another annular tube has a spray head fixedly connected to its outer surface. The bottom surface of the collection box has a waste liquid recovery box and a pancreatic enzyme solution storage box fixedly connected to it. The output end of one micropump and the input end of another micropump are fixedly connected to a first telescopic hose, and the input end of one micropump and the output end of another micropump are fixedly connected to a second telescopic hose. The automatic cleaning and disinfection assembly is also equipped with an ultraviolet irradiation lamp.

[0009] Preferably, the protective assembly includes two protective baffles, two first push rods, two second push rods, and four connecting rods. Two fixing plates are fixedly connected to the bottom surface of each protective baffle. Two first limiting rods are fixedly connected to the side of each group of fixing plates that are close to each other. Two first fixing rods, four second fixing rods, and two third fixing rods are fixedly connected to the inner wall of the analyzer body. A rotating block is movably connected to the outer surface of each third fixing rod. A supporting block is movably connected to the outer surface of each first fixing rod. Two movable blocks are movably connected to the outer surface of each group of second fixing rods. Each group of movable blocks is close to each other. Four second limiting rods are fixedly connected to the side of each of the two rotating blocks that are close to each other. Two third limiting rods are fixedly connected to the side of each of the two rotating blocks that are close to each other. The inner wall of each first pushing rod is in contact with the outer surface of one of the second limiting rods and the outer surface of one of the third limiting rods. The inner wall of each second pushing rod is in contact with the outer surface of the other second limiting rod and the other third limiting rod. A fifth electric telescopic rod is fixedly connected to the back of each of the supporting blocks. A movable frame is fixedly connected to the telescopic end of each fifth electric telescopic rod. The inner wall of each movable frame is in contact with the outer surface of one of the third limiting rods.

[0010] Preferably, a protective box is fixedly connected to the outer surface of the stepper motor, and the bottom surface of the protective box is fixedly connected to the upper surface of the movable frame.

[0011] Preferably, a control panel is fixedly connected to the front of the analyzer body, a fixing frame is fixedly connected to the outer surface of the control panel, the back of the fixing frame is fixedly connected to the front of the analyzer body, a measurement module is fixedly connected to the inner wall of the analyzer body, a limit ring is fixedly connected to the upper surface of the measurement module, a cell culture plate is snapped into the inside of the limit ring, a sensing electrode chip is fixedly connected to the outer surface of the cell culture plate, and the measurement module is electrically connected to the control panel through wires.

[0012] Preferably, a protective seat is fixedly connected to the outer surface of the second electric telescopic rod, and the upper surface of the protective seat is fixedly connected to the inner wall of the movable frame.

[0013] Preferably, a reinforcing plate is fixedly connected to the outer surface of the telescopic end of the second electric telescopic rod, and the bottom surface of the reinforcing plate is fixedly connected to the upper surface of the clamping mechanical claw.

[0014] Preferably, the inner wall of the reinforcing plate is threaded with four fixing bolts, and the outer surface of each set of fixing bolts is threadedly connected to the inner wall of the clamping mechanical claw.

[0015] Preferably, each of the micro pumps has a support frame fixedly connected to its outer surface, and the bottom surface of each support frame is fixedly connected to the upper surface of the sliding frame.

[0016] Preferably, the inner wall of the waste liquid recovery box and the inner wall of the pancreatic enzyme solution storage box are both fitted with rubber stoppers, and the bottom end of each second telescopic hose is fixedly connected to the outer surface of the annular tube.

[0017] Compared with the prior art, the beneficial effects of the present invention are:

[0018] 1. This invention uses a first electric telescopic rod to drive the movable frame to move up and down along the first sliding guide rail, and a stepper motor to drive the rotating column to rotate. This allows for precise adjustment of the position of the clamping mechanical claw, enabling automatic gripping, transfer, and placement of cell culture plates without the need for manual contact with the samples, thus avoiding operational errors.

