Fish peptide compound with antioxidant, anti-wrinkle and whitening functions as well as preparation method and application of fish peptide compound

Abstract

The invention discloses a fish peptide compound with anti-oxidation, anti-wrinkle and whitening functions as well as a preparation method and application thereof, belongs to the technical field of functional peptide application, and provides the fish peptide compound with the anti-oxidation, anti-wrinkle and whitening functions. Comprising 40 to 50 parts of fish collagen peptide, 36 to 40 parts of elastin peptide, 18 to 21 parts of collagen tripeptide, 14 to 17 parts of tuna active peptide, 5 to 6 parts of avocado extract, 2 to 4 parts of fish peptide and 2.6 to 3.0 parts of rye seed extract. According to the fish peptide compound with the antioxidant, anti-wrinkle and whitening functions as well as the preparation method and the application, the fish-derived functional peptide and the plant extract are reasonably compounded, all the components play a synergistic role, and the prepared fish peptide compound has the antioxidant, anti-wrinkle and whitening effects at the same time.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of functional peptide application, and particularly relates to a fish peptide compound with antioxidant anti-wrinkle whitening function, a preparation method and application thereof. BACKGROUND

[0002] With the growth of age, the skin will undergo natural aging process, and at the same time, be affected by environmental factors such as ultraviolet radiation, pollution, etc., leading to increased oxidative stress. This oxidative stress can accelerate skin aging, manifesting as increased wrinkles, decreased elasticity and pigmentation. The current market antioxidants and whitening ingredients can alleviate skin aging and pigmentation to some extent, but often have problems such as low absorption rate, poor stability or short-lasting effect.

[0003] Fish peptides have been widely concerned in the field of skin care due to their good biocompatibility and high antioxidant capacity. Studies have shown that fish peptides not only can scavenge free radicals, but also can promote collagen synthesis, thereby improving skin elasticity and reducing wrinkles. For example, tilapia skin peptides (IL, FGP, LPGL) prepared by enzymatic hydrolysis and solid-phase chemical synthesis exhibit significant antioxidant activity and cell repair capacity, and are suitable for anti-aging products. The use of fish peptides with other functional ingredients can play a synergistic effect and enhance the antioxidant, anti-wrinkle and whitening effects. For example, fish-derived collagen antioxidant peptides can enhance the extensibility and thermal stability of collagen when cross-linked with bletilla striata gum, and are used in beauty masks. However, there is still a lack of systematic research on the specific preparation method and application of fish peptide compounds.

[0004] In summary, the existing technology provides a basis for the development of fish peptide compounds, but still needs to solve the problems of preparation efficiency, functional integration and product stability, etc. to meet the multiple needs of antioxidant, anti-wrinkle and whitening. SUMMARY

[0005] To solve the above technical problems, the present application provides a fish peptide compound with antioxidant anti-wrinkle whitening function, a preparation method and application thereof. By reasonably compounding fish-derived functional peptides and plant extracts, each component plays a synergistic role, and the prepared fish peptide compound has antioxidant, anti-wrinkle and whitening effects.

[0006] To achieve the above purpose, the present application provides a fish peptide compound with antioxidant anti-wrinkle whitening function, comprising the following components by weight: 40-50 parts of fish collagen peptide, 36-40 parts of elastin peptide, 18-21 parts of collagen tripeptide, 14-17 parts of tuna active peptide, 5-6 parts of avocado extract, 2-4 parts of fish peptide, and 2.6-3.0 parts of rye seed extract.

[0007] Preferably, the following components are included by weight: fish collagen peptides 45 parts, elastin peptides 38 parts, collagen tripeptides 19.5 parts, tuna active peptides 15.5 parts, avocado extract 5.5 parts, fish peptigen 3 parts, rye seed extract 2.8 parts.

[0008] Fish collagen peptides: Small molecule collagen extracted from fish, more easily absorbed by the human body after hydrolysis. Its main functions include improving skin elasticity, moisturizing, promoting repair, and may also have certain auxiliary effects on joint and bone health. Amino acids such as glycine and proline in fish collagen peptides are important components of the dermis layer of the skin, which can help maintain skin structure and reduce fine lines and dryness. Studies have shown that continuous oral supplementation may increase skin water content. Fish collagen peptides contain antioxidant components that may help reduce free radical damage.

