Strain tank with double sterilization methods

Abstract

The invention relates to the technical field of strain tanks, and discloses a strain tank with double sterilization methods, the strain tank comprises a tank body, a cover body, a stirring motor, a stirring rod and a discharge pipe, the bottom surface of the tank body is fixedly connected with a steam pipe, the upper surface of the cover body is provided with a fixing opening in a penetrating manner, and the inner wall of the fixing opening is connected with a telescopic assembly; the other end of the telescopic assembly penetrates into the tank body and is connected with an ultraviolet lamp, the stirring rod is sleeved with the ultraviolet lamp, the top face of the inner wall of the cover body is connected with an expansion-shrinkage assembly, one end of the expansion-shrinkage assembly communicates with the end, away from the ultraviolet lamp, of the telescopic assembly, and the steam pipe communicates with the interior of the tank body. According to the strain tank with the double sterilization methods, the high-temperature steam sterilization mode and the ultraviolet sterilization mode are combined, a complementary and synergistic sterilization system is formed, and high-temperature-resistant microorganisms and bacterial spores can be effectively killed; through a linkage mechanism of the expansion and contraction assembly and the telescopic assembly, the ultraviolet lamp can automatically ascend and descend in the sterilization process, and all-directional dead-corner-free sterilization in the tank body is achieved.

Description

Technical Field

[0001] This invention relates to the field of inoculum culture tank technology, specifically to an inoculum culture tank with dual sterilization methods. Background Technology

[0002] A microbial culture tank (also known as a seed tank or microbial culture tank) refers to a specialized container or equipment used in the microbial fermentation industry for culturing and expanding microbial strains.

[0003] The patent with publication number CN220724160U discloses a microbial fermentation tank, including a tank body, a tank cover, and a discharge chamber. The bottom of the discharge chamber is equipped with a discharge pipe, the top of the tank cover is connected to a feed pipe, and the bottom of the discharge chamber is equipped with a discharge mechanism. In this invention, after the culture medium is filtered through a filter cylinder, the culture medium is isolated on the outside of the filter cylinder. The culture medium enters the inside of the discharge pipe through the sieve holes inside the filter cylinder. The culture medium is discharged out through the discharge pipe. The nut on the threaded rod is unscrewed, and then the gasket is pulled out from the inside of the slot. At this time, the threaded rod and the baffle move downwards at the same time. Then, the baffle is moved out from the bottom of the discharge chamber along the inner wall of the slot, which facilitates the removal of the culture medium from the discharge chamber. This separates the culture medium and the culture medium for discharge, solving the problem in the prior art that when the culture medium is discharged out through the discharge pipe alone, it is easy for the culture medium containing microorganisms to be discharged out, resulting in low purity of the microorganisms in the culture medium.

[0004] However, the above-mentioned inoculum containers still have the following problems in actual use: In microbial fermentation processes, sterilization is crucial for preventing contamination by other microorganisms and ensuring the pure culture of target strains and smooth production. Currently, most inoculum tanks employ a single sterilization method, with moist heat sterilization (high-temperature steam sterilization, also known as continuous sterilization) being the most common. However, some culture media contain heat-sensitive components such as proteins, vitamins, and enzymes (e.g., vaccine serum, probiotic culture media), which are easily denatured and inactivated by temperatures above 121°C (e.g., immunoglobulins coagulate above 60°C). Simultaneously, bacterial spores (e.g., Bacillus subtilis spores) are extremely heat-resistant (requiring temperatures above 130°C for 30 minutes for inactivation), and conventional 121°C / 30-minute high-temperature sterilization is insufficient to completely kill them, easily leaving spore residues that can lead to subsequent contamination. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a culture tank with dual sterilization methods, employing two sterilization techniques to achieve more comprehensive and thorough sterilization.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a microbial culture tank with dual sterilization methods, comprising a tank body, a cover mounted on the tank body, a stirring motor mounted on the cover, a stirring rod mounted on the output shaft of the stirring motor and located inside the tank body, and a discharge pipe mounted at the bottom of the tank body. A steam pipe is fixedly connected to the bottom surface of the tank body. A fixed opening is provided through the upper surface of the cover. A telescopic component is connected to the inner wall of the fixed opening. The other end of the telescopic component extends into the tank body and is connected to an ultraviolet lamp. The ultraviolet lamp is sleeved outside the stirring rod. An expansion and contraction component is connected to the top surface of the inner wall of the cover. One end of the expansion and contraction component is connected to the end of the telescopic component away from the ultraviolet lamp. The steam pipe is connected to the inside of the tank body. A valve is provided at the end of the steam pipe located outside the tank body. A pressure sensor is fixedly connected to the top surface of the inner wall of the cover.