[0019] 2. This invention features a nozzle and a ring tube connected to a pancreatic enzyme solution storage box. A micro-pump drives the pancreatic enzyme solution to spray out, which can thoroughly soak and rinse the cell culture plate. Then, it is disinfected and sterilized by ultraviolet light irradiation. The waste liquid is simultaneously collected to the waste liquid collection box using a suction head, forming a closed loop of cleaning and recycling. There is no need to manually disassemble the cell culture plate, avoiding contamination by residual samples.

[0020] 3. By providing a protective baffle, the present invention can block external dust or accidental collisions during instrument operation, protecting the internal detection components. The movable connection between the rotating block and the third fixed rod, in conjunction with the fifth electric telescopic rod driving the moving frame, allows the protective baffle to be expanded or retracted as needed, enhancing the applicability of the device. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 This is a schematic diagram of the structure of the first electric telescopic rod and the first sliding block of the present invention;

[0023] Figure 3 This is a schematic diagram of the structure of the rotating column and the second electric telescopic rod of the present invention;

[0024] Figure 4 This is a schematic diagram of the sliding frame and collection box of the present invention;

[0025] Figure 5 This is a schematic diagram of the structure of the waste liquid recovery box and the pancreatic enzyme solution storage box of the present invention;

[0026] Figure 6 This is a schematic diagram of the structure of the fixing ring and the annular tube of the present invention;

[0027] Figure 7 This is a schematic diagram of the structure of the fourth electric telescopic rod and the moving block of the present invention;

[0028] Figure 8 This is a schematic diagram of the rotating block and the fifth electric telescopic rod of the present invention;

[0029] Figure 9 This is a schematic diagram of the structure of the fixed plate and the movable block of the present invention;

[0030] Figure 10 This is a schematic diagram of the structure of the third limiting rod and the third fixing rod of the present invention;

[0031] Figure 11 This is a schematic diagram of the structure of the fixed plate and the movable block of the present invention;

[0032] Figure 12 This is a schematic diagram of the internal structure of the analyzer body of the present invention.

[0033] In the picture:

[0034] 1. Main body of the analyzer;

[0035] 2. Automatic clamping mechanism; 201. Movable frame; 202. First sliding guide rail; 203. Stepper motor; 204. Bearing; 205. First electric telescopic rod; 206. First sliding block; 207. Rotating column; 208. Second electric telescopic rod; 209. Protective seat; 210. Reinforcing plate; 211. Fixing bolt; 212. Clamping claw; 213. Control panel; 214. Fixing frame; 215. Protective box; 216. Cell culture plate; 217. Sensing electrode chip; 218. Measurement module; 219. Limiting ring;

[0036] 3. Automatic cleaning and disinfection components; 301. Sliding frame; 302. Collection box; 303. Micro pump; 304. Waste liquid recovery box; 305. Pancreatic enzyme solution storage box; 306. Second sliding guide rail; 307. First telescopic hose; 308. Support frame; 309. Second telescopic hose; 310. Fixing ring; 311. Ring tube; 312. Suction head; 313. Connecting frame; 314. Third electric telescopic rod; 315. Second sliding block; 316. Fourth electric telescopic rod; 317. Moving block; 318. Spray head; 319. Rubber stopper;

[0037] 4. Protective components; 401. Protective baffle; 402. Rotating block; 403. Fifth electric telescopic rod; 404. Fixed plate; 405. Movable block; 406. First push rod; 407. First fixed rod; 408. Movable frame; 409. Second fixed rod; 410. Connecting rod; 411. First limit rod; 412. Second limit rod; 413. Third limit rod; 414. Third fixed rod; 415. Support block; 416. Second push rod. Detailed Implementation

