Sterile inoculation operation device for plant cell group solid culture

Abstract

The invention discloses a sterile inoculation operation device for plant cell group solid culture, and belongs to the technical field of plant culture, the sterile inoculation operation device comprises a shell module and knife shell modules, the shell module is internally provided with a locking cavity and a storage cavity which are opposite to each other, and the two knife shell modules are alternately assembled in the locking cavity; according to the invention, the circulating switching of the two groups of knife shell modules between an operation state and a disinfection state is realized through an alternate working mechanism, one group executes a cutting task, and the other group synchronously carries out disinfection preparation, so that the operation interval time is greatly shortened, the process interruption caused by pollution or replacement of the knife is avoided, and the working efficiency is improved. Through modular design and automatic rotation, the system remarkably improves the operation efficiency, cleanliness reliability and flow continuity of a sterile inoculation link in plant cell group solid culture, and is suitable for plant biotechnology experiments and production environments with high repeatability and high cleanliness requirements.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of plant cultivation, in particular to a sterile inoculation operation device for plant cell group solid culture. BACKGROUND

[0002] The plant cell group solid culture inoculation device is a core equipment for transferring explants such as shoot tips, leaves and seeds to a solid culture medium under sterile conditions in plant tissue culture.

[0003] However, the existing inoculation equipment has poor operation continuity in actual operation process, and needs to be interrupted after each use to sterilize or replace the knife, resulting in long operation interval time, which is difficult to meet the needs of high repeatability experiments or production. When the knife is replaced or sterilized, external pollution is easily introduced, the stability of the sterile environment is affected, the inoculation success rate is reduced, and manual intervention is relied on for knife replacement and positioning, which not only reduces the efficiency, but also increases the operation complexity and human error. SUMMARY

[0004] In view of the deficiencies in the prior art, the purpose of the embodiments of the present application is to provide a sterile inoculation operation device for plant cell group solid culture to solve the problems in the background art.

[0005] To achieve the above-mentioned purpose, the present application provides the following technical scheme:

[0006] A sterile inoculation operation device for plant cell group solid culture comprises a shell module and a knife shell module;

[0007] The shell module is internally provided with opposite locking cavities, a first storage cavity and a second storage cavity, and two groups of knife shell modules are movably assembled in the shell module;

[0008] The two groups of knife shell modules are alternately assembled in the locking cavities, and the remaining one group of knife shell modules is stored in any one of the first storage cavity and the second storage cavity.

[0009] As a further scheme of the present application, the shell module comprises a shell assembly, the shell assembly comprises a front section bin, a tail section bin, a front section chute and a tail baffle, the tail section bin is communicated with one side of the front section bin, the tail section bin is provided with opposite first and second storage cavities, a plurality of front section chutes are arranged on the inner cavity wall surface of the front section bin, and a tail baffle is further fixedly arranged at one end of the tail section bin.

[0010] As a further scheme of the present application, the shell assembly further comprises a tail section chute and a longitudinal chute, the tail section chute is arranged on one side of the inner cavity wall of the tail section bin, and the tail section chute is communicated with the front section chute, and two longitudinal chutes are vertically arranged on the tail section chute.

[0011] As a further scheme of the present application, the shell module further comprises a transmission assembly, the transmission assembly comprises a transmission shaft, a transmission gland, a longitudinally arranged limiting groove, a laterally arranged limiting groove and an avoiding groove, the transmission shaft is slidingly inserted into the tail section bin, the transmission gland is slidingly arranged in the tail section bin and fixedly connected with the transmission shaft, the inner cavity top surface of the transmission gland is provided with the longitudinally arranged limiting groove, the inner cavity side surface of the transmission gland is provided with the laterally arranged limiting groove, and two groups of avoiding grooves are arranged on the transmission gland and aligned with the longitudinal sliding groove.

[0012] As a further scheme of the present application, the knife shell module comprises a knife shell, a longitudinally arranged protruding groove, a laterally arranged protruding groove, a lateral protruding key, an abutment plate and a hollow cavity, one end of the knife shell is fixedly assembled with a sterilization module, the outer diameter end of the knife shell is provided with the longitudinally arranged protruding groove and the laterally arranged protruding groove, the longitudinally arranged protruding groove is arranged at the top and bottom of the knife shell, the laterally arranged protruding groove is arranged at the side of the knife shell, two groups of outward protruding lateral protruding keys are arranged on one side of the laterally arranged protruding groove, the longitudinally arranged protruding groove and the laterally arranged protruding groove are slidingly arranged in the front section sliding groove, the lateral protruding key is slidingly arranged in the tail section sliding groove and the longitudinal sliding groove, and the abutment plate is arranged at the end of the knife shell, and the middle part of the abutment plate is provided with the hollow cavity.

