Infectly infected toxoplasma gondii repellent and preparation method thereof
The expellant prepared by combining artemisinin extract and other components solves the problems of single efficacy and unstable composition in existing technologies, and achieves effective expulsion of Toxoplasma gondii and immune enhancement.
Patent Information
- Application Number
- CN202511504719.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-21
- Publication Date
- 2026-01-02
AI Technical Summary
Current drug treatments for latent toxoplasmosis infection suffer from problems such as limited efficacy, unstable composition, and poor patient compliance, making it difficult to effectively eliminate toxoplasmosis in the long term.
An exfoliant was prepared by combining artemisinin extract, garlic extract, turmeric extract, vitamin C, zinc gluconate, sodium selenite, probiotic powder, and flavoring agents, and through mixing, drying, and granulation processes, forming a synergistic anti-Toxoplasma gondii complex system.
It effectively inhibits Toxoplasma gondii reproduction, reduces the risk of latent infection, improves the host's immune status, enhances the ability to resist reinfection, and has better component stability and uniformity than existing technologies.
Smart Images
Figure CN121243320A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of biopharmaceutical formulation technology, and in particular to a latent Toxoplasma gondii remedy and its preparation method. Background Technology
[0002] Toxoplasmosis is a widespread zoonotic protozoan, and its latent infection has a very high prevalence in the population. Although most infected people have no obvious symptoms, Toxoplasma gondii can remain dormant in tissue cells for a long time, causing the immune system to be continuously and lowly activated, which in turn leads to chronic inflammation, neurological disorders and the risk of infection during pregnancy.
[0003] Current drug treatments mainly rely on combination regimens of sulfonamides or pyrimethamine, but these drugs are mostly used in the acute infection stage, and long-term use can easily lead to liver and kidney burden, increased drug resistance, and intestinal flora imbalance. Furthermore, although some plant extracts have been shown to inhibit Toxoplasma gondii proliferation, the efficacy of single components is limited, and the active substances are easily oxidized or inactivated during formulation processing, making it difficult to achieve long-term stable eradication. Therefore, there is an urgent need to develop an antidote for latent Toxoplasma gondii infections to address the problems of single efficacy, unstable components, and poor patient compliance in existing technologies. Summary of the Invention
[0004] To achieve the above objectives, the present invention provides a latent Toxoplasma gondii remedy and its preparation method.
[0005] A latent Toxoplasma gondii infection remedy comprises artemisinin extract, garlic extract, turmeric extract, vitamin C, zinc gluconate, sodium selenite, probiotic powder, and flavoring agent; wherein the components are expressed in the following percentages by mass:
[0006] Artemisinin extract accounts for 5-10%;
[0007] Garlic extract accounts for 10-15%;
[0008] Turmeric extract accounts for 5-10%;
[0009] Vitamin C accounts for 15-20%;
[0010] Zinc gluconate accounts for 5-10%;
[0011] Sodium selenite accounts for 1-3%;
[0012] Probiotic powder accounts for 30-58.5%;
[0013] Flavoring agent content: 0.5-2%.
[0014] Optionally, the percentages of each component are as follows: artemisinin extract 7%, garlic extract 13%, turmeric extract 7%, vitamin C 17%, zinc gluconate 7%, sodium selenite 2%, probiotic powder 40%, and flavoring agent 1%.
[0015] A method for preparing a repellent for latent Toxoplasma gondii infection includes the following steps:
[0016] S1: Artemisinin extract, garlic extract, turmeric extract, vitamin C, zinc gluconate, sodium selenite, and probiotic powder were sieved separately.
[0017] S2: Artemisinin extract is mixed with the prepared carrier to form premix A;
[0018] S3: Mix garlic extract and turmeric extract to prepare premix B;
[0019] S4: Dissolve vitamin C, zinc gluconate, and sodium selenite in purified water to prepare solution C;
[0020] S5: Mix premix A, premix B and solution C to form a homogeneous main mixture D;
[0021] S6: Add the probiotic powder and flavoring agent to the main mixture D, mix well, and obtain the final mixture E;
[0022] S7: The final mixture E is dried, granulated and shaped to obtain the repellent product.
