Wrinkle-removing, moisturizing and skin-care composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of skin care products, and particularly relates to a wrinkle-removing moisturizing skin care composition as well as a preparation method and application thereof. The composition is prepared from the following raw materials in percentage by mass: 0.25 to 0.3 percent of dipeptide diaminobutyrylbenzyl amide diacetate, 0.5 to 0.8 percent of oligopeptide-6, 10 to 20 percent of leontopodium alpinum callus extract, 60 to 80 percent of glycerol and the balance of water. In the culture process of the leontopodium alpinum callus, nomilin, cinnamaldehyde and other components are innovatively added into an induced differentiation culture medium. Test results show that the content of the total flavonoids in the leontopodium alpinum callus can be effectively increased through the synergistic effect of the nomilin and the cinnamaldehyde, and the content of the total flavonoids in the leontopodium alpinum callus extract reaches 26.7% or above. The content of total flavonoids in the leontopodium alpinum callus extract is increased, so that the moisturizing and wrinkle-removing effects of the skin care product are remarkably improved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of skin care products, and specifically relates to a wrinkle-removing and moisturizing skin care composition as well as a preparation method and application thereof. BACKGROUND

[0002] In the context of exploring skin aging and its coping strategies, especially in the face of increasingly serious environmental pollution and ultraviolet radiation-induced skin problems, wrinkle removal and moisturization have become important needs in the skin care market. Skin care products with plant extracts as the core component have attracted much attention due to their traceable origin, safety and greenness, comprehensive functionality, wide availability, antioxidant and soothing sensitive skin, and effective free radical scavenging and blood circulation promotion.

[0003] Leontopodium alpinum Colm. ex Cass., also known as edelweiss, is a cold plant, and its extract contains rich minerals, can clear heat and cool blood, and has the effects of soothing and calming the skin, nourishing and protecting the skin, and resisting skin aging. Callus is a local part of a plant body that forms enlarged and dedifferentiated parenchyma cells on the surface of a wound after being stimulated by trauma. Chinese patent CN115137665A, a composition with long-term moisturizing and soothing effects and its application, discloses an edelweiss callus extract, which has excellent antioxidant properties and moisturizing capacity, can stimulate the expression of barrier-related genes, which has good protective capacity for skin under the influence of ultraviolet pollution and free radicals, and can resist aging caused by free radicals and inflammation. Chinese patent CN112870142A, an efficient moisturizing essence cream and its preparation method, discloses that the edelweiss callus extract can slow down the degradation of elastin, can maintain the elasticity of the skin, and keep the skin smooth. Chinese patent CN110693807A, a dermal essence and its preparation method, discloses that the edelweiss callus extract can bring a significant visible lifting effect to the skin, and has the effects of smoothing wrinkles and tightening the skin; meanwhile, its specific high antioxidant property can repair and maintain mitochondrial activity and improve its contraction ability, and can also promote the synthesis of collagen, so that the skin obtains sufficient collagen, the skin cells become plump, the skin becomes moist, delicate and smooth, and effectively resists aging.

[0004] The preparation method of the existing high mountain fireweed callus extract has shown certain antioxidant and moisturizing ability, but there is still room for improvement in the content of active ingredients and skin care effect. Chinese patent CN114392215A discloses a composition containing plant callus extract and its application, which involves the preparation method of high mountain fireweed callus extract. Although the above-mentioned patent can extract active ingredients with antioxidant and moisturizing functions, it still has problems such as low extraction efficiency and insufficient content of active ingredients, especially in the content of total flavonoids, which is difficult to meet the demand of high-end skin care products for high-efficiency skin care ingredients. As an important antioxidant, total flavonoids can effectively scavenge free radicals and inhibit the skin aging process, and are the key ingredients to improve the anti-wrinkle and moisturizing effect of skin care products.

[0005] Therefore, it is necessary to improve the culture process and extraction process of high mountain fireweed callus to significantly enhance the synthesis and accumulation of total flavonoids, thereby promoting its application in the field of skin care products. SUMMARY

[0006] The first object of the present application is to provide a wrinkle-removing and moisturizing skin care composition with excellent wrinkle-removing and moisturizing effects.

