Corn basal stem rot section sampling storage device

Abstract

The utility model relates to corn stem base rot research technical field especially corn stem base rot part sampling storage device, including transparent casing, still including cutting casing, transparent casing top fixedly connected with cutting casing, the partition board that is equipped between transparent casing and cutting casing, the partition board fixedly connected on transparent casing, the through -hole that is equipped on the partition board, cutting casing fixedly connected with connecting hinge, connecting hinge other end fixedly connected with arc plate, arc plate both ends are equipped with two clamping slots, and the cutting blade swing joint is equipped on the clamping slot, the utility model discloses through arc plate drive cutting blade to corn stem and sample, make sample directly fall into the disease department storage cavity, realized sampling and storage integration structure, can effectively avoid sample pollution, simultaneously, the sealing structure can also prevent pathogenic bacteria from spreading to the environment in the sampling process, reduce the risk of cross infection, and through the touchless operation design, significantly reduce the risk of researchers occupational exposure.

Description

Technical Field

[0001] This utility model relates to the field of research technology on maize stalk base rot, and in particular to a sampling and storage device for maize stalk base rot. Background Technology

[0002] Corn stalk rot is a soil-borne disease caused by pathogens such as Pythium and Fusarium. It mainly affects the base of the corn stalk and the root system, with symptoms being particularly pronounced during the grain-filling to waxy maturity stage. Symptoms include browning and softening of the stalk base, leaf blight or yellowing, and in severe cases, lodging or even death of the entire plant, seriously impacting corn yield. To further study the pathogenesis and control methods of this disease, researchers have developed a specialized sampling and storage device for corn stalk rot. Equipped with professional cutting tools to ensure sampling accuracy, the sealed design prevents sample contamination and facilitates safe transportation. This provides a reliable sample guarantee for subsequent pathogen identification, screening of resistant varieties, and development of integrated control technologies, which is of great significance for promoting research on corn disease control.

[0003] Traditional corn stalk rot sampling and storage devices cannot achieve an integrated structure for sampling and storage. Direct contact with diseased samples may introduce external microorganisms or cross-contamination, interfering with the original state of pathogens and leading to deviations in laboratory identification results. This affects the judgment of disease type and severity. Exposure of diseased parts to air may also accelerate the reproduction of saprophytic fungi, mask the characteristics of the original pathogens, increase diagnostic complexity, exacerbate the risk of pathogen transmission, and even threaten the health of operators.

[0004] Therefore, to address the problems of traditional storage devices failing to achieve an integrated structure for sampling and storage, leading to sample contamination, distorted identification results, increased risk of pathogen transmission, and reduced operational safety and efficiency, a contactless, rapid sampling and storage device for corn stalk rot can be designed to solve these issues. Utility Model Content

[0005] To overcome the problems of traditional storage devices being unable to achieve an integrated structure for sampling and storage, leading to sample contamination, distorted identification results, increased risk of pathogen transmission, and reduced operational safety and efficiency.

[0006] The technical solution of this utility model is as follows: a sampling and storage device for corn stalk base rot, including a transparent shell; and a cutting shell. The cutting shell is fixedly connected to the top of the transparent shell. A partition plate is provided between the transparent shell and the cutting shell. The partition plate is fixedly connected to the transparent shell and has a connecting hole. A connecting hinge is fixedly connected to the cutting shell. An arc-shaped plate is fixedly connected to the other end of the connecting hinge. Two slots are provided at both ends of the arc-shaped plate. Cutting blades are movably connected to the slots. A blade storage cavity is provided on the cutting shell. Several replacement blades are movably connected to the blade storage cavity. A sterilization component is fixedly connected to the center of the cutting shell. The output end of the sterilization component faces the blade storage cavity.