[0023] Optionally, S1 specifically includes:
[0024] S11: Artemisinin extract is passed through an 80-100 mesh sieve under ambient humidity ≤45%;
[0025] S12: Pass the garlic extract and turmeric extract through a 60-80 mesh sieve respectively;
[0026] S13: Pass vitamin C and zinc gluconate through a 100-120 mesh sieve respectively;
[0027] S14: Pass sodium selenite through a 120-140 mesh sieve;
[0028] S15: Pass the probiotic powder through a 60-80 mesh sieve at an ambient temperature ≤25℃.
[0029] Optionally, S2 specifically includes:
[0030] S21: Prepare microcrystalline cellulose as a carrier, the amount of which is 1-2 times the weight of artemisinin extract;
[0031] S22: Place the carrier in the mixer and start mixing at a speed of 100-200 rpm under nitrogen protection.
[0032] S23: Add the artemisinin extract to the mixer at a uniform speed and mix with the carrier for 10-20 minutes until a uniform premix A is formed.
[0033] Optionally, S3 specifically includes:
[0034] S31: Garlic extract and turmeric extract are added together into a mixing device with a jacketed heating function;
[0035] S32: Start the mixing equipment and mix at a stirring speed of 150-250 rpm, while raising the material temperature to 50-70℃. Continue mixing for 15-30 minutes to obtain a uniform premix B.
[0036] Optionally, S4 specifically includes:
[0037] S41: Inject purified water at a temperature of 30-40℃ into the chemical tank, the amount of which accounts for 10%-20% of the total weight of the eliminator;
[0038] S42: Add vitamin C, zinc gluconate and sodium selenite to purified water simultaneously while stirring at 300-500 rpm;
[0039] S43: Continue stirring until all components are completely dissolved, resulting in a clear solution C.
[0040] Optionally, S5 specifically includes:
[0041] S51: Add premix A and premix B to the mixer simultaneously;
[0042] S52: Mix at 20-35 rpm for 5-10 minutes to initially disperse the solid components evenly;
[0043] S53: The solution C is uniformly sprayed into the mixer at a flow rate of 1-2 L / min using a spray device, while the speed of the mixer is increased to 150-300 rpm.
[0044] S54: Maintain mixing speed for 20-40 minutes until the main mixture D is formed.
[0045] Optionally, S6 specifically includes:
[0046] S61: Transfer the main mixture D to a cooling mixing pot, and control the material temperature at 20-30℃ by circulating water in the jacket;
[0047] S62: Add the flavoring agent to the main mixture D and mix at 50-100 rpm for 3-5 minutes. The flavoring agent is natural peppermint oil or steviol glycosides.
[0048] S63: Add the probiotic powder at a uniform speed and mix at a speed of 50-100 rpm for 10-20 minutes to obtain the final mixture E.
[0049] Optionally, S7 specifically includes:
[0050] S71: Transfer the final mixture E to a fluidized bed dryer and dry it for 20-40 minutes at an inlet air temperature of 40-50℃, controlling the material moisture content to ≤5%;
[0051] S72: The dried material is granulated using a wet granulation machine, and 2-5% hydroxypropyl methylcellulose aqueous solution is sprayed simultaneously as a binder to obtain granules with a particle size of 0.5-1.0 mm.
[0052] S73: The granules are compressed into tablets at room temperature using a tablet press with a pressure of 15-25kN to obtain the finished expeller.