[0009] Elastin peptides: Directly acting on the deep layer of the skin, stimulating fibroblasts to secrete elastin and collagen, enhancing the support force of the skin network structure, thereby reducing wrinkles and improving firmness. Studies have shown that long-term supplementation of elastin peptides can improve skin water content and elasticity, and delay the relaxation problems caused by ultraviolet rays and age. Elastin is an important component of human connective tissue, containing specific amino acids such as valine and proline, which can neutralize free radicals and reduce oxidative stress damage to the skin. In addition, its anti-inflammatory properties can alleviate skin redness and sensitivity caused by ultraviolet rays or external stimuli. Elastin peptides enhance tissue resilience, delay skin relaxation and collapse.

[0010] Collagen tripeptides: A small molecule collagen fragment, its main functions are to promote the synthesis of skin's own collagen, improve skin elasticity, and assist in antioxidant and moisturizing. Its molecular weight is smaller, and it is more easily absorbed by the human body than ordinary collagen, making it suitable as an oral or external anti-aging ingredient. Collagen tripeptides can directly act on skin fibroblasts, activate collagen synthesis signaling pathways (such as TGF-β), and help reduce fine lines and improve relaxation. Hydroxyproline contained in collagen tripeptides can neutralize free radicals, reduce oxidative damage caused by ultraviolet rays, and inhibit the formation of advanced glycation end products (AGEs), delaying skin yellowing and roughness. By increasing the content of hyaluronic acid in the dermis layer, it improves the water retention capacity of the epidermis, and improves dryness, sensitivity and other problems. When used externally, it can form a breathable protective film and reduce water loss.

[0011] Tuna active peptides: are short-chain amino acid combinations produced by enzymatic or fermentation of tuna protein, with multiple physiological regulation functions, mainly including antioxidant, blood pressure regulation, immune enhancement, anti-fatigue, and skin health promotion. Tuna active peptides can scavenge free radicals and reduce oxidative stress damage to cells. With small molecular weight, they are easily absorbed by the human body, improve the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), reduce lipid peroxidation, delay aging and reduce the risk of chronic diseases. They can promote liver glycogen synthesis, improve mitochondrial function, and enhance energy metabolism efficiency. They can also promote the synthesis of collagen and elastin, and enhance the skin barrier function.

[0012] Fish peptide: is a small molecule active substance extracted from fish protein, mainly containing polypeptide, amino acid and trace element. Its core functions include promoting cell repair, enhancing immunity, regulating metabolic function, and also has antioxidant and anti-inflammatory properties, and is often used for nutritional supplementation or auxiliary improvement of skin and joint health. The small molecule peptides and amino acids in fish peptide can be directly absorbed by the human body, providing energy and synthesis materials for cells. It can activate metabolic enzyme activity, help protein and fat decomposition and utilization; the active peptides (such as collagen peptides and glutathione precursors) contained can neutralize free radicals and reduce oxidative stress damage to cells. Its antioxidant capacity can delay the loss of skin collagen, improve elasticity, and help reduce wrinkles.

[0013] The present application also provides a preparation method of the fish peptide complex, comprising the following steps: (1) drying and crushing avocado pulp to obtain avocado powder; mixing the avocado powder with ethanol, extracting, filtering, taking the filtrate, rotary evaporation to concentrate to 1 / 10 of the original volume, centrifuging, and collecting the oil layer to obtain avocado extract; (2) crushing rye seeds to obtain rye seed powder; mixing the rye seed powder with cellulase solution, ultrasonic extraction, 90~100℃ enzyme inactivation treatment for 5~10min, centrifuging to take the supernatant, rotary evaporation to concentrate to 1 / 8 of the original volume, and freeze-drying to obtain rye seed extract; (3) mixing the avocado extract obtained in step (1), the rye seed extract obtained in step (2), fish collagen peptide, elastin peptide, collagen tripeptide, tuna active peptide and fish peptide to obtain fish peptide complex.

