Preparation device and method of silybum marianum phospholipid complex

Abstract

The invention relates to the field of traditional Chinese medicine synthesis and preparation, in particular to a preparation device and method of a silybum marianum phospholipid complex. According to the preparation method of the silybum marianum phospholipid complex, the preparation device of the silybum marianum phospholipid complex is used, the steps of compounding treatment, filtering and drying are optimized, the compounding rate of the prepared silybum marianum phospholipid complex is increased, and the yield of the silybum marianum phospholipid complex is improved. The prepared silybum marianum phospholipid complex product has the advantages of low solvent residue and good water solubility and fat solubility, in addition, on the basis of completing two different filtering treatment in sequence, the silybum marianum phospholipid complex can be rapidly switched to a drying treatment mode after completing the second filtering treatment, the steps of the silybum marianum phospholipid complex preparation treatment process are reduced, and the production cost is reduced. The preparation process is simple, low in cost and suitable for industrial production, the technical defects that the preparation steps of the silybum marianum phospholipid complex are complex and tedious, and the preparation treatment efficiency is poor are greatly overcome, and the preparation work efficiency of the silybum marianum phospholipid complex is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of Chinese medicine synthesis, and particularly relates to a preparation device and method of a silymarin phospholipid complex. BACKGROUND

[0002] Silymarin has various pharmacological activities, such as liver protection, anti-inflammatory, antioxidant and the like, but its poor water solubility and low bioavailability limit its clinical application. In order to improve its solubility and bioavailability, researchers have developed various technologies, among which the phospholipid complex is an important method. In addition, although the silymarin phospholipid complex has significant advantages in improving the bioavailability of drugs, the preparation process still has problems such as complex steps and many types of equipment, for example, multiple filtration and drying processes are required in sequence, and the complex solvent needs to be frequently transferred between multiple devices, resulting in complex and tedious preparation steps of the silymarin phospholipid complex, and poor preparation efficiency. SUMMARY

[0003] In order to overcome the shortcomings of complex and tedious preparation steps of the silymarin phospholipid complex and poor preparation efficiency, the present application provides a preparation device and method of a silymarin phospholipid complex.

[0004] Technical scheme: A preparation device of a silymarin phospholipid complex comprises a supporting base frame, an electric rotating table, a rotating frame, a filter cabin, a tee joint pipe, a liquid inlet guide pipe, a hollow cylinder, a rotating motor and rotating strips; the supporting base frame is provided with the electric rotating table; the rotating part of the electric rotating table is fixedly connected with the rotating frame; the rotating frame is provided with the filter cabin; the lower side of the filter cabin is provided with a connecting channel structure; the bottom of the filter cabin is provided with a plurality of bottom filter hole structures connected with the connecting channel; the inner wall of the filter cabin is provided with a plurality of side filter hole structures connected with the connecting channel; the top of the filter cabin is detachably fixed with the tee joint pipe, and the middle part of the tee joint pipe is provided with a side channel structure; the tee joint pipe is fixedly connected with the liquid inlet guide pipe; the bottom of the filter cabin is rotatably connected with the hollow cylinder, and the hollow cylinder is provided with a plurality of liquid outlet grooves; the lower end of the liquid inlet guide pipe is rotatably connected with the hollow cylinder; the filter cabin is provided with the rotating motor for driving the hollow cylinder to rotate; the output shaft of the rotating motor is fixedly connected with the hollow cylinder; and the hollow cylinder is fixedly connected with not less than two rotating strips close to the bottom of the filter cabin.

[0005] Preferably, the bottom filter hole is an inclined structure facing the inner wall of the filter cabin.

[0006] Preferably, the inner wall of the filter cabin is provided with a plurality of spiral flow guide strip structures.

[0007] Preferably, the bottom of the rotating strip is provided with a plurality of through grooves penetrating in the rotating direction.

[0008] Preferably, the lower end of the liquid inlet pipe is fixed with a coarse filter cylinder, and the coarse filter cylinder is initially located in the hollow cylinder.