[0053] The beneficial effects of this invention are:
[0054] This invention uses artemisinin extract, garlic extract, and turmeric extract as the main active components against Toxoplasma gondii, combined with vitamin C, zinc gluconate, and sodium selenite to construct an immune-enhancing and antioxidant complex system, supplemented with probiotic powder to maintain intestinal flora balance, thereby forming a synergistic effect on the three levels of expelling parasites, regulating immunity, and repairing the body. This combination can effectively inhibit the reproduction and activity of Toxoplasma gondii in the host, reduce the risk of latent infection, improve the host's immune status, and enhance the body's ability to resist reinfection. Attached Figure Description
[0055] To more clearly illustrate the technical solutions in this invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only for this invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0056] Figure 1 This is a schematic diagram of the method for preparing the repellent according to an embodiment of the present invention. Detailed Implementation
[0057] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments. It should also be noted that, to make the embodiments more comprehensive, the following embodiments are the best and preferred embodiments, and those skilled in the art can use other alternative methods to implement some well-known technologies; moreover, the accompanying drawings are only for more specific description of the embodiments and are not intended to specifically limit the present invention.
[0058] It should be noted that the use of terms such as "an embodiment," "an embodiment," "an exemplary embodiment," and "some embodiments" in the specification indicates that the described embodiment may include a specific feature, structure, or characteristic, but not every embodiment necessarily includes that specific feature, structure, or characteristic. Furthermore, when a specific feature, structure, or characteristic is described in connection with an embodiment, implementing such a feature, structure, or characteristic in conjunction with other embodiments (whether explicitly described or not) should be within the knowledge of those skilled in the art.
[0059] Generally, terms can be understood at least partly from their use in context. For example, depending at least partly on the context, the term "one or more" as used herein can be used to describe any feature, structure, or characteristic in a singular sense, or a combination of features, structures, or characteristics in a plural sense. Additionally, the term "based on" can be understood not necessarily to convey an exclusive set of factors, but rather, alternatively, depending at least partly on the context, to allow for the presence of other factors that are not necessarily explicitly described.
[0060] Example 1
[0061] A latent Toxoplasma gondii expellant, comprising the following percentages: artemisinin extract 7%, garlic extract 13%, turmeric extract 7%, vitamin C 17%, zinc gluconate 7%, sodium selenite 2%, probiotic powder 40%, and flavoring agent 1%.
[0062] like Figure 1 As shown, a method for preparing a latent Toxoplasma gondii repellent includes the following steps:
[0063] S1: Artemisinin extract, garlic extract, turmeric extract, vitamin C, zinc gluconate, sodium selenite, and probiotic powder were sieved separately.
[0064] S2: Artemisinin extract is mixed with the prepared carrier to form premix A;
[0065] S3: Mix garlic extract and turmeric extract to prepare premix B;
[0066] S4: Dissolve vitamin C, zinc gluconate, and sodium selenite in purified water to prepare solution C;
[0067] S5: Mix premix A, premix B and solution C to form a homogeneous main mixture D;
[0068] S6: Add the probiotic powder and flavoring agent to the main mixture D, mix well, and obtain the final mixture E;
[0069] S7: The final mixture E is dried, granulated and shaped to obtain the repellent product.
[0070] S1 specifically includes:
[0071] S11: Artemisinin extract is passed through a 90-mesh sieve under an ambient humidity of 40%.
[0072] S12: Pass the garlic extract and turmeric extract through a 70-mesh sieve respectively;
[0073] S13: Pass vitamin C and zinc gluconate through a 110-mesh sieve respectively;
[0074] S14: Pass sodium selenite through a 130-mesh sieve;
[0075] S15: Pass the probiotic powder through a 70-mesh sieve at an ambient temperature of 20°C.
[0076] S2 specifically includes:
[0077] S21: Prepare microcrystalline cellulose as a carrier, the amount of which is 1.5 times the weight of artemisinin extract;
[0078] S22: Place the carrier in the mixer and start mixing at 150 rpm under nitrogen protection.
[0079] S23: Add artemisinin extract to the mixer at a uniform speed and mix with the carrier for 15 minutes until a uniform premix A is formed.
