A pesticide composition containing a chordophone regulator and its use

Abstract

The present application relates to synergistic compositions containing a biologically effective amount of compound I and a compound II, which is one of flonicamid, pyrifluquinazon, verbutin, pymetrozine or bisultap, and methods for using them to control pests in agronomic and non-agronomic environments. The compound I and the compound II have synergistic effects, which can improve the pest control effect, reduce the amount of application, reduce the frequency of use, and reduce the cost of use.

Description

Technical Field

[0001] This invention belongs to the technical field of pesticide compound formulation, specifically relating to a biologically effective amount of compound I and at least one string-regulating insecticide (fluoxetine, pyridaben, fenpyroxetine, pymetrozine, or difenoconazole), and methods for using them to control pests in agricultural and non-agricultural environments. Background Technology

[0002] In recent years, the occurrence of various crop pests has shown a trend of increasing severity year by year, with the affected area growing annually, causing enormous damage to crops and leading to problems such as reduced crop yields and increased agricultural production costs. To improve pest control, farmers often increase pesticide dosage or arbitrarily mix pesticides. This unscientific use of pesticides not only fails to achieve synergistic effects but also leads to pesticide waste, excessive residues, environmental pollution, and the development of pesticide resistance in pests. Therefore, there is an urgent need to develop a synergistic combination for pests that can broaden the range of target pests, reduce pesticide dosage, and delay the development of resistance.

[0003] In actual agricultural production, the long-term, continuous use of the same pesticide quickly leads to pesticide resistance in pests, resulting in reduced efficacy, increased pesticide usage, and exacerbated pesticide residues in agricultural products and damage to the ecological environment. Combining insecticides with completely different mechanisms of action is an effective way to delay the development of pesticide resistance, broaden the pesticide spectrum, extend the pesticide's lifespan, and reduce pesticide usage. However, determining how to combine the pesticides to achieve synergistic rather than antagonistic effects remains a significant challenge. Summary of the Invention

[0004] Patent document CN119101038B discloses compound I, cyclopyrazosulfuron, with the chemical name N-(cyclopropylmethyl)-5-methyl-1-(3-methylbutane-2-yl)-N-(pyridazin-4-yl)-1H-pyrazole-4-carboxamide. It has broad-spectrum insecticidal activity, especially effective against pests such as Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera, and mites.

[0005] To overcome the shortcomings of the prior art, the present invention provides a composition comprising compound I and compound II; The compound I is cyclopyridamole, with the chemical name N-(cyclopropylmethyl)-5-methyl-1-(3-methylbutan-2-yl)-N-(pyridazin-4-yl)-1H-pyrazole-4-carboxamide; Compound II is a string-tuning insecticide.

[0006] According to embodiments of the present invention, compound II is selected from any one, two, or more of the following: flonicamid (N-cyanomethyl-4-(trifluoromethyl)nicotinamide), pyridinium quinazoline (1-acetyl-6-(1,1,1,2,3,3,3-heptafluoroprop-2-yl)-3-(pyridin-3-ylmethylamino)-4H-benzopyrimidin-2-one), pymetrozine (N-ethyl-5-methyl-1-(3-methylbut-2-yl)-N-pyridazin-4-ylpyrazole-4-carboxamide), and pymetrozine (4,5-dihydro-6-methyl) -4-(3-pyridinylmethyleneamino)-1,2,4-3(2H)-one, and dipropionyl ester ([(3S,4R,4aR,6S,6aS,12R,12aS,12bS)-3-(cyclopropylcarbonyloxy)-1,3,4,4a,5,6,6a,12,12a,12b-decahydro-6,12-dihydroxy-4,6a,12b-trimethyl-11-oxa-9-(3-pyridyl)-2H,11H-benzo[f]pyran[4,3-b]chroman-4-yl]cyclopropanecarboxylic acid methyl ester).

[0007] According to an embodiment of the present invention, compound I was prepared by the method of Example 1 in patent document CN119101038B, the entire contents of which are incorporated herein by reference.

[0008] According to an embodiment of the present invention, the mass ratio of compound I to compound II is 80~1:1~80.

[0009] According to an embodiment of the present invention, the mass ratio of compound I to compound II is 50~1:1~50.

[0010] According to a preferred embodiment of the present invention, the mass ratio of compound I to compound II is 10~1:1~10.

[0011] As an example, the mass ratio of compound I to compound II is 50:1, 40:1, 30:1, 20:1, 10:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:10, 1:20, 1:30, 1:40, 1:50 or any value (or any range) between the above values.

[0012] According to an embodiment of the present invention, the total mass of compound I and compound II in the composition is 1-80%, preferably 2-70%, or 5-65%; 10-60%, for example 1%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45% or 50%, based on the total weight of the composition as 100%.

[0013] According to an embodiment of the present invention, the composition comprises compound I and compound II; compound I is cyclopyridamole, and compound II is selected from any of the following: flonicamid, pyridazolin, cyclopyridamole, pymetrozine, and diproteil; Preferably, the mass ratio of compound I to compound II is 80:1 to 1:80, more preferably 50:1 to 1:50, and even more preferably 10:1 to 1:50, 50:1 to 1:10, 10:1 to 1:10, 50:1 to 1:10, or 5:1 to 1:50; for example, 50:1, 40:1, 30:1, 20:1, 10:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:10, 1:20, 1:30, 1:40, 1:50, or any value (or any range) between the above values. Preferably, the composition is used to control pests such as Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera, and mites; preferably, the composition is used to control aphids (e.g., cotton aphids), brown planthoppers (e.g., rice brown planthoppers), and whiteflies (e.g., tomato whiteflies).

[0014] According to an embodiment of the present invention, the composition comprises compound I and compound II; compound I is cyclopyridamole, and compound II is flonicamid; Preferably, the mass ratio of compound I to compound II is 80:1 to 1:80, more preferably 50:1 to 1:50, and even more preferably 10:1 to 1:50; for example, 10:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:10, 1:20, 1:30, 1:40, 1:50, or any value (or any range) between the above values. Preferably, the composition is used to control Hemiptera pests; preferably, the composition is used to control aphids (e.g., cotton aphids).