[0014] Preferably, the drying temperature in step (1) is 35~45℃, and the drying time is 20~28h; the particle size of the avocado powder in step (1) is 80~120 mesh.

[0015] Preferably, the ratio of the avocado powder to ethanol in step (1) is 1g:1-3mL; the temperature of the extraction in step (1) is 50-70℃, and the extraction time is 6-18h; the centrifugal speed in step (1) is 8000-12000rpm, and the centrifugal time is 8-12min.

[0016] Preferably, the particle size of the rye seed powder in step (2) is 60-100 mesh.

[0017] Preferably, the ratio of the rye seed powder to cellulase solution in step (2) is 1g:4-6mL; the cellulase solution in step (2) is prepared by dissolving cellulase in a 40-60mmol / L sodium acetate solution, the mass concentration of cellulase in the cellulase solution is 10-20%, and the enzyme activity of the cellulase is 100000U.

[0018] Preferably, the ultrasonic extraction power in step (2) is 180-220W, the ultrasonic extraction temperature is 55-65℃, and the ultrasonic extraction time is 1.5-2.5h; the centrifugal speed in step (2) is 4000-6000rpm, and the centrifugal time is 8-12min; the freeze-drying temperature in step (2) is-40℃, the freeze-drying time is 24-72h, and the freeze-drying vacuum degree is 0.05Pa.

[0019] The application further provides use of the fish peptide compound in preparation of a product with antioxidant anti-wrinkle whitening functions.

[0020] Preferably, the product is a functional food or cosmetic.

[0021] Compared with the prior art, the application has the following advantages and technical effects: The present application provides a fish peptide compound with antioxidant anti-wrinkle whitening function, which is prepared by reasonably compounding fish-derived functional peptides and plant extracts, and each component plays a synergistic role. The prepared fish peptide compound has antioxidant, anti-wrinkle and whitening effects. Fish collagen peptide and rye seed extract containing arabinoxylan can play an antioxidant role by removing free radicals and inhibiting oxidative stress, and can enhance skin elasticity network structure, promote skin elasticity and moisture. The antioxidant function of rye seed extract is mainly derived from the free radical scavenging effect of phenolic substances such as ferulic acid, which can indirectly delay skin aging by reducing oxidative damage. The combination of the two can enhance the effect. The skin repair function of fish collagen peptide and the anti-inflammatory properties of rye seed extract can help improve uneven skin tone. Elastin peptide and avocado extract containing polyphenols can promote skin elasticity and antioxidant effects by removing free radicals and inhibiting oxidative stress, which can effectively protect cells from oxidative damage. Fish peptide and collagen tripeptide can play the roles of antioxidant, anti-inflammatory and promoting cell proliferation, can reduce melanin production by inhibiting tyrosinase activity, quickly penetrate into the dermis and be absorbed by the skin, promote the generation of collagen in the dermis, improve uneven skin tone by blocking the source of melanin and strengthening the skin water retention mechanism, and improve skin elasticity and moisture effect. The present application obtains a multi-effect fish peptide compound with antioxidant anti-wrinkle whitening function through the cooperation of each component. BRIEF DESCRIPTION OF DRAWINGS

[0022] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed in the embodiments. Obviously, the drawings in the following description only constitute some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained without creative labor.

[0023] Figure 1 The average back skin thickness of each group of mice after irradiation treatment is shown in the graph. Figure 2 The typical back skin state comparison chart of each group of mice after irradiation treatment is shown in the graph, wherein A is the model group of mice, B is the vitamin E group of mice, C is the example 1 group of mice, D is the comparative example 1 group of mice, E is the comparative example 2 group of mice, and F is the comparative example 3 group of mice. DETAILED DESCRIPTION

[0024] The detailed description of the various exemplary embodiments of the present application should not be considered as limiting the present application, but should be understood as a more detailed description of some aspects, characteristics and embodiments of the present application.

[0025] It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application. Additionally, for a range of values of, for example, the upper limit of the range and the lower limit of the range are each specifically disclosed. Each intermediate value of the range is either specifically disclosed or can be implicitly recognized regardless of whether it was

[0026] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. Although preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present application. All documents mentioned herein are incorporated by reference to disclose and describe in full the methods and / or materials which are described therein. In the case of conflict between the present specification and any document incorporated by reference, the present specification controls.