[0038] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0039] Example 1: Please refer to Figures 1-3 This invention provides a technical solution: a real-time label-free liquid electroresonance cell analyzer, comprising an analyzer body 1, an automatic clamping mechanism 2 on the outer side of the analyzer body 1, the automatic clamping mechanism 2 including a movable frame 201, a bearing 204 fixedly connected to the inner wall of the movable frame 201, a stepper motor 203 fixedly connected to the inner ring of the bearing 204, a rotating column 207 fixedly connected to the output end of the stepper motor 203, two first sliding guide rails 202 fixedly connected to the outer surface of the analyzer body 1, a first electric telescopic rod 205 fixedly connected to the inner wall of each first sliding guide rail 202, a first sliding block 206 fixedly connected to the telescopic end of each first electric telescopic rod 205, the upper surfaces of the two first sliding blocks 206 being fixedly connected to the bottom surface of the movable frame 201, a second electric telescopic rod 208 fixedly connected to the bottom end of the rotating column 207, and a clamping mechanical claw 212 fixedly connected to the telescopic end of the second electric telescopic rod 208.

[0040] A protective box 215 is fixedly connected to the outer surface of the stepper motor 203. The bottom surface of the protective box 215 is fixedly connected to the upper surface of the movable frame 201. The protective box 215 can protect the stepper motor 203 and prevent it from being damaged by the outside during use.

[0041] A control panel 213 is fixedly connected to the front of the analyzer body 1. A fixing frame 214 is fixedly connected to the outer surface of the control panel 213. The back of the fixing frame 214 is fixedly connected to the front of the analyzer body 1. A measurement module 218 is fixedly connected to the inner wall of the analyzer body 1. A limit ring 219 is fixedly connected to the upper surface of the measurement module 218. A cell culture plate 216 is snapped into the inside of the limit ring 219. A sensing electrode chip 217 is fixedly connected to the outer surface of the cell culture plate 216. The measurement module 218 is electrically connected to the control panel 213 via wires. The control panel 213 facilitates the control of the device. The fixing frame 214 can fix the control panel 213, enhancing the stability of the device. The cell culture plate 216 uses a cross-shaped induction electrode chip 217, which is gold-plated, to ensure high accuracy, high repeatability and normal cell growth of cell detection. The measurement module 218 can extract the electrical resonance frequency of the liquid, effectively select the resonance frequency of the liquid in the cell culture plate, accurately measure the frequency signal, and finally transmit the measurement results to the control panel 213.

[0042] The outer surface of the second electric telescopic rod 208 is fixedly connected to a protective seat 209. The upper surface of the protective seat 209 is fixedly connected to the inner wall of the movable frame 201. The protective seat 209 can protect the second electric telescopic rod 208 and prevent it from being interfered with by external forces during use.

[0043] A reinforcing plate 210 is fixedly connected to the outer surface of the telescopic end of the second electric telescopic rod 208. The bottom surface of the reinforcing plate 210 is fixedly connected to the upper surface of the clamping mechanical claw 212. Through the reinforcing plate 210, the connection between the second electric telescopic rod 208 and the clamping mechanical claw 212 can be reinforced to prevent it from swaying during use.

[0044] The inner wall of the reinforcing plate 210 is threaded with four fixing bolts 211. The outer surface of each set of fixing bolts 211 is threaded with the inner wall of the clamping mechanical claw 212. The fixing bolts 211 can restrict the position of the reinforcing plate 210 and play a role in fixing and limiting it.

[0045] The specific implementation of this embodiment is as follows: When it is necessary to grasp the cell culture plate, the first electric telescopic rod 205 is activated through the control panel 213. Its telescopic end pushes the first sliding block 206 to move horizontally within the first sliding guide rail 202, causing the movable frame 201 to move horizontally along the outside of the analyzer body 1. Then, the second electric telescopic rod 208 is lowered, so that the gripping mechanical claw 212 grasps the cell culture plate. The height of the gripping mechanical claw 212 can be precisely adjusted. At the same time, the stepper motor 203 drives the rotating column 207 to rotate through the bearing 204, so that the gripping mechanical claw 212 rotates in the horizontal direction, realizing the position calibration in three-dimensional space. Then, the cell culture plate is transferred to the detection area of ​​the analyzer body 1 through the first electric telescopic rod 205 and the stepper motor 203. There is no need for manual contact with the sample, reducing operation errors and achieving the purpose of automatically picking up the cell culture plate.

