Liver cancer cell collector

Abstract

The invention relates to the technical field of hepatoma cell collection, in particular to a hepatoma cell collector which comprises a tray and a sealing cover meshed with the tray in a matched mode, a plurality of sets of collecting pipes are arranged on the upper side of the tray, and the collecting pipes penetrate through a plurality of sets of clamping grooves formed in the top face of the tray. A suction head used in cooperation with the collection pipe is arranged on the lower side of the collection pipe, a piston rod is arranged in the collection pipe, and a refrigeration part used for keeping the low temperature of the collection pipe is arranged on the outer side of the collection pipe; and the two groups of sealing assemblies are arranged in the collecting pipe and are used for keeping the interior of the collecting pipe sealed. Through the sealing assembly, after hepatoma cells are collected into the collection tube, the interior of the collection tube is sealed through fitting of two semicircular sealing blocks under the action of a first elastic piece, so that external infection can be avoided during storage and separation after collection, and meanwhile, only one collection tube is used in the process; and cross infection caused by conveying of a plurality of pipe fittings is avoided.

Description

Technical Field

[0001] This invention relates to the field of liver cancer cell collection technology, specifically to a liver cancer cell collector. Background Technology

[0002] Liver cancer, or malignant tumor of the liver, can be divided into two main categories: primary and secondary. Primary malignant liver tumors originate from the epithelial or mesenchymal tissue of the liver. In the treatment of liver cancer, surgical resection is usually required. Before surgery, liver cancer cells need to be collected, isolated, and studied to analyze the condition.

[0003] However, in the process of collecting liver cancer cells, a collector is needed for storage, transportation and separation. Currently, most collection processes involve extracting liver cancer cells through an aspiration device and then transporting them to a test tube. The collection and storage effect is not good. During this process, the cells may be infected by viruses in the air, resulting in poor purity. Furthermore, the cell survival environment is poor, and prolonged storage can easily cause stem cells to lose their activity. Injecting reagents to maintain their activity is too troublesome. Summary of the Invention

[0004] To address the aforementioned shortcomings of existing technologies, this invention provides a liver cancer cell collector that effectively solves the problems of poor sealing during liver cancer cell collection, which can easily lead to infection, and the high requirements for storage environment.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] This invention provides a liver cancer cell collector, comprising a tray and a sealing cap that engages with the tray. Multiple sets of collection tubes are arranged on the upper side of the tray, and each set of collection tubes passes through multiple slots opened on the top surface of the tray. A suction head is arranged on the lower side of each collection tube for use with it. A piston rod is provided inside each collection tube, and a cooling element for maintaining the low temperature of the collection tube is provided on the outer side of the collection tube. Two sealing assemblies are arranged inside the collection tubes to maintain a seal. Each sealing assembly includes two sets of semi-circular sealing blocks. A first elastic element and two connecting rods are fixedly connected to one side of each of the two sealing blocks. The connecting rods on the same side are connected by a sliding rod. The sliding rod is fixed to the outer surface of the collection tube by a fixing element. Grooves are opened on opposite sides of each sealing block. Multiple sets of second elastic elements are fixedly connected inside each groove. A sealing plate is fixedly connected to the second elastic element located inside the same groove.

[0007] Furthermore, the inside of the collection tube is provided with a sliding groove, the sealing block and the connecting rod slide in cooperation with the sliding groove, and one end of the first elastic element is fixedly connected to one side of the inner wall of the sliding groove.

[0008] Furthermore, the top surface of the chute is provided with multiple sets of sliding grooves, and the sliding rod slides in cooperation with the sliding grooves and extends to the outside of the collection tube.

[0009] Furthermore, the sealing assembly also includes an annular sealing element that contacts and cooperates with the sealing block. One side of the annular sealing element is fixedly connected to multiple sets of third elastic elements, and the other end of the third elastic element is fixedly connected to a sealing groove opened on one side of the slide groove. The two sets of sealing grooves are respectively located on the opposite sides of the sealing blocks on the upper and lower sides.

