Liquid transfer device, closed liquid transfer device, and PCR detection system

Abstract

This application relates to a liquid transfer device, a closed liquid transfer apparatus, and a PCR detection system. The closed liquid transfer apparatus includes a first liquid storage module, a second liquid storage module, and a central reaction tube. The first liquid storage module is used to store reagents, and the second liquid storage module is used to store reagents. The central reaction tube includes a tube body and a piston that is sealed and movably connected to the tube body. The tube body is provided with a first transfer hole communicating with the tube body. The tube body rotates to drive the first transfer hole to communicate with one of the first liquid storage module and the second liquid storage module respectively. The piston reciprocates to drive the liquid to be directionally transferred between the tube body and the first liquid storage module and the second liquid storage module respectively. In the closed liquid transfer apparatus of this application embodiment, the modules achieve rapid linkage through the central reaction tube, improving the overall process efficiency. The precise docking of the tube body rotation and the directional drive of the piston ensure the accuracy and controllability of liquid transfer, enabling complex biochemical reactions to be executed efficiently.

Description

Technical Field

[0001] This application relates to the field of in vitro diagnostic technology, and in particular to liquid transfer devices, closed liquid transfer devices, and PCR detection systems. Background Technology

[0002] Currently, biochemical experiments mainly rely on robotic arm workstations. These workstations perform closed-loop detection operations within the equipment using robotic arms. When multiple complex biochemical reactions are involved, each step requires precise liquid transfer and strict process control. While traditional robotic arm workstations can perform basic operations, their low efficiency in transferring liquids between different reaction chambers and the large amount of residue result in low reaction efficiency and cumbersome operation.

[0003] Furthermore, in open-loop liquid handling environments, pipette tips need to be changed frequently to avoid cross-contamination between samples. Even so, in the open-loop equipment environment, the flow of different samples and reagents is still inevitably exposed to open spaces, which can easily lead to cross-contamination and seriously affect the purity of the samples.

[0004] In addition, in some applications, the liquid after the reaction needs to be collected for subsequent operations, but based on the current technology, the collection of the liquid after the reaction is very inconvenient. Summary of the Invention

[0005] Based on this, a liquid transfer device, a closed liquid transfer apparatus, and a PCR detection system are provided to solve the problems of low efficiency and cumbersome operation in the existing liquid transfer and complex biochemical reaction processes.

[0006] An embodiment of the first aspect of this application provides a liquid transfer device, comprising:

[0007] The first liquid storage module is used to store reagents;

[0008] The second liquid storage module is used to store reagents;

[0009] A central reaction tube, comprising a tube body and a piston that is sealed and movably connected to the tube body, wherein the tube body is provided with a first transfer hole communicating with the tube body.

[0010] The tube body rotates to drive the first transfer hole to connect with one of the first liquid storage module and the second liquid storage module respectively;

[0011] The piston reciprocates to drive the liquid to be directionally transferred between the tube and the first liquid storage module and the second liquid storage module.

[0012] In one embodiment, the central reaction tube further includes:

[0013] A first rotary valve is fixedly connected to the pipe body, and the pipe body is driven to rotate through the first rotary valve.

[0014] In one embodiment, the first rotary valve is disposed at the bottom of the pipe body for connection to an external first drive component.

[0015] In one embodiment, the first liquid storage module is disposed around the central reaction tube, and the first liquid storage module includes:

[0016] A first liquid storage chamber is arranged around the central reaction tube;

[0017] A plurality of first liquid storage partitions, one end of which is connected to the outer wall of the tube and the other end of which is connected to the inner wall of the first liquid storage cavity; the plurality of first liquid storage partitions divide the first liquid storage cavity into a plurality of first liquid storage unit cavities.

[0018] In one embodiment, the distance between two adjacent first liquid storage partitions is different, resulting in different volumes of the first liquid storage unit cavity.

[0019] In one embodiment, each of the first liquid storage unit cavities has at least one first liquid storage hole at its bottom, the first liquid storage hole being used to connect the first liquid storage unit cavity with the first transfer hole.

[0020] In one embodiment, the tube body rotates about a first rotation axis;

[0021] A plurality of the first liquid storage holes are distributed in a circular shape with the first rotation axis as the center;

[0022] The distance between the first transfer hole and the first rotation axis is equal to the distance between the first liquid storage hole and the first rotation axis.

[0023] In one embodiment, an inclined first liquid receiving port is provided at one end of the first liquid storage hole connected to the first liquid storage unit cavity.

[0024] In one embodiment, the tube body includes:

[0025] The tube section has an outer wall for connecting to the first liquid storage partition and an inner wall for sealing and movably connecting to the piston.

[0026] The flange end is fixed to the outer wall of the bottom end of the pipe section, and the flange end is used to be fixedly connected to the first rotary valve;

[0027] A first sealing cavity is provided between the pipe section and the flange end, and the first sealing cavity is used to place the first sealing ring.

[0028] In one embodiment, the first transfer hole is formed at the end of the flange;

[0029] The first sealing ring is provided with a first connecting hole, which communicates with the first liquid receiving port;

[0030] The first transfer hole is connected to the tube body at one end and extends to the first connecting hole at the other end. The other end of the first transfer hole is connected to the first liquid storage unit cavity through the first connecting hole, the first liquid storage hole, and the first liquid receiving port.

[0031] In one embodiment, the liquid transfer device further includes a second transfer port for communicating with the first transfer port;

[0032] The second liquid storage module includes:

[0033] A liquid storage tube, wherein a second liquid storage chamber is provided inside the liquid storage tube;

[0034] A plurality of second liquid storage partitions are disposed in the second liquid storage cavity and divide the second liquid storage cavity into a plurality of second liquid storage unit cavities;

[0035] The liquid storage tube rotates to drive different second liquid storage unit cavities to communicate with the second transfer hole, so as to connect the first liquid storage module through the second transfer hole and the first transfer hole.

[0036] In one embodiment, the second liquid storage module further includes:

[0037] The second rotary valve is fixedly connected to the liquid storage tube, and the liquid storage tube is driven to rotate through the second rotary valve.

[0038] In one embodiment, the second rotary valve is disposed at the top of the liquid storage tube for connection to an external third drive component.

[0039] In one embodiment, the second rotary valve includes:

[0040] The valve end is used for connection to an external third-party drive component;

[0041] The connecting end is fixedly disposed at the bottom end of the valve end and is used to be fixedly connected to the liquid storage tube; the connecting end is provided with a plurality of liquid inlets and the liquid inlets are used to communicate with the second liquid storage unit cavity.

[0042] In one embodiment, a plurality of the second liquid storage partitions include:

[0043] A central partition is provided at the center of the liquid storage tube in a vertical direction;

[0044] Several arc-shaped partitions, both ends of which are connected to the inner wall of the second liquid storage cavity, together with the second liquid storage cavity to form a second liquid storage unit cavity;

[0045] A plurality of radial partitions, some of which are connected at one end to the outer wall of the central partition and at the other end to the inner wall of the second liquid storage cavity, together with the central partition and the second liquid storage cavity, to form a second liquid storage unit cavity; some of which are connected at one end to the outer wall of the central partition and at the other end to the outer wall of the arc-shaped partition, together with the central partition, the arc-shaped partition and the second liquid storage cavity, to form a second liquid storage unit cavity;

[0046] At least some of the second liquid storage unit cavities have different volumes.

[0047] In one embodiment, at least one second liquid storage hole is provided at the bottom of the second liquid storage unit cavity, and the second liquid storage hole is used to connect the second liquid storage unit cavity and the second transfer hole.

[0048] In one embodiment, an inclined second liquid receiving port is provided at one end of the second liquid storage hole that connects to the second liquid storage unit cavity.

[0049] In one embodiment, a third sealing ring is provided at the bottom of the liquid storage tube, and the third sealing ring is provided with a plurality of second connecting holes, the second connecting holes communicating with the second liquid receiving port and for communicating with the second transfer hole;

[0050] The first transfer hole is connected to the tube body at one end and extends to the second transfer hole at the other end. The other end of the first transfer hole is connected to the second liquid storage unit cavity through the second connecting hole, the second liquid storage hole, and the second liquid receiving port.

[0051] In one embodiment, the liquid transfer device further includes a first extension channel, one end of which is connected to the first transfer hole and the other end of which is connected to the second transfer hole;

[0052] At least a portion of the flow channel cross-section of the first extended channel is circular.

[0053] In one embodiment, the liquid transfer device further includes:

[0054] Temperature control module, used for temperature control of liquids;

[0055] The tube body rotates to drive the first transfer hole to connect with one of the first liquid storage module, the second liquid storage module, and the temperature control module;

[0056] The piston reciprocates to drive the liquid to be directionally transferred between the tube and the first liquid storage module, the second liquid storage module, and the temperature control module.

[0057] In one embodiment, the temperature control module includes:

[0058] A flow guide tube is provided with a flow guide cavity, which is used to communicate with the first transfer hole; the flow guide tube is used to connect to an external PCR device.

[0059] In one embodiment, the flow guide includes:

[0060] The inner tube is provided with a flow channel, one end of which is connected to the first transfer hole and the other end is connected to the flow cavity.

[0061] The outer guide tube has a connection port at its top, which is sealed to the inner guide tube. The outer guide tube contains the guide cavity.

[0062] The bottom end of the outer guide tube is movable. In the movable state, the bottom end of the outer guide tube changes the distance between the bottom wall of the guide cavity and the inner guide tube by displacement, so that the guide channel is separated from or inserted into the liquid surface of the guide cavity.

[0063] In one embodiment, the outer guide tube is slidably connected to the inner guide tube at the connection port, and the outer guide tube slides relative to the inner guide tube to drive the bottom end of the outer guide tube to move.

[0064] In one embodiment, the inner wall of the outer guide tube is provided with a first sealing protrusion, which is used for a sealing sliding connection with the outer wall of the inner guide tube.

[0065] In one embodiment, the outer wall of the inner guide tube is provided with a second sealing protrusion, which is used to make a sealing sliding connection with the inner wall of the outer guide tube.

[0066] The second sealing protrusion is used to abut against the first sealing protrusion when the outer guide tube slides down to its limit position, so as to limit the outer guide tube.

[0067] In one embodiment, the outer guide tube is fixedly connected to the inner guide tube at the connection port, and the outer guide tube is configured as a telescopic tube, which extends and retracts to drive the bottom end of the outer guide tube to move.

[0068] In one embodiment, the outer guide tube is configured as a bellows.

[0069] In one embodiment, the outer guide tube is configured as a rubber tube;

[0070] The top end of the hose is provided with a first connector;

[0071] The bottom end of the tubing is provided with a second connector, which is used to connect to the amplification tank of the external PCR equipment.

[0072] In one embodiment, the guide tube is configured as a flexible tube for close contact with the amplification tank of an external PCR device.

[0073] In one embodiment, the temperature control module further includes:

[0074] A pressure buffer chamber, which is used to communicate with the flow guiding chamber.

[0075] In one embodiment, the guide tube is provided with a buffer channel, one end of which is connected to the guide cavity and the other end is connected to the pressure buffer cavity;

[0076] The end of the guide tube that connects to the pressure buffer chamber is provided with a bending buffer section.

[0077] In one embodiment, the liquid transfer device further includes a second extension channel, one end of which is connected to the first transfer hole and the other end of which is connected to the flow guide channel;

[0078] At least a portion of the flow channel cross-section of the second extended channel is circular.

[0079] In one embodiment, multiple flow guides are provided;

[0080] The second extension channel is provided in multiple ways to connect the flow channel corresponding to the flow guide pipe with the first transfer hole.

[0081] In one embodiment, the liquid transfer device further includes:

[0082] Waste liquid module, used to collect waste liquid;

[0083] The tube rotates to connect the first transfer hole with the waste liquid module, and the piston moves to drive the liquid to be directionally transferred from the tube to the waste liquid module.

[0084] In one embodiment, the waste liquid module includes:

[0085] Waste liquid chamber;

[0086] Waste liquid outlet, which is located at the upper part of the waste liquid chamber and communicates with the waste liquid chamber, is used to connect the waste liquid chamber with the first transfer hole.

[0087] In one embodiment, the waste liquid module further includes:

[0088] A vent is provided at the upper part of the waste liquid chamber and is in communication with the waste liquid chamber;

[0089] A water-resistant and breathable membrane is provided, which covers the vent and is configured to allow gas to pass through while blocking liquid from passing through, so as to balance the gas pressure in the waste liquid chamber.

[0090] In one embodiment, the liquid transfer device further includes a third extension channel, one end of which is connected to the first transfer hole and the other end of which is connected to the waste liquid outlet.

[0091] At least a portion of the flow channel cross-section of the third extended channel is circular.

[0092] In one embodiment, the waste liquid module is located on the side of the central reaction tube away from the temperature control module; and / or;

[0093] The second liquid storage module and the temperature control module are located on the same side of the central reaction tube.

[0094] In one embodiment, the liquid transfer device further includes:

[0095] A magnetic temperature control module is disposed at the bottom of the tube body. The magnetic temperature control module is configured to attract magnetic beads in the liquid and to control the temperature inside the tube body. The magnetic temperature control module is used for connection to external temperature control equipment.

