A low-temperature storage and transportation device for umbilical cord mesenchymal stem cells

Abstract

The utility model discloses a kind of umbilical cord mesenchymal stem cell low-temperature warehousing transfer devices, it is related to technical field.The utility model barrel is plugged with heat preservation foam cover, the cavity of heat preservation foam cover is plugged with inner tube, L type pipe is connected to inner tube near bottom position, there is a one-week installation round hole on the inner tube cover of inner tube top with center line, filter box is arranged at the center position of inner tube cover, fixed mounting is saved tube in installation round hole, sample placement is plugged in saved tube, sample bottle is clamped in sample placement tube, saved tube is equipped with air release pipe on the side wall that exposes inner tube cover top, filter box is multi-faceted box body, inner tube is injected with liquid nitrogen from L type pipe top and L type pipe top is plugged with plug.The utility model does not easily leak through liquid nitrogen in the process of transportation, nitrogen exhaust process, through sample tube, not only cooling is carried out to sample tube, but also effective nitrogen can be discharged, prevent inner tube internal pressure too big, can carry out multiple sample tube preservation, and preservation effect is good.

Description

Technical Field

[0001] This utility model belongs to the field of stem cell cryogenic transport technology, and in particular relates to a cryogenic storage and transport device for umbilical cord mesenchymal stem cells. Background Technology

[0002] Cell cryopreservation involves placing cells in a low-temperature environment to reduce cellular metabolism and facilitate long-term storage. Umbilical cord mesenchymal stem cells (UC-MSCs) are cryopreserved in liquid nitrogen at -196°C, temporarily detaching them from their growth state while preserving their characteristics. Umbilical cord mesenchymal stem cells are pluripotent stem cells found in the umbilical cord tissue of newborns, primarily derived from Wharton's jelly, a gel-like substance rich in water and extracellular matrix. However, cryopreservation is crucial during transport; transporting them using ice packs can easily damage stem cells, leading to inactivation and inability to differentiate. Furthermore, liquid nitrogen containers cannot hold multiple sample tubes during transport, and the constant movement of liquid nitrogen during transport causes rapid evaporation, making cryopreservation impossible during long-distance transport.

[0003] To address these issues, we provide a cryogenic storage and transport device for umbilical cord mesenchymal stem cells. Utility Model Content

[0004] The purpose of this invention is to provide a cryogenic storage and transport device for umbilical cord mesenchymal stem cells, which uses an inner cylinder containing liquid nitrogen to preserve and transport samples, solving the problems of existing liquid nitrogen being unable to preserve multiple samples and the easy volatilization of liquid nitrogen during the preservation process.

[0005] To solve the above-mentioned technical problems, this utility model is achieved through the following technical solution:

[0006] This utility model relates to a cryogenic storage and transport device for umbilical cord mesenchymal stem cells, comprising a cylindrical body and a cylindrical cover; the cylindrical body is filled with an insulating foam sleeve, the insulating foam sleeve has a cavity, and the insulating foam sleeve has a pipe groove on the side wall of the cavity; an inner cylinder is filled into the cavity of the insulating foam sleeve, and an L-shaped tube is connected to the bottom of the inner cylinder, the vertical end of the L-shaped tube is parallel to the inner cylinder and the top of the L-shaped tube is higher than the top of the inner cylinder, the L-shaped tube is clamped in the pipe groove, and the inner cylinder cover at the top of the inner cylinder has a circumferential mounting hole along the center line, and a filter box is located at the center of the inner cylinder cover, and a filter is fixed in the mounting hole. The filter box contains a storage tube, the bottom of which extends into the inner cylinder near the bottom, and the top of which protrudes from the inner cylinder cover. A sample placement tube is inserted into the storage tube, and a sample bottle is secured within the sample placement tube. A vent pipe is located on the side wall of the storage tube above the inner cylinder cover. The filter box is a multi-faceted box, with a connecting pipe on each side wall corresponding to a vent pipe. The connecting pipe and the corresponding vent pipe are connected by a flexible hose. Liquid nitrogen is injected into the inner cylinder from the top of the L-shaped tube, and the top of the L-shaped tube is plugged. The cylinder cover is screwed onto the top of the cylinder, and the cylinder cover has a circumferential vent hole.