[0007] The second object of the present application is to provide a preparation method of a wrinkle-removing and moisturizing skin care composition, which is simple and easy to operate.

[0008] The third object of the present application is to provide an application of a wrinkle-removing and moisturizing skin care composition, which has a broad prospect.

[0009] To achieve the above objects, the technical scheme adopted by the present application is as follows: A wrinkle-removing and moisturizing skin care composition, comprising the following raw materials in mass percentage: dipeptide diaminobutyryl benzyl amide diacetate 0.25-0.3%, oligopeptide-6 0.5-0.8%, high mountain fireweed callus extract 10-20%, glycerol 60-80%, and the balance being water; The preparation method of the high mountain fireweed callus extract is as follows: (1) The sterilized high mountain fireweed seeds are inoculated into a sterile MS medium for culture to obtain sterile seedlings; (2) The stem segments of the sterile seedlings obtained in step (1) are used as explants, which are inoculated on a sterilized induction and differentiation medium for induction and culture of callus to obtain callus; the induction and differentiation medium comprises an MS medium and an inducer; the inducer comprises the following components: 2,4-D, NAA, cinnamyl aldehyde and nomilin; (3) The callus obtained in step (2) is collected, washed and dried, and then subjected to reflux extraction, and the extract is collected, filtered, concentrated and dried to obtain the high mountain fireweed callus extract.

[0010] Cinnamaldehyde and nomilin, as natural plant ingredients, exhibit a variety of biological activities in plant physiology and pharmacology research. Cinnamaldehyde, mainly present in plants such as cinnamon, has antibacterial, anti-inflammatory and cell metabolism promoting effects; and nomilin is known for its strong antioxidant and anti-inflammatory properties. Adding these two ingredients to the culture medium can not only promote the growth and differentiation of Ledum palustre callus, but also enhance the content of total flavonoids therein.

[0011] Further, the concentration of the inducer in the induction differentiation medium in step (2) is 2, 4-D 1-3 mg / L, NAA 0.1-0.3 mg / L, cinnamaldehyde 0.5-0.8 mg / L, and nomilin 0.2-0.5 mg / L, based on the final concentration of the induction differentiation medium.

[0012] Further, the temperature of the induction culture in step (2) is 18-22℃, the light intensity is 500-800lx, and the time is 15-20d.

[0013] Further, the temperature of the culture in step (1) is 18-22℃, the light intensity is 500-800lx, and the time is 10-15d.

[0014] Further, the solvent used for the reflux extraction in step (3) is an ethanol solution with a concentration of 70-80% (v / v), and the number of times of reflux extraction is 2-4 times.

[0015] The preparation method of the wrinkle-removing and moisturizing skin care composition comprises the following steps: According to the mass percentage content, the raw materials are mixed uniformly to obtain the composition.

[0016] The wrinkle-removing and moisturizing skin care composition is used in the preparation of skin care products.

[0017] Further, the mass percentage content of the wrinkle-removing and moisturizing skin care composition in the skin care product is 1-5%, based on the total mass of the skin care product.

[0018] Further, the skin care product further comprises the following raw materials with the following mass percentage contents: 1-3% squalane, 0.2-0.4% cetostearyl alcohol polyether-20, 0.5-1% behenyl alcohol, 0.05-0.1% p-hydroxyacetophenone, and the balance is water.

[0019] The beneficial technical effects of the present application are: (1) The application adds nomilin, cinnamyl aldehyde and the like to the induction differentiation culture medium in the culture process of the high mountain firebrush callus. The test results show that the synergistic effect of nomilin and cinnamyl aldehyde can effectively improve the content of total flavones in the high mountain firebrush callus, and the content of total flavones in the high mountain firebrush callus extract of the application is more than 26.7%.