[0009] Preferably, the liquid inlet pipe is detachably fixed with the top plug.

[0010] Preferably, a filter basket is slidably connected to the liquid inlet pipe; and an electric push rod is installed on the liquid inlet pipe to push the filter basket to move up and down.

[0011] Preferably, a humidity sensor for continuously monitoring the gas flow rate is installed on the side channel of the three-way pipe.

[0012] A preparation method of a silybum marianum phospholipid complex includes the following steps: Step one, mix HPLC 80% silybum marianum extract and PC50-PC90 phospholipid to form a mixed solution, pour the mixed solution into a filter cabin, and the mass ratio of HPLC 80% silybum marianum extract to PC50-PC90 phospholipid is 1:0.5-1:2; Step two, concentrate the mixed solution under reduced pressure to obtain a first silybum marianum phospholipid complex solvent, and filter the first silybum marianum phospholipid complex solvent through the bottom filter hole structure and the side filter hole structure of the filter cabin, and recover the filtrate; Step three, mix the recovered filtrate with n-hexane, dichloromethane and petroleum ether to obtain a second silybum marianum phospholipid complex solvent, and pour the second silybum marianum phospholipid complex solvent into the filter cabin; Step four, filter the second silybum marianum phospholipid complex solvent through the bottom filter hole structure and the side filter hole structure of the filter cabin, and dry the filter residue to obtain precipitated particles, which are high-complexity, high-drug-content silybum marianum phospholipid complexes.

[0013] Beneficial effects: the preparation method of the silybum marianum phospholipid complex optimizes the steps of complex processing, filtering and drying, improves the complex rate of the prepared silybum marianum phospholipid complex, and the prepared silybum marianum phospholipid complex product has the advantages of low solvent residue, good water solubility and good fat solubility. In addition, on the basis of completing two different filtering processes in sequence, the drying process mode can be quickly switched after completing the second filtering process, which reduces the preparation process steps of the silybum marianum phospholipid complex, simplifies the preparation process, reduces the cost, is suitable for industrial production, greatly improves the complex rate of the silybum marianum phospholipid complex, and improves the preparation efficiency of the silybum marianum phospholipid complex. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 It is a perspective view of a silybum marianum phospholipid complex preparation device; Figure 2 is a stereoscopic sectional view of a filter cabin of a preparation device for a silybum marianum phospholipid complex; Figure 3 is a stereoscopic sectional view of a filter cabin and a tee pipe of a preparation device for a silybum marianum phospholipid complex; Figure 4 is a stereoscopic sectional view of a filter cabin and a tee pipe of a preparation device for a silybum marianum phospholipid complex; Figure 3 is a stereoscopic enlarged view of H area of the preparation device for a silybum marianum phospholipid complex; Figure 5 is a stereoscopic view of a liquid inlet guide pipe of a preparation device for a silybum marianum phospholipid complex; Figure 6 is a stereoscopic view of a scraping strip of a preparation device for a silybum marianum phospholipid complex.

[0015] Markings in the drawings: 11 - supporting chassis, 12 - electric rotary table, 13 - rotary frame, 2 - filter cabin, 201 - connecting passage, 202 - bottom filter hole, 203 - side filter hole, 204 - spiral flow guide strip, 31 - tee pipe, 3101 - side passage, 32 - liquid inlet guide pipe, 33 - hollow cylinder, 3301 - liquid outlet slot, 34 - rotary motor, 35 - rotary strip, 3501 - through slot, 36 - coarse filter cylinder, 37 - top plug, 38 - filter hopper, 39 - electric push rod, 4 - humidity sensor. DETAILED DESCRIPTION

[0016] The following description is merely exemplary in nature and is in no way intended to limit the scope of the application, as described.

