Sea cucumber source high-absorptivity calcium protein and preparation method thereof

Abstract

The invention belongs to the field of calcium protein, and discloses sea cucumber source high-absorbability calcium protein and a preparation method thereof.The sea cucumber source high-absorbability calcium protein is prepared from sea cucumbers, and viscera of the sea cucumbers are not removed when the calcium protein is prepared; 1 part by weight of calcium protein is prepared from 50 parts by weight of sea cucumbers; the preparation method comprises the following steps: S1, raw material pretreatment; s2, primary processing; s3, performing low-temperature freeze-drying; s4, crushing and sterilizing; s5, removing fishy smell and granulating; s6, filling and packaging capsules; according to the technical scheme, 100% pure natural sea cucumbers are used as the unique raw material to prepare the calcium protein, no chemical additives, preservatives, sweeteners and the like are used, the method is green and healthy, and the practicability and safety are improved; meanwhile, the applicability of the calcium protein is greatly improved, the calcium supplement product is suitable for different people to use, the problem that a conventional calcium supplement product cannot be absorbed is solved, the price is reasonable, the preparation process is simple and convenient, and industrial production and popularization are easy.

Description

Technical Field

[0001] This invention relates to the field of calcium proteins, and more particularly to a highly absorbable calcium protein derived from sea cucumber and its preparation method. Background Technology

[0002] Calcium is an essential macro-element for the human body, participating in various physiological processes such as bone formation, nerve conduction, and muscle contraction. Currently, calcium deficiency is widespread in my country, affecting as many as 900 million people, with infants, pregnant women, and the elderly being particularly vulnerable. There are approximately 90 million osteoporosis patients in my country, with an overall prevalence of 12.4%, reaching as high as 50% in the elderly. With the fast pace of modern life, high work pressure, and lack of exercise, people are prone to weakness, fatigue, and calcium loss. Therefore, calcium supplementation in daily life is essential. Currently used calcium supplements mainly include inorganic calcium (such as calcium carbonate) and organic calcium (such as calcium lactate and calcium citrate). Inorganic calcium has a high calcium content but poor solubility and causes significant gastrointestinal irritation, while organic calcium has better solubility but a lower calcium content. Both lack bioactive components that promote calcium absorption, resulting in a general absorption rate of less than 30% for the human body. In addition, some products add preservatives, sweeteners, and other chemicals to improve taste or extend shelf life. Long-term consumption may have potential health effects. Overall, these products are relatively expensive and have complex preparation methods, failing to meet the actual needs of most people. Summary of the Invention

[0003] The present invention aims to provide a sea cucumber-derived highly absorbable calcium protein and its preparation method, so as to solve the problems mentioned in the background art.

[0004] To achieve the above objectives, the present invention provides the following technical solution: A highly absorbable calcium protein derived from sea cucumber, wherein the calcium protein is prepared by processing sea cucumber without removing its internal organs, and the sea cucumber is selected from wild spiny sea cucumbers that have grown naturally for 3-5 years, with a protein content ≥60%, a calcium content ≥20mg / g, and a sea cucumber polysaccharide content ≥5%.

[0005] Preferably, 50 parts by weight of sea cucumber are used to prepare 1 part by weight of calcium protein.