[0020] (2) The skin care product of the application is mild and non-irritating to the skin, safe to use, and has excellent moisturizing effect and wrinkle removal effect. The test results show that after using the skin care product of the application for 28 days, the skin hydration water content change rate is more than 59.05%, the absolute value of wrinkle area change rate is more than 40.33%, and the absolute value of wrinkle depth change rate is more than 31.95%. DETAILED DESCRIPTION

[0021] The following is a further detailed description of the application in conjunction with specific preferred embodiments, which cannot be deemed to limit the specific implementation of the application to these descriptions. For ordinary skilled persons in the technical field to which the application belongs, without departing from the concept of the application, a number of simple deductions or substitutions can be made, which should be regarded as falling within the protection scope of the application. The specific conditions not mentioned in the examples are carried out according to the conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments used, such as those not specifically mentioned, are conventional products obtained through market channels.

[0022] (I) Preparation Example Preparation Example 1 A high mountain firebrush callus extract is prepared as follows: (1) The high mountain firebrush seeds are first washed with water, then sterilized with 75% ethanol for 40s, and then sterilized with 4% sodium hypochlorite solution for 8min, and the seeds are washed with sterile water to obtain the sterilized high mountain firebrush seeds. The sterilized high mountain firebrush seeds are inoculated into sterile MS culture medium, and cultured at a temperature of 20℃ and a light intensity of 600 lx for 12d. After the culture is completed, sterile seedlings are obtained.

[0023] (2) The stems of the sterile seedlings in step (1) are taken and cut into 1cm stem segments, which are used as explants and inoculated on the sterilized induction differentiation culture medium, and cultured at a temperature of 20℃ and a light intensity of 600 lx for 18d to obtain callus. The induction differentiation culture medium consists of MS culture medium and an induction agent. The concentration of the induction agent in the induction differentiation culture medium is 2mg / L of 2,4-D, 0.2mg / L of NAA, 0.7mg / L of cinnamyl aldehyde and 0.3mg / L of nomilin, based on the final concentration of the induction differentiation culture medium.

[0024] (3) Collect the callus obtained in step (2), and after washing and drying, extract the callus with 75% ethanol by refluxing for 3 times, collect the extract, and then filter, concentrate and dry the extract to obtain the high mountain firebrush callus extract.

[0025] Preparation Example 2 A high mountain firebrush callus extract is prepared according to the following process: (1) First, wash the high mountain firebrush seeds with water, then sterilize the seeds with 75% ethanol for 40s, and then sterilize the seeds with 4% sodium hypochlorite solution for 8min, and then wash the seeds with sterile water to obtain the sterilized high mountain firebrush seeds. The sterilized high mountain firebrush seeds are inoculated into sterile MS medium, and cultured at a temperature of 22℃ and an illumination intensity of 500lx for 10d. After the culture, sterile seedlings are obtained.

[0026] (2) Take the stem segments of the sterile seedlings obtained in step (1) and divide the stem segments into 1cm stem segments, and inoculate the stem segments as explants into the sterilized induction and differentiation medium, and induce and culture the stem segments at a temperature of 22℃ and an illumination intensity of 500lx for 15d to obtain callus. The induction and differentiation medium comprises MS medium and an induction agent. The concentration of the induction agent in the induction and differentiation medium is 2,4-D 3mg / L, NAA 0.3mg / L, cinnamyl aldehyde 0.8mg / L, and nomilin 0.5mg / L, based on the final concentration of the induction and differentiation medium.

[0027] (3) Collect the callus obtained in step (2), and after washing and drying, extract the callus with 70% ethanol by refluxing for 4 times, collect the extract, and then filter, concentrate and dry the extract to obtain the high mountain firebrush callus extract.

[0028] Preparation Example 3 A high mountain firebrush callus extract is prepared according to the following process: (1) First, wash the high mountain firebrush seeds with water, then sterilize the seeds with 75% ethanol for 40s, and then sterilize the seeds with 4% sodium hypochlorite solution for 8min, and then wash the seeds with sterile water to obtain the sterilized high mountain firebrush seeds. The sterilized high mountain firebrush seeds are inoculated into sterile MS medium, and cultured at a temperature of 18℃ and an illumination intensity of 800lx for 15d. After the culture, sterile seedlings are obtained.