[0013] As a further scheme of the present application, the knife head assembly further comprises an unlocking column, a locking column, an inclined sliding groove and an unlocking sliding pin, the unlocking column is slidingly inserted into the abutment plate, the locking column is slidingly arranged on the inner side of the abutment plate, the middle part of the locking column is provided with the inclined sliding groove, one end of the unlocking sliding pin is fixedly connected with the unlocking column, and the other end of the unlocking sliding pin abuts against the inclined sliding groove.

[0014] As a further scheme of the present application, the sterile inoculation operation device for solid culture of plant cells further comprises a driving assembly, the driving assembly comprises a main traction rod, a taper head and a ring-shaped groove, the main traction rod is fixedly arranged in the front section bin and the tail section bin, one end of the main traction rod is slidingly inserted into the tail baffle, the other end of the main traction rod is provided with the taper head, and one side of the taper head is further provided with the ring-shaped groove.

[0015] As a further scheme of the present application, the driving assembly further comprises a main driving rod, a one-way tooth groove, a ratchet wheel, a first transmission wheel, a second transmission wheel, a first transmission bevel gear and a second transmission bevel gear, the main driving rod is fixedly assembled at the end of the main traction rod, one side of the main driving rod is provided with the one-way tooth groove, the ratchet wheel is fixedly arranged on one side of the tail section bin and is in meshing connection with the one-way tooth groove, one end of the first transmission wheel is coaxially connected with the ratchet wheel, the other end of the first transmission wheel is in transmission connection with the second transmission wheel, the first transmission bevel gear is coaxially connected with the second transmission wheel, and the second transmission bevel gear is in meshing connection with the first transmission bevel gear.

[0016] As a further scheme of the present application, the driving assembly further comprises a residual gear, a transmission frame and a driven tooth surface, the residual gear is arranged on one side of the tail section bin, and one end of the residual gear is coaxially fixedly connected with the second transmission bevel gear, the transmission frame is slidably arranged on one side of the tail section bin and fixedly connected with the two groups of transmission shafts, and the inner frame side of the transmission frame is provided with two groups of driven tooth surfaces, and the driven tooth surfaces are in engagement with the transmission frame.

[0017] In summary, the embodiment of the present application has the following beneficial effects compared with the prior art:

[0018] The present application realizes the cyclic switching between the working and disinfection states of the two groups of cutter shell modules through the alternate working mechanism, one group performs the cutting task, and the other group synchronously performs the disinfection preparation, so that the operation interval time is greatly shortened, the process interruption caused by cutter pollution or replacement is avoided, through the modular design and automatic rotation, the system significantly improves the operation efficiency, cleanliness reliability and process continuity of the aseptic inoculation link in the plant cell group solid culture, and is suitable for the plant biotechnology experiment and production environment with high repeatability and high cleanliness requirements.

[0019] In order to more clearly set forth the structural features and effects of the present application, the present application will be described in detail below in combination with the drawings and specific embodiments. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 It is a partial sectional view of the aseptic inoculation operation device for plant cell group solid culture in the embodiment of the present application.

[0021] Figure 2 It is a side structure schematic view of the aseptic inoculation operation device for plant cell group solid culture in the embodiment of the present application.

[0022] Figure 3 It is Figure 2 It is an enlarged schematic view of reference sign A.

[0023] Figure 4 It is Figure 2 It is an enlarged schematic view of reference sign B.

[0024] Figure 5 It is a side structure schematic view of the aseptic inoculation operation device for plant cell group solid culture in the embodiment of the present application.

[0025] Figure 6 It is Figure 5 It is an enlarged schematic view of reference sign C.

[0026] Figure 7 It is a partial sectional view of the cutter shell body in the aseptic inoculation operation device for plant cell group solid culture in the embodiment of the present application.

[0027] Figure 8 For Figure 7 An enlarged schematic view of the reference D.

