A rapid extraction method of curcuma longa dna

By treating turmeric samples with Chelex 100 resin, the problem of complex and costly DNA extraction from turmeric in existing technologies has been solved, achieving rapid, simple, and low-cost DNA extraction suitable for PCR and molecular identification.

CN122303220APending Publication Date: 2026-06-30FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
View PDF 0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
Filing Date
2026-05-21
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

Existing technologies make it difficult to extract high-quality DNA from turmeric samples quickly, easily, and at low cost, especially due to the inhibitory effects of polysaccharides, polyphenols, pigments, and other components in turmeric during PCR amplification and molecular identification.

Method used

Turmeric samples were treated with Chelex 100 resin, and impurities were removed by grinding, heating and lysis, and filtration or centrifugation to directly obtain DNA that could be used for PCR amplification and molecular identification.

Benefits of technology

It enables rapid, simple, and low-cost extraction of high-quality DNA from turmeric samples, suitable for batch detection, reduces the inhibitory effects of polysaccharides, polyphenols, pigments, and other components, is safe and non-toxic, and is applicable to PCR, qPCR, DNA barcoding detection, and molecular marker analysis.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN122303220A_ABST
    Figure CN122303220A_ABST
Patent Text Reader

Abstract

This invention discloses a rapid method for extracting DNA from turmeric, belonging to the fields of plant molecular biology and nucleic acid extraction technology. The method includes: taking a turmeric sample, grinding, crushing, or homogenizing it, adding nucleic acid lysis buffer and Chelex 100 resin filler, mixing thoroughly, and then heating to lyse the sample, causing the turmeric tissue cells to release DNA; subsequently, removing tissue debris, resin particles, polysaccharides, pigments, and other insoluble impurities by filtration or centrifugation, and collecting the filtrate or supernatant to obtain the initial extracted turmeric DNA. This method is simple to operate, fast in extraction, and low in cost. It does not require organic reagents such as phenol and chloroform, and the obtained DNA can be directly used for PCR amplification, DNA barcoding detection, molecular marker analysis, and identification of the authenticity of turmeric medicinal materials. It is suitable for rapid detection and batch processing of turmeric samples.
Need to check novelty before this filing date? Find Prior Art