[0096] An embodiment of the second aspect of this application provides a closed liquid transfer device, comprising:

[0097] The liquid transfer device described in any of the above embodiments;

[0098] The enclosure is enclosed, and the first liquid storage module and the central reaction tube are all disposed on the enclosure, and their vertical projections all fall inside the enclosure.

[0099] In one embodiment, the central reaction tube further includes:

[0100] A first rotary valve is fixedly connected to the pipe body, and the pipe body is driven to rotate through the first rotary valve;

[0101] The first rotary valve is located at the bottom of the pipe body and exposed outside the enclosed housing, for connection to an external first driving component.

[0102] In one embodiment, the enclosed housing includes a first housing; the central reaction tube is disposed within the first housing;

[0103] The first liquid storage module includes a first liquid storage chamber, which is disposed between the first housing and the central reaction tube.

[0104] In one embodiment, a first cover is detachably provided on the top of the first housing, and the first cover is sealed and closed on the first housing; the piston is sealed and movably connected to the first cover, and the piston is exposed outside the first cover for connection with an external second driving component;

[0105] The bottom of the first housing is provided with a first opening, which allows the bottom end of the tube to be connected to a first rotary valve exposed in the first housing;

[0106] A first sealing ring is provided between the tube body and the first opening.

[0107] In one embodiment, the enclosed housing includes a second housing; the second housing is provided with a second transfer hole for communicating with the first transfer hole;

[0108] The second liquid storage module is disposed on the second housing, and the second liquid storage module includes:

[0109] A liquid storage tube is rotatably connected to the second housing, and a second liquid storage chamber is provided inside the liquid storage tube;

[0110] A plurality of second liquid storage partitions are disposed in the second liquid storage cavity and divide the second liquid storage cavity into a plurality of second liquid storage unit cavities;

[0111] The liquid storage tube rotates to drive different second liquid storage unit cavities to communicate with the second transfer hole, so as to connect the first liquid storage module through the second transfer hole and the first transfer hole.

[0112] In one embodiment, the second liquid storage module further includes:

[0113] A second rotary valve is fixedly connected to the liquid storage tube, and the liquid storage tube is driven to rotate through the second rotary valve.

[0114] The second rotary valve is located at the top of the liquid storage tube and is exposed outside the second housing for connection to an external third drive component.

[0115] In one embodiment, a second cover is detachably provided on the top of the second housing, and the second cover is sealed and closed onto the second housing;

[0116] The second cover is provided with a second opening, which allows the second rotary valve to be exposed outside the second housing;

[0117] A second sealing ring is provided for the sealing connection between the second cover and the second rotary valve.

[0118] In one embodiment, the enclosed housing has a first extension channel, one end of which is connected to the first transfer hole and the other end of which is connected to the second transfer hole;

[0119] At least a portion of the flow channel cross-section of the first extended channel is circular.

[0120] In one embodiment, the closed-loop liquid transfer device further includes:

[0121] Temperature control module, used for temperature control of liquids;

[0122] The tube body rotates to drive the first transfer hole to connect with one of the first liquid storage module, the second liquid storage module, and the temperature control module;

[0123] The piston reciprocates to drive the liquid to be directionally transferred between the tube and the first liquid storage module, the second liquid storage module, and the temperature control module.

[0124] In one embodiment, a second extension channel is provided on the enclosed housing, one end of the second extension channel is connected to the first transfer hole, and the other end is connected to the temperature control module;

[0125] At least a portion of the flow channel cross-section of the second extended channel is circular.

[0126] In one embodiment, the closed-loop liquid transfer device further includes:

[0127] Waste liquid module, used to collect waste liquid;

[0128] The tube rotates to connect the first transfer hole with the waste liquid module, and the piston moves to drive the liquid to be directionally transferred from the tube to the waste liquid module.

[0129] In one embodiment, the enclosed housing includes a third housing; the waste liquid module is disposed within the third housing.

[0130] In one embodiment, a third extension channel is provided on the enclosed housing, one end of which is connected to the first transfer hole and the other end is connected to the waste liquid module;

[0131] At least a portion of the flow channel cross-section of the third extended channel is circular.

[0132] An embodiment of the third aspect of this application provides a PCR detection system, comprising:

[0133] PCR equipment; and

[0134] The liquid transfer device as described in the above embodiments or the closed liquid transfer apparatus as described in any of the above embodiments includes a temperature control module for interfacing with the RCR device.

[0135] According to the liquid transfer device, closed liquid transfer apparatus, and PCR detection system of this application, the central reaction tube rotates to connect the first transfer port to each module, and combined with the piston-driven directional liquid transfer, independent docking between different modules and the tube body is achieved. Through this setup, each reaction step forms an independent unit, reducing the sharing of common flow channels and avoiding cross-contamination caused by mixing of different reagents or samples during transfer. The rotation of the central reaction tube can quickly switch the connection state of the first transfer port with different modules, achieving precise docking of the target module. The reciprocating movement of the piston can directly drive the directional transfer of liquid between the tube body and each module, eliminating the need for complex robotic arm pipetting structures, reducing transfer steps, and improving efficiency. Liquid mixing is completed within the central reaction tube, eliminating the need for frequent transfers between multiple independent chambers, simplifying the system structure and operation process; the second liquid storage module, as an extension of the first liquid storage module, can centrally store more reagents, reducing the need for frequent replenishment due to reagent shortages, and further optimizing the continuity of the process. The closed liquid transfer apparatus is closed... case Under completely enclosed conditions, liquid transfer is achieved through the connection of a central reaction tube; both liquid transfer and biochemical reactions occur within the closed system. case The process is completed internally, avoiding direct contact between the external environment and samples, thus physically blocking the path of cross-contamination. In summary, the liquid transfer device, closed liquid transfer apparatus, and PCR detection system in this application integrate modules such as the first liquid storage module, the second liquid storage module, and the central reaction tube into a closed system. case Each module achieves rapid linkage through a central reaction tube, avoiding the cumbersome steps of operating multiple components separately in traditional equipment, preventing cross-contamination, and improving the overall process efficiency. The precise docking of the tube rotation and the directional drive of the piston ensure the accuracy and controllability of liquid transfer, enabling the efficient execution of complex biochemical reactions. Attached Figure Description

[0136] Figure 1 This is a schematic diagram of the structure of a liquid transfer device according to an embodiment of this application.

[0137] Figure 2 This is a cross-sectional perspective view of a liquid transfer device according to an embodiment of this application.

[0138] Figure 3 This is a schematic diagram of the structure of the central reaction tube in a liquid transfer device according to an embodiment of this application.

[0139] Figure 4 This is a schematic diagram illustrating the structure of a liquid transfer device according to an embodiment of this application, showing a first liquid storage partition and a second liquid storage partition.

[0140] Figure 5 This is a cross-sectional top view showing the first transfer hole and the second transfer hole in a liquid transfer device according to an embodiment of this application.

[0141] Figure 6 This is a cross-sectional perspective view showing the first transfer hole and the second transfer hole in a liquid transfer device according to an embodiment of this application.

[0142] Figure 7 This is a schematic diagram of the structure of a liquid transfer device, a closed liquid transfer apparatus, and a PCR detection system according to an embodiment of this application.

[0143] Figure 8 This is a partial cross-sectional view showing the second liquid storage module in a liquid transfer device and a closed liquid transfer apparatus according to an embodiment of this application.

[0144] Figure 9 This is a cross-sectional perspective view showing the second liquid storage module in a liquid transfer device and a closed liquid transfer apparatus according to an embodiment of this application.

[0145] Figure 10 This is a cross-sectional perspective view showing the temperature control module in a liquid transfer device and a closed liquid transfer apparatus according to an embodiment of this application.

[0146] Figure 11 This is a cross-sectional view showing the outer guide tube in a liquid transfer device and a closed liquid transfer apparatus according to an embodiment of this application.

[0147] Figure 12 This is a cross-sectional top view showing the first transfer hole and the second transfer hole in a liquid transfer device and a closed liquid transfer apparatus according to an embodiment of this application.

[0148] Figure 13 This is a schematic diagram showing the waste liquid outlet in a liquid transfer device and a closed liquid transfer apparatus according to an embodiment of this application.

[0149] Figure 14 This is a cross-sectional perspective view of a liquid transfer device and a closed liquid transfer apparatus according to an embodiment of this application.

[0150] Figure 15 This is a schematic diagram illustrating the structure of a sealed liquid transfer device and a PCR detection system according to an embodiment of this application.

[0151] Figure label:

[0152] 100. Closed case ;

[0153] 110. First case ;111, First build Body; 112, First opening; 113, First sealing ring; 1131, First connecting hole;

[0154] 120. Second case ; 121, Second transfer hole; 122, Second build Body; 123, second opening; 124, second sealing ring; 125, third sealing ring; 1251, second connecting hole;

[0155] 130. Third case ;

[0156] 140. First extension trench;

[0157] 150. Second extension trench;

[0158] 160. Third extension ditch;

[0159] 170. Sealing film;

[0160] 200. First liquid storage module;

[0161] 210, First liquid storage chamber; 211, First liquid storage unit chamber; 212, First liquid storage hole; 2121, First liquid receiving port;

[0162] 220. First liquid storage partition;

[0163] 300. Second liquid storage module;

[0164] 310. Liquid storage pipe; 311. Second liquid storage unit cavity; 3111. Second liquid storage hole; 3112. Second liquid receiving port;

[0165] 320. Second liquid storage baffle; 321. Central baffle; 322. Arc-shaped baffle; 323. Radial baffle;

[0166] 330. Second rotary valve; 331. Valve end; 332. Connection end; 3321. Liquid inlet;

[0167] 400. Temperature control module;

[0168] 410. Drainage pipe;

[0169] 411. Inner guide tube; 4111. Guide channel; 4112. Second sealing protrusion; 4113. Buffer channel; 4113a. Bending buffer section;

[0170] 412. Outer guide tube; 4121. Guide cavity; 4122. Connecting port; 4123. First sealing protrusion; 4124. First connector; 4125. Second connector; 4125a. Expanded step; 4126. Sliding sealing ring;

[0171] 420. Pressure buffer chamber;

[0172] 500. Central reaction tube;

[0173] 510. Pipe body;

[0174] 511. Pipe section; 512. Flange end; 5121. First transfer hole;

[0175] 520. Piston;

[0176] 530. First rotary valve; 531. Valve control plate;

[0177] 600, Waste Liquid Module;

[0178] 610. Waste liquid chamber;

[0179] 620. Waste liquid outlet;

[0180] 630. Ventilation opening;

[0181] 640. Water-resistant and breathable membrane;

[0182] 700, Magnetic temperature control module;

[0183] 800. PCR equipment; 810. Amplification tank. Detailed Implementation

[0184] To make the above-mentioned objectives, features, and advantages of this application more apparent and understandable, the specific embodiments of this application are described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of this application. However, this application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this application. Therefore, this application is not limited to the specific embodiments disclosed below.

[0185] In the description of this application, it should be understood that if terms such as "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" appear, these terms indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0186] Furthermore, where the terms "first" and "second" appear, these terms are for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined with "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, where the term "multiple" appears, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0187] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0188] In this application, unless otherwise expressly specified and limited, the use of descriptions such as "above" or "below" the second feature indicates that the first and second features are in direct contact or indirect contact via an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. Similarly, "below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0189] It should be noted that if an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. If an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. If so, the terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used in this application are for illustrative purposes only and do not represent the only possible implementation.

[0190] See Figure 1 and Figure 2 At least one embodiment of this application provides a liquid transfer device, including a first liquid storage module 200, a second liquid storage module 300, and a central reaction tube 500. The first liquid storage module 200 and the second liquid storage module 300 are used to store reagents.

[0191] Combination Figure 2 and Figure 3 The central reaction tube 500 includes a tube body 510 and a piston 520 that is sealed and movably connected inside the tube body 510. The tube body 510 is provided with a first transfer hole 5121 that communicates with the inside of the tube body 510. The tube body 510 rotates to drive the first transfer hole 5121 to communicate with one of the first liquid storage module 200 and the second liquid storage module 300 respectively. The piston 520 reciprocates to drive the liquid to be directionally transferred between the tube body 510 and the first liquid storage module 200 and the second liquid storage module 300 respectively.

[0192] The first liquid storage module 200 is used to store reagents and provide raw materials for biochemical reactions. The second liquid storage module 300 serves as an extension module of the first liquid storage module 200, effectively expanding the liquid storage capacity of this system.

[0193] Understandably, in some embodiments, the reagents stored in the first liquid storage module 200 are mainly used for processes such as termination, magnetic bead binding, washing, sample injection, elution, and water replenishment in sample processing. It serves as the reagent carrier area for the sample pretreatment stage, with a function focused on physical processing and basic environmental adjustment. The reagents stored in the second liquid storage module 300 are mainly molecular reaction reagents used for nucleic acid-related biochemical reactions, such as cyclization, enzyme digestion, termination, reverse transcription, and PCR amplification. Its function is focused on the construction and operation of nucleic acid reaction systems. The second liquid storage module 300 is a low-temperature liquid storage module, providing a stable low-temperature environment for the reagents to ensure reagent activity and experimental repeatability.

