Oligonucleotide-encoded chemical libraries, related systems, devices, and methods for detecting, analyzing, quantifying, and testing biological / genetic material

The use of picowell arrays with bead-bound DNA barcodes and compounds addresses the limitations of current single-cell RNA sequencing methods, enabling efficient high-throughput drug screening and target discovery by ensuring precise compound identification and quantification.

JP7822317B2Active Publication Date: 2026-03-02PLEXIUM INC
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Patent Information

Application Number
JP2022546075
Authority / Receiving Office
JP · JP
Patent Type
Patents
Current Assignee / Owner
Priority Date
2020-05-08
Filing Date
2021-01-28
Publication Date
2026-03-02
Estimated Expiration
2041-01-28

AI Technical Summary

Technical Problem

Current high-throughput single-cell RNA sequencing methods are limited by the lack of efficient methods to separate different drugs into different cells, and traditional assays are constrained by the capacity of microtiter plates, leading to low throughput and inefficiencies in drug screening and target discovery.

Method used

A system using picowell arrays with beads containing identical DNA barcodes and compounds, where DNA barcodes are linked or orthogonal, and beads are dispensed individually into picowells, allowing for high-throughput screening and cellular response analysis.

Benefits of technology

Enables efficient separation and screening of millions of compounds in picowells, facilitating high-throughput drug screening and target discovery without the need for robotics or spatial indexing, and allowing for precise identification and quantification of compound identities.

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Abstract

The present application provides beads bearing covalently bound compounds and covalently bound DNA barcodes, as well as methods for using such beads. The beads bear multiple copies of substantially identical compounds and multiple copies of substantially identical DNA barcodes. The compounds are composed of one or more chemical monomers, and the DNA barcodes take the form of barcode modules, each corresponding to a corresponding chemical monomer and enabling its identification. The nucleic acid barcodes can have linked or independent structures. Methods are provided for sequencing the bead-bound nucleic acid barcodes to cleave the compounds from the beads and evaluate the biological activity of the released compounds.
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