[0006] A method for preparing a highly absorbable calcium protein derived from sea cucumber includes the following steps: S1. Raw material pretreatment: Select sea cucumber raw materials, remove impurities such as mud and shells attached to the sea cucumbers, rinse with purified water 3-5 times, and then soak in purified water for 48-72 hours. After soaking, rinse the sea cucumbers again with purified water until the water is clear, drain the water and set aside. S2. Preliminary processing: Mix the pretreated sea cucumber with purified water at a mass ratio of 4:6. After mixing, put the mixture into a high-speed pulper and pulp for 20-30 minutes and stir evenly to obtain sea cucumber raw material pulp. Put the sea cucumber raw material pulp into a stainless steel tank for later use. S3. Low-temperature freeze drying: Pour the sea cucumber raw material slurry obtained in step S2 into a freeze drying tray, control the thickness of the sea cucumber raw material slurry to be 2-4cm, put the freeze drying tray into a freeze dryer, and freeze dry at low temperature to obtain freeze-dried sea cucumber protein blocks. S4. Crushing and sterilization: The freeze-dried sea cucumber protein block obtained in step S3 is put into a vibrating ultra-micro pulverizer to crush the freeze-dried sea cucumber protein block into sea cucumber protein powder. The sea cucumber protein powder is sterilized by low-temperature plasma sterilization. The sterilized sea cucumber protein powder is placed in a sterile environment and cooled to room temperature to obtain storage raw material. S5. Deodorization and granulation: Take the storage raw material obtained in step S4, add a natural deodorizing agent to the storage raw material. The natural deodorizing agent is a compound of ginger extract and tangerine peel extract. The amount of natural deodorizing agent added is 1%-3% of the mass of the storage raw material. Put the storage raw material with added natural deodorizing agent into a three-dimensional mixer and mix for 15-20 minutes at a mixing speed of 20-30 r / min to obtain odorless sea cucumber protein powder. S6. Capsule filling and packaging: The odorless sea cucumber protein powder obtained in step S5 is put into a fully automatic capsule filling machine. Gastric-soluble gelatin capsules or plant-based capsules are used for filling, and the filling amount of each capsule is controlled to be 0.2-0.3g. After filling, the capsules are polished, and unqualified capsules are removed. Then, they are bottled according to specifications, and food-grade desiccant is added to each bottle before heat sealing. Finally, labels are affixed, and small, medium, and large-sized packaging boxes are used for packaging. The packaging boxes are then placed in a warehouse that meets the pharmaceutical GMP standards for storage, thus completing the preparation of calciprotein.

[0007] Preferably, in step S1, the temperature of the purified water used to soak the sea cucumber is controlled at 4°C.

[0008] Preferably, in step S1, the purified water is changed every 12 hours during the soaking of sea cucumbers.

[0009] Preferably, in step S3, after the freeze-drying tray is placed in the freeze dryer, it is first pre-frozen at a temperature of -40°C to -30°C for 2-3 hours, then sublimated and dried at a vacuum of 10-30 Pa and a shelf temperature of -20°C to -10°C for 48-72 hours, and finally desorbed and dried at a shelf temperature of 5-10°C for 12-16 hours to obtain freeze-dried sea cucumber protein blocks.

[0010] Preferably, in step S4, the particle size of the sea cucumber protein powder is controlled to be ≤100μm.

[0011] Preferably, in step S5, the mass ratio of ginger extract to tangerine peel extract in the natural deodorizing agent is 3:2; the gingerol content of the ginger extract is ≥5%, and the hesperidin content of the tangerine peel extract is ≥10%.

[0012] The beneficial effects of this technical solution compared to existing technologies are as follows: (1) This technical solution uses 100% pure natural sea cucumber as the only raw material to prepare calcium protein. It contains no chemical additives, preservatives, sweeteners, etc., making it green and healthy and improving practical safety. At the same time, it greatly improves the applicability of calcium protein. It can be used for children to meet the calcium needs of growth and development, and it can also be used for pregnant women to supplement calcium loss during pregnancy and postpartum and restore bone density. It can also be used for the elderly to alleviate calcium loss and improve osteoporosis. It can also be used for those with weak constitution to improve their constitution, strengthen their body and mind, and enhance their immunity. Moreover, it is reasonably priced, has a simple preparation process, and is easy to industrialize and promote.

[0013] (2) This technical solution uses natural sea cucumber to prepare calcium protein. Sea cucumber is rich in organic calcium, high-quality protein, 18 essential amino acids, sea cucumber polysaccharides, sea cucumber saponins and other nutrients. It can not only directly supplement the calcium and protein needed by the human body, but also regulate the body's immunity through bioactive substances, providing a good physiological environment for calcium absorption. The calcium protein prepared by this technical solution solves the problem of conventional calcium supplements not being absorbed. Clinical trials have shown that after taking this product for 1-4 months, human bone density is significantly improved, T value is significantly improved, and osteoporosis symptoms are effectively relieved.