[0017] Example 1, a preparation device for a silybum marianum phospholipid complex, as shown in Figures 1-6As shown, it comprises a supporting chassis 11, an electric rotating table 12, a rotating frame 13, a filter cabin 2, a three-way pipe 31, a liquid inlet guide pipe 32, a hollow cylinder 33, a rotating motor 34 and rotating strips 35; the supporting chassis 11 is provided with the electric rotating table 12; the rotating part of the electric rotating table 12 is fixedly connected with the rotating frame 13; the rotating frame 13 is provided with the filter cabin 2; the lower side of the filter cabin 2 is provided with a connecting channel 201 structure; the inner bottom of the filter cabin 2 is provided with a plurality of bottom filter holes 202 structures connected with the connecting channel 201, and the bottom filter holes 202 are inclined structures facing the inner wall of the filter cabin 2; the inner wall of the filter cabin 2 is provided with a plurality of side filter holes 203 structures connected with the connecting channel 201; the inner wall of the filter cabin 2 is provided with a plurality of spiral flow guide strips 204 structures; the top of the filter cabin 2 is detachably fixed with the three-way pipe 31 through a threaded structure, and the middle part of the three-way pipe 31 is provided with a side channel 3101 structure; the upper inlet end of the three-way pipe 31 is fixedly connected with the liquid inlet guide pipe 32, and the liquid inlet guide pipe 32 passes through the lower outlet end of the three-way pipe 31 downward; the inner bottom of the filter cabin 2 is rotatably connected with the hollow cylinder 33, and the hollow cylinder 33 is provided with a plurality of liquid outlet grooves 3301 structures; the lower end of the liquid inlet guide pipe 32 is rotatably connected with the hollow cylinder 33; the filter cabin 2 is provided with the rotating motor 34 for driving the hollow cylinder 33 to rotate; the output shaft of the rotating motor 34 is fixedly connected with the hollow cylinder 33; the hollow cylinder 33 is fixedly connected with two rotating strips 35, and the rotating strips 35 are in close contact with the inner bottom of the filter cabin 2; the bottom of each of the two rotating strips 35 is provided with a plurality of penetration grooves 3501 structures penetrating in the rotating direction, and the width of the penetration grooves 3501 of the rotating strips 35 is greater than the width of the bottom filter holes 202 of the filter cabin 2.

[0018] As shown in Figure 3 and Figure 5 As shown, the lower outlet end of the liquid inlet guide pipe 32 is fixedly connected with a coarse filter cylinder 36, and the coarse filter cylinder 36 is initially located in the hollow cylinder 33; the top of the liquid inlet guide pipe 32 is detachably fixed with a top plug 37 through a threaded structure.

[0019] A preparation method of a water flyweed phospholipid compound, comprising the following steps: Step one, mix HPLC 80% water flyweed extract, PC50-PC90 phospholipid, 95% ethanol, ethyl acetate and acetone to form a mixed solution into the filter cabin 2, wherein the mass ratio of HPLC 80% water flyweed extract and PC50-PC90 phospholipid is 1:0.5-1:2; Step two, after the mixed solution is reduced pressure concentrated, a first water flyweed phospholipid compound solvent is obtained, and the first water flyweed phospholipid compound solvent is filtered through the bottom filter hole 202 structure and the side filter hole 203 structure of the filter cabin 2, and the filtrate is recovered; Step three, mix the recovered filtrate with n-hexane, dichloromethane and petroleum ether to obtain a second water flyweed phospholipid compound solvent, and pour it into the filter cabin 2. Step four, the second portion of the water flygong phospholipid complex solvent is filtered through the bottom filter hole 202 structure and the side filter hole 203 structure of the filter cabin 2, and the filter residue is dried to obtain a precipitated particle, which is a high-complexity, high-drug-content water flygong phospholipid complex, and the prepared water flygong phospholipid complex product has the advantages of low solvent residue, good water solubility and good fat solubility.

[0020] The use method of the water flygong phospholipid complex preparation device of the present application is as follows.

