Skin-refreshing composition containing acid enzyme peptide, product and application of skin-refreshing composition

Abstract

The invention provides a skin care composition containing acid enzyme peptide. The skin care composition comprises lactobionic acid, hexanoyl dipeptide-3-n-leucine and papain, the mass ratio of the lactobionic acid to the hexanoyl dipeptide-3-n-leucine to the papain is (0.1 to 15) to (0.5 to 15) to (0.05 to 5). The invention further provides a product containing the composition and application of the product. According to the invention, lactobionic acid, papain and hexanoyl dipeptide-3-n-leucine are compounded for use, when the composition is used for biological skin renewal, namely exfoliating, the three components interact with each other and have a synergistic effect, the content of eluted keratin can be remarkably increased, and the composition has a good exfoliating effect. In addition, the composition provided by the invention is relatively low in irritation.

Description

Technical Field

[0001] This application relates to the field of daily chemical products technology, and in particular to a skin-rejuvenating composition, product and application containing acid enzyme peptides. Background Technology

[0002] Human skin consists of the epidermis and dermis. The outermost layer of the skin is the stratum corneum, which protects against external stimuli. However, the shedding of the stratum corneum leads to the accumulation of dead skin cells, forming a thick, rough stratum corneum. This thick stratum corneum makes the skin appear rough and with irregular skin lines, making it look dull and wrinkled. It also affects the penetration and absorption of cosmetics, hindering their effectiveness.

[0003] Currently, an increasing number of exfoliating products are made with bio-exfoliating ingredients, which are generally enzymes that dissolve and remove dead skin cells. These ingredients are relatively gentle and cause less skin irritation. However, the exfoliating effect of enzyme-based exfoliating ingredients still needs improvement. Summary of the Invention

[0004] In view of this, this application provides a skin-renewing composition, product and application containing acid enzyme peptides. The skin-renewing composition provided by this application has a synergistic effect in increasing the content of exfoliated keratin proteins and has good biological skin-renewing and exfoliating effects.

[0005] This application provides a skin-renewing composition containing acid-enzyme peptides, including lactobionic acid, hexanoyl dipeptide-3 leucine, and papain;

[0006] The mass ratio of lactobionic acid, hexanoyl dipeptide-3-leucine, and papain is 0.1~15: 0.5~15:0.05~5.

[0007] In some specific implementations, the mass ratio of lactobionic acid, hexanoyl dipeptide-3 leucine, and papain is 0.3~10:0.8~12:0.08~3.

[0008] In some specific implementations, the mass ratio of lactobionic acid, hexanoyl dipeptide-3 leucine, and papain is 0.5~6:1~10:0.1~2.

[0009] In some specific implementations, the mass ratio of lactobionic acid, hexanoyl dipeptide-3 leucine, and papain is 6:5:0.1, 4:3:1, 2:1:2, or 0.5:10:1.5.

[0010] This application also provides the use of the skin-renewing composition described above in the preparation of skin-renewing and / or exfoliating products.

[0011] In some specific implementations, the exfoliation process involves increasing the amount of keratin proteins removed.

[0012] This application also provides an exfoliating product, comprising the skin-renewing composition described in the above-mentioned technical solution.

[0013] In some specific implementations, the content of the skin-renewing composition is 1wt% to 10wt%.

[0014] In some specific implementations, the product is a cosmetic.

[0015] This application also provides the application of the skin-renewing products described in the above technical solutions in the preparation of skin-renewing and / or exfoliating products.

[0016] This application provides an exfoliating composition containing an acid-enzyme peptide, comprising lactobionic acid, hexanoyl dipeptide-3-leucine, and papain; the mass ratio of lactobionic acid, hexanoyl dipeptide-3-leucine, and papain is 0.1~15:0.5~15:0.05~5. This application uses a combination of lactobionic acid, papain, and hexanoyl dipeptide-3-leucine for bio-resurfacing, i.e., exfoliation. The three components interact synergistically, significantly increasing the content of exfoliated keratin proteins and resulting in excellent exfoliation effects. Furthermore, the composition provided in this application has low irritation. Attached Figure Description

[0017] Figure 1 The results show the elution keratin content test results of different groups in Test Example 1 of this application. Detailed Implementation

[0018] It should be understood that the expression “one or more of…” individually includes each of the objects described after the expression, as well as various different combinations of two or more of the described objects, unless otherwise understood from the context and usage. The expression “and / or” combined with three or more described objects should be understood to have the same meaning, unless otherwise understood from the context.