[0015] According to an embodiment of the present invention, the composition comprises compound I and compound II; compound I is cyclophosphamide, and compound II is pyridinequinazoline; Preferably, the mass ratio of compound I to compound II is 80:1 to 1:80, more preferably 50:1 to 1:50, and even more preferably 50:1 to 1:10; for example, 50:1, 40:1, 30:1, 20:1, 10:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:10 or any value (or any range) between the above values; Preferably, the composition is used to control Hemiptera pests; preferably, the composition is used to control aphids (e.g., cotton aphids).

[0016] According to an embodiment of the present invention, the composition comprises compound I and compound II; compound I is cyclopyridamole, and compound II is cyclopyridamole. Preferably, the mass ratio of compound I to compound II is 80:1 to 1:80, more preferably 50:1 to 1:50, and even more preferably 10:1 to 1:10; for example, 10:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:10 or any value (or any range) between the above values. Preferably, the composition is used to control Hemiptera pests; preferably, the composition is used to control aphids (e.g., cotton aphids).

[0017] According to an embodiment of the present invention, the composition comprises compound I and compound II; compound I is cyclopyridamole, and compound II is pymetrozine; Preferably, the mass ratio of compound I to compound II is 80:1 to 1:80, more preferably 50:1 to 1:50, and even more preferably 50:1 to 1:10; for example, 10:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:10 or any value (or any range) between the above values. Preferably, the composition is used to control Hemiptera pests; preferably, the composition is used to control brown planthoppers (e.g., rice brown planthoppers).

[0018] According to an embodiment of the present invention, the composition comprises compound I and compound II; compound I is cyclopyridamole, and compound II is diproteil; Preferably, the mass ratio of compound I to compound II is 80:1 to 1:80, more preferably 50:1 to 1:50, and even more preferably 5:1 to 1:50; for example, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:10, 1:20, 1:30, 1:40, 1:50, or any value (or any range) between the above values. Preferably, the composition is used to control Hemiptera pests; preferably, the composition is used to control whiteflies (e.g., tomato whiteflies).

[0019] According to embodiments of the present invention, the composition can be prepared as a liquid formulation or a solid formulation.

[0020] According to embodiments of the present invention, the content range of the effective active ingredient in the formulation varies depending on the type of formulation. Generally, liquid formulations contain 1% to 60% of the effective active ingredient by weight, preferably 5% to 50%; solid formulations contain 5% to 80% of the effective active ingredient by weight, preferably 10% to 70%.

[0021] According to embodiments of the present invention, the composition further includes at least one of water (e.g., deionized water), organic solvent, emulsifier, dispersant, wetting agent, thickener, defoamer, stabilizer, binder, disintegrant, antifreeze, anticaking agent, suspending agent, film-forming agent, preservative, colorant, polymeric capsule wall material, pH adjuster, or filler.

[0022] According to embodiments of the present invention, the composition can be diluted or used directly by the user before use. Its formulation can be prepared by processing methods known to those skilled in the art, i.e., by mixing the active ingredient with one or more of the following: deionized water, organic solvents, emulsifiers, dispersants, wetting agents, thickeners, defoamers, stabilizers, binders, disintegrants, antifreeze agents, anti-caking agents, suspending agents, film-forming agents, preservatives, colorants, polymeric capsule wall materials, pH adjusters, or fillers, to prepare the composition.

[0023] According to the formulation provided by the present invention, the composition can be formulated into various dosage forms, preferably including wettable powder, water-dispersible granules, suspension concentrate, water emulsion, suspension seed coating agent, microcapsule suspension, microcapsule suspension-suspension agent, emulsifiable concentrate, microemulsion, dispersible liquid, and granules.

[0024] According to an embodiment of the present invention, when the composition is formulated into a wettable powder, it comprises the following components and contents: compound I 0.1%~80%, compound II 0.1%~75%, dispersant 0%~10%, wetting agent 0%~8%, and the balance being filler.

[0025] Preferably, the compound I in the composition is 2%~70%, 5%~65%, 10%~60%, 15%~50%, etc.; Compound II is present in concentrations of 2%–70%, 5%–65%, 10%–60%, 15%–50%, etc. The dispersant concentration is 1%~8%, 2%~7%, 3-6%, or 4%~5%; The wetting agent is 1%~8%, 2%~7%, 3-6%, or 4%~5%.

[0026] According to an embodiment of the present invention, when the composition is formulated into water-dispersible granules, it comprises the following components and contents: compound I 0.1%~80%, compound II 0.1%~75%, dispersant 0%~10%, wetting agent 0%~16%, disintegrant 0%~20%, and the balance being filler.

[0027] Preferably, the compound I in the composition is 2%~70%, 5%~65%, 10%~60%, 15%~50%, etc.; Compound II is present in concentrations of 2%–70%, 5%–65%, 10%–60%, 15%–50%, etc. The dispersant concentration is 1%~8%, 2%~7%, 3-6%, or 4%~5%; The wetting agent is 1%~8%, 2%~7%, 3-6% or 4%~5%; The disintegrant is 1%~18%, 2%~16%, 3-15%, 4%~12%, 5%~10%, or 6-8%.

[0028] According to an embodiment of the present invention, when the composition is made into a suspension, it comprises the following components and contents: compound I 0.1%~50%, compound II 0.1%~50%, dispersant 0%~5%, wetting agent 0%~8%, thickener 0%~0.3%, antifreeze agent 0%~5%, and the balance being deionized water.

[0029] Preferably, compound I in the composition is 2%~45%, 5%~40%, 10%~35%, 15%~30%, 20%~25%, etc.; Compound II is present in concentrations of 2%–45%, 5%–40%, 10%–35%, 15%–30%, and 20%–25%, etc. The dispersant is 1%~4% or 2%~3%; The wetting agent is 1%~8%, 2%~7%, 3-6% or 4%~5%; The thickener content is 0.1%~0.25%; Antifreeze 1%~4% or 2%~3%.