[0194] According to the liquid transfer device, closed liquid transfer apparatus, and PCR detection system of this application, the central reaction tube 500, through the rotation of the tube body 510, connects the first transfer port 5121 to each module respectively. Combined with the piston 520 driving the directional transfer of liquid, independent docking between different modules and the tube body 510 is achieved. Through this setup, each reaction step forms an independent unit, reducing the sharing of common flow channels and avoiding cross-contamination caused by mixing of different reagents or samples during transfer. The rotation of the tube body 510 of the central reaction tube 500 can quickly switch the connection state of the first transfer port 5121 with different modules, achieving precise docking of the target module. The reciprocating movement of the piston 520 can directly drive the directional transfer of liquid within the tube body 510 between modules, eliminating the need for a complex robotic arm pipetting structure, reducing transfer steps, and improving efficiency. Liquid mixing is completed within the tube body 510 of the central reaction tube 500, eliminating the need for frequent transfers between multiple independent chambers, thus simplifying the system structure and operating procedures. The second liquid storage module 300, as an extension of the first liquid storage module 200, can centrally store more reagents, reducing the need for frequent replenishment due to reagent shortages and further optimizing the continuity of the process. The closed liquid transfer device achieves liquid transfer through the connection of the central reaction tube 500 under the fully enclosed condition of the closed housing 100. Both liquid transfer and biochemical reactions are completed within the closed housing 100, avoiding direct contact between the external environment and samples, and physically blocking the path of cross-contamination. In summary, the liquid transfer device, closed liquid transfer device, and PCR detection system in this application integrate modules such as the first liquid storage module 200, the second liquid storage module 300, and the central reaction tube 500 into the closed housing 100. These modules achieve rapid linkage through the central reaction tube 500, avoiding the cumbersome steps of multi-component dispersed operation in traditional equipment, preventing cross-contamination, and improving the overall process efficiency. The precise alignment of the rotating tube 510 and the directional drive of the piston 520 ensure the accuracy and controllability of liquid transfer, enabling the efficient execution of complex biochemical reactions.

[0195] See Figure 2 In some embodiments, the central reaction tube 500 further includes a first rotary valve 530, which is fixedly connected to the tube body 510. The first rotary valve 530 drives the tube body 510 to rotate. Driven by the first rotary valve 530, the tube body 510 can be directly driven to rotate synchronously, thereby realizing selective communication between the first transfer hole 5121 on the tube body 510 and different components such as the first liquid storage module 200, the second liquid storage module 300, and the temperature control module 400, providing a basis for the directional transfer of liquid between the modules and the tube body 510.

[0196] See Figure 2In some embodiments, a first rotary valve 530 is disposed at the bottom of the pipe body 510 for connection to an external first driving component. The external driving component may be configured as a motor, which drives the first rotary valve 530 to rotate, thereby indirectly driving the pipe body 510 to rotate, thus precisely controlling the object connected to the first transfer hole 5121.

[0197] It is understandable that the first rotary valve 530 can also be manually controlled to rotate, thereby indirectly driving the pipe body 510 to rotate, thus controlling the connected object of the first transfer hole 5121.

[0198] See Figure 2 In some embodiments, a first liquid storage module 200 is arranged around a central reaction tube 500. The first liquid storage module 200 includes a first liquid storage chamber 210 and a plurality of first liquid storage partitions 220. The first liquid storage chamber 210 is arranged around the central reaction tube 500. One end of the first liquid storage partition 220 is connected to the outer wall of the tube body 510, and the other end is connected to the inner wall of the first liquid storage chamber 210. The plurality of first liquid storage partitions 220 divide the first liquid storage chamber 210 into a plurality of first liquid storage unit chambers 211.

[0199] The first liquid storage module 200 is arranged around the central reaction tube 500, reducing the space occupied inside the system and making the overall structure more compact. This helps to reduce the size of the entire liquid transfer device and also facilitates the rapid docking of the central reaction tube 500 with different first liquid storage unit cavities 211 by rotation, improving the efficiency of liquid transfer. The distances between each first liquid storage unit cavity 211 and the central reaction tube 500 are close, facilitating rapid docking through the rotation of the central reaction tube 500. Since the first liquid storage unit cavities 211 are distributed around the central reaction tube 500, the central reaction tube 500 can quickly connect the first transfer hole 5121 with different first liquid storage unit cavities 211 by rotating the tube body 510. Combined with the reciprocating movement of the piston 520, this completes the directional transfer of liquid, simplifying the liquid handling process and reducing operational complexity.

[0200] Through the above configuration, the first liquid storage chamber 210 provides the basic liquid storage space for the first liquid storage module 200, and the first liquid storage partition 220 divides the overall first liquid storage chamber 210 into multiple independent first liquid storage unit chambers 211. These multiple first liquid storage unit chambers 211 can store different types of reagents separately, avoiding mixing of different reagents during storage and providing a foundation for the precise retrieval of reagents in biochemical reactions. The multiple first liquid storage unit chambers 211 increase the system's liquid storage capacity, meeting the needs of complex biochemical reactions for multiple reagents, eliminating the need for frequent external reagent replenishment, and improving the system's continuous operating capability.

[0201] See Figure 2 and Figure 4In some embodiments, the distance between adjacent first liquid storage partitions 220 is different, resulting in different volumes of the first liquid storage unit cavities 211. Specifically, with other parameters such as height being the same, the volume of the first liquid storage unit cavity 211 is determined by the space between adjacent partitions; the difference in distance directly leads to different volumes of different first liquid storage unit cavities 211. Different biochemical reaction steps have different reagent dosage requirements. First liquid storage unit cavities 211 with different volumes can store different volumes of reagents, directly providing the matching amount of reagents for the reaction without additional metering operations, thus meeting the differentiated reagent dosage requirements in complex biochemical processes. Within the limited space of the first liquid storage chamber 210, volume differentiation is achieved by adjusting the spacing between the partitions, avoiding space waste caused by a uniform volume design and making the use of the liquid storage space more efficient.

[0202] See Figure 2 and Figure 4 In some embodiments, each first liquid storage unit cavity 211 has at least one first liquid storage hole 212 at its bottom, which connects the first liquid storage unit cavity 211 to the first transfer hole 5121. Specifically, each first liquid storage unit cavity 211 has a single-hole first liquid storage hole 212 at its bottom. The first liquid storage hole 212 connects the first liquid storage unit cavity 211 to the first transfer hole 5121 in the central reaction tube 500. When the tube body 510 of the central reaction tube 500 rotates to a specific position, the first transfer hole 5121 can precisely align with the first liquid storage hole 212 at the bottom of the target first liquid storage unit cavity 211, thereby achieving communication between the two.

[0203] Through the above configuration, the single-hole design of the first liquid storage hole 212 ensures that each first liquid storage unit cavity 211 is connected to the first transfer hole 5121 through only one channel, avoiding liquid mixing or misconnection that may occur with multiple channels, and ensuring the accuracy of directional transfer of liquid between a specific first liquid storage unit cavity 211 and the tube body 510. The single-hole structure simplifies the flow channel design and reduces the liquid retention space in the channel compared to multiple channels. Combined with the squeezing action of the piston 520, it can more efficiently transfer the liquid in the first liquid storage unit cavity 211 to the tube body 510, reducing the amount of liquid residue. Combined with the compact layout of the first liquid storage module 200 surrounding the central reaction tube 500, the single-hole first liquid storage hole 212 can be quickly aligned with the first transfer hole 5121 of the central reaction tube 500, achieving efficient connection without a complex positioning structure, further improving the convenience of system operation. Each first liquid storage unit cavity 211 is connected to the tube body 510 through a dedicated first liquid storage hole 212, which enhances the independence of each reaction step, reduces the risk of cross-contamination of different reagents during the transfer process, and provides a reliable guarantee for the construction of a text library under fully enclosed conditions.

[0204] See Figure 2 and Figure 3 Referring to some embodiments, the tube body 510 rotates around a first rotation axis; a plurality of first liquid storage holes 212 are distributed in a circumferential shape with the first rotation axis as the center; the distance between the first transfer hole 5121 and the first rotation axis is equal to the distance between the first liquid storage hole 212 and the first rotation axis. Specifically, a plurality of first liquid storage holes 212 are distributed in a circumferential shape at the bottom of the first liquid storage unit cavity 211 with the first rotation axis as the center. This distribution pattern matches the rotation trajectory of the tube body 510, forming a ring-shaped hole layout centered on the axis. The distance between the first transfer hole 5121 and the first rotation axis is exactly equal to the distance between the first liquid storage hole 212 and the axis. This means that when the tube body 510 rotates around the axis, the first transfer hole 5121 can be precisely aligned with any one of the first liquid storage holes 212 on the circumference, achieving a sealed connection between the two.

[0205] With the above configuration, when the tube body 510 rotates, the first transfer hole 5121 can quickly and accurately align with the target first liquid storage hole 212, avoiding communication failure or liquid leakage due to misalignment, and ensuring efficient liquid transfer between the tube body 510 and the first liquid storage unit cavity 211. No complex positioning and calibration mechanism is required; channel switching can be completed simply by rotating the tube body 510, simplifying the control logic of liquid transfer and reducing operational steps. Simultaneously, the stable concentric structure reduces the risk of mechanical wear and improves the long-term reliability of the system. The circumferential distribution ensures that the first liquid storage holes 212 are evenly arranged around the tube body 510. Combined with the design of the first liquid storage module 200 surrounding the central reaction tube 500, this further compresses the internal space of the system, making the overall structure more compact and meeting the miniaturization and integration requirements of gene sequencing equipment.

[0206] See Figure 2 In some embodiments, an inclined first liquid inlet 2121 is provided at one end of the first liquid storage hole 212 connected to the first liquid storage unit cavity 211. Specifically, the connection between the first liquid storage hole 212 and the first liquid storage unit cavity 211 is not vertical or horizontal, but adopts an inclined structure to form a sloped first liquid inlet 2121, which is used to guide the liquid in the first liquid storage unit cavity 211 into the first liquid storage hole 212.

[0207] Through the above design, the inclined first liquid collection port 2121, guided by its slope, allows the liquid in the first liquid storage unit cavity 211 to flow more smoothly to the first liquid storage hole 212, reducing liquid residue at the bottom of the unit cavity and ensuring that the reagents in the unit cavity are transferred to the tube body 510 to the maximum extent, thus improving reagent utilization. The inclined structure makes the liquid flow more stable, avoiding the liquid impact and bubble generation that may be caused by vertical openings. The reduction of bubbles helps to ensure the accuracy of liquid transfer and reduces the interference of bubbles on biochemical reactions. In a fully enclosed environment, the inclined liquid collection port design can improve the liquid collection effect without additional auxiliary structures, simplifying the internal structure of the system, while reducing the risk of cross-contamination caused by liquid residue and ensuring the purity of the gene sequencing library construction.

[0208] See Figure 2 and Figure 3 In some embodiments, the pipe body 510 includes a pipe section 511 and a flange end 512. The outer wall of the pipe section 511 is used to connect with the first liquid storage partition 220, and the inner wall of the pipe section 511 is used to seal and move with the piston 520. The flange end 512 is fixed to the bottom outer wall of the pipe section 511 and is used to fixally connect with the first rotary valve 530. A first sealing cavity is provided between the pipe section 511 and the flange end 512, and the first sealing cavity is used to place the first sealing ring 113.

[0209] Through the above configuration, the first sealing cavity provides a fixed and enclosed installation space for the first sealing ring 113, ensuring that the sealing ring always fits tightly against the outer wall of the pipe 511, preventing displacement of the sealing ring due to rotation or vibration of the pipe body 510. The flange end 512 not only enhances the structural strength of the bottom end of the pipe body 510, but also ensures more stable transmission of driving force through its rigid connection with the first rotary valve 530, reducing shaking or offset during pipe body 510 rotation and improving the accuracy of the alignment between the first transfer hole 5121 and each liquid storage hole. The fixed connection between the flange end 512 and the first rotary valve 530 increases the contact area, allowing external driving force to be transmitted more evenly to the pipe body 510, avoiding component wear caused by excessive local stress, and extending the service life of the pipe body 510 and the rotary valve.

[0210] See Figure 3 and Figure 5 In some embodiments, the first transfer hole 5121 is formed at the flange end 512. The first sealing ring 113 is provided with a first connecting hole 1131, which communicates with the first liquid receiving port 2121; wherein, one end of the first transfer hole 5121 communicates with the inside of the pipe body 510, and the other end extends to the first connecting hole 1131, and the other end of the first transfer hole 5121 communicates with the first liquid storage unit cavity 211 through the first connecting hole 1131, the first liquid storage hole 212, and the first liquid receiving port 2121.

[0211] With the above configuration, the first transfer hole 5121 is precisely aligned with the first connecting hole 1131 of the first sealing ring 113. This achieves dynamic sealing during the rotation of the tube body 510 through the first sealing ring 113, and ensures efficient liquid flow through the alignment of the channels. The first transfer hole 5121 is directly opened at the flange end 512, and forms a short path connection with the first connecting hole 1131, the first liquid storage hole 212, and the first liquid receiving port 2121, reducing the volume of the common flow channel through which the liquid flows. Combined with the inclined design of the first liquid receiving port 2121, this further reduces liquid residue and improves reagent utilization.

[0212] See Figure 2 , Figure 5 and Figure 6 The liquid is transferred between the first liquid storage module 200 and the tube body 510 via the following sequential paths: first liquid storage unit cavity 211, first liquid receiving port 2121, first liquid storage hole 212, first connecting hole 1131 of the first sealing ring 113, first transfer hole 5121, and inside the tube body 510. The transfer is bidirectional and directional, driven by the piston 520.