[0007] The present invention is further configured such that liquid inlet holes are evenly distributed on the lower half of the side wall of the storage tube, and a limiting sleeve is fitted on the storage tube near the top, with the bottom of the limiting sleeve attached to the upper surface of the inner cylinder cover.

[0008] The present invention is further configured such that the sample placement tube includes a notched tube and a foam plug, the notched tube has an insertion notch at a distance downward from the tube opening, a set of symmetrical arc-shaped clamping plates are fixed on the inner wall of the notched tube facing inward, the top of the notched tube is embedded into the foam plug from the bottom, and the sample bottle is clamped in the arc-shaped clamping plates.

[0009] The present invention is further configured such that the outer diameter of the notched tube is 70-80% of the inner diameter of the storage tube, and the foam plug will not block the vent tube after it is inserted into the storage tube.

[0010] The present invention is further configured such that the top cover of the filter box is a breathable filter plate.

[0011] The present invention is further provided with a U-shaped handle on the outer wall of the cylinder near the top, and a rotating handle is installed on the top surface of the cylinder cover.

[0012] This utility model has the following beneficial effects:

[0013] 1. This utility model fills the inner cylinder with liquid nitrogen, which will not easily leak during transportation. It will only enter the filter box from the vent pipe on each storage tube and then be discharged. The nitrogen leakage is slow, and during the nitrogen discharge process, it passes through the sample tube, which not only cools the sample tube, but also effectively discharges the nitrogen and prevents the pressure inside the inner cylinder from being too high.

[0014] 2. In this invention, each sample tube is stored independently in a preservation tube, allowing for the preservation of multiple sample tubes with good preservation effect and the ability to preserve and transport multiple stem cell samples simultaneously.

[0015] Of course, any product implementing this utility model does not necessarily need to achieve all of the advantages described above at the same time. Attached Figure Description

[0016] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of a cryogenic storage and transport device for umbilical cord mesenchymal stem cells.

[0018] Figure 2 for Figure 1 A schematic diagram of the structure exposed by the explosion of the sample placement tube and storage tube.

[0019] Figure 3 for Figure 1 A schematic diagram showing the top of the cylinder from above and at an angle after the cylinder cover has been removed.

[0020] Figure 4 This is a schematic diagram of the explosion of the cylinder body, the insulating foam sleeve, and the inner cylinder.

[0021] Figure 5 This is a schematic diagram of the notched tube structure.

[0022] The attached diagram lists the components represented by each number as follows:

[0023] 1. Cylinder body; 11. U-shaped handle; 2. Cylinder cap; 21. Rotating handle; 22. Vent hole; 3. Notched tube; 31. Foam plug; 32. Arc-shaped retaining plate; 33. Insertion notch; 4. Sample bottle; 5. Storage tube; 51. Liquid inlet; 52. Limiting sleeve; 53. Vent pipe; 6. Thermal insulation foam sleeve; 61. Pipe groove; 7. Inner cylinder cap; 71. Filter box; 711. Connecting pipe; 8. Inner cylinder; 81. L-shaped tube. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0025] Please see Figure 1-5 This utility model relates to a low-temperature storage and transport device for umbilical cord mesenchymal stem cells, comprising a cylindrical body 1 and a cylindrical cover 2. The cylindrical body 1 is filled with an insulating foam sleeve 6, which has a cavity. A pipe groove 61 is provided on the side wall of the cavity. An inner cylinder 8 is inserted into the cavity of the insulating foam sleeve 6. An L-shaped tube 81 is connected to the inner cylinder 8 near its bottom. The vertical tube of the L-shaped tube 81 is parallel to the inner cylinder 8, and the top of the L-shaped tube 81 extends beyond the top of the inner cylinder 8. The L-shaped tube 81 is secured within the pipe groove 61. A circular mounting hole 72 is provided around the center line on the inner cylinder cover 7 at the top of the inner cylinder 8. A filter box 71 is located at the center of the inner cylinder cover 7, and a filter box 71 is fixedly installed within the circular mounting hole 72. The system includes a storage tube 5, the bottom of which extends into the inner cylinder 8 near the bottom, and the top of which protrudes from the inner cylinder cover 7. A sample placement tube is inserted into the storage tube 5, and a sample bottle 4 is inserted into the sample placement tube. A vent pipe 53 is provided on the side wall of the storage tube 5 above the inner cylinder cover 7. The filter box 71 is a multi-faceted box, and each side wall of the filter box 71 has a connecting pipe 711, which corresponds to a vent pipe 53. The connecting pipe 711 and the corresponding vent pipe 53 are connected by a flexible hose. Liquid nitrogen is injected into the inner cylinder 8 from the top of the L-shaped tube 81 and the top of the L-shaped tube 81 is plugged. The cylinder cover 2 is screwed onto the top of the cylinder 1, and the cylinder cover 2 has a circumferential vent hole 22.