[0007] Furthermore, an installation cavity is provided on the top surface of the inner wall of the cover, and the expansion and contraction assembly is located inside the installation cavity.

[0008] Furthermore, the expansion and contraction assembly includes an airbag, a semi-circular plate, and a connecting tube. The semi-circular plate is located inside the mounting cavity. The bottom surface of the semi-circular plate is fixedly connected to the upper surface of the airbag. One end of the airbag is fixedly connected to one end of the connecting tube, and the other end of the connecting tube is connected to the telescopic assembly. The airbag and the connecting tube are in communication.

[0009] Furthermore, a number of installation ports are provided on one side of the installation cavity, and a number of installation blocks are fixedly connected to the side wall of the semi-circular plate. The installation blocks are located in the installation ports respectively, and the installation blocks are detachably connected to the cover by bolts. An opening is provided through the end of the airbag away from the semi-circular plate.

[0010] Furthermore, a support block is fixedly connected to the same side of both the semi-circular plate and the airbag. A displacement sensor is fixedly connected to the upper surface of the support block near the airbag, and a timing sensor is fixedly connected to the bottom surface of the support block near the semi-circular plate.

[0011] Furthermore, the telescopic assembly includes an outer tube, a connecting rod, an L-shaped tube, an inner tube, a T-shaped ring, an extension rod, and a piston. The outer wall of the outer tube is fixedly connected to the inner wall of the fixing port. Both the inner walls of the outer tube and the inner tube have air cavities. The lower end of the extension rod is fixedly connected to the outer wall of the ultraviolet lamp, and the upper end of the extension rod is connected to the lower end of the connecting rod. The upper end of the connecting rod passes through the air cavity of the inner tube and is fixedly connected to the piston. The piston is slidably connected to the air cavity of the inner tube. The upper end of the inner tube passes through the interior of the outer tube and is fixedly connected to the bottom surface of the T-shaped ring. The outer walls of the inner tube and the outer walls of the T-shaped ring are slidably connected to the air cavity of the outer tube. One side of the upper end of the outer tube is fixedly connected to one end of the L-shaped tube, and the other end of the L-shaped tube is fixedly connected to the end of the connecting tube away from the air bladder. The connecting tube, the L-shaped tube, and the interior of the air cavity are connected. The diameter of the outer wall of the piston is larger than the diameter of the inner wall of the T-shaped ring.

[0012] Furthermore, a connecting flange is provided between the upper end of the extension rod and the lower end of the connecting rod, and the extension rod and the connecting rod are detachably connected through the connecting flange.

[0013] Furthermore, a connection port is provided on one side of the fixed port, and a side tube is fixedly connected to the inner wall of the connection port. One side of the side tube is fixedly connected to the side wall of the upper end of the outer tube. A mating interface is provided on the bottom surface of the side tube, and both the connection tube and the L-shaped tube are located inside the mating interface.

[0014] Furthermore, a receiving tube is fixedly connected to the upper surface of the outer tube, and a receiving cavity is opened inside the receiving tube. A tension spring is fixedly connected to the top surface of the receiving cavity, and the other end of the tension spring passes through the air cavity and is fixedly connected to the upper surface of the piston.

[0015] Furthermore, the number of outer tubes is not limited to one, and the connection relationship between two adjacent outer tubes is the same as the connection relationship between the outer tube and the inner tube.

