Novel 40-layer cell factory with metal net interlayer

Abstract

The utility model relates to the technical field of cell culture in biopharmaceutical production, in particular to a novel 40-layer cell factory with a metal net interlayer, which is characterized in that the metal net interlayer is designed in a partition plate between culture layers of the original 40-layer cell factory and comprises a metal net structure and an internal temperature sensor component; and a T-shaped all-metal sheet covered edge is arranged on the peripheral edge of the layer of the metal net-shaped structure. According to the novel design structure, uniform temperature rise of each cell culture layer can be realized, the temperature rise efficiency is high, temperature values at different positions can be conveniently monitored, and the stability and controllability of biological products and vaccine products taking a 40-layer cell factory as a cell or virus culture container in the research and development, pilot plant test and industrial production processes are greatly improved.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology in biopharmaceutical vaccine production, and in particular to a novel 40-layer cell factory with a metal mesh interlayer. The feature is that a metal mesh interlayer is designed in the partition between each culture layer of the original 40-layer cell factory, which includes a metal mesh structure and an internal temperature sensor component. The edges of the layer containing the metal mesh structure are surrounded by T-shaped all-metal sheets. Background Technology

[0002] Currently, in the biopharmaceutical industry, especially in the vaccine industry, when using 40-layer cell factories for research, pilot-scale, or industrial-scale cell culture, the required number of cell suspensions are generally evenly placed within each cell culture layer of the 40-layer cell factory (the cell suspension temperature is generally ≤ room temperature 25℃), and then placed in a greenhouse or incubator with suitable culture conditions to begin cell culture. Cell or virus culture temperatures are generally in the range of 32℃~37℃, both higher than the initial culture medium temperature. Therefore, a heating process is required during culture, and the duration of this heating is crucial to process stability and the target product. Existing cell factory products on the market are generally made of polystyrene, with a thermal conductivity of 0.08W / (m·K). Its low thermal conductivity during heating results in a relatively slow heating process. A 40-layer cell factory is large, equivalent to four 10-layer cell factories stacked together, resulting in poor heating uniformity and low heating efficiency during culture.

[0003] Secondly, since both cell culture and virus growth stages have very high temperature requirements, temperature verification is generally required during product development, pilot production, or commercial production. However, in practice, only the temperature of the culture environment or equipment can be verified, making it difficult to verify the temperature within each culture layer of a multi-layered cell factory.

[0004] Verifying the temperature uniformity of the culture medium in each layer of a 40-layer cell factory is a challenge in the industry. It is difficult to verify or confirm this without damaging or wasting the factory's consumables.

[0005] The aforementioned defects significantly impact product research and development, pilot testing, and production results. Therefore, it is necessary to provide a novel 40-layer cell factory to address these technical problems. Utility Model Content

[0006] The technical problem to be solved by this invention is to provide a novel 40-layer cell factory that addresses the shortcomings of existing technologies.

[0007] To achieve the above objectives, the technical solution of this utility model is as follows: a metal mesh interlayer is designed in the partition between each culture layer of the existing 40-layer cell factory. This metal mesh interlayer is a structure with uniform heating and contains a design component for monitoring the internal temperature of the container. Specifically, a metal mesh interlayer 1 (see [reference]) is designed and placed in the partition at the bottom of the cell growth space of any layer from 1 to 40 layers in the cell factory. Figure 1 It includes a metal mesh structure 2 and internal temperature sensor assemblies 4, 5, and 6 (see...). Figure 2 , 3 4). The metal mesh structure 2 extends to the perimeter of the partition, forming a T-shaped metal sheet edging 3 (see Figure 2 , 3 The metal mesh structure 2 facilitates better heat conduction from the incubator to the metal mesh layer 3. The height of the T-shaped metal sheet edging 3 is basically the same as the thickness of the partition. The metal mesh interlayer 1 uses aluminum or silver, which have high thermal conductivity, good ductility, and moderate material cost, with a thickness of 0.2-0.5 mm. Among them, aluminum has a thermal conductivity of 237 W / (m·K); silver has a thermal conductivity of 429 W / (m·K), while the thermal conductivity of existing polystyrene materials for cell factories is only 0.08 W / (m·K). The higher the thermal conductivity, the faster the cell factory heats up.

[0008] Furthermore, in this novel 40-layer cell factory, a metal mesh interlayer 1 is preferably placed every 5 to 10 layers. The metal mesh structure 2 is embedded inside a single-layer partition between two layers of the cell factory. The metal mesh structure 2 contains a thin-film thermocouple 4 with a thickness of 0.2 to 0.5 mm. Each thermocouple 4 has a signal line extending to a T-shaped metal sheet 3 on its outer surface. The ends of the signal lines have strongly magnetic but different magnetic pole metal planar dots 5 and 6, facilitating quick connection between the temperature signal line and temperature monitoring equipment. The magnetic contact design of the magnetic contacts 5 and 6 has a smooth, non-protruding surface, suitable for use in 40-layer cell factories, such as horizontal laying, shaking, or intelligent robotic arm gripping scenarios.

[0009] The distribution of the magnetic metal planar dots 5 and 6 at the end of the signal line can be designed at any position around the T-shaped metal sheet edging 3; multiple sensors can be designed and arranged at any point on the internal metal mesh structure 2 for the S thermocouple sheet 4, and the signal line is routed through a metal mesh cutout design to avoid insulation.