[0213] In some embodiments, the liquid transfer device further includes a second transfer hole 121, which is used to connect to the first transfer hole 5121. The second liquid storage module 300 includes a liquid storage pipe 310 and a plurality of second liquid storage partitions 320. A second liquid storage chamber is provided in the liquid storage pipe 310. The second liquid storage partitions 320 are disposed in the second liquid storage chamber and divide the second liquid storage chamber into a plurality of second liquid storage unit chambers 311. The liquid storage pipe 310 is rotated to drive different second liquid storage unit chambers 311 to communicate with the second transfer hole 121, so as to connect the first liquid storage module 200 through the second transfer hole 121 and the first transfer hole 5121.

[0214] Specifically, the second transfer hole 121 communicates with the first transfer hole 5121 of the central reaction tube 500, providing a path for the flow of liquid between the first liquid storage module 200 and the second liquid storage module 300. A second liquid storage chamber is formed inside the liquid storage tube 310, serving as the basic space for storing reagents. A second liquid storage partition 320 is disposed within the second liquid storage chamber, with one end connected to the inner wall of the liquid storage tube 310, and the other end dividing the second liquid storage chamber to form several independent second liquid storage unit cavities 311 for classifying and storing different reagents. When the liquid storage tube 310 rotates, it can cause different second liquid storage unit cavities 311 to align and communicate with the second transfer hole 121 in sequence; at this time, through the docking of the second transfer hole 121 and the first transfer hole 5121, the second liquid storage unit cavity 311 can form a complete liquid channel with the tube body 510 of the first liquid storage module 200 or the central reaction tube 500, realizing the cross-module transfer of reagents.

[0215] With the above configuration, the second liquid storage module 300, as an extension structure independent of the first liquid storage module 200, increases the total liquid storage capacity and reagent capacity of the system through the design of multiple second liquid storage unit cavities 311. This can meet the needs of complex biochemical reactions for multiple reagents without frequent external reagent replenishment, thus improving the system's continuous working capability. The docking of the second transfer hole 121 with the first transfer hole 5121 enables the second liquid storage module 300 to form a linkage with the first liquid storage module 200 and the central reaction tube 500. Liquid can flow across modules in a closed environment, maintaining the relative independence of each module while achieving functional complementarity and enhancing the overall applicability of the system. The rotating design of the liquid storage tube 310 allows different second liquid storage unit cavities 311 to quickly switch their connection state with the second transfer hole 121. Combined with the rotation of the central reaction tube 500 and the piston 520 drive, multiple reagents can be used sequentially without disassembling or replacing modules, reducing operational steps, lowering the risk of cross-contamination, and improving liquid transfer efficiency.

[0216] See Figure 7 , Figure 8 and Figure 9In some embodiments, the second liquid storage module 300 further includes a second rotary valve 330, which is fixedly connected to the liquid storage tube 310. The second rotary valve 330 drives the liquid storage tube 310 to rotate. The action of the second rotary valve 330 directly drives the liquid storage tube 310 to rotate synchronously, thereby achieving selective communication between different second liquid storage unit cavities 311 and the second transfer hole 121, providing a basis for the transfer of liquid between the second liquid storage module 300 and other modules. The fixed connection between the second rotary valve 330 and the liquid storage tube 310 ensures efficient transmission of driving force, enabling the liquid storage tube 310 to rotate precisely at a preset angle, thereby achieving accurate docking of specific second liquid storage unit cavities 311 and the second transfer hole 121, ensuring the directionality and accuracy of liquid transfer, and reducing liquid residue or mixing caused by misalignment. By precisely driving the rotation of the liquid storage tube 310, the second liquid storage module 300 can efficiently cooperate with the action of the central reaction tube 500 to achieve coordinated liquid transfer between multiple modules, meeting the needs of complex biochemical reactions for various reagents.

[0217] In some embodiments, the second rotary valve 330 is disposed at the top of the liquid storage tube 310 for connection to an external third driving component. This allows the second rotary valve 330 to be directly connected to the external third driving component, and the external driving force drives the second rotary valve 330 to rotate, thereby driving the liquid storage tube 310 to rotate, achieving precise control over the communication state between the second liquid storage unit cavity 311 and the second transfer hole 121.

[0218] In some embodiments, the second rotary valve 330 includes a valve end 331 and a connecting end 332. The valve end 331 is used for connection to an external third driving component. The connecting end 332 is fixedly disposed at the bottom end of the valve end 331 and is used for fixed connection to the liquid storage tube 310. The connecting end 332 is provided with a plurality of liquid inlets 3321, which are used to communicate with the second liquid storage unit cavity 311. When the external third driving component drives the valve end 331 to rotate, the connecting end 332 rotates synchronously with the valve end 331, thereby driving the liquid storage tube 310 to rotate, so that the liquid inlets 3321 of the connecting end 332 are aligned and connected with different second liquid storage unit cavities 311. At the same time, in conjunction with the rotation of the liquid storage tube 310, the second liquid storage unit cavity 311 is selectively connected to the second transfer hole 121, completing the transfer of liquid between the second liquid storage module 300 and other modules.

[0219] See Figure 8 and Figure 9Through the above configuration, valve end 331 receives external driving force, while connection end 332 simultaneously transmits power and guides liquid. The inlet 3321 of connection end 332 corresponds one-to-one with the second liquid storage unit cavity 311 and rotates synchronously with the rotary valve. This ensures that when the liquid storage tube 310 rotates, a specific second liquid storage unit cavity 311 can precisely connect to the second transfer hole 121 through the inlet 3321, avoiding mixing of different reagents, reducing the risk of cross-contamination, and maintaining the system's closedness and independence. An external third driving component directly controls the rotary valve through valve end 331. Combined with the rigid connection between connection end 332 and liquid storage tube 310, the rotation angle and speed of liquid storage tube 310 can be precisely controlled, thereby enabling rapid switching between the second liquid storage unit cavity 311 and the transfer channel. This meets the need for sequential access to multiple reagents in complex biochemical reactions, reducing operational steps.

[0220] See Figure 4 and Figure 9 In some embodiments, a plurality of second liquid storage baffles 320 include a central baffle 321, a plurality of arc-shaped baffles 322, and a plurality of radial baffles 323. The central baffle 321 is vertically disposed at the center of the liquid storage tube 310. Both ends of the arc-shaped baffles 322 are connected to the inner wall of the second liquid storage cavity, forming a second liquid storage unit cavity 311 with the second liquid storage cavity. One end of a portion of the radial baffles 323 is connected to the outer wall of the central baffle 321, and the other end is connected to the inner wall of the second liquid storage cavity, forming a second liquid storage unit cavity 311 with the central baffle 321 and the second liquid storage cavity. One end of a portion of the radial baffles 323 is connected to the outer wall of the central baffle 321, and the other end is connected to the outer wall of the arc-shaped baffles 322, forming a second liquid storage unit cavity 311 with the central baffle 321, the arc-shaped baffles 322, and the second liquid storage cavity. The volumes of at least a portion of the second liquid storage unit cavities 311 are different.

[0221] Specifically, the curvature of the arc-shaped partition 322 can be set to different degrees, and the length of the radial partition 323 can be set to different degrees. Through the combination of the above-mentioned different types of partitions, at least some of the second liquid storage unit cavities 311 have different volumes. The combined design of the central partition 321, the arc-shaped partition 322, and the radial partition 323 can divide the limited space of the liquid storage tube 310 into multiple independent unit cavities, and the volume difference can be achieved by adjusting the partition size, avoiding the space waste caused by the uniform volume design, and making the space utilization of the liquid storage tube 310 more efficient. The second liquid storage unit cavities 311 with different volumes can store reagents of different volumes respectively, meeting the differentiated needs for the amount of various reagents in complex biochemical reactions such as gene sequencing text library construction. Matching amounts of reagents can be directly used without additional metering operations, improving operational convenience.

[0222] See Figure 8 and Figure 9With the above configuration, the unit cavities are orderly distributed around the central partition 321. Combined with the rotation of the liquid storage tube 310, the second rotary valve 330 can quickly connect different unit cavities to the second transfer hole 121, ensuring precise docking during liquid transfer and reducing the risk of misalignment or leakage due to unreasonable structural layout. The second liquid storage module 300, as an extension of the first liquid storage module 200, further enhances the system's adaptability to complex biochemical processes through its diversified volume unit cavity design. It can hold more types and quantities of reagents, reducing the frequency of external reagent replenishment and ensuring continuous system operation.

[0223] See Figure 5 and Figure 9 In some embodiments, at least one second liquid storage hole 3111 is provided at the bottom of the second liquid storage unit cavity 311. The second liquid storage hole 3111 is used to connect the second liquid storage unit cavity 311 and the second transfer hole 121. Specifically, each second liquid storage unit cavity 311 is provided with a single-hole second liquid storage hole 3111 at its bottom. Specifically, the second liquid storage hole 3111 connects the second liquid storage unit cavity 311 and the second transfer hole 121. When the liquid storage tube 310 is rotated to a specific position under the drive of the second rotary valve 330, the second liquid storage hole 3111 at the bottom of the target second liquid storage unit cavity 311 can be precisely aligned with the second transfer hole 121, thereby realizing the connection between the two and providing a path for the transfer of liquid between the second liquid storage unit cavity 311 and other modules.

[0224] With the above configuration, the single-hole design of the second liquid storage hole 3111 ensures that each second liquid storage unit cavity 311 is connected to the second transfer hole 121 through only one channel. This avoids reagent mixing or misconnection between different unit cavities that may occur with multiple channels, ensuring that the liquid flows only directionally between the target unit cavity and the external transfer path, thus improving the accuracy of liquid transfer. The single-hole structure simplifies the flow channel design, reduces the liquid retention space in the channel, and can more efficiently transfer the reagent in the unit cavity to the external path, reducing liquid residue and improving reagent utilization. In addition, the single-hole design facilitates precise alignment with the second transfer hole 121. When the liquid storage tube 310 rotates, the second liquid storage hole 3111 and the second transfer hole 121 can be quickly and sealed, eliminating the need for a complex positioning structure, simplifying the control logic for rotation switching, and improving the efficiency of switching and using multiple reagents.

[0225] See Figure 9In some embodiments, an inclined second liquid receiving port 3112 is provided at one end of the second liquid storage hole 3111 that connects to the second liquid storage unit cavity 311. That is, the connection between the second liquid storage hole 3111 and the second liquid storage unit cavity 311 adopts an inclined structure, forming a sloped second liquid receiving port 3112, used to guide the liquid in the second liquid storage unit cavity 311 into the second liquid storage hole 3111; and each second liquid storage unit cavity 311 has a single-hole design at its bottom, corresponding to and adapted to the inclined liquid receiving port. The inclined second liquid receiving port 3112, guided by the slope, allows the liquid in the second liquid storage unit cavity 311 to flow more smoothly to the second liquid storage hole 3111, reducing liquid residue at the bottom of the unit cavity, ensuring that the reagent is transferred to the second transfer hole 121 to the maximum extent, and improving reagent utilization.

[0226] See Figure 9 In some embodiments, a third sealing ring 125 is provided at the bottom of the liquid storage tube 310. The third sealing ring 125 is provided with a plurality of second connecting holes 1251. The second connecting holes 1251 communicate with the second liquid receiving port 3112 and are used to communicate with the second transfer hole 121. Among them, one end of the first transfer hole 5121 communicates with the inside of the tube body 510, and the other end extends to the second transfer hole 121. The other end of the first transfer hole 5121 communicates with the second liquid storage unit cavity 311 through the second connecting hole 1251, the second liquid storage hole 3111, and the second liquid receiving port 3112.

[0227] Specifically, the third sealing ring 125 is located at the bottom of the liquid storage tube 310. It achieves dynamic sealing by rotating with the liquid storage tube 310, and precisely connects to the second liquid storage hole 3111 and the second transfer hole 121 through the second connecting hole 1251, ensuring efficient liquid flow while maintaining a seal. The second connecting hole 1251 directly connects to the second liquid receiving port 3112 and the second transfer hole 121. Combined with the single-hole design of the second liquid storage hole 3111 and the inclined liquid receiving port, the liquid flow path is significantly shortened, the volume of the common flow channel is reduced, the amount of liquid residue is reduced, and the reagent utilization rate is improved.

[0228] With the above configuration, the liquid transfer path between the second liquid storage module 300 and the tube body 510 is sequentially: second liquid storage unit cavity 311, second liquid receiving port 3112, second liquid storage hole 3111, second connecting hole 1251 of the third sealing ring 125, second transfer hole 121, first transfer hole 5121, and inside the tube body 510, and bidirectional directional transfer is achieved by the piston 520.

[0229] See Figure 9 In some embodiments, the liquid transfer device further includes a first extension channel 140, one end of which is connected to a first transfer hole 5121 and the other end of which is connected to a second transfer hole 121; at least a portion of the flow channel cross-section of the first extension channel 140 is circular.

