Chip card box for evaluating interaction of microorganisms and materials

Abstract

A plurality of liquid storage bags and waste liquid bags are arranged in a liquid storage area, a liquid separation chip and a plurality of peristaltic pumps and electromagnetic valves are arranged in a liquid separation area, four reaction chips are arranged in a reaction area, each reaction chip can support two parallel experiments, and the different areas are connected through hoses. A culture solution in the liquid storage area is controlled by a pump valve and a liquid separation chip in the liquid separation area, flows into different reaction chips, is subjected to microbial culture and interaction experiments with materials, and finally flows into a waste liquid bag. The reaction chip can be used for collecting electrochemical signals, and real-time observation can be carried out through a microscope. The chip card box provided by the utility model is designed to be totally closed, is high in integration level, can support simultaneous operation of a plurality of reactions, and is safe and reliable.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of biomedical detection, concretely relates to a chip card box of evaluating microorganism and material interaction. BACKGROUND

[0002] Different environmental factors make the behavior performance and survival strategy of microorganisms possibly different. The prior art starts from short-term culture of microorganisms, and focuses on exploring the microorganism species, content and threat to personnel, and less researches the device corrosion and other conditions caused by long-term growth of microorganisms. Therefore, it is urgent to establish a system for monitoring and evaluating the pollution and damage of microorganisms to various materials, and to explore the film forming of microorganisms under different environmental conditions and the mechanism of the action of microorganisms on materials, so that effective microorganism pollution protection strategies can be established, and long-term use of various structural materials can be ensured. CONTENT

[0003] In order to overcome the problems in the prior art, the utility model provides a chip card box of evaluating microorganism and material interaction, which can culture microorganisms and study the interaction between microorganisms and materials.

[0004] The utility model provides the following technical scheme:

[0005] A chip card box of evaluating microorganism and material interaction, comprising a liquid storage bag, a waste liquid bag, a liquid distribution chip and a plurality of reaction chips sealed in a box body, wherein the reaction chips are provided with a plurality of microorganism culture and monitoring cavities, the liquid distribution chip is provided with a peristaltic pump and a solenoid valve, the liquid inlet passage of each reaction chip is connected to the liquid storage bag through the solenoid valve and the peristaltic pump of the liquid distribution chip, and the liquid outlet passage of each reaction chip is connected to the waste liquid bag through the liquid distribution chip.

[0006] Further, an optical detection window is arranged at a position corresponding to the microorganism culture and monitoring cavity at the bottom of the card box.

[0007] Further, the reaction chip is provided with an electric heating sheet at the top of the microorganism culture and monitoring cavity, and a temperature sensor is arranged on the inner surface of the cavity of the microorganism culture and monitoring cavity.

[0008] Further, the liquid distribution chip is provided with a first liquid distribution chip and a second liquid distribution chip, the peristaltic pump is connected to the liquid path of the liquid storage bag and the first liquid distribution chip, the first liquid distribution chip divides the liquid inlet passage into a plurality of branch liquid inlet passages, the branch liquid inlet passages are in communication with the reaction chips, the solenoid valve controls the opening and closing of the branch liquid inlet passages, and the second liquid distribution chip is connected to the liquid outlet passage of the reaction chip and the liquid storage bag.

[0009] Further, the reaction chip is connected with the first liquid separation chip and the second liquid separation chip through quick connectors.

[0010] Further, the several chip card boxes can be connected in stack, and the first electric connector, the second electric connector and the third electric connector are arranged on the card box body, the first electric connector is externally connected with a control power supply, and half of the pins are connected with the second electric connector, and the other half of the pins are connected with a peristaltic pump electromagnetic valve circuit and the third electric connector.

[0011] The second electric connector is externally connected with the stacked chip card boxes, and the third electric connector is arranged on the reaction chip shell, the male head is connected with the first electric connector, and the female head is connected with the detection electrode chip, the electric heating sheet and the temperature sensor.

[0012] By adopting the technical scheme, the chip card box has the following beneficial effects:

[0013] (1) The chip card box can complete microbial culture and interaction research in a closed liquid path, and is safe and reliable.

[0014] (2) The card box has high integration degree, and can complete experiments automatically through electric control, without manual operation.

[0015] (3) The chip card box has multiple experimental groups, and can simultaneously carry out multiple parallel experiments.

