Culture solution mixing device for culturing mesenchymal stem cells
By designing a culture medium mixing device with collision blocks and scraping components, the problem of incomplete discharge of materials after mixing in the existing device is solved, and a more efficient cleaning and mixing effect is achieved.
Patent Information
- Application Number
- CN202423236143.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-27
- Publication Date
- 2025-02-11
- Estimated Expiration
- 2034-12-27
AI Technical Summary
The existing culture medium mixing device is not thoroughly discharged after mixing, resulting in more culture medium remaining inside, and staff need to clean it regularly, affecting the stirring efficiency and mixing quality.
A culture medium mixing device including a collision block and a scraping assembly is designed, and the lifting disc and tripod vibrates through an electric push rod, and the culture medium on the mixing rod and the inner wall of the mixing box is used to clean the mixing rod and the mixing chamber.
It effectively reduces the number of cleaning times for staff, improves mixing efficiency and air discharge rate, and reduces the residual condition of culture medium.
Smart Images
Figure CN222446172U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of mesenchymal stem cell culture, in particular to a culture fluid mixing device for mesenchymal stem cell culture. Background Art
[0002] In the culture process of mesenchymal stem cells, the culture medium mixing device plays a vital role. This device is usually used to ensure the uniform distribution of culture medium components and provide a suitable growth environment to promote cell growth and proliferation.
[0003] When the existing culture medium mixing device is discharged after mixing, a large amount of culture medium will remain inside, resulting in incomplete discharge. In addition, a large amount of culture medium will adhere to the stirring rod on the mixing device, which is not only difficult to discharge, but also requires staff to clean it regularly, thereby increasing the labor of the staff. At the same time, long-term adhesion will also affect the stirring efficiency and the mixing quality of the culture medium. Therefore, we propose a culture medium mixing device for mesenchymal stem cell culture. Utility Model Content
[0004] The utility model aims to provide a culture medium mixing device for culturing mesenchymal stem cells, which provides a collision block, specifically, starts an electric push rod to drive the lifting plate to move downward, so that the collision block moves to the top of the tripod and then stops. At this time, when the tripod rotates, a collision effect will occur after the hemispherical protrusion contacts the collision block. Such reciprocating motion can make the tripod vibrate continuously, so that the culture medium attached to the tripod and the stirring rod can be cleaned, and the number of cleaning times by the staff can be reduced. At the same time, after the attached culture medium is cleaned, the mixing efficiency can be improved, the unloading rate can be improved, and the residual situation can be greatly reduced, which solves the problem that when the existing culture medium mixing device is discharged after mixing, a large amount of culture medium will remain inside, resulting in incomplete discharge and the need for regular cleaning by the staff.
[0005] In order to solve the above technical problems, the utility model is realized by the following technical solutions:
[0006] The utility model discloses a culture medium mixing device for culturing mesenchymal stem cells, comprising a mixing box, a fixing seat being fixedly connected to the top of the mixing box, an electric push rod being fixedly connected to the rear position of the top of the mixing box, a stirring assembly being arranged inside the mixing box, the stirring assembly comprising a tripod, three stirring rods being fixedly connected to the bottom of the tripod, three hemispherical protrusions being fixedly connected to the top of the tripod, a lifting plate being arranged above the tripod, the top of the lifting plate being fixedly connected to the bottom output end of the electric push rod, three fixed blocks being fixedly connected inside the lifting plate, a collision block being slidably connected inside the bottom of the fixed block, the collision block penetrating the fixed block and extending to the outside, a spring 1 being fixedly connected to the top of the collision block, and the top of the spring 1 being fixedly connected to the top of the inner wall of the fixed block.
[0007] Furthermore, a motor is fixedly connected to the top of the fixed seat, a rotating shaft is fixedly connected at the center of the tripod, the top of the rotating shaft passes through the mixing box and extends to the outside, the rotating shaft is rotatably connected to the mixing box, and belt pulleys are fixedly connected to the top of the rotating shaft and the bottom output end of the motor, and the two belt pulleys are connected by belt transmission.
[0008] Furthermore, the lifting plate is in a circular ring shape, the bottom of the collision block is in a hemispherical shape, a stabilizing rod is slidably connected inside the collision block, and the top of the stabilizing rod is fixedly connected to the top of the inner wall of the fixed block.
[0009] Furthermore, a scraping assembly is arranged under the tripod, and the scraping assembly includes a scraping rod, the outer side of the scraping rod contacts the inner wall of the mixing box, the bottom of the rotating shaft is fixedly connected to a connecting seat, a movable rod is slidably connected inside the connecting seat, the movable rod passes through the connecting seat and extends to the outside, the side of the movable rod close to the scraping rod is fixedly connected to the scraping rod surface, the outer surface of the movable rod is fixedly connected to a convex ring, the convex ring is arranged inside the connecting seat, a spring 2 is sleeved on the outer side of the movable rod, and the spring 2 is arranged inside the connecting seat.