[0080] S3 specifically includes:
[0081] S31: Garlic extract and turmeric extract are added together into a mixing device with a jacketed heating function;
[0082] S32: Start the mixing equipment and mix at a stirring speed of 200 rpm. At the same time, heat the mixing equipment through the jacket to raise the material temperature to 60°C. Continue mixing for 20 minutes to obtain a uniform premix B.
[0083] S4 specifically includes:
[0084] S41: Pour purified water at a temperature of 35°C into the chemical tank, the amount of which accounts for 15% of the total weight of the eliminator;
[0085] S42: Add vitamin C, zinc gluconate and sodium selenite to purified water simultaneously while stirring at 400 rpm;
[0086] S43: Continue stirring until all components are completely dissolved, resulting in a clear solution C.
[0087] S5 specifically includes:
[0088] S51: Add premix A and premix B to the mixer simultaneously;
[0089] S52: Mix at 30 rpm for 8 minutes to initially disperse the solid components evenly;
[0090] S53: The solution C is uniformly sprayed into the mixer at a flow rate of 1.5L / min using a spray device, while the mixer speed is increased to 250rpm.
[0091] S54: Maintain mixing speed for 30 minutes until a wet primary mixture D with uniform color and texture is formed.
[0092] S6 specifically includes:
[0093] S61: Transfer the main mixture D to a cooling mixing pot, and control the material temperature at 25°C by circulating water through the jacket.
[0094] S62: Add the flavoring agent to the main mixture D and mix at 80 rpm for 4 minutes. The flavoring agent is steviol glycosides.
[0095] S63: Add the probiotic powder at a uniform speed and mix at 80 rpm for 15 minutes. When the mixture reaches a uniform state, the final mixture E is obtained.
[0096] S7 specifically includes:
[0097] S71: Transfer the final mixture E to a fluidized bed dryer and dry it for 30 minutes at an inlet air temperature of 45°C, controlling the material moisture content to 3%;
[0098] S72: The dried material is granulated using a wet granulation machine, and 3% hydroxypropyl methylcellulose aqueous solution is sprayed simultaneously as a binder to obtain granules with a particle size of 0.8 mm.
[0099] S73: The granules are compressed into tablets at room temperature using a tablet press with a pressure of 20kN to obtain the finished expeller.
[0100] Example 2
[0101] Formula composition: 5% artemisinin extract, 10% garlic extract, 5% turmeric extract, 15% vitamin C, 5% zinc gluconate, 1% sodium selenite, 58.5% probiotic powder, and 0.5% flavoring agent (natural peppermint oil).
[0102] The preparation steps are as follows:
[0103] S1: Artemisinin extract was passed through an 80-mesh sieve at an ambient humidity of 43%, garlic extract and turmeric extract were passed through a 60-mesh sieve, vitamin C and zinc gluconate were passed through a 100-mesh sieve, sodium selenite was passed through a 120-mesh sieve, and probiotic powder was passed through a 60-mesh sieve at an ambient temperature of 23°C to obtain uniform fine powder raw materials for later use.
[0104] S2: Take microcrystalline cellulose as a carrier, the amount of carrier is 1 times the weight of artemisinin extract, put it into a mixer, start mixing at 100 rpm under nitrogen protection, then add artemisinin extract at a uniform speed, maintain mixing for 10 minutes to form a uniform premix A.
[0105] S3: Add garlic extract and turmeric extract together into a mixing device with jacketed heating function, set the stirring speed to 150 rpm, and heat to 50°C. Maintain this temperature and continue mixing for 15 minutes to fully blend the two extracts and obtain premix B.
[0106] S4: Add purified water at 30°C to the chemical tank. The amount of purified water accounts for 10% of the total weight of the eliminator. Add vitamin C, zinc gluconate and sodium selenite to the purified water in sequence at a stirring speed of 300 rpm. Continue stirring until completely dissolved to obtain a clear and transparent solution C.
