Verticillium dahliae secretory protein generating and collecting device

Abstract

The utility model provides a verticillium dahliae secreted protein generating and collecting device, which relates to the technical field of verticillium dahliae secreted protein and comprises an operating table, a drying machine is arranged at the top of the operating table, a filter component is arranged at the position, located at the lower end of the drying machine, of the operating table, and the filter component comprises a filter box. A handle is connected to the front surface of the filter box, inserting grooves are formed in the outer surfaces of the two sides of the handle, a fixing box is connected to the surface, close to the handle, of the operation table, a two-way screw is inserted into the fixing box, and the two ends of the two-way screw are connected with a bearing and a handle correspondingly; and moving blocks are connected to the surfaces of the two sides of the two-way screw rod. According to the verticillium dahliae secretory protein drying device, after verticillium dahliae secretory protein is dried, impurities can be filtered through the filter box, so that the problem that subsequent biological functions and application effects of the dried secretory protein are affected due to the fact that the dried secretory protein contains cell debris or other impurities which are not removed is solved.

Description

Technical Field

[0001] This utility model relates to the field of Verticillium dahliae secretory protein technology, and in particular to a device for the production and collection of Verticillium dahliae secretory proteins. Background Technology

[0002] Verticillium dahliae is a typical soil-borne vascular pathogen, and its secreted proteins play a crucial role in the interaction between the pathogen and the host.

[0003] For example, CN203558995U discloses an apparatus for preparing Verticillium dahliae secreted proteins. This apparatus includes an ion exchange column, an ultrafilter, a desalting column, and a dryer. Specifically, in the upstream-to-downstream direction, a liquid pipeline with a valve is connected between the ion exchange column and the ultrafilter, between the ion exchange column and the desalting column, between the ultrafilter and the dryer, and between the desalting column and the dryer. This invention enables the high-yield and more complete production of Verticillium dahliae secreted proteins.

[0004] This patent can obtain Verticillium dahliae secreted protein with high yield and more completeness. However, existing devices do not filter the secreted protein after drying it. The dried secreted protein contains unremoved cell debris, culture medium components or other impurities, which may affect the purity and quality of the secreted protein, and thus affect its subsequent biological function and application effect.

[0005] Therefore, we propose a novel device for the production and collection of secreted proteins from Verticillium dahliae. Utility Model Content

[0006] The purpose of this invention is to solve the problem that existing devices do not filter the secreted protein of Verticillium dahliae after drying, resulting in the secreted protein containing unremoved cell debris, culture medium components, or other impurities. These impurities may affect the purity and quality of the secreted protein, thereby affecting its subsequent biological functions and application effects.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: a device for the production and collection of secreted proteins by Verticillium dahliae, comprising an operating table, a dryer mounted on the top of the operating table, a filter assembly mounted directly below the dryer on the operating table, the filter assembly comprising a filter box, a handle connected to the front surface of the filter box, slots formed on both outer surfaces of the handle, a fixing box connected to the surface of the operating table near the handle, a bidirectional screw inserted into the inside of the fixing box, bearings and handles connected to both ends of the bidirectional screw, movable blocks connected to both sides of the bidirectional screw, threaded holes formed at the bottom of both sets of movable blocks, and inserts connected to the inner sides of both sets of movable blocks.

[0008] Furthermore, the filter box and the operating table are connected in a sliding manner, and the size of the insert block matches the size of the slot.

[0009] Furthermore, the slot and the plug form a mating connection, and the bidirectional screw and the bearing form a rotating connection.

[0010] Furthermore, the movable block is connected to the bidirectional screw via a threaded hole.

[0011] Furthermore, the device includes a stirring assembly comprising a stirring tank, a stirring motor connected to the top of the stirring tank, a stirring rod connected to the output end of the stirring motor, slots being formed inside both sides of the stirring rod, springs being connected inside the slots, a push plate being connected to one side of the spring, a push rod being connected to one side of the push plate, and a scraper being connected to the outer surface of the push rod.

[0012] Furthermore, a valve is connected to the bottom of one side of the mixing tank, and a discharge pipe is connected to the surface of the valve.

[0013] Furthermore, a sedimentation tank is connected to the lower end of the feed pipe, and an elastic structure is formed between the spring and the push plate.

[0014] Compared with the prior art, the advantages and positive effects of this utility model are as follows:

[0015] 1. In this invention, after the secreted protein of Verticillium dahliae is dried, it will be filtered through a filter box to remove impurities, so as to prevent the dried secreted protein from containing unremoved cell fragments or other impurities, which would affect its subsequent biological functions and application effects.

