Cosmetic composition with functions of moisturizing skin, whitening skin and improving wrinkles

Abstract

The invention provides a cosmetic composition with functions of moisturizing skin, whitening skin and improving wrinkles. The cosmetic composition of the present invention contains a frog and melon extract and a walnut extract, and can maintain skin moisture for a long period of time even after application is stopped, and provides an excellent moisturizing effect and an excellent whitening effect by reducing the melanin content of skin cells. The skin wrinkles are improved by increasing the biosynthesis rate of collagen.

Description

Technical Field

[0001] This invention provides cosmetic compositions, quasi-pharmaceutical compositions, etc., with skin moisturizing, whitening and wrinkle-improving functions. Background Technology

[0002] As human skin ages, it undergoes changes due to various internal and external factors. Internally, the secretion of hormones regulating metabolism decreases, the function and activity of immune cells decline, and the biosynthesis of essential immune proteins and biological components decreases. Externally, ozone layer depletion leads to increased ultraviolet radiation reaching the sun's surface, and with worsening environmental pollution, free radicals and harmful reactive oxygen species increase, resulting in dry skin, increased wrinkles, and decreased elasticity. Skin tone becomes dull, skin problems become more frequent, and spots, freckles, and age spots increase, causing various changes.

[0003] To mitigate or mitigate these changes, various cosmetics are being developed, and extensive research is currently underway using a wide range of natural products. Therefore, while there are many examples confirming the efficacy of a single natural product, there are fewer examples demonstrating enhanced efficacy through the appropriate combination of two or more natural products, thus requiring further research.

[0004] Existing technical documents

[0005] Patent documents

[0006] Patent Document 1: Korean Patent Registration Announcement 10-1884660 Summary of the Invention

[0007] The problem that the invention aims to solve

[0008] The inventors of this invention discovered during their research on cosmetics utilizing various natural products that combining frog melon extract and walnut extract to create cosmetics resulted in excellent moisturizing, whitening, and wrinkle-improving effects, thus completing this invention.

[0009] Therefore, the object of the present invention is to provide a cosmetic composition containing frog melon extract and walnut extract.

[0010] Another object of the present invention is to provide a quasi-pharmaceutical composition containing frog melon extract and walnut extract.

[0011] Another object of the present invention is to provide a method for preparing a cosmetic composition containing frog extract and walnut extract.

[0012] means for solving problems

[0013] To achieve the objectives of this invention, a cosmetic composition containing frog melon extract and walnut extract is provided.

[0014] In addition, the present invention provides a quasi-pharmaceutical composition containing frog melon extract and walnut extract.

[0015] In one embodiment of the present invention, the weight ratio of frog melon extract and walnut extract can be 1:1 to 1:2.

[0016] As an embodiment of the present invention, the weight ratio of frog melon extract and walnut extract can be 1:1.5.

[0017] As an embodiment of the present invention, the frog melon extract and walnut extract can be methanol extracts.

[0018] As an embodiment of the present invention, the cosmetic composition may be formulated as a hand care essence.

[0019] As an embodiment of the present invention, the cosmetic composition includes skin whitening, moisturizing, and wrinkle-improving effects.

[0020] As an embodiment of the present invention, the composition can be used for skin whitening, skin moisturizing, or skin wrinkle improvement.

[0021] In addition, the present invention provides a method for producing a cosmetic composition, comprising the following steps:

[0022] a) Chop the dried frog legs and walnuts;

[0023] b) Soak the chopped frog legs and walnuts in 70% methanol for 5 days.

[0024] c) Filtration yields extracts of frog and walnut; and

[0025] d) Mix the extract with cosmetic ingredients to prepare a cosmetic composition.

[0026] Invention Effects

[0027] The cosmetic composition of the present invention is characterized by containing frog melon extract and walnut extract. This cosmetic composition retains skin moisture for a long time, even after discontinuation of use, and provides excellent moisturizing and whitening effects by reducing the melanin content of skin cells. It is highly effective in increasing collagen biosynthesis and improving skin wrinkles. Detailed Implementation

[0028] While researching cosmetics using various natural products, the inventors combined frog melon extract and walnut extract in the manufacture of cosmetics, such as moisturizing and whitening products. The results showed excellent wrinkle-improving effects, thus completing this invention.

[0029] Therefore, the present invention provides a cosmetic composition containing frog melon extract and walnut extract.

[0030] In this invention, the frog melon (Cucumis koreana) is an ecotype species that evolved into the Asian melon during the dispersal of a wild species native to India. Also known as the Chenghuan melon, its main stem branches laterally and climbs onto other objects using tendrils. The stem is hairy, and the fruit is a cylindrical-elliptical berry that can be eaten.