[0016] Compared with the prior art, the present invention has the following beneficial effects: 1. This inoculum tank with dual sterilization methods combines high-temperature steam sterilization and ultraviolet sterilization to form a complementary and synergistic sterilization system, which can effectively kill heat-resistant microorganisms and bacterial spores. 2. This type of inoculum container with dual sterilization methods uses a linkage mechanism between expansion and contraction components and telescopic components to allow the ultraviolet lamp to automatically rise and fall during the sterilization process, achieving all-round sterilization without dead angles inside the container. 3. This inoculum tank with dual sterilization methods integrates multiple sensors and feedback mechanisms. The pressure sensor monitors the pressure inside the tank in real time. When the set threshold is reached, the exhaust pipe is automatically opened to release steam, avoiding damage to the tank body caused by continuous high pressure. The displacement sensor monitors the degree of air bladder contraction, and the timing sensor keeps time synchronously. If the ultraviolet lamp does not move as expected after the pressure reaches the standard, it can promptly warn of air leakage or malfunction, ensuring stable sterilization conditions. 4. This type of inoculum container with dual sterilization methods features a UV lamp that can be detachably connected to an extension rod via a connecting flange, facilitating quick disassembly and repair in case of damage. In addition, the semi-circular plate is bolted to the cover via an mounting block, and the airbag can be removed entirely from the mounting cavity, making it convenient to replace aging airbags periodically. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall appearance of the present invention; Figure 2 This is a schematic diagram of the overall appearance of the invention from another perspective; Figure 3 This is a detailed connection diagram of the cover, telescopic component, and expansion / contraction component of the present invention; Figure 4 For the present invention Figure 3 Enlarged view of point A in the middle; Figure 5 This is a detailed connection diagram of the components of the present invention, including the cover, stirring motor, and stirring rod. Figure 6 For the present invention Figure 5 Enlarged view of point B in the middle; Figure 7 This is a detailed connection diagram of the telescopic component, expansion and contraction component, and ultraviolet lamp of the present invention; Figure 8 This is a detailed connection diagram of the expansion and contraction assembly, support block, and displacement sensor of the present invention; Figure 9 This is a cross-sectional schematic diagram of the telescopic component of the present invention; Figure 10 For the present invention Figure 9 Enlarged view of point C in the middle; Figure 11 For the present invention Figure 9 Enlarged diagram of point D in the middle.

[0018] In the diagram: 1. Tank body; 2. Cover; 3. Agitator motor; 4. Storage pipe; 5. Outer pipe; 6. Side pipe; 7. Steam pipe; 8. Agitator rod; 9. Discharge pipe; 10. Airbag; 11. Opening; 12. Connecting rod; 13. Ultraviolet lamp; 14. Connecting flange; 15. Mounting cavity; 16. Fixing port; 17. Connection port; 18. Mounting port; 19. Semi-circular plate; 20. Support block; 21. Displacement sensor; 22. Mounting block; 23. Connecting pipe; 24. Timing sensor; 25. Tension spring; 26. Air chamber; 27. Connecting interface; 28. L-shaped pipe; 29. ​​Inner pipe; 30. T-ring; 31. Pressure sensor; 32. Extension rod; 33. Storage cavity; 34. Piston. Detailed Implementation

[0019] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0020] Please see Figures 1-11A microbial culture tank with dual sterilization methods includes a tank body 1, a cover 2 mounted on the tank body 1, a stirring motor 3 mounted on the cover 2, a stirring rod 8 mounted on the output shaft of the stirring motor 3 and located inside the tank body 1, and a discharge pipe 9 mounted at the bottom of the tank body 1. A steam pipe 7 is fixedly connected to the bottom surface of the tank body 1. A fixing port 16 is opened through the upper surface of the cover 2. A telescopic component is connected to the inner wall of the fixing port 16. The other end of the telescopic component extends into the tank body 1 and is connected to an ultraviolet lamp 13. The ultraviolet lamp 13 is sleeved on the outside of the stirring rod 8. An expansion and contraction component is connected to the top surface of the inner wall of the cover 2. One end of the expansion and contraction component is connected to the end of the telescopic component away from the ultraviolet lamp 13. The steam pipe 7 is connected to the inside of the tank body 1. A valve is provided at the end of the steam pipe 7 located outside the tank body 1. A pressure sensor 31 is fixedly connected to the top surface of the inner wall of the cover 2.

