Precipitation integrated biological enzyme treatment device

Abstract

The utility model provides a precipitation integrated biological enzyme treatment device, and belongs to the technical field of purification and separation equipment. Comprising a first tank body, a top cover is arranged at the top of the first tank body, a mounting base is coaxially and rotatably arranged in the first tank body, a second tank body is detachably connected to the mounting base, a stirring assembly, a liquid inlet pipe, a feeding port and an extraction assembly are arranged on the top cover, a plurality of liquid drainage holes are formed in the side wall of the second tank body in a penetrating mode, and filtering layers are arranged in the liquid drainage holes; a plugging assembly for sealing the filter layer is arranged in the second tank body, and a liquid discharge pipe is arranged at the bottom of the first tank body. And carrying out precipitation treatment in the second tank body, after the treatment is completed, extracting supernatant liquid, then opening the plugging assembly, driving the second tank body to rotate, and carrying out centrifugal separation to obtain separated enzyme-containing precipitates. The method has the effect of improving the yield of the enzyme preparation.

Description

Technical Field

[0001] This utility model relates to the field of purification and separation equipment technology, and in particular to an integrated precipitation and separation bio-enzyme treatment device. Background Technology

[0002] Enzyme precipitation is mainly used for the separation of biochemical products, especially in the separation and extraction of proteins (enzymes). It not only simplifies the production process and reduces production costs, but also improves the stability and purity of the products. Specialized purification and separation equipment, such as enzyme precipitation tanks, is required during enzyme precipitation.

[0003] Patent CN218860699U discloses a biological enzyme precipitation tank, including a tank body with a stirring mechanism inside. An extraction tube is vertically inserted through the tank body, with the top opening of the extraction tube connected to the inlet of a water pump. The extraction tube is vertically slidably mounted on the tank body, and a driving mechanism is provided on the tank body to drive the extraction tube to slide vertically. A conical extraction head is provided at the bottom end of the extraction tube, with the tip of the extraction head pointing vertically downwards. An extraction port communicating with the extraction tank is opened at the upper end of the extraction head.

[0004] After the supernatant in the sedimentation tank is extracted, the precipitate in the sedimentation tank needs to be removed and centrifuged to obtain a relatively pure enzyme preparation. During the process of removing the precipitate from the sedimentation tank and centrifuging again, there is a loss of precipitate, which affects the yield of enzyme preparation. Utility Model Content

[0005] In view of the above problems, this utility model provides an integrated precipitation and separation bio-enzyme treatment device.

[0006] To achieve the above-mentioned objectives, the technical solution adopted by this utility model is as follows:

[0007] An integrated precipitation and precipitation bio-enzyme treatment device is provided, comprising a first tank with a top opening, a top cover for opening and closing the first tank opening, a mounting base coaxially rotatably mounted inside the first tank, a driving component for rotating the mounting base at the bottom of the first tank, a second tank detachably connected to the mounting base, the second tank having a top opening, a stirring assembly extending into the second tank mounted on the top cover, an inlet pipe and a feeding port communicating with the second tank mounted on the top cover, an extraction assembly for extracting the clear liquid from the second tank mounted on the top cover, several drain holes penetrating through the side wall of the second tank, a filter layer disposed within the drain holes, a sealing assembly for sealing the filter layer disposed inside the second tank, and a drain pipe disposed at the bottom of the first tank.

[0008] Furthermore, the stirring assembly includes a rotating shaft, the top end of which is rotatably connected to the top cover. Several stirring blades are fixedly arranged on the outer wall of the rotating shaft, and a first drive motor for driving the rotating shaft to rotate is provided on the top cover.

[0009] The extraction assembly includes an extraction tube and a first electric push rod. The extraction tube is vertically slidably mounted on the top cover. The bottom end of the extraction tube is open and extends into the bottom of the top cover. The top end of the extraction tube is connected to an external water pump through a pipe. The first electric push rod is fixedly mounted on the top cover, and the piston rod of the first electric push rod is fixedly connected to the extraction tube.