[0016] (4) The chip card box has multiple electric connectors, and can stack multiple card boxes to simultaneously carry out experiments, thereby improving experimental flux. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 is a structural schematic view of the chip card box for evaluating the interaction between microorganisms and materials;

[0018] Figure 2 is a structural schematic view of a photographing observation window of the chip card box for evaluating the interaction between microorganisms and materials;

[0019] Figure 3 is a structural schematic view of a reaction chip in the chip card box;

[0020] Figure 4 is a structural schematic view of a reaction chamber of the reaction chip in the chip card box;

[0021] Wherein: 1-Liquid storage area, 4-Liquid storage bag, 5-Waste liquid bag, 2-Dispensing area, 6-Peristaltic pump, 7-Solenoid valve, 8-First dispensing chip, 9-Second dispensing chip, 3-Reaction area, 10-Reaction chip, 11-First electrical connector, 12-Second electrical connector, 13-Third electrical connector, 14-Optical detection window, 15-Electrical heating element, 16-Detection electrode chip, 17-Temperature sensor. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the structural diagrams and specific embodiments described herein are only for explaining the present utility model and are not intended to limit the present utility model.

[0023] Example 1

[0024] like Figure 1 , Figure 2 As shown, this utility model provides a chip cartridge for evaluating the interaction between microorganisms and materials, including a storage area 1, a dispensing area 2, and a reaction area 3 sealed within the chip cartridge. The storage area contains a storage bag 4 and a waste bag 5, serving to store culture medium and waste liquid. The dispensing area is equipped with a dispensing chip, which includes a peristaltic pump 6 and a solenoid valve 7. The inlet passage of each reaction chip is connected to the storage bag via the solenoid valve and peristaltic pump of the dispensing chip, and the outlet passage of each reaction chip is connected to the waste bag via the dispensing chip, thus distributing the culture medium to each reaction chip and collecting the waste liquid from each reaction chip into the waste bag. The storage bag stores bacterial culture medium. The reaction area contains four reaction chips 10 for microbial culture and corrosion research experiments, and also includes a first electrical connector 11, a second electrical connector 12, and a third electrical connector 13 to supply power to each structure.

[0025] The reaction chip 10 has two microbial culture and monitoring chambers. The microbial culture and monitoring chambers contain lyophilized microbial powder and detection electrode chip 16. The surface of the detection electrode chip is coated with a material to be etched.

[0026] The liquid dispensing chip consists of a first dispensing chip 8 and a second dispensing chip 9. A peristaltic pump is connected to the liquid path of the storage bag and the first dispensing chip. The first dispensing chip divides the inlet channel into several branch inlet channels, which can communicate with the microbial culture and monitoring chambers. Solenoid valves control the opening and closing of the branch inlet channels. The second dispensing chip 9 connects the outlet path of the reaction chip and the storage bag. The culture medium in the storage bag 4 flows into the first dispensing chip 8 under the action of the peristaltic pump. Under the action of the dispensing chip, the culture medium is divided into 8 channels, and the 8 solenoid valves are opened and closed sequentially. The culture medium enters the 8 microbial culture and monitoring chambers in the 4 reaction chips in sequence, completing the dispensing of culture medium for the bacterial powder.

[0027] The top of the microorganism culture and monitoring chamber of the reaction chip is provided with an electric heating sheet 15, and the inner surface of the microorganism culture and monitoring chamber is provided with a temperature sensor 17. After the pump valve is closed, the electric heating sheet is powered on, and the temperature is controlled through the temperature sensor. The temperature in the chamber of the microorganism culture and monitoring chamber is controlled at a temperature suitable for the growth of bacteria, and culture and corrosion experiments are carried out. After the experiment, the peristaltic pump is opened to continue to pass the culture medium, and the culture medium in the chamber of the microorganism culture and monitoring chamber is pushed into the waste liquid bag through the second liquid distribution chip.

[0028] An optical detection window 14 is arranged at the bottom of the card box and corresponds to the position of the microorganism culture and monitoring chamber. During the experiment, real-time observation and recording of experimental results can be realized by taking pictures through the optical detection window at the bottom of the card box under a microscope.

[0029] Each reaction chip can support two parallel experiments, and different regions are connected by a hose. The culture solution in the liquid storage area is controlled by the pump valve and the liquid distribution chip in the liquid distribution area, flows into different reaction chips, and finally flows into the waste liquid bag. The reaction chip can collect electrochemical signals and can be observed in real time through a microscope.

[0030] Example 2

[0031] As shown in Figure 3 , Figure 4 , the reaction chip includes a reaction chip shell, a microorganism culture and monitoring chamber, an electric heating sheet 15, a detection electrode chip 16, and a temperature sensor 17. The inlet and outlet of the reaction chamber are connected to the external liquid circuit by a 2-way straight connector and a hose. The detection electrode chip, the electric heating sheet, and the temperature sensor are connected to a third electric connector for power supply and electrochemical signal collection. On the surface of the detection electrode chip, a layer of corrosion-resistant material to be studied is plated.

