Gold magnetic particle labeled chromatography test strip for detecting aflatoxin

A technology of aflatoxin and gold magnetic particles, which is applied in the field of aflatoxin detection tools, can solve problems such as hidden safety hazards, low separation efficiency, complicated separation operations, etc., and achieves the effects of high degree of automation, low detection cost, and improved sensitivity

CN102680695AInactive Publication Date: 2012-09-19XIAN GOLDMAG NANOBIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2012-09-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides immune magnetic particles for purifying an aflatoxin sample, a chromatography test strip manufactured based on the immune magnetic particles and a detection method, aims to solve the technical problems of complex separation operation, low separation efficiency, and high potential safety hazard in the process of purifying and separating the aflatoxin sample, and discloses a method capable of qualitatively and quantitatively detecting the aflatoxin. By the immune magnetic particles for purifying the aflatoxin sample, gold magnetic particles with the particle size of 20 to 200nm serve as a carrier, an aflatoxin monoclonal antibody serves as a ligand, the gold magnetic particles are connected with the aflatoxin antibody through a covalent reaction or the affinity, and the saturation magnetization of the gold magnetic particles is higher than 30emu / g. The aflatoxin antibody labeled by the gold magnetic particles is used for detection of the chromatography test strip, so that visual qualitative detection can be performed, quantitative detection can be performed by detecting magnetic signals, and the detection sensitivity is greatly improved.
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Description

technical field

[0001] The invention relates to aflatoxin detection tools and a detection method, in particular to an immunomagnetic particle used for purifying aflatoxin samples, a chromatographic test strip and a detection method based on the immunomagnetic particle. Background technique

[0002] Aflatoxin (AFT) is a secondary metabolite of fungi, mainly produced by Aspergillus flavus, Aspergillus parasitica and Aspergillus tererii, and widely exists in soil, animals and plants, and various nuts, especially peanuts and walnuts. There are four main types of aflatoxins: B1, B2, G1, and G2, among which B1 is considered to be the strongest carcinogen and mutagen among chemical carcinogens. In order to prevent food contaminated by AFT from entering the human consumption chain, timely detection will be an effective means.

[0003] At present, the methods for determining aflatoxin (AFT) mainly include thin-layer chromatography, high performance liquid chromatography, enzyme-link...

Examples

Embodiment 1

[0048] Embodiment 1 Preparation of gold magnetic particle-labeled chromatographic test strip and the specific process of detecting aflatoxin in food:

[0049] 1] Preparation of gold magnetic particle-labeled antibody

[0050] a. Immobilization of aflatoxin monoclonal antibody on the surface of gold magnetic particles: take 1mg of gold magnetic particles into a 2ml centrifuge tube, shake gently to resuspend. Place on a magnetic separator, magnetically separate, and discard the supernatant. Add 1ml of ultrapure water and shake gently to resuspend. Place on a magnetic separator, magnetically separate, and discard the supernatant. Repeat the operation once. Prepare aflatoxin monoclonal antibody 100ug into a solution with a concentration of 0.1-10mg / ml, add an appropriate amount to gold magnetic particles, and react in a shaker at 25°C and 180r / min for 1h. After the reaction is completed, magnetic separation is performed, and the supernatant is discarded. Add 1ml of wash buffe...