Method for determining carvacol content in compound fertilizer

By employing a dual-path detection system, including GC low-temperature ultrasonic extraction and silica gel column purification, and HPLC methanol-water system purification, the matrix interference problem in the detection of carvacrol in compound fertilizers was solved, achieving high-precision and stable quantitative analysis.

CN121784200BActive Publication Date: 2026-06-09SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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Patent Information

Application Number
CN202610267735.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2026-03-06
Publication Date
2026-06-09
Estimated Expiration
2046-03-06

AI Technical Summary

Technical Problem

Existing technologies for detecting carvacrol content in compound fertilizers suffer from incomplete matrix purification, leading to impurity peaks interfering with the target analyte, column blockage, large deviations in quantitative results, and severe loss of volatile components, especially in GC methods where the loss rate is as high as 18%-32%.

Method used

A dual-path detection system was adopted. The GC path used low-temperature ultrasonic extraction and silica column polarization purification, while the HPLC path used a methanol-water dynamic solvent system and C18 column for synergistic purification. The silica solid-phase extraction and C18 solid-phase extraction columns were used for gas chromatography and liquid chromatography, respectively, to eliminate interference from high-salt and high-humic acid matrices.

Benefits of technology

It achieves high-precision quantification of carvacrol in compound fertilizer (RSD≤3.5%), improves the recovery rate of GC method, avoids column blockage of HPLC method, is accurate within the quantitative range of 10-1000 mg/kg, reduces interference from impurity peaks, and improves the stability and accuracy of detection.

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Abstract

The application discloses a method for determining the content of carvacol in compound fertilizer, and the method comprises gas phase determination and liquid chromatography, and the gas chromatography method for determining the content of carvacol in compound fertilizer comprises the following steps: S1, sample pretreatment; S2, matrix purification; S3, chromatographic analysis; and S4, quantitative calculation; in the application, a silica gel SPE column is used to replace a traditional diatomite column in the GC method, the polarity impurity adsorption is enhanced through ethyl acetate-hexane gradient activation, sodium chloride salting-out and EDTA complex metal ions are introduced in the HPLC method, the number of miscellaneous peaks in the GC chromatogram is reduced, the separation degree of the carvacol peak is improved, and the service life of the HPLC column is prolonged; the GC method is operated under the conditions of temperature control ultrasonic at 35±2 DEG C and full light avoidance; the HPLC method adopts room temperature methanol-water system extraction, and the recovery rate of the GC method is improved; the HPLC method avoids the 60 DEG C concentration requirement of acetonitrile extraction, and the light stability is improved.
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