[0010] Furthermore, a plurality of leakage holes are provided at the bottom of the connection seat, a side of the second spring close to the convex ring is fixedly connected to the surface of the convex ring, and a side of the second spring away from the convex ring is fixedly connected to the inner wall of the connection seat.
[0011] Furthermore, a discharge pipe is fixedly connected to the bottom of the mixing box, a valve is arranged on the outside of the discharge pipe, and a feed hopper is fixedly connected to the left side of the top of the mixing box.
[0012] The utility model has the following beneficial effects:
[0013] The utility model sets a collision block, specifically, starts the electric push rod to drive the lifting plate to move downward, so that the collision block moves to the top of the tripod and then stops. At this time, when the tripod rotates, the hemispherical protrusion contacts the collision block and there will be a collision effect. Such reciprocating can make the tripod vibrate continuously, so that the culture solution attached to the tripod and the stirring rod can be cleaned, and the cleaning times of the staff can be reduced. At the same time, after the attached culture solution is cleaned, the mixing efficiency can be improved, the unloading rate is also improved, and the residual situation is greatly reduced.
[0014] The utility model arranges a scraping component, specifically a rotating shaft that drives the connecting seat to rotate together, and the movable rod drives the scraping rod to rotate together. The scraping rod can be tightly attached to the inner wall of the mixing box under the elastic action of the spring. This method can clean the material attached to the inner wall of the mixing box, improve the mixing effect, make the culture solution mixed more fully, and at the same time can help discharge the material.
[0015] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for describing the embodiments are briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.
[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model;
[0018] Figure 2 This is a schematic diagram of the internal cross-sectional structure of the mixing box of the utility model;
[0019] Figure 3 For this utility model Figure 2 A is a schematic diagram of the enlarged structure of the middle part;
[0020] Figure 4 This is a schematic diagram of the overall structure of the tripod of the utility model;
[0021] Figure 5 It is a schematic diagram of the internal cross-sectional structure of the connecting seat of the utility model.
[0022] In the accompanying drawings, the components represented by the reference numerals are listed as follows:
[0023] 1. Mixing box; 11. Fixed seat; 111. Motor; 12. Electric push rod; 13. Feed hopper; 14. Stirring assembly; 141. Tripod; 411. Hemispherical convex block; 142. Stirring rod; 143. Rotating shaft; 431. Belt pulley; 15. Lifting plate; 151. Fixed block; 152. Collision block; 153. Stabilizing rod; 154. Spring 1; 16. Scraping assembly; 161. Scraping rod; 162. Connecting seat; 163. Movable rod; 164. Convex ring; 165. Spring 2; 166. Leakage hole; 2. Discharge pipe; 21. Valve. DETAILED DESCRIPTION
[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0025] See also Figure 1-Figure 5As shown, the utility model is a culture medium mixing device for culturing mesenchymal stem cells, comprising a mixing box 1, a fixing seat 11 is fixedly connected to the top of the mixing box 1, an electric push rod 12 is fixedly connected to the rear position of the top of the mixing box 1, a stirring assembly 14 is arranged inside the mixing box 1, the stirring assembly 14 comprises a tripod 141, three stirring rods 142 are fixedly connected to the bottom of the tripod 141, three hemispherical protrusions 411 are fixedly connected to the top of the tripod 141, a lifting plate 15 is arranged above the tripod 141, the top of the lifting plate 15 is fixedly connected to the bottom output end of the electric push rod 12, three fixed blocks 151 are fixedly connected inside the lifting plate 15, a collision block 152 is slidably connected to the bottom of the fixed block 151, and the collision block 152 penetrates the fixed The block 151 extends to the outside, and the top of the collision block 152 is fixedly connected with a spring 154, and the top of the spring 154 is fixedly connected to the top of the inner wall of the fixed block 151; by setting the collision block 152, specifically starting the electric push rod 12 to drive the lifting plate 15 to move downward, so that the collision block 152 moves to the top of the tripod 141 and then stops. At this time, when the tripod 141 rotates, the hemispherical protrusion 411 contacts the collision block 152 and a collision effect occurs. Such reciprocating movement can make the tripod 141 vibrate continuously, thereby cleaning the culture solution attached to the tripod 141 and the stirring rod 142, reducing the number of cleaning times for the staff, and at the same time, after cleaning the attached culture solution, the mixing efficiency can be improved, the unloading rate is also improved, and the residual situation is greatly reduced.