[0026] After umbilical cord mesenchymal stem cells are collected, they are preserved in sample vials 4. One umbilical cord can be divided into several sample vials 4, or several umbilical cords (e.g., several babies born in one day) can be preserved in several sample vials. Before preservation and transportation, liquid nitrogen is poured into L-shaped tubes 81, which then enter the inner cylinder 8. A stopper is inserted into the opening of the L-shaped tube 81, or a screw cap is screwed onto the opening. Then, sample vials 4 are inserted one by one into the sample placement tube, which is then inserted into the preservation tube 5. After filling, the cap 2 is screwed onto the top of the cylinder 1.

[0027] During storage and transportation, liquid nitrogen will evaporate nitrogen gas and absorb a large amount of heat. The nitrogen gas enters the storage tube 5 and is then discharged from the vent pipe 53 of the storage tube 5 to the filter box 71 and out to the outside. In order to prevent gas from flowing back into the storage tube 5, the filter box 71 can filter out impurities and bacteria in the air.

[0028] The lower half of the storage tube 5 has evenly distributed liquid inlet holes 51. The storage tube 5 is fitted with a limiting sleeve 52 near the top, and the bottom of the limiting sleeve 52 is attached to the upper surface of the inner cylinder cover 7.

[0029] The storage tube 5 is inserted into the installation hole 72 with an interference fit, and the gap is welded. After all the storage tubes 5 are installed and welded, the inner cylinder cover 7 is then welded to the opening of the inner cylinder 8.

[0030] The sample placement tube includes a notched tube 3 and a foam plug 31. The notched tube 3 has an insertion notch 33 at a distance downward from the tube opening. A set of symmetrical arc-shaped clamping plates 32 are fixed on the inner wall of the notched tube 3 with the insertion notch 33 inward. The top of the notched tube 3 is embedded into the foam plug 31 from the bottom. The sample bottle 4 is clamped in the arc-shaped clamping plate 32.

[0031] The insertion notch 33 of the notched tube 3 is made by cutting off half of the wall of the notched tube 3. The arc-shaped clamping plate 32 has a certain elasticity, which can clamp the sample bottle 4 after it is inserted, and then clamp the sample bottle 4 to prevent the sample bottle from falling out. Moreover, it also has a certain shock absorption effect during transportation.

[0032] The outer diameter of the notched tube 3 is 70-80% of the inner diameter of the storage tube 5. After the foam plug 31 is inserted into the storage tube 5, the foam plug 31 will not block the vent tube 53.

[0033] Ensure that when nitrogen flows in storage tube 5, it flows through the gap between notch tube 3 and storage tube 5, and then is discharged through vent tube 53.

[0034] The top cover of the filter box 71 is a breathable filter plate. Nitrogen gas is discharged from the breathable filter plate, and when the backflowing air enters the filter box 71, impurities or bacteria are filtered out.