[0010] Furthermore, the sealing assembly includes a sealing sleeve and a second electric push rod. The sealing sleeve is coaxially slidably disposed inside the second tank, and the second electric push rod is disposed on the second tank. The second electric push rod is used to drive the sealing sleeve to move vertically up and down. The top end of the sealing sleeve is provided with a first sealing ring for sealing contact with the inner side wall of the second tank, and the bottom end of the sealing sleeve is provided with a second sealing ring for sealing contact with the bottom wall of the second tank.

[0011] Furthermore, the mounting base has a polygonal mounting groove, and the bottom of the second tank has a mounting block that matches the size of the mounting groove.

[0012] Furthermore, a limit pin is horizontally slidably installed on the mounting base, and an elastic element is provided inside the mounting base to drive one end of the limit pin into the mounting groove. The limit pin is made of magnetic material, and the end of the limit pin that extends into the mounting groove is one end magnetic pole of the limit pin. A limit groove adapted to the limit pin is opened on the mounting block, and an electromagnet is provided inside the mounting block. The limit pin is disengaged from the limit groove by the magnetic repulsion of the electromagnet.

[0013] Furthermore, a handle is provided on the top of the second tank.

[0014] The beneficial effects of this invention are as follows: After the purified biological enzyme culture medium is introduced into the second tank through the inlet pipe, the precipitation agent is added into the second tank through the feeding port. The culture medium and the precipitation agent are mixed by the stirring component until the precipitation is completed. The extraction component extracts the clear liquid from the upper layer of the second tank, the sealing component is opened, and the second tank is driven to rotate. During the rotation of the second tank, the precipitate in the second tank is centrifuged and separated. The centrifuged liquid is discharged from the drain pipe at the bottom of the first tank. The second tank is then removed, and the separated enzyme-containing precipitate is obtained. This invention integrates the precipitation and centrifugation processes, reduces the loss of precipitate during the transfer process, and increases the yield of enzyme preparations. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of the bio-enzyme treatment device according to an embodiment of this application.

[0016] Figure 2 This is a schematic diagram of the exploded structure of the bio-enzyme treatment device according to an embodiment of this application.

[0017] Figure 3 This is a cross-sectional view of the second tank and mounting base according to an embodiment of this application.

[0018] Figure 4 for Figure 3 A magnified view of part A in the diagram.

[0019] The components include: 1. First tank body; 11. Top cover; 12. Liquid inlet pipe; 13. Feeding port; 14. Liquid drain pipe; 2. Mounting base; 21. Mounting groove; 22. Limiting pin; 23. Elastic element; 24. Second drive motor; 3. Second tank body; 31. Liquid drain hole; 32. Filter layer; 33. Mounting block; 34. Limiting groove; 35. Electromagnet; 36. Handle; 4. Stirring assembly; 41. Rotating shaft; 42. Stirring blade; 43. First drive motor; 5. Extraction assembly; 51. Extraction tube; 52. First electric push rod; 61. Sealing sleeve; 62. Second electric push rod. Detailed Implementation

[0020] To better understand the above technical solutions, the following will provide a detailed explanation of the technical solutions in conjunction with the accompanying drawings and specific implementation methods.

[0021] This application discloses an integrated precipitation and precipitation bio-enzyme treatment device, referring to... Figure 1 and Figure 2 The system includes a first tank 1 and a second tank 3. The first tank 1 has an opening at the top, and a top cover 11 is detachably mounted on the top of the first tank 1 by bolts. A mounting base 2 is coaxially and rotatably mounted inside the first tank 1 at its bottom, and the second tank 3 is detachably mounted on the mounting base 2. A driving component for rotating the mounting base 2 is provided at the bottom of the first tank 1. The driving component is a second drive motor 24, which is fixed to the bottom of the first tank 1 by bolts. The output shaft of the second drive motor 24 extends into the first tank 1 and is fixedly connected to the mounting base 2. The top opening of the second tank 3 is provided with a stirring assembly 4, an extraction assembly 5, an inlet pipe 12, and a feeding port 13 on the top cover 11. The stirring assembly 4 extends into the second tank 3 from the top of the second tank 3 and is used to stir the culture medium in the second tank 3. The extraction assembly 5 is used to extract the supernatant that has precipitated in the second tank 3. The inlet pipe 12 and the feeding port 13 are used to introduce culture medium into the second tank 3 and to add precipitation reagent into the second tank 3, respectively.