[0007] Preferably, the cutting blade is fixed to the arc-shaped plate via a slot, and the arc-shaped plate is rotatably connected to the cutting housing via a connecting hinge. The desired corn stalk portion is selected and placed between the two cutting blades. The arc-shaped plate is rotated, causing it to drive the cutting blades to cut the corn stalk. The cut corn stalk is clamped between the two cutting blades and rotates into the cutting housing as the arc-shaped plate rotates. Then, the lower cutting blade is pulled out, allowing the corn stalk that has entered the cutting housing to fall into the transparent housing through the connecting hole. If multiple sampling is required, the used cutting blade can be removed, and a replacement blade on the blade storage cavity can be used for sampling again. The used cutting blade can be inserted into the blade storage cavity, sterilized by the sterilization component, and then reused.

[0008] Preferably, the sterilization assembly includes a first power source and sterilization lamps; the first power source is fixedly connected to the center of the cutting housing, and several sterilization lamps are fixedly connected to the side of the first power source near the blade storage cavity, with the sterilization lamps and the first power source being electrically connected.

[0009] Preferably, a knob is rotatably connected to the bottom of the transparent shell, and an inner rotating cylinder is fixedly connected to the knob. The inner rotating cylinder is rotatably connected inside the transparent shell.

[0010] Preferably, the inner rotating cylinder is provided with several disease storage chambers, and a refrigeration component is fixedly connected to the bottom of the disease storage chamber.

[0011] Preferably, the cooling assembly includes a conical mounting bracket, a second power supply, and a miniature cooling chip; the conical mounting bracket is fixedly connected to the bottom of the lesion storage cavity, the second power supply is fixedly connected inside the conical mounting bracket, the miniature cooling chip is fixedly connected above the second power supply, and the second power supply and the miniature cooling chip are electrically connected.

[0012] Preferably, the conical mounting bracket has an annular absorbent cotton pad on its outer side, which is fixedly connected to the bottom of the disease storage cavity.

[0013] Preferably, an inert gas reactor is fixedly connected to the center of the transparent shell, and several delivery pipes are fixedly connected to the output end of the inert gas reactor. The other end of the delivery pipes is fixedly connected to the storage cavity of the lesion.

[0014] Preferably, the cutting housing has an empty cavity, and a rotating top plate is provided above the empty cavity. A fixed top plate is fixedly connected to the top of the cutting housing, and the fixed top plate and the rotating top plate are rotatably connected.

[0015] The beneficial effects of this utility model are:

[0016] Using an arc-shaped plate to drive the cutting blade to sample corn stalks, the sample falls directly into the disease storage cavity, realizing an integrated sampling and storage structure. This effectively avoids sample contamination and significantly reduces the risk of interference from exogenous microorganisms. At the same time, the sealed structure can prevent pathogens from spreading into the environment during the sampling process, reducing the risk of cross-infection. Furthermore, the contactless operation design significantly reduces the risk of occupational exposure for researchers and reduces the possibility of human-induced disease transmission. Attached Figure Description

[0017] Figure 1 The diagram shown is a schematic representation of the overall three-dimensional structure of this utility model.

[0018] Figure 2 The diagram shown is a schematic cross-sectional view of the overall structure of this utility model.

[0019] Figure 3 The diagram shown is a cross-sectional view of the transparent shell structure of this utility model.

[0020] Figure 4 The diagram shown is a bottom view cross-sectional view of the cutting shell structure of this utility model;

[0021] Figure 5 The diagram shown is a front cross-sectional view of the cutting shell structure of this utility model.

[0022] Explanation of reference numerals in the attached drawings: 1. Transparent shell; 2. Cutting shell; 201. Blade storage chamber; 202. Empty chamber; 3. Divider plate; 301. Connecting hole; 4. Connecting hinge; 5. Arc-shaped plate; 501. Slot; 6. Cutting blade; 7. Replacement blade; 801. First power supply; 802. Sterilization lamp; 9. Fixed top plate; 10. Rotating top plate; 11. Knob; 12. Inner rotating cylinder; 1201. Disease storage chamber; 1301. Conical mounting bracket; 1302. Second power supply; 1303. Miniature cooling chip; 14. Annular absorbent cotton sheet; 15. Inert gas reactor; 16. Delivery pipe. Detailed Implementation

[0023] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0024] Corn stalk rot is a serious soil-borne disease that severely damages corn production. Its pathogens are complex and mainly include various pathogenic microorganisms such as Pythium and Fusarium. These pathogens mainly infect the base of the corn stalk and roots, and show obvious symptoms of damage during the critical growth period of corn—from grain filling to waxy maturity.