[0014] (3) This technical solution adopts low-temperature freeze drying, ultra-fine pulverization and other processes to retain the nutritional components and biological activity of sea cucumber to the maximum extent. The product has good solubility, strong stability and long shelf life. The entire preparation process uses purified water and low-temperature plasma sterilization to ensure that the product meets the microbial indicators. The product is deodorized by natural plant extracts, with a mild taste and no sea cucumber smell, which greatly improves the palatability of the product. Attached Figure Description

[0015] Figure 1 A schematic flowchart of the method for preparing caloprotein provided by the present invention; Detailed Implementation The following is a reference to the accompanying drawings in the instruction manual. Figure 1 The present invention will be further described in detail below with reference to the embodiments: A highly absorbable calcium protein derived from sea cucumber is prepared by processing sea cucumber without removing its internal organs. The sea cucumbers selected are 3-5 year old raw spiny sea cucumbers that have grown naturally in the Bohai Bay and northern Yellow Sea. Their protein content is ≥60%, calcium content is ≥20mg / g, and sea cucumber polysaccharide content is ≥5%. By weight, 50 parts by weight of sea cucumber are used to prepare 1 part by weight of calcium protein.

[0016] A method for preparing a highly absorbable calcium protein derived from sea cucumber includes the following steps: S1. Raw material pretreatment: Select sea cucumber raw materials, remove impurities such as mud and shells attached to the sea cucumbers, rinse with purified water 3-5 times, and then soak in purified water at 4℃ for 48-72 hours. Change the purified water every 12 hours during the soaking period. After soaking, rinse the sea cucumbers again with purified water until the water is clear, drain the water and set aside. S2. Preliminary processing: Mix the pretreated sea cucumber with purified water at a mass ratio of 4:6. After mixing, put the mixture into a high-speed pulper and pulp for 20-30 minutes and stir evenly to obtain sea cucumber raw material pulp. Put the sea cucumber raw material pulp into a stainless steel tank for later use. S3. Low-temperature freeze drying: Pour the sea cucumber raw material slurry obtained in step S2 into a freeze drying tray, control the thickness of the sea cucumber raw material slurry to be 2-4 cm, put the freeze drying tray into a freeze dryer, first pre-freeze at a temperature of -40℃ to -30℃ for 2-3 hours, then sublimate and dry at a vacuum degree of 10-30 Pa and a shelf temperature of -20℃ to -10℃ for 48-72 hours, and finally desorb and dry at a shelf temperature of 5-10℃ for 12-16 hours to obtain freeze-dried sea cucumber protein blocks; S4. Crushing and Sterilization: The freeze-dried sea cucumber protein block obtained in step S3 is put into a vibrating ultra-micro pulverizer to crush the freeze-dried sea cucumber protein block into sea cucumber protein powder with a particle size ≤100μm. The sea cucumber protein powder is sterilized by low-temperature plasma sterilization. Specifically, the sea cucumber protein powder is transferred into the fluidized bed chamber of a vacuum low-temperature plasma sterilizer and spread evenly on a porous tray with a thickness of 0.8cm. After closing the chamber, the chamber is evacuated to 80Pa. A mixture of oxygen and nitrogen is introduced with a volume ratio of 3:7. The flow rate of the mixed gas is controlled at 12L / min. The plasma power is set to 100W and sterilization is performed for 18 minutes. After sterilization, sterile air is slowly introduced at a rate of 30Pa / s to depressurize. The sterilized sea cucumber protein powder is placed in a sterile environment to cool to room temperature to complete the sterilization. The sterilized sea cucumber protein powder is then placed in a sterile environment to cool to room temperature to obtain the storage raw material. S5. Deodorization and granulation: Take the storage raw material obtained in step S4, add a natural deodorizing agent to the storage raw material. The natural deodorizing agent is a compound of ginger extract and tangerine peel extract. The mass ratio of ginger extract to tangerine peel extract in the natural deodorizing agent is 3:2. The gingerol content of ginger extract is ≥5%, the hesperidin content of tangerine peel extract is ≥10%, and the amount of natural deodorizing agent added is 1%-3% of the mass of storage raw material. Put the storage raw material with added natural deodorizing agent into a three-dimensional mixer and mix for 15-20 minutes at a mixing speed of 20-30 r / min to obtain odorless sea cucumber protein powder. S6. Capsule filling and packaging: The odorless sea cucumber protein powder obtained in step S5 is put into a fully automatic capsule filling machine. Gastric-soluble gelatin capsules or plant-based capsules are used for filling, and the filling amount of each capsule is controlled to be 0.2-0.3g. After filling, the capsules are polished, and unqualified capsules are removed. Then, they are bottled according to specifications, and food-grade desiccant is added to each bottle before heat sealing. Finally, labels are affixed, and small, medium, and large-sized packaging boxes are used for packaging. The packaging boxes are then placed in a warehouse that meets the pharmaceutical GMP standards for storage, thus completing the preparation of calciprotein.