[0031] In this invention, the walnut is a drupe obtained from a tree belonging to the genus *Sibirica*. In this invention, the portion used to obtain the extract refers to the kernel after the outer shell has been removed.

[0032] In addition to the above-described compositions, the cosmetic compositions according to the present invention may, as needed, contain various ingredients commonly used in cosmetic compositions, such as water-soluble ingredients, powder ingredients, oils, surfactants, and moisturizers, provided they meet the requirements. Viscosity modifiers, preservatives, antioxidants, fragrances, pigments, etc., may be combined without impairing the effects of the present invention.

[0033] The cosmetic compositions of the present invention include solutions, topical ointments, creams, foams, nourishing lotions, softening lotions, face masks, toners, emulsions, base makeup agents, fragrances, soaps, liquid cleansers, bath salts, sunscreens, sun oils, and suspensions, which can be prepared into formulations selected from emulsions, pastes, gels, emulsions, powders, soaps, surfactant-containing cleansers, oils, foundations, emulsion foundations, wax foundations, patches, and sprays.

[0034] The cosmetic composition of the present invention may also contain one or more cosmetically acceptable carriers that can be mixed with general skin cosmetics. Common ingredients include, for example, oils, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, colorants, and may be appropriately mixed with preservatives, fragrances, etc., but are not limited thereto.

[0035] The cosmetically acceptable carrier contained in the cosmetic compositions of the present invention varies depending on the formulation of the cosmetic composition.

[0036] When the formulation of the present invention is an ointment, paste, cream, or gel, the carrier component includes animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, and zinc oxides, but is not limited thereto. These components can be used alone or in combination of two or more.

[0037] When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc., can be used as carrier components. Especially in the case of a spray, chlorofluorocarbons can be added. These may include, but are not limited to, propellants such as low-carbon, propane / butane, or dimethyl ether. They can be used alone or in combination of two or more.

[0038] When the formulation of the present invention is a solution or emulsion, solvents, solubilizers, or emulsifiers can be used as carrier components, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, and benzyl benzoate. Propylene glycol, 1,3-butanediol oil, and especially cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil, and sesame oil, aliphatic glycerides, polyethylene glycol, or dehydrated sorbitol fatty acids can be used, but are not limited thereto. They can be used alone or in combination of two or more.

[0039] When the formulation of the present invention is a suspension, the carrier component includes water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitol ester, microcrystalline cellulose, and aluminum hydroxide, which may be bentonite, agar or tracant, but is not limited thereto. They may be used alone or in combination of two or more.

[0040] When the formulation of this invention is soap, the carrier components include alkali metal salts of fatty acids, fatty acid half-ester salts, fatty acid protein hydrolysates, hydroxyethyl sulfonates, lanolin derivatives, fatty alcohols, vegetable oils, glycerin, sugars, etc. These can be used, but are not limited to. They can be used alone or in combination of two or more.

[0041] In this invention, the weight ratio of frog melon extract to walnut extract can be from 1:1 to 1:2, preferably 1:1.5. The inventors believe that frog melon and walnut extract can moisturize the skin and have been proven to have whitening and wrinkle-improving effects. In particular, it has been shown that a weight ratio of frog melon extract to walnut extract of 1:1.5 is significantly superior and effective (Examples 1 to 3).

[0042] In this invention, the frog melon extract and walnut extract can be methanol extracts.

[0043] This includes results obtained by immersing the target substance in a solvent and then extracting it for a certain period of time at low, normal, or warm temperatures, resulting in a liquid component (extract) and the solid component obtained after removing the extract. The solvent is a concept defined as a liquid.

[0044] In this invention, the dosage form of the cosmetic composition can be a hand care essence.

[0045] In this invention, the cosmetic composition is used for skin whitening and can moisturize and improve wrinkles.

[0046] Skin moisturizing effect refers to the ability to retain skin moisture for an extended period of time in the air.

[0047] Skin whitening refers to enhancing the function of melanocytes in the skin, thereby preventing excessive melanin deposition or thinning existing melanin. Therefore, it can inhibit the formation of spots or freckles caused by excessive melanin deposition.

[0048] Wrinkle improvement refers to maintaining or strengthening the skin's wrinkles and elasticity. Collagen (collagen fibers in the dermis) and elastin (elastic fibers) are the main proteins that play this role and control skin elasticity. Therefore, they inhibit the activity of secreted elastase and / or collagenase. When used, they can improve wrinkles and also prevent skin aging.