[0021] It is important to note here that: 1. The tank body 1, the cover body 2, the stirring motor 3, the stirring rod 8, and the discharge pipe 9 are all mature technologies of existing inoculum tanks. Their connection relationship, position relationship and working principle will not be described in detail here. In this invention, only the disclosed patent structure (such as the tank body 1 and the cover body 2) is partially improved. Some structures, such as the stirring motor 3, the stirring rod 8 and the discharge pipe 9, are not modified in any way and retain their original functions and effects. 2. By setting pressure sensor 31, the internal pressure of tank 1 can be monitored in real time, and the exhaust pipe at the top of tank 1 can be opened in time after the pressure reaches the set standard, so that the steam accumulated inside tank 1 can be discharged as soon as possible, thereby reducing the additional damage to tank 1 caused by continuous high pressure.

[0022] As a preferred embodiment of the present invention, the top surface of the inner wall of the cover 2 is provided with an installation cavity 15, and the expansion and contraction assembly is located in the installation cavity 15.

[0023] More specifically, by providing the mounting cavity 15, installation space can be provided for the expansion and contraction assembly, while ensuring the connection stability of the expansion and contraction assembly.

[0024] As a preferred embodiment of the present invention, the expansion and contraction assembly includes an airbag 10, a semi-circular plate 19, and a connecting tube 23. The semi-circular plate 19 is located in the mounting cavity 15. The bottom surface of the semi-circular plate 19 is fixedly connected to the upper surface of the airbag 10. One end of the airbag 10 is fixedly connected to one end of the connecting tube 23. The other end of the connecting tube 23 is connected to the telescopic assembly. The airbag 10 and the connecting tube 23 are in communication.

[0025] More specifically, by setting up an expansion and contraction component, as steam is introduced into the tank 1, the inside of the tank 1 is sterilized at high temperature, and the air pressure inside the tank 1 increases, which in turn forces the expansion and contraction component to shrink, thereby controlling the extension of the telescopic component.

[0026] As a preferred embodiment of the present invention, a plurality of mounting ports 18 are provided on one side of the mounting cavity 15, and a plurality of mounting blocks 22 are fixedly connected to the side wall of the semi-circular plate 19. The plurality of mounting blocks 22 are respectively located in the plurality of mounting ports 18, and the mounting blocks 22 are detachably connected to the cover body 2 by bolts. An opening 11 is provided through the end of the airbag 10 away from the semi-circular plate 19.

[0027] More specifically, by setting the mounting port 18, mounting block 22 and opening 11, the expansion and contraction components can be removed from the cover 2 when the airbag 10 needs to be disassembled for inspection or replacement after a period of use.

[0028] As a preferred embodiment of the present invention, a support block 20 is fixedly connected to the same side of the semi-circular plate 19 and the airbag 10. A displacement sensor 21 is fixedly connected to the upper surface of the support block 20 near the airbag 10, and a timing sensor 24 is fixedly connected to the bottom surface of the support block 20 near the semi-circular plate 19.

[0029] More specifically, as the airbag 10 gradually shrinks, the telescopic component gradually extends. By setting support blocks 20 on both the semi-circular plate 19 and the airbag 10, and by setting displacement sensors 21 and timing sensors 24 on the two support blocks 20, the compression state of the airbag 10 can be monitored in real time by the displacement sensors 21 and timing sensors 24. When the displacement sensor 21 detects that the airbag is close to the top of the cover 2 and has reached a specified range, or when the pressure sensor 31 detects that the pressure has started to rise, the timing sensor 24 will start timing. If the displacement sensor 21 has not risen to the specified position within the set time, it means that there is air leakage inside the tank 1 or that the displacement sensor 21 has malfunctioned. Thus, the displacement sensor 21 can be used to achieve double protection. It should be noted that the timing sensor 24 can be connected to an external controller (such as a PLC controller) via a connecting cable, so that staff can be notified in a timely manner outside the tank 1 when an alarm is triggered during the timing process.

[0030] In a preferred embodiment of the present invention, the telescopic assembly includes an outer tube 5, a connecting rod 12, an L-shaped tube 28, an inner tube 29, a T-shaped ring 30, an extension rod 32, and a piston 34. The outer wall of the outer tube 5 is fixedly connected to the inner wall of the fixing port 16. Air cavities 26 are provided on the inner walls of both the outer tube 5 and the inner tube 29. The lower end of the extension rod 32 is fixedly connected to the outer wall of the ultraviolet lamp 13, and the upper end of the extension rod 32 is connected to the lower end of the connecting rod 12. The upper end of the connecting rod 12 extends into the air cavity 26 of the inner tube 29 and is fixedly connected to the piston 34. Piston 34 is slidably connected to the air chamber 26 of inner tube 29. The upper end of inner tube 29 extends into the interior of outer tube 5 and is fixedly connected to the bottom surface of T-ring 30. The outer wall of inner tube 29 and the outer wall of T-ring 30 are slidably connected to the air chamber 26 of outer tube 5. One side of the upper end of outer tube 5 is fixedly connected to one end of L-shaped tube 28. The other end of L-shaped tube 28 is fixedly connected to the end of connecting tube 23 away from airbag 10. Connecting tube 23, L-shaped tube 28 and air chamber 26 are connected. The diameter of the outer wall of piston 34 is larger than the diameter of the inner wall of T-ring 30.