[0019] The terms “including,” “having,” or “containing,” including the use of their grammatical synonyms, should generally be understood as open-ended and non-restrictive, for example, not excluding other unstated elements or steps, unless otherwise specifically stated or understood from the context.

[0020] It should be understood that the order of steps or the sequence of actions is not important as long as this application remains operational. Furthermore, two or more steps or actions may be performed simultaneously.

[0021] The use of any and all instances or exemplary language such as “e.g.” or “including” herein is merely intended to better illustrate the application and does not constitute a limitation on the scope of the application unless the claims are made. No language in this specification should be construed as indicating that any unclaimed element is essential to the practice of this application.

[0022] Furthermore, the numerical ranges and parameters used to define this application are approximate values, and the relevant values ​​in the specific embodiments have been presented as precisely as possible. However, any value inevitably contains standard deviations due to individual test methods. Therefore, unless otherwise explicitly stated, it should be understood that all ranges, quantities, values, and percentages used in this disclosure are modified with the word "approximately." Here, "approximately" generally means that the actual value is within plus or minus 10%, 5%, 1%, or 0.5% of a specific value or range.

[0023] This application provides a skin-renewing composition containing acid-enzyme peptides, including lactobionic acid, hexanoyl dipeptide-3 leucine, and papain;

[0024] The mass ratio of lactobionic acid, hexanoyl dipeptide-3-leucine, and papain is 0.1~15: 0.5~15:0.05~5.

[0025] The skin-renewing composition provided in this application includes lactobionic acid, which is a fruit acid that can slowly exfoliate old keratinocytes by loosening the connections between keratinocytes, improving roughness and dullness, reducing clogged pores, and is relatively gentle and will not cause significant peeling.

[0026] The skin-renewing composition provided in this application includes hexanoyl dipeptide-3 leucine, which is also known as fruit acid peptide. It is a tripeptide compound composed of a dipeptide backbone, leucine, and hexanoyl group. It can activate the skin's own renewal process, target the formation of keratinized desmosomes between dead skin cells, loosen old dead skin cells, and achieve gentle exfoliation and accelerate cell regeneration.

[0027] The skin-renewing composition provided in this application includes papain, which can break down keratin proteins on the skin surface and metabolize dead skin cells. It has a gentle effect and low irritation.

[0028] In this application, the mass ratio of lactobionic acid, hexanoyl dipeptide-3-leucine, and papain is 0.1~15:0.5~15:0.05~5, preferably 0.3~10:0.8~12:0.08~3, more preferably 0.5~6:1~10:0.1~2, and most preferably 6:5:0.1, 4:3:1, 2:1:2, or 0.5:10:1.5.

[0029] This application also provides a skin-renewing product, comprising the exfoliating composition described in the above-described technical solution.

[0030] In some embodiments of the present invention, the above-mentioned products include, but are not limited to, personal care products, pharmaceuticals, etc. Those skilled in the art will understand that, in addition to the compositions described in the above technical solutions, other excipients are also included, such as pharmaceutical excipients or excipients for personal care products.

[0031] In some embodiments of the present invention, the personal care products include: basic care products and / or makeup products.

[0032] In some embodiments of the present invention, the cosmetic products include, but are not limited to:

[0033] (1) Base makeup:

[0034] Foundation / cream: Used to even out skin tone and cover blemishes;

[0035] BB cream / CC cream: A lightweight base makeup product that combines skincare and makeup application;

[0036] Concealer / pen: Used to cover up specific areas such as pimples and dark circles;

[0037] Loose powder / setting powder: Sets makeup and reduces facial shine.