[0032] The freeze-dried bacterial powder is placed in the microorganism culture and monitoring chamber in advance. When the culture medium is introduced from the inlet of the microorganism culture and monitoring chamber, power is supplied by the third electric connector, heated by the electric heating sheet, and the temperature is continuously fed back to the control center by the temperature sensor for adjustment. The temperature is controlled at the optimum temperature for bacterial growth, and the bacteria are cultured. During this process, appropriate amount of culture medium can be supplemented in time according to the growth state of the bacteria to supply the growth of the bacteria. During the growth of the bacteria, the detection electrode chip can continuously transmit electrochemical signals to the external signal collection equipment for corrosion research of the bacteria on the material.

[0033] The reaction chip is connected to the first liquid distribution chip and the second liquid distribution chip through a quick connector. The quick connector is a bidirectional sealed break connector, which can safely and quickly disassemble the reaction chip without the risk of liquid leakage, and avoids the overflow of microorganisms in the flow path to the external environment during disassembly.

[0034] The plurality of chip card boxes can be connected in stack, and the plurality of chip card boxes can be detected simultaneously. The first electric connector 11, the second electric connector 12 and the third electric connector 13 are arranged on the card box box body, the first electric connector is externally connected with a control power supply, and internally connected with the second electric connector through half of the pins, and connected with the peristaltic pump electromagnetic valve circuit and the third electric connector through the other half of the pins. The first electric connector is a unified circuit interface of the card box, and supplies power to the card box as a whole. The second electric connector is externally connected with the stacked chip card boxes, and supplies power to the stacked chip card boxes, so that the purpose of simultaneous experiment of the plurality of card boxes can be achieved, and the experiment flux is improved. The third electric connector is arranged on the reaction chip shell, and the male head is connected with the first electric connector, and the female head is connected with the detection electrode chip, the electric heating sheet and the temperature sensor. The third electric connector is integrated with the reaction chip, so that the reaction chip can be conveniently disassembled and used at any time.

[0035] The above-mentioned embodiments only express the implementation manners of the present application, and the description is relatively specific and detailed, but it should not be understood as the limitation of the patent scope of the present application. It should be pointed out that, for ordinary skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which all belong to the protection scope of the present application. Therefore, the protection scope of the present application patent should be subject to the appended claims.

Claims

1. A chip card cassette for evaluating the interaction of microorganisms with materials, comprising a reservoir bag, a waste bag, a distribution chip and a plurality of reaction chips sealed in a cassette body, characterized in that The reaction chip is provided with a plurality of microbial culture and monitoring chambers, the distribution chip is provided with a peristaltic pump and a solenoid valve, the liquid inlet passage of each reaction chip is connected to a liquid storage bag through the solenoid valve and the peristaltic pump of the distribution chip, the liquid outlet passage of each reaction chip is connected to a waste liquid bag through the distribution chip, the liquid storage bag stores a bacterial culture medium, the microbial culture and monitoring chambers store microbial freeze-dried powder and a detection electrode chip, and the surface of the detection electrode chip is plated with a material to be corroded.

2. The card box of claim 1, wherein An optical detection window is arranged at the bottom of the card box and the corresponding position of the microbial culture and monitoring chamber.

3. The card box of claim 2, wherein, The reaction chip is provided with an electric heating sheet at the top of the microbial culture and monitoring chamber, and the inner surface of the chamber of the microbial culture and monitoring chamber is provided with a temperature sensor.

4. The card kit of claim 3, wherein, The distribution chip is provided with a first distribution chip and a second distribution chip, the peristaltic pump is connected to the liquid path of the liquid storage bag and the first distribution chip, the first distribution chip divides the liquid inlet passage into a plurality of branch liquid inlet passages, the branch liquid inlet passages can communicate with the reaction chip, the solenoid valve controls the opening and closing of the branch liquid inlet passages, and the second distribution chip is connected to the liquid outlet passage of the reaction chip and the liquid storage bag.

5. The card kit of claim 1, wherein, The reaction chip is connected to the first distribution chip and the second distribution chip through a quick plug.

6. The card box of claim 1, wherein, A plurality of chip card boxes can be connected in stack, a first electric connector, a second electric connector and a third electric connector are arranged on the card box body, the first electric connector is externally connected to a control power supply, internally connected to the second electric connector, and connected to the peristaltic pump solenoid valve circuit and the third electric connector; The second electric connector is externally connected to the stacked chip card boxes; the third electric connector is arranged on the reaction chip shell, the male head is connected to the first electric connector, and the female head is connected to the detection electrode chip, the electric heating sheet and the temperature sensor.

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