[0230] With the above design, the inner wall of the circular cross-section flow channel is smooth and without sharp edges, making it less likely for liquid to stagnate on the wall during flow. Compared to the strip cross-section, which is prone to liquid residue at the corners, the circular cross-section significantly reduces liquid residue and improves reagent utilization, especially suitable for systems requiring low residue. Simultaneously, the circular cross-section makes the resistance distribution during liquid flow more uniform, reducing turbulence and dead zones, and increasing the liquid flow rate between the first transfer hole 5121 and the second transfer hole 121. Combined with the piston 520 drive, this enables more efficient cross-module liquid transfer. The reduced residue from the circular cross-section lowers the risk of cross-contamination between different reagents through the first extended channel 140. Combined with the closed nature of the flow channel, this further ensures the purity of biochemical reactions within the fully enclosed system, meeting the high requirements for contamination control in scenarios such as gene sequencing library construction.

[0231] See Figure 7 and Figure 10 In some embodiments, the liquid transfer device further includes a temperature control module 400 for temperature control of the liquid. The tube 510 rotates to drive a first transfer hole 5121 to communicate with one of the first liquid storage module 200, a second liquid storage module 300, and the temperature control module 400. A piston 520 reciprocates to drive the liquid to be directionally transferred within the tube 510 between the first liquid storage module 200, the second liquid storage module 300, and the temperature control module 400. The temperature control module 400 can precisely control the temperature of the liquid to meet the specific temperature requirements of some biochemical reactions.

[0232] See Figure 7 and Figure 10 In some embodiments, the temperature control module 400 includes a flow guide tube 410 with a flow guide cavity 4121 connected to a first transfer port 5121. The flow guide tube 410 is used to connect to an external PCR (Polymerase Chain Reaction) device. Liquid flows into the flow guide cavity 4121 of the flow guide tube 410 and is precisely temperature-controlled by the external PCR device 800. The flow guide cavity 4121 is connected to the first transfer port 5121 and is used to contain the liquid, ensuring that the liquid remains within the flow guide cavity 4121 and does not flow out. The external PCR device 800 does not directly contact the liquid in the flow guide cavity 4121, but only regulates the temperature of the liquid in the flow guide cavity 4121 by acting on the outer wall of the flow guide tube 410, achieving precise temperature control. While ensuring the temperature control function, the system maintains a completely closed environment, reducing the risk of cross-contamination, making it particularly suitable for scenarios with extremely high purity requirements, such as gene sequencing.

[0233] See Figure 10In some embodiments, the guide tube 410 includes an inner guide tube 411 and an outer guide tube 412. The inner guide tube 411 is provided with a guide channel 4111, one end of which is connected to the first transfer hole 5121, and the other end is connected to the guide cavity 4121. The top of the outer guide tube 412 is provided with a connection port 4122, which is sealed to the inner guide tube 411. The outer guide tube 412 is provided with a guide cavity 4121. The bottom end of the outer guide tube 412 is in a movable state. In the movable state, the bottom end of the outer guide tube 412 changes the distance between the bottom wall of the guide cavity 4121 and the inner guide tube 411 by displacement, so that the guide channel 4111 is disengaged from or inserted into the liquid surface of the guide cavity 4121. The sealed connection between the inner guide tube 411 and the outer guide tube 412 maintains the closed environment of the guide cavity 4121. With the displacement adjustment function, channel switching can be achieved without additional valves, which simplifies the structure while maintaining a certain degree of system sealing and adapts to the pollution prevention requirements of scenarios such as gene sequencing.

[0234] Through the above configuration, the liquid in the guide channel 4111 can flow into the guide cavity 4121. After the liquid has been guided, the bottom end of the outer guide tube 412 is moved downward, increasing the distance between the bottom wall of the guide cavity 4121 and the inner guide tube 411. This causes the guide channel 4111 to detach from the liquid surface of the guide cavity 4121, preventing liquid backflow and ensuring the inner guide tube 411 remains detached from the liquid surface of the guide cavity 4121. This avoids the inner guide tube 411, which is inserted into the liquid surface, affecting the light transmission of the liquid and also solves the problem of light transmission from the guide tube 410, thus facilitating liquid mixing. After the test is completed, the bottom end of the outer guide tube 412 is moved upward, decreasing the distance between the bottom wall of the guide cavity 4121 and the inner guide tube 411. This allows the guide channel 4111 to insert into the liquid surface of the guide cavity 4121, enabling the inner guide tube 411 to transfer the liquid back to the central reaction tube 500 for subsequent reactions.

[0235] With the above settings, it is possible to collect tested samples as needed in some application scenarios for subsequent operations, such as during sequencing or text library construction.

[0236] Specifically, in some embodiments, the height of the light-collecting area is 2mm-3mm, that is, the distance between the bottom wall of the guide cavity 4121 of the inner guide tube 411 and the outer guide tube 412 is 2mm-3mm. The displacement stroke of the outer guide tube 412 is 4mm-5mm.

[0237] See Figure 10Through the above-mentioned settings, the contact state between the flow guiding channel 4111 and the liquid surface can be actively controlled by adjusting the displacement of the bottom end of the outer guide tube 412. When it detaches from the liquid surface, it can physically block the liquid backflow path, solving the backflow contamination problem that is prone to occur in traditional static channels. After the inner guide tube 411 detaches from the liquid surface, it avoids the tube body 510 from obstructing the liquid, reducing interference with lighting. At the same time, it eliminates the obstruction of liquid convection caused by the immersion of the tube body 510, which is conducive to the uniform mixing of the liquid during temperature control and improves reaction efficiency. It also ensures efficient liquid transfer and improves the controllability of the liquid flow direction.

[0238] See Figure 10 In some embodiments, the outer guide tube 412 is slidably connected to the inner guide tube 411 at the connection port 4122, and the outer guide tube 412 slides relative to the inner guide tube 411 to drive the bottom end of the outer guide tube 412 to move.

[0239] In some embodiments, the inner wall of the outer guide tube 412 is provided with a first sealing protrusion 4123, which is used to make a sealing sliding connection with the outer wall of the inner guide tube 411.

[0240] In some embodiments, the outer wall of the inner guide tube 411 is provided with a second sealing protrusion 4112, which is used to make a sealing sliding connection with the inner wall of the outer guide tube 412; wherein, the second sealing protrusion 4112 is used to abut against the first sealing protrusion 4123 when the outer guide tube 412 slides down to the limit position, so as to limit the outer guide tube 412.

[0241] With the above configuration, the first sealing protrusion 4123 and the second sealing protrusion 4112 maintain a seal at the connection point when the outer guide tube 412 slides relative to the inner guide tube 411, preventing liquid leakage from the gap, maintaining the closed environment of the guide cavity 4121, meeting the system's full-closure requirements, and reducing the risk of contamination. Without additional drive, the integrated design of the sliding connection and sealing protrusions achieves the displacement adjustment and sealing functions of the outer guide tube 412, simplifying the overall structure of the guide tube 410, reducing potential failure points, lowering assembly complexity, and improving the long-term reliability of the system.

[0242] Specifically, in some embodiments, a sliding sealing ring 4126 is provided at the connection port 4122 of the outer guide tube 412, and the height of the sliding sealing ring 4126 is mm. The sliding sealing ring 4126 is used to form a seal at the sliding connection between the outer guide tube 412 and the inner guide tube 411 to ensure that the liquid does not leak when the two slide relative to each other.

[0243] The overall height of the outer guide tube 412 is .mm. It is understandable that, while meeting the volume requirements of the internal guide cavity 4121, the length of the guide tube 410 should be as short as possible to reduce residue inside the tube.

[0244] When the outer guide tube 412 slides up to its limit position, the height of the top space reserved between the outer guide tube 412 and the closed shell 100 is mm, which is used to avoid structural interference between the outer guide tube 412 and the closed shell 100 when it slides up, and at the same time to provide a buffer margin for the sliding action.

[0245] Through the above-mentioned design, the compact size and reasonable space reservation of the outer guide tube 412 reduce the vibration and wear of the outer guide tube 412 during sliding. Combined with the buffering effect of the sliding sealing ring 4126, it extends the service life of the sealing structure and the tube body 510 and improves the long-term stability of the system.

[0246] In some embodiments, the outer guide tube 412 is fixedly connected to the inner guide tube 411 at the connection port 4122. The outer guide tube 412 is configured as a telescopic tube, and its extension and retraction cause the bottom end of the outer guide tube 412 to move. The extension and retraction characteristics of the outer guide tube 412 itself achieve length changes, thereby causing the bottom end to move and adjusting the distance between the bottom wall of the guide cavity 4121 and the inner guide tube 411, achieving the effect of the guide channel 4111 detaching from or inserting into the liquid surface of the guide cavity 4121. Specifically, when the outer guide tube 412 extends, its overall length increases, the bottom end moves downward, the distance between the bottom wall of the guide cavity 4121 and the inner guide tube 411 increases, the guide channel 4111 detaches from the liquid surface, blocking backflow and preventing the tube body 510 from affecting lighting and mixing. When the outer guide tube 412 contracts, its overall length shortens, the bottom end moves upward, the distance decreases, the guide channel 4111 inserts into the liquid surface, realizing the inflow or backflow of liquid.

[0247] In some embodiments, the outer guide tube 412 is configured as a bellows. The outer guide tube 412 adopts a bellows structure, with its top connection port 4122 fixedly connected to the inner guide tube 411. The overall length changes through the axial expansion and contraction characteristics of the bellows, thereby driving the bottom end displacement. The integrated wall of the bellows has no sliding gaps, and its fixed connection with the inner guide tube 411 can be combined with a static sealing structure to form a reliable seal, preventing liquid leakage from the connection point. Compared to a sliding connection, this further improves sealing performance and reduces the risk of contamination. The expansion and contraction of the bellows is continuous and controllable; the bottom end displacement distance can be precisely controlled by adjusting the expansion and contraction amount, ensuring the relative position of the guide channel 4111 and the liquid surface remains stable, improving operational accuracy.

[0248] See Figure 7 and Figure 11 In some embodiments, the outer tube 412 is configured as a rubber tube; a first connector 4124 is provided at the top end of the rubber tube; a second connector 4125 is provided at the bottom end of the rubber tube, the second connector 4125 being used to connect to the amplification tank 810 of the external PCR device 800.

[0249] Specifically, the bottom of the second connector 4125 is provided with an amplification step 4125a for connection with the amplification tank 810. The outer guide tube 412 is made of heat-shrink tubing, which is heat-shrinkly wrapped around the first connector 4124 and the second connector 4125. With the above configuration, the tubing structure is simple. During amplification, the amplification step 4125a of the second connector 4125 is directly pressed into the amplification tank 810 of the PCR device 800 to achieve rapid docking; with the lifting and lowering action of the amplification tank 810, the entire tubing can be lifted and lowered, thereby adjusting the distance between the bottom wall of the guide cavity 4121 and the inner guide tube 411, completing the separation or insertion of the guide channel 4111 from the liquid surface.

[0250] In some embodiments, adhesive can be applied to the outer walls of the first connector 4124 and the second connector 4125 respectively to improve their sealing performance with the inner wall of the tubing. Specifically, the adhesive layer's stickiness and filling properties ensure a tight fit between the inner wall of the tubing and the outer wall of the connector, eliminating minute gaps between them and further enhancing the sealing effect at the connection. This adhesive application, combined with the heat-shrink wrapping process of the rubber heat-shrink tubing, forms a double sealing guarantee.

[0251] In some embodiments, the hose includes a rubber hose, a latex hose, and a silicone hose. The rubber hose has good wear resistance and aging resistance; the latex hose has excellent elasticity and flexibility; and the silicone hose has high temperature resistance, low temperature resistance, and good chemical stability, making it adaptable to different working environments and usage requirements. The appropriate type of hose can be selected as the outer guide tube 412 according to the actual application scenario to meet functional requirements such as sealing and expansion.

[0252] In some embodiments, the guide tube 410 is configured as a flexible tube, capable of being flexibly pressed down for close contact with the amplification tank 810 of the external PCR device 800. The flexible tube has the characteristics of being bendable and deformable. When docking with the external PCR device 800, the flexible tube can be flexibly pressed down under pressure, and through its own deformation, it can closely fit the surface of the amplification tank 810 of the PCR device 800, forming a stable contact state and ensuring good contact between the two.

[0253] Through the above-described design, the flexible tube's downward pressure characteristic compensates for assembly errors or surface unevenness between the amplification tank 810 and the guide tube 410. Deformation achieves a complete fit with the amplification tank 810, reducing gaps and improving the sealing of the contact area. This prevents heat loss or the entry of external contaminants during temperature control, ensuring temperature control efficiency and liquid purity. The flexible material buffers the impact during docking, preventing damage to the guide tube 410 or amplification tank 810 caused by rigid contact, extending component lifespan, and reducing the precision requirements of docking operations, facilitating quick and safe equipment connection. When the amplification tank 810 of the external PCR device 800 is raised or lowered, the flexible tube can deform synchronously to maintain a tight contact, ensuring no impact on heat conduction or positional fit between the guide tube 410 and the amplification tank 810, thus ensuring the continuity and stability of the temperature control process.