[0029] (2) Take the stem segments of the sterile seedlings obtained in step (1) and divide the stem segments into 1cm stem segments, and inoculate the stem segments as explants into the sterilized induction and differentiation medium, and induce and culture the stem segments at a temperature of 18℃ and an illumination intensity of 800lx for 20d to obtain callus. The above induction differentiation medium is composed of MS medium and an inducing agent; the concentration of the inducing agent in the induction differentiation medium is 2,4-D 1 mg / L, NAA 0.1 mg / L, cinnamaldehyde 0.5 mg / L, nomilin 0.2 mg / L, in terms of the final concentration of the induction differentiation medium.

[0030] (3) The callus obtained in step (2) is collected, washed and dried, and then extracted with 80% ethanol for 2 times, and the extract is collected. The extract is filtered, concentrated and dried to obtain the extract of the callus of Leontopodium hircinum.

[0031] Preparation Example 4 Preparation Example 4 is different from Preparation Example 1 in that: The concentration of the inducing agent in the induction differentiation medium is 2,4-D 2 mg / L, NAA 0.2 mg / L, nomilin 0.3 mg / L, and the rest is the same as Preparation Example 1.

[0032] Preparation Example 5 Preparation Example 5 is different from Preparation Example 1 in that: The concentration of the inducing agent in the induction differentiation medium is 2,4-D 2 mg / L, NAA 0.2 mg / L, nomilin 1 mg / L, and the rest is the same as Preparation Example 1.

[0033] Preparation Example 6 Preparation Example 6 is different from Preparation Example 1 in that: The concentration of the inducing agent in the induction differentiation medium is 2,4-D 2 mg / L, NAA 0.2 mg / L, cinnamaldehyde 0.7 mg / L, and the rest is the same as Preparation Example 1.

[0034] Preparation Example 7 Preparation Example 7 is different from Preparation Example 1 in that: The concentration of the inducing agent in the induction differentiation medium is 2,4-D 2 mg / L, NAA 0.2 mg / L, cinnamaldehyde 1 mg / L, and the rest is the same as Preparation Example 1.

[0035] Preparation Example 8 Preparation Example 8 is different from Preparation Example 1 in that: The concentration of the inducing agent in the induction differentiation medium is 2,4-D 2 mg / L, NAA 0.2 mg / L, and the rest is the same as Preparation Example 1.

[0036] (II) Examples Example 1 A wrinkle-removing and moisturizing skin care composition, which is composed of the following raw materials in mass percentage: dipeptide diaminobutyryl benzyl amide diacetate 0.27%, oligopeptide-6 0.6%, high mountain firebrush callus extract prepared in Preparation Example 1 12%, glycerin 75%, and the balance of water.

[0037] The preparation method of the above-mentioned wrinkle-removing and moisturizing skin care composition is as follows: According to the above-mentioned mass percentage, the raw materials are mixed uniformly to obtain the composition.

[0038] Example 2 A wrinkle-removing and moisturizing skin care composition, which is composed of the following raw materials in mass percentage: dipeptide diaminobutyryl benzyl amide diacetate 0.25%, oligopeptide-6 0.5%, high mountain firebrush callus extract prepared in Preparation Example 2 10%, glycerin 60%, and the balance of water.

[0039] The preparation method of the above-mentioned wrinkle-removing and moisturizing skin care composition is as follows: According to the above-mentioned mass percentage, the raw materials are mixed uniformly to obtain the composition.

[0040] Example 3 A wrinkle-removing and moisturizing skin care composition, which is composed of the following raw materials in mass percentage: dipeptide diaminobutyryl benzyl amide diacetate 0.3%, oligopeptide-6 0.8%, high mountain firebrush callus extract prepared in Preparation Example 3 20%, glycerin 80%, and the balance of water.

[0041] The preparation method of the above-mentioned wrinkle-removing and moisturizing skin care composition is as follows: According to the above-mentioned mass percentage, the raw materials are mixed uniformly to obtain the composition.

