Test tube of flow cytometer

Abstract

The utility model relates to the technical field of test tubes, and discloses a test tube of a flow cytometer, which comprises a test tube main body, a round hole is arranged at the top of one side of the test tube main body, a diaphragm is fixedly connected inside the round hole, a fixing mechanism is fixedly connected to one side of the round hole, a silicone tube is fixedly connected to one side of the fixing mechanism, and the silicone tube is fixedly connected to the other side of the test tube main body. The bottom of the test tube main body is integrally connected with a limiting shell, and the interior of the limiting shell is fixedly connected with an isolation mechanism. According to the test tube of the flow cytometer, through the arrangement of the diaphragm, the fixing mechanism and the silicone tube, the piercing device is rotated until the piercing device is rotationally fixed in the threaded sleeve, so that the other side of the piercing device can pierce the diaphragm, and then the silicone tube is extruded, so that washing-free hemolysin in the silicone tube can flow into the test tube main body through the fixing mechanism; the preloading of the washing-free hemolysin is facilitated, the manual addition of the washing-free hemolysin is not needed, and the operation complexity and the time cost of the experiment are reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of test tubes, in particular to a test tube of a flow cytometer. Background Art

[0002] Flow cytometer tubes are small tubes or containers used to mix the sample to be tested (usually a suspension containing cells) and related reagents (such as antibodies, dyes, etc.) and analyze them in the flow cytometer. These tubes are usually made of transparent materials such as polypropylene or polycarbonate to allow light to penetrate, allowing the optical system to detect the fluorescence or scattering signals of the cells.

[0003] However, most of the current flow cytometer tubes are coated with antibody coatings on the bottom of the flow cytometer tubes when in use, but there is no structure to isolate the antibody coating. Due to the lack of isolation and protection mechanism, the antibody coating on the bottom of the tube may be affected by external factors, such as contamination from the environment or operation, resulting in reduced repeatability of the experiment.

[0004] A test tube for a flow cytometer disclosed in the announcement number CN215140057U includes a tube body of the test tube, a placement ring, and a filter membrane arranged in the placement ring. A fixed ring is coaxially fixedly arranged on the tube mouth of the tube body, the placement ring is placed on the fixed ring, and the filter membrane is located in the fixed ring. The application has the effect of improving work efficiency and reducing the labor of staff.

[0005] Although the above patent has achieved the effect of improving work efficiency and reducing staff labor; however, the device lacks a pre-installed wash-free hemolysin structure and requires manual addition of hemolysin, which increases the operational complexity and time cost of the experiment.

[0006] Therefore, it is necessary to propose a flow cytometer test tube to solve the above problems. Utility Model Content

[0007] 1. Technical issues to be solved

[0008] The technical problem solved by the utility model is to provide a flow cytometer test tube which is highly practical, can be simply operated and has a relatively simple structure, thereby solving the problems raised in the above-mentioned background technology of lack of isolation and protection mechanism, the antibody coating at the bottom of the test tube being affected by external factors and lack of pre-installed wash-free hemolysin structure.

[0009] (II) Technical solution

[0010] To achieve the above objectives, the utility model is implemented through the following technical solutions: a test tube for a flow cytometer, comprising a test tube body, a circular hole is opened on the top of one side of the test tube body, a diaphragm is fixedly connected to the inside of the circular hole, a fixing mechanism is fixedly connected to one side of the circular hole, a silicone tube is fixedly connected to one side of the fixing mechanism, a limiting shell is integrally connected to the bottom of the test tube body, an isolation mechanism is fixedly connected to the inside of the limiting shell, a rotating groove is opened on the bottom of the limiting shell, the internal rotation of the rotating groove is connected to the limiting mechanism, a protective mechanism is snap-connected to the top of the test tube body, and a supporting mechanism is snap-connected to the outside of the limiting shell.

[0011] As a further solution of the utility model, the fixing mechanism includes a threaded sleeve fixedly connected to one side of the circular hole, the internal thread of the threaded sleeve is connected to a piercer, one side of the piercer is fixedly connected to the other side of the silicone tube, and the piercer serves to facilitate piercing the diaphragm, so that the wash-free hemolysin in the silicone tube can flow into the test tube body.

[0012] As a further solution of the utility model, the isolation mechanism includes a mesh plate A fixedly connected to the inside of the limiting shell, and the bottom of the mesh plate A is overlapped with a mesh plate B, and the mesh plate B plays the role of protecting the antibody coating of the bottom wall of the container.

[0013] As a further solution of the utility model, the limiting mechanism includes a receiving ring rotatably connected to the inside of the rotating groove, the bottom of the receiving ring is fixedly connected to a vessel, the inner top wall of the vessel is fixedly connected to the periphery of the mesh plate B, and the vessel serves to limit the antibody coating.

[0014] As a further solution of the utility model, the protection mechanism includes a plug that is snap-connected to the top of the test tube body, and the top of the plug is integrally connected with a plug cover. The plug serves to protect the sample inside the test tube body to avoid contamination by sample overflow.

[0015] As a further solution of the utility model, glue is bonded to the front of the test tube body, and a sticker is bonded to the front of the glue. The sticker serves to facilitate writing of the sample type, which is convenient for operators to identify.