[0030] According to an embodiment of the present invention, when the composition is formulated into an aqueous emulsion, it comprises the following components and contents: compound I 0.1%~50%, compound II 0.1%~50%, organic solvent 0%~95%, emulsifier 0%~10%, antifreeze 0%~5%, defoamer 0%~0.2%, thickener 0%~0.5%, and the balance being deionized water.

[0031] Preferably, compound I in the composition is 2%~45%, 5%~40%, 10%~35%, 15%~30%, 20%~25%, etc.; Compound II is present in concentrations of 2%–45%, 5%–40%, 10%–35%, 15%–30%, and 20%–25%, etc. The organic solvents are 1%~90%, 2%~80%, 5%~70%, 10%~60%, 15%~50%, 20%~50%, 30%~40%, etc. Emulsifiers: 1%~8%, 2%~7%, 3-6%, or 4%~5%; Antifreeze 1%~4% or 2%~3%; Defoamer 0.05%~0.1%, or The thickener is 0.1%~0.4% or 0.2%~0.3%.

[0032] According to an embodiment of the present invention, when the composition is prepared into a suspension seed coating agent, it comprises the following components and contents: compound I 0.1%~50%, compound II 0.1%~50%, dispersant 1%~12%, wetting agent 1%~10%, antifreeze agent 1~10%, anticaking agent 0.1~10%, suspending agent 0.1~5%, film-forming agent 1~10%, preservative 0.1~5%, colorant 1~30%, pH adjuster 0.1%~5%, thickener 0.1%~8%, and the balance being deionized water.

[0033] Preferably, compound I in the composition is 2%~45%, 5%~40%, 10%~35%, 15%~30%, 20%~25%, etc.; Compound II is present in concentrations of 2%–45%, 5%–40%, 10%–35%, 15%–30%, and 20%–25%, etc. The dispersant concentration is 2%~10%, 3%~8%, 4%~7%, or 5%~6%; The wetting agent is 1%~8%, 2%~7%, 3-6% or 4%~5%; Antifreeze is 1%~8%, 2%~7%, 3-6%, or 4%~5%; Anti-caking agents are available at concentrations of 0.5%–9%, ​​1%–8%, 2%–7%, 3–6%, or 4%–5%. The suspending agent is 0.2%~4%, 0.5%~3.5%, 1%~3%, 1.5%~2.5%, or 1.8%~2%; The film-forming agent is 2%~9%, 3%~8%, 4%~7%, or 5%~6%; The preservative content is 0.2%~4%, 0.5%~3.5%, 1%~3%, 1.5%~2.5%, or 1.8%~2%; The colorant is 2%~25%, 3%~20%, 5%~15%, 8%~12%, or 9%~10%; pH adjuster: 0.1%~5%, 0.2%~4%, 0.5%~3.5%, 1%~3%, 1.5%~2.5%, or 1.8%~2%; The thickener is 0.5%~7%, 1%~6%, 2%~5%, or 3%~4%.

[0034] According to an embodiment of the present invention, when the composition is formulated into a microcapsule suspension, it comprises the following components and contents: compound I 0.1%~50%, compound II 0.1%~50%, polymeric capsule wall material 1~30%, dispersant 2%~10%, organic solvent 1~50%, emulsifier 1%~7%, pH adjuster 0.1%~5%, defoamer 0.01%~2%, thickener 0.1%~8%, antifreeze 0.1%~8%, and the balance being deionized water.

[0035] Preferably, compound I in the composition is 2%~45%, 5%~40%, 10%~35%, 15%~30%, 20%~25%, etc.; Compound II is present in concentrations of 2%–45%, 5%–40%, 10%–35%, 15%–30%, and 20%–25%, etc. Polymer capsule wall material 2~25%, 5~20%, 6~18%, or 10~15%, etc.; The dispersant concentration is 3%~8%, 4%~7%, or 5%~6%; The organic solvents are 2%~45%, 5%~40%, 10%~35%, 15%~30%; or 20%~25%, etc. The emulsifier is 2%~6% or 4%~5%; pH adjuster: 0.2-4%, 0.5%-3.5%, 1%-3%, 1.5%-2.5%, or 1.8%-2%; The defoamer concentrations are 0.05~1.8%, 0.1%~1.5%, 0.5%~1.3%, and 0.8%~1.0%. The thickener is 0.2%~7%, 0.5%~6%, 1%~5%, 1.5%~4%, or 2%~3%; The antifreeze is 0.5%~7%, 1%~6%, 2%~5%, or 3%~4%.

[0036] According to an embodiment of the present invention, when the composition is prepared into a microcapsule suspension, it comprises the following components and contents: compound I 0.1%~50%, compound II 0.1%~50%, polymeric capsule wall material 1%~12%, dispersant 1%~12%, wetting agent 1%~8%, organic solvent 1~50%, emulsifier 1%~8%, defoamer 0.01%~2%, thickener 0.1%~8%, pH adjuster 0.1%~5%, antifreeze agent 0.1%~8%, and the balance being deionized water.

[0037] According to an embodiment of the present invention, when the composition is prepared into an emulsifiable concentrate, it comprises the following components and contents: compound I 0.1%~50%, compound II 0.1%~50%, emulsifier 0~10%, antifreeze 0%~5%, stabilizer 0%~0.5%, and the balance being an organic solvent; Alternatively, Compound I 0.1%~50%, Compound II 0.1%~50%, Emulsifier 0~12%, Stabilizer 0%~0.5%, with the balance being organic solvent.

[0038] According to an embodiment of the present invention, when the composition is prepared into a microemulsion, it comprises the following components and contents: compound I 0.1%~50%, compound II 0.1%~50%, organic solvent 1~50%, emulsifier 1~30%, antifreeze 1%~10%, stabilizer 0.1%~5%, and the balance being deionized water.

[0039] According to an embodiment of the present invention, when the composition is prepared into a dispersible liquid, it comprises the following components and contents: compound I 0.1%~50%, compound II 0.1%~50%, emulsifier 1~30%, antifreeze 1%~10%, stabilizer 0.1%~5%, and the balance being an organic solvent.

