Small microplate reader based on dichroscope

Abstract

The utility model discloses a small microplate reader based on dichroscopes, which comprises a lighting device, a receiving device and a detection device, the lighting device comprises four emission sources with different wavelengths and three dichroscopes with different transmittances, and the four emission sources can respectively generate a plurality of light beam paths with different wavelengths through the dichroscopes with different transmittances; the light beams serve as transmission light beam paths to penetrate through the receiving device to reach each detector unit of the detection device, the receiving device is used for placing the microwell plate, and cavities with corresponding positions and quantity are formed in the microwell plate corresponding to the positions and quantity of the transmission light beam paths and are used for containing samples to be detected. According to the microplate reader disclosed by the utility model, the inspection of different samples by one microplate reader with different wavelengths can be realized through the dichroscope, so that the influence of stray light on the inspection is reduced to the minimum, at least four light beams with different wavelengths can be provided, and complicated processing and larger volume are not needed; the microplate reader is miniaturized, and the manufacturing cost is saved.

Description

Technical Field

[0001] The utility model relates to the technical field of microplate readers, in particular to a small microplate reader based on a dichroic mirror. Background Art

[0002] The enzyme-linked immunosorbent assay (ELISA) is a professional instrument for reading and analyzing the results of ELISA experiments. It is used to detect absorbance values ​​in ELISA reactions. Its core is a colorimeter, which uses colorimetry to analyze the content of antigens or antibodies. The light waves emitted by the light source lamp are converted into a monochromatic light through a filter or a monochromator and enter the sample to be tested in the plastic microplate. Part of the monochromatic light is absorbed by the sample, and the other part is irradiated through the sample to the photodetector. The photodetector converts the light signal of the sample to be tested into a corresponding electrical signal. After pre-amplification, logarithmic amplification, analog-to-digital conversion and other signal processing, the electrical signal is sent to the microprocessor for data processing and calculation, and finally the display shows the result. The ELISA can detect target samples quickly, with high precision and strong specificity, and is widely used in clinical testing, biological research, environmental science and other fields. When medical staff conduct ELISA tests, an ELISA reader is a very necessary device. At present, medical staff generally use large ELISA readers. However, this device is cumbersome to use, and when medical staff conduct ELISA tests, this large ELISA reader is usually bulky and difficult to move. It cannot meet the various necessary requirements of medical staff during ELISA tests, thus delaying the inspection process and bringing great work difficulties to medical staff. Therefore, many new ELISA readers have been invented. Chinese patent CN 210953795 U "A new type of microplate reader" combines a digital signal processor DSP with a temperature sensor to provide an innovative microplate reader that is easy to carry, flexible to use, has low standard limits, short response time, high sensitivity, good stability, simple structure and low cost when medical staff perform microplate detection; In addition, Chinese patent CN 210953795 U "A microplate reader" sets the microplate reader body in a placement slot, sets a number of removable buffer air bags between the microplate reader body and the placement slot, and sets an anti-skid pad on the top of the buffer air bag, designing a microplate reader with shockproof function; Chinese patent CN 211086073 U "Microplate reader metering device" solves the technical problem of providing a microplate reader metering device, which can automatically add liquid, is easy to operate and easy to clean. Usually, microplate reader devices are large, expensive and time-consuming. These devices have a mobile emission source and detector for generating transmitted light for measuring microplates. However, such a mechanism requires additional space and leads to additional costs for equipment manufacturing, and mechanical failures can also lead to equipment failures. In addition, the microplate reader still has disadvantages such as complex operation, susceptibility to environmental influences and high prices. In addition, the microplate reader can only measure light beams at one wavelength at a time, and the design structure is relatively complex and large in size.

[0003] Therefore, many researchers have made improvements based on the above-mentioned microplate reader and designed more new microplate readers with complete functions and smaller size. U.S. Patent Document EN102018111033A1 "Transmission Device for Checking Samples in Microplate Holes and Method for Checking Samples in Microplate Holes by Transmission" includes an illumination device and a detection device, and a space is formed between the illumination device and the detection device, which is used to receive the microplate. The emission source in the illumination device is used to generate emission light, and divide the emission light into a plurality of partial beam paths as transmission beam paths extending through the intermediate space to the respective detector units of the detection device, wherein the light signal incident along the transmission beam path in the first time period is detected by the detector unit for each transmission beam path. The illumination device includes an optical mixer, which is designed to homogenize the emission light generated by the emission source and distribute it to the partial beam paths with uniform intensity, and each beam path runs in a light guide plate, and the light guide plate in which the transmission beam path runs is configured to irradiate the partial emission light from the optical mixer to the respective emission ports of the illumination device. This patent enables the detection of light beams at multiple wavelengths without moving the emission source, thereby achieving a compact and cost-effective design of the transmission device. It can realize the simple operation of the microplate reader, low price and space-saving inspection of samples in microplates.

