Ultrafiltration device for laboratory research

Abstract

The utility model discloses an ultrafiltration device for laboratory research, and relates to the technical field of cell preparation. The device comprises a heat preservation shell, wherein an ultrafiltration mechanism and a shunting mechanism are arranged on the heat preservation shell; the ultrafiltration mechanism comprises a reverse assembly, a limiting assembly and an ultrafiltration assembly, the reverse assembly comprises a motor fixedly connected to the inner wall of the heat preservation shell, the output end of the motor is fixedly connected with a rotating shaft, the rotating shaft is fixedly connected with a first bevel gear, the heat preservation shell is rotationally connected with a rotating cylinder, and the rotating cylinder is fixedly connected with a second bevel gear. According to the utility model, through the ultrafiltration mechanism, the driving motor drives the two rotating drums to rotate reversely through the first bevel gear, so that the rotating drums rotating reversely drive the inclined ultrafiltration membrane to stir a culture solution, and opposite counter-flow acting forces are formed in the culture solution, so that cells are promoted to quickly and frequently contact with the ultrafiltration membrane; compared with one-way rotation, filtering of the device is accelerated to a certain extent, and the practicability of the device is improved.

Description

Technical Field

[0001] This invention belongs to the field of cell preparation technology, and in particular relates to an ultrafiltration device for laboratory research. Background Technology

[0002] Cell preparation refers to the process of isolating, culturing, expanding, processing, and preserving cells from tissues or organs within an organism. Cell preparation technology is widely used in basic research, clinical medicine, drug screening, genetic engineering, vaccine production, tissue engineering, regenerative medicine, and many other fields. As an important foundational technology in modern biology, medicine, and biopharmaceuticals, cell preparation technology covers multiple aspects from cell isolation and culture to expansion and preservation. With technological advancements, cell preparation technology continues to develop, providing crucial support for cell therapy, gene therapy, drug screening, and vaccine production, and has broad application prospects.

[0003] However, some ultrafiltration devices used for cell separation and purification have relatively simple structures. They usually use a unidirectional rotating ultrafiltration membrane and use centrifugal force to promote the cells cultured in the incubator to contact the ultrafiltration membrane for purification and separation. The unidirectional rotating culture medium causes the cells to be subjected to flow force and directly contact the ultrafiltration membrane for filtration, which has a relatively small filtration force and makes filtration relatively slow to some extent. Utility Model Content

[0004] The purpose of this invention is to provide an ultrafiltration device for laboratory research. By setting up an ultrafiltration mechanism, a drive motor drives two rotating drums to rotate in opposite directions through a first bevel gear. The counter-rotating drums cause the inclined ultrafiltration membrane to stir the culture medium, forming a counter-current force in the culture medium. This promotes rapid and frequent contact between cells and the ultrafiltration membrane, solving the problem that the unidirectional rotation of the ultrafiltration membrane leads to relatively slow filtration.

[0005] To solve the above-mentioned technical problems, this utility model is achieved through the following technical solution:

[0006] This utility model is an ultrafiltration device for laboratory research, including an insulated shell, on which an ultrafiltration mechanism and a flow splitting mechanism are provided;

[0007] The ultrafiltration mechanism includes a reverse component, a limiting component, and an ultrafiltration component. The reverse component includes a motor fixedly connected to the inner wall of the heat insulation shell. The output end of the motor is fixedly connected to a rotating shaft. The left end of the rotating shaft is fixedly connected to a first bevel gear. The inner wall of the heat insulation shell is rotatably connected to two rotating cylinders. The outer walls of the two rotating cylinders are fixedly connected to second bevel gears, and the two second bevel gears mesh with the first bevel gear.

[0008] Furthermore, the limiting component includes two limiting grooves formed on the inner wall of the heat insulation shell, and the inner walls of the two limiting grooves are rotatably connected to limiting rings. The sides of the two limiting rings that are close to each other are fixedly connected to the sides of the two rotating cylinders that are far apart from each other.