[0021] First, open the top plug 37 on the filter 38, connect the side channel 3101 of the tee pipe 31 to the pressurized air pump, connect the connecting channel 201 of the filter cabin 2 to the filtrate collection container, continuously inject the first portion of the water flygong phospholipid complex solvent into the filter cabin 2 through the liquid inlet guide pipe 32 and the liquid outlet channel 3301 of the hollow cylinder 33, and then plug the top plug 37 back into the filter 38. The large volume impurities in the water flygong phospholipid complex solvent will be intercepted by the coarse filter cylinder 36 in the hollow cylinder 33 and cannot enter the filter cabin 2. After the water flygong phospholipid complex solvent enters the filter cabin 2, it will be filtered through the bottom filter hole 202 and the side filter hole 203. At the same time, the pressurized air pump connected externally continuously sends pressurized air flow into the filter cabin 2 through the side channel 3101 of the tee pipe 31. After the pressurized air flow enters the filter cabin 2, it will exert a squeezing force on the water flygong phospholipid complex solvent, increasing the speed of the water flygong phospholipid complex solvent passing through the bottom filter hole 202 and the side filter hole 203 for filtering treatment. The filtrate obtained by filtering flows through the connecting channel 201 and is collected in the external filtrate collection container. The filter residue obtained by filtering remains in the filter cabin 2. After the filtering treatment of the first portion of the water flygong phospholipid complex solvent is completed, the external pressurized air pump and the external filtrate collection container are removed, and the rotary frame 13 and the filter cabin 2 are flipped one hundred and eighty degrees to the inverted state by the electric rotary table 12. The tee pipe 31, the liquid inlet guide pipe 32 and the coarse filter cylinder 36 are removed from the filter cabin 2. The filter residue in the filter cabin 2 can be poured out, the filter residue cleaning work of the first portion of the water flygong phospholipid complex solvent is completed, and finally the rotary frame 13 and the filter cabin 2 are flipped one hundred and eighty degrees in the opposite direction for resetting by the electric rotary table 12. The tee pipe 31, the liquid inlet guide pipe 32 and the coarse filter cylinder 36 are inserted back into the filter cabin 2.

[0022] Afterwards, the filtration treatment work of the second portion of the solvent of the silybum marianum phospholipids compound is completed according to the above steps, the filtrate obtained by filtration flows through the connecting channel 201 and is recovered into the external filtrate collection container, the filter residue obtained by filtration remains in the filter cabin 2, the filter residue is the silybum marianum phospholipids compound precipitation particles, after the filtration treatment work of the second portion of the solvent of the silybum marianum phospholipids compound is completed, the external pressurized gas pump and the external filtrate collection container are removed, the top plug 37 is in the state of being plugged into the filter hopper 38, and then the connecting channel 201 of the filter cabin 2 is externally connected to the hot gas flow conveying device, the hot gas flow is continuously conveyed from the external hot gas flow conveying device to the inside of the filter cabin 2 through the connecting channel 201 along the bottom filter hole 202 and the side filter hole 203, the hot gas flow flows out through the side channel 3101 of the three-way pipe 31, the hot gas flow blown out from the bottom towards the filter hole 202 of the inner side wall of the filter cabin 2 blows towards the inner side wall of the filter cabin 2, dries the inner wall of the filter cabin 2, avoids that the precipitation particles are adhered to the inner wall of the filter cabin 2 when the precipitation particles are discharged, and at the same time, the rotating motor 34 continuously drives the rotating strip 35 to slowly rotate along the bottom of the filter cabin 2, the width of the through slot 3501 of the rotating strip 35 is greater than the width of the bottom filter hole 202 of the filter cabin 2, when the through slot 3501 of the rotating strip 35 is aligned with the bottom filter hole 202, the hot gas flow blown out from the bottom filter hole 202 blows towards the bottom of the filter cabin 2 along the through slot 3501, the precipitation particles scraped off from the bottom of the filter cabin 2 are blown away, the precipitation particles are continuously tumbled under the blowing of the hot gas flow, the continuously tumbled precipitation particles are continuously dried by the hot gas flow, the hot gas flow blown towards the inner side wall of the filter cabin 2 flows in a spiral upward state along the spiral flow guide strip 204, the residence time of the hot gas flow in the filter cabin 2 is prolonged, and the drying treatment speed and uniformity of the precipitation particles in the filter cabin 2 are improved, after the drying treatment of the precipitation particles is completed, the external hot gas flow conveying device is removed, the rotating frame 13 and the filter cabin 2 are flipped by one hundred and eighty degrees to the inverted state by the electric rotating table 12, and the three-way pipe 31, the liquid inlet guide pipe 32 and the coarse filter cylinder 36 are taken out of the filter cabin 2, so that the precipitation particles in the filter cabin 2 that have completed the drying treatment can be poured out, the filtration treatment work of the second portion of the solvent of the silybum marianum phospholipids compound and the drying treatment work of the precipitation particles are carried out in the same device, finally, the rotating frame 13 and the filter cabin 2 are flipped by one hundred and eighty degrees in the reverse direction for resetting by the electric rotating table 12, and the three-way pipe 31, the liquid inlet guide pipe 32 and the coarse filter cylinder 36 are inserted back into the filter cabin 2, the preparation process steps of the silybum marianum phospholipids compound are reduced, the preparation process is simple, the cost is low, and it is suitable for industrial production.