[0049] The aforementioned prevention of skin aging refers to preventing or delaying time-related aging (intrinsic aging), which is the natural aging that occurs over time due to physiological changes within the body, and photoaging (photoinduced aging), which refers to aging that occurs in areas exposed to sunlight. To inhibit skin aging, it is essential not only to protect the skin from harmful external environmental influences but also to minimize wrinkle formation by activating skin cells and promoting the synthesis of biosynthetic proteins such as collagen and elastin. Specifically, in the structure of the skin, the dermis contains collagen (collagen fibers) and elastin (elastic fibers), forming a network structure. When this network structure is disrupted, elastin is broken down by the enzyme elastase, leading to sagging skin. Intrinsic skin aging, characterized by aging and wrinkles, is a consequence of skin aging. Therefore, skin aging can be inhibited by suppressing the breakdown of elastin, one of the main causes of skin aging.

[0050] Specifically, the skin aging described in this invention can be photoaging caused by UV-B ultraviolet radiation, but is not limited to this. Specifically, photoaging of skin cells may occur due to the wavelengths of light in sunlight ranging from 280 nm to 40 nm. In particular, irradiation with ultraviolet radiation such as UV-B in the wavelength range of 280 nm to 320 nm can damage the skin or fibers and may cause skin darkening. When exposed to UV-B, the accumulation of reactive oxygen species (e.g., H2O2) and free radicals is promoted within skin cells. Free radicals stimulate intracellular signaling systems, causing oxidative stress on biomolecules such as DNA, proteins, and lipids. This may cause damage to skin tissue. When oxidative stress in skin cells increases, keratinocytes in the epidermis or fibroblasts in the dermis are stimulated, producing genes such as MMPs (matrix metalloproteinases) and collagen-degrading enzymes through a series of intracellular signaling processes.

[0051] Increased hydration may lead to a decrease in collagen, the main component of skin, making up 90% of the dermis. Collagen protects the skin from external stimuli or forces by providing strength and tension. This can result in skin aging or wrinkles.

[0052] On the other hand, the present invention provides a pharmaceutical composition comprising frog melon extract and walnut extract.

[0053] According to conventional methods, the pharmaceutical compositions of the present invention may further comprise pharmaceutically acceptable carriers, excipients, or diluents. Pharmaceutically acceptable carriers are well known in the art, depending on the route of administration or dosage form, and specific examples can be found in pharmacopoeias of various countries, including the Korean Pharmacopoeia. Carriers, excipients, and diluents that may be included in the compositions of the present invention include lactose, dextran, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum arabic, alginate, gelatin, calcium phosphate, and calcium silicate. Examples include, but are not limited to, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylparaben, propylparaben, talc, magnesium stearate, and mineral oil. Furthermore, carriers, excipients, and diluents that may be included in the compositions of the present invention may be non-natural carriers, but are not limited thereto.

[0054] The pharmaceutical compositions of the present invention can be formulated by conventional methods into oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, topical preparations, suppositories, or sterile injections. Specifically, it can be prepared using common diluents or excipients such as fillers, expanders, binders, wetting agents, disintegrants, and surfactants. Solid dosage forms for oral administration include tablets, pills, powders, granules, capsules, etc. These solid dosage forms contain the aforementioned compounds and at least one excipient, such as starch, calcium carbonate, sucrose, lactose, gelatin, or ETC. In addition to simple excipients, lubricants such as magnesium stearate and talc can also be used. Liquid dosage forms for oral administration include suspensions, oral solutions, emulsions, and syrups, and may contain various excipients such as wetting agents, sweeteners, flavorings, and preservatives, in addition to common simple diluents such as water and liquid paraffin. Preparations intended for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized formulations, and suppositories. Non-aqueous solvents and suspensions include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As a base for suppositories, Withepsol, Marc Rogol, Tween 61, cocoa, laurel, glycerin gelatin, etc., can be used. For specific dosage forms of pharmaceutical compositions known in the art, please refer to, for example, Remington's Pharmaceutical Sciences (19th edition, 1995). The above documents are considered part of this specification.

[0055] On the other hand, the present invention provides a quasi-pharmaceutical composition containing frog melon extract and walnut extract.

[0056] In this invention, a quasi-medicine refers to a product that has a milder effect than a pharmaceutical product in the context of diagnosing, treating, improving, alleviating, treating, or preventing human or animal diseases, for example, according to the provisions of the Act Governing the Affairs of the Republic of Korea. Quasi-medicines do not include products used for pharmaceutical purposes, but include products used to treat or prevent human and animal diseases, and products that have little or no direct effect on the human body.

[0057] The quasi-pharmaceutical compositions of the present invention can be manufactured in the form of ointments, lotions, sprays, patches, creams, powders, suspensions, gels or filter fillers, but are not limited thereto.