[0026] A motor 111 is fixedly connected to the top of the fixed base 11, and a rotating shaft 143 is fixedly connected to the center of the tripod 141. The top of the rotating shaft 143 passes through the mixing box 1 and extends to the outside. The rotating shaft 143 is rotatably connected to the mixing box 1, and a belt pulley 431 is fixedly connected to the top of the rotating shaft 143 and the bottom output end of the motor 111, and the two belt pulleys 431 are connected by belt transmission; when the motor 111 is started to drive the right belt pulley 431 to rotate, the right belt pulley 431 drives the left belt pulley 431 to rotate through the belt, and the left belt pulley 431 drives the rotating shaft 143 to rotate together, and the tripod 141 rotates together, and the stirring rod 142 mixes the materials.
[0027] The lifting plate 15 is in a circular ring shape, the bottom of the collision block 152 is in a hemispherical shape, a stabilizing rod 153 is slidably connected inside the collision block 152, and the top of the stabilizing rod 153 is fixedly connected to the top of the inner wall of the fixed block 151; the stabilizing rod 153 is used to improve the stability of the collision block 152, so that the collision block 152 can remain stable after a collision.
[0028] A scraping assembly 16 is arranged below the tripod 141, and the scraping assembly 16 includes a scraping rod 161, the outer side of the scraping rod 161 contacts the inner wall of the mixing box 1, a connecting seat 162 is fixedly connected to the bottom of the rotating shaft 143, and a movable rod 163 is slidably connected inside the connecting seat 162, and the movable rod 163 penetrates the connecting seat 162 and extends to the outside, and the side of the movable rod 163 close to the scraping rod 161 is fixedly connected to the surface of the scraping rod 161, and a convex ring 164 is fixedly connected to the outer surface of the movable rod 163, and the convex ring 164 is arranged inside the connecting seat 162, and a spring 2 165 is sleeved on the outer side of the movable rod 163, and the spring 2 165 is arranged inside the connecting seat 162; by setting the scraping assembly 16, specifically, the rotating shaft 143 will drive the connecting seat 162 to rotate together, and the movable rod 163 will drive the scraping rod 161 to rotate together, so that the material attached to the inner wall of the mixing box 1 can be cleaned, the mixing effect can be improved, the culture solution can be mixed more fully, and at the same time it can help discharge.
[0029] A plurality of leakage holes 166 are provided at the bottom of the connecting seat 162. The side of the spring 165 close to the convex ring 164 is fixedly connected to the surface of the convex ring 164, and the side of the spring 165 away from the convex ring 164 is fixedly connected to the inner wall of the connecting seat 162. The elastic action of the spring 165 can make the scraper rod 161 close to the inner wall of the mixing box 1, and this method can improve the cleaning effect of the scraper rod 161.
[0030] A discharge pipe 2 is fixedly connected to the bottom of the mixing box 1, a valve 21 is provided on the outside of the discharge pipe 2, and a feed hopper 13 is fixedly connected to the left side of the top of the mixing box 1; when mixing is in progress, the valve 21 is in a state of sealing the discharge pipe 2, and when discharge is required, the valve 21 is opened to discharge the culture solution from the discharge pipe 2.
[0031] A specific application of this embodiment is:
[0032] The material to be mixed is put into the mixing box 1 from the feed hopper 13. At this time, the valve 21 is in a state of sealing the discharge pipe 2. Then, the motor 111 is started to drive the right belt pulley 431 to rotate. The right belt pulley 431 drives the left belt pulley 431 to rotate through the belt, and the left belt pulley 431 drives the rotating shaft 143 to rotate together, and the tripod 141 rotates together, and the stirring rod 142 mixes the materials. During the mixing process, the lifting plate 15 is in the upper position, and the collision block 152 will not contact the hemispherical protrusion 411. At the same time, the rotating shaft 143 will drive the connecting seat 162 to rotate together, and the movable rod 163 will drive the scraper rod 161 to rotate together. Because the movable rod 163 pushes the convex ring 164 under the elastic action of the spring 2 165, the convex ring 164 will drive the scraper rod 161 to move through the movable rod 163, so that the scraper rod 161 is in close contact with the inner wall of the mixing box 1. This method can attach to the inner wall of the mixing box 1. The material cleaning can improve the mixing effect and help discharge. When the mixing is completed and the material needs to be discharged, the valve 21 is opened to discharge the culture solution from the discharge pipe 2. At the same time, the electric push rod 12 is started to drive the lifting plate 15 to move downward, so that the collision block 152 moves to the top of the tripod 141 and then stops. At this time, when the tripod 141 rotates, the hemispherical protrusion 411 contacts the collision block 152 and a collision effect occurs, and the collision block 152 is pushed upward. The collision block 152 squeezes the spring 154. After the hemispherical protrusion 411 leaves the collision block 152, the collision block 152 is reset under the elastic action of the spring 154. This reciprocating motion can make the tripod 141 vibrate continuously, thereby cleaning the culture solution attached to the tripod 141 and the stirring rod 142. The unloading rate is also improved by scraping with the vibration and the scraper 161, and the culture solution entering the connecting seat 162 will be discharged from the leakage hole 166 by vibration.