[0028] Reference: 1 - mold shell assembly, 101 - front section bin, 102 - tail section bin, 103 - front section chute, 104 - tail baffle, 105 - tail section chute, 106 - longitudinal chute, 2 - transmission assembly, 201 - transmission shaft, 202 - transmission gland, 203 - longitudinally arranged limiting groove, 204 - laterally arranged limiting groove, 205 - avoidance groove, 3 - tool bit assembly, 301 - tool shell, 302 - longitudinally arranged convex groove, 303 - laterally arranged convex groove, 304 - lateral key, 305 - abutment plate, 306 - hollow cavity, 307 - unlocking column, 308 - locking column, 309 - inclined chute, 310 - unlocking slide pin, 4 - drive assembly, 401 - main traction rod, 402 - taper head, 403 - annular groove, 404 - main drive rod, 405 - one-way tooth groove, 406 - ratchet wheel, 407 - first transmission wheel, 408 - second transmission wheel, 5 - inoculation tool bit, 6 - disinfection module. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical scheme and advantages of the present application more clear, the present application is further described in detail below in combination with the drawings and examples. It should be understood that the specific examples described herein are only used to explain the present application and do not limit the present application.

[0030] The specific implementation of the present application is described in detail below in combination with specific examples.

[0031] Referring to Figures 1-8 A sterile inoculation operation device for plant cell group solid culture, the sterile inoculation operation device for plant cell group solid culture has opposite first direction x, second direction y and third direction z, the sterile inoculation operation device for plant cell group solid culture includes shell module and tool shell module; the shell module is internally provided with opposite locking cavity, first storage cavity a1 and second storage cavity a2, and two groups of tool shell modules are movably assembled in the shell module; the two groups of tool shell modules are alternately assembled in the locking cavity, and the remaining group of tool shell modules is stored in any one of the first storage cavity a1 and the second storage cavity a2.

[0032] In actual use, the sterile inoculation device is used for plant cell group solid culture process, which is composed of a shell module and a knife shell module. The shell module is precisely designed and integrated with a set of locking cavities and two sets of independent storage cavities. The locking cavity, as the core working positioning area of the knife shell module, has a mechanical locking function, which can ensure the position stability and operation precision of the knife shell module during sterile cutting. The two sets of storage cavities are used to store the standby knife shell modules in standby or temporary storage state respectively, realizing the rotation management and operation continuity of the modules. On the side of each storage cavity, an independent sterilization module is configured. The module performs real-time or periodic sterilization treatment on the execution unit of the knife shell module in the cavity through ultraviolet or spray sterilization, to ensure that it meets the sterile standard before entering the working state. Taking the first storage cavity as an example, when a set of knife shell modules is placed in the locking cavity to perform sterile cutting and separation of plant cell tissues, another set of knife shell modules is placed in the first storage cavity a1 and simultaneously subjected to whole-process sterilization treatment by the sterilization module. After the end of the previous operation period and the completion of the cutting task by the knife shell module, the system precisely presses and transfers the used knife shell module to the second storage cavity a2 for temporary storage through an external driving source such as a pneumatic push rod or an electric linear actuator. At the same time, the other set of knife shell modules that have completed sterilization in the first storage cavity are automatically delivered to the locking cavity along the preset guide rail under the action of the same driving mechanism, to continue the next round of sterile inoculation operation. This alternating working mechanism realizes the cyclic switching of the two sets of knife shell modules between the working and sterilization states. One set performs cutting task while the other set is simultaneously prepared for sterilization, thereby greatly shortening the operation interval time and avoiding process interruption caused by tool contamination or replacement. Through modular design and automatic rotation, the system significantly improves the operation efficiency, cleanliness reliability and process continuity of the sterile inoculation link in plant cell group solid culture, and is suitable for plant biotechnology experiments and production environments with high repeatability and high cleanliness requirements.

[0033] Please refer to Figure 2 and Figure 4 A sterile inoculation device for plant cell group solid culture, the shell module comprises a mold shell assembly 1, the mold shell assembly 1 comprises a front section bin 101, a tail section bin 102, a front section chute 103 and a tail baffle 104, one side of the front section bin 101 is communicated with the tail section bin 102, the tail section bin 102 is provided with opposite first storage cavity a1 and second storage cavity a2, and the inner cavity wall surface of the front section bin 101 is provided with a plurality of front section chutes 103. One end of the tail section bin 102 is also fixedly provided with a tail baffle 104.

[0034] In actual use, the tail section warehouse 102 is internally provided with opposite first storage cavities a1 and second storage cavities a2, the two groups of storage cavities are arrayed in the third direction z, and one side of the two groups of storage cavities is provided with a disinfection module 6 for disinfecting the knife group module. The front section warehouse 101 is provided as a locking cavity for positioning the knife group module, so that after any one group of knife group modules enters the locking cavity, the other group of knife group modules is located in the first storage cavity a1 or the second storage cavity a2, and the inoculation knife head 5 on one side faces the disinfection module 6. At this time, by continuously applying ultraviolet light sterilization, the bacteria remaining on the inoculation knife head 5 can be effectively removed. The inner wall side of the front section warehouse 101 is further provided with a plurality of front section sliding grooves 103. The front section sliding grooves 103 are connected with the knife group module limiting sliding to limit the installation position of the knife group module and prevent the knife group module from rotating in the front section warehouse 101. The tail baffle 104 is used to limit the position of the knife group module during the retreat process.