[0016] (III) Beneficial effects

[0017] The utility model provides a test tube for a flow cytometer, which has the following beneficial effects:

[0018] 1. The test tube of the flow cytometer is provided with a diaphragm, a fixing mechanism and a silicone tube. The piercer is rotated until the piercer is rotated and fixed inside the threaded sleeve, so that the other side of the piercer can pierce the diaphragm, and then the silicone tube is squeezed so that the wash-free hemolysin in the silicone tube can flow into the test tube body through the fixing mechanism, which is conducive to pre-installing the wash-free hemolysin and does not need to be manually added. The operational complexity and time cost of the experiment are reduced.

[0019] 2. The test tube of the flow cytometer is provided with a limiting shell, an isolating mechanism and a limiting mechanism. When the experiment is set up, the vessel drives the mesh plate B to rotate under the action of the receiving ring and the limiting shell, so that the holes opened on the surface of the mesh plate B are aligned with the holes opened on the surface of the mesh plate A, and the mixture is placed in the test tube body. The mixture is mixed with the antibody coating through the aligned holes. In these mixtures, the flow antibodies of CD interact with the cells in the sample to be tested, and then the mixture is analyzed and detected by the flow cytometer, so as to avoid the antibody coating at the bottom of the vessel being affected by external factors, such as pollution from the environment or operation, which leads to a decrease in the repeatability of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0021] Figure 2 This is a schematic diagram of the fixing mechanism structure of the utility model;

[0022] Figure 3 This is a schematic diagram of the structure of the isolation mechanism and the limiting mechanism of the utility model;

[0023] Figure 4 This is a schematic diagram of the support mechanism structure of the utility model;

[0024] Figure 5 This is a schematic diagram of the structure of the powerful suction cup of the utility model.

[0025] In the figure: 1. test tube body; 2. diaphragm; 3. fixing mechanism; 301. threaded sleeve; 302. piercer; 4. silicone tube; 5. limiting shell; 6. isolating mechanism; 601. mesh plate A; 602. mesh plate B; 7. limiting mechanism; 701. receiving ring; 702. vessel; 8. protective mechanism; 801. plug; 802. plug cover; 9. supporting mechanism; 901. limiting ring; 902. supporting leg; 903. base; 904. plug rod; 905. receiving block; 906. strong suction cup; 10. sticker. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0027] See also Figures 1 to 5 The utility model provides a technical solution: a test tube for a flow cytometer, comprising a test tube body 1, a round hole is opened on the top of one side of the test tube body 1, a diaphragm 2 is fixedly connected inside the round hole, a fixing mechanism 3 is fixedly connected on one side of the round hole, a silicone tube 4 is fixedly connected on one side of the fixing mechanism 3, and the setting of the diaphragm 2, the fixing mechanism 3 and the silicone tube 4 facilitates the pre-installation of the wash-free hemolysin, and there is no need to manually add the wash-free hemolysin, thereby reducing the operational complexity and time cost of the experiment, the bottom of the test tube body 1 is integrally connected with a limiting shell 5, the inside of the limiting shell 5 is fixedly connected with an isolation mechanism 6, the bottom of the limiting shell 5 is opened with a rotating groove, and the inside of the rotating groove is rotatably connected with the limiting mechanism 7, and the setting of the limiting shell 5, the isolation mechanism 6 and the limiting mechanism 7 prevents the antibody coating at the bottom of the vessel 702 from being affected by external factors, such as pollution from the environment or operation, thereby reducing the repeatability of the experiment, the top of the test tube body 1 is snap-connected with a protective mechanism 8, and the outside of the limiting shell 5 is snap-connected with a supporting mechanism 9;

[0028] See also Figure 2 The fixing mechanism 3 includes a threaded sleeve 301 fixedly connected to one side of the circular hole, the internal thread of the threaded sleeve 301 is connected to a piercer 302, one side of the piercer 302 is fixedly connected to the other side of the silicone tube 4, and the piercer 302 serves to facilitate piercing the diaphragm 2, so that the wash-free hemolysin in the silicone tube 4 can flow into the test tube body 1.

[0029] See also Figure 3 The isolation mechanism 6 includes a mesh plate A601 fixedly connected to the inside of the limiting shell 5, and a mesh plate B602 is overlapped at the bottom of the mesh plate A601, and the mesh plate B602 plays the role of antibody coating on the inner bottom wall of the protective container 702.

[0030] See also Figure 3 The limiting mechanism 7 includes a receiving ring 701 rotatably connected to the inside of the rotating groove, a container 702 is fixedly connected to the bottom of the receiving ring 701, and the inner top wall of the container 702 is fixedly connected to the periphery of the mesh plate B602. The container 702 plays a role in limiting the antibody coating.

[0031] See also Figure 3The protection mechanism 8 includes a plug 801 snap-fitted to the top of the test tube body 1, and a plug cover 802 is integrally connected to the top of the plug 801. The plug 801 plays a role in protecting the sample inside the test tube body 1 to prevent it from being contaminated by sample overflow.

