Skin repairing and anti-aging composition capable of improving cell viability and application of skin repairing and anti-aging composition in cosmetics

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a skin repairing and anti-aging composition capable of improving cell viability and application of the skin repairing and anti-aging composition in cosmetics. The skin repairing and anti-aging composition is prepared from the following components in parts by mass: 0.5 to 5 parts of crithmum maritimum cell-glowing essence, 0.5 to 2.5 parts of coastal eryngii repairing cell essence, 0.7 to 1.8 parts of vitamin granule and 0.3 to 0.5 part of artemisia sphaerocephala krasch extract. The composition provided by the invention can simultaneously promote epidermis repair, dermis anti-aging, true epidermis connecting band repair and increase the ATP synthase content, has obvious repair and anti-aging effects, and has a wide market prospect.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a skin repair and anti-aging composition that enhances cell activity and its application in cosmetics. Background Technology

[0002] As the largest organ in the human body, the skin's outermost layer, the epidermis, not only protects the body from external environmental damage but also participates in vital functions such as sensation and moisture retention. A damaged epidermal barrier can lead to increased moisture loss, dry skin, and heightened sensitivity. While many existing skincare products claim to repair the epidermis, their actual effectiveness often varies from person to person and lacks a long-term, stable guarantee of results.

[0003] The dermis and the dermo-epidermal junction are rich in collagen and elastin, which are crucial for maintaining skin firmness and elasticity. With age or exposure to factors such as UV damage, the dermis gradually loses these supporting structures, leading to wrinkles and sagging skin. While many anti-aging skincare products are available, they typically take a long time to show results and their effects are limited, making it difficult to significantly reverse existing signs of aging.

[0004] ATP (adenosine triphosphate) is the primary energy currency within cells and is crucial for cellular life processes. The ATP level in skin cells directly affects the rate of cellular metabolism and repair capabilities. Enhancing cellular energy levels by increasing the content of ATP synthase in the skin is considered a new direction for improving skin condition.

[0005] With the increasing demand for beauty and personal care, the market is flooded with cosmetics claiming multiple benefits. However, very few products can truly achieve the four benefits simultaneously: promoting epidermal repair, dermal anti-aging, dermal-epidermal junction (DEJ) repair, and increasing ATP synthase levels (energy skincare). Most existing products tend to focus on one or two aspects, failing to fully meet consumers' needs for highly effective skincare. Therefore, developing a composition that can achieve all three effects simultaneously is particularly important. Summary of the Invention

[0006] The present invention aims to provide an innovative cosmetic composition that can simultaneously promote epidermal repair, resist dermal aging, improve the epidermal-dermal junction (DEJ), and increase ATP synthase content. This composition not only represents a major advancement in skin care technology but also opens up new avenues for future research.

[0007] To achieve the above objectives, the present invention provides the following technical solution: A skin repair and anti-aging composition for improving cell activity, comprising the following components in parts by weight: 0.5-5 parts of sea fennel revitalizing cell extract, 0.5-2.5 parts of sea fennel repairing cell extract, 0.7-1.8 parts of vitamin, and 0.3-0.5 parts of Artemisia annua extract.

[0008] This invention improves the anti-aging and repairing effects of the skin repair and anti-aging composition by compounding sea fennel revitalizing cell essence, coastal erythrorhizon repairing cell essence, vitamin, and artemisia annua extract.

[0009] The mass ratio of sea fennel revitalizing cell essence, sea fennel repairing cell essence, and vitamin A is 1:1:1.

[0010] Preferably, the sea fennel cell-revitalizing extract comprises the following components by weight percentage: 26-32% sea fennel callus culture filtrate, 25-35% butylene glycol, and the balance being water.

[0011] Preferably, the sea celery repair cell essence comprises the following components by weight percentage: 23-34% sea celery callus culture filtrate, 35-45% butylene glycol, and the balance being water.

[0012] Preferably, the vitamin comprises the following components by mass percentage: 1.0-4.0% ribose, 1.0-8.0% artichoke extract, 1.0-8.0% trisodium fructose diphosphate, 3.0-8.0% glycerol, 0.5-2.0% 1,2-hexanediol, 0.1-0.5% p-hydroxyacetophenone, with the balance being water.

[0013] Preferably, the sea fennel cell-revitalizing extract comprises the following components by weight percentage: 30% sea fennel callus culture filtrate, 30% butylene glycol, and the remainder being water.

[0014] Preferably, the sea celery repair cell essence comprises the following components by weight percentage: 30% sea celery callus culture filtrate, 40% butylene glycol, and the remainder is water.