[0031] More specifically, by setting up a telescopic component, the expansion and contraction components can shrink while steam is introduced into the tank 1, and the telescopic component can extend at this time. This allows the ultraviolet lamp 13 connected to the lower end of the extension rod 32 to descend, thereby enabling the ultraviolet lamp 13 to rise and fall inside the tank 1. This solves the problem of the conventional ultraviolet lamp 13 having an unchangeable position and resulting in a small disinfection range.

[0032] As a preferred embodiment of the present invention, a connecting flange 14 is provided between the upper end of the extension rod 32 and the lower end of the connecting rod 12, and the extension rod 32 and the connecting rod 12 are detachably connected through the connecting flange 14.

[0033] More specifically, by providing the connecting flange 14, the extension rod 32 and the ultraviolet lamp 13 can be disassembled and separated from the connecting rod 12 when the ultraviolet lamp 13 is damaged or during routine maintenance.

[0034] As a preferred embodiment of the present invention, a connection port 17 is provided on one side of the fixed port 16, and a side tube 6 is fixedly connected to the inner wall of the connection port 17. One side of the side tube 6 is fixedly connected to the side wall of the upper end of the outer tube 5. A mating interface 27 is provided on the bottom surface of the side tube 6, and the connecting tube 23 and the L-shaped tube 28 are both located in the mating interface 27.

[0035] More specifically, by setting the connection port 17 and the side pipe 6, it is possible to ensure that the gas inside the airbag 10 can be introduced into the telescopic assembly through the connection pipe 23 to push the piston 34 down, while preventing gas leakage inside the tank 1.

[0036] As a preferred embodiment of the present invention, a receiving tube 4 is fixedly connected to the upper surface of the outer tube 5, and a receiving cavity 33 is opened inside the receiving tube 4. A tension spring 25 is fixedly connected to the top surface of the receiving cavity 33, and the other end of the tension spring 25 passes through the air cavity 26 and is fixedly connected to the upper surface of the piston 34.

[0037] More specifically, by setting up a receiving tube 4 and a tension spring 25 inside the receiving tube 4, when the venting begins inside the tank 1, the tension spring 25 can pull the piston 34 and other components back to their original positions, thus waiting for the next use. At the same time, it can also prevent the ultraviolet lamp 13 from being located at the lower end of the tank 1, which would affect the subsequent culture of bacteria.

[0038] As a preferred embodiment of the present invention, the number of outer tubes 5 is not limited to one, and the connection relationship between two adjacent outer tubes 5 is the same as the connection relationship between the outer tube 5 and the inner tube 29.

[0039] More specifically, by setting multiple outer tubes 5 (such as...) Figure 3 , Figure 7 and Figure 9 As shown, this can reduce the length of a single outer tube 5.