[0025] Infected plants initially develop water-soaked lesions at the base of the stem. These lesions gradually expand and turn dark brown to blackish-brown. The internal tissues of the stem undergo soft rot and necrosis. As the disease progresses, the plant's vascular system is damaged, leading to obstructed water and nutrient transport. The leaves exhibit typical symptoms of wilting or yellowing. In severe cases, affected plants are highly susceptible to lodging under the influence of wind, rain, or other external forces, and may even die completely, resulting in severe yield losses. According to agricultural statistics, in years when the disease is prevalent, corn stem rot can cause a 20%-30% reduction in yield, and in severely affected fields, it can even lead to total crop failure, causing huge economic losses to the corn industry.

[0026] In the study of maize stalk base rot, the collection and preservation of diseased samples is a fundamental step in carrying out various research tasks. However, traditional sampling methods have many technical defects, which have brought considerable trouble to the research work.

[0027] The most prominent problem is that traditional methods are difficult to standardize and regulate the sampling process. Researchers usually need to use ordinary knives or scissors for manual cutting. This method cannot guarantee the consistency of the sampling location and depth each time, and it is also easy to cause mechanical damage to the sample. Since the typical symptoms of corn stalk rot often appear in the 1st to 3rd nodes of the stalk base, and the boundary between diseased and healthy tissue is the most valuable part for research, deviations in the sampling location will directly affect the accuracy of subsequent experimental results. In practice, sampling personnel without professional guidance often miss key diseased tissue or collect severely rotten necrotic tissue, which will reduce the research value of the sample.

[0028] Another major drawback of traditional sampling methods is their inability to effectively prevent sample contamination. In open sampling, diseased tissues are directly exposed to the air and are highly susceptible to contamination by various microorganisms in the environment. Saprophytic fungi in the field, airborne microorganisms, and even bacteria carried on the operator's gloves or tools can all contaminate the sample. These contaminants can interfere with the isolation and identification of the target pathogens. In particular, fast-growing saprophytic fungi often mask the slow-growing target pathogens on the culture medium, causing researchers to miss key pathogenic strains. In addition, contamination can also affect the results of molecular biology experiments, such as the appearance of nonspecific bands during PCR amplification or affecting the quality of sequencing data.

[0029] Sample preservation is also a weak point of traditional methods. If the collected pathogenic tissue is not properly preserved, it will quickly decompose. In the hot and humid field environment, the quality of the sample may decline significantly within a few hours after collection. Tissue decay not only leads to a decrease in the activity of pathogens, but also causes the degradation of biological macromolecules such as DNA and RNA, which seriously affects the results of subsequent pathogen identification, pathogenicity determination and molecular biology experiments. Traditional preservation methods are difficult to meet the needs of modern research.

[0030] Biosafety issues are equally important. Some pathogens causing corn stalk rot, especially certain Fusarium species, can produce toxic secondary metabolites. In traditional open sampling, these pathogens and their toxins may cause occupational exposure through the operator's respiratory tract, skin wounds, etc., posing a potential threat to the health of researchers. More seriously, pathogens contaminated on sampling tools or gloves may be unintentionally carried to other healthy fields, becoming a new source of disease transmission. Field surveys have found that if tools used for sampling using traditional methods are not thoroughly disinfected, the detection rate of pathogens on their surfaces remains high, which fully demonstrates that the risk of cross-contamination cannot be ignored.

[0031] From a systematic perspective, traditional sampling methods still have problems with sample identification and management. In large-scale field surveys, the lack of a standardized sample identification system can easily lead to sample confusion, causing many inconveniences for subsequent data analysis and research. The absence or incorrect recording of important metadata such as sample collection time, location, and field information will reduce the scientific value of the research data. In addition, inconsistent sampling standards adopted by different researchers will also affect the comparability and reproducibility of research results, which is not conducive to the accumulation and sharing of knowledge.