[0027] Many modifications and variations of the present application described in the specification are possible without departing from the scope or spirit of the application. Other embodiments of the application will be apparent to those skilled in the art from consideration of the specification and practice of the application disclosed herein. The specification and examples are illustrative only.

[0028] As used herein, the terms "comprises", "comprising", "includes", "including", "has", "having", "contains", "containing", or variations thereof, are intended to be open-ended terms that mean including, but not limited to.

[0029] The fish collagen peptide, elastin peptide, tuna active peptide, fish peptide and collagen tripeptide used in the present application are commercially available products.

[0030] Example 1 Fish collagen peptide 45 parts, elastin peptide 38 parts, collagen tripeptide 19.5 parts, tuna active peptide 15.5 parts, avocado extract 5.5 parts, fish peptide 3 parts, rye seed extract 2.8 parts.

[0031] (1) Avocado pulp was dried at 40°C for 24h, crushed, and the avocado powder with a particle size of 100 mesh was obtained. The avocado powder and ethanol were mixed at 1g:2mL, and extracted at 60°C for 12h. The filtrate was obtained by filtration, concentrated to 1 / 10 of the original volume by rotary evaporation at 200rpm and 78°C, and the oil layer was collected by centrifugation at 10000rpm for 10min, which was the avocado extract.

[0032] (2) The rye seeds were crushed to obtain rye seed powder with a particle size of 80 mesh. The rye seed powder was mixed with a cellulase solution (sodium acetate solution with a cellulase mass concentration of 15%, the concentration of the sodium acetate solution was 50 mmol / L, and the enzyme activity of the cellulase was 100000 U) at a ratio of 1 g:5 mL. Ultrasonic extraction was performed at 200 W and 60°C for 2 h. Enzyme inactivation treatment was performed at 95°C for 5 min. Centrifugation was performed at 5000 rpm for 10 min to obtain the supernatant. Rotary evaporation was performed at 100 rpm and 55°C to concentrate the supernatant to 1 / 8 of the original volume. Freeze-drying was performed at -40°C and 0.05 Pa for 48 h to obtain the rye seed extract.

[0033] (3) The avocado extract, the rye seed extract, the fish collagen peptide, the elastin peptide, the collagen tripeptide, the tuna active peptide, and the fish peptinn were mixed to obtain the fish peptide complex.

[0034] Example 2 The fish collagen peptide was 40 parts, the elastin peptide was 36 parts, the collagen tripeptide was 18 parts, the tuna active peptide was 14 parts, the avocado extract was 5 parts, the fish peptinn was 2 parts, and the rye seed extract was 2.6 parts.

[0035] (1) The avocado pulp was dried at 35°C for 28 h, crushed, and obtained avocado powder with a particle size of 80 mesh. The avocado powder was mixed with ethanol at a ratio of 1 g:1 mL. Extraction was performed at 50°C for 18 h. Filtration was performed. The filtrate was obtained. Rotary evaporation was performed at 200 rpm and 78°C to concentrate the filtrate to 1 / 10 of the original volume. Centrifugation was performed at 8000 rpm for 12 min. The oil layer was collected to obtain the avocado extract.

[0036] (2) The rye seeds were crushed to obtain rye seed powder with a particle size of 60 mesh. The rye seed powder was mixed with a cellulase solution (sodium acetate solution with a cellulase mass concentration of 10%, the concentration of the sodium acetate solution was 40 mmol / L, and the enzyme activity of the cellulase was 100000 U) at a ratio of 1 g:4 mL. Ultrasonic extraction was performed at 180 W and 55°C for 2.5 h. Enzyme inactivation treatment was performed at 90°C for 10 min. Centrifugation was performed at 4000 rpm for 12 min to obtain the supernatant. Rotary evaporation was performed at 100 rpm and 55°C to concentrate the supernatant to 1 / 8 of the original volume. Freeze-drying was performed at -40°C and 0.05 Pa for 24 h to obtain the rye seed extract.