[0046] Example 2: Please refer to Figure 1 , Figures 4-7 The present invention provides a technical solution: a liquid electroresonance real-time label-free cell analyzer. The present invention makes corresponding improvements to the technical problems mentioned in the background art. An automatic cleaning and disinfection component 3 is provided on the outside of the analyzer body 1.

[0047] As a further definition of the automatic cleaning and disinfection assembly 3 of the present invention, the automatic cleaning and disinfection assembly 3 includes two sliding frames 301, each with a micro pump 303 fixedly connected to its upper surface. A collection box 302 is fixedly connected to the back of the analyzer body 1. Two second sliding guide rails 306 are fixedly connected to the back of the collection box 302. A third electric telescopic rod 314 is fixedly connected to the inner wall of each second sliding guide rail 306. A second sliding block 315 is fixedly connected to the telescopic end of each third electric telescopic rod 314. The upper surface of each second sliding block 315 is fixedly connected to the bottom surface of the sliding frame 301. A fourth electric telescopic rod 316 is fixedly connected to the inner wall of each sliding frame 301. The telescopic end of each fourth electric telescopic rod 316 is fixedly connected to... Each movable block 317 is connected to a connecting frame 313, and a fixing ring 310 is fixedly connected to the outer surface of each connecting frame 313. An annular tube 311 is fixedly connected to the bottom end of each fixing ring 310. A suction head 312 is fixedly connected to the outer surface of one annular tube 311, and a nozzle 318 is fixedly connected to the outer surface of the other annular tube 311. A waste liquid recovery box 304 and a pancreatic enzyme solution storage box 305 are fixedly connected to the bottom surface of the collection box 302. A first telescopic hose 307 is fixedly connected to the output end of one micro pump 303 and the input end of another micro pump 303. A second telescopic hose 309 is fixedly connected to the input end of one micro pump 303 and the output end of another micro pump 303.

[0048] Each micro pump 303 has a support frame 308 fixedly connected to its outer surface. The bottom surface of each support frame 308 is fixedly connected to the upper surface of the sliding frame 301. The support frame 308 can fix and support the micro pump 303 to prevent its position from shifting and becoming unstable during use.

[0049] The automatic cleaning and disinfection component 3 is also equipped with an ultraviolet irradiation lamp. By setting the ultraviolet irradiation lamp, it can play a role in disinfection and sterilization, ensuring aseptic operation.

[0050] Rubber stoppers 319 are snapped into the inner walls of the waste liquid recovery box 304 and the pancreatic enzyme solution storage box 305. The bottom end of each second telescopic hose 309 is fixedly connected to the outer surface of the annular tube 311. The rubber stoppers 319 can protect the liquid in the waste liquid recovery box 304 and the pancreatic enzyme solution storage box 305 from external contamination.

[0051] The specific implementation of this embodiment is as follows: When the cell culture plate needs to be cleaned, the telescopic end of the third electric telescopic rod 314 is extended or retracted, which drives the second sliding block 315 to move, thereby adjusting the position of the sliding frame 301. This allows for the adjustment of the positions of the nozzle 318 and the suction head 312. The micro pump 303 and the second telescopic hose 309 draw trypsin solution from the trypsin solution storage box 305, deliver it to the ring tube 311, and then spray it out from the nozzle 318 to thoroughly soak and rinse the cell culture plate. Then, the plate is disinfected and sterilized by irradiation with an ultraviolet lamp. After soaking and rinsing, another micro pump 303 and the first telescopic hose 307 draw the waste liquid collected by the suction head 312 into the waste liquid recovery box 304, forming a closed loop of soaking, rinsing, and recovery. This avoids residual contamination caused by manual disassembly of the cell culture plate and improves experimental efficiency.