[0032] These technical deficiencies have negatively impacted several aspects of research on maize stalk rot. In pathogen identification, contaminated samples may lead to erroneous results, affecting the accurate understanding of the pathogen's population structure. In disease resistance breeding, inconsistent sampling can affect the comparability of resistance evaluations across different varieties, reducing breeding efficiency. In disease epidemiology research, sample quality issues can affect the success rate of pathogen isolation and culture, thus interfering with the accurate judgment of disease occurrence patterns. In the field of molecular mechanism research, degraded nucleic acid samples can lead to a decline in experimental data quality and even erroneous research conclusions.

[0033] It is worth noting that the limitations of traditional sampling methods can also affect the practical application value of research results. Research conclusions based on flawed samples may mislead the formulation of disease control strategies. For example, if the dominant pathogen species are incorrectly identified due to sample contamination, it may lead to inappropriate selection of control agents, which not only wastes resources but may also delay the best time for control. Similarly, if the resistance of disease-resistant varieties is misjudged due to sampling problems in the breeding of disease-resistant varieties, it will affect the breeding process and may even lead to the misjudgment and loss of excellent germplasm resources.

[0034] Please see Figures 1-5 This utility model provides an embodiment of a corn stalk base rot sampling and storage device, including a transparent shell 1 and a cutting shell 2. The cutting shell 2 is fixedly connected to the top of the transparent shell 1. A partition plate 3 is provided between the transparent shell 1 and the cutting shell 2. The partition plate 3 is fixedly connected to the transparent shell 1 and has a connecting hole 301. A connecting hinge 4 is fixedly connected to the cutting shell 2. An arc-shaped plate 5 is fixedly connected to the other end of the connecting hinge 4. Two slots 501 are provided at both ends of the arc-shaped plate 5. A cutting blade 6 is movably connected to the slots 501. A blade storage cavity 201 is provided on the cutting shell 2. Several replacement blades 7 are movably connected to the blade storage cavity 201. A sterilization component is fixedly connected to the center of the cutting shell 2. The output end of the sterilization component faces the blade storage cavity 201. The cutting blade 6 is passed through... The slot 501 is fixed on the arc plate 5. The arc plate 5 is rotatably connected to the cutting housing 2 through the connecting hinge 4. Select the desired corn stalk part and place it between the two cutting blades 6. Rotate the arc plate 5 so that the arc plate 5 drives the cutting blades 6 to cut the corn stalk. The cut corn stalk is clamped between the two cutting blades 6. As the arc plate 5 rotates into the cutting housing 2, the lower cutting blade 6 is pulled out, so that the corn stalk that has entered the cutting housing 2 falls into the transparent housing 1 through the connecting hole 301. If multiple sampling is required, the used cutting blade 6 can be removed and the replacement blade 7 on the blade storage cavity 201 can be used for sampling again. The used cutting blade 6 can be inserted into the blade storage cavity 201, sterilized by the sterilization component, and then reused.

[0035] Please see Figures 2-5In this embodiment, the sterilization assembly includes a first power supply 801 and sterilization lamps 802. The first power supply 801 is fixedly connected to the center of the cutting housing 2. Several sterilization lamps 802 are fixedly connected to the side of the first power supply 801 near the blade storage cavity 201. The sterilization lamps 802 and the first power supply 801 are electrically connected. The first power supply 801 outputs current to the sterilization lamps 802, causing them to light up and sterilize the replacement blades 7 in the blade storage cavity 201, preventing cross-contamination. A rotating mechanism is rotatably connected to the bottom of the transparent housing 1. A knob 11 is fixedly connected to an inner rotating cylinder 12, which is rotatably connected inside a transparent shell 1. By rotating the knob 11, the operator causes the inner rotating cylinder 12 to rotate inside the transparent shell 1, thus storing multiple sections of corn stalks. The inner rotating cylinder 12 has several diseased part storage chambers 1201. A refrigeration component is fixedly connected to the bottom of the diseased part storage chamber 1201. After sampling, the corn stalks fall into the diseased part storage chamber 1201, and then the refrigeration component lowers the temperature in the diseased part storage chamber 1201 to prevent the diseased parts from rotting.