[0032] See also Figure 1 The front of the test tube body 1 is bonded with glue, and the front of the glue is bonded with a sticker 10, which serves to facilitate writing of the sample type and facilitate identification by operators.

[0033] Embodiment 1:

[0034] See also Figure 4 The supporting mechanism 9 includes a limiting ring 901 which is snap-fitted to the outside of the limiting shell 5. The bottom of the limiting ring 901 is snap-fitted with legs 902. The bottoms of the four legs 902 are fixedly connected with a group of bases 903. The limiting ring 901 is fixed on the desktop under the action of the four groups of legs 902 and the group of bases 903. Then the test tube body 1 is placed on the limiting ring 901, which is convenient for limiting the test tube body 1, facilitating better experimental operation and improving practicality.

[0035] Embodiment 2:

[0036] See also Figure 5 The supporting mechanism 9 includes four groups of plug rods 904 which are snap-fitted to the outside of the limiting shell 5. The bottom of the four plug rods 904 is integrally connected with a group of receiving blocks 905. The bottom of the receiving blocks 905 is bonded with a strong suction cup 906. The strong suction cup 906 is pressed to generate negative pressure. At this time, the limiting ring 901 is firmly fixed on the desktop. The operator can insert the test tube body 1 on the limiting ring 901 to fix it, so as to avoid the test tube body 1 from shifting during the experiment.

[0037] In the utility model, the working steps of the device are as follows:

[0038] Step 1: Rotate the piercer 302 until the piercer 302 is rotated and fixed inside the threaded sleeve 301, so that the other side of the piercer 302 can pierce the diaphragm 2, and then squeeze the silicone tube 4 so that the wash-free hemolysin in the silicone tube 4 can flow into the test tube body 1 through the fixing mechanism 3;

[0039] The second step: during the experimental setup, the vessel 702 drives the mesh plate B602 to rotate under the action of the receiving ring 701 and the limiting shell 5, so that the holes opened on the surface of the mesh plate B602 are aligned with the holes opened on the surface of the mesh plate A601, and the mixture is placed in the test tube body 1. The mixture is mixed with the antibody coating through the aligned holes. In these mixtures, the flow antibody of CD161 interacts with the cells in the sample to be tested, and then is analyzed and detected by the flow cytometer.

[0040] It should be noted that the equipment structure and drawings of the utility model mainly describe the principle of the utility model. In terms of the technology of the design principle, the settings of the power mechanism, power supply system and control system of the device are not fully described. On the premise that the technical personnel in this field understand the principle of the above utility model, the details of the power mechanism, power supply system and control system can be clearly known. The control method of the application document is automatic control through a controller, and the control circuit of the controller can be realized by simple programming by the technical personnel in this field;

[0041] The standard parts used therein can all be purchased from the market and can be customized according to the instructions and drawings. The specific connection methods of each part adopt conventional means such as mature bolts, rivets, welding, etc. in the prior art. The machinery, parts and equipment all adopt conventional models in the prior art, and the structures and principles of the components known to technical personnel in this field can be known by these technical personnel through technical manuals or through conventional experimental methods.

[0042] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A test tube for a flow cytometer, comprising a test tube body (1), characterized in that: A circular hole is provided at the top of one side of the test tube body (1), a diaphragm (2) is fixedly connected inside the circular hole, a fixing mechanism (3) is fixedly connected to one side of the circular hole, a silicone tube (4) is fixedly connected to one side of the fixing mechanism (3), a limiting shell (5) is integrally connected to the bottom of the test tube body (1), an isolation mechanism (6) is fixedly connected inside the limiting shell (5), a rotating groove is provided at the bottom of the limiting shell (5), the internal rotation of the rotating groove is connected to the limiting mechanism (7), a protective mechanism (8) is snap-fitted to the top of the test tube body (1), and a supporting mechanism (9) is snap-fitted to the outside of the limiting shell (5).

2. A flow cytometer test tube according to claim 1, characterized in that: The fixing mechanism (3) comprises a threaded sleeve (301) fixedly connected to one side of the circular hole, the internal thread of the threaded sleeve (301) is connected to a piercer (302), and one side of the piercer (302) is fixedly connected to the other side of the silicone tube (4).

3. A flow cytometer test tube according to claim 1, characterized in that: The isolation mechanism (6) comprises a mesh plate A (601) fixedly connected to the interior of the limiting shell (5), and a mesh plate B (602) is overlapped at the bottom of the mesh plate A (601).

4. A flow cytometer test tube according to claim 1, characterized in that: The limiting mechanism (7) comprises a receiving ring (701) rotatably connected to the inside of the rotating groove, the bottom of the receiving ring (701) is fixedly connected to a container (702), and the inner top wall of the container (702) is fixedly connected to the periphery of the mesh plate B (602).

5. A flow cytometer test tube according to claim 1, characterized in that: The protection mechanism (8) comprises a plug (801) snap-fitted to the top of the test tube body (1), and a plug cover (802) is integrally connected to the top of the plug (801).

6. A flow cytometer test tube according to claim 1, characterized in that: The front side of the test tube body (1) is bonded with glue, and the front side of the glue is bonded with a sticker (10).

Images