[0037] (3) The avocado extract, the rye seed extract, the fish collagen peptide, the elastin peptide, the collagen tripeptide, the tuna active peptide, and the fish peptinn were mixed to obtain the fish peptide complex.

[0038] Example 3 The fish collagen peptide was 50 parts, the elastin peptide was 40 parts, the collagen tripeptide was 21 parts, the tuna active peptide was 17 parts, the avocado extract was 6 parts, the fish peptinn was 4 parts, and the rye seed extract was 3 parts.

[0039] (1) Avocado pulp was dried at 45℃ for 20h, and then pulverized to obtain an avocado powder with a particle size of 120 mesh. The avocado powder was mixed with ethanol at a ratio of 1g:3mL, and then extracted at 70℃ for 6h. The filtrate was obtained by filtration, and then concentrated to 1 / 10 of the original volume by rotary evaporation at 200rpm and 78℃. The oil layer was collected by centrifugation at 12000rpm for 8min, and then the avocado extract was obtained.

[0040] (2) The rye seeds were pulverized to obtain a rye seed powder with a particle size of 100 mesh. The rye seed powder was mixed with a cellulase solution (sodium acetate solution with a cellulase mass concentration of 20%, a sodium acetate solution concentration of 60mmol / L, and a cellulase enzyme activity of 100000U) at a ratio of 1g:6mL, and then ultrasonically extracted at 220W and 65℃ for 1.5h. The enzyme was inactivated by treatment at 100℃ for 5min. The supernatant was obtained by centrifugation at 6000rpm for 8min. The supernatant was concentrated to 1 / 8 of the original volume by rotary evaporation at 100rpm and 55℃. The rye seed extract was obtained by freeze-drying at -40℃ and 0.05Pa for 72h.

[0041] (3) The avocado extract, the rye seed extract, the fish collagen peptide, the elastin peptide, the collagen tripeptide, the tuna active peptide and the fish peptinn were mixed to obtain the fish peptide complex.

[0042] Comparative Example 1 The fish collagen peptide was 47.8 parts, the elastin peptide was 38 parts, the collagen tripeptide was 19.5 parts, the tuna active peptide was 15.5 parts, the avocado extract was 5.5 parts, and the fish peptinn was 3 parts.

[0043] (1) Avocado pulp was dried at 40℃ for 24h, and then pulverized to obtain an avocado powder with a particle size of 100 mesh. The avocado powder was mixed with ethanol at a ratio of 1g:2mL, and then extracted at 60℃ for 12h. The filtrate was obtained by filtration, and then concentrated to 1 / 10 of the original volume by rotary evaporation at 200rpm and 78℃. The oil layer was collected by centrifugation at 10000rpm for 10min, and then the avocado extract was obtained.

[0044] (2) The avocado extract, the fish collagen peptide, the elastin peptide, the collagen tripeptide, the tuna active peptide and the fish peptinn were mixed to obtain the fish peptide complex.

[0045] Comparative Example 2 The fish collagen peptide was 45 parts, the elastin peptide was 38 parts, the collagen tripeptide was 19.5 parts, the tuna active peptide was 15.5 parts, the avocado water extract was 5.5 parts, the fish peptinn was 3 parts, and the rye seed extract was 2.8 parts.

[0046] (1) Avocado pulp was dried at 40℃ for 24h, and then pulverized to obtain an avocado powder with a particle size of 100 mesh. The avocado powder was mixed with water at a ratio of 1g:2mL, and then extracted at 90℃ for 8h. The oil layer was collected, and then the avocado water extract was obtained.

[0047] (2) The rye seeds were crushed to obtain rye seed powder with a particle size of 80 mesh. The rye seed powder was mixed with a cellulase solution (sodium acetate solution with a cellulase mass concentration of 15%, a sodium acetate solution concentration of 50 mmol / L, and an enzyme activity of cellulase of 100000 U) at a ratio of 1 g:5 mL, and extracted by ultrasonic at 200 W and 60°C for 2 h. Enzyme inactivation treatment was performed at 95°C for 5 min. Centrifugation was performed at 5000 rpm for 10 min to obtain a supernatant. Concentration was performed by rotary evaporation at 100 rpm and 55°C to 1 / 8 of the original volume. Freeze-drying was performed at -40°C and 0.05 Pa for 48 h to obtain the rye seed extract.