[0016] 2. In this invention, after filtering the secretory protein of Verticillium dahliae, it can be stirred by a stirring rod. The stirring rod can effectively mix the filtered solution, ensuring that the secretory protein is evenly distributed in the solution and avoiding local concentrations that are too high or too low. Attached Figure Description

[0017] Figure 1 A three-dimensional structural diagram of a device for the production and collection of secreted proteins by Verticillium dahliae is provided for this utility model.

[0018] Figure 2 This invention presents a schematic diagram from another angle of the structure of a device for the production and collection of secreted proteins by Verticillium dahliae.

[0019] Figure 3 A cross-sectional structural diagram of a device for the production and collection of secreted proteins by Verticillium dahliae is provided for this utility model.

[0020] Figure 4 A partial exploded structural diagram of a device for the production and collection of secreted proteins by Verticillium dahliae, as proposed in this utility model;

[0021] Figure 5 A schematic diagram of the cross-sectional structure of the mixing tank of the device for the production and collection of secreted proteins of Verticillium dahliae proposed in this utility model;

[0022] Figure 6 for Figure 5 Enlarged structural diagram at point A in the middle.

[0023] Legend: 1. Control panel; 2. Dryer; 3. Filter assembly; 301. Filter box; 302. Handle; 303. Slot; 304. Fixing box; 305. Double-acting screw; 306. Bearing; 307. Handle; 308. Moving block; 309. Threaded hole; 310. Insert block; 4. Mixing assembly; 401. Mixing tank; 402. Mixing motor; 403. Mixing rod; 404. Slot; 405. Spring; 406. Push plate; 407. Push rod; 408. Scraper; 409. Valve; 410. Discharge pipe; 5. Sedimentation tank. Detailed Implementation

[0024] To better understand the above-mentioned objectives, features, and advantages of this utility model, the present utility model will be further described below with reference to the accompanying drawings and embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0025] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the present invention is not limited to the specific embodiments disclosed in the following specification.

[0026] Example 1, as Figure 1 - Figure 4As shown, this utility model provides a device for the production and collection of secreted proteins by Verticillium dahliae, including an operating table 1. A dryer 2 is installed on the top of the operating table 1. A filter assembly 3 is installed directly below the dryer 2 on the operating table 1. The filter assembly 3 includes a filter box 301. A handle 302 is connected to the front surface of the filter box 301. Slots 303 are provided on both outer surfaces of the handle 302. A fixing box 304 is connected to the surface of the operating table 1 near the handle 302. A bidirectional screw 305 is inserted into the fixing box 304. Shafts are connected to both ends of the bidirectional screw 305. The bearing 306 and handle 307 are connected to the two sides of the double-acting screw 305, and the two sets of moving blocks 308 are connected to the two sides of the handle 307. The bottom of the two sets of moving blocks 308 is provided with threaded holes 309, and the inner side of the two sets of moving blocks 308 is connected to the insert block 310. The filter box 301 and the operating table 1 are connected in a sliding connection. The size of the insert block 310 matches the size of the slot 303. The slot 303 and the insert block 310 are connected in a mating connection. The double-acting screw 305 and the bearing 306 are connected in a rotating connection. The moving block 308 is connected to the double-acting screw 305 through the threaded hole 309.

[0027] The overall effect of Embodiment 1 is that after the Verticillium dahliae secretes protein, it filters impurities through the filter box 301 after drying. When it is necessary to clean the impurities in the filter box 301 after long-term use, the handle 307 can be rotated in the reverse direction to drive the bidirectional screw 305 to rotate in the reverse direction. This allows the bidirectional screw 305 to rotate in the reverse direction simultaneously with the two sets of moving blocks 308 through the threaded hole 309. This, in turn, drives the two sets of moving blocks 308 to move to both sides simultaneously through the reverse threaded rotation, and drives the insert block 310 from the slot 3. Pull out the filter box 301 by pulling it outwards from handle 302. Then clean the filter box 301 to remove impurities. After cleaning, insert the filter box 301 back in for filtration. Rotate handle 307 forward to drive the bidirectional screw 305 to rotate forward, causing the two sets of insert blocks 310 to move inwards simultaneously and be inserted into the slot 303 for fixation. This prevents the dried secretory protein from containing unremoved cell debris or other impurities, which could affect its subsequent biological function and application effect.