[0015] Preferably, the vitamin comprises the following components by mass percentage: 2.3% ribose, 5.4% Artemia extract, 6.2% trisodium fructose diphosphate, 7.0% glycerol, 1.5% 1,2-hexanediol, 0.2% p-hydroxyacetophenone, and the balance being water.

[0016] The quality of sea fennel revitalizing cell extract accounts for 30-40% of the sum of the quality of sea fennel revitalizing cell extract, sea fennel repairing cell extract, and vitamin C.

[0017] When the mass of the sea fennel revitalizing cell extract accounts for 30-40% of the sum of the masses of the sea fennel revitalizing cell extract, the sea celery repairing cell extract, and the vitamin A, the skin repair and anti-aging composition of the present invention has a better repair effect.

[0018] The preparation method of the skin repair and anti-aging composition that enhances cell activity includes the following steps: mixing sea fennel revitalizing cell essence, sea celery repairing cell essence, vesin and artemisia annua extract, and stirring evenly to obtain the product.

[0019] A second aspect of the present invention provides the application of the aforementioned skin repair and anti-aging composition for enhancing cell activity in the preparation of cosmetics.

[0020] Preferably, the amount of the skin repair and anti-aging composition added to the cosmetic is 1-5 wt%.

[0021] Preferably, the cosmetic is one of the following: skin care water, lotion, serum, cream, facial mask liquid, and gel.

[0022] Preferably, the cosmetic is a lotion.

[0023] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows: 1. This invention provides a skin repair and anti-aging composition that enhances cell activity. It selects sea fennel revitalizing cell essence, coastal eryngium repairing cell essence, vitamin and artemisia extract, and through scientific formulation, it has low irritation and excellent repair effect. At the same time, it has better anti-aging effect and can simultaneously achieve epidermal repair, dermal anti-aging, dermal-epidermal junction repair and increase ATP synthase content.

[0024] 2. When the mass of the sea fennel revitalizing cell essence accounts for 30-40% of the sum of the masses of the sea fennel revitalizing cell essence, the sea fennel repairing cell essence, and the vitamin A, the skin repair and anti-aging composition of the present invention has a better repair effect.

[0025] 3. This invention combines sea fennel cell-revitalizing essence, sea celery cell-repairing essence, and vitamin with artemisia extract, resulting in a better anti-aging effect. Attached Figure Description

[0026] Figure 1 The diagram shows the cell scratch test of the composition in Example 1. The left image represents 0h, and the right image represents 24h.

[0027] Figure 2 The diagram shows the cell scratch test results of the composition in Comparative Example 1. The left image represents 0h, and the right image represents 24h.

[0028] Figure 3The diagram shows the cell scratch test results of the composition in Comparative Example 2. The left image represents 0h, and the right image represents 24h.

[0029] Figure 4 The diagram shows the cell scratch test results of the composition in Comparative Example 3. The left image represents 0h, and the right image represents 24h.

[0030] Figure 5 The diagram shows the cell scratch test results of the composition in Comparative Example 4. The left image represents 0h, and the right image represents 24h.

[0031] Figure 6 The diagram shows the cell scratch test results of the composition in Comparative Example 5. The left image represents 0h, and the right image represents 24h.

[0032] Figure 7 The diagram shows the cell scratch test results of the composition in Comparative Example 6. The left image represents 0h, and the right image represents 24h.

[0033] Figure 8 This is a schematic diagram of the blank control for the cell scratch assay; the left image represents 0h, and the right image represents 24h.

[0034] Figure 9 A schematic diagram of the repair rate in a scratch test. Figure 10 A schematic diagram of ATPase content per unit protein. Figure 11 A diagram illustrating the content of type I collagen. Figure 12 A schematic diagram of type III collagen content. Figure 13 This is a schematic diagram showing the content of type IV collagen. Detailed Implementation

[0035] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0036] All raw materials used in the following embodiments of the present invention are commercially available products.

[0037] Sea Fennel Cell Revitalizing Essence, manufactured by Chongqing Ansaibo New Material Technology Co., Ltd., contains the following components by weight percentage: 30% sea fennel callus culture filtrate, 30% butylene glycol, and the remainder is water.

[0038] Seaweed Cervi Cell Repairing Essence, Manufacturer: Chongqing Ansaibo New Material Technology Co., Ltd., contains the following components by weight percentage: 30% Seaweed Cervi Callus Culture Filtrate, 40% Butylene Glycol, and the balance is water.