[0048] (3) The avocado water extract, the rye seed extract, the fish collagen peptide, the elastin peptide, the collagen tripeptide, the tuna active peptide, and the fish peptide were mixed to obtain the fish peptide complex.

[0049] Comparative Example 3 The fish collagen peptide was 45 parts, the elastin peptide was 38 parts, the collagen tripeptide was 22.5 parts, the tuna active peptide was 15.5 parts, the avocado extract was 5.5 parts, and the rye seed extract was 2.8 parts.

[0050] (1) The avocado pulp was dried at 40°C for 24 h, crushed, and obtained avocado powder with a particle size of 100 mesh. The avocado powder was mixed with ethanol at a ratio of 1 g:2 mL, and extracted at 60°C for 12 h. Filtration was performed, and the filtrate was obtained. Concentration was performed by rotary evaporation at 200 rpm and 78°C to 1 / 10 of the original volume. Centrifugation was performed at 10000 rpm for 10 min, and the oil layer was collected to obtain the avocado extract.

[0051] (2) The rye seeds were crushed to obtain rye seed powder with a particle size of 80 mesh. The rye seed powder was mixed with a cellulase solution (sodium acetate solution with a cellulase mass concentration of 15%, a sodium acetate solution concentration of 50 mmol / L, and an enzyme activity of cellulase of 100000 U) at a ratio of 1 g:5 mL, and extracted by ultrasonic at 200 W and 60°C for 2 h. Enzyme inactivation treatment was performed at 95°C for 5 min. Centrifugation was performed at 5000 rpm for 10 min to obtain a supernatant. Concentration was performed by rotary evaporation at 100 rpm and 55°C to 1 / 8 of the original volume. Freeze-drying was performed at -40°C and 0.05 Pa for 48 h to obtain the rye seed extract.

[0052] (3) The avocado extract, the rye seed extract, the fish collagen peptide, the elastin peptide, the collagen tripeptide, and the tuna active peptide were mixed to obtain the fish peptide complex.

[0053] Experimental Example 1 The total superoxide dismutase (T-SOD) assay kit, the glutathione peroxidase (GSH-Px) activity assay kit, and the malondialdehyde (MDA) kit were all purchased from Nanjing Jiancheng Biological Engineering Institute.

[0054] 60 Kunming male mice, weighing 30-34 g, were adaptively fed for 4 days, and then randomly divided into 6 groups, 10 mice in each group. The blank control group was given 0.2 mL of physiological saline per mouse per day by gavage; the 5 mg / mL Vc group was a positive control group, and 0.2 mL of the prepared fish peptide compound solution per mouse per day was given by gavage; the Example 1 group was given 0.2 mL of the prepared fish peptide compound solution per mouse per day by gavage, and the dosage of the fish peptide compound was calculated as 10 mg / kg / d; the Comparative Example 1 group was given 0.2 mL of the prepared fish peptide compound solution per mouse per day by gavage, and the dosage of the fish peptide compound was calculated as 10 mg / kg / d; the Comparative Example 2 group was given 0.2 mL of the prepared fish peptide compound solution per mouse per day by gavage, and the dosage of the fish peptide compound was calculated as 10 mg / kg / d; and the Comparative Example 3 group was given 0.2 mL of the prepared fish peptide compound solution per mouse per day by gavage, and the dosage of the fish peptide compound was calculated as 10 mg / kg / d. The mice were continuously given by gavage for 30 days, and were allowed to freely eat and drink. After the gavage was completed, the mice were fasted for 12 h, and blood was taken from the eyeball, anticoagulated with heparin, centrifuged at 3,000 r / min for 15 min at 4 ℃ to prepare serum; the liver was cut, rapidly and thoroughly ground in an ice bath with physiological saline to prepare 10% liver homogenate, and centrifuged to take the liver homogenate supernatant for determination of relevant biochemical indexes.