[0022] In this embodiment, the stirring assembly 4 includes a rotating shaft 41, the top end of which is coaxially rotatably connected to the top cover 11. Several stirring blades 42 are fixedly connected to the outer wall of the rotating shaft. A first drive motor 43 is fixedly installed on the top of the top cover 11, and the output shaft of the first drive motor 43 is drively connected to the rotating shaft 41. The extraction assembly 5 includes an extraction tube 51 and a first electric push rod 52. The extraction tube 51 is vertically slidably installed on the top cover 11. The top of the extraction tube 51 is located above the top cover 11 and connected to a water pump via a pipe. The bottom end of the extraction tube 51 is located below the top cover 11 and within the opening of the second tank 3. The first electric push rod 52 is fixedly installed on the top cover 11. The piston rod of the first electric push rod 52 is fixedly connected to the extraction tube 51. As the piston rod of the first electric push rod 52 moves, the extraction tube 51 can descend and insert into the supernatant that has precipitated in the second tank 3, and the supernatant is extracted using a water pump.

[0023] Reference Figure 3 and Figure 4 The second tank 3 has several drainage holes 31 extending through its side wall. A filter layer 32 is installed inside each drainage hole 31. A sealing assembly for sealing the filter layer 32 is also installed inside the second tank 3. A drainage pipe 14 is installed at the bottom of the first tank 1. By rotating the mounting base 2 at the bottom of the first tank 1, the second tank 3 rotates. Using the drainage holes 31 and the filter layer 32 within them, the residual precipitate in the second tank 3 is centrifuged. The centrifuged liquid enters the first tank 1 and is discharged through the drainage pipe 14, leaving only the enzyme preparation in the second tank 3. By removing the second tank 3, the enzyme preparation can be processed centrally.

[0024] A sealing assembly for sealing the filter layer 32 is provided inside the second tank 3 to seal the drain hole 31 during the sedimentation stage. Specifically, the sealing assembly includes a sealing sleeve 61 and a second electric push rod 62. The sealing sleeve 61 is coaxially slidably disposed inside the second tank 3, and the second electric push rod 62 is disposed on the second tank 3. The second electric push rod 62 is used to drive the sealing sleeve to move vertically up and down. The top end of the sealing sleeve 61 is provided with a first sealing ring for sealing contact with the inner wall of the second tank 3, and the bottom end of the sealing sleeve 61 is provided with a second sealing ring for sealing contact with the bottom wall of the second tank 3. During the sedimentation stage, lowering the sealing sleeve 61 can seal the drain hole 31. During the centrifugation stage, raising the sealing sleeve 61 allows the drain hole 31 and the filter layer 32 to be centrifuged.

[0025] In this embodiment, the mounting base 2 has a polygonal mounting groove 21, and the bottom of the second tank 3 has a mounting block 33 that matches the size of the mounting groove 21. When the second tank 3 is installed in the first tank 1, the mounting block 33 cooperates with the mounting groove 21, so that the second tank 3 can rotate when the mounting base 2 rotates.

[0026] Furthermore, a limiting pin 22 is horizontally slidably mounted on the mounting base 2. An elastic element 23 is provided inside the mounting base 2 to drive one end of the limiting pin 22 into the mounting groove 21. A limiting groove 34 adapted to the limiting pin 22 is provided on the mounting block 33. When the mounting block 33 mates with the mounting groove 21, the limiting pin 22 is pushed into the limiting groove 34 by the elastic element 23, effectively preventing the second tank 3 from detaching from the mounting base 2 and improving the stability of their connection. The elastic element 23 is specifically a spring, which is mounted on the mounting base 2, with one end connected to the mounting base 2 and the other end connected to the limiting pin 22.

[0027] In order to facilitate the separation of the second tank 3 from the mounting base 2, in this embodiment, the limiting pin 22 is made of magnetic material. One end of the limiting pin 22 that extends into the mounting groove 21 is the magnetic pole of the limiting pin 22. An electromagnet 35 is provided in the mounting block 33. The electromagnet 35 magnetically repels the limiting pin 22, causing the limiting pin 22 to move into the mounting base 2 against the spring force, thereby separating the limiting pin 22 from the limiting groove 34 and allowing the second tank 3 to be quickly removed from the mounting base 2.