[0107] S5: Add premix A and premix B into the mixer at the same time and mix at 20 rpm for 5 minutes to achieve initial uniform dispersion. Then, spray solution C evenly through the spray device at a flow rate of 1 L / min, and increase the mixer speed to 150 rpm and continue mixing for 20 minutes to form a main mixture D with uniform color and texture.
[0108] S6: Transfer the main mixture D to the cooling mixing pot, control the material temperature at 20°C by circulating water in the jacket, then add natural peppermint oil flavoring, mix at 50 rpm for 4 minutes, then add probiotic powder at a uniform speed, mix at 50 rpm for 10 minutes to obtain a uniformly distributed final mixture E.
[0109] S7: Transfer the final mixture E into a fluidized bed dryer and dry it for 20 minutes at an inlet air temperature of 40°C, controlling the material moisture content to 5%. After drying, granulate it using a wet granulator, simultaneously spraying 2% hydroxypropyl methylcellulose aqueous solution as a binder to obtain granules with a particle size of 0.5 mm. Finally, press it into tablets at room temperature under a pressure of 15 kN using a tableting machine to obtain the finished expellant.
[0110] Example 3
[0111] Formula composition: 10% artemisinin extract, 15% garlic extract, 10% turmeric extract, 20% vitamin C, 10% zinc gluconate, 3% sodium selenite, 30% probiotic powder, and 2% flavoring agent (steviosides).
[0112] The preparation steps are as follows:
[0113] S1: Artemisinin extract was passed through a 100-mesh sieve at an ambient humidity of 45%, garlic extract and turmeric extract were passed through an 80-mesh sieve, vitamin C and zinc gluconate were passed through a 120-mesh sieve, sodium selenite was passed through a 140-mesh sieve, and probiotic powder was passed through an 80-mesh sieve at an ambient temperature of 25℃ to obtain uniform fine powder raw materials for later use.
[0114] S2: Take microcrystalline cellulose as a carrier, the amount of carrier is twice the weight of artemisinin extract, put it into a mixer, start mixing at 200 rpm under nitrogen protection, then add artemisinin extract at a uniform speed, maintain mixing for 20 minutes to form a uniform premix A.
[0115] S3: Add garlic extract and turmeric extract together into a mixing device with jacketed heating function, set the stirring speed to 250 rpm, and heat to 70°C. Maintain this temperature and continue mixing for 30 minutes to fully blend the two extracts and obtain premix B.
[0116] S4: Add purified water at 40°C to the chemical tank. The amount of purified water accounts for 20% of the total weight of the eliminator. Add vitamin C, zinc gluconate and sodium selenite to the purified water in sequence at a stirring speed of 500 rpm. Continue stirring until completely dissolved to obtain a clear and transparent solution C.
[0117] S5: Add premix A and premix B into the mixer at the same time and mix at 35 rpm for 10 minutes to achieve initial uniform dispersion. Then, spray solution C evenly through the spray device at a flow rate of 2 L / min, and increase the mixer speed to 300 rpm and continue mixing for 40 minutes to form a main mixture D with uniform color and texture.
[0118] S6: Transfer the main mixture D to the cooling mixing pot, control the material temperature at 30°C by circulating water in the jacket, then add the steviol glycoside flavoring agent, mix at 100 rpm for 5 minutes, then add the probiotic powder at a uniform speed, and mix at 100 rpm for 20 minutes to obtain the uniformly distributed final mixture E.
[0119] S7: Transfer the final mixture E into a fluidized bed dryer and dry it for 40 minutes at an inlet air temperature of 50°C, controlling the material moisture content to 4%. After drying, granulate it using a wet granulator, and simultaneously spray 5% hydroxypropyl methylcellulose aqueous solution as a binder to obtain granules with a particle size of 1mm. Finally, press it into tablets at room temperature and a pressure of 25kN through a tableting machine to obtain the expellant product.