[0035] Further, the shell assembly 1 further comprises a tail section sliding groove 105 and a longitudinal sliding groove 106. The tail section sliding groove 105 is arranged on one side of the inner cavity wall of the tail section warehouse 102, and the tail section sliding groove 105 is connected with the front section sliding groove 103. Two groups of longitudinal sliding grooves 106 are vertically arranged on the tail section sliding groove 105. The tail section sliding groove 105 is matched with the limiting groove on one side of the knife group module to limit the sliding track of the knife group module in the tail section warehouse 102. The longitudinal sliding groove 106 on one side of the tail section sliding groove 105 is used to limit the sliding track of the knife group module in the third direction z.

[0036] Please refer to Figure 4 A sterile inoculation operation device for plant cell group solid culture, the shell module further comprises a transmission assembly 2, the transmission assembly 2 comprises a transmission shaft 201, a transmission gland 202, a longitudinally arranged limiting groove 203, a laterally arranged limiting groove 204 and an avoidance groove 205, the transmission shaft 201 is slidingly inserted into the tail section warehouse 102, the transmission gland 202 is slidingly arranged in the tail section warehouse 102 and is fixedly connected with the transmission shaft 201, the inner cavity top surface of the transmission gland 202 is provided with the longitudinally arranged limiting groove 203, the inner cavity side surface of the transmission gland 202 is provided with the laterally arranged limiting groove 204, and the transmission gland 202 is further provided with two groups of avoidance grooves 205 which are arranged in alignment with the longitudinal sliding grooves 106.

[0037] In the embodiment, the transmission shaft 201 is inserted into the guide hole of the tail section chamber 102 in a sliding fit along the third direction Z axis and can be axially displaced along the third direction Z. The end of the transmission shaft 201 close to the inner cavity of the tail section chamber 102 is provided with a transmission gland 202, and the two are fixedly connected by fasteners or integrated molding. The top of the transmission gland 202 is provided with a longitudinally extending longitudinal limiting groove 203, and a permanent magnet or an electromagnetic assembly is embedded and fixed in the groove to form a magnetic adsorption structure for adsorbing the knife group module, so that the knife group is stably maintained in the assembled or working state. In addition, the side of the transmission gland 202 is also processed with a side limiting groove 204, and the geometric profile of the groove is matched with the guide flange on the side of the knife group module to form a lateral sliding pair for limiting the freedom degree of the knife group module in the horizontal plane, ensuring its smooth movement along the predetermined track. In addition, the transmission gland 202 is specially designed with a relief groove 205, and the projection position of the groove along the first direction X axis is completely aligned with the longitudinal sliding groove 106 on the tail section chamber 102. Through this structural design, when the transmission gland 202 reciprocally slides along the third direction Z axis, the relief groove 205 can effectively avoid the solid structure of the longitudinal sliding groove 106, avoiding mechanical interference with the sliding groove edge during movement, so as to ensure smooth operation of the transmission assembly and maintain the accuracy and reliability of the overall movement of the system.

[0038] Please refer to Figure 3 and Figure 8 A sterile inoculation operation device for solid culture of plant cells, the knife shell module comprises a knife shell 301, a longitudinal groove 302, a side groove 303, a side key 304, an abutment plate 305 and a hollow cavity 306, one end of the knife shell 301 is fixedly provided with an inoculation knife head 5, the outer diameter end of the knife shell 301 is provided with a longitudinal groove 302 and a side groove 303, the longitudinal groove 302 is arranged at the top and bottom of the knife shell 301, the side groove 303 is arranged at the side of the knife shell 301, and two groups of outward protruding side keys 304 are arranged on one side of the side groove 303, the longitudinal groove 302 and the side groove 303 are limitingly and slidably arranged in the front section sliding groove 103, the side keys 304 are limitingly and slidably arranged in the tail section sliding groove 105 and the longitudinal sliding groove 106, and the tail end of the knife shell 301 is further provided with an abutment plate 305, and the middle of the abutment plate 305 is provided with a hollow cavity 306.