[0028] Furthermore, a handle 36 is fixedly installed on the top of the second tank 3, which allows the second tank 3 to be easily extracted from the first tank 1.

[0029] Those skilled in the art will understand that although preferred embodiments of the present invention have been described, those skilled in the art, once they understand the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments as well as all changes and modifications falling within the scope of the present invention. Clearly, those skilled in the art can make various alterations and modifications to the present invention without departing from its spirit and scope. Thus, if these modifications and modifications of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention also intends to include these modifications and modifications.

Claims

1. A precipitation-sedimentation integrated bio-enzyme treatment device, characterized in that: The system includes a first tank (1) with a top opening, a top cover (11) for opening and closing the opening of the first tank (1), a mounting base (2) rotatably mounted coaxially inside the first tank (1), a driving component for rotating the mounting base (2) at the bottom of the first tank (1), a second tank (3) detachably connected to the mounting base (2), a top opening of the second tank (3), and a stirring assembly (4) extending into the second tank (3) mounted on the top cover (11). The top of the top cover (11) is provided with an inlet pipe (12) and a feeding port (13) that connects to the second tank (3). The top cover (11) is provided with an extraction component (5) for extracting the clear liquid in the second tank (3). Several drain holes (31) are provided through the side wall of the second tank (3). A filter layer (32) is provided in the drain hole (31). A sealing component for sealing the filter layer (32) is provided in the second tank (3). A drain pipe (14) is provided at the bottom of the first tank (1).

2. The integrated precipitation and precipitation bio-enzyme treatment device according to claim 1, characterized in that, The stirring assembly (4) includes a rotating shaft (41), the top end of which is rotatably connected to the top cover (11). Several stirring blades (42) are fixedly arranged on the outer wall of the rotating shaft. A first drive motor (43) for driving the rotating shaft (41) to rotate is provided on the top cover (11). The extraction assembly (5) includes an extraction tube (51) and a first electric push rod (52). The extraction tube (51) is vertically slidably mounted on the top cover (11). The bottom end of the extraction tube (51) is open and extends into the bottom of the top cover (11). The top end of the extraction tube (51) is connected to an external water pump through a pipe. The first electric push rod (52) is fixedly mounted on the top cover (11). The piston rod of the first electric push rod (52) is fixedly connected to the extraction tube (51).

3. The integrated precipitation and precipitation bio-enzyme treatment device according to claim 1, characterized in that, The sealing assembly includes a sealing sleeve (61) and a second electric push rod (62). The sealing sleeve (61) is coaxially slidably disposed inside the second tank (3). The second electric push rod (62) is disposed on the second tank (3) and is used to drive the sealing sleeve to rise and fall vertically. The top end of the sealing sleeve (61) is provided with a first sealing ring for sealing contact with the inner wall of the second tank (3), and the bottom end of the sealing sleeve (61) is provided with a second sealing ring for sealing contact with the inner bottom wall of the second tank (3).

4. The integrated precipitation and precipitation bio-enzyme treatment device according to claim 1, characterized in that, The mounting base (2) has a polygonal mounting groove (21) on its surface, and the bottom of the second tank body (3) is provided with a mounting block (33) that matches the size of the mounting groove (21).

5. The integrated precipitation and precipitation bio-enzyme treatment device according to claim 4, characterized in that, A limiting pin (22) is horizontally slidably provided on the mounting base (2). An elastic element (23) is provided inside the mounting base (2) to drive one end of the limiting pin (22) into the mounting groove (21). The limiting pin (22) is made of magnetic material. One end of the limiting pin (22) that extends into the mounting groove (21) is a magnetic pole of the limiting pin (22). A limiting groove (34) adapted to the limiting pin (22) is provided on the mounting block (33). An electromagnet (35) is provided inside the mounting block (33). The limiting pin (22) is disengaged from the limiting groove (34) by the magnetic repulsion of the electromagnet (35).

6. The integrated precipitation and precipitation bio-enzyme treatment device according to any one of claims 1 to 5, characterized in that, The second tank (3) is provided with a handle (36) on its top.

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