[0023] Example 2, as Figures 1-6As shown, the filter 38 is initially inserted between the outer surface of the liquid inlet pipe 32 and below the tee pipe 31. The electric push rod 39 is installed on the liquid inlet pipe 32. The telescopic end of the electric push rod 39 is fixedly connected with the filter 38. When the liquid in the filter cabin 2 is subjected to pressure filtration treatment, the electric push rod 39 pulls the filter 38 to move downward along the liquid inlet pipe 32 away from the tee pipe 31, so that the pressurized air flow entering the side channel 3101 can smoothly enter the filter cabin 2 through the tee pipe 31, reducing the flow rate loss of the pressurized air flow caused by the obstruction of the filter 38, and making the gas flow into the filter cabin 2 faster. In the subsequent drying process of the precipitated particles in the filter cabin 2, the electric push rod 39 pushes the filter 38 to re-insert into the tee pipe 31 along the liquid inlet pipe 32. When the hot air flow passes through the tee pipe 31 and exits from the side channel 3101, as the precipitated particles are continuously dried and dehydrated, the weight of the precipitated particles gradually decreases. When it is necessary to improve the drying treatment efficiency and effect of the precipitated particles, it is necessary to increase the flow rate of the hot air flow blown into the filter cabin 2. The rotating strip 35 will crush part of the precipitated particles gathered in a lump shape at the bottom of the filter cabin 2 during rotation. At this time, part of the light-weight precipitated particle powder that has completed the drying treatment will be blown upward by the hot air flow flowing through the tee pipe 31. The filter 38 located in the tee pipe 31 intercepts the light-weight precipitated particles carried by the hot air flow, avoiding the light-weight precipitated particles from following the hot air flow to pass through the tee pipe 31 and exit from the side channel 3101, thereby reducing the waste of precipitated particle products.

[0024] As shown in the drawings, Figure 3 The humidity sensor 4 is installed on the side channel 3101 of the tee pipe 31. During the drying process of the precipitated particles in the filter cabin 2, the humidity sensor 4 continuously monitors the moisture content of the hot air flow flowing through the side channel 3101, and observes whether the precipitated particles in the filter cabin 2 meet the specified drying standard.

[0025] The above is only a specific embodiment of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art can easily think of changes or replacements within the technical range disclosed by the present application, which shall be covered within the protection scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.