[0009] Furthermore, the ultrafiltration assembly includes sealing grooves that are opened on the sides of the two rotating cylinders that are close to each other, the inner walls of the two sealing grooves are rotatably connected to each other, an ultrafiltration membrane is fixedly connected to the inner walls of the two rotating cylinders, and a fixed funnel is rotatably connected to the inner walls of the two rotating cylinders.

[0010] Furthermore, the ends of the two fixed funnels that are far apart from each other extend to the top and bottom surfaces of the insulation shell and are fixedly connected to the insulation shell. A high-pressure pump is fixedly connected to the bottom surface of the insulation shell. The top output end of the high-pressure pump is fixedly connected to the bottom end of the lower fixed funnel. A connecting pipe is fixedly connected to the front output end of the high-pressure pump.

[0011] Furthermore, the diversion mechanism includes a diversion component and an adjustment component. The diversion component includes a fixed plate fixedly connected to the front side of the insulation shell. A fixed cylinder is fixedly connected to the bottom surface of the fixed plate, and the outer wall of the fixed cylinder is fixedly connected to the front end of the connecting pipe.

[0012] Furthermore, two discharge pipes are fixedly connected to the outer wall of the fixed cylinder, and a diverter cylinder is rotatably connected to the inner wall of the fixed cylinder. Two connection holes are opened on the outer wall of the diverter cylinder.

[0013] Furthermore, the adjustment assembly includes a rotating handle fixedly connected to the top surface of the diverter cylinder. The top end of the rotating handle extends to the top surface of the fixed plate and is rotatably connected to the inner wall of the fixed plate. Several grooves are provided on the top surface of the fixed plate and the outer wall of the rotating handle.

[0014] This utility model has the following beneficial effects:

[0015] 1. By setting up an ultrafiltration mechanism, the drive motor drives two rotating drums to rotate in opposite directions through the first bevel gear. The counter-rotating drums cause the inclined ultrafiltration membrane to stir the culture medium, forming a counter-current force in the culture medium. This promotes rapid and frequent contact between cells and the ultrafiltration membrane. Compared with unidirectional rotation, this speeds up the filtration process to a certain extent and improves the practicality of the device.

[0016] 2. By setting up a diversion mechanism, the operator can selectively align the two connection holes with the connection pipe and the specific discharge pipe by turning the rotating handle, so as to separate and discharge the culture medium containing qualified cells and the waste liquid, which facilitates subsequent processing and further improves the practicality of the device.

[0017] Of course, any product implementing this utility model does not necessarily need to achieve all of the advantages described above at the same time. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0020] Figure 2 This is a schematic diagram of the rear cross-sectional structure of this utility model;

[0021] Figure 3 This is a schematic diagram of the left-side cross-sectional structure of this utility model;

[0022] Figure 4 for Figure 2 Enlarged structural diagram at point A;

[0023] Figure 5 for Figure 3 A magnified structural diagram at point B in the middle.

[0024] The attached diagram lists the components represented by each number as follows:

[0025] 1. Insulated outer shell; 2. Ultrafiltration mechanism; 3. Diverting mechanism; 21. Motor; 22. Rotating shaft; 23. First bevel gear; 24. Rotating drum; 25. Second bevel gear; 26. Limiting groove; 27. Limiting ring; 28. Sealing groove; 29. ​​Ultrafiltration membrane; 210. Fixed funnel; 211. High-pressure pump; 212. Connecting pipe; 31. Fixed plate; 32. Fixed cylinder; 33. Discharge pipe; 34. Diverting cylinder; 35. Connecting hole; 36. Rotating handle; 37. Groove. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0027] Please see Figure 1-5 As shown, this utility model is an ultrafiltration device for laboratory research, including an insulated shell 1, on which an ultrafiltration mechanism 2 and a flow splitting mechanism 3 are provided;