[0058] When the composition according to the invention is used as a quasi-pharmaceutical additive, the composition can be added directly or used in conjunction with other quasi-pharmaceutical or quasi-pharmaceutical ingredients, and can be used appropriately according to conventional methods. The amount of active ingredient mixed can be appropriately determined according to the intended use.

[0059] On the other hand, the present invention provides a method for producing a cosmetic composition, comprising the following steps:

[0060] a) Chop the dried frog legs and walnuts;

[0061] b) Soak the chopped frog legs and walnuts in 70% methanol for 5 days.

[0062] c) Filtration yields extracts of frog and walnut; and

[0063] d) Mix the extract with cosmetic ingredients to prepare a cosmetic composition.

[0064] Chopping and cold soaking can be performed using methods commonly used in the art. Walnuts should only be chopped after the outer skin has been removed. Filtration can be performed using ordinary filter paper, but is not limited to this. Cosmetic ingredients, such as glyceryl stearate, which are commonly used in cosmetic manufacturing, can be used as commercially available materials.

[0065] Preferred embodiments are given below to aid in understanding the present invention. However, the following embodiments are provided merely to make the present invention easier to understand, and the content of the present invention is not limited to the following embodiments.

[0066] Preparation Example 1: Preparation of Frog Melon and Walnut Extract

[0067] Frog gourd and peeled walnuts were dried at -10℃ for 5 days, then chopped and 100g of each dry weight were placed in separate flasks and immersed in 500g of 70% methanol for cold extraction for 5 days. The extracts were filtered through a 0.3μm filter to obtain frog gourd and walnut extracts. Frog gourd and walnut extracts were prepared by mixing the extracts at weight ratios of 1:0.5, 1:1, 1:1.5, 1:2, and 1:2.5, as shown in Table 1.

[0068] Table 1

[0069]

[0070] Preparation Example 2: Preparation of Hand Care Essence Composition

[0071] The above-mentioned raw materials are mixed as listed in Table 2 below, and the hand care essence composition is prepared by conventional methods.

[0072] Table 2

[0073]

[0074]

[0075] Experimental Example 1: Confirmation of the moisturizing effect of the extract of the present invention

[0076] To confirm the moisturizing effect of the extract of the present invention, hand serum samples 1 to 5 of Preparation Example 1 were prepared by the method of Preparation Example 2 using samples 1 to 5 of Preparation Example 1. Additionally, as a comparative example, a hand serum without the extract of the present invention was also prepared according to the method of Preparation Example 2.

[0077] Sixty women over 40 years old with dry skin were selected and divided into 6 groups of 10 each. Hand serum samples 1-5 and a control group were tested. The hand serum was applied to the face twice daily for 4 weeks. Skin moisture was measured before application, at 1 week, 2 weeks, 4 weeks after application, and 1 week after discontinuation (a total of 5 weeks) under constant temperature and humidity conditions (24℃, 40% relative humidity). Measurements were performed using a Corneometer CM825 (C+K ElectronicCo., Germany). The results are shown in Table 3 below. The results in Table 3 are based on the skin moisture meter values ​​measured before the start of the test, and are expressed as a percentage increase in the measured values ​​after a certain period of treatment.

[0078] Table 3

[0079]

[0080] As shown in Table 3, before four weeks of continuous use, the control group exhibited an increase in skin hydration of approximately 30% or more; however, a sharp decline in skin moisture was observed after discontinuation of use. On the other hand, when the product contained extracts of water chestnut and walnut, a higher rate of increase in hydration was observed even after discontinuation of application. Particularly when the ratio of water chestnut and walnut extracts was 1:1.5, the rate of increase in hydration exceeded 30%, demonstrating a significant effect. This indicates excellent moisturizing efficacy.

[0081] Experimental Example 2: Confirmation of the whitening effect of the extract of the present invention

[0082] The skin-whitening effect of the extract of this invention was evaluated, along with its melanin production inhibition effect. Human melanoma cell line MNT1 cells containing 20% ​​fetal bovine serum in at least a basic culture medium (MEM) were incubated at 37°C and 5% CO2. Cell counts were performed at 1 x 10⁶ cells per plate. 5Prepare 6 dishes each night, the more the better. After the period of time, the mixed extracts of frog and walnut in samples 1 to 5 in Table 1 were obtained. Each was added to 50mM with the same badge I changed. Also for comparison for this purpose, the above mixed extracts did not contain badges used with cultured cells. Badges were added to the cells on day 2 after each change of the chalk in the dish for 5 days. When the cells were fully grown, they were treated with 300μl of buffer (1% NP-40, 50mM Tris-HCl; pH 7.5, 150mM NaCl). Next, the cells were collected in microtubes using a cell scraper, centrifuged at 15,000rpm for 10 minutes, and the absorbance of the dried precipitate at 450nm was measured, and the results are shown in Table 4.