[0033] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.
[0034] The preferred embodiments of the utility model disclosed above are only used to help explain the utility model. The preferred embodiments do not describe all the details in detail, nor do they limit the utility model to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the utility model, so that technicians in the relevant technical field can well understand and use the utility model. The utility model is limited only by the claims and their full scope and equivalents.
Claims
1. A culture medium mixing device for culturing mesenchymal stem cells, comprising a mixing box (1), a fixing seat (11) fixedly connected to the top of the mixing box (1), and an electric push rod (12) fixedly connected to the rear position of the top of the mixing box (1), characterized in that: A stirring assembly (14) is arranged inside the mixing box (1), and the stirring assembly (14) comprises a tripod (141). Three stirring rods (142) are fixedly connected to the bottom of the tripod (141), and three hemispherical protrusions (411) are fixedly connected to the top of the tripod (141). A lifting plate (15) is arranged above the tripod (141), and the top of the lifting plate (15) is fixedly connected to the bottom output end of the electric push rod (12). Three fixed blocks (151) are fixedly connected inside the lifting plate (15), and a collision block (152) is slidably connected inside the bottom of the fixed block (151). The collision block (152) penetrates the fixed block (151) and extends to the outside. A spring 1 (154) is fixedly connected to the top of the collision block (152), and the top of the spring 1 (154) is fixedly connected to the top of the inner wall of the fixed block (151).
2. The culture medium mixing device for culturing mesenchymal stem cells according to claim 1, characterized in that: The top of the fixed seat (11) is fixedly connected to a motor (111), the center of the tripod (141) is fixedly connected to a rotating shaft (143), the top of the rotating shaft (143) passes through the mixing box (1) and extends to the outside, the rotating shaft (143) is rotatably connected to the mixing box (1), the top of the rotating shaft (143) and the bottom output end of the motor (111) are both fixedly connected to a belt pulley (431), and the two belt pulleys (431) are connected via a belt drive.
3. The culture medium mixing device for culturing mesenchymal stem cells according to claim 2, characterized in that: The lifting plate (15) is in the shape of a ring, the bottom of the collision block (152) is in the shape of a hemisphere, a stabilizing rod (153) is slidably connected inside the collision block (152), and the top of the stabilizing rod (153) is fixedly connected to the top of the inner wall of the fixing block (151).
4. The culture medium mixing device for culturing mesenchymal stem cells according to claim 3, characterized in that: A scraping assembly (16) is arranged below the tripod (141), and the scraping assembly (16) comprises a scraping rod (161), the outer side of the scraping rod (161) contacts the inner wall of the mixing box (1), the bottom of the rotating shaft (143) is fixedly connected to a connecting seat (162), the interior of the connecting seat (162) is slidably connected to a movable rod (163), the movable rod (163) penetrates the connecting seat (162) and extends to the outside, and the side of the movable rod (163) close to the scraping rod (161) is fixedly connected to the surface of the scraping rod (161).
5. The culture medium mixing device for culturing mesenchymal stem cells according to claim 4, characterized in that: A convex ring (164) is fixedly connected to the outer surface of the movable rod (163), and the convex ring (164) is arranged inside the connecting seat (162). A second spring (165) is sleeved on the outer side of the movable rod (163), and the second spring (165) is arranged inside the connecting seat (162).
6. The culture medium mixing device for culturing mesenchymal stem cells according to claim 5, characterized in that: A plurality of leak holes (166) are provided at the bottom of the connection seat (162); a side of the second spring (165) close to the convex ring (164) is fixedly connected to the surface of the convex ring (164); and a side of the second spring (165) away from the convex ring (164) is fixedly connected to the inner wall of the connection seat (162).
7. The culture medium mixing device for culturing mesenchymal stem cells according to claim 5, characterized in that: A discharge pipe (2) is fixedly connected to the bottom of the mixing box (1), a valve (21) is arranged on the outside of the discharge pipe (2), and a feed hopper (13) is fixedly connected to the left side of the top of the mixing box (1).