[0017] Example 1, A method for preparing a highly absorbable calcium protein derived from sea cucumber includes the following steps: S1. Raw material pretreatment: Select 50kg of fresh sea cucumbers that have grown naturally in the bay for 3 years, remove the mud, sand and shells attached to the sea cucumbers, rinse them 4 times with purified water, and then soak them in purified water at 4℃ for 48 hours. Change the purified water every 12 hours during the soaking period. After soaking, rinse the sea cucumbers again with purified water until the water is clear, drain the water and set aside. S2. Preliminary processing: Mix the pretreated sea cucumber with purified water at a mass ratio of 4:6. After mixing, put the mixture into a high-speed pulper and pulp at a speed of 12000r / min for 25 minutes and stir evenly to obtain sea cucumber raw material pulp. Put the sea cucumber raw material pulp into a stainless steel tank for later use. S3. Low-temperature freeze drying: Pour the sea cucumber raw material slurry obtained in step S2 into a freeze drying tray, control the thickness of the sea cucumber raw material slurry to be 3cm, put the freeze drying tray into a freeze dryer, first pre-freeze at -35℃ for 2.5 hours, then sublimate and dry at a vacuum degree of 20Pa and a shelf temperature of -15℃ for 60 hours, and finally decompose and dry at a shelf temperature of 8℃ for 14 hours to obtain freeze-dried sea cucumber protein blocks. S4. Crushing and sterilization: The freeze-dried sea cucumber protein block obtained in step S3 is put into a vibrating ultra-micro pulverizer to crush the freeze-dried sea cucumber protein block to obtain sea cucumber protein powder. The particle size of the sea cucumber protein powder is ≤100μm. The sea cucumber protein powder is sterilized by low-temperature plasma sterilization. The sterilized sea cucumber protein powder is placed in a sterile environment and cooled to room temperature to obtain storage raw material. S5. Deodorization and granulation: Take the storage raw material obtained in step S4, add a natural deodorizing agent to the storage raw material. The natural deodorizing agent is a compound of ginger extract and tangerine peel extract. The mass ratio of ginger extract to tangerine peel extract in the natural deodorizing agent is 3:2. Control the gingerol content of ginger extract to 5%, control the hesperidin content of tangerine peel extract to 10%, and add the natural deodorizing agent at 3% of the mass of the storage raw material. Put the storage raw material with added natural deodorizing agent into a three-dimensional mixer and mix at 25 r / min for 18 minutes to obtain odorless sea cucumber protein powder. S6. Capsule filling and packaging: The odorless sea cucumber protein powder obtained in step S5 is put into a fully automatic capsule filling machine. Gastric-soluble gelatin capsules or plant-based capsules are used for filling, and the filling amount of each capsule is controlled to be 0.25g. After filling, the capsules are polished, and unqualified capsules are removed. Then, they are bottled according to specifications, and food-grade desiccant is added to each bottle before heat sealing. Finally, labels are affixed, and small, medium, and large-sized packaging boxes are used for packaging. The packaging boxes are then placed in a warehouse that meets the pharmaceutical GMP standards for storage, thus completing the preparation of calciprotein.