[0039] In the present embodiment, the front end of the cutter shell 301 is fixedly installed with the inoculation cutter head 5 by interference fit or fasteners. On the outer wall side of the cutter shell 301, a longitudinal groove 302 is arranged in the axial direction thereof, which is distributed on the top and bottom surfaces of the cutter shell 301, and a lateral groove 303 is arranged in the lateral wall direction thereof. The longitudinal groove 302 is located at one end close to the transmission gland 202, and a permanent magnet or soft magnetic material corresponding to the polarity of the magnetic body is embedded in the groove, so that when the cutter shell 301 slides close to the transmission gland 202 in the first direction x, the longitudinal groove 302 at the end can be firmly attracted to the corresponding longitudinal limiting groove 203 on the transmission gland 202 by magnetic attraction, achieving axial positioning and temporary fixation. The lateral groove 303 is arranged in the radial direction of the cutter shell 301 and is slidingly fitted in the front segment sliding groove 103 of the device. Further, a radially protruding lateral key 304 is arranged outside the lateral groove 303, which simultaneously forms a sliding fit with the front segment sliding groove 103 and the tail segment sliding groove 105. When the cutter group module in the front segment magazine 101 is retracted to the tail segment magazine 102 area in the negative direction of the x axis, the cutter shell 301 is constrained to slide in the tail segment sliding groove 105 by the lateral key 304, ensuring the stability of its movement in the horizontal direction. When the cutter shell 301 continues to retract until its end face contacts the tail baffle 104, the two groups of lateral keys 304 are respectively aligned with the two groups of longitudinal sliding grooves 106 correspondingly arranged in the tail segment magazine 102. At this time, the cutter shell 301 is released from the horizontal direction constraint and can be accurately moved in the vertical direction, i.e., the third direction z, along the longitudinal sliding groove 106, realizing the space conversion of the cutter head between the working position and the recovery position, and completing the multi-degree-of-freedom positioning operation.

[0040] Further, the tool head assembly 3 further comprises an unlocking column 307, a locking column 308, an inclined sliding groove 309 and an unlocking sliding pin 310. The unlocking column 307 is slidingly arranged on the abutment plate 305. The locking column 308 is elastically slidingly arranged on the inner side of the abutment plate 305. The middle part of the locking column 308 is provided with the inclined sliding groove 309. One end of the unlocking sliding pin 310 is fixedly connected with the unlocking column 307, and the other end is in dynamic abutment with the inclined sliding groove 309. The unlocking column 307 is axially installed at the end of the abutment plate 305, and one radial side thereof is fixedly connected with the unlocking sliding pin 310. The locking column 308 is axially slidingly inserted into the abutment plate 305 under the support of the elastic element, and one end thereof penetrates through the abutment plate 305 and extends into the hollow cavity 306. The middle section of the locking column 308 is processed with the inclined sliding groove 309, and the unlocking sliding pin 310 is always in sliding contact with the surface of the inclined sliding groove 309. When an external force is applied to the unlocking column 307 along the X-axis direction, the unlocking column 307 translates towards the inside of the abutment plate 305, driving the unlocking sliding pin 310 to move synchronously. The unlocking sliding pin 310 slides along the inclined guide surface of the inclined sliding groove 309, decomposes the axial thrust into a radial component perpendicular to the axis of the locking column 308, thereby driving the locking column 308 to retract along the Y-axis direction away from the hollow cavity 306. With the end of the locking column 308 completely out of the internal space of the hollow cavity 306, the main traction rod 401 originally restricted by the locking column 308 is mechanically released and can freely move along the Z-axis direction. The mechanism converts the axial input displacement into a radial locking release motion through the inclined surface transmission mechanism formed by the inclined sliding groove 309 and the unlocking sliding pin 310, and finally realizes the axial free release of the main traction rod 401, completing the unlocking process.

[0041] Please refer to Figure 8 The sterile inoculation operation device for plant cell group solid culture further comprises a driving assembly 4. The driving assembly 4 comprises a main traction rod 401, a tapered head 402 and a ring-shaped groove 403. The main traction rod 401 is arranged in the front section bin 101 and the tail section bin 102, and one end of the main traction rod 401 is slidingly arranged on the tail baffle 104. The other end of the main traction rod 401 is provided with the tapered head 402. One side of the tapered head 402 is further provided with the ring-shaped groove 403.

