Gas chromatography measuring method of content of nicotine, myosmine, anabasine, neonicotine and conitine in tobacco juice of electronic cigarette

Abstract

The invention relates to a gas chromatography measuring method of the content of nicotine, myosmine, anabasine, neonicotine and conitine in a tobacco juice of an electronic cigarette. The method is characterized by comprising the following steps: preparing a sample solution to be measured by using an ultrasonic extraction method and compounding a mixed standard working solution with a level-7 concentration gradient; performing analysis by utilizing a gas chromatograph matched with a hydrogen flame ionization detector; and performing quantification by using a double internal standard method. The result shows that the nicotine, the myosmine, the anabasine, the neonicotine and the conitine in respective linearity ranges are good in linear relationships (R2 is greater than 0.998), wherein the limit of detection (LOD) ranges from 3.66mu g / g to 17.8mu g / g; the average recovery rate of three standard adding levels is between 97.1% and 104.6%; the relative standard deviation (RSD) is below 3.86%. Thus, the method is high in sensitivity and good in repeatability and recovery rate.

Description

technical field

[0001] The invention relates to the technical field of physical and chemical inspection of electronic cigarette products, in particular to a gas chromatography method for determining the contents of nicotine, mesmin, anonicotine, pseudohormonine and cotinine in electronic cigarette liquid. Background technique

[0002] In recent years, the topic of smoking and health has been widely concerned by people, and smoking is harmful to health has been generally recognized by people. In 2005, China signed the "Framework Convention on Tobacco Control", which provided a legal basis for tobacco control. In 2012, the Chinese government issued the "China Tobacco Control Plan (2012-2015)", proposing to create a smoke-free environment and implement a comprehensive ban on smoking in public places. Electronic cigarettes (e-cigarettes), also known as electronic nicotine delivery systems (ENDS), are a new form of nicotine intake that looks similar to cigarettes. Over the years...

Examples

Embodiment 1

[0032] 1. Instruments, reagents and instrument working conditions

[0033] 1) Instrument

[0034] SCION 456-GC gas chromatograph with hydrogen flame ionization detector (GC-FID, Bruker Daltonic, USA); AE163 electronic balance (sensitivity: 0.0001 g, Mettler, Switzerland); ultrasonic generator (KQ-700DB CNC ultrasonic cleaner).

[0035] 2) Reagents

[0036] N-heptadecane, 4,4'-bipyridine, nicotine, mesmin, anonicotinoid, pseudohortinine, and cotinine are all standard products; methanol (chromatographically pure, TEDIA, USA).

[0037] 3) Gas chromatography conditions. Chromatographic column: HP-50+ capillary column (30 m × 0.32 mm × 0.25 μm); detector temperature: 250 ℃; inlet temperature: 250 ℃; carrier gas: nitrogen (purity ≥ 99.999%), constant flow Flow rate: 2.0 mL / min; injection volume: 1 μL, splitless injection; heating program: initial temperature of 80 °C, hold for 1 min, increase to 260 °C at a rate of 15 °C / min, and hold for 6 min.

[0038] 2. Preparation of stand...

Embodiment 2

[0057] According to the measurement method described in Example 1, an electronic cigarette liquid B was selected, and the contents of nicotine, mesmin, neonicotinoid, pseudobasine and cotinine were respectively 1.09×10 4 μg / g, 15.5 μg / g, 43.4 μg / g, 136.20 μg / g and 13.3 μg / g.

Embodiment 3

[0059] According to the measurement method described in Example 1, an electronic cigarette liquid C was selected, and the contents of nicotine, mesmin, neonicotinoid, pseudobasine and cotinine were respectively 1.97×10 4 μg / g, 13.8 μg / g, 38.13 μg / g, 125.33 μg / g and 10.65 μg / g.

technical field

[0001] The invention relates to the technical field of physical and chemical inspection of electronic cigarette products, in particular to a gas chromatography method for determining the contents of nicotine, mesmin, anonicotine, pseudohormonine and cotinine in electronic cigarette liquid. Background technique

[0002] In recent years, the topic of smoking and health has been widely concerned by people, and smoking is harmful to health has been generally recognized by people. In 2005, China signed the "Framework Convention on Tobacco Control", which provided a legal basis for tobacco control. In 2012, the Chinese government issued the "China Tobacco Control Plan (2012-2015)", proposing to create a smoke-free environment and implement a comprehensive ban on smoking in public places. Electronic cigarettes (e-cigarettes), also known as electronic nicotine delivery systems (ENDS), are a new form of nicotine intake that looks similar to cigarettes. Over the years...

Examples

Embodiment 1

[0032] 1. Instruments, reagents and instrument working conditions

[0033] 1) Instrument

[0034] SCION 456-GC gas chromatograph with hydrogen flame ionization detector (GC-FID, Bruker Daltonic, USA); AE163 electronic balance (sensitivity: 0.0001 g, Mettler, Switzerland); ultrasonic generator (KQ-700DB CNC ultrasonic cleaner).

[0035] 2) Reagents

[0036] N-heptadecane, 4,4'-bipyridine, nicotine, mesmin, anonicotinoid, pseudohortinine, and cotinine are all standard products; methanol (chromatographically pure, TEDIA, USA).

[0037] 3) Gas chromatography conditions. Chromatographic column: HP-50+ capillary column (30 m × 0.32 mm × 0.25 μm); detector temperature: 250 ℃; inlet temperature: 250 ℃; carrier gas: nitrogen (purity ≥ 99.999%), constant flow Flow rate: 2.0 mL / min; injection volume: 1 μL, splitless injection; heating program: initial temperature of 80 °C, hold for 1 min, increase to 260 °C at a rate of 15 °C / min, and hold for 6 min.

[0038] 2. Preparation of stand...

Embodiment 2

[0057] According to the measurement method described in Example 1, an electronic cigarette liquid B was selected, and the contents of nicotine, mesmin, neonicotinoid, pseudobasine and cotinine were respectively 1.09×10 4 μg / g, 15.5 μg / g, 43.4 μg / g, 136.20 μg / g and 13.3 μg / g.

Embodiment 3

[0059] According to the measurement method described in Example 1, an electronic cigarette liquid C was selected, and the contents of nicotine, mesmin, neonicotinoid, pseudobasine and cotinine were respectively 1.97×10 4 μg / g, 13.8 μg / g, 38.13 μg / g, 125.33 μg / g and 10.65 μg / g.

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