[0083] Table 4

[0084] Ratio of frog melon and walnut extracts Absorbance (450nm) 1:0.5 0.26 1:1 0.23 1:1.5 0.17 1:2 0.18 1:2.5 0.23 Extracts (comparative group) 0.3 8

[0085] As shown in Table 4 above, the melanin content in the cells of the control group not treated with frog melon and walnut extract did not decrease significantly, while the absorbance of the control group treated with frog melon and walnut extract showed a large difference, indicating that the melanin content in the cells did not decrease significantly, thus confirming that the melanin content was significantly reduced.

[0086] Experimental Example 3: Confirming the wrinkle-improving effect of the extract of the present invention

[0087] To verify the wrinkle-improving effect of the extract of the present invention, the collagen production effect was measured. Skin wrinkles are caused by insufficient collagen production. Collagen is a major protein in the dermis, playing a crucial role in maintaining skin structure and elasticity. Its production decreases with age, leading to increased dermal collapse and inducing wrinkle formation, as is known. Dermal fibroblasts are responsible for the synthesis of collagen, the main protein in skin cells. Therefore, the degree to which fibroblasts increase collagen production was assessed by measuring the extract's ability to improve collagen synthesis and its impact on wrinkle improvement.

[0088] The extent of collagen synthesis was tested at the cellular level using fibroblast culture. The fibroblasts were derived from the human HDFn cell line (C-004-5C: Cascade Biologics). The fibroblasts were cultured at a concentration of 1 × 10⁶ cells / year. 6Cells / well were seeded into 48-well plates and cultured in DMEM medium containing 10% serum at 37°C and 5% CO2 for 24 hours. Then, samples of *Gnaphalium affine* and *Hedychium coronarium* extracts from Table 1 were exchanged for serum-free DMEM medium at 1 to 550 mM and cultured for 24 hours. Additionally, for comparison, a control group identical to the above but without *Gnaphalium affine* and *Hedychium coronarium* extracts was prepared. After culture, the supernatant was collected and centrifuged at 14,000 rpm for 4 minutes. Only the supernatant was collected, and the amount of type I procollagen C-peptide (PIP) was calculated using a kit (Takara) to determine the amount of newly synthesized collagen. Collagen was measured. The amount of PIP was converted to ng / ml, and the collagen biosynthesis rate was calculated according to the following formula and listed in Table 5.

[0089] Collagen production rate (%) = (Collagen amount in experimental group / Collagen amount in control group) × 100

[0090] Table 5

[0091] Ratio of frog melon and walnut extracts PIP (based on 100% control group) 1:0.5 11 5％ 1:1 121％ 1:1.5 12 8％ 1:2 122％ 1:2.5 1 19％ Extracts (comparative group) 100％

[0092] Table 5 shows that when treated with extracts of *Gnaphalium affine* and walnut, the PIP synthesis effect was superior to that of the control group, thus exhibiting a higher skin wrinkle improvement effect compared to the control group, especially with the extracts of *Gnaphalium affine* and walnut. A significantly superior skin wrinkle improvement effect was observed when the walnut extract content ratio was 1:1.5.

Claims

1. A cosmetic composition, wherein, The cosmetic composition contains frog extract and walnut extract.

2. The cosmetic composition according to claim 1, wherein, The weight ratio of frog melon extract to walnut extract is 1:1 to 1:

2.

3. The cosmetic composition according to claim 1, wherein, The weight ratio of frog melon extract to walnut extract is 1:1.

5.

4. The cosmetic composition according to claim 1, wherein, Frog melon extract and walnut extract are methanol extracts.

5. The cosmetic composition according to claim 1, wherein, The cosmetic composition is formulated as a hand care essence.

6. The cosmetic composition according to claim 1, wherein, This cosmetic composition includes skin whitening, moisturizing, and wrinkle-reducing effects.

7. A quasi-pharmaceutical composition, wherein, The quasi-pharmaceutical composition contains frog extract and walnut extract.

8. The quasi-pharmaceutical composition according to claim 7, wherein, The weight ratio of frog extract to walnut extract is 1:1 to 1:

2.

9. The quasi-pharmaceutical composition according to claim 7, wherein, The weight ratio of frog melon extract to walnut extract is 1:1.

5.

10. The quasi-pharmaceutical composition according to claim 7, wherein the quasi-pharmaceutical composition comprises skin whitening, moisturizing, and wrinkle-improving effects.

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