[0040] According to an embodiment of the present invention, when the composition is made into granules, it comprises the following components and contents: compound I 0.1%~10%, compound II 0.1%~10%, dispersant 0%~5%, wetting agent 0%~5%, binder 0%~3%, and the balance being filler.

[0041] According to an embodiment of the present invention, the emulsifier may be selected from at least one of the following: sodium lignosulfonate, agricultural emulsion, phenylphenol polyoxyethylene ether phosphate, tristyrylphenol polyoxyethylene ether phosphate triethanolamine salt, benzyl dimethylphenol polyoxyethylene ether, sorbitan fatty acid ester polyoxyethylene ether, sorbitan anhydride oleate (Span-80), fatty alcohol polyoxyethylene ether, sodium alkyl naphthalene sulfonate, sodium isooctanol succinate sulfonate, nonylphenol polyoxyethylene ether phosphate, and castor oil polyoxyethylene ether phosphate.

[0042] According to an embodiment of the present invention, the dispersant may be selected from at least one of the following: glycerol fatty acid polyoxyethylene ether, polyoxyethylene alkyl aryl ether, sodium lignosulfonate, naphthalene sulfonate formaldehyde condensate, fatty alcohol polyoxyethylene ether sulfate, naphthalene sulfonate formaldehyde condensate, sodium naphthalene sulfonate formaldehyde condensate, nonylphenol polyoxyethylene ether, polyoxyethylene lanolin alcohol, alkylphenol polyoxyethylene ether formaldehyde condensate, fatty alcohol polyoxyethylene ether, fatty alcohol polyoxyethylene ether phosphate, polyoxyethylene sorbitan fatty acid ester, and phosphate ester.

[0043] According to an embodiment of the present invention, the wetting agent may be selected from at least one of the following: trisiloxane polyoxyethylene ether, sodium N-lauroyl glutamate, sodium dodecyl sulfate, sodium lauroyl sarcosinate, sodium methylnaphthalene sulfonate formaldehyde condensate, castor oil polyoxyethylene ether, triphenylethylphenol polyoxyethylene ether, sodium dodecylbenzene sulfonate, sodium alkylnaphthalene sulfonate, sodium isooctanol succinate sulfonate, polyoxyethylene alkyl aryl ether, fatty alcohol polyether glycerol fatty acid polyoxyethylene ether, and fatty alcohol polyoxyethylene ether.

[0044] According to an embodiment of the present invention, the adhesive may be selected from at least one of xanthan gum, starch, urea-formaldehyde resin, gelatin, gum arabic, carboxymethyl cellulose, carboxyethyl cellulose, and polyvinyl alcohol.

[0045] According to an embodiment of the present invention, the disintegrant may be selected from at least one of sodium bicarbonate, ammonium sulfate, sodium sulfate, calcium sulfate, and magnesium chloride.

[0046] According to an embodiment of the present invention, the thickener may be selected from at least one of magnesium aluminum silicate, polyvinyl acetate, xanthan gum, gelatin, gum arabic, and polyvinyl alcohol.

[0047] According to an embodiment of the present invention, the defoamer may be selected from at least one of the following: silicone oil, n-octanol, silicone, butyl phosphate, isobutyl phosphate, etc.

[0048] According to an embodiment of the present invention, the antifreeze agent may be selected from at least one of propylene glycol, ethylene glycol, glycerol, etc.

[0049] According to an embodiment of the present invention, the stabilizer may be selected from at least one of the following: triethanolamine, epichlorohydrin, butyl glycidyl ether, triphenyl phosphite, N-soybean oil-based trimethylenediamine, dialkyl succinate acesulfonate, etc.

[0050] According to an embodiment of the present invention, the filler includes solid filler and liquid filler, wherein the solid filler may be selected from at least one of the following: kaolin, attapulgite, diatomaceous earth, silica, bentonite, montmorillonite, calcium carbonate, and talc. The liquid filler may be selected from at least one of soybean oil, castor oil, and mineral oil.

[0051] According to an embodiment of the present invention, the organic solvent may be selected from at least one of the following: ethyl acetate, acetone, isopropanol, 2,2,2-trifluoroethanol, propylene carbonate, benzene, toluene, xylene, dimethylformamide, dimethyl sulfoxide, dichloromethane, cyclohexane, cyclohexanone, N-methylpyrrolidone, and solvent oil (such as 150# solvent oil).

[0052] The present invention also provides the use of the above composition for the prevention and control of pests in agriculture or non-agriculture.

[0053] According to embodiments of the present invention, the pests include: Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera, mites, and other pests; preferably including aphids (e.g., cotton aphids), brown planthoppers (e.g., rice brown planthoppers), and whiteflies (e.g., tomato whiteflies). According to embodiments of the present invention, the pests are Hemiptera pests.

[0054] As one implementation scheme, the pests are selected from the following: cotton aphids, rice brown planthoppers, and tomato whiteflies.

[0055] The present invention also provides a method for controlling agricultural or non-agricultural pests, comprising applying the above composition to plants infested with pests.

[0056] The compositions of this invention can be provided as finished formulations or as single agents. They are mixed directly before use, then diluted with water to achieve the desired concentration. They can be applied to crops in any manner, such as spraying, root irrigation, or application. In specific applications, they can also be mixed with other agents such as growth regulators, soil conditioners, herbicides, and nematicides.

[0057] The present invention also provides a method for controlling pests (e.g., invertebrate pests), comprising contacting the pest or its environment with a biologically effective amount of the composition of the present invention as described above.

[0058] According to an embodiment of the present invention, the pest is selected from cotton aphids, rice brown planthoppers, or tomato whiteflies.

[0059] The present invention also provides a spray composition comprising the composition and propellant of the present invention as described above.

[0060] The present invention also provides a bait composition comprising the composition of the present invention as described above; the bait composition optionally further comprises one or more food materials; optionally further comprises an insect attractant; and optionally further comprises a wetting agent.