[0004] However, this technical solution still has shortcomings. The lighting device has four commonly used wavelengths of light sources set as four channels of emission sources, which still has problems such as large size, complex structure and difficulty in processing. In addition, the device is difficult to process and realize emission sources at more wavelengths. Therefore, it is of great significance to develop a solution for an ELISA device that is easy to process and has a simple structure. Utility Model Content

[0005] In order to solve the above technical problems, the utility model designs a small-sized enzyme marker based on a dichroic mirror.

[0006] The utility model adopts the following technical solutions:

[0007] A small-sized microplate reader based on a dichroic mirror comprises an illuminating device, a receiving device and a detecting device. The illuminating device comprises 4 emission sources of different wavelengths and 3 dichroic mirrors of different transmittances. The 4 emission sources can respectively generate multiple light beam paths of different wavelengths through the dichroic mirrors of different transmittances, and the multiple light beam paths are used as transmission light beam paths to pass through the receiving device to reach various detector units of the detecting device. The receiving device is used to place a microplate. The microplate is provided with cavities of corresponding positions and numbers corresponding to the positions and numbers of the transmission light beam paths, and are used to hold samples to be detected. The detecting device is used to simultaneously measure the light signals of each transmission light beam path reaching the detecting device.

[0008] Preferably, the lighting device divides the emission light generated by the emission source into at least 96 beam paths, of which 96 beam paths are transmission beam paths, corresponding to samples in all holes of the 96-hole microplate passing through to reach 96 detector units of the detection device for detection.

[0009] Preferably, the lighting device can generate emission light using an emission source of corresponding wavelength according to the requirements of the sample to be detected.

[0010] Preferably, the four emission sources in the lighting device are respectively a 620nm red light source, a 492nm green light source, a 450nm blue light source and a 405nm violet light source.

[0011] Preferably, the lighting device comprises three dichroic mirrors with different transmittances, the first dichroic mirror being used to transmit red light with a wavelength of 622-760nm and reflect green light with a wavelength of 492-577nm; the second dichroic mirror being used to transmit red light and green light and reflect blue light with a wavelength of 435-450nm; the third dichroic mirror being used for red light, green light and blue light and reflecting violet light with a wavelength of 390-435nm.

[0012] Preferably, the receiving device is provided with an intermediate space, which is an open measurement structure for inserting a microplate.

[0013] Preferably, at least one of the transmission beam paths is a reference beam path for directing the emitted light to illuminate a reference detector unit in the illumination device.

[0014] Preferably, each detector unit in the detection device comprises at least one detector.

[0015] The beneficial effects of the utility model are as follows: (1) The utility model can realize that an ELISA instrument can inspect different samples with different wavelengths through a dichroic mirror, thereby minimizing the influence of stray light on the inspection, and can provide at least 4 light beams of different wavelengths without complicated processing and large volume, thereby realizing the miniaturization of the ELISA instrument and saving manufacturing costs. In addition, the measurement operation of light beams of different wavelengths is simple, and it only needs to turn on the emission sources of different wavelengths; (2) The ELISA instrument based on the dichroic mirror of the utility model can use the corresponding emission source to divide the light beam into 96 parts to illuminate 96 cavities according to the needs of the sample to be tested, without moving the emission source. Compared with the device in which the emission source and the detector are moved by a mechanical system, by dividing the emission light into multiple transmission light beam paths, the transmission device is made compact, cost is saved and efficiency is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a structural schematic diagram of the utility model;

[0017] Figure 2 It is a structural schematic diagram of the microporous plate in the utility model;

[0018] Figure 3 It is a structural schematic diagram of the lighting device in the utility model;

[0019] Figure 4 It is a structural schematic diagram of the detection device in the utility model;

[0020] In the figure: 1. transmission device, 2. lighting device, 3. detection device, 4. intermediate space, 5. microplate, 30. detector unit, 50. cavity. DETAILED DESCRIPTION

[0021] The technical solution of the utility model is further described in detail below through specific embodiments and in conjunction with the accompanying drawings:

[0022] Example: Figure 1 As shown, a small-sized microplate reader based on a dichroic mirror comprises a transmission device 1, wherein the transmission device 1 comprises an illumination device 2, a receiving device and a detection device 3, wherein the receiving device is located between the illumination device 2 and the detection device 3 and has an intermediate space 4 with a rectangular opening. The intermediate space 4 is designed to be inserted into a microplate 5, such as Figure 2 Therefore, the size of the intermediate space 4 is substantially consistent with the size of the microplate 5, so that the transmission device 1 has a compact structure.