[0018] Example 2, A method for preparing a highly absorbable calcium protein derived from sea cucumber includes the following steps: S1. Raw material pretreatment: Select 50kg of fresh sea cucumbers that have grown naturally in the bay for 3 years, remove the mud, sand and shells attached to the sea cucumbers, rinse them 5 times with purified water, and then soak them in purified water at 4℃ for 72 hours. Change the purified water every 12 hours during the soaking period. After soaking, rinse the sea cucumbers again with purified water until the water is clear, drain the water and set aside. S2. Preliminary processing: Mix the pretreated sea cucumber with purified water at a mass ratio of 4:6. After mixing, put the mixture into a high-speed pulper and pulp at a speed of 12000r / min for 25 minutes and stir evenly to obtain sea cucumber raw material pulp. Put the sea cucumber raw material pulp into a stainless steel tank for later use. S3. Low-temperature freeze drying: Pour the sea cucumber raw material slurry obtained in step S2 into a freeze drying tray, control the thickness of the sea cucumber raw material slurry to be 2cm, put the freeze drying tray into a freeze dryer, first pre-freeze at -40℃ for 2 hours, then sublimate and dry at a vacuum of 10Pa and a shelf temperature of -20℃ for 72 hours, and finally decompose and dry at a shelf temperature of 10℃ for 12 hours to obtain freeze-dried sea cucumber protein blocks. S4. Crushing and sterilization: The freeze-dried sea cucumber protein block obtained in step S3 is put into a vibrating ultra-micro pulverizer to crush the freeze-dried sea cucumber protein block to obtain sea cucumber protein powder. The particle size of the sea cucumber protein powder is ≤100μm. The sea cucumber protein powder is sterilized by low-temperature plasma sterilization. The sterilized sea cucumber protein powder is placed in a sterile environment and cooled to room temperature to obtain storage raw material. S5. Deodorization and granulation: Take the storage raw material obtained in step S4, add a natural deodorizing agent to the storage raw material. The natural deodorizing agent is a compound of ginger extract and tangerine peel extract. The mass ratio of ginger extract to tangerine peel extract in the natural deodorizing agent is 3:2. Control the gingerol content of ginger extract to 6%, control the hesperidin content of tangerine peel extract to 12%, and add the natural deodorizing agent at 3% of the mass of the storage raw material. Put the storage raw material with added natural deodorizing agent into a three-dimensional mixer and mix at 25 r / min for 18 minutes to obtain odorless sea cucumber protein powder. S6. Capsule filling and packaging: The odorless sea cucumber protein powder obtained in step S5 is put into a fully automatic capsule filling machine. Gastric-soluble gelatin capsules or plant-based capsules are used for filling, and the filling amount of each capsule is controlled to be 0.25g. After filling, the capsules are polished, and unqualified capsules are removed. Then, they are bottled according to specifications, and food-grade desiccant is added to each bottle before heat sealing. Finally, labels are affixed, and small, medium, and large-sized packaging boxes are used for packaging. The packaging boxes are then placed in a warehouse that meets the pharmaceutical GMP standards for storage, thus completing the preparation of calciprotein.

[0019] Clinical trials, Sixty patients with osteoporosis or osteopenia were selected, including 10 children, 15 pregnant women, 25 elderly people, and 10 people with weak constitutions. These patients took two calcium protein capsules prepared according to Example 1 twice daily for four months. The patients' recovery was then assessed based on changes in their T-scores. The data are shown in the table below. As shown in the table above, the product of this invention can significantly improve bone density in different populations, with a significant improvement in T-score, and no adverse reactions, proving its safety and effectiveness.

[0020] In bone mineral density testing and clinical trial data of this invention, the T-score is a core indicator for assessing whether bone mineral density is normal and determining the degree of osteoporosis. The T-score is the standard deviation (SD) difference calculated by comparing the bone mineral density of the tested individual with the average bone mineral density of healthy young adults (20-30 years old, peak bone mineral density group). The formula can be simplified as follows: T-score = (Bone mineral density of the subject - average bone mineral density of young adults) / standard deviation of bone mineral density of young adults; by comparing the patient's T-score with the standard published by the World Health Organization (WHO), it can be determined whether the patient has normal bone mass, osteopenia, or osteoporosis; this is explained here.

[0021] The above descriptions are merely embodiments of the present invention, and common knowledge such as specific technical solutions and / or characteristics are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solutions of the present invention, and these should also be considered within the scope of protection of the present invention. These modifications and improvements will not affect the effectiveness of the implementation of the present invention or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A sea cucumber-derived highly absorbable calcium protein, characterized in that, The calcium protein is prepared from sea cucumber. The sea cucumber is not viscerated during the preparation of calcium protein. The sea cucumber is selected from fresh sea cucumbers that have grown naturally for 3-5 years. The protein content is ≥60%, the calcium content is ≥20mg / g, and the sea cucumber polysaccharide content is ≥5%.