[0038] (2) Eye makeup:

[0039] Eyeshadow: Adds color and dimension to the eyes;

[0040] Eyeliner pencil / liquid / gel: Used to draw lines on the eyes, making them look more vibrant;

[0041] Mascara: Lengthens and thickens eyelashes, enhancing the depth of the eyes;

[0042] Eyebrow pencil / powder / gel: Fill in gaps in eyebrows and create the ideal eyebrow shape.

[0043] (3) Cheek makeup:

[0044] Blush: Adds natural color to the cheeks and improves complexion;

[0045] Contouring powder / stick: Use shading techniques to make facial contours more three-dimensional;

[0046] (4) Lip makeup:

[0047] Lipstick / lip gloss / lip tint: to change or emphasize the color of the lips;

[0048] Lip liner: Defines the clear outline of the lips and prevents lipstick from bleeding.

[0049] (5) Multifunctional cosmetics:

[0050] Highlighter sticks / liquids / powders: Highlight high points on the face (such as the bridge of the nose and cheekbones) to create a luminous effect.

[0051] In addition, there are products designed specifically for special occasions, such as waterproof and sweatproof eyeliners and long-lasting, non-fading lipsticks.

[0052] In some embodiments of the present invention, the basic care products include, but are not limited to:

[0053] Facial cleanser: Gently removes dirt, oil, and makeup residue from the face;

[0054] Cleansing oil / cleansing water / cleansing balm: specially designed to thoroughly remove makeup, especially waterproof cosmetics;

[0055] Toner / lotion: Used after cleansing, it can further cleanse the skin's surface of residue, while replenishing the skin's moisture, restoring the skin's pH balance, and laying a good foundation for the absorption of subsequent skin care products;

[0056] Serum: Contains a high concentration of active ingredients, providing deep nourishment and repair for specific skin problems (such as anti-aging, moisturizing, whitening, etc.);

[0057] Eye cream: Specially designed for the sensitive area around the eyes, it has the effects of reducing fine lines and dark circles and firming the skin around the eyes. The texture is usually light and easily absorbed.

[0058] Day / night lotion or cream: It has functions such as protection, repair and nourishment, and promotes cell regeneration. It can provide the skin with the necessary moisture and lock in the replenished moisture.

[0059] Sunscreen: Used for ultraviolet protection and prevention of photoaging, etc.

[0060] Face masks: provide extra nourishment and care for the skin, such as hydration, pore cleansing, or brightening of skin tone.

[0061] In some specific implementations, the personal care product includes 0.1wt% to 10wt% of the composition described in the above technical solution, preferably 0.5wt% to 9.5wt% of the composition described in the above technical solution, and more preferably 1wt% to 9wt% of the composition described in the above technical solution.

[0062] The composition and products containing the composition provided in this application can be used for exfoliation or bio-resurfacing. The combination of lactobionic acid, hexanoyl dipeptide-3 leucine, and papain, with their interaction, has a synergistic effect in increasing the content of exfoliated keratin, resulting in excellent exfoliation. Furthermore, the composition provided in this application has low irritation.

[0063] The present application is further illustrated below with reference to embodiments. The scope of protection of the present application is not limited to the following embodiments.

[0064] In the following examples and comparative examples, raw material A is lactobionic acid; raw material B is a solution containing hexanoyl dipeptide-3 leucine, wherein the solvent is glycerol and water, and the content of hexanoyl dipeptide-3 leucine active ingredient is >100ppm; raw material C is papain with enzyme activity ≥600,000 U / g.

[0065] Examples 1-4, Comparative Examples 1-1-4-2

[0066] Prepare the composition according to the formulation shown in Table 1:

[0067] Table 1 Formulations of Examples and Comparative Examples

[0068]

[0069] The preparation method is as follows:

[0070] Components A, B, and C were mixed in the proportions shown in Table 1 to obtain the composition.

[0071] Experimental Example 1

[0072] 1.1 Test Method

[0073] Using an in vitro porcine skin exfoliation efficacy evaluation model, the exfoliation effect of different groups was assessed by quantitative results of BCA protein.