[0042] In this embodiment, the front end of the main traction rod 401 is machined with a tapered structure 402, and the rod body behind the tapered head is provided with a ring groove 403. When the main traction rod 401 moves along the positive direction of the X axis and abuts to the abutting plate 305, the tapered head 402 is inserted into the hollow cavity 306, the tapered surface cooperates with the cavity structure, and the knife shell 301 is pushed to slide along the guide rail composed of the pre-set tail section sliding groove 105 and the front section sliding groove 103, when the knife shell 301 moves along the rail to the pre-set locking cavity position, the end of the tapered head 402 contacts with the locking column 308, and continuously applies pressure to push the locking column 308 to overcome the pre-tightening force of the internal elastic element, and slide back along the Y axis away from the center of the hollow cavity 306. As the main traction rod 401 continues to advance, until the tapered head 402 is completely seated in the specified position inside the hollow cavity 306, the locking column 308 moves along the Y axis to the center under the action of elastic restoring force, and the front end is accurately clamped into the ring groove 403 on the rod body of the main traction rod 401, forming mechanical interlocking. This state effectively limits the accidental withdrawal of the main traction rod 401 along the negative direction of the X axis from the hollow cavity 306, when the main traction rod 401 needs to be withdrawn, it moves along the negative direction of the X axis. At this time, the side wall of the ring groove 403 is clamped with the locking column 308, and the friction force generated and the geometric constraint will drive the locking column 308 and the knife shell 301 fixed thereto to move synchronously along the negative direction of the X axis, realizing the recovery of the knife shell 301 on the rail, and the knife shell 301 slides to the end of the rail, and the unlocking column 307 on the abutting plate 305 is in contact and pressure connection with the tail baffle 104 fixed at the end of the structure. As the main traction rod 401 continues to withdraw, the unlocking column 307 bears the thrust along the X axis direction under the block of the tail baffle 104. The thrust is transmitted to the unlocking slide pin 310 fixed thereto through the unlocking column 307, and the unlocking slide pin 310 slides along the inclined sliding groove 309. The axial thrust is converted into radial force by the inclined surface, forcing the locking column 308 to retract again along the Y axis away from the main traction rod 401, so that the front end is completely separated from the ring groove 403. After the locking is released, the mechanical connection between the main traction rod 401 and the knife shell 301 is released. The main traction rod 401 can continue to move freely along the negative direction of the X axis, and the withdrawal is completed. While the released knife shell 301 is physically blocked by the tail baffle 104 and stays at the specified position of the tail section magazine 102. The knife shell 301 will remain stationary until the subsequent another group of knife module moves along the X axis direction, and pushes it out of the tail section magazine 102 position and to the storage cavity on the other side of the system, completing the transfer and storage process of the knife shell. The whole mechanism realizes the continuous automatic operation of traction, locking, synchronous movement, end triggering unlocking and modular transfer.

[0043] Please refer to Figure 6The sterile inoculation operation device for plant cell group solid culture comprises a driving assembly 4, and the driving assembly 4 further comprises a main driving rod 404, a one-way tooth groove 405, a ratchet wheel 406, a first transmission wheel 407, a second transmission wheel 408, a first transmission bevel gear 409 and a second transmission bevel gear 410, the main driving rod 404 is fixedly arranged at the end of the main traction rod 401, one side of the main driving rod 404 is provided with the one-way tooth groove 405, the ratchet wheel 406 is arranged on one side of the tail section bin 102 in a shaft manner, and the ratchet wheel 406 is in meshing connection with the one-way tooth groove 405, one end of the first transmission wheel 407 is coaxially connected with the ratchet wheel 406, the other end of the first transmission wheel 407 is in transmission connection with the second transmission wheel 408, the first transmission bevel gear 409 is coaxially connected with the second transmission wheel 408, and the second transmission bevel gear 410 is in meshing connection with the first transmission bevel gear 409.

[0044] In actual use, the main driving rod 404 is driven by an external driving source, and the specific driving form and structure are not limited in the description. The main driving rod 404 performs reciprocating linear motion in the first direction x; when it moves in the negative x direction and drags the main traction rod 401 to move synchronously, the process can be divided into two stages. In the first stage, the displacement of the main traction rod 401 in the negative x direction first drags the cutter shell 301 to closely abut against the tail baffle 104. The abutting action eliminates the axial gap between the cutter shell 301 and the main traction rod 401, and at the same time triggers the built-in locking mechanism between the two to be released, so that the main traction rod 401 and the cutter shell 301 are decoupled in power at this position, and then enters the second stage. The main traction rod 401 continues to move in the negative x direction under the continuous traction of the main driving rod 404, and at this time, the one-way tooth groove 405 arranged on the main traction rod 401 and the ratchet wheel 406 enter the meshing state and produce abutment. Under the action of the meshing force, the ratchet wheel 406 rotates in one direction around its axis, and the rotational motion of the ratchet wheel 406 is directly transmitted to the first transmission wheel 407 through coaxial connection to drive it to rotate synchronously. The first transmission wheel 407 transmits the rotational motion to the second transmission wheel 408 fixed coaxially with the locking column 308 through the synchronous belt transmission mechanism, thereby driving the locking column 308 to rotate around its axis. The end of the locking column 308 is fixedly connected with the first transmission bevel gear 409; when the locking column 308 rotates, the first transmission bevel gear 409 remains in meshing with the second transmission bevel gear 410 and converts the rotational motion by 90 degrees in direction to drive the second transmission bevel gear 410 to rotate in a direction perpendicular to the axis of the locking column 308. The transmission chain completely realizes the process of converting the linear reciprocating input of the main driving rod 404 into one-way rotation through the ratchet wheel 406, and then driving the end execution mechanism through two-stage transmission of the synchronous belt and bevel gear.