[0042] (Three) Comparative Example Comparative Example 1 A skin care composition, which is composed of the following raw materials in mass percentage: dipeptide diaminobutyryl benzyl amide diacetate 0.27%, oligopeptide-6 0.6%, high mountain firebrush callus extract prepared in Preparation Example 4 12%, glycerin 75%, and the balance of water, and the rest is the same as Example 1.

[0043] Comparative Example 2 A skin care composition, which is composed of the following raw materials in mass percentage: dipeptide diaminobutyryl benzyl amide diacetate 0.27%, oligopeptide-6 0.6%, high mountain firebrush callus extract prepared in Preparation Example 5 12%, glycerin 75%, and the balance of water, and the rest is the same as Example 1.

[0044] Comparative Example 3 A skin care composition consisting of the following ingredients in the amounts indicated: dipeptide diaminobutyryl benzylamide diacetate 0.27%, oligopeptide-6 0.6%, high mountain fire fireweed callus extract prepared in Preparation Example 6 12%, glycerin 75%, the balance being water, the remainder being as in Example 1.

[0045] Comparative Example 4 A skin care composition consisting of the following ingredients in the amounts indicated: dipeptide diaminobutyryl benzylamide diacetate 0.27%, oligopeptide-6 0.6%, high mountain fire fireweed callus extract prepared in Preparation Example 7 12%, glycerin 75%, the balance being water, the remainder being as in Example 1.

[0046] Comparative Example 5 A skin care composition consisting of the following ingredients in the amounts indicated: dipeptide diaminobutyryl benzylamide diacetate 0.27%, oligopeptide-6 0.6%, high mountain fire fireweed callus extract prepared in Preparation Example 8 12%, glycerin 75%, the balance being water, the remainder being as in Example 1.

[0047] (IV) Test Examples Test Example 1 The total flavonoid content of the high mountain fire fireweed callus extract prepared in Preparation Examples 1-8 was determined according to the following procedure: The high mountain fire fireweed callus extract prepared in Preparation Examples 1-8 was ground and sieved through a 40 mesh screen to obtain a high mountain fire fireweed callus extract powder; 0.0500 g of the above powder was weighed into a 20 mL centrifuge tube, 10 mL of 75% ethanol was added to the centrifuge tube, mixed well, and then ultrasonically treated for 25 min; after ultrasonic treatment, the centrifuge was set to 4000 rpm and centrifuged for 15 min to obtain a supernatant; 3 mL of the above supernatant was taken and placed in a 25 mL volumetric flask, 0.5 mL of 5% sodium nitrite solution was added to the volumetric flask, shaken well, and allowed to stand for 5 min; 0.5 mL of 10% aluminum nitrate solution was added to the volumetric flask, shaken well, and allowed to stand for 5 min; 5 mL of sodium hydroxide solution was added to the volumetric flask, diluted to volume with purified water, shaken well, and allowed to stand for 18 min; a blank control group was also set up, which did not add high mountain fire fireweed callus extract, but only the reagents used.

[0048] The absorbance value of the supernatant of each group was measured at a wavelength of 500 nm using a spectrophotometer, and rutin was used as a control to prepare a standard curve of absorbance versus concentration. The total flavonoid content of each group was calculated by the standard curve, and the results are shown in Table 1.

[0049] Table 1 As shown in Table 1, the content of total flavonoids in the Leontice sibiricum callus extract prepared in Preparation Examples 1-3 is higher, reaching more than 26.7%.