[0010] Furthermore, the length of the sealing plate is greater than the inner diameter of the collection tube, and a magnetic suction element is provided on one side of the sealing plate. The magnetic suction elements on the two sets of sealing plates located on the same horizontal plane cooperate to attract and fix them.

[0011] Furthermore, the fastener includes an elastic buckle disposed inside the sliding rod.

[0012] Furthermore, the cooling component includes a liquid inlet pipe disposed inside the collection tube, the liquid inlet pipe being connected to a cold liquid tank opened inside the collection tube, and a sealing plug that cooperates with the liquid inlet pipe being disposed inside the liquid inlet pipe, and the sealing plug being made of rubber material.

[0013] The technical solution provided by this invention has the following advantages compared with the known prior art:

[0014] 1. The present invention uses a sealing component to seal the inside of the collection tube after liver cancer cells are collected. Two semi-circular sealing blocks fit together under the action of the first elastic element to seal the inside of the collection tube. This prevents external infection during storage and separation after collection. At the same time, only one collection tube is used in this process, avoiding cross-infection caused by transporting multiple tubes.

[0015] 2. This invention provides two sealing plates that are attracted and fixed by magnetic attraction, and a second elastic element, thereby increasing the sealing performance during the bonding process of the two semi-circular sealing blocks and enhancing the sealing effect in the horizontal direction. At the same time, an annular sealing element is provided in conjunction with a third elastic element, which allows the annular sealing element to fit tightly against the sealing block, thereby enhancing the sealing effect in the vertical direction.

[0016] 3. By incorporating a cooling component, this invention maintains a low temperature inside the collection tube, thereby preserving the activity of liver cancer cells and providing a favorable storage environment. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without any creative effort.

[0018] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0019] Figure 2 This is a partial structural diagram of the present invention;

[0020] Figure 3 This is a schematic diagram illustrating the separation of the collection tube and the tray in this invention;

[0021] Figure 4 This is a schematic cross-sectional view of the acquisition tube in this invention;

[0022] Figure 5 In this invention Figure 4 Enlarged schematic diagram of the structure at point A in the middle;

[0023] Figure 6 This is a schematic cross-sectional view of the sealing assembly in this invention;

[0024] Figure 7 In this invention Figure 6 Enlarged schematic diagram of the structure at point B;

[0025] Figure 8 This is a schematic diagram showing the separation of the sealing assembly and the collection tube in this invention;

[0026] Figure 9 In this invention Figure 8 Enlarged schematic diagram of the structure at point C.

[0027] The labels in the diagram represent: 1. Tray; 101. Slot; 2. Sealing cap; 3. Collection tube; 301. Slide groove; 302. Sliding groove; 303. Sealing groove; 4. Suction head; 5. Piston rod; 6. Refrigeration component; 601. Liquid inlet pipe; 602. Cold liquid tank; 603. Sealing plug; 7. Sealing assembly; 701. First elastic element; 702. Sealing block; 7021. Groove; 703. Connecting rod; 704. Sliding rod; 705. Elastic buckle; 8. Second elastic element; 801. Sealing plate; 9. Annular seal; 901. Third elastic element. Detailed Implementation

[0028] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0029] The present invention will be further described below with reference to embodiments.

[0030] Example: A liver cancer cell harvester, such as Figures 1-9 As shown, the device includes a tray 1 and a sealing cap 2 that engages with it. By setting the tray 1 and the sealing cap 2 together, the overall sealing is increased during transportation, and the damage to the collection tubes 3 caused by collisions during transportation can be reduced. Multiple sets of collection tubes 3 are set on the upper side of the tray 1. By setting multiple sets of collection tubes 3, multiple sets of liver cancer cells can be collected at the same time, which facilitates comparison during research and analysis. The collection tubes 3 pass through multiple sets of slots 101 opened on the top surface of the tray 1. A suction head 4 is set on the lower side of the collection tube 3 for use with it. The cells are suctioned by the suction head 4 without affecting other collection tubes 3. Each collection tube 3 is equipped with a piston rod 5. The suction process of the piston rod 5 creates a negative pressure state inside the collection tube 3, allowing the cells to enter the collection tube 3. A cooling element 6 is set on the outside of the collection tube 3 to maintain the low temperature of the collection tube 3. The cooling element 6 can maintain the low temperature environment around the collection tube 3 and maintain the environment of liver cancer cells.