[0074] 1.2 Test Materials

[0075] 1.2.1 Main Reagents and Materials

[0076] The ingredients include Bama miniature pig skin, sodium hydroxide, and hydrochloric acid.

[0077] 1.2.2 Main Instruments and Consumables

[0078] Analytical balance (Mettler Toledo ML204 / 02), microplate reader (Thermo MULTISKAN GO), 96-well culture plate, BCA protein quantification kit (Shanghai Beyotime), and transdermal analyzer.

[0079] 1.3 Test Methods

[0080] 1.3.1 Experimental Procedure

[0081] 1) After thawing and cleaning the pigskin, fix the skin between the supply chamber and the receiving chamber of the Franz cell diffusion cell, with the stratum corneum of the skin facing the supply chamber and the dermis facing the receiving chamber. Add receiving fluid to the receiving chamber, tighten and fix the pigskin, add receiving fluid to remove air, and make the dermis of the skin in close contact with the receiving fluid.

[0082] 2) Add the sample to the skin surface in the supply chamber. Add 100 μL of the sample at the concentration shown in Table 2 to the pig skin surface (PBS buffer was added to the control group surface as a blank control); spread the sample evenly from the center of the skin outwards radially, with an exposed sample area of ​​3.14 cm². Each sample was tested in triplicate; maintain a constant temperature water bath at (32±1) ℃, ensuring that there are no air bubbles in the water bath jacket.

[0083] 3) After incubation for 30 minutes, put on a finger cot and rub the sample application area. After rubbing for two minutes, add 0.5 mL of cleaning solution (0.1% Triton X) to the supply chamber and blow to clean the sloughed keratinocytes off the skin surface.

[0084] 4) Transfer the cleaning solution to a 1.5 mL centrifuge tube. Place the collected cleaning solution in a high-speed centrifuge, set the speed to 15000 rpm, and centrifuge for 10 min. The keratinocytes in the cleaning solution will precipitate. Discard the supernatant and resuspend the precipitate in 0.5 mL of deionized water.

[0085] 5) Add 25 μL of sodium hydroxide (12M) solution to the resuspended liquid, lyse the keratinocytes by boiling water bath for 30 min, add 25 μL of hydrochloric acid to stop the lysis reaction, centrifuge for 10 min, and take the supernatant for protein quantification.

[0086] 6) Test the protein content according to the kit requirements.

[0087] 1.3.2 Indicator Testing

[0088] BCA was used to quantify the content of exfoliated keratin in each group, and the rate of change in the content of exfoliated keratin in different groups relative to the control group was calculated using the following formula:

[0089]

[0090] Wherein, E (test group) is the content of eluted keratin obtained in each example group or comparative example group, and E (blank group) is the content of eluted keratin obtained in the blank group;

[0091] According to the King's method, the synergy coefficient is calculated using the following formula:

[0092]

[0093] Among them, EAB E represents the exfoliation promotion rate of the combined two drugs (Example X of this application). A E represents the exfoliation promotion rate of single agent A (comparative example X-1 of this application). B The exfoliation promotion rate of single agent B (comparative example X-2 of this application);

[0094] Q < 0.85 indicates that the two drugs have an antagonistic effect when used together; Q > 1.15 indicates that the two drugs have a synergistic effect when used together; 0.85 ≤ Q ≤ 1.15 indicates that the two drugs have an additive effect when used together.

[0095] 1.4 Test Results

[0096] The test results are shown in Table 2 and Figure 1 Table 2 shows the test results of the eluted keratin content in different groups in Experiment Example 1 of this application. Figure 1 The results show the elution keratin content test results of different groups in Test Example 1 of this application.

[0097] Table 2. Protein content test results of different groups in Experiment Example 1 of this application.

[0098]

[0099] Figure 1 The results in Table 2 show that the content of keratinocyte protein eluted in the blank group was 49±18 μg / mL. The content of keratinocyte protein eluted in each treatment group of the examples was significantly higher than that in the blank group, and the effects of Examples 1 to 4 were better than those of the comparative examples, indicating that the composition can effectively promote the shedding of keratinocytes.