[0254] See Figure 10 In some embodiments, the temperature control module 400 further includes a pressure buffer chamber 420, which is used to communicate with the flow guide chamber 4121. Specifically, the flow guide pipe 410 is provided with a buffer channel 4113, one end of which is connected to the flow guide chamber 4121, and the other end is connected to the pressure buffer chamber 420, forming a liquid flow path between the flow guide chamber 4121 and the pressure buffer chamber 420. The pressure buffer chamber 420 increases the chamber volume of the flow guide chamber 4121, buffering the pressure generated when the liquid is transferred. The original volume of the flow guide chamber 4121 is μl, and the volume of the liquid entering the flow guide chamber 4121 is μl. The pressure buffer chamber 420 can increase the volume by μl. When the liquid is transferred to the flow guide chamber 4121, the excess space can be accommodated by the μl expansion volume of the pressure buffer chamber 420, avoiding excessive pressure caused by space constraints; conversely, when the liquid flows out, the volume of the pressure buffer chamber 420 can compensate for negative pressure and balance the system pressure.

[0255] See Figure 10 In some embodiments, the guide pipe 410 is provided with a buffer channel 4113, one end of which is connected to the guide cavity 4121 and the other end is connected to the pressure buffer cavity 7. A curved buffer portion 4113a is provided at the end of the guide pipe 410 connected to the pressure buffer cavity 420. Specifically, the curved buffer portion 4113aa is provided at the end of the guide pipe 410 connected to the pressure buffer cavity 420. This portion is curved. When gas flows with the liquid or enters the buffer channel 4113 due to pressure changes, the curved buffer portion 4113aa will prolong the gas flow path and increase the gas flow resistance, thereby reducing the speed and pressure of the gas entering the pressure buffer cavity 420 and achieving a pressure reduction effect.

[0256] See Figure 9 , Figure 10 and Figure 12In some embodiments, the liquid transfer device further includes a second extension channel 150, one end of which is connected to the first transfer hole 5121, and the other end is connected to the guide channel 4111; at least a portion of the flow channel cross-section of the second extension channel 150 is circular. Multiple second extension channels 150 are provided to connect the guide channel 4111 of the corresponding guide pipe 410 to the first transfer hole 5121. The circular cross-section, compared to a strip shape, reduces liquid residue, similar to the effect of the first extension channel 140 described above, and will not be elaborated further here.

[0257] In some embodiments, multiple guide tubes 410 are provided; multiple second extension channels 150 are provided correspondingly for connecting the guide channel 4111 of the corresponding guide tube 410 with the first transfer hole 5121.

[0258] See Figure 7 , Figure 13 and Figure 14 In some embodiments, the liquid transfer device further includes a waste liquid module 600 for collecting waste liquid. The pipe body 510 rotates to connect the first transfer hole 5121 to the waste liquid module 600, and the piston 520 moves to drive the liquid to be directionally transferred from the pipe body 510 to the waste liquid module 600. The waste liquid module 600 effectively expands the waste liquid treatment capacity of the system. When waste liquid needs to be discharged, the piston 520 moves to generate pressure, driving the liquid to be directionally transferred from the pipe body 510 of the central reaction pipe 500 through the first transfer hole 5121 to the waste liquid module 600, completing the waste liquid collection. Through the precise connection achieved by the rotation of the pipe body 510 and the directional transfer driven by the piston 520, contact between the waste liquid and the external environment is avoided, while preventing the waste liquid from flowing back into the pipe body 510 or the storage module, further enhancing the system's anti-pollution capability.

[0259] Specifically, the volume of waste liquid at the bottom of the central reaction tube 500 is only μl-μl. By squeezing the piston 520, the waste liquid can be discharged from the tube body 510 into the waste liquid module 600, which can minimize the retention of waste liquid in the tube body 510, avoid interference of residual liquid with subsequent reactions, and improve the purity of the reaction system.

[0260] See Figure 13In some embodiments, the waste liquid module 600 includes a waste liquid chamber 610 and a waste liquid outlet 620. The waste liquid outlet 620 is located at the upper part of the waste liquid chamber 610 and communicates with the waste liquid chamber 610. The waste liquid outlet 620 is used to connect the waste liquid chamber 610 with the first transfer hole 5121. When the tube body 510 rotates to connect the first transfer hole 5121 with the waste liquid outlet 620, the waste liquid in the tube body 510 is driven by the piston 520 to enter the waste liquid chamber 610 sequentially through the first transfer hole 5121 and the waste liquid outlet 620, completing the collection of waste liquid in a closed environment. When the waste liquid enters the waste liquid chamber 610 through the first transfer hole 5121 and the waste liquid outlet 620, the waste liquid will settle at the bottom of the waste liquid chamber 610 under the action of gravity because the waste liquid outlet 620 is located at the top, forming a liquid level difference. This physically blocks the path of the waste liquid flowing back to the first transfer hole 5121, eliminates the risk of cross-contamination caused by backflow, and ensures the purity of the subsequent reaction system.

[0261] See Figure 14 In some embodiments, the waste liquid module 600 further includes a vent 630 and a water-resistant and breathable membrane 640. The vent 630 is located at the upper part of the waste liquid chamber 610 and communicates with it. The water-resistant and breathable membrane 640 covers the vent 630 and is configured to allow gas to pass through while blocking liquid from passing through, thereby balancing the gas pressure inside the waste liquid chamber 610. When waste liquid enters the waste liquid chamber 610 through the waste liquid port 620, the gas inside the chamber can be discharged through the vent 630 and the water-resistant and breathable membrane 640, balancing the gas pressure inside the chamber. When waste liquid transfer stops or needs to be kept closed, the water-resistant and breathable membrane 640 prevents waste liquid from leaking from the vent 630, maintaining the closed state of the waste liquid chamber 610.

[0262] See Figure 10 In some embodiments, the liquid transfer device further includes a third extension channel 160, one end of which is connected to the first transfer hole 5121, and the other end is connected to the waste liquid port 620; at least a portion of the flow channel cross-section of the third extension channel 160 is circular. Compared with a strip shape, the circular cross-section of the third extension channel 160 reduces liquid residue, and has a similar effect to the first extension channel 140 and the second extension channel 150 described above, which will not be repeated here.

[0263] See Figure 7In some embodiments, the waste liquid module 600 is located on the side of the central reaction tube 500 away from the temperature control module 400; and / or, the second liquid storage module 300 and the temperature control module 400 are located on the same side of the central reaction tube 500. The liquid transfer from the central reaction tube 500 to the temperature control module 400 is separated from the waste liquid transfer path to the waste liquid module 600, reducing flow path intersections and lowering the risk of cross-contamination due to shared paths. The same-side layout of the second liquid storage module 300 and the temperature control module 400 brings them closer together, shortening the flow path of liquid from the storage module through the central reaction tube 500 to the temperature control module 400, reducing liquid residue, and simultaneously reducing transfer time and energy loss, thereby improving reaction efficiency.

[0264] See Figure 2 In some embodiments, the liquid transfer device further includes a magnetic temperature control module 700, which is disposed at the bottom of the tube 510. The magnetic temperature control module 700 is configured to attract magnetic beads in the liquid and to control the temperature inside the tube 510. The magnetic temperature control module 700 is used for connection to an external temperature control device.

[0265] Specifically, the liquid in the first liquid storage module 200 and / or the second liquid storage module 300 contains micron-sized magnetic beads, which can be transferred with the liquid in each reaction channel to the tube 510, allowing the magnetic beads to be quickly and uniformly suspended and mixed with the liquid. The magnetic temperature control module 700 is equipped with a magnet capable of attracting the magnetic beads, providing magnetic attraction. It occupies the bottom space of the central reaction tube 500, attracting liquid to the lower space. By controlling the advancement or pulling of the piston 520, it also facilitates the discharge of residual liquid. The magnetic temperature control module 700 is used for connection to external temperature control equipment. Through external equipment, the central reaction tube 500 can be temperature-controlled to provide the temperature control required during the tank construction process.

[0266] With the above setup, micron-sized magnetic beads, when suspended in liquid, can quickly aggregate or disperse through magnetic attraction, accelerating the mixing of liquid components. This is particularly suitable for the efficient mixing of trace liquid systems, shortening reaction equilibrium time and improving reaction efficiency. Magnetic attraction draws the liquid to the lower part of the tube 510, facilitating directional transfer of the liquid when driven by the piston 520, reducing residue caused by liquid adhering to the cavity wall. Combined with the advancement of the piston 520, trace amounts of residual liquid can be more thoroughly pushed to the waste liquid module 600, improving the actual effect of the low-residue design. The magnetic temperature control module 700 is directly installed at the bottom of the tube 510, closer to the reaction liquid. Heat from external temperature control equipment can be efficiently conducted to the liquid through the module, reducing temperature loss, improving temperature control response speed and uniformity, and meeting the stringent temperature accuracy requirements during the container construction process.

[0267] See Figure 7 and Figure 14At least one embodiment of this application proposes a closed liquid transfer device, which includes the liquid transfer equipment and closed assembly of any of the above embodiments; the first liquid storage module 200, the first liquid storage module 200 and the central reaction tube 500 are all disposed on the closed housing 100, and their vertical projections all fall inside the closed housing 100.

[0268] Specifically, the tube body 510 is rotatably connected to the enclosed housing 100. The enclosed housing 100 serves as the foundation of the entire system, providing installation space for the first liquid storage module 200, the second liquid storage module 300, and the central reaction tube 500, and ensuring the airtightness of the entire system.

[0269] According to the closed liquid transfer device of this application embodiment, under the condition of complete enclosure of the closed housing 100, liquid transfer is achieved through docking of the central reaction tube 500. Both liquid transfer and biochemical reaction are completed within the closed housing 100, avoiding direct contact between the external environment and samples, blocking the path of cross-contamination from a physical level, and ensuring sample purity.

[0270] See Figure 14 In some embodiments, the central reaction tube 500 further includes a first rotary valve 530, which is fixedly connected to the tube body 510 and drives the tube body 510 to rotate. The first rotary valve 530 is located at the bottom of the tube body 510 and exposed outside the enclosed housing 100, for connection to an external first driving component. This arrangement facilitates the connection of the first rotary valve 530 to the external first driving component.

[0271] In some embodiments, the first rotary valve 530 is provided with a valve control plate 531, which presses the first rotary valve 530 onto the enclosed housing 100.

[0272] See Figure 14 In some embodiments, the enclosed housing 100 includes a first housing 110; a central reaction tube 500 is disposed within the first housing 110; and the first liquid storage module 200 includes a first liquid storage chamber 210, which is disposed between the first housing 110 and the central reaction tube 500.

[0273] See Figure 2 In some embodiments, a first cover 111 is detachably provided on the top of the first housing 110, and the first cover 111 is sealed to the first housing 110. The first cover 111 can be opened during the initial reagent filling. The first cover 111, which is sealed to the first housing 110, can ensure a closed environment inside the first housing 110 during system operation, preventing liquid leakage or the entry of external contaminants.

[0274] Through the above-described design, the sealing of the first cover 111 ensures a closed environment within the first housing 110, providing a contamination-free space for liquid transfer and biochemical reactions. The detachable design addresses the reagent filling and other operational needs under a closed structure, avoiding the inconvenience caused by complete sealing. Simultaneously, it further reduces the possibility of cross-contamination, ensuring the purity of the constructed gene sequencing library.

[0275] See Figure 14 The piston 520 is sealed and movablely connected to the first cover 111, and the piston 520 is exposed outside the first cover 111 for connection with an external second driving component. The piston 520 of the central reaction tube 500 is sealed and movablely connected to the first cover 111, that is, the piston 520 can reciprocate on the first cover 111 while the connection between the two remains sealed. In addition, the piston 520 is exposed outside the first cover 111 for connection with an external second driving component. The second driving component can be configured as a drive motor, push rod, etc., which drives the piston 520 to reciprocate within the tube 510 through external driving force, thereby accurately driving the liquid to be directionally transferred between the liquid storage modules and the temperature control module 400 within the tube 510, improving the accuracy and efficiency of liquid transfer.

[0276] With the above configuration, the piston 520 is exposed outside the first cover 111, so that the external second driving component does not need to penetrate into the closed housing 100 to drive the piston 520, which reduces interference with the internal structure of the closed housing 100 and simplifies the overall structure of the system.

[0277] See Figure 14 The bottom of the first housing 110 has a first opening 112, through which the bottom end of the tube 510 connects to the first rotary valve 530 exposed outside the first housing 110. A first sealing ring 113 is provided between the tube 510 and the first opening 112. Specifically, through the first opening 112, although the first rotary valve 530 is located outside the closed housing 100, it can directly drive the tube 510 inside the housing to rotate, realizing the transmission of external driving force to the internal structure. The first sealing ring 113 is provided at the connection between the tube 510 and the first opening 112. The first sealing ring 113 tightly fits the outer wall of the tube 510 and the inner wall of the first opening 112 to form a sealing structure, blocking the direct communication between the inside of the closed housing 100 and the external environment.

[0278] See Figure 14Through the above-mentioned design, the sealing effect of the first sealing ring 113 effectively prevents liquid leakage inside the sealed housing 100, while preventing external contaminants from entering the housing through the first opening 112, maintaining a fully enclosed environment for the system and structurally avoiding the risk of cross-contamination during the construction of the gene sequencing text library. The first opening 112 provides space for the connection between the tube body 510 and the external first rotary valve 530, allowing the first rotary valve 530 to receive driving force outside the housing and drive the tube body 510 to rotate. This ensures both the convenience of external driving and the preservation of the housing's sealing properties, ensuring that the rotation of the tube body 510 can precisely control the connection between the first transfer hole 5121 and each module. While sealing, the first sealing ring 113 also buffers the friction between the tube body 510 and the first opening 112 during rotation, reducing component wear and extending the system's service life. At the same time, the elastic contact of the sealing ring can compensate for assembly errors, ensuring the coaxiality of the tube body 510 during rotation and improving the overall structural stability.