[0031] Two sets of sealing components 7 are disposed inside the collection tube 3 to maintain the seal inside the collection tube 3. Each sealing component 7 includes two sets of semi-circular sealing blocks 702. The sealing blocks 702 cover the inner diameter of the collection tube 3, and the combined diameter of the two sealing blocks 702 is larger than the inner diameter of the collection tube 3. A first elastic element 701 and two sets of connecting rods 703 are fixedly connected to one side of each of the two sets of sealing blocks 702. The sealing blocks 702 fit together under the action of the first elastic element 701. The connecting rods 703 on the same side are connected by a sliding rod 704. Moving the sliding rod 704 moves the sealing blocks 702. The sliding rod 704 is fixed to the outer surface of the collection tube 3 by a fixing component. During the collection process, the sealing blocks 702 are fixed by the fixing component (e.g., ...). Figure 4As shown), the opposite surfaces of the sealing block 702 are provided with grooves 7021. Multiple sets of second elastic elements 8 are fixedly connected inside the grooves 7021. The second elastic elements 8 exert a force on the sealing plate 801. The second elastic elements 8 located inside the same groove 7021 are fixedly connected to the sealing plate 801. The sealing plate 801 can increase the sealing effect in the horizontal direction.

[0032] The sealing assembly 7 also includes an annular seal 9 that contacts and engages with the sealing block 702. Multiple sets of third elastic elements 901 are fixedly connected to one side of the annular seal 9. Under the force of the third elastic elements 901, the annular seal 9 fits against the sealing block 702, increasing the sealing performance in the vertical direction. The other end of the third elastic element 901 is fixedly connected to a sealing groove 303 opened on one side of the slide groove 301. The two sets of sealing grooves 303 are located on the opposite sides of the sealing blocks 702 on the upper and lower sides, respectively, so that the inside of the collection tube 3 can be sealed in the upper and lower directions respectively.

[0033] The inside of the collection tube 3 is provided with a sliding groove 301. The sealing block 702 and the connecting rod 703 slide in the sliding groove 301 to limit the sealing block 702 and the connecting rod 703 in the horizontal direction. One end of the first elastic member 701 is fixedly connected to one side of the inner wall of the sliding groove 301.

[0034] The top surface of the slide groove 301 is provided with multiple sets of sliding grooves 302, and the sliding rod 704 slides in cooperation with the sliding groove 302. The sliding rod 704 can be used to operate the internal sealing block 702 and extends to the outside of the collection tube 3.

[0035] Among them, the length of the sealing plate 801 is greater than the inner diameter of the collection tube 3, and a magnetic suction component is provided on one side of the sealing plate 801. The magnetic suction components on the two sets of sealing plates 801 located on the same horizontal plane cooperate to attract and fix them. By fixing them with the magnetic suction components, the force between the two sealing plates 801 can be increased, so that the two can fit tightly together.

[0036] The fastener includes an elastic buckle 705 disposed inside the sliding rod 704, which facilitates fixation.

[0037] The refrigeration component 6 includes a liquid inlet pipe 601 installed inside the collection pipe 3. The liquid inlet pipe 601 delivers refrigerant to the interior of the cold liquid tank 602. The liquid inlet pipe 601 is connected to the cold liquid tank 602 inside the collection pipe 3. A sealing plug 603 is installed inside the liquid inlet pipe 601 to cooperate with it. The sealing plug 603 can increase the sealing performance and prevent the refrigerant from spilling out during transportation. The sealing plug 603 is made of rubber.