[0050] Compared with Preparation Examples 1-3, the content of total flavonoids in the Leontice sibiricum callus extract prepared in Preparation Examples 4-8 is reduced to different degrees, and the content of total flavonoids in the Leontice sibiricum callus extract prepared in Preparation Example 8 is the lowest, only 6.9%. In the process of preparing the Leontice sibiricum callus, the induction and differentiation medium of Preparation Example 4 is not added with cinnamyl aldehyde, the induction and differentiation medium of Preparation Example 5 is not added with cinnamyl aldehyde but the use amount of nomilin is increased, the induction and differentiation medium of Preparation Example 6 is not added with nomilin, the induction and differentiation medium of Preparation Example 7 is not added with nomilin but the use amount of cinnamyl aldehyde is increased, and the induction and differentiation medium of Preparation Example 8 is not added with nomilin and cinnamyl aldehyde. As known from the above, the change of components in the induction and differentiation medium affects the content of total flavonoids in the Leontice sibiricum callus, and the synergistic effect of nomilin and cinnamyl aldehyde can effectively increase the content of total flavonoids in the Leontice sibiricum callus.

[0051] The effects of the anti-wrinkle and moisturizing skin care compositions added with the examples 1-3 and comparative examples 1-5 of the present application are described below.

[0052] A skin care product, which is composed of the following raw materials in mass percentage: 2% squalane, 0.3% cetostearyl alcohol polyether-20, 0.6% behenyl alcohol, 0.08% p-hydroxyacetophenone, 3% each of the above anti-wrinkle and moisturizing skin care compositions, and the balance of water.

[0053] Test Example 2 The safety of the skin care product added with the anti-wrinkle and moisturizing skin care compositions of examples 1-3 and comparative examples 1-5 is detected, and the specific process is as follows: Subjects: 80 subjects aged 18-60 years old are selected and randomly divided into 8 groups, and the male to female ratio in each group is 1:1.

[0054] Detection method: 0.02 g of the skin care product added with the anti-wrinkle and moisturizing skin care compositions of examples 1-3 and comparative examples 1-5 is taken and placed in a patch tester, the patch tester is covered on the flexor of the forearm of the subject, and the skin surface is uniformly attached by pressing with the palm for 24 h. After 30 min of removing the patch tester, the skin reaction is observed after the indentation disappears. If the result is negative, the skin reaction is observed again at 24 h and 48 h after the patch test. The results of the patch test are judged according to the skin reaction grading standard in Table 2, and the results are shown in Table 3.

[0055] Table 2 Table 3 As shown in Table 3, the skin care product provided by the application is mild and non-irritating to the skin, and is safe to use.

[0056] Test Example 3 The wrinkle-removing and moisturizing effects of the skin care products obtained by adding the wrinkle-removing and moisturizing skin care compositions of Examples 1-3 and Comparative Examples 1-5 were detected according to QB / T 4256-2011 "Guidelines for Moisturizing Effect Evaluation of Cosmetics" and T / ZHCA 006-20119 "Anti-wrinkle Effect Test Method for Cosmetics", and the specific process was as follows: Subjects: 80 healthy female subjects aged 18-60 years were selected and randomly divided into 8 groups.

[0057] Test environment: the temperature was 22°C and the humidity was 50%, and direct sunlight was avoided.

[0058] Detection method: all subjects entered the test environment for 30 minutes before starting the test. The subjects applied the skin care products added with the wrinkle-removing and moisturizing skin care compositions of Examples 1-3 and Comparative Examples 1-5 to the face twice a day in the morning and evening, and the wrinkle area, wrinkle depth, and skin water content were detected before use and 28 days after use of the skin care products. Each group of data was the average value of the test results of the group.

[0059] Among them, the skin hydration content change rate (%) = (skin hydration content after 28 days of using the skin care product - skin hydration content before use) / skin hydration content before use x 100%, and the results are shown in Table 4; The wrinkle area change rate (%) = (wrinkle area after 28 days of using the skin care product - wrinkle area before use) / wrinkle area before use x 100%, and the results are shown in Table 5; The wrinkle depth change rate (%) = (wrinkle depth after 28 days of using the skin care product - wrinkle depth before use) / wrinkle depth before use x 100%, and the results are shown in Table 6.