[0279] With the above configuration, the first sealing cavity provides a fixed and enclosed installation space for the first sealing ring 113, ensuring that the sealing ring is always tightly fitted to the outer wall of the pipe 511 and the inner wall of the first housing 110, preventing the sealing ring from shifting due to the rotation or vibration of the pipe 510, thereby continuously blocking the connection between the inside of the enclosed housing 100 and the external environment, preventing liquid leakage and external pollution, and ensuring the complete sealing of the system.

[0280] See Figure 8 and Figure 9 In some embodiments, the enclosed housing 100 includes a second housing 120; the second housing 120 is provided with a second transfer hole 121, which is used to connect to the first transfer hole 5121; a second liquid storage module 300 is disposed on the second housing 120, the second liquid storage module 300 includes a liquid storage pipe 310 and a plurality of second liquid storage partitions 320, the liquid storage pipe 310 is rotatably connected to the second housing 120, and a second liquid storage cavity is provided in the liquid storage pipe 310; the second liquid storage partitions 320 are disposed in the second liquid storage cavity and divide the second liquid storage cavity into a plurality of second liquid storage unit cavities 311; wherein, the liquid storage pipe 310 rotates to drive different second liquid storage unit cavities 311 to communicate with the second transfer hole 121, so as to connect the first liquid storage module 200 through the second transfer hole 121 and the first transfer hole 5121.

[0281] In some embodiments, the second liquid storage module 300 further includes a second rotary valve 330, which is fixedly connected to the liquid storage tube 310 and drives the liquid storage tube 310 to rotate. The second rotary valve 330 is disposed on the top of the liquid storage tube 310 and exposed outside the second housing 120 for connection to an external second driving component.

[0282] It is understandable that the second rotary valve 330 can also be manually controlled to rotate, thereby indirectly driving the liquid storage tube 310 to rotate.

[0283] See Figure 8 and Figure 9 In some embodiments, a second cover 122 is detachably provided on the top of the second housing 120, and the second cover 122 is sealed and closed on the second housing 120; the second cover 122 is provided with a second opening 123, and the second opening 123 allows the second rotary valve 330 to be exposed outside the second housing 120; a second sealing ring 124 is provided between the second cover 122 and the second rotary valve 330 for sealing and movable connection.

[0284] Through the above-described configuration, the sealing of the second cover 122 ensures a closed environment within the second housing 120, providing a contamination-free space for reagent storage and liquid transfer in the second liquid storage module 300. The detachable design of the second housing 120 addresses the operational needs of reagent filling within a closed structure, avoiding inconvenience caused by complete enclosure. The second opening 123 provides a channel for the exposed valve end 331, allowing external drive components to rotate the liquid storage tube 310 without compromising the housing's sealing. Simultaneously, the second sealing ring 124 maintains a continuous seal during the rotation of the valve end 331, neither hindering rotation nor preventing liquid leakage or external contaminant entry through the opening, thus maintaining the system's complete enclosure. The sealing and movable connection of the second sealing ring 124 buffers vibrations during valve end 331 rotation, reducing friction and wear between it and the second cover 122, extending component lifespan. Furthermore, the elastic contact of the sealing ring compensates for assembly errors, ensuring coaxiality during valve end 331 rotation and improving the accuracy of the connection between the liquid storage tube 310 and the transfer hole. The sealed design, combined with the sealing effect of the second cover 122, ensures that the reagents in the second liquid storage module 300 are always in a closed environment, avoiding contamination caused by contact with the outside. At the same time, the stable sealing structure ensures pressure balance during liquid transfer, preventing a decrease in liquid transfer efficiency or an increase in residue due to air leakage, thus ensuring the purity and accuracy of the gene sequencing text library construction.

[0285] See Figure 6 In some embodiments, a first extension channel 140 is provided on the closed housing 100. One end of the first extension channel 140 is connected to a first transfer hole 5121, and the other end is connected to a second transfer hole 121. At least a portion of the flow channel cross-section of the first extension channel 140 is circular.

[0286] See Figure 7In some embodiments, the closed liquid transfer device further includes a temperature control module 400 for temperature control of the liquid; wherein, the tube body 510 rotates to drive the first transfer hole 5121 to communicate with one of the first liquid storage module 200, the second liquid storage module 300, and the temperature control module 400 respectively; the piston 520 reciprocates to drive the liquid to be directionally transferred between the first liquid storage module 200, the second liquid storage module 300, and the temperature control module 400 within the tube body 510 respectively.

[0287] See Figure 9 In some embodiments, a second extension channel 150 is provided on the closed housing 100. One end of the second extension channel 150 is connected to the first transfer hole 5121, and the other end is connected to the temperature control module 400. At least a portion of the flow channel cross-section of the second extension channel 150 is circular.

[0288] See Figure 7 and Figure 14 In some embodiments, the closed liquid transfer device further includes a waste liquid module 600 for collecting waste liquid; wherein, the tube body 510 rotates to drive the first transfer hole 5121 to communicate with the waste liquid module 600, and the piston 520 moves to drive the liquid to be directionally transferred from the tube body 510 to the waste liquid module 600.

[0289] See Figure 14 In some embodiments, the enclosed housing 100 includes a third housing 130; the waste liquid module 600 is disposed within the third housing 130.

[0290] See Figure 10 In some embodiments, a third extension channel 160 is provided on the closed housing 100. One end of the third extension channel 160 is connected to the first transfer hole 5121, and the other end is connected to the waste liquid module 600. At least a portion of the flow channel cross-section of the third extension channel 160 is circular.

[0291] See Figure 15It is understood that, in at least one embodiment of this application, the enclosed shell 100 includes a shell body and a sealing membrane 170. The shell body includes a first shell 110, a second shell 120, and a third shell 130. During the processing of the shell body, various functional structures, including cavities and channels, are pre-fabricated inside or on its surface. Cavities include a waste liquid cavity 610 for collecting waste liquid and a pressure buffer cavity 420 for balancing pressure. Channels include a first extension channel 140, a second extension channel 150, and a third extension channel 160 connecting various modules, used for directional flow of liquids or gases, etc. After the above-mentioned cavities and channels are processed, the sealing membrane 170, such as a polymer film, forms a sealed connection with the shell body at the corresponding cavity openings and channel surfaces. That is, the sealing membrane 170 covers the open parts of the cavities and channels, sealing them into independent enclosed spaces, thereby achieving the enclosure of the enclosed shell 100. In addition, the flexibility of the sealing membrane 170 can adapt to slight thermal expansion and contraction, reducing the risk of seal failure.

[0292] See Figure 7 and Figure 15 At least one embodiment of this application provides a PCR detection system, which includes a PCR device 800 and a liquid transfer device as described in the above embodiments or a closed liquid transfer device as described in any of the above embodiments. The liquid transfer device or closed liquid transfer device includes a temperature control module 400 for interfacing with an RCR device.

[0293] According to the PCR detection system in this embodiment, the temperature control module 400 can be connected to the PCR device 800. Liquid is discharged into the flow channel 4121 of the flow channel 410 of the temperature control module 400, where it is precisely temperature-controlled by the external PCR device 800, enabling polymerase chain reaction (PCR). Through this setup, while ensuring temperature control, a relatively closed system environment is maintained, reducing the risk of cross-contamination, making it particularly suitable for scenarios with extremely high purity requirements, such as gene sequencing.

[0294] According to the PCR detection system in this embodiment, the temperature control module 400 can be connected to the PCR device 800. Liquid is discharged into the flow channel 4121 of the flow channel 410 of the temperature control module 400, where it is precisely temperature-controlled by the external PCR device 800, enabling polymerase chain reaction (PCR). Through this setup, while ensuring temperature control, a relatively closed system environment is maintained, reducing the risk of cross-contamination, making it particularly suitable for scenarios with extremely high purity requirements, such as gene sequencing.

[0295] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0296] The embodiments described above are merely illustrative of several implementation methods of this application, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims

1. A liquid transfer device, characterized in that, include: The first liquid storage module (200) is used to store reagents; The second liquid storage module (300) is used to store reagents; A central reaction tube (500) includes a tube body (510) and a piston (520) that is sealed and movably connected inside the tube body (510). The tube body (510) is provided with a first transfer hole (5121) that communicates with the inside of the tube body (510). The tube body (510) rotates to drive the first transfer hole (5121) to communicate with one of the first liquid storage module (200) and the second liquid storage module (300); The piston (520) reciprocates to drive the liquid to be directionally transferred between the first liquid storage module (200) and the second liquid storage module (300) within the tube (510).

2. The liquid transfer device according to claim 1, characterized in that, The central reaction tube (500) also includes: A first rotary valve (530) is fixedly connected to the pipe body (510), and the pipe body (510) is driven to rotate through the first rotary valve (530).

3. The liquid transfer device according to claim 2, characterized in that, The first rotary valve (530) is located at the bottom of the tube body (510) and is used for connection to an external first driving component.

4. The liquid transfer device according to claim 3, characterized in that, The first liquid storage module (200) is disposed around the central reaction tube (500), and the first liquid storage module (200) includes: A first liquid storage chamber (210) is arranged around the central reaction tube (500); A plurality of first liquid storage partitions (220) are provided, one end of which is connected to the outer wall of the tube body (510) and the other end is connected to the inner wall of the first liquid storage cavity (210); the plurality of first liquid storage partitions (220) divide the first liquid storage cavity (210) into a plurality of first liquid storage unit cavities (211).

5. The liquid transfer device according to claim 4, characterized in that, Among the plurality of first liquid storage partitions (220), the distance between two adjacent first liquid storage partitions (220) is different, resulting in different volumes of the first liquid storage unit cavity (211).

6. The liquid transfer device according to claim 4, characterized in that, Each of the first liquid storage unit cavities (211) has at least one first liquid storage hole (212) at its bottom, and the first liquid storage hole (212) is used to connect the first liquid storage unit cavity (211) with the first transfer hole (5121).

7. The liquid transfer device according to claim 6, characterized in that, The tube body (510) rotates about the first rotation axis; A plurality of the first liquid storage holes (212) are distributed in a circular shape with the first rotation axis as the center; The distance between the first transfer hole (5121) and the first rotation axis is equal to the distance between the first liquid storage hole (212) and the first rotation axis.

8. The liquid transfer device according to claim 6, characterized in that, The first liquid storage hole (212) is provided with an inclined first liquid receiving port (2121) at one end connected to the first liquid storage unit cavity (211).

9. The liquid transfer device according to claim 8, characterized in that, The tube body (510) includes: The outer wall of the tube (511) is used to connect with the first liquid storage partition (220), and the inner wall of the tube (511) is used to seal and move in connection with the piston (520). Flange end (512), the flange end (512) is fixed to the bottom outer wall of the pipe (511), the flange end (512) is used to be fixedly connected to the first rotary valve (530); A first sealing cavity is provided between the pipe section (511) and the flange end (512), and the first sealing cavity is used to place the first sealing ring (113).

10. The liquid transfer device according to claim 9, characterized in that, The first transfer hole (5121) is opened at the flange end (512); The first sealing ring (113) is provided with a first connecting hole (1131), which is connected to the first liquid receiving port (2121); One end of the first transfer hole (5121) is connected to the inside of the tube body (510), and the other end extends to the first connecting hole (1131). The other end of the first transfer hole (5121) is connected to the first liquid storage unit cavity (211) through the first connecting hole (1131), the first liquid storage hole (212), and the first liquid receiving port (2121).

11. The liquid transfer device according to claim 1, characterized in that, The liquid transfer device further includes a second transfer hole (121), which is used to connect to the first transfer hole (5121); The second liquid storage module (300) includes: A liquid storage tube (310) is provided with a second liquid storage chamber inside the liquid storage tube (310); A plurality of second liquid storage partitions (320) are disposed in the second liquid storage cavity and divide the second liquid storage cavity into a plurality of second liquid storage unit cavities (311); The liquid storage tube (310) rotates to drive different second liquid storage unit cavities (311) to communicate with the second transfer hole (121), so as to connect the first liquid storage module (200) through the second transfer hole (121) and the first transfer hole (5121).

12. The liquid transfer device according to claim 11, characterized in that, The second liquid storage module (300) also includes: The second rotary valve (330) is fixedly connected to the liquid storage tube (310) and drives the liquid storage tube (310) to rotate.

13. The liquid transfer device according to claim 12, characterized in that, The second rotary valve (330) is located at the top of the liquid storage tube (310) and is used for connection to an external third drive component.

14. The liquid transfer device according to claim 13, characterized in that, The second rotary valve (330) includes: The valve end (331) is used for connection to an external third drive component; The connecting end (332) is fixedly disposed at the bottom end of the valve end (331) and is used to be fixedly connected to the liquid storage tube (310); the connecting end (332) is provided with a plurality of liquid inlets (3321) and the liquid inlets (3321) are used to communicate with the second liquid storage unit cavity (311).