[0052] Example 3: Please refer to Figure 1 , Figures 8-12 The present invention provides a technical solution: a liquid electroresonance real-time label-free cell analyzer. The present invention makes corresponding improvements to the technical problems mentioned in the background art. The analyzer body 1 is provided with a protective component 4 inside.

[0053] As a further definition of the protective component 4 of the present invention, the protective component 4 includes two protective baffles 401, two first pushing rods 406, two second pushing rods 416, and four connecting rods 410. Two fixing plates 404 are fixedly connected to the bottom surface of each protective baffle 401. Two first limiting rods 411 are fixedly connected to the side of each set of fixing plates 404 that are close to each other. Two first fixing rods 407, four second fixing rods 409, and two third fixing rods 414 are fixedly connected to the inner wall of the analyzer body 1. A rotating block 402 is movably connected to the outer surface of each third fixing rod 414. A support block 415 is movably connected to the outer surface of each first fixing rod 407. Two movable blocks 405 are movably connected to the outer surface of each set of second fixing rods 409. Each movable block 405 has four second limiting rods 412 fixedly connected to one side of each other. Each rotating block 402 has two third limiting rods 413 fixedly connected to one side of each other. The inner wall of each first pushing rod 406 is in contact with the outer surface of one of the second limiting rods 412 and the outer surface of one of the third limiting rods 413. The inner wall of each second pushing rod 416 is in contact with the outer surface of the other second limiting rod 412 and the other third limiting rod 413. Each support block 415 has a fifth electric telescopic rod 403 fixedly connected to its back. Each telescopic end of the fifth electric telescopic rod 403 is fixedly connected to a moving frame 408. The inner wall of each moving frame 408 is in contact with the outer surface of one of the third limiting rods 413.

[0054] The specific implementation of this embodiment is as follows: When the device needs to be deployed for testing, the fifth electric telescopic rod 403 drives the moving frame 408 to move, causing the rotating block 402 to rotate around the third fixed rod 414, pushing the first push rod 406 and the second push rod 416, thereby driving the movable block 405 to rotate, driving the fixed plate 404 to move, so that the protective baffle 401 is deployed, covering the internal detection components of the analyzer body 1 to prevent dust or accidental collisions. When the baffle needs to be retracted, the fifth electric telescopic rod 403 is retracted, the moving frame 408 drives the rotating block 402 to rotate in the opposite direction, and the protective baffle 401 is retracted through linkage.

[0055] Specifically, this liquid electroresonance real-time label-free cell analyzer operates / is used as follows:

[0056] First, when it is necessary to grasp the cell culture plate, the first electric telescopic rod 205 is activated through the control panel 213. Its telescopic end pushes the first sliding block 206 to move horizontally within the first sliding guide rail 202, causing the movable frame 201 to move horizontally along the outside of the analyzer body 1. Then, the second electric telescopic rod 208 is lowered, so that the gripping mechanical claw 212 grasps the cell culture plate. The height of the gripping mechanical claw 212 can be precisely adjusted. At the same time, the stepper motor 203 drives the rotating column 207 to rotate through the bearing 204, so that the gripping mechanical claw 212 rotates in the horizontal direction, realizing the position calibration in three-dimensional space. Then, the cell culture plate is transferred to the detection area of ​​the analyzer body 1 through the first electric telescopic rod 205 and the stepper motor 203. There is no need for manual contact with the sample, reducing operation error and achieving the purpose of automatically picking up the cell culture plate.

[0057] Then, when the cell culture plate needs to be cleaned, the second sliding block 315 can be moved by extending and retracting the extension end of the third electric telescopic rod 315, thereby adjusting the position of the sliding frame 301. This allows for the adjustment of the positions of the nozzle 318 and the suction head 312. The trypsin solution is drawn from the trypsin solution storage box 305 by the micro pump 303 and the second telescopic hose 309, delivered to the ring tube 311, and then sprayed out from the nozzle 318 to thoroughly soak and rinse the cell culture plate. After soaking and rinsing, the waste liquid collected by the suction head 312 is sucked into the waste liquid recovery box 304 by another micro pump 303 and the first telescopic hose 307, forming a closed loop of soaking, rinsing, and recovery. This avoids residual contamination caused by manual disassembly of the cell culture plate and improves experimental efficiency.