[0028] The ultrafiltration mechanism 2 includes a reverse assembly, a limiting assembly, and an ultrafiltration assembly. The reverse assembly includes a motor 21 fixedly connected to the inner wall of the heat insulation shell 1. The output end of the motor 21 is fixedly connected to a rotating shaft 22. The left end of the rotating shaft 22 is fixedly connected to a first bevel gear 23. The inner wall of the heat insulation shell 1 is rotatably connected to two rotating cylinders 24. The outer walls of the two rotating cylinders 24 are fixedly connected to second bevel gears 25. The two second bevel gears 25 mesh with the first bevel gear 23.

[0029] Among them, such as Figure 2 , Figure 3 and Figure 4 As shown, the limiting assembly includes two limiting grooves 26 formed on the inner wall of the insulation shell 1. The inner walls of the two limiting grooves 26 are rotatably connected to limiting rings 27. The sides of the two limiting rings 27 that are close to each other are fixedly connected to the sides of the two rotating cylinders 24 that are far apart from each other. The ultrafiltration assembly includes sealing grooves 28 formed on the sides of the two rotating cylinders 24 that are close to each other. The inner walls of the two sealing grooves 28 are rotatably connected to each other. The inner walls of the two rotating cylinders 24 are fixedly connected to ultrafiltration membranes 29. The inner walls of the two rotating cylinders 24 are rotatably connected to fixed funnels 210. The ends of the two fixed funnels 210 that are far apart from each other extend to the top and bottom surfaces of the insulation shell 1 and are fixedly connected to the insulation shell 1. The bottom surface of the insulation shell 1 is fixedly connected to a high-pressure pump 211. The top output end of the high-pressure pump 211 is fixedly connected to the bottom end of the fixed funnel 210 located on the lower side. The front output end of the high-pressure pump 211 is fixedly connected to a connecting pipe 212.

[0030] By setting up the ultrafiltration mechanism 2, the drive motor 21 drives the two rotating drums 24 to rotate in opposite directions through the first bevel gear 23. The rotating drums 24 rotate in opposite directions, causing the inclined ultrafiltration membrane 29 to stir the culture medium. This creates opposing flow forces in the culture medium, promoting rapid and frequent contact between cells and the ultrafiltration membrane 29. Compared with unidirectional rotation, this speeds up the filtration process to a certain extent and improves the practicality of the device.

[0031] Among them, such as Figure 3 and Figure 5 As shown, the diversion mechanism 3 includes a diversion component and an adjustment component. The diversion component includes a fixed plate 31 fixedly connected to the front side of the insulation shell 1. A fixed cylinder 32 is fixedly connected to the bottom surface of the fixed plate 31. The outer wall of the fixed cylinder 32 is fixedly connected to the front end of the connecting pipe 212. Two discharge pipes 33 are fixedly connected to the outer wall of the fixed cylinder 32. A diversion cylinder 34 is rotatably connected to the inner wall of the fixed cylinder 32. Two connection holes 35 are opened on the outer wall of the diversion cylinder 34. The adjustment component includes a rotating handle 36 fixedly connected to the top surface of the diversion cylinder 34. The top end of the rotating handle 36 extends to the top surface of the fixed plate 31 and is rotatably connected to the inner wall of the fixed plate 31. Several grooves 37 are opened on the top surface of the fixed plate 31 and the outer wall of the rotating handle 36.

[0032] By setting up the diversion mechanism 3, the staff can selectively align the two connection holes 35 with the connection tube 212 and the specific discharge tube 33 by rotating the rotary handle 36 to drive the diversion tube 34, so as to separately discharge the culture medium with qualified cells and the waste liquid, which facilitates subsequent processing and further improves the practicality of the device.