[0100] Based on the data in Table 2, Table 3 further calculates the exfoliation change rate (relative to the blank group) and synergy coefficient for each group. The results are shown in Table 3, which shows the exfoliation change rate for each group.

[0101] Table 3. Exfoliation Change Rates for Each Group

[0102]

[0103] The results showed that the synergistic coefficient of all compositions was greater than 1.15, confirming their synergistic effect within the experimental formulation range.

[0104] Experimental Example 2

[0105] The composition shown in Example 1 was prepared into a solution with the following concentrations: 6% raw material A, 5% raw material B and 0.1% raw material C. A human skin patch test was performed in accordance with Chapter 7, Section 2, Human Skin Patch Test, Section 6.1, Occlusive Skin Patch Test of the Cosmetic Safety Technical Specifications (2015 Edition).

[0106] 2.1 Materials and Methods

[0107] Test substance: Composition comprising 6 wt% lactobionic acid, 5 wt% hexanoyl dipeptide-3 leucine solution and 0.1 wt% papain, with the remainder being water.

[0108] Negative control: Blank filter paper control.

[0109] Participants: A total of 30 participants, including 6 males and 24 females, aged 21 to 41 years, with a mean age of 28.93 ± 6.88 years, meeting the criteria for voluntary inclusion.

[0110] 2.2 Spot Test Method

[0111] Using qualified patch testing equipment, approximately 0.020 mL of the test substance was placed in the patch testing device using the closed patch test method. The device was then applied to the flexor side of the subject's forearm with hypoallergenic adhesive tape. The test substance was removed after 24 hours. Skin reactions were observed at 0.5, 24, and 48 hours after removal. The results were recorded according to the skin reaction grading standards in the 2015 edition of the "Cosmetic Safety Technical Specifications" (as shown in Table 4).

[0112] Table 4. Grading Criteria for Skin Reaction in Occlusive Patch Tests

[0113]

[0114] 2.3 Test Results

[0115] The test results are shown in Table 5.

[0116] Table 5 Results of human skin patch test

[0117]

[0118] As shown in Table 5, the results of the human skin patch test showed that 0 out of 30 people experienced adverse skin reactions, indicating that the composition provided in this application has low irritation.

[0119] The above description is merely a preferred embodiment of this application, but the scope of protection of this application is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in this application, based on the technical solution and inventive concept of this application, should be included within the scope of protection of this application.

Claims

1. A skin-renewing composition containing an acid-enzyme peptide, comprising lactobionic acid, hexanoyl dipeptide-3 leucine, and papain; The mass ratio of lactobionic acid, hexanoyl dipeptide-3-leucine, and papain is 0.1~15: 0.5~15:0.05~5.

2. The skin-renewing composition according to claim 1, characterized in that, The mass ratio of lactobionic acid, hexanoyl dipeptide-3-leucine, and papain is 0.3~10:0.8~12:0.08~3.

3. The skin-renewing composition according to claim 2, characterized in that, The mass ratio of lactobionic acid, hexanoyl dipeptide-3-leucine, and papain is 0.5~6:1~10:0.1~2.

4. The skin-renewing composition according to claim 3, characterized in that, The mass ratio of lactobionic acid, hexanoyl dipeptide-3 leucine, and papain is 6:5:0.1, 4:3:1, 2:1:2, or 0.5:10:1.

5.

5. The use of the skin-renewing composition according to any one of claims 1 to 4 in the preparation of skin-renewing and / or exfoliating products.

6. The application according to claim 5, characterized in that, The exfoliation process aims to increase the amount of keratin proteins removed.

7. A skin-renewing product comprising the skin-renewing composition containing acid-containing peptides as described in any one of claims 1 to 4.

8. The skin-renewing product according to claim 7, characterized in that, The content of the skin-renewing composition is 0.1wt%~10wt%.

9. The skin-renewing product according to claim 8, characterized in that, It is a personal care product.

10. The use of the skin-renewing product according to any one of claims 7 to 9 in the preparation of skin-renewing and / or exfoliating products.

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