[0040] In summary, such as Figures 1 to 11 As shown, when the sterilization begins, the inoculum tank with dual sterilization methods in this invention only needs to open the valve of the steam pipe 7 located outside the tank 1, and then high-temperature steam can enter the tank 1 to sterilize the tank 1. At the same time as the steam enters the tank 1, because the exhaust pipe of the tank 1 is closed, the steam will not overflow, and the pressure inside the tank 1 will gradually increase. As the internal pressure of the tank 1 gradually increases, the airbag 10 located on the side of the cover 2 near the tank 1 will gradually be compressed by the pressure. At the same time as the airbag 10 is compressed, the gas inside the airbag 10 will enter the L-shaped tube 28 through the connecting tube 23, and then enter the air chamber 26 of the outer tube 5 from the L-shaped tube 28. The gas entering the gas chamber 26 of the outer tube 5 will push the piston 34 down from above. The piston 34 will first move to the bottom of the inner tube 29 as it descends. Then the piston 34 will continue to descend, which will push the inner tube 29 down together. At this time, the connecting rod 12 and the extension rod 32 located below the piston 34 will descend together, which will bring the ultraviolet lamp 13 connected to the extension rod 32 down with it. With ultraviolet lamp 13 (such as Figure 9As shown, the ultraviolet lamp 13 is a ring lamp, and the outer shell of the ultraviolet lamp 13 is made of quartz glass tube. The heat resistance of the quartz glass tube is much higher than that of steam, so it will not be affected by steam. The ultraviolet lamp 13 can be lowered and gradually moved downward from the top of the tank 1, so that the inside of the tank 1 can be sterilized in another form during the movement. As steam gradually enters the tank 1, the pressure sensor 31 located at the top of the inner wall of the cover 2 detects that the internal pressure of the tank 1 has reached the set standard. Alternatively, the displacement sensor 21 connected to the airbag 10 gradually approaches the cover 2 and reaches the set standard. Or, when the pressure sensor 31 is activated, the timing sensor 24 is activated simultaneously. At this time, the timing sensor 24 times the time. Regardless of which detection method reaches the standard, the exhaust pipe of the tank 1 is opened, and the steam inside the tank 1 will be discharged from the exhaust pipe. Subsequently, the internal pressure of the tank 1 gradually returns to standard atmospheric pressure. During the process of the tank 1 returning to standard atmospheric pressure, the internal pressure of the tank 1 is no longer sufficient to push the piston 34 to the bottom. Therefore, the tension spring 25 located between the piston 34 and the receiving tube 4 will gradually contract, thereby pulling the piston 34 back to its original position. During the upward movement of piston 34, the upper surface of piston 34 will first abut against the bottom surface of T-ring 30 of inner tube 29 (e.g., Figure 11 As shown, the inner diameter of the T-ring 30 is smaller than the inner diameter of the inner tube 29, which can lift the inner tube 29 up until it is completely inside the outer tube 5. At the same time, the tension spring 25 will be stored in the storage cavity 33 of the storage tube 4, and the pressure inside the tank 1 will be restored to the standard atmospheric pressure. Then, according to the sterilization requirements, the above steps can be repeated several times until sterilization is completed. Finally, it is important to note that: 1. After the culture of bacteria in tank 1 is completed, it is necessary to clean and sterilize. After this culture is completed and before the next culture, it is necessary to carry out a unified inspection of tank 1 and other components. If components such as airbag 10, displacement sensor 21, and timing sensor 24 malfunction, the airbag 10 and other components can be separated from the cover 2 by separating the bolts between the mounting block 22 and the cover 2. 2. If the ultraviolet lamp 13 malfunctions during maintenance, it can be repaired or replaced by separating the connecting flange 14 between the connecting rod 12 and the extension rod 32.

[0041] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A strain tank with double sterilization methods, comprising a tank body (1), a cover body (2) installed on the tank body (1), a stirring motor (3) installed on the cover body (2), a stirring rod (8) installed on the output shaft of the stirring motor (3) and located inside the tank body (1), and a discharge pipe (9) installed at the bottom of the tank body (1), characterized in that: The bottom surface of the tank body (1) is fixedly connected with a steam pipe (7), the upper surface of the cover body (2) is provided with a fixed opening (16) penetratingly formed, the inner wall of the fixed opening (16) is connected with an extension assembly, the other end of the extension assembly penetrates into the inside of the tank body (1) and is connected with an ultraviolet lamp (13), the ultraviolet lamp (13) is sleeved outside the stirring rod (8), the top surface of the inner wall of the cover body (2) is connected with an expansion assembly, one end of the expansion assembly is communicated with the end of the extension assembly away from the ultraviolet lamp (13), the steam pipe (7) is communicated with the inside of the tank body (1), one end of the steam pipe (7) located outside the tank body (1) is provided with a valve, and the top surface of the inner wall of the cover body (2) is fixedly connected with a pressure sensor (31).

2. The strain tank with double sterilization methods according to claim 1, characterized in that: The top surface of the inner wall of the cover body (2) is provided with a mounting cavity (15), and the expansion assembly is located in the mounting cavity (15).