[0045] Further, the driving assembly 4 further comprises a residual gear 411, a transmission frame 412 and a driven tooth surface 413, the residual gear 411 is arranged on one side of the tail section bin 102, and one end of the residual gear 411 is coaxially fixedly connected with the second transmission bevel gear 410, the transmission frame 412 is slidably arranged on one side of the tail section bin 102 and is fixedly connected with the two groups of transmission shafts 201, the inner frame side of the transmission frame 412 is provided with two groups of driven tooth surfaces 413, the driven tooth surfaces 413 are in meshing connection with the transmission frame 412, the residual gear 411 is fixedly installed on the side wall of the tail section bin 102 through a bearing, the rotation axis thereof coincides with the axis of the second transmission bevel gear 410, and the two are coaxially and rigidly connected. The transmission frame 412 is slidably installed on the same side of the tail section bin 102 through a linear guide rail and is rigidly connected and fixed with the two groups of parallel transmission shafts 201. On both sides of the inner frame of the transmission frame 412, the continuous or segmented driven tooth surfaces 413 are respectively machined, and the tooth surfaces and the gear teeth of the residual gear 411 form a meshing pair. Specifically, when the second transmission bevel gear 410 rotates under the driving of the input power, the residual gear 411 fixedly connected coaxially with the second transmission bevel gear 410 rotates synchronously. Since the gear teeth of the residual gear 411 are not distributed around the whole circumference, the working cycle is as follows:

[0046] S1: When the residual gear segment of the residual gear 411 rotates to enter the meshing state with the driven tooth surface 413 on the first side of the transmission frame 412, the rotational movement of the gear will be converted into a linear driving force for the transmission frame 412 along the positive direction of the third direction z axis through the meshing of the tooth surface, so as to push the transmission frame 412 and the fixed components thereof to slide in this direction.

[0047] S2: As the residual gear 411 continues to rotate, the residual gear segment thereof is separated from the first side tooth surface, passes through the gearless idling interval, and then enters the meshing with the driven tooth surface 413 on the second side of the transmission frame 412. At this time, the meshing drives the transmission frame 412 to slide in the negative direction of the third direction z, realizing reverse movement.

[0048] Through the above mechanism, the continuous rotation of the residual gear 411 is converted into the periodic reciprocating linear motion of the transmission frame 412 along the z axis. Since the transmission frame 412 is rigidly connected with the two groups of transmission shafts 201, the transmission shafts 201 and the transmission gland 202 installed at the end thereof will synchronously follow the transmission frame 412 to accurately reciprocate in the z axis direction. In addition, when the main driving rod 404 moves in the positive direction of the first direction x, the one-way tooth groove 405 on the side wall thereof is in a non-meshing or overrunning state with the ratchet wheel 406 mechanism in the system, and no effective power transmission or mechanical interference occurs between the two, so as to ensure that the reciprocating transmission mechanism of the driving assembly 4 independently operates and does not interfere with each other in this movement mode.

[0049] The above merely describes preferred embodiments of the present application, and is not used to limit the present application, any modification, equivalent replacement and improvement within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. A sterile inoculation device for solid culture of plant cell groups, characterized in that, The aseptic inoculation device for solid culture of plant cell groups includes: Housing module and blade housing module; The housing module has a locking cavity, a first storage cavity and a second storage cavity inside, and two sets of blade housing modules are movably assembled inside the housing module. Two sets of blade housing modules are alternately assembled in the locking cavity, and the remaining set of blade housing modules is stored in any one of the first and second storage cavities.