15. The liquid transfer device according to claim 11, characterized in that, Several of the second liquid storage partitions (320) include: A central partition (321) is arranged vertically at the center of the liquid storage tube (310); Several arc-shaped partitions (322) are provided, both ends of which are connected to the inner wall of the second liquid storage cavity, and together with the second liquid storage cavity, they form a second liquid storage unit cavity (311). A plurality of radial partitions (323), some of which are connected at one end to the outer wall of the central partition (321) and at the other end to the inner wall of the second liquid storage chamber, together with the central partition (321) and the second liquid storage chamber to form a second liquid storage unit cavity (311); some of which are connected at one end to the outer wall of the central partition (321) and at the other end to the outer wall of the arc-shaped partition (322), together with the central partition (321), the arc-shaped partition (322) and the second liquid storage chamber to form a second liquid storage unit cavity (311); At least some of the second liquid storage unit cavities (311) have different volumes.

16. The liquid transfer device according to claim 11, characterized in that, The bottom of the second liquid storage unit cavity (311) is provided with at least a second liquid storage hole (3111), which is used to connect the second liquid storage unit cavity (311) and the second transfer hole (121).

17. The liquid transfer device according to claim 16, characterized in that, The second liquid storage hole (3111) is provided with an inclined second liquid receiving port (3112) at one end connected to the second liquid storage unit cavity (311).

18. The liquid transfer device according to claim 17, characterized in that, The bottom of the liquid storage tube (310) is provided with a third sealing ring (125), and the third sealing ring (125) is provided with a plurality of second connecting holes (1251). The second connecting holes (1251) are connected to the second liquid receiving port (3112) and are used to connect to the second transfer hole (121). One end of the first transfer hole (5121) is connected to the inside of the tube body (510), and the other end extends to the second transfer hole (121). The other end of the first transfer hole (5121) is connected to the second liquid storage unit cavity (311) through the second connecting hole (1251), the second liquid storage hole (3111), and the second liquid receiving port (3112).

19. The liquid transfer device according to claim 18, characterized in that, The liquid transfer device further includes a first extension channel (140), one end of which is connected to the first transfer hole (5121), and the other end is connected to the second transfer hole (121); At least a portion of the flow channel cross-section of the first extended channel (140) is circular.

20. The liquid transfer device according to claim 1, characterized in that, The liquid transfer device also includes: Temperature control module (400), used for temperature control of liquids; The tube body (510) rotates to drive the first transfer hole (5121) to connect with one of the first liquid storage module (200), the second liquid storage module (300), and the temperature control module (400); The piston (520) reciprocates to drive the liquid to be directionally transferred between the first liquid storage module (200), the second liquid storage module (300), and the temperature control module (400) within the tube (510).

21. The liquid transfer device according to claim 20, characterized in that, The temperature control module (400) includes: A flow guide tube (410) is provided with a flow guide cavity (4121), which is used to communicate with the first transfer hole (5121); the flow guide tube (410) is used to connect to an external PCR device (800).

22. The liquid transfer device according to claim 21, characterized in that, The guide tube (410) includes: The inner tube (411) is provided with a flow channel (4111), one end of which is used to connect to the first transfer hole (5121), and the other end is used to connect to the flow cavity (4121); The outer guide tube (412) has a connection port (4122) at its top, which is sealed to the inner guide tube (411). The outer guide tube (412) has a guide cavity (4121) inside it. The bottom end of the outer guide tube (412) is in an active state. In the active state, the bottom end of the outer guide tube (412) changes the distance between the bottom wall of the guide cavity (4121) and the inner guide tube (411) by displacement, so that the guide channel (4111) is separated from or inserted into the liquid surface of the guide cavity (4121).

23. The liquid transfer device according to claim 22, characterized in that, The outer guide tube (412) is slidably connected to the inner guide tube (411) at the connection port (4122). The outer guide tube (412) slides relative to the inner guide tube (411) to drive the bottom end of the outer guide tube (412) to move.

24. The liquid transfer device according to claim 23, characterized in that, The inner wall of the outer guide tube (412) is provided with a first sealing protrusion (4123), which is used to make a sealing sliding connection with the outer wall of the inner guide tube (411).

25. The liquid transfer device according to claim 24, characterized in that, The outer wall of the inner guide tube (411) is provided with a second sealing protrusion (4112), which is used to make a sealing sliding connection with the inner wall of the outer guide tube (412). The second sealing protrusion (4112) is used to abut against the first sealing protrusion (4123) when the outer guide tube (412) slides down to the limit position, so as to limit the outer guide tube (412).

26. The liquid transfer device according to claim 22, characterized in that, The outer guide tube (412) is fixedly connected to the inner guide tube (411) at the connection port (4122). The outer guide tube (412) is configured as a telescopic tube. The outer guide tube (412) extends and retracts to drive the bottom end of the outer guide tube (412) to move.

27. The liquid transfer device according to claim 26, characterized in that, The outer guide tube (412) is configured as a corrugated tube.

28. The liquid transfer device according to claim 26, characterized in that, The outer guide tube (412) is configured as a rubber tube; The top end of the hose is provided with a first connector (4124); The bottom end of the tubing is provided with a second connector (4125), which is used to connect to the amplification tank (810) of the external PCR device (800).

29. The liquid transfer device according to claim 21, characterized in that, The guide tube (410) is configured as a flexible tube for close contact with the amplification tank (810) of the external PCR device (800).

30. The liquid transfer device according to claim 21, characterized in that, The temperature control module (400) also includes: A pressure buffer chamber (420) is provided for communication with the flow guide chamber (4121).

31. The liquid transfer device according to claim 30, characterized in that, The guide tube (410) is provided with a buffer channel (4113), one end of which is connected to the guide cavity (4121) and the other end is connected to the pressure buffer cavity (420); A curved buffer section (4113a) is provided at one end of the flow guide pipe (410) that is connected to the pressure buffer chamber (420).

32. The liquid transfer device according to claim 22, characterized in that, The liquid transfer device further includes a second extension channel (150), one end of which is connected to the first transfer hole (5121), and the other end is connected to the flow guide channel (4111); At least a portion of the flow channel cross-section of the second extended channel (150) is circular.

33. The liquid transfer device according to claim 32, characterized in that, Multiple flow guides (410) are provided; The second extension channel (150) is provided with multiple channels for connecting the guide channel (4111) of the guide pipe (410) to the first transfer hole (5121).

34. The liquid transfer device according to claim 20, characterized in that, The liquid transfer device also includes: Waste liquid module (600) for collecting waste liquid; The tube (510) rotates to drive the first transfer hole (5121) to communicate with the waste liquid module (600), and the piston (520) moves to drive the liquid to be directionally transferred from the tube (510) to the waste liquid module (600).

35. The liquid transfer device according to claim 34, characterized in that, The waste liquid module (600) includes: Waste liquid chamber (610); Waste liquid outlet (620) is provided at the upper part of the waste liquid cavity (610) and communicates with the waste liquid cavity (610). The waste liquid outlet (620) is used to connect the waste liquid cavity (610) with the first transfer hole (5121).

36. The liquid transfer device according to claim 35, characterized in that, The waste liquid module (600) also includes: A vent (630) is provided at the upper part of the waste liquid chamber (610) and communicates with the waste liquid chamber (610); A water-resistant and breathable membrane (640), wherein the water-resistant and breathable membrane (640) is covered with build At the vent (630), the water-resistant and breathable membrane (640) is configured to allow gas to pass through while blocking liquid from passing through, so as to balance the gas pressure in the waste liquid chamber (610).

37. The liquid transfer device according to claim 36, characterized in that, The liquid transfer device further includes a third extension channel (160), one end of which is connected to the first transfer hole (5121), and the other end is connected to the waste liquid port (620); At least a portion of the flow channel cross-section of the third extended channel (160) is circular.

38. The liquid transfer device according to claim 34, characterized in that, The waste liquid module (600) is located on the side of the central reaction tube (500) away from the temperature control module (400); and / or; The second liquid storage module (300) and the temperature control module (400) are located on the same side of the central reaction tube (500).

39. The liquid transfer device according to claim 1, characterized in that, The liquid transfer device also includes: A magnetic temperature control module (700) is disposed at the bottom of the tube body (510). The magnetic temperature control module (700) is configured to attract magnetic beads in the liquid and to control the temperature inside the tube body (510). The magnetic temperature control module (700) is used for connecting to an external temperature control device.

40. A closed liquid transfer device, characterized in that, include: The liquid transfer apparatus according to any one of claims 1-39; The closed housing (100) is provided on the first liquid storage module (200) and the central reaction tube (500), and their vertical projections all fall inside the closed housing (100).

41. The closed liquid transfer device according to claim 40, characterized in that, The central reaction tube (500) also includes: A first rotary valve (530) is fixedly connected to the pipe body (510), and the pipe body (510) is driven to rotate through the first rotary valve (530); The first rotary valve (530) is located at the bottom of the tube body (510) and exposed outside the enclosed housing (100) for connection to an external first drive component.

42. The closed liquid transfer device according to claim 41, characterized in that, The enclosed housing (100) includes a first housing (110); the central reaction tube (500) is disposed within the first housing (110); The first liquid storage module (200) includes a first liquid storage chamber (210), and the first liquid storage chamber (210) is disposed between the first housing (110) and the central reaction tube (500).

43. The closed liquid transfer device according to claim 42, characterized in that, The top of the first housing (110) is detachably provided with a first cover (111), which is sealed and closed on the first housing (110); the piston (520) is sealed and movably connected to the first cover (111), and the piston (520) is exposed outside the first cover (111) for connection with an external second driving component. The bottom of the first housing (110) is provided with a first opening (112), and the first opening (112) is used to connect the bottom end of the tube (510) to the first rotary valve (530) exposed in the first housing (110); A first sealing ring (113) is provided between the tube body (510) and the first opening (112).

44. The closed liquid transfer device according to claim 40, characterized in that, The enclosed housing (100) includes a second housing (120); the second housing (120) is provided with a second transfer hole (121), which is used to communicate with the first transfer hole (5121); The second liquid storage module (300) is disposed on the second housing (120), and the second liquid storage module (300) includes: A liquid storage tube (310) is rotatably connected to the second housing (120), and a second liquid storage chamber is provided inside the liquid storage tube (310); A plurality of second liquid storage partitions (320) are disposed in the second liquid storage cavity and divide the second liquid storage cavity into a plurality of second liquid storage unit cavities (311); The liquid storage tube (310) rotates to drive different second liquid storage unit cavities (311) to communicate with the second transfer hole (121), so as to connect the first liquid storage module (200) through the second transfer hole (121) and the first transfer hole (5121).

45. The closed liquid transfer device according to claim 44, characterized in that, The second liquid storage module (300) also includes: The second rotary valve (330) is fixedly connected to the liquid storage tube (310), and the liquid storage tube (310) is driven to rotate through the second rotary valve (330); The second rotary valve (330) is located on the top of the liquid storage tube (310) and exposed outside the second housing (120) for connection to an external third drive component.

46. ​​The closed liquid transfer device according to claim 45, characterized in that, The top of the second housing (120) is detachably provided with a second cover (122), which seals and covers the second housing (120); The second cover (122) is provided with a second opening (123), the second opening (123) allowing the second rotary valve (330) to be exposed outside the second housing (120); A second sealing ring (124) is provided for a sealing connection between the second cover (122) and the second rotary valve (330).

47. The closed liquid transfer device according to claim 46, characterized in that, The enclosed housing (100) has a first extension channel (140), one end of which is connected to the first transfer hole (5121), and the other end is connected to the second transfer hole (121); At least a portion of the flow channel cross-section of the first extended channel (140) is circular.

48. The closed liquid transfer device according to claim 40, characterized in that, The closed liquid transfer device also includes: Temperature control module (400), used for temperature control of liquids; The tube body (510) rotates to drive the first transfer hole (5121) to connect with one of the first liquid storage module (200), the second liquid storage module (300), and the temperature control module (400); The piston (520) reciprocates to drive the liquid to be directionally transferred between the first liquid storage module (200), the second liquid storage module (300), and the temperature control module (400) within the tube (510).

49. The closed liquid transfer device according to claim 48, characterized in that, The enclosed housing (100) has a second extension channel (150), one end of which is connected to the first transfer hole (5121), and the other end is connected to the temperature control module (400). At least a portion of the flow channel cross-section of the second extended channel (150) is circular.

50. The closed liquid transfer device according to claim 40, characterized in that, The closed liquid transfer device also includes: Waste liquid module (600) for collecting waste liquid; The tube (510) rotates to drive the first transfer hole (5121) to communicate with the waste liquid module (600), and the piston (520) moves to drive the liquid to be directionally transferred from the tube (510) to the waste liquid module (600).

51. The closed liquid transfer device according to claim 50, characterized in that, The enclosed housing (100) includes a third housing (130); the waste liquid module (600) is disposed within the third housing (130).

52. The closed liquid transfer device according to claim 51, characterized in that, The enclosed housing (100) has a third extension channel (160), one end of which is connected to the first transfer hole (5121), and the other end is connected to the waste liquid module (600). At least a portion of the flow channel cross-section of the third extended channel (160) is circular.

53. A PCR detection system, characterized in that, include: PCR equipment (800); as well as The liquid transfer device as described in any one of claims 1-39 or the closed liquid transfer apparatus as described in any one of claims 40-52, wherein the liquid transfer device or the closed liquid transfer apparatus includes a temperature control module (400) for interfacing with the RCR device (800).

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