An in vitro diagnostic kit and method for an embedded fluorescent labeling detection system

By employing precise optical design, sophisticated fluid control, and adaptive signal processing in the embedded fluorescent labeling detection system, the problems of optical interference and fluid pulsation were solved, enabling efficient physical separation and quantitative detection of multiple fluorescent labels, thus improving detection specificity and reliability.

CN121324322BActive Publication Date: 2026-05-26CHENYANG ZHENGLIN TECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHENYANG ZHENGLIN TECHNOLOGY CO LTD
Filing Date
2025-10-29
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

Existing embedded fluorescent labeling detection systems face severe optical signal crosstalk, significant background noise interference, and insufficient fluid control precision when performing high-throughput multi-index joint detection, leading to decreased detection specificity and increased risk of false positives.

Method used

A micro-spectral filter assembly consisting of an excitation source array with precise wavelength alignment and eight dielectric films, combined with a 52° angled optical path design and a band-stop filter, achieves efficient physical separation of the excitation and emission spectra of multiple fluorescent markers. The fluid control module achieves sub-microliter precision fluid transmission through a micro-valve array driven by a thermosensitive shape memory alloy, a pulsation damper for the coupled air chamber, and a high-frequency sampling pressure feedback loop. The signal processing module constructs a crosstalk matrix using an adaptive spectral deconvolution algorithm and applies non-negative least squares method for mathematical separation.

Benefits of technology

It effectively overcomes signal crosstalk, improves the specificity and accuracy of multi-index joint detection, ensures the uniformity of the reaction system and the repeatability and reliability of the detection results, and improves the detection signal-to-noise ratio by two orders of magnitude.

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Abstract

This invention belongs to the field of fluorescent labeling detection technology and discloses an in vitro diagnostic kit and method for an embedded fluorescent labeling detection system. The kit includes a microfluidic chip carrier, an optical detection module, a fluid control module, and a signal processing module. The optical detection module employs a wavelength-precisely aligned excitation source array and narrowband spectral filtering components, combined with a specific angled optical path design, to physically suppress optical crosstalk and background noise. The fluid control module achieves high-precision and stable control of micro-volume fluids through the synergy of a micro-valve array, a pulsation damper, and a high-frequency pressure feedback loop. This invention fundamentally solves the problems of crosstalk, noise, and fluid control accuracy in multi-label fluorescence detection through triple innovation of hardware optical path purification, precise fluid control, and software algorithm decoupling, significantly improving the specificity, accuracy, and reliability of the detection.
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Citation Information

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