[0061] The beneficial effects of this invention are: The synergistic composition of the present invention has the following advantages: 1) It has a synergistic effect, which can improve the pest control effect; 2) It broadens the insecticidal spectrum, and since pests often occur in the field in a mixed manner, it has a stronger effect on pests; 3) The two active ingredients have different mechanisms of action, and their mixed use can delay the development of pesticide resistance in pests; 4) It reduces the amount of pesticide applied, the number of applications, and the cost of use. Detailed Implementation

[0062] The technical solution of the present invention will be further described in detail below with reference to specific embodiments. It should be understood that the following embodiments are merely illustrative and explanatory of the present invention, and should not be construed as limiting the scope of protection of the present invention. All technologies implemented based on the above content of the present invention are covered within the scope of protection intended by the present invention.

[0063] Unless otherwise stated, the raw materials and reagents used in the following examples are commercially available products or can be prepared by known methods.

[0064] This invention employs a combination of indoor bioassays and field trials to test the insecticidal efficacy of the composition. It should be noted that any modifications, equivalent substitutions, and improvements made within the spirit and principles of this invention should be included within the scope of protection of this invention.

[0065] Compound I used in the following examples can be prepared by referring to the method of Example 1 in patent document CN119101038B.

[0066] Unless otherwise specified, all percentages in the formulations described in the following examples refer to mass percentages.

[0067] I. Formulation Preparation Example Preparation Example 1: Wettable Powder Compound I 0.1~80% Compound II 0.1~75% Sodium dodecylbenzenesulfonate (wetting agent) 0~8% Sodium lignosulfonate (dispersant) 0~10% Talc (filler) 0~5% Silica (filler) 0~5% Attapulgite soil (fill material) replenished to 100%. The above materials are coarsely pulverized in proportion and then mixed evenly in a mixer. After being pulverized by airflow, the finished product is obtained.

[0068] Preparation Example 2: Water-dispersible granules Compound I 0.1~80% Compound II 0.1~75% Sodium alkylnaphthalene sulfonate (wetting agent) 0~8% Sodium lignosulfonate (dispersant) 0~10% Sodium dodecyl sulfate (wetting agent) 0~8% Ammonium sulfate (disintegrant) 0~20% Kaolin (fill material) replenished to 100%. The active ingredients and various additives are mixed evenly according to the formula, and then the powder is obtained by air jet milling. A certain amount of water is added, the mixture is extruded and granulated, and then dried and sieved to obtain the finished product.

[0069] Preparation Example 3: Suspension Compound I 0.1~50% Compound II 0.1~50% Sodium methylnaphthalenesulfonate formaldehyde condensate (wetting agent) 0~8% Xanthan gum (thickener) 0~0.3% Sodium salt of naphthalenesulfonic acid formaldehyde condensate (dispersant) 0~5% Propylene glycol (antifreeze) 0~5% Replenish deionized water to 100%. The active ingredients and various additives are mixed evenly according to the formula, and then the mixture is subjected to high-speed shearing and sand milling to obtain the finished product.

[0070] Preparation Example 4: Emulsifiable Oil Compound I 0.1~50% Compound II 0.1~50% Tristyrene-phenylphenol polyoxyethylene ether phosphate triethanolamine salt (emulsifier) ​​0~7% Fatty alcohol polyvinyl ether (emulsifier) ​​0~3% Propylene glycol (antifreeze) 0~5% Triethanolamine (stabilizer) 0~0.5% Solvent oil (organic solvent) replenished to 100%. The above raw materials are mixed in proportion to dissolve them into a homogeneous oil phase; after passing inspection, they are metered and packaged to obtain the finished product.

[0071] Preparation Example 5: Water-based emulsion Compound I 0.1~50% Compound II 0.1~50% 2,2,2-Trifluoroethanol (organic solvent) 0~35% Propylene carbonate (organic solvent) 0~30% 150# Solvent Oil (Organic Solvent) 0~30% Span-80 (emulsifier) ​​0~10% Ethylene glycol (antifreeze) 0~5% Silicone oil (defoamer) 0~0.2% Polyvinyl alcohol (thickener) 0~0.5% Replenish deionized water to 100%. According to the formula requirements, the above raw materials are added to the mixing tank and mixed evenly by a high-speed shearing machine to make a water-emulsion. After passing the inspection, it is metered and packaged to become the finished product.

[0072] Preparation Example 6: Emulsifiable Oil Compound I 0.1~50% Compound II 0.1~50% Tristyrene-phenylphenol polyoxyethylene ether phosphate triethanolamine salt (emulsifier) ​​0~6% Fatty alcohol polyvinyl ether (emulsifier) ​​0~6% Triethanolamine (stabilizer) 0~0.5% Solvent oil (organic solvent) replenished to 100%. The above raw materials are mixed in proportion to dissolve them into a homogeneous oil phase; after passing inspection, they are metered and packaged to obtain the finished product.

[0073] Preparation Example 7: Granules Compound I 0.1~10% Compound II 0.1~10% Sodium lignosulfonate (dispersant) 0~5% Sodium dodecyl sulfate (wetting agent) 0~5% Polyvinyl alcohol (adhesive) 0~3% Silica (filler) 0~5% Diatomaceous earth (filler) should be replenished to 100%.

[0074] Mix all components thoroughly according to the formula ratio, crush them, moisten them with water, stir them thoroughly again, granulate them using a screw extruder, dry them, and then sieve them to obtain the finished product.

[0075] In the preparation example above, compound II is selected from at least one of flonicamid, pyridinium quinazoline, pymetrozine, pymetrozine, or diproterenol.

[0076] II. Examples of Indoor Toxicity Testing (1) Tested pests: cotton aphids ( Aphis.gossypii Glover, rice brown planthopper ( Nilaparvata mourning Tomato whitefly ( Bemisia tabaci ).

[0077] (2) Measurement method: 2.1 Methods for determining the toxicity of pests 2.1.1 Insect toxicity determination: Using cotton aphids as test material, the toxicity of compound I, compound II and their mixtures was determined by immersion method.