[0120] To verify the actual effect of the preparation method of the latent Toxoplasma gondii expellant described in this invention on the quality of the finished product, the finished products obtained in Examples 1, 2 and 3 were selected for comparative testing. The test items included: moisture content, particle size distribution uniformity, tablet hardness, disintegration time, retention rate of active ingredient content, uniformity of artemisinin content, probiotic survival rate, antioxidant retention rate and in vitro anti-Toxoplasma gondii inhibition rate, a total of 9 items. The test data for each item are shown in Table 1.
[0121] Table 1 Comparison of Finished Product Performance Parameters
[0122]
[0123]
[0124] As shown in Table 1, Example 1, employing an optimized drying temperature and moisture control strategy, resulted in a finished tablet moisture content of 3.0%, significantly lower than that of Example 2 (5.0%) and Example 3 (4.0%), effectively reducing the risk of moisture absorption, softening, and component degradation. Example 1 utilized a spray rate of 1.5 L / min and a rotation speed of 250 rpm during the spray mixing stage, ensuring thorough distribution of components in the wet mixture. The standard deviation of particle size was controlled within 0.03 mm, and the artemisinin content RSD was 1.8%, both superior to the control group, indicating more stable component distribution. Example 1 achieved a tablet hardness of 85 N and exhibited good disintegration resistance. The dissolution time is only 8 minutes, indicating that it has both good mechanical strength and ensures rapid release, meeting the dual requirements of administration and dissolution. After 30 days of accelerated stability testing at 40℃ / 75%RH, the effective ingredient retention rate of Example 1 still reached 96.5%, and the probiotic survival rate reached 94.2%, which are 6.7% and 7.2% higher than those of Example 2, respectively, indicating that it has stronger thermal stability and antioxidant properties. In the in vitro culture experiment, the 72-hour inhibition rate of Toxoplasma gondii trophozoites reached 93.4%, which is significantly improved compared with Example 2 (85.7%) and Example 3 (89.5%), indicating that its formulation has the best synergistic effect.
[0125] In summary, the combination of ingredients and process parameters used in Example 1 is the most reasonable, which can significantly improve the uniformity, stability, biological activity retention, and final anti-Toxoplasma gondii efficacy of the expellant. Therefore, it is the preferred embodiment of the present invention.
[0126] This invention encompasses any substitutions, modifications, equivalent methods, and solutions made within the spirit and scope of this invention. To provide the public with a thorough understanding of this invention, specific details are described in detail in the following preferred embodiments; however, those skilled in the art will fully understand the invention even without these details. Furthermore, to avoid unnecessary misunderstanding of the essence of this invention, well-known methods, processes, procedures, components, and circuits are not described in detail.
[0127] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.
Claims
1. A repellent for latent Toxoplasma gondii infection, characterized in that, It includes artemisinin extract, garlic extract, turmeric extract, vitamin C, zinc gluconate, sodium selenite, probiotic powder, and flavoring agents; the percentage of each component by mass is as follows: Artemisinin extract accounts for 5-10%; Garlic extract accounts for 10-15%; Turmeric extract accounts for 5-10%; Vitamin C accounts for 15-20%; Zinc gluconate accounts for 5-10%; Sodium selenite accounts for 1-3%; Probiotic powder accounts for 30-58.5%; Flavoring agent content: 0.5-2%.
2. The toxoplasmosis repellent according to claim 1, characterized in that, The percentages of each component are as follows: artemisinin extract 7%, garlic extract 13%, turmeric extract 7%, vitamin C 17%, zinc gluconate 7%, sodium selenite 2%, probiotic powder 40%, and flavoring agent 1%.
3. A method for preparing a latent Toxoplasma gondii repellent, used to prepare the latent Toxoplasma gondii repellent according to any one of claims 1-2, characterized in that, Includes the following steps: S1: Artemisinin extract, garlic extract, turmeric extract, vitamin C, zinc gluconate, sodium selenite, and probiotic powder were sieved separately. S2: Artemisinin extract is mixed with the prepared carrier to form premix A; S3: Mix garlic extract and turmeric extract to prepare premix B; S4: Dissolve vitamin C, zinc gluconate, and sodium selenite in purified water to prepare solution C; S5: Mix premix A, premix B and solution C to form a homogeneous main mixture D; S6: Add the probiotic powder and flavoring agent to the main mixture D, mix well, and obtain the final mixture E; S7: The final mixture E is dried, granulated and shaped to obtain the repellent product.