Claims

1. A device for preparing a silybum marianum phospholipid complex, comprising a support chassis (11); characterized in that It also comprises an electric rotating table (12);The electric rotating table (12) is installed on the support chassis (11);The rotating part of the electric rotating table (12) is fixedly connected with a rotating frame (13);The rotating frame (13) is installed in the filter cabin (2);The lower side of the filter cabin (2) is provided with a connecting channel (201) structure;A plurality of bottom filter holes (202) are formed in the bottom of the filter cabin (2) and connected with the connecting channel (201);A plurality of side filter holes (203) are formed in the inner wall of the filter cabin (2) and connected with the connecting channel (201);A three-way pipe (31) is detachably fixed on the top of the filter cabin (2), and a side channel (3101) structure is formed in the middle of the three-way pipe (31);An inlet guide pipe (32) is fixedly connected with the three-way pipe (31);A hollow cylinder (33) is rotatably connected with the bottom of the filter cabin (2), and a plurality of liquid outlet grooves (3301) are formed in the hollow cylinder (33);The lower end of the inlet guide pipe (32) is rotatably connected with the hollow cylinder (33);A rotating motor (34) is installed on the filter cabin (2) to drive the hollow cylinder (33) to rotate;The output shaft of the rotating motor (34) is fixedly connected with the hollow cylinder (33);Not less than two rotating strips (35) are fixedly connected with the hollow cylinder (33) and closely contact with the bottom of the filter cabin (2).

2. The device for preparing the phopholipid complex of S. marina according to claim 1, characterized in that, The bottom filter hole (202) is inclined towards the inner wall of the filter cabin (2).

3. The device for preparing the phopholipid complex of S. marina according to claim 1, characterized in that, The inner wall of the filter cabin (2) is provided with a plurality of spiral guide strips (204).

4. The device for preparing the phopholipid complex of S. marina according to claim 1, characterized in that, A plurality of through grooves (3501) are formed in the bottom of the rotating strip (35) and penetrate in the rotating direction.

5. The device for preparing the phopholipid complex of S. marina according to claim 1, characterized in that, A coarse filter cylinder (36) is fixedly connected with the lower end of the inlet guide pipe (32), and the coarse filter cylinder (36) is initially located in the hollow cylinder (33).

6. The device for preparing the phopholipid complex of S. marina according to claim 1, characterized in that, A top plug (37) is detachably fixed on the inlet guide pipe (32).

7. The device for preparing the phopholipid complex of S. marina according to claim 1, characterized in that, A filter basket (38) is slidably connected with the inlet guide pipe (32);An electric push rod (39) is installed on the inlet guide pipe (32) to drive the filter basket (38) to move up and down.

8. The device for preparing the phopholipid complex of S. marina according to claim 7, characterized in that, A humidity sensor (4) is installed on the side channel (3101) of the three-way pipe (31) to continuously monitor the gas flow rate.

9. A method for preparing a silybin-phospholipid complex, using the device for preparing a silybin-phospholipid complex according to any one of claims 1 to 8, characterized in that, It comprises the following steps: Step one, mix HPLC 80% silybum marianum extract, PC50-PC90 phospholipid, 95% ethanol, ethyl acetate and acetone to form a mixed solution, and pour the mixed solution into the filter cabin (2); Step two, after reducing pressure concentration, the first silybum marianum phospholipid complex solvent is obtained, and the first silybum marianum phospholipid complex solvent is filtered through the bottom filter hole (202) structure and the side filter hole (203) structure of the filter cabin (2), and the filtrate is recovered; Step three, pour the second silybum marianum phospholipid complex solvent obtained by mixing the recovered filtrate with n-hexane, dichloromethane and petroleum ether into the filter cabin (2); Step four, the second water fly gourd phospholipid complex solvent is filtered through the bottom filter hole (202) structure and the side filter hole (203) structure of the filter cabin (2), and the filter residue is dried to obtain precipitated particles, which are high-complexity, high-drug-content water fly gourd phospholipid complexes.

10. The method of claim 9, wherein the water fly alga phospholipid complex is prepared by the steps of: The mass ratio of HPLC 80% water fly gourd extract to PC50-PC90 phospholipid is 1:0.5-1:

2. ​

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