[0078] Referring to the People's Republic of China Agricultural Industry Standard NY / T 1154.6-2006 "Guidelines for Indoor Bioassay Testing of Pesticides," Part 6: Insecticide Activity Test - Immersion Method, the test agents (including Compound I and Compound II) were first prepared into five concentration gradients using suitable solvents (the type of solvent is such as acetone, methanol, N,N-dimethylformamide, and dimethyl sulfoxide, etc., selected according to their solubility in the sample). 50 (The concentration gradient is set according to the ratio or arithmetic progression), and the compounds I and II to be mixed are respectively calculated according to their LC values. 50 The values ​​are set according to a series of proportions based on mass ratio, and the final mass concentration (referring to the total mass of compound I and compound II) is prepared by different ratios.

[0079] After immersing the target insects in the drug solution for 5-10 seconds, excess solution is absorbed with filter paper, and the insects are transferred to normal rearing conditions. Each treatment is repeated 4 times, with 10-20 insects immersed in each replicate. A corresponding organic solvent treatment without the drug is included as a control.

[0080] 2.1.2 Insect toxicity determination: Using rice brown planthopper as the test material, the toxicity of compound I, compound II and their mixtures was determined by rice pesticide immersion method.

[0081] Referring to the People's Republic of China Agricultural Industry Standard NY / T 1154.11-2008 "Guidelines for Indoor Bioassay Testing of Pesticides," Part 11: Rice Impregnation Method for Insecticides, the test agents (including Compound I and Compound II) were first prepared into five concentration gradients using suitable solvents (the type of solvent, such as acetone, methanol, N,N-dimethylformamide, and dimethyl sulfoxide, etc., selected according to their solubility in the sample). 50 (The concentration gradient is set according to the ratio or arithmetic progression), and the compounds I and II to be mixed are respectively calculated according to their LC values. 50 The values ​​are set according to a series of proportions based on mass ratio, and the final mass concentration (referring to the total mass of compound I and compound II) is prepared by different ratios.

[0082] Immerse the rice stems in the prepared solution for 30 seconds (the immersion time can be extended or shortened appropriately depending on the characteristics of the pesticide), then remove and air dry. Wrap the roots with moistened absorbent cotton to retain moisture, cover with plastic wrap, and place in test tubes, with 3 plants per test tube. Each treatment should be replicated at least 4 times, and a blank control should be provided for the treatment without the pesticide (containing all organic solvents and emulsifiers).

[0083] 2.1.3 Insect toxicity determination: Tomato whiteflies were used as test materials, and the toxicity of compound I, compound II and their mixtures was determined by spraying.

[0084] Referring to the People's Republic of China Agricultural Industry Standard NY / T 1154.9-2008 "Guidelines for Indoor Bioassay Testing of Pesticides," Part 9: Spraying Method for Insecticides, the test agents (including Compound I and Compound II) were first prepared into five concentration gradients using suitable solvents (the type of solvent is such as acetone, methanol, N,N-dimethylformamide, and dimethyl sulfoxide, etc., and the selection is based on their solubility in the sample). 50 (The concentration gradient is set according to the ratio or arithmetic progression), and the compounds I and II to be mixed are respectively calculated according to their LC values. 50 The values ​​are set according to a series of proportions based on mass ratio, and the final mass concentration (referring to the total mass of compound I and compound II) is prepared by different ratios.

[0085] The test agent is sprayed evenly onto the corresponding test material, dried, and then inoculated with test insects, with no fewer than 20 insects per replicate. Each treatment is repeated at least 4 times, and a blank control is set up with a treatment that does not contain the agent (including all organic solvents and emulsifiers).

[0086] According to Sun Yunpei's method, the co-toxicity coefficient (CTC) of two drugs with different ratios is calculated. A CTC ≥ 120 indicates a synergistic effect, a CTC ≤ 80 indicates an antagonistic effect, and 80 < CTC < 120 indicates an additive effect. The CTC of the mixture is calculated according to formulas (1), (2), and (3): (1) In the formula: ATI – Actual Measured Toxicity Index of Mixtures; S – LC50 of standard insecticides 50 The unit is milligrams per liter (mg / L); M – LC of the mixture 50 The unit is milligrams per liter (mg / L). (2) In the formula: TTI – Theoretical Co-toxicity Index of Mixtures; TI A—A. Toxicity index of drug A; P A —The percentage content of drug A in the mixture, expressed as a percentage (%). TI B —Toxicity index of drug B; P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0087] (3) In the formula: CTC – Cotoxicity Coefficient; ATI – Actual Toxicity Index of Mixtures; TTI – Theoretical Toxicity Index of Mixtures.

[0088] 2.2 Indoor pesticide activity assay for cotton aphids This experiment used the immersion method to determine the indoor activity of compound I, compound II and their combination against cotton aphids.

[0089] Example 1: To verify the effects of different ratios of Compound I and flonicamid on cotton aphids, indoor toxicity tests were conducted on different ratios of Compound I and flonicamid against cotton aphids. The corresponding concentrations were prepared according to the weight ratios in the table, and indoor insecticidal tests were carried out. The test results are shown in Table 1.

[0090] Table 1. Results of toxicity assays of Compound I and flupyradifurone, alone and in mixtures, against cotton aphids.

[0091] As shown in Table 1, different ratios of compound I and flonicamid have a significant synergistic effect on the activity of cotton aphids.

[0092] Example 2: To verify the effects of different ratios of Compound I and pyridinequinazoline on cotton aphids, indoor toxicity tests were conducted on different ratios of Compound I and flonicamid against cotton aphids. The corresponding concentrations were prepared according to the weight ratios in the table, and indoor insecticidal tests were carried out. The test results are shown in Table 2.

[0093] Table 2. Results of toxicity assays of Compound I and pyridinequinazoline single agents and their mixtures against cotton aphids.

[0094] As shown in Table 2, different ratios of compound I and pyridinequinazoline significantly enhanced the activity of compound I against cotton aphids.

[0095] Example 3: To verify the effects of different ratios of compound I and acetamiprid on cotton aphids, indoor toxicity tests were conducted on different ratios of compound I and acetamiprid against cotton aphids. The corresponding concentrations were prepared according to the weight ratios in the table, and indoor insecticidal tests were carried out. The test results are shown in Table 3.

[0096] Table 3. Results of toxicity assays of Compound I and cypermethrin, alone and in mixtures, against cotton aphids.