[0039] MitoVitalin, manufactured by Huibai Biotechnology (Guangzhou) Co., Ltd., trade name: MitoVitalin253, contains the following components by weight percentage: 2.3% ribose, 5.4% Artemia extract, 6.2% trisodium fructose diphosphate, 7.0% glycerol, 1.5% 1,2-hexanediol, 0.2% p-hydroxyacetophenone, balance being water.

[0040] Artemisia abrata extract, manufacturer: SILAB SA, France; Blue algae extract, Baoji Kosmike Cosmetic Raw Material Development Co., Ltd.

[0041] Haematococcus pluvialis extract, Fufeng Sinote Biotechnology Co., Ltd.

[0042] Extract of *Leptochloa crus-galli*, Baoji Kosmike Cosmetic Raw Material Development Co., Ltd.

[0043] Green tea extract, Nanjing Tongying Biotechnology Co., Ltd.

[0044] Rosemary extract, Baoji Fangcheng Biotechnology Development Co., Ltd.

[0045] Butanediol is 1,3-butanediol.

[0046] Sea fennel revitalizing cell extract, sea celery repairing cell extract, and vitamin were added at the mass percentages shown in Table 1. Routine cell scratch tests, ATPase content tests per unit protein, and in vitro fibroblast type I / III / IV collagen content assays were performed. Results are shown below. Figure 1-13 .

[0047] Table 1. Percentage of mass used in the testing system (%)

[0048] pass Figure 1-13 It was found that the combination of sea fennel revitalizing cell essence, sea celery repairing cell essence, and vitamin had better repair and anti-aging effects. To further improve the anti-aging and repairing effects of the skin repair composition and its gentleness on sensitive skin, Artemisia aquilina extract was added to the formula of sea fennel revitalizing cell essence, sea celery repairing cell essence, and vitamin for testing.

[0049] Example 1 This embodiment provides a skin repair and anti-aging composition for improving cell activity, comprising the following components in parts by weight: 1 part sea fennel revitalizing cell essence, 1 part sea fennel repairing cell essence, 1 part vesin, and 0.4 parts Artemisia annua extract.

[0050] The preparation method of the skin repair and anti-aging composition that enhances cell activity includes the following steps: mixing sea fennel revitalizing cell essence, sea celery repairing cell essence, vesin and artemisia annua extract, and stirring evenly to obtain the product.

[0051] Example 2 This embodiment provides a skin repair and anti-aging composition for improving cell activity, comprising the following components in parts by weight: 2.1 parts of sea fennel revitalizing cell essence, 2.4 parts of sea fennel repairing cell essence, 0.94 parts of vitamin, and 0.3 parts of Artemisia annua extract.

[0052] The preparation method of the skin repair and anti-aging composition that enhances cell activity includes the following steps: mixing sea fennel revitalizing cell essence, sea celery repairing cell essence, vesin and artemisia annua extract, and stirring evenly to obtain the product.

[0053] Example 3 This embodiment provides a skin repair and anti-aging composition for improving cell activity, comprising the following components in parts by weight: 0.89 parts of sea fennel revitalizing cell essence, 0.57 parts of sea fennel repairing cell essence, 1.5 parts of vitamin, and 0.5 parts of Artemisia annua extract.

[0054] The preparation method of the skin repair and anti-aging composition that enhances cell activity includes the following steps: mixing sea fennel revitalizing cell essence, sea celery repairing cell essence, vesin and artemisia annua extract, and stirring evenly to obtain the product.

[0055] Comparative Example 1 The difference between this comparative example and Example 1 is that the Artemisia annua extract was replaced with cyanobacteria extract.

[0056] Comparative Example 2 The difference between this comparative example and Example 1 is that the Artemisia annua extract was replaced with Haematococcus pluvialis extract.

[0057] Comparative Example 3 The difference between this comparative example and Example 1 is that the Artemisia annua extract was replaced with Laminaria japonica extract.

[0058] Comparative Example 4 The difference between this comparative example and Example 1 is that the Artemisia annua extract was replaced with green tea extract.

[0059] Comparative Example 5 The difference between this comparative example and Example 1 is that the Artemisia annua extract was replaced with rosemary extract.

[0060] Comparative Example 6 The difference between this comparative example and Example 1 is that the total amount of sea fennel revitalizing cell essence, sea fennel repairing cell essence, and vitamin C remains the same, but the ratio is changed.