[0060] Table 4 Table 5 Table 6 The higher the value of the change rate of skin hydration content, the better the moisturizing effect of the skin care product. The higher the absolute value of the change rate of wrinkle area and the change rate of wrinkle depth, the better the wrinkle-removing effect of the skin care product. As can be seen from Tables 4-6, the skin care product of the present application has excellent moisturizing effect and wrinkle-removing effect. Specifically, after using the skin care product of the present application for 28 days, the change rate of skin hydration content is above 59.05%, the absolute value of the change rate of wrinkle area is above 40.33%, and the absolute value of the change rate of wrinkle depth is above 31.95%.

[0061] Compared with Examples 1-3, the moisturizing and wrinkle-removing effects of the skin care products of Comparative Examples 1-5 are reduced to different degrees. However, the skin care products of Comparative Examples 1-5 each contain the high mountain firebush callus extract prepared in Preparation Example 4-8. In combination with the total flavonoid content of the high mountain firebush callus extract, it can be seen that the increase of the total flavonoid content can promote the moisturizing and wrinkle-removing effects of the skin care product.

[0062] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application, and not to limit it. The basic principles and main features of the present application have been described above with specific embodiments, and some modifications or substitutions can be made on the basis of the present application, but these modifications or substitutions do not make the corresponding technical solutions deviate from the scope of the present application.

Claims

1. A wrinkle-reducing and moisturizing skincare composition, characterized in that, The product consists of the following raw materials in the indicated weight percentages: dipeptide diaminobutyryl benzylamide diacetate 0.25-0.3%, oligopeptide-6 0.5-0.8%, edelweiss callus extract 10-20%, glycerin 60-80%, and the remainder being water; The preparation method of the Edelweiss callus extract is as follows: (1) Sterilized seeds of Edelweiss were inoculated into sterile MS medium for culture to obtain sterile seedlings; (2) Take the stem segment of the sterile seedling in step (1) as the explant, inoculate it on the sterilized induction differentiation medium for callus induction culture, and obtain callus; the induction differentiation medium includes MS medium and inducing agent; the inducing agent includes the following components: 2,4-D, NAA, cinnamaldehyde, nomiline; (3) Collect the callus tissue obtained in step (2), wash and dry it, then reflux extract it, collect the extract, filter, concentrate and dry the extract to obtain the Edelweiss callus tissue extract.

2. The anti-wrinkle and moisturizing skincare composition as described in claim 1, characterized in that, Based on the final concentration of the induction differentiation medium, the concentration of the inducing agent in step (2) in the induction differentiation medium is 2,4-D 1-3 mg / L, NAA 0.1-0.3 mg / L, cinnamaldehyde 0.5-0.8 mg / L, and nomiline 0.2-0.5 mg / L.

3. The anti-wrinkle and moisturizing skincare composition as described in claim 1, characterized in that, The induction culture in step (2) is carried out at a temperature of 18-22℃, a light intensity of 500-800 lx, and a time of 15-20 days.

4. The anti-wrinkle and moisturizing skincare composition as described in claim 1, characterized in that, The culture temperature in step (1) is 18-22℃, the light intensity is 500-800 lx, and the time is 10-15 days.

5. The anti-wrinkle and moisturizing skincare composition as described in claim 1, characterized in that, The solvent used for reflux extraction in step (3) is an ethanol solution with a concentration of 70-80% (v / v), and the reflux extraction is performed 2-4 times.

6. The method for preparing the anti-wrinkle and moisturizing skincare composition according to any one of claims 1-5, characterized in that, Includes the following steps: Mix all raw materials evenly according to the stated mass percentage to obtain the final product.

7. The application of the anti-wrinkle and moisturizing skincare composition according to any one of claims 1-5, characterized in that, Applications in the preparation of skin care products.

8. The application of the anti-wrinkle and moisturizing skincare composition as described in claim 7, characterized in that, The anti-wrinkle and moisturizing skincare composition comprises 1-5% by weight of the total weight of the skincare products.

9. The application of the anti-wrinkle and moisturizing skincare composition as described in claim 7, characterized in that, The skincare product also includes the following ingredients in weight percentage: 1-3% squalane, 0.2-0.4% cetearyl alcohol polyether-20, 0.5-1% behenol, 0.05-0.1% p-hydroxyacetophenone, with the balance being water.

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