[0035] The outer wall of the cylinder 1 is provided with a U-shaped handle 11 near the top, and the top surface of the cylinder cover 2 is equipped with a rotating handle 21. The cylinder cover 2 is easy to rotate during installation, and the U-shaped handle 11 facilitates handling.

[0036] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0037] The preferred embodiments of this utility model disclosed above are merely illustrative of the present utility model. These preferred embodiments do not exhaustively describe all details, nor do they limit the utility model to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of this utility model, thereby enabling those skilled in the art to better understand and utilize it. This utility model is limited only by the claims and their full scope and equivalents.

Claims

1. A cryogenic storage and transport device for umbilical cord mesenchymal stem cells, comprising a cylindrical body (1) and a cap (2); characterized in that: The inner cylinder (1) is filled with a thermal insulation foam sleeve (6), which has a cavity. The thermal insulation foam sleeve (6) has a pipe groove (61) on the side wall of the cavity. The cavity of the thermal insulation foam sleeve (6) is filled with an inner cylinder (8). An L-shaped tube (81) is connected to the bottom of the inner cylinder (8). The vertical tube of the L-shaped tube (81) is parallel to the inner cylinder (8), and the top of the L-shaped tube (81) is higher than the top of the inner cylinder (8). The L-shaped tube (81) is stuck in the pipe groove (61). The inner cylinder cover (7) at the top of the inner cylinder (8) has a circular hole (72) around the center line. A filter box (71) is provided at the center of the inner cylinder cover (7). A storage tube (5) is fixedly installed in the circular hole (72). The bottom of the storage tube (5) extends into the cavity. Near the bottom of the inner cylinder (8), the top of the storage tube (5) protrudes from the inner cylinder cover (7). The storage tube (5) contains a sample placement tube, and the sample placement tube contains a sample bottle (4). The storage tube (5) has a vent pipe (53) on the side wall above the inner cylinder cover (7). The filter box (71) is a multi-faceted box. Each side wall of the filter box (71) has a connecting pipe (711) and a corresponding vent pipe (53). The connecting pipe (711) and the corresponding vent pipe (53) are connected by a flexible hose. The inner cylinder (8) is filled with liquid nitrogen from the top of the L-shaped tube (81) and the top of the L-shaped tube (81) is plugged with a stopper. The cylinder cover (2) is screwed onto the top of the cylinder (1). The cylinder cover (2) has a circumferential vent hole (22).

2. The cryogenic storage and transport device for umbilical cord mesenchymal stem cells according to claim 1, characterized in that, The storage tube (5) has liquid inlet holes (51) evenly distributed on the lower half of the side wall. The storage tube (5) is fitted with a limiting sleeve (52) near the top. The bottom of the limiting sleeve (52) is attached to the upper surface of the inner cylinder cover (7).

3. The cryogenic storage and transport device for umbilical cord mesenchymal stem cells according to claim 1, characterized in that, The sample placement tube includes a notched tube (3) and a foam plug (31). The notched tube (3) has an insertion notch (33) at a distance downward from the tube opening. A set of symmetrical arc-shaped clamping plates (32) are fixed on the inner wall of the notched tube (3) with the insertion notch (33) facing inward. The top of the notched tube (3) is embedded into the foam plug (31) from the bottom. The sample bottle (4) is clamped in the arc-shaped clamping plate (32).

4. The cryogenic storage and transport device for umbilical cord mesenchymal stem cells according to claim 3, characterized in that, The outer diameter of the notched tube (3) is 70-80% of the inner diameter of the storage tube (5). After the foam plug (31) is inserted into the storage tube (5), the foam plug (31) will not block the vent tube (53).

5. The cryogenic storage and transport device for umbilical cord mesenchymal stem cells according to claim 1, characterized in that, The top cover of the filter box (71) is a breathable filter plate.

6. The cryogenic storage and transport device for umbilical cord mesenchymal stem cells according to claim 1, characterized in that, The outer wall of the cylinder (1) is provided with a U-shaped handle (11) near the top, and the top surface of the cylinder cover (2) is provided with a rotating handle (21).

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