[0036] Please see Figures 1-5In this embodiment, the cooling assembly includes a conical mounting bracket 1301, a second power supply 1302, and a micro-cooling chip 1303. The conical mounting bracket 1301 is fixedly connected to the bottom of the diseased tissue storage cavity 1201. The second power supply 1302 is fixedly connected inside the conical mounting bracket 1301, and the micro-cooling chip 1303 is fixedly connected above the second power supply 1302. The second power supply 1302 and the micro-cooling chip 1303 are electrically connected. Fixing the conical mounting bracket 1301 to the bottom of the diseased tissue storage cavity 1201 protects the second power supply 1302 while separating the exudate from the corn stalk. The second power supply 1302 provides current to the micro-cooling chip 1303, causing the micro-cooling chip 1303 to lower the internal temperature of the diseased tissue storage cavity 1201, achieving low-temperature storage. An annular absorbent cotton sheet 14 is provided on the outer side of the conical mounting bracket 1301 and is fixedly connected to the bottom of the diseased tissue storage cavity 1201, enabling automatic absorption of the exudate from the corn stalk. The liquid flows along the outer wall of the conical mounting frame 1301 to the bottom of the disease storage cavity 1201, where it is absorbed by the annular absorbent cotton pad 14 to prevent liquid accumulation. An inert gas reactor 15 is fixedly connected to the center of the transparent shell 1. Several delivery pipes 16 are fixedly connected to the output end of the inert gas reactor 15. The other end of the delivery pipes 16 is fixedly connected to the disease storage cavity 1201. After all the corn stalk samples are stored, the inert gas reactor 15 generates inert gas, which is then delivered to the disease storage cavity 1201 through the delivery pipes 16 to create an anaerobic environment. The cutting shell 2 has an empty cavity 202, and a rotating top plate 10 is provided above the empty cavity 202. A fixed top plate 9 is fixedly connected to the top of the cutting shell 2. The fixed top plate 9 and the rotating top plate 10 are rotatably connected. The empty cavity 202 in the cutting shell 2 is used to store other supplies such as gloves required for sampling. The empty cavity 202 is opened and closed by pushing the rotating top plate 10 to rotate on the fixed top plate 9.

[0037] During operation, the cutting blade 6 is fixed to the arc plate 5 via the slot 501. The arc plate 5 is rotatably connected to the cutting housing 2 via the connecting hinge 4. The desired corn stalk is selected and placed between the two cutting blades 6. The arc plate 5 is rotated, causing it to drive the cutting blades 6 to cut the corn stalk. The cut corn stalk is clamped between the two cutting blades 6. As the arc plate 5 rotates into the cutting housing 2, the lower cutting blade 6 is pulled out, allowing the corn stalk in the cutting housing 2 to fall into the disease storage cavity 1201 of the transparent housing 1 through the connecting hole 301. The exudate from the corn stalk slides down the slope of the conical mounting bracket 1301 and flows onto the annular absorbent cotton sheet 14 to prevent liquid accumulation. At the same time, the second power supply 1302 provides current to the micro-cooling chip 1303, causing the micro-cooling chip 1303 to lower the internal temperature of the disease storage cavity 1201, achieving low-temperature storage. If multiple sampling is required, the used cutting blade 6 can be removed. Using the replacement blade 7 on the blade storage chamber 201, rotate it to take samples again. The used cutting blade 6 can be inserted into the blade storage chamber 201. By rotating the knob 11, the operator drives the inner rotating cylinder 12 to rotate inside the transparent shell 1, replacing it with a new disease storage chamber 1201 for sampling again. The used cutting blade 6 can be inserted into the blade storage chamber 201. The first power supply 801 outputs current to the sterilization lamp 802, causing the sterilization lamp 802 to light up and sterilize the replacement blade 7 in the blade storage chamber 201 to prevent cross-contamination. After all the corn stalk samples are stored, the inert gas reactor 15 generates inert gas, which is transported to the disease storage chamber 1201 through the delivery pipe 16 to create an anaerobic environment. An empty chamber 202 is also set in the cutting shell 2 to store other sampling supplies such as gloves. The empty chamber 202 is opened and closed by pushing the rotating top plate 10 to rotate on the fixed top plate 9.