3. The strain tank with double sterilization methods according to claim 2, characterized in that: The expansion assembly comprises an air bag (10), a semicircular plate (19) and a connecting pipe (23), the semicircular plate (19) is located in the mounting cavity (15), the bottom surface of the semicircular plate (19) is fixedly connected with the upper surface of the air bag (10), one end of the air bag (10) is fixedly connected with one end of the connecting pipe (23), the other end of the connecting pipe (23) is connected with the extension assembly, and the air bag (10) is communicated with the connecting pipe (23).

4. The culture tank with double sterilization method according to claim 3, characterized in that: A plurality of mounting openings (18) are formed in one side of the mounting cavity (15), a plurality of mounting blocks (22) are fixedly connected with the side wall of the semicircular plate (19), a plurality of mounting blocks (22) are located in a plurality of mounting openings (18) respectively, the mounting blocks (22) are detachably connected with the cover body (2) through bolts, and the end of the air bag (10) away from the semicircular plate (19) is provided with an opening (11) penetratingly formed.

5. The double sterilization method of a culture tank according to claim 4, wherein: The same side of the semicircular plate (19) and the air bag (10) is fixedly connected with a supporting block (20), the upper surface of the supporting block (20) close to the air bag (10) is fixedly connected with a displacement sensor (21), and the bottom surface of the supporting block (20) close to the semicircular plate (19) is fixedly connected with a timing sensor (24).

6. The double sterilization method of a culture tank according to claim 5, wherein: The telescopic assembly comprises an outer tube (5), a connecting rod (12), an L-shaped tube (28), an inner tube (29), a T-shaped ring (30), an extension rod (32) and a piston (34), the outer wall of the outer tube (5) is fixedly connected with the inner wall of a fixed port (16), the inner walls of the outer tube (5) and the inner tube (29) are both provided with air cavities (26), the lower end of the extension rod (32) is fixedly connected with the outer wall of an ultraviolet lamp (13), the upper end of the extension rod (32) is connected with the lower end of the connecting rod (12), the upper end of the connecting rod (12) penetrates into the air cavity (26) of the inner tube (29) and is fixedly connected with the piston (34), the piston (34) is slidably connected with the air cavity (26) of the inner tube (29), the upper end of the inner tube (29) penetrates into the inside of the outer tube (5) and is fixedly connected with the bottom surface of the T-shaped ring (30), the outer wall of the inner tube (29) and the outer wall of the T-shaped ring (30) are both slidably connected with the air cavity (26) of the outer tube (5), one side of the upper end of the outer tube (5) is fixedly connected with one end of the L-shaped tube (28), the other end of the L-shaped tube (28) is fixedly connected with one end of a connecting tube (23) away from an air bag (10), and the connecting tube (23), the L-shaped tube (28) and the air cavity (26) are in communication, and the diameter of the outer wall of the piston (34) is greater than the diameter of the inner wall of the T-shaped ring (30).

7. The double sterilization method of a culture tank according to claim 6, wherein: A connecting flange (14) is arranged between the upper end of the extension rod (32) and the lower end of the connecting rod (12), and the extension rod (32) and the connecting rod (12) are detachably connected through the connecting flange (14).

8. The double sterilization method of a culture tank according to claim 7, wherein: A connecting port (17) is formed in one side of the fixed port (16), the inner wall of the connecting port (17) is fixedly connected with a side tube (6), one side of the side tube (6) is fixedly connected with the side wall of the upper end of the outer tube (5), and the bottom surface of the side tube (6) is provided with a butt joint port (27), and the connecting tube (23) and the L-shaped tube (28) are both located in the butt joint port (27).

9. The double sterilization method of a culture tank according to claim 8, wherein: A receiving tube (4) is fixedly connected to the upper surface of the outer tube (5), a receiving cavity (33) is formed in the inside of the receiving tube (4), a tension spring (25) is fixedly connected to the top surface of the receiving cavity (33), and the other end of the tension spring (25) penetrates through the air cavity (26) and is fixedly connected with the upper surface of the piston (34).

10. The culture tank with double sterilization method according to claim 9, characterized in that: The number of the outer tubes (5) is not limited to one, and the connecting relationship between two adjacent outer tubes (5) is the same as the connecting relationship between the outer tube (5) and the inner tube (29).

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