[0033] A specific application of this embodiment is as follows: By setting up the ultrafiltration mechanism 2, the operator fixes the culture medium outlet pipe in the carbon dioxide incubator for culturing cells to the top of the upper fixed funnel. The culture medium containing cells is drawn through the upper fixed funnel 210 into the upper rotating drum 24 to contact the upper ultrafiltration membrane 29. At this time, the drive motor 21 drives the first bevel gear 23 to rotate through the rotating shaft 22. Since the first bevel gear 23 meshes with two second bevel gears 25, the first bevel gear 23 drives the second bevel gears 25 to rotate synchronously. The two second bevel gears 25 rotate in the opposite direction to each other along with the rotation of the first bevel gear 23. The two second bevel gears 25 drive the two rotating drums 24 to rotate in the opposite direction to each other. The designed rotating drums 24 drive the two inclined ultrafiltration membranes 29 inside to rotate in the opposite direction to each other. The inclined ultrafiltration membranes 29 facilitate the stirring of the culture medium in the two rotating drums 24, so that the opposing flow forces drive the cells to continuously contact the two ultrafiltration membranes 29, promoting filtration while counteracting the flow forces. Large molecular cells and contaminants that are difficult to filter out are evenly distributed on the ultrafiltration membrane 29, making it convenient for subsequent personnel to decompose the large molecular cells and contaminants by delivering the decomposition solution through the upper fixed funnel 210. After ultrafiltration through the two ultrafiltration membranes 29, the required cell molecules enter the connecting tube 212 through the lower fixed funnel 210 and flow out through the diversion mechanism 3. A sealing groove 28 is provided to stagger the close ends of the two rotating cylinders 24 to seal them and prevent culture medium leakage. A limiting groove 26 and a limiting ring 27 are provided to limit the rotation of the two rotating cylinders 24. The drive motor 21 drives the two rotating cylinders 24 to rotate in opposite directions through the first bevel gear 23. The counter-rotating rotating cylinders 24 drive the inclined ultrafiltration membrane 29 to stir the culture medium, forming a counter-current force in the culture medium. This promotes rapid and frequent contact of cells with the ultrafiltration membrane 29. Compared with unidirectional rotation, this speeds up the filtration of the device to a certain extent and improves the practicality of the device.

[0034] By setting up a diversion mechanism 3, one of the two outlet pipes 33 is fixedly connected to the culture medium containing qualified cells, and the other outlet pipe 33 is fixedly connected to the waste discharge or recycling pipe. After the ultrafiltration mechanism 2 completes the ultrafiltration of cells in the culture medium, the operator rotates the rotating handle 36 to drive the diversion cylinder 34 to rotate inside the fixed cylinder 32, aligning the two connecting holes 35 on the diversion mechanism 3 with the connecting pipe 212 and the outlet pipe 33 for collecting qualified cells, so that the culture medium containing qualified cells in the ultrafiltration mechanism 2 enters the diversion cylinder 34 through the connecting pipe 212, and then flows from the diversion cylinder 34 into the outlet pipe 33 to complete the collection process. When it is necessary to clean the pollutants or large molecular cells that are difficult to filter on the ultrafiltration membrane 29, the decomposition liquid is transported into the two rotating cylinders 24 to decompose the pollutants and large molecular cells. The mixed decomposition liquid forms waste liquid, which is transported into the connecting pipe 212 by the high-pressure pump 211. At this time, the operator... The operator rotates the handle 36, causing the diverter 34 to align the connection hole 35 with the connection pipe 212 and the discharge pipe 33 connecting the waste discharge or return pipe. Waste liquid enters the diverter 34 through the connection pipe 212 and is then discharged from the discharge pipe 33, completing the diversion of the device. This facilitates the cleaning of contaminants and macromolecular cells on the ultrafiltration membrane 29, preventing accumulation that could affect the filtration of the ultrafiltration membrane 29. Several grooves 37 are provided on the fixed plate 31 and the rotating handle 36, allowing the operator to observe the grooves 37 and align them with their corresponding connection holes 35. This allows the operator to selectively align two connection holes 35 with the connection pipe 212 and a specific discharge pipe 33 by rotating the handle 36, separating and discharging culture medium containing qualified cells and waste liquid. This facilitates subsequent processing and further improves the practicality of the device.