[0058] Furthermore, when the device needs to be deployed for experiments, the fifth electric telescopic rod 403 drives the moving frame 408 to move, causing the rotating block 402 to rotate around the third fixed rod 414, pushing the first push rod 406 and the second push rod 416, thereby driving the movable block 405 to rotate, causing the fixed plate 404 to move, so that the protective baffle 401 unfolds, shielding the internal detection components of the analyzer body 1 to prevent dust or accidental collisions. When the baffle needs to be retracted, the fifth electric telescopic rod 403 retracts, the moving frame 408 drives the rotating block 402 to rotate in the opposite direction, and the protective baffle 401 retracts through linkage.

[0059] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0060] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A real-time label-free cell analyzer based on liquid electroresonance, comprising an analyzer body (1), characterized in that: An automatic clamping mechanism (2) is provided on the outer side of the analyzer body (1). The automatic clamping mechanism (2) includes a movable frame (201). A bearing (204) is fixedly connected to the inner wall of the movable frame (201). A stepper motor (203) is fixedly connected to the inner ring of the bearing (204). A rotating column (207) is fixedly connected to the output end of the stepper motor (203). Two first sliding guide rails (202) are fixedly connected to the outer surface of the analyzer body (1). Each first sliding guide rail... The inner wall of the sliding guide rail (202) is fixedly connected with a first electric telescopic rod (205), and the telescopic end of each first electric telescopic rod (205) is fixedly connected with a first sliding block (206). The upper surfaces of the two first sliding blocks (206) are fixedly connected to the bottom surface of the movable frame (201). The bottom end of the rotating column (207) is fixedly connected with a second electric telescopic rod (208), and the telescopic end of the second electric telescopic rod (208) is fixedly connected with a clamping mechanical claw (212). An automatic cleaning and disinfection component (3) is provided on the outside of the analyzer body (1), and a protective component (4) is provided inside the analyzer body (1).

2. The real-time label-free cell analyzer based on liquid electroresonance according to claim 1, characterized in that: The automatic cleaning and disinfection assembly (3) includes two sliding frames (301). A micro pump (303) is fixedly connected to the upper surface of each sliding frame (301). A collection box (302) is fixedly connected to the back of the analyzer body (1). Two second sliding guide rails (306) are fixedly connected to the back of the collection box (302). A third electric telescopic rod (314) is fixedly connected to the inner wall of each second sliding guide rail (306). A second sliding block (315) is fixedly connected to the telescopic end of each third electric telescopic rod (314). The upper surface of each second sliding block (315) is fixedly connected to the bottom surface of the sliding frame (301). A fourth electric telescopic rod (316) is fixedly connected to the inner wall of each sliding frame (301). A moving block (317) is fixedly connected to the telescopic end of each fourth electric telescopic rod (316). The upper surface of each moving block (317) is fixedly connected to the bottom surface of the sliding frame (301). Each of the three components is fixedly connected to a connecting frame (313). Each connecting frame (313) is fixedly connected to a fixing ring (310) on its outer surface. Each fixing ring (310) is fixedly connected to a ring tube (311) at its bottom end. One of the ring tubes (311) is fixedly connected to a suction head (312) on its outer surface. The other ring tube (311) is fixedly connected to a nozzle (318) on its outer surface. The bottom surface of the collection box (302) is fixedly connected to a waste liquid recovery box (304) and a pancreatic enzyme solution storage box (305). The output end of one of the micro pumps (303) and the input end of the other micro pump (303) are fixedly connected to a first telescopic hose (307). The input end of one of the micro pumps (303) and the output end of the other micro pump (303) are fixedly connected to a second telescopic hose (309). The automatic cleaning and disinfection component (3) is also equipped with an ultraviolet irradiation lamp inside.