4. The method for preparing a latent Toxoplasma gondii repellent according to claim 3, characterized in that, S1 specifically includes: S11: Artemisinin extract is passed through an 80-100 mesh sieve under ambient humidity ≤45%; S12: Pass the garlic extract and turmeric extract through a 60-80 mesh sieve respectively; S13: Pass vitamin C and zinc gluconate through a 100-120 mesh sieve respectively; S14: Pass sodium selenite through a 120-140 mesh sieve; S15: Pass the probiotic powder through a 60-80 mesh sieve at an ambient temperature ≤25℃.
5. The method for preparing a latent Toxoplasma gondii repellent according to claim 3, characterized in that, S2 specifically includes: S21: Prepare microcrystalline cellulose as a carrier, the amount of which is 1-2 times the weight of artemisinin extract; S22: Place the carrier in the mixer and start mixing at a speed of 100-200 rpm under nitrogen protection. S23: Add the artemisinin extract to the mixer at a uniform speed and mix with the carrier for 10-20 minutes until a uniform premix A is formed.
6. The method for preparing a latent Toxoplasma gondii repellent according to claim 3, characterized in that, S3 specifically includes: S31: Garlic extract and turmeric extract are added together into a mixing device with a jacketed heating function; S32: Start the mixing equipment and mix at a stirring speed of 150-250 rpm, while raising the material temperature to 50-70℃. Continue mixing for 15-30 minutes to obtain a uniform premix B.
7. The method for preparing a latent Toxoplasma gondii repellent according to claim 3, characterized in that, S4 specifically includes: S41: Inject purified water at a temperature of 30-40℃ into the chemical tank, the amount of which accounts for 10%-20% of the total weight of the eliminator; S42: Add vitamin C, zinc gluconate and sodium selenite to purified water simultaneously while stirring at 300-500 rpm; S43: Continue stirring until all components are completely dissolved, resulting in a clear solution C.
8. The method for preparing a latent Toxoplasma gondii repellent according to claim 3, characterized in that, S5 specifically includes: S51: Add premix A and premix B to the mixer simultaneously; S52: Mix at 20-35 rpm for 5-10 minutes to initially disperse the solid components evenly; S53: The solution C is uniformly sprayed into the mixer at a flow rate of 1-2 L / min using a spray device, while the speed of the mixer is increased to 150-300 rpm. S54: Maintain mixing speed for 20-40 minutes until the main mixture D is formed.
9. The method for preparing a latent Toxoplasma gondii repellent according to claim 3, characterized in that, S6 specifically includes: S61: Transfer the main mixture D to a cooling mixing pot, and control the material temperature at 20-30℃ by circulating water in the jacket; S62: Add the flavoring agent to the main mixture D and mix at 50-100 rpm for 3-5 minutes. The flavoring agent is natural peppermint oil or steviol glycosides. S63: Add the probiotic powder at a uniform speed and mix at a speed of 50-100 rpm for 10-20 minutes to obtain the final mixture E.
10. The method for preparing a latent Toxoplasma gondii repellent according to claim 3, characterized in that, Specifically, S7 includes: S71: Transfer the final mixture E to a fluidized bed dryer and dry it for 20-40 minutes at an inlet air temperature of 40-50℃, controlling the material moisture content to ≤5%; S72: The dried material is granulated using a wet granulation machine, and 2-5% hydroxypropyl methylcellulose aqueous solution is sprayed simultaneously as a binder to obtain granules with a particle size of 0.5-1.0 mm. S73: The granules are compressed into tablets at room temperature using a tablet press with a pressure of 15-25kN to obtain the finished expeller.