[0028] Example 2, as Figure 5 and Figure 6As shown, the assembly includes a stirring component 4, which includes a stirring tank 401. A stirring motor 402 is connected to the top of the stirring tank 401. A stirring rod 403 is connected to the output end of the stirring motor 402. Slots 404 are provided on both sides of the stirring rod 403. A spring 405 is connected inside the slots 404. A push plate 406 is connected to one side of the spring 405. A push rod 407 is connected to one side of the push plate 406. A scraper 408 is connected to the outer surface of the push rod 407. A valve 409 is connected to the bottom of one side of the stirring tank 401. A discharge pipe 410 is connected to the surface of the valve 409. A sedimentation tank 5 is connected to the lower end of the discharge pipe 410. An elastic structure is formed between the spring 405 and the push plate 406.

[0029] The overall effect of Embodiment 2 is as follows: after filtering the secreted protein of Verticillium dahliae, the stirring motor 402 can be started to stir it through the stirring rod 403. The stirring rod 403 can effectively mix the filtered solution. At the same time, the spring 405 will also push the push plate 406 through its own elasticity, which will drive the push rod 407 to push the scraper 408 outward. This will cause the stirring rod 403 to stir while the scraper 408 cleans the inner wall of the mixing tank 401, improving the cleanliness of the mixing tank 401. This ensures that the secreted protein is evenly distributed in the solution and avoids local concentrations that are too high or too low. The stirred secreted protein of Verticillium dahliae will enter the sedimentation tank 5 through the feed pipe 410 for sedimentation. The valve 409 can control the opening and closing of the feed pipe 410. After sedimentation in the sedimentation tank 5, the sedimentation tank 5 can be withdrawn for the next step of operation.

[0030] Working principle: After the Verticillium dahliae secreted protein is dried, it will pass through the filter box 301 to filter impurities, so as to avoid the presence of unremoved cell debris or other impurities in the dried secreted protein, which would affect its subsequent biological functions and application effects. After filtering the Verticillium dahliae secreted protein, it can be stirred by the stirring rod 403. The stirring rod 403 can effectively mix the filtered solution, ensuring that the secreted protein is evenly distributed in the solution and avoiding local concentrations that are too high or too low.

[0031] The above are merely preferred embodiments of this utility model and are not intended to limit the utility model in any other way. Any person skilled in the art may make changes or modifications to the above-disclosed technical content to create equivalent embodiments for application in other fields. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of this utility model without departing from the technical solution of this utility model shall still fall within the protection scope of this utility model.

Claims

1. A device for producing secreted protein from Verticillium dahliae, characterized in that: Includes an operating table (1), on the top of the operating table (1) is a dryer (2), and a filter assembly (3) is located directly below the dryer (2) on the operating table (1); The filter assembly (3) includes a filter box (301), a handle (302) is connected to the front surface of the filter box (301), and slots (303) are provided on both outer surfaces of the handle (302). A fixed box (304) is connected to the surface of the operating table (1) near the handle (302). A bidirectional screw (305) is inserted into the inside of the fixed box (304). A bearing (306) and a handle (307) are respectively connected to both ends of the bidirectional screw (305). Movable blocks (308) are connected to both sides of the bidirectional screw (305). Threaded holes (309) are provided at the bottom of both sets of movable blocks (308). Insert blocks (310) are connected to the inner side of both sets of movable blocks (308).

2. The device for producing secreted protein from *Verticillium dahliae* according to claim 1, characterized in that: The filter box (301) and the operating table (1) are connected in a sliding manner, and the size of the insert (310) matches the size of the slot (303).

3. The device for producing secreted protein from *Verticillium dahliae* according to claim 2, characterized in that: The slot (303) and the plug (310) form a mating connection, and the bidirectional screw (305) and the bearing (306) form a rotating connection.

4. The device for producing secreted protein from *Verticillium dahliae* according to claim 3, characterized in that: The movable block (308) is threadedly connected to the bidirectional screw (305) through a threaded hole (309).

5. A collection device, including a stirring assembly (4), characterized in that: The stirring assembly (4) includes a stirring tank (401); A stirring motor (402) is connected to the top of the mixing tank (401). A stirring rod (403) is connected to the output end of the stirring motor (402). A slot (404) is opened inside both sides of the stirring rod (403). A spring (405) is connected inside the slot (404). A push plate (406) is connected to one side of the spring (405). A push rod (407) is connected to one side of the push plate (406). A scraper (408) is connected to the outer surface of the push rod (407).

6. The collection device according to claim 5, characterized in that: A valve (409) is connected to the bottom of one side of the mixing tank (401), and a feed pipe (410) is connected to the surface of the valve (409).

7. The collection device according to claim 6, characterized in that: The lower end of the feed pipe (410) is connected to a sedimentation tank (5), and the spring (405) and the push plate (406) form an elastic structure.

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