[0061] A skin repair and anti-aging composition for improving cell activity, comprising the following components in parts by weight: 0.5 parts sea fennel revitalizing cell extract, 1.2 parts sea fennel repairing cell extract, 1.3 parts vitamin, and 0.4 parts Artemisia annua extract.

[0062] Comparative Example 7 The difference between this comparative example and Example 1 is that the total amount of sea fennel revitalizing cell essence, sea fennel repairing cell essence, and vitamin C remains the same, but the ratio is changed.

[0063] A skin repair and anti-aging composition for improving cell activity, comprising the following components in parts by weight: 1.5 parts sea fennel revitalizing cell extract, 0.7 parts sea fennel repairing cell extract, 0.8 parts vitamin, and 0.4 parts Artemisia annua extract.

[0064] Comparative Example 8 The difference between this comparative example and Example 1 is that the amount of Artemisia annua extract used is different.

[0065] A skin repair and anti-aging composition for improving cell activity, comprising the following components in parts by weight: 1 part sea fennel revitalizing cell extract, 1 part sea fennel repairing cell extract, 1 part vesin, and 0.1 part Artemisia annua extract.

[0066] Comparative Example 9 The difference between this comparative example and Example 1 is that the amount of Artemisia annua extract used is different.

[0067] A skin repair and anti-aging composition for improving cell activity, comprising the following components in parts by weight: 1 part sea fennel revitalizing cell extract, 1 part sea fennel repairing cell extract, 1 part vesin, and 0.6 parts Artemisia aquilina extract.

[0068] Comparative Example 10 The difference between this comparative example and Example 1 is that the total amount of *Erycibe obtusifolia* cell repairing essence and *Artemisia aquilina* extract remains the same, but the ratio is changed. One part of *Erycibe obtusifolia* cell repairing essence and 0.4 parts of *Artemisia aquilina* extract are replaced with 0.7 parts of *Erycibe obtusifolia* cell repairing essence and 0.7 parts of *Artemisia aquilina* extract.

[0069] Performance testing The performance of the skin repair and anti-aging compositions prepared in Examples 1-3 and Comparative Examples 1-10 was tested.

[0070] 1. The hydroxyl radical scavenging rate was tested according to the method in CN118453476B. The results are shown in Table 1.

[0071] Table 1. Results of Hydroxyl Radical Scavenging Rate Test

[0072] The results show that the skin repair and anti-aging compositions of Examples 1-3 have excellent anti-aging effects, which are superior to the anti-aging effects of the anti-aging compositions in the prior art (CN118453476B).

[0073] When the *Artemisia aquilinum* extract was replaced with other components in Comparative Examples 1-5, the anti-aging effect decreased. This indicates that only the *Artemisia aquilinum* extract can achieve a better anti-aging effect in the system.

[0074] In Comparative Example 6, the anti-aging effect was significantly reduced due to changes in the ratio of sea fennel revitalizing cell extract, coastal erythrorhizon repairing cell extract, and vitamin C. This indicates that the three components have a synergistic effect on anti-aging.

[0075] In Comparative Example 8, the amount of Artemisia annua extract used was too small, resulting in a decreased anti-aging effect.

[0076] 2. Sensitive skin adaptability test Testing the suitability of skin repair and anti-aging compositions for sensitive skin.

[0077] The test sample was prepared by adding 5 wt% of the skin repair and anti-aging composition to glycerin.

[0078] Testing Method: 180 volunteers aged 20-40 years with capsaicin-positive sensitive skin were selected. The volunteers were randomly divided into 6 groups, with the sample applied to half of their face. After 28 days, according to the adverse skin reaction requirements described in the "Cosmetic Safety Technical Specifications - 2015 Edition" (Table 2), dermatologists scored the overall skin condition of the volunteers. Results are shown in Table 3.

[0079] Table 2 Requirements for Adverse Skin Reactions

[0080] Table 3 Test Results

[0081] The results showed that replacing the Artemisia arundinacea extract with other extracts in Comparative Examples 1, 2, and 5 increased the irritation to sensitive skin. In Comparative Example 8, the amount of Artemisia arundinacea extract was too low, resulting in mild irritation to sensitive skin. In Comparative Example 6, the altered ratio of sea fennel revitalizing cell extract, Erycibe obtusifolia repairing cell extract, and vitamin also caused mild irritation to sensitive skin.