[0035] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0036] The preferred embodiments of this utility model disclosed above are merely illustrative of the present utility model. These preferred embodiments do not exhaustively describe all details, nor do they limit the utility model to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of this utility model, thereby enabling those skilled in the art to better understand and utilize it. This utility model is limited only by the claims and their full scope and equivalents.

Claims

1. An ultrafiltration device for laboratory research, characterized in that, It includes an insulating shell (1), on which an ultrafiltration mechanism (2) and a flow splitting mechanism (3) are provided; The ultrafiltration mechanism (2) includes a reverse component, a limiting component, and an ultrafiltration component. The reverse component includes a motor (21) fixedly connected to the inner wall of the heat insulation shell (1). The output end of the motor (21) is fixedly connected to a rotating shaft (22). The left end of the rotating shaft (22) is fixedly connected to a first bevel gear (23). The inner wall of the heat insulation shell (1) is rotatably connected to two rotating cylinders (24). The outer walls of the two rotating cylinders (24) are fixedly connected to second bevel gears (25). The two second bevel gears (25) mesh with the first bevel gears (23).

2. The ultrafiltration device for laboratory research according to claim 1, characterized in that, The limiting component includes two limiting grooves (26) opened on the inner wall of the heat insulation shell (1). The inner walls of the two limiting grooves (26) are rotatably connected to limiting rings (27). The sides of the two limiting rings (27) that are close to each other are fixedly connected to the sides of the two rotating cylinders (24) that are far apart from each other.

3. The ultrafiltration device for laboratory research according to claim 2, characterized in that, The ultrafiltration assembly includes sealing grooves (28) that are opened on the sides of the two rotating cylinders (24) that are close to each other. The inner walls of the two sealing grooves (28) are rotatably connected to each other. An ultrafiltration membrane (29) is fixedly connected to the inner wall of each of the two rotating cylinders (24). A fixed funnel (210) is rotatably connected to the inner wall of each of the two rotating cylinders (24).

4. The ultrafiltration device for laboratory research according to claim 3, characterized in that, The two fixed funnels (210) extend to the top and bottom surfaces of the insulation shell (1) and are fixedly connected to the insulation shell (1). A high-pressure pump (211) is fixedly connected to the bottom surface of the insulation shell (1). The top output end of the high-pressure pump (211) is fixedly connected to the bottom end of the fixed funnel (210) located on the lower side. A connecting pipe (212) is fixedly connected to the front output end of the high-pressure pump (211).

5. The ultrafiltration device for laboratory research according to claim 4, characterized in that, The diversion mechanism (3) includes a diversion component and an adjustment component. The diversion component includes a fixing plate (31) fixedly connected to the front side of the heat insulation shell (1). A fixing cylinder (32) is fixedly connected to the bottom surface of the fixing plate (31). The outer wall of the fixing cylinder (32) is fixedly connected to the front end of the connecting pipe (212).

6. The ultrafiltration device for laboratory research according to claim 5, characterized in that, Two discharge pipes (33) are fixedly connected to the outer wall of the fixed cylinder (32), and a diverter cylinder (34) is rotatably connected to the inner wall of the fixed cylinder (32). Two connection holes (35) are opened on the outer wall of the diverter cylinder (34).

7. The ultrafiltration device for laboratory research according to claim 6, characterized in that, The adjustment assembly includes a rotating handle (36) fixedly connected to the top surface of the diverter (34). The top end of the rotating handle (36) extends to the top surface of the fixing plate (31) and is rotatably connected to the inner wall of the fixing plate (31). Several grooves (37) are provided on the top surface of the fixing plate (31) and the outer wall of the rotating handle (36).

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