[0055] The liver homogenate supernatant was diluted with physiological saline, and the concentration of liver protein was determined by the Coomassie brilliant blue method. The determination of superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-PX) activity and malondialdehyde (MDA) content was performed according to the kit. The average values of the mice in each group were determined, and the change trend of the biochemical indexes of the mice was observed.

[0056] Table 1 Determination results of SOD activity in serum and liver of mice in each group

[0057] As shown in Table 1, the fish peptide compound prepared in Example 1 can improve the activity of SOD in serum and liver, and after the compatibility of the fish collagen peptide and the extract of the black wheat seed in Comparative Example 1 is destroyed, the effect of the fish peptide compound is obviously affected, and the improvement of the SOD activity is not promoted.

[0058] Table 2 Determination results of GSH-PX activity in serum and liver of mice in each group

[0059] As shown in Table 2, the fish peptide compound prepared in Example 1 can improve the activity of GSH-PX in serum and liver, and especially can improve the activity of GSH-PX in liver, and after the compatibility of the fish collagen peptide and the extract of the black wheat seed in Comparative Example 1 is destroyed, the improvement of the GSH-PX activity is not promoted.

[0060] Table 3 Determination results of serum and liver MDA concentration of mice in each group

[0061] As shown in Table 3, the fish peptide complex prepared in Example 1 can reduce the MDA concentration in serum and liver, and especially can reduce the MDA concentration in serum. After destroying the compatibility of fish collagen peptide and the extract of rye seeds in the comparative example 1, the effect on MDA concentration is not obvious.

[0062] Experimental Example 2 SPF female mice with a body weight of 18-22 g were randomly divided into 6 groups, 10 mice in each group. The model control group (MC) was shaved and not given any drug, and was subjected to UV irradiation together with other groups. The vitamin E group (VE) was shaved, and 0.1 mL of VE cream preparation was uniformly applied to the back, and was subjected to UV irradiation after 1 h of treatment. The Example 1 group was shaved, and 0.1 mL of the fish peptide complex prepared in Example 1 with a concentration of 1 g / mL was uniformly applied to the back, and was subjected to UV irradiation after 1 h of treatment. The comparative example 1 group was shaved, and 0.1 mL of the fish peptide complex prepared in comparative example 1 with a concentration of 1 g / mL was uniformly applied to the back, and was subjected to UV irradiation after 1 h of treatment. The comparative example 2 group was shaved, and 0.1 mL of the fish peptide complex prepared in comparative example 2 with a concentration of 1 g / mL was uniformly applied to the back, and was subjected to UV irradiation after 1 h of treatment. The comparative example 3 group was shaved, and 0.1 mL of the fish peptide complex prepared in comparative example 3 with a concentration of 1 g / mL was uniformly applied to the back, and was subjected to UV irradiation after 1 h of treatment.

[0063] The ultraviolet lamp tube was fixed on the top of the carton, and UVA and UVB were irradiated separately. The lamp tube was preheated for 15 min before irradiation, and the irradiation intensity was measured respectively. The required irradiation time was converted according to the required irradiation dose per day. The minimum erythema dose (MED) of UVB was 80 mJ / cm 2 , and the UVB irradiation dose per day was set to 1 times MED for 1-3 days, 2 times MED for 4-6 days, 3 times MED for 7-14 days, and 4 times MED for 15-42 days. The cumulative UVB irradiation dose was 9.12 J / cm 2 . The daily irradiation dose of UVA was 10 times that of UVB, and the cumulative UVA irradiation dose was 91.2 J / cm 2 .

[0064] After the irradiation treatment was completed, the back skin was lifted, and the skin thickness of the same part of the mouse back was measured with a vernier caliper, which was the thickness of the mouse back skin.

[0065] As Figure 1The figure shows the average thickness of the skin on the back of mice in each group. The skin of the mice in the model control group was significantly thickened. The skin thickness of the mice in the comparative example 2 group was similar to that of the model group. The skin thickness of the mice in the example 1 group was similar to that of the mice in the vitamin E group. This indicates that the fish peptide complex prepared in Example 1 of this invention has a protective effect on the skin surface when applied, and can delay its damage and aging process. The fish peptide complex in the comparative example 2 group did not have a significant skin protective effect because it disrupted the compatibility between elastin peptide and avocado extract.