[0082] 3. Ninety volunteers aged 20-40 years with capsaicin-positive sensitive skin were selected and randomly divided into 9 groups. A single-layer filter paper with a diameter of 0.8 cm was placed on the nasolabial fold of the volunteers. 50 μL of 10 wt% lactic acid aqueous solution was dropped onto the filter paper. The subjects were asked about their stinging sensation at 0.5 min and 2 min, and scored on a 4-point scale (0 points for no stinging, 1 point for mild stinging, 2 points for moderate stinging, and 3 points for severe stinging). The experiment was terminated if the subject scored 3 points at any time during the experiment.

[0083] The test sample was prepared as follows: The skin repair and anti-aging composition was added to Pechoin glycerin at a concentration of 2 wt%. Usage: Volunteers applied the sample to their entire face; volunteers in the control group used glycerin as the sample. Volunteers applied approximately 1 mL of the product twice daily, morning and evening, as instructed, for two consecutive weeks. The test procedure was repeated after two weeks of use.

[0084] Record the lactic acid stinging scores of volunteers before and after 2 weeks of use, and calculate the improvement rate of lactic acid stinging. Lactic acid stinging improvement rate (%) = (average lactic acid stinging score before use - average lactic acid stinging score after 2 weeks of use) / average lactic acid stinging score before use × 100%. Average lactic acid stinging score = average stinging score at 0.5 minutes + average stinging score at 2 minutes. A higher lactic acid stinging improvement rate indicates a better repair effect of the sample.

[0085] The results are shown in Table 4.

[0086] Table 4 Repair Results

[0087] The results show that the skin repair and anti-aging compositions of Examples 1-3 have excellent repair effects.

[0088] When Artemisia arundinacea extract was replaced with other components in Comparative Examples 3 and 4, the repairing effect of the skin repair and anti-aging composition decreased.

[0089] Comparative Example 7 shows that the ratio of sea fennel revitalizing cell essence, sea celery repairing cell essence, and vitamin affects the repair effect of the skin repair and anti-aging composition.

[0090] In Comparative Example 9, the proportion of Artemisia annua extract was too high, which reduced the repair effect of the skin repair and anti-aging composition.

[0091] Comparative studies 3, 4, and 10 demonstrate that the sea celery cell repairing essence and Artemisia annua extract have a synergistic effect.

[0092] The above description represents the preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A skin repair and anti-aging composition for enhancing cell activity, characterized in that, It includes the following components in parts by weight: 0.5-5 parts sea fennel revitalizing cell extract, 0.5-2.5 parts sea fennel repairing cell extract, 0.7-1.8 parts vitamin A and 0.3-0.5 parts Artemisia annua extract.

2. The skin repair and anti-aging composition for improving cell activity according to claim 1, characterized in that, The sea fennel revitalizing cell essence comprises the following components by weight percentage: 26-32% sea fennel callus culture filtrate, 25-35% butylene glycol, and the balance being water.

3. The skin repair and anti-aging composition for improving cell activity according to claim 2, characterized in that, The sea celery repair cell essence comprises the following components by weight percentage: 23-34% sea celery callus culture filtrate, 35-45% butylene glycol, and the balance being water.

4. The skin repair and anti-aging composition for improving cell activity according to claim 3, characterized in that, The vitamin comprises the following components by weight percentage: 1.0-4.0% ribose, 1.0-8.0% Artemia extract, 1.0-8.0% trisodium fructose diphosphate, 3.0-8.0% glycerol, 0.5-2.0% 1,2-hexanediol, 0.1-0.5% p-hydroxyacetophenone, with the balance being water.

5. The skin repair and anti-aging composition for improving cell activity according to claim 1, characterized in that, The quality of sea fennel revitalizing cell extract accounts for 30-40% of the sum of the quality of sea fennel revitalizing cell extract, sea fennel repairing cell extract, and vitamin C.

6. The skin repair and anti-aging composition for improving cell activity according to claim 1, characterized in that, The mass ratio of sea fennel revitalizing cell essence, sea fennel repairing cell essence, and vitamin A is 1:1:

1.

7. The use of a skin repair and anti-aging composition for improving cell activity as described in any one of claims 1-6 in the preparation of cosmetics.

8. The application of the skin repair and anti-aging composition for improving cell activity according to claim 7 in the preparation of cosmetics, characterized in that, The amount of the skin repair and anti-aging composition added to the cosmetic is 1-5 wt%.

9. The application of the skin repair and anti-aging composition for improving cell activity according to claim 8 in the preparation of cosmetics, characterized in that, The cosmetic product is one of the following: skin care water, lotion, serum, cream, facial mask liquid, and gel.

10. The application of the skin repair and anti-aging composition for improving cell activity according to claim 9 in the preparation of cosmetics, characterized in that: The cosmetic product is an emulsion.

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