[0010] Compared with existing products on the market, this invention increases the unit cost due to the addition of a metal mesh structure and temperature monitoring components. However, the prices of the materials used, such as aluminum and silver, are relatively controllable, and because metals have excellent ductility, their usage in each cell factory is very low. The temperature control components are also currently very mature, resulting in a lower overall cost. Considering the beneficial technical effects of this invention, the cost increase is essentially negligible. Attached Figure Description

[0011] Figure 1 This is a schematic diagram of the 40-layer cell factory and metal mesh interlayer of this utility model;

[0012] Figure 2 This is a top view of the 40-layer cell factory metal mesh interlayer and temperature sensor assembly of this utility model;

[0013] Figure 3 This is a side view and a side perspective view of the outer side of the 40-layer cell factory metal mesh interlayer of this utility model;

[0014] Figure 4 This is a top view of the 40-layer cell factory temperature sensor assembly of this utility model.

[0015] The numbers labeled on the image have the following meanings: 1 represents the location of the metal mesh interlayer distributed in the 40-layer cell factory; 2 represents the metal mesh structure in the metal mesh interlayer; 3 represents the T-shaped metal sheet edging on the outside of the metal mesh interlayer; 4 represents the S-thermocouple sheet in the metal mesh structure; 5 and 6 represent the + / - magnetic planar metal dots (with insulating ring components around the dots) of the temperature sensor signal line terminals on the T-shaped metal edging. Detailed Implementation

[0016] To facilitate understanding of this utility model, a more comprehensive description will be provided below with reference to the accompanying drawings. The drawings show typical embodiments of this utility model.

[0017] Example 1:

[0018] like Figure 1 As shown, a novel 40-layer cell factory containing a metal mesh interlayer 1 has a structure including a metal mesh structure 2, T-shaped metal edging 3 on the four sides, and a novel design of temperature sensor components 4, 5, and 6.

[0019] During cell or virus culture, after the suspension is evenly placed in the cell factory, it is placed in a constant temperature incubator or constant temperature chamber and the temperature is gradually increased to the range of 32-37℃. Because metals have a higher thermal conductivity than plastic materials such as polystyrene (aluminum: 237 W / (m·K); silver: 429 W / (m·K); polystyrene: 0.08 W / (m·K)), component 3 rapidly conducts heat to component 2 during the heating process in the incubator. Compared to cell factories made solely of styrene, the internal heating is faster and more uniform, achieving the same heating efficiency as a 5-10 layer cell factory. This compensates for the slow internal heating rate and uneven temperature distribution caused by a large number of layers in the cell factory, thus ensuring uniform growth of the cultured cells or viruses at all levels and allowing for better process quality control.

[0020] Example 2:

[0021] The adoption of this novel 40-layer cell factory achieves both reliability and convenience in verifying temperature uniformity and heating efficiency. First, the cell suspension (generally with a liquid temperature ≤25℃) is pumped into the cell factory and placed in the appropriate culture environment. Then, the temperature detection device is magnetically attached to the + / - magnetic plane metal dots 5 and 6 of the temperature sensor signal line terminals using wires with magnetic terminals. Since temperature sensor signal line terminals are located on both sides of the metal interlayer of the cell factory, wiring can be easily connected to any convenient location, facilitating operation. The temperature sensor S thermocouple 4 on the metal mesh structure 2 can transmit temperature signals in real time, recording the temperature of the middle culture layer of the 40-layer cell factory. This allows for temperature verification of the cultured cells or viruses, obtaining optimal production process parameters.

[0022] The temperature sensor and the strong magnetic dot on the metal plane at the end of the signal line designed in this utility model can be quickly connected to the temperature verification instrument. It does not occupy space, has no pollution source, meets the cleanliness requirements of pharmaceutical GMP, and solves the problem of internal temperature monitoring and verification in a 40-layer cell factory. It can monitor the temperature at any time and provide accurate parameter guarantee for the growth of cell viruses.

[0023] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A novel 40-layer cell factory with metal mesh interlayer, characterized in that The original 40 layers of cell factory each culture layer in the middle of the partition is designed with a metal mesh sandwich, which contains a metal mesh structure and an internal temperature sensor assembly. The metal mesh structure is surrounded by a T-shaped full metal sheet edge.

2. The novel 40-layer cell factory with metal mesh interlayer according to claim 1, characterized in that The material used for the preparation is aluminum or silver, which has high thermal conductivity, good ductility and moderate material cost, and the thickness is 0.2-0.5mm.

3. The novel 40-layer cell factory with metal mesh interlayer according to claim 1, characterized in that The metal mesh structure contains a sheet S thermocouple with a thickness of 0.2-0.5mm. The thermocouple signal line extends to the outside of the T-shaped full metal sheet edge at both ends of the mesh structure. The end of the signal line has a strong magnetic but different magnetic pole metal plane dot, which facilitates the quick connection of the temperature signal line and the temperature monitoring equipment.

4. The novel 40-layer cell factory with metal mesh interlayer according to claim 3, characterized in that The distribution position can be designed at any position around the T-shaped metal sheet edge.

5. The novel 40-layer cell factory with metal mesh interlayer according to claim 3, characterized in that Multiple sensors can be designed and arranged at any position on the internal metal mesh structure plane.

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