3. The real-time label-free cell analyzer based on liquid electroresonance according to claim 1, characterized in that: The protective assembly (4) includes two protective baffles (401), two first push rods (406), two second push rods (416), and four connecting rods (410). Two fixed plates (404) are fixedly connected to the bottom surface of each protective baffle (401). Two first limiting rods (411) are fixedly connected to the side of each set of fixed plates (404) that are close to each other. Two first fixing rods (407), four second fixing rods (409), and two third fixing rods (414) are fixedly connected to the inner wall of the analyzer body (1). A rotating block (402) is movably connected to the outer surface of each third fixing rod (414). A support block (415) is movably connected to the outer surface of each first fixing rod (407). Two movable blocks (405) are movably connected to the outer surface of each set of second fixing rods (409). (405) Four second limiting rods (412) are fixedly connected to each other on one side that is close to each other. Two third limiting rods (413) are fixedly connected to each other on one side that is close to each other. The inner wall of each first pushing rod (406) is in contact with the outer surface of one of the second limiting rods (412) and the outer surface of one of the third limiting rods (413). The inner wall of each second pushing rod (416) is in contact with the outer surface of the other second limiting rod (412) and the outer surface of the other third limiting rod (413). A fifth electric telescopic rod (403) is fixedly connected to the back of each support block (415). A moving frame (408) is fixedly connected to the telescopic end of each fifth electric telescopic rod (403). The inner wall of each moving frame (408) is in contact with the outer surface of one of the third limiting rods (413).

4. The real-time label-free cell analyzer based on liquid electroresonance according to claim 1, characterized in that: A protective box (215) is fixedly connected to the outer surface of the stepper motor (203), and the bottom surface of the protective box (215) is fixedly connected to the upper surface of the movable frame (201).

5. The real-time label-free cell analyzer based on liquid electroresonance according to claim 1, characterized in that: A control panel (213) is fixedly connected to the front of the analyzer body (1). A fixing frame (214) is fixedly connected to the outer surface of the control panel (213). The back of the fixing frame (214) is fixedly connected to the front of the analyzer body (1). A measurement module (218) is fixedly connected to the inner wall of the analyzer body (1). A limiting ring (219) is fixedly connected to the upper surface of the measurement module (218). A cell culture plate (216) is snapped into the inside of the limiting ring (219). A sensing electrode chip (217) is fixedly connected to the outer surface of the cell culture plate (216). The measurement module (218) is electrically connected to the control panel (213) through wires.

6. The real-time label-free cell analyzer based on liquid electroresonance according to claim 1, characterized in that: The outer surface of the second electric telescopic rod (208) is fixedly connected to a protective seat (209), and the upper surface of the protective seat (209) is fixedly connected to the inner wall of the movable frame (201).

7. The real-time label-free cell analyzer based on liquid electroresonance according to claim 1, characterized in that: A reinforcing plate (210) is fixedly connected to the outer surface of the telescopic end of the second electric telescopic rod (208), and the bottom surface of the reinforcing plate (210) is fixedly connected to the upper surface of the clamping mechanical claw (212).

8. A real-time label-free cell analyzer based on liquid electroresonance according to claim 7, characterized in that: The inner wall of the reinforcing plate (210) is threaded with four fixing bolts (211), and the outer surface of each set of fixing bolts (211) is threadedly connected to the inner wall of the clamping mechanical claw (212).

9. A real-time label-free cell analyzer based on liquid electroresonance according to claim 2, characterized in that: Each of the micro pumps (303) has a support frame (308) fixedly connected to its outer surface, and the bottom surface of each support frame (308) is fixedly connected to the upper surface of the sliding frame (301).

10. A real-time label-free cell analyzer based on liquid electroresonance according to claim 2, characterized in that: The inner wall of the waste liquid recovery box (304) and the inner wall of the pancreatic enzyme solution storage box (305) are both fitted with rubber stoppers (319), and the bottom end of each of the second telescopic hoses (309) is fixedly connected to the outer surface of the annular tube (311).

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