[0038] Through the above steps, the arc plate 5 drives the cutting blade 6 to sample the corn stalk, allowing the sample to fall directly into the disease storage cavity 1201. This achieves an integrated sampling and storage structure, effectively avoiding sample contamination and significantly reducing the risk of interference from exogenous microorganisms. At the same time, the sealed structure prevents pathogens from spreading into the environment during sampling, reducing the risk of cross-infection. Furthermore, the contactless operation design significantly reduces the risk of occupational exposure for researchers and the possibility of human-induced disease transmission. This addresses the problem that traditional storage devices cannot achieve an integrated sampling and storage structure, leading to sample contamination, distorted identification results, increased risk of pathogen transmission, and reduced operational safety and efficiency.

Claims

1. A sampling and storage device for corn stalk base rot, comprising a transparent shell (1); characterized in that: It also includes a cutting shell (2), a cutting shell (2) fixedly connected above a transparent shell (1), a partition plate (3) between the transparent shell (1) and the cutting shell (2), the partition plate (3) fixedly connected to the transparent shell (1), the partition plate (3) having a connecting hole (301), a connecting hinge (4) fixedly connected to the cutting shell (2), an arc plate (5) fixedly connected to the other end of the connecting hinge (4), two slots (501) at both ends of the arc plate (5), a cutting blade (6) movably connected to the slots (501), a blade storage cavity (201) on the cutting shell (2), several replacement blades (7) movably connected to the blade storage cavity (201), and a sterilization component fixedly connected to the center of the cutting shell (2), with the output end of the sterilization component facing the blade storage cavity (201).

2. The corn stalk base rot sampling and storage device according to claim 1, characterized in that: The sterilization assembly includes a first power source (801) and a sterilization lamp (802); the first power source (801) is fixedly connected to the center of the cutting housing (2), and several sterilization lamps (802) are fixedly connected to the side of the first power source (801) near the blade storage cavity (201), and the sterilization lamps (802) and the first power source (801) are electrically connected.

3. The corn stalk base rot sampling and storage device according to claim 1, characterized in that: A knob (11) is rotatably connected to the bottom of the transparent shell (1), and an inner rotating cylinder (12) is fixedly connected to the knob (11). The inner rotating cylinder (12) is rotatably connected inside the transparent shell (1).

4. The corn stalk base rot sampling and storage device according to claim 3, characterized in that: The inner rotating cylinder (12) is provided with several disease storage chambers (1201), and a refrigeration component is fixedly connected to the bottom of the disease storage chamber (1201).

5. The corn stalk base rot sampling and storage device according to claim 4, characterized in that: The cooling assembly includes a conical mounting bracket (1301), a second power supply (1302), and a miniature cooling chip (1303); the bottom of the lesion storage cavity (1201) is fixedly connected to the conical mounting bracket (1301), the second power supply (1302) is fixedly connected inside the conical mounting bracket (1301), the miniature cooling chip (1303) is fixedly connected above the second power supply (1302), and the second power supply (1302) and the miniature cooling chip (1303) are electrically connected.

6. The corn stalk base rot sampling and storage device according to claim 5, characterized in that: The tapered mounting bracket (1301) has an annular absorbent cotton pad (14) on its outer side, and the annular absorbent cotton pad (14) is fixedly connected to the bottom of the disease storage cavity (1201).

7. The corn stalk base rot sampling and storage device according to claim 6, characterized in that: An inert gas reactor (15) is fixedly connected to the center of the transparent shell (1). Several delivery pipes (16) are fixedly connected to the output end of the inert gas reactor (15). The other end of the delivery pipes (16) is fixedly connected to the disease storage cavity (1201).

8. The sampling and storage device for corn stalk base rot according to claim 1, characterized in that: The cutting housing (2) has an empty cavity (202), and a rotating top plate (10) is provided above the empty cavity (202). A fixed top plate (9) is fixedly connected to the top of the cutting housing (2), and the fixed top plate (9) and the rotating top plate (10) are rotatably connected.

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