[0023] like Figure 2 As shown, the microplate 5 of this embodiment has ninety-six cavities 50, of which only one cavity 50 is provided with a reference mark. Before the microplate 5 is inserted into the intermediate space 4, the samples to be tested are placed in these cavities 50.

[0024] like Figure 3 As shown, the microplate reader based on the dichroic mirror of this embodiment includes an illumination device 2, which is composed of 4 emission sources of different wavelengths and 3 dichroic mirrors of different transmittances. The emission source of the corresponding wavelength can be turned on according to the sample to be detected, and the emission light generated by the emission source can be divided into 96 partial beam paths as the transmission beam path by respectively generating the beam paths of the required wavelength through the dichroic mirrors of different transmittances.

[0025] like Figure 4As shown, the microplate reader based on the dichroic mirror of this embodiment includes a detection device 3, which is arranged below the lighting device 2 and the inserted microplate 5. The surface area of ​​the detection device 3 corresponds to the surface of the inserted microplate 5. The emitted light is divided into 96 partial beam paths as transmission beam paths to reach the detector unit 30 of the detection device, which can advantageously detect samples in 96 cavities respectively.

[0026] The above-described embodiment is only a preferred solution of the present invention and does not limit the present invention in any form. There are other variations and modifications without exceeding the technical solution described in the claims.

Claims

1. A small-sized microplate reader based on a dichroic mirror, comprising an illumination device, a receiving device and a detection device, characterized in that: The lighting device includes four emission sources with different wavelengths and three dichroic mirrors with different transmittances. The four emission sources can respectively generate multiple light beam paths with different wavelengths through dichroic mirrors with different transmittances, and the multiple light beam paths pass through a receiving device as transmission light beam paths to reach various detector units of a detection device. The receiving device is used to place a microplate, and cavities with corresponding positions and numbers are arranged on the microplate corresponding to the positions and numbers of the transmission light beam paths for holding samples to be detected. The detection device is used to simultaneously measure the light signals of each transmission light beam path that reaches the detection device.

2. A small-sized microplate reader based on a dichroic mirror according to claim 1, characterized in that: The lighting device divides the emission light generated by the emission source into at least 96 beam paths, of which 96 beam paths are transmission beam paths, corresponding to samples in all holes of the 96-hole microplate, and reach 96 detector units of the detection device for detection.

3. A small-sized microplate reader based on a dichroic mirror according to claim 1, characterized in that: The lighting device can generate emission light using an emission source of corresponding wavelength according to the requirements of the sample to be detected.

4. A small-sized microplate reader based on a dichroic mirror according to claim 1, characterized in that: The four emission sources in the lighting device are respectively a 620nm red light source, a 492nm green light source, a 450nm blue light source and a 405nm violet light source.

5. A small-sized microplate reader based on a dichroic mirror according to claim 1, characterized in that: The lighting device has three dichroic mirrors with different transmittances. The first dichroic mirror is used to transmit red light with a wavelength of 622-760nm and reflect green light with a wavelength of 492-577nm; the second dichroic mirror is used to transmit red light and green light and reflect blue light with a wavelength of 435-450nm; the third dichroic mirror is used for red light, green light and blue light and reflects violet light with a wavelength of 390-435nm.

6. A small-sized microplate reader based on a dichroic mirror according to claim 1, characterized in that: The receiving device is provided with an intermediate space, which is an open measurement structure for inserting and placing the microplate.

7. A small-sized microplate reader based on a dichroic mirror according to claim 1, characterized in that: At least one of the transmission beam paths is a reference beam path for directing emitted light to illuminate a reference detector unit in the illumination device.

8. A small-sized microplate reader based on a dichroic mirror according to claim 1, characterized in that: Each detector unit in the detection device comprises at least one detector.

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