[0038] Specifically, during the collection of liver cancer cells using the aforementioned device, the suction head 4 is inserted into the end of one of the collection tubes 3, and then the piston rod 5 is pulled out. At this time, the liver cancer cells enter the interior of the collection tube 3. The piston rod 5 is pulled out to the upper side, and the tray 1 is inverted. Then, the elastic buckle 705 is pulled out from the upper side. At this time, under the action of the first elastic element 701, the sealing block 702 slides and fits inside the groove 301. At this time, the two sealing plates 801 are tightly fitted under the action of the magnetic element and the second elastic element 8, and the annular sealing element 9 is fitted by the third elastic element 901. The function is to fit tightly with the sealing block 702, and then repeat this operation to fit the lower sealing block 702. Finally, remove the piston rod 5. At this time, the inside of the collection tube 3 can be sealed, reducing the impact of the outside world during storage and transportation. After the processing is completed, the sealing plug 603 is pulled out from the inside of the liquid inlet pipe 601, and the refrigerant is poured into the inside of the cold liquid tank 602 through the connecting pipe. Then, the sealing plug 603 is inserted into the liquid inlet pipe 601 for sealing. After multiple collection tubes 3 have completed collection, the sealing cover 2 is screwed tightly onto the tray 1, so as to maintain the activity of liver cancer cells and the internal sealing.

[0039] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the protection scope of the technical solutions of the embodiments of the present invention.

Claims

1. A liver cancer cell collector, comprising a tray (1) and a sealing cap (2) engaging therewith, characterized in that, The upper side of the tray (1) is provided with multiple sets of collection tubes (3), and the multiple sets of collection tubes (3) pass through multiple sets of slots (101) opened on the top surface of the tray (1). The lower side of the collection tube (3) is provided with a suction head (4) for use therewith. The inside of the collection tube (3) is provided with a piston rod (5). The outside of the collection tube (3) is provided with a cooling component (6) for keeping the collection tube (3) at a low temperature. Two sets of sealing components (7) are set inside the collection tube (3) to keep the inside of the collection tube (3) sealed. The sealing components (7) include two sets of semi-circular sealing blocks (702). One side of each of the two sets of sealing blocks (702) is fixedly connected to a first elastic element (701) and two sets of connecting rods (703). The connecting rods (703) on the same side are connected by a sliding rod (704). The sliding rod (704) is fixed to the outer surface of the collection tube (3) by a fastener. The opposite sides of the sealing blocks (702) are provided with grooves (7021). Multiple sets of second elastic elements (8) are fixedly connected inside each groove (7021). The second elastic element (8) located inside the same groove (7021) is fixedly connected to a sealing plate (801).

2. The liver cancer cell collector according to claim 1, characterized in that, The inside of the collection tube (3) is provided with a sliding groove (301), the sealing block (702) and the connecting rod (703) are slidably engaged with the sliding groove (301), and one end of the first elastic member (701) is fixedly connected to one side of the inner wall of the sliding groove (301).

3. A liver cancer cell collector according to claim 2, characterized in that, The top surface of the chute (301) is provided with multiple sets of sliding grooves (302), and the sliding rod (704) slides in cooperation with the sliding groove (302) and extends to the outside of the collection tube (3).

4. A liver cancer cell collector according to claim 1, characterized in that, The sealing assembly (7) further includes an annular seal (9) that contacts and engages with the sealing block (702). One side of the annular seal (9) is fixedly connected to multiple sets of third elastic elements (901). The other end of the third elastic element (901) is fixedly connected to a sealing groove (303) opened on one side of the slide (301), and the two sets of sealing grooves (303) are respectively located on the opposite sides of the sealing block (702) on the upper and lower sides.

5. A liver cancer cell collector according to claim 1, characterized in that, The length of the sealing plate (801) is greater than the inner diameter of the collection tube (3), and a magnetic suction element is provided on one side of the sealing plate (801). The magnetic suction elements on the two sets of sealing plates (801) located on the same horizontal plane cooperate to attract and fix them.

6. A liver cancer cell collector according to claim 1, characterized in that, The fastener includes an elastic buckle (705) disposed inside the sliding rod (704).

7. A liver cancer cell collector according to claim 1, characterized in that, The refrigeration component (6) includes an inlet pipe (601) inside the collection pipe (3), the inlet pipe (601) is connected to a cold liquid tank (602) inside the collection pipe (3), and a sealing plug (603) is provided inside the inlet pipe (601) to cooperate with it, and the sealing plug (603) is made of rubber.

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