[0066] like Figure 2 China A Figure 2 B, Figure 2 C, Figure 2 D, Figure 2 China E and Figure 2 As shown in Figure F, this is a comparison of typical skin appearance of mice in each group after irradiation. The skin of the mice in the model control group is obviously dry, red, swollen, and damaged. The skin of the mice in the comparative group 3 is obviously dry and damaged to a certain extent. The skin of the mice in the Example 1 group and the vitamin E group showed basically no significant changes. This indicates that the fish peptide complex prepared in Example 1 of this invention has a moisturizing effect when applied to the skin surface. After ultraviolet irradiation, it can ensure that the skin does not lose moisture and maintains elasticity and smoothness.

[0067] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A fish peptide complex with antioxidant, anti-wrinkle, and whitening functions, characterized in that, Includes the following components by weight: Fish collagen peptides 40-50 parts, elastin peptides 36-40 parts, collagen tripeptides 18-21 parts, tuna active peptides 14-17 parts, avocado extract 5-6 parts, fish peptides 2-4 parts, rye seed extract 2.6-3.0 parts.

2. The fish peptide complex according to claim 1, characterized in that, It includes the following components by weight: 45 parts fish collagen peptide, 38 parts elastin peptide, 19.5 parts collagen tripeptide, 15.5 parts tuna active peptide, 5.5 parts avocado extract, 3 parts fish peptide, and 2.8 parts rye seed extract.

3. The method for preparing the fish peptide complex as described in claim 1 or 2, characterized in that, Includes the following steps: (1) Dry the avocado pulp, crush it to obtain avocado powder; mix the avocado powder with ethanol, extract it, filter it, take the filtrate, concentrate it to 1 / 10 of the original volume by rotary evaporation, centrifuge it, and collect the oil layer to obtain avocado extract. (2) Rye seeds are crushed to obtain rye seed powder; the rye seed powder is mixed with cellulase solution, ultrasonically extracted, treated with enzyme inactivation at 90~100℃ for 5~10 min, the supernatant is collected by centrifugation, concentrated to 1 / 8 of the original volume by rotary evaporation, and freeze-dried to obtain rye seed extract. (3) The avocado extract obtained in step (1), the rye seed extract obtained in step (2), fish collagen peptide, elastin peptide, collagen tripeptide, tuna active peptide and fish peptide are mixed to obtain the fish peptide complex.

4. The preparation method according to claim 3, characterized in that, The drying temperature in step (1) is 35~45℃ and the drying time is 20~28h; the particle size of the avocado powder in step (1) is 80~120 mesh.

5. The preparation method according to claim 3, characterized in that, The ratio of avocado powder to ethanol in step (1) is 1g: 1~3mL; the extraction temperature in step (1) is 50~70℃, and the extraction time is 6~18h; the centrifugation speed in step (1) is 8000~12000rpm, and the centrifugation time is 8~12min.

6. The preparation method according to claim 3, characterized in that, The rye seed powder mentioned in step (2) has a particle size of 60~100 mesh.

7. The preparation method according to claim 3, characterized in that, The ratio of rye seed powder to cellulase solution in step (2) is 1g: 4~6mL; the cellulase solution in step (2) is prepared by dissolving cellulase in sodium acetate solution with a concentration of 40~60mmol / L, the mass concentration of cellulase in the cellulase solution is 10~20%, and the enzyme activity of the cellulase is 100000U.

8. The preparation method according to claim 3, characterized in that, The ultrasonic extraction power in step (2) is 180~220W, the ultrasonic extraction temperature is 55~65℃, and the ultrasonic extraction time is 1.5~2.5h; the centrifugation speed in step (2) is 4000~6000rpm, and the centrifugation time is 8~12min; the freeze-drying temperature in step (2) is -40℃, the freeze-drying time is 24~72h, and the freeze-drying vacuum degree is 0.05Pa.

9. The use of the fish peptide complex as described in claim 1 or 2 in the preparation of products with antioxidant, anti-wrinkle, and whitening functions.

10. The application according to claim 9, characterized in that, The product is a functional food or cosmetic.

Images