2. The aseptic inoculation device for solid culture of plant cell groups according to claim 1, characterized in that, The housing module includes a housing assembly, which includes a front section compartment, a rear section compartment, a front section chute, and a rear baffle. The front section compartment is connected to the rear section compartment on one side. The rear section compartment has a first storage cavity and a second storage cavity with opposite orientations. The inner wall of the front section compartment has several front section chutes. The rear section compartment also has a fixed rear baffle at one end.

3. The aseptic inoculation device for solid culture of plant cell groups according to claim 2, characterized in that, The mold shell assembly also includes a tail section slide groove and a longitudinal slide groove. The tail section slide groove is arranged on one side of the inner wall of the tail section compartment and is connected to the front section slide groove. Two sets of longitudinal slide grooves are also vertically arranged on the tail section slide groove.

4. The aseptic inoculation device for solid culture of plant cell groups according to claim 3, characterized in that, The housing module also includes a transmission assembly, which includes a transmission shaft, a transmission cover, a longitudinal limiting groove, a lateral limiting groove, and a clearance groove. The transmission shaft is slidably inserted into the tail section compartment, and the transmission cover is slidably disposed in the tail section compartment and fixedly connected to the transmission shaft. The top surface of the inner cavity of the transmission cover is provided with a longitudinal limiting groove, and the side surface of the inner cavity of the transmission cover is provided with a lateral limiting groove. The transmission cover is also provided with two sets of clearance grooves aligned with the longitudinal sliding groove.

5. The aseptic inoculation device for solid culture of plant cell groups according to claim 1, characterized in that, The blade housing module includes a blade housing, a longitudinal protrusion, a lateral protrusion, a lateral key, a stop plate, and a hollow cavity. A disinfection module is fixedly mounted at one end of the blade housing. The outer diameter end of the blade housing is provided with a longitudinal protrusion and a lateral protrusion. The longitudinal protrusion is located at the top and bottom of the blade housing, and the lateral protrusion is located on the side of the blade housing. Two sets of outwardly protruding lateral keys are also provided on one side of the lateral protrusion. The longitudinal protrusion and the lateral protrusion are slidably arranged in the front section slide groove, and the lateral key is slidably arranged in the tail section slide groove and the longitudinal slide groove. The end of the blade housing is also provided with a stop plate, and a hollow cavity is provided in the middle of the stop plate.

6. The aseptic inoculation device for solid culture of plant cell groups according to claim 1, characterized in that, The cutter head assembly also includes an unlocking post, a locking post, an oblique groove, and an unlocking pin. The unlocking post is slidably inserted into the abutment plate, the locking post is elastically slidably arranged inside the abutment plate, the locking post has an oblique groove in the middle, one end of the unlocking pin is fixedly connected to the unlocking post, and the other end of the unlocking pin abuts against the oblique groove.

7. The aseptic inoculation device for solid culture of plant cell groups according to claim 1, characterized in that, The aseptic inoculation device for solid culture of plant cell groups also includes a drive assembly, which includes a main traction rod, a cone head, and an circumferential groove. The main traction rod is fixedly arranged in the front section and the tail section, and one end of the main traction rod is slidably inserted into the tail baffle. The other end of the main traction rod is provided with a cone head, and an circumferential groove is also provided on one side of the cone head.

8. The aseptic inoculation device for solid culture of plant cell groups according to claim 1, characterized in that, The drive assembly further includes a main drive rod, a one-way toothed groove, a ratchet, a first transmission wheel, a second transmission wheel, a first transmission bevel gear, and a second transmission bevel gear. The main drive rod is fixedly mounted to the end of the main traction rod. A one-way toothed groove is provided on one side of the main drive rod. The ratchet is fixedly arranged on one side of the tail section compartment, and the ratchet meshes with the one-way toothed groove. One end of the first transmission wheel is coaxially connected to the ratchet, and the other end of the first transmission wheel is connected to the second transmission wheel. The first transmission bevel gear and the second transmission wheel are coaxially connected, and the second transmission bevel gear meshes with the first transmission bevel gear.

9. The aseptic inoculation device for solid culture of plant cell groups according to claim 8, characterized in that, The drive assembly also includes a residual gear, a transmission frame, and driven tooth surfaces. The residual gear is fixedly arranged on one side of the tail section compartment, and one end of the residual gear is coaxially and fixedly connected to the second transmission bevel gear. The transmission frame is slidably arranged on one side of the tail section compartment and fixedly connected to two sets of transmission shafts. Two sets of driven tooth surfaces are provided on the inner frame side of the transmission frame, and the driven tooth surfaces mesh with the transmission frame.

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