2. The sea cucumber-derived highly absorbable calcium protein as described in claim 1, characterized in that, By weight, 50 parts by weight of sea cucumber can prepare 1 part by weight of calcium protein.

3. A method for preparing a sea cucumber-derived highly absorbable calcium protein, used to prepare the calcium protein according to claim 1 or 2, characterized in that, Includes the following steps: S1. Raw material pretreatment: Select sea cucumber raw materials, remove impurities such as mud and shells attached to the sea cucumbers, rinse with purified water 3-5 times, and then soak in purified water for 48-72 hours. After soaking, rinse the sea cucumbers again with purified water until the water is clear, drain the water and set aside. S2. Preliminary processing: Mix the pretreated sea cucumber with purified water at a mass ratio of 4:

6. After mixing, put the mixture into a high-speed pulper and pulp for 20-30 minutes and stir evenly to obtain sea cucumber raw material pulp. Put the sea cucumber raw material pulp into a stainless steel tank for later use. S3. Low-temperature freeze drying: Pour the sea cucumber raw material slurry obtained in step S2 into a freeze drying tray, control the thickness of the sea cucumber raw material slurry to be 2-4cm, put the freeze drying tray into a freeze dryer, and freeze dry at low temperature to obtain freeze-dried sea cucumber protein blocks. S4. Crushing and sterilization: The freeze-dried sea cucumber protein block obtained in step S3 is put into a vibrating ultra-micro pulverizer to crush the freeze-dried sea cucumber protein block into sea cucumber protein powder. The sea cucumber protein powder is sterilized by low-temperature plasma sterilization. The sterilized sea cucumber protein powder is placed in a sterile environment and cooled to room temperature to obtain storage raw material. S5. Deodorization and granulation: Take the storage raw material obtained in step S4, add a natural deodorizing agent to the storage raw material. The natural deodorizing agent is a compound of ginger extract and tangerine peel extract. The amount of natural deodorizing agent added is 1%-3% of the mass of the storage raw material. Put the storage raw material with added natural deodorizing agent into a three-dimensional mixer and mix for 15-20 minutes at a mixing speed of 20-30 r / min to obtain odorless sea cucumber protein powder. S6. Capsule filling and packaging: The odorless sea cucumber protein powder obtained in step S5 is put into a fully automatic capsule filling machine. Gastric-soluble gelatin capsules or plant-based capsules are used for filling, and the filling amount of each capsule is controlled to be 0.2-0.3g. After filling, the capsules are polished, and unqualified capsules are removed. Then, they are bottled according to specifications, and food-grade desiccant is added to each bottle before heat sealing. Finally, labels are affixed, and small, medium, and large-sized packaging boxes are used for packaging. The packaging boxes are then placed in a warehouse that meets the pharmaceutical GMP standards for storage, thus completing the preparation of calciprotein.

4. The method for preparing a sea cucumber-derived highly absorbable calcium protein as described in claim 3, characterized in that, In step S1, the temperature of the purified water used to soak the sea cucumbers is controlled at 4°C.

5. The method for preparing a sea cucumber-derived highly absorbable calcium protein as described in claim 3, characterized in that, In step S1, the purified water is changed every 12 hours during the soaking of sea cucumbers.

6. The method for preparing a sea cucumber-derived highly absorbable calcium protein as described in claim 3, characterized in that, In step S3, after placing the freeze-drying tray into the freeze dryer, it is first pre-frozen at a temperature of -40℃ to -30℃ for 2-3 hours, then sublimated and dried at a vacuum of 10-30Pa and a shelf temperature of -20℃ to -10℃ for 48-72 hours, and finally desorbed and dried at a shelf temperature of 5-10℃ for 12-16 hours to obtain freeze-dried sea cucumber protein blocks.

7. The method for preparing a sea cucumber-derived highly absorbable calcium protein as described in claim 3, characterized in that, In step S4, the particle size of the sea cucumber protein powder is controlled to be ≤100μm.

8. The method for preparing a sea cucumber-derived highly absorbable calcium protein as described in claim 3, characterized in that, In step S5, the mass ratio of ginger extract to tangerine peel extract in the natural deodorizing agent is 3:2; the gingerol content of the ginger extract is ≥5%, and the hesperidin content of the tangerine peel extract is ≥10%.

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