[0097] As shown in Table 3, different ratios of compound I and acetamiprid have a significant synergistic effect on the activity of cotton aphids.

[0098] 2.3 Indoor test for pesticide activity against rice brown planthopper This experiment used the rice pesticide immersion method to determine the indoor activity of compound I, compound II and their combination against rice brown planthopper.

[0099] Example 4: To verify the effects of different ratios of compound I and pymetrozine on rice brown planthopper, indoor toxicity tests were conducted on rice brown planthopper using different ratios of compound I and pymetrozine. The corresponding concentrations were prepared according to the weight ratios in the table, and indoor insecticidal tests were carried out. The test results are shown in Table 4.

[0100] Table 4. Results of toxicity assays of Compound I and pymetrozine, alone and in mixtures, against rice brown planthopper.

[0101] As shown in Table 4, different ratios of compound I and pymetrozine have a significant synergistic effect on the activity of rice brown planthopper.

[0102] 2.4 Indoor test for the activity of pesticides against tomato whiteflies This experiment used a spray method to determine the indoor activity of compound I, compound II, and their combinations against tomato whiteflies.

[0103] Example 5: To verify the effects of different ratios of Compound I and Biprofen on tomato whiteflies, indoor toxicity tests were conducted on different ratios of Compound I and Biprofen against tomato whiteflies. The corresponding concentrations were prepared according to the weight ratios in the table, and indoor insecticidal tests were carried out. The test results are shown in Table 5.

[0104] Table 5. Results of toxicity assays of Compound I and bispyribac-methyl, alone and in mixtures, against tomato whiteflies.

[0105] As shown in Table 5, different ratios of compound I and bispyribac-methyl have a significant synergistic effect on the activity of tomato whiteflies.

[0106] III. Field Efficacy Examples To clarify the control efficacy of Compound I and Compound II as single agents and mixtures against cotton aphids, rice brown planthoppers, and tomato whiteflies, multiple efficacy trials were conducted in different regions of China. The formulations used were prepared according to Preparation Examples 1-7, with the formulation in Table 7 as an example (other test formulations were prepared according to the same formulation examples).

[0107] Table 7 Preparation of Compound I with Pymetrozine Suspension at Different Ratios

[0108] Field efficacy test of cotton aphids Experimental method: The pesticide was applied according to the application method specified in the National Standard of the People's Republic of China GB / T 17980.75-2004, when the number of cotton plants infested with aphids reached more than 20%.

[0109] Survey Methods: Following the survey methods specified in the National Standard of the People's Republic of China GB / T 17980.75-2004, after the cotton plant reaches the 4-leaf stage (true leaves), samples were taken at 5 points per plot, with 2 plants fixed at each point. For each plant, 1-2 leaves in the middle section with aphids were fixed, and the number of aphids on the fixed leaves was counted. The initial aphid population was surveyed before pesticide application, and the number of live aphids was surveyed at 1, 3, and 7 days after application, and the control efficacy (effectiveness) was calculated. The formula for calculating the control efficacy is:

[0110]

[0111] Field efficacy test of rice brown planthopper Experimental method: The application method was specified in the National Standard of the People's Republic of China GB / T 17980.5-2004. Application was carried out in the field when the brown planthopper population reached 1000-2000 per 100 rice plants.

[0112] Survey Methods: Following the survey methods specified in the People's Republic of China National Standard GB / T 17980.5-2004, 10-20 points were surveyed per plot using the parallel skip method, with 2 planthoppers per point. The number of planthoppers floating on the water surface between the rice plants was investigated. The initial planthopper population was surveyed before pesticide application, and surveys were conducted again at 1, 3, and 7 days after application, and the control efficacy (effectiveness) was calculated. The formula for calculating the control efficacy is:

[0113]

[0114] Tomato Whitefly Field Efficacy Trial Experimental method: The application method was specified in the National Standard of the People's Republic of China GB / T 17980.16-2004. Application was carried out when there were 2-5 adult insects per plant after crop transplanting.

[0115] Survey Methods: Following the survey methods specified in the People's Republic of China National Standard GB / T 17980.16-2004, 10 crops were marked in each plot. The undersides of the leaves were carefully examined, and the number of surviving adults was recorded. The initial insect population was surveyed before pesticide application, and surveys were conducted again at 1, 3, and 7 days after application. The control efficacy was then calculated. The formula for calculating the control efficacy is:

[0116]

[0117] Table 8. Field efficacy tests of Compound I and Flupyradifurone against cotton aphids.

[0118] Note: Different letters after the data in the same column indicate that the differences are significant at the P<0.05 level according to Duncan's new multiple range test, and the same applies below.

[0119] In the table above, Example 7, 10% Compound I·Flupyridine Suspension (1:4), was prepared according to Preparation Example 3. Here, 10% refers to the total mass percentage of the active substances (Compound I and Flupyridine), and 1:4 refers to the mass ratio of the two active ingredients, Compound I and Flupyridine.

[0120] In Example 8, the 10% Compound I·fluoxetine suspension (1:3) was prepared according to Preparation Example 3. Here, 10% refers to the total mass percentage of the active substances (Compound I and Fluoxetine), and 1:3 refers to the mass ratio of the two active ingredients, Compound I and Fluoxetine.

[0121] The 10% Compound I suspension in Comparative Example 1 was also prepared according to Preparation Example 3, where 10% refers to the mass percentage of the active substance, Compound I. The other examples and comparative examples below were also prepared in this manner, and will not be described in detail here.

[0122] The results in the table show that, when controlling cotton aphids, the combination of compound I and flonicamid, compared with single agents, at the same dosage of active ingredient (comparative examples 1 and 2 corresponding to Examples 7-13), significantly enhanced the 1-day, 3-day, and 7-day control efficacy of compound I and pymetrozine against cotton aphids. The 7-day control efficacy still reached over 91.92%, indicating a longer duration of effectiveness.

[0123] Table 9. Field efficacy test of compound I combined with pymetrozine against rice brown planthopper.

