One-step Seedling Cultivation Method of Wheat Anthers

Abstract

The invention discloses a one-step seedling cultivation method for wheat anthers. Wheat young ears whose pollen cells develop to the mid-monocytic stage to the late monocytic stage are selected and pretreated at low temperature (2°C to 4°C) for 2 days; the pretreated wheat Young ears, after routine disinfection, strip the anther tissue and inoculate it on the one-step seedling medium for shading cultivation; when the cultured anther tissue appears callus visible to the naked eye, it is placed under 1500Lx light conditions for one week and then the light intensity is reduced. Increase to 3000Lx ~ 4000Lx, continuous cultivation for two weeks, can produce robust pollen plants with roots and seedlings. The method reduces two necessary technical operation links for inducing adventitious buds and adventitious roots by pollen callus, reduces the culture cost by nearly 50%, shortens the culture days by 40-50 days, and the average induction rate of green seedlings is comparable to the greenness of multi-step seedlings. The average induction rate of the seedlings was the same, the white seedling rate was generally reduced by 10%, and the test-tube seedlings grew robustly, the root system was developed, and there was little or no callus residue in the rhizome connection part, and the transplanted seedlings had a high survival rate.

Description

technical field

[0001] The invention belongs to the wheat cell engineering breeding technology in food crops, and further relates to a one-step seedling cultivation method for wheat anthers. Background technique

[0002] Wheat is one of the most important food crops for humans. The genetic improvement of wheat through various ways is the basis of high quality, high yield and stable yield of wheat. The wheat haploid breeding technology developed in the 1970s has become one of the important modern wheat biotechnological breeding methods. played an important role in. At present, wheat haploid breeding has been using multi-step seedling formation technology, that is, wheat anther tissues are cultured in vitro on dedifferentiation medium to induce dedifferentiation of pollen cells to produce callus, and then callus is induced on redifferentiation medium Differentiate into rootless seedlings, and the third step is to transfer the induced rootless seedlings to the rooting medium...

Examples

Embodiment Construction

[0013] The present invention mainly completes the dedifferentiation and redifferentiation of wheat pollen cells and the formation of complete plants through the improvement and use of the anther culture medium in a one-step culture process without reducing or improving the culture efficiency to a certain extent. The main technical means and measures adopted are:

[0014] 1) A general-purpose W with a low concentration of inorganic salts was chosen 14 culture medium as basic medium.

[0015] 2) The osmotic pressure in the medium is reduced, and the sucrose concentration is reduced from 10% to 6%.

[0016] 3) Added maltose (to promote the rate of green seedlings and reduce the rate of white seedlings);

[0017] 4) Add important organic substances casein, biotin and glutamine to make thiamine hydrochloride (V b1 ) concentration increased to 10mg / L.

[0018] 5) Reasonably match the inducing hormone KT and NAA.

[0019] 6) Low-temperature pretreatment of the anther tissue befo...

technical field

[0001] The invention belongs to the wheat cell engineering breeding technology in food crops, and further relates to a one-step seedling cultivation method for wheat anthers. Background technique

[0002] Wheat is one of the most important food crops for humans. The genetic improvement of wheat through various ways is the basis of high quality, high yield and stable yield of wheat. The wheat haploid breeding technology developed in the 1970s has become one of the important modern wheat biotechnological breeding methods. played an important role in. At present, wheat haploid breeding has been using multi-step seedling formation technology, that is, wheat anther tissues are cultured in vitro on dedifferentiation medium to induce dedifferentiation of pollen cells to produce callus, and then callus is induced on redifferentiation medium Differentiate into rootless seedlings, and the third step is to transfer the induced rootless seedlings to the rooting medium...

Examples

Embodiment Construction

[0013] The present invention mainly completes the dedifferentiation and redifferentiation of wheat pollen cells and the formation of complete plants through the improvement and use of the anther culture medium in a one-step culture process without reducing or improving the culture efficiency to a certain extent. The main technical means and measures adopted are:

[0014] 1) A general-purpose W with a low concentration of inorganic salts was chosen 14 culture medium as basic medium.

[0015] 2) The osmotic pressure in the medium is reduced, and the sucrose concentration is reduced from 10% to 6%.

[0016] 3) Added maltose (to promote the rate of green seedlings and reduce the rate of white seedlings);

[0017] 4) Add important organic substances casein, biotin and glutamine to make thiamine hydrochloride (V b1 ) concentration increased to 10mg / L.

[0018] 5) Reasonably match the inducing hormone KT and NAA.

[0019] 6) Low-temperature pretreatment of the anther tissue befo...

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