[0124] The test results show that, in controlling corn armyworm, the combination of compound I and pymetrozine, compared with single agent, at the same dosage of active ingredient (comparative examples 3 and 4 corresponding to Examples 14-20), exhibits significantly higher control efficacy against rice brown planthopper at 1, 3, and 7 days than single agent. The 7-day control efficacy still reaches over 91.40%, demonstrating a longer residual effect.

[0125] Table 10. Field efficacy test of compound I combined with diprofen to treat tomato whiteflies.

[0126] The test results show that, in controlling tomato whiteflies, the combination of compound I and bispyribac-methyl, compared with single agent, at the same dosage of active ingredient (comparative examples 5 and 6 corresponding to Examples 21-27), demonstrates significantly higher control efficacy of compound I and bispyribac-methyl at 1, 3, and 7 days compared to single agent. The 7-day control efficacy still reaches over 90.62%, indicating a longer duration of effectiveness.

[0127] The embodiments of the present invention have been described above. However, the present invention is not limited to the above embodiments. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A composition comprising compound I and compound II; The compound I is cyclopyridamole, with the chemical name N-(cyclopropylmethyl)-5-methyl-1-(3-methylbutan-2-yl)-N-(pyridazin-4-yl)-1H-pyrazole-4-carboxamide; Compound II is a string-tuning insecticide.

2. The composition according to claim 1, characterized in that, The composition comprises compound I and compound II; compound I is cyclopyridamole, and compound II is selected from any of the following: flonicamid, pyridazolin, cyclopyridamole, pymetrozine, and diclofenac. Preferably, the mass ratio of compound I to compound II is 80:1 to 1:80, more preferably 50:1 to 1:50, and even more preferably 10:1 to 1:50, 50:1 to 1:10, 10:1 to 1:10, 50:1 to 1:10, or 5:1 to 1:

50. Preferably, the composition is used to control pests such as Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera, and mites; preferably, the composition is used to control aphids (e.g., cotton aphids), brown planthoppers (e.g., rice brown planthoppers), and whiteflies (e.g., tomato whiteflies). Preferably, based on the total weight of the composition as 100%, the sum of the mass of compound I and compound II in the composition is 1-80%, more preferably 2-70%, 5-65%, or 10-60%.

3. The composition according to claim 1 or 2, characterized in that, The composition comprises compound I and compound II; compound I is cyclopyridaben and compound II is flonicamid; preferably, the mass ratio of compound I to compound II is 80:1 to 1:80, more preferably 50:1 to 1:50, and even more preferably 10:1 to 1:50; preferably, the composition is used to control Hemiptera pests; preferably, the composition is used to control aphids (e.g., cotton aphids). Preferably, the composition comprises compound I and compound II; compound I is cyclopyridazine, and compound II is pyridinequinazoline; preferably, the mass ratio of compound I to compound II is 80:1 to 1:80, more preferably 50:1 to 1:50, and even more preferably 50:1 to 1:10; preferably, the composition is used to control Hemiptera pests; preferably, the composition is used to control aphids (e.g., cotton aphids). Preferably, the composition comprises compound I and compound II; compound I is cyclopyridaben and compound II is cyclopyridabenamide; preferably, the mass ratio of compound I to compound II is 80:1 to 1:80, more preferably 50:1 to 1:50, and even more preferably 10:1 to 1:10; preferably, the composition is used to control Hemiptera pests; preferably, the composition is used to control aphids (e.g., cotton aphids). Preferably, the composition comprises compound I and compound II; compound I is cyclopyridaben and compound II is pymetrozine; preferably, the mass ratio of compound I to compound II is 80:1 to 1:80, more preferably 50:1 to 1:50, and even more preferably 50:1 to 1:10; preferably, the composition is used to control Hemiptera pests; preferably, the composition is used to control brown planthoppers (e.g., rice brown planthopper). Preferably, the composition comprises compound I and compound II; compound I is cyclopyridaben and compound II is dipropionate; preferably, the mass ratio of compound I to compound II is 80:1 to 1:80, more preferably 50:1 to 1:50, and even more preferably 5:1 to 1:50; preferably, the composition is used to control Hemiptera pests; preferably, the composition is used to control whiteflies (e.g., tomato whiteflies).

4. The composition according to any one of claims 1-3, characterized in that, The composition further includes at least one of water, organic solvent, emulsifier, dispersant, wetting agent, thickener, defoamer, stabilizer, binder, disintegrant, antifreeze, anticaking agent, suspending agent, film-forming agent, preservative, colorant, polymeric capsule wall material, pH adjuster, or filler.

5. The composition according to any one of claims 1-4, characterized in that, The dosage forms of the composition include wettable powders, water-dispersible granules, suspensions, water-emulsions, suspension seed coatings, microcapsule suspensions, microcapsule suspension-suspension agents, emulsifiable concentrates, microemulsions, dispersible liquids, or granules.

6. Use of the composition according to any one of claims 1-5 for the control of pests in agricultural or non-agricultural fields; Preferably, the pests include: Pests such as Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera, and mites; preferably including aphids (e.g., cotton aphids), brown planthoppers (e.g., rice brown planthoppers), and whiteflies (e.g., tomato whiteflies).

7. A method for controlling pests in agricultural or non-agricultural fields, characterized in that, The method includes applying the composition of any one of claims 1-5 to plants infested with pests; Preferably, the pests include: Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera, mites, and other pests; preferably including aphids (e.g., cotton aphids), brown planthoppers (e.g., rice brown planthoppers), and whiteflies (e.g., tomato whiteflies). Preferably, the plants include cotton, rice, and tomatoes.

8. A method for controlling pests, characterized in that, This includes contacting the pest or its environment with a biologically effective amount of the composition according to any one of claims 1-5; Preferably, the pests include: Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera, mites, etc.; preferably including aphids (e.g., cotton aphids), brown planthoppers (e.g., rice brown planthoppers), and whiteflies (e.g., tomato whiteflies).

9. A spray composition comprising the composition according to any one of claims 1-5 and a propellant.

10. A bait composition comprising the composition according to any one of claims 1-5; Preferably, the bait composition optionally further comprises one or more food materials; Preferably, the bait composition optionally further includes an insect attractant; Preferably, the bait composition may further include a wetting agent.

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