Marking method for hybrid seed production of hot peppers

Abstract

The invention relates to the technical field of hybrid seed production of plants, and particularly discloses a marking method for the hybrid seed production of hot peppers. The method comprises the following steps: (1) determining the cross pollination period of the hot peppers; (2) marking female parent plants selected for the hybrid seed production; (3) collecting pollen of the cross-breeding male parents; (4) performing emasculation cross-breeding and flower sleeving isolation; (5) marking cross-breeding flower positions; (6) marking seed fruits; and (7) picking fruits and processing. According to the marking method for the hybrid seed production of the hot peppers, the mixing of fruits with different varieties during picking can be completely eradicated, the purity of seeds can be ensured, and the accuracy of experimental research in a later period and the consistency of production seeds can be ensured.

Description

technical field

[0001] The invention relates to the technical field of plant hybrid seed production, in particular to a marking method for capsicum hybrid seed production. Background technique

[0002] Capsicum is a kind of heterofloral crop with hermaphroditic flowers, and most of them are self-crossing. At the same time, due to the influence of wind and insect vectors, the cross-flowering rate is also high, which can reach 15-20% and above. In terms of pepper production, at this stage, except for the individual uses of pepper fruits, conventional non-species multi-directional hybrids are used for transformation. In capsicum production, testing, and hybrid seed production for multiple good traits gene transfer and aggregation, due to the defects of the current marking method, when harvesting seeds, pepper fruits are often mixed with self-bred fruits and due to wind or insect vectors. Other hybrid fruits produced by other factors, and can not be checked when picking and tes...

Examples

Embodiment 1

[0026] Capsicum hybrid seed production labeling method, comprises the steps:

[0027] 1) Determine the best time for cross-pollination of pepper: the best start time for cross-pollination is when the third flower of the male sterile female material plant of pepper "9537" blooms.

[0028] 2) Marking of the female parent plant selected for hybrid seed production: use a colored tag to hang on the conspicuous branch of the "9537" female parent plant selected for seed production and mark it, and mark the number of the female parent with a marker on the tag "9537".

[0029] 3) Pollen collection of the hybrid male parent: According to the planned seed production combination, the anthers of the mature but not yet flowered male parent "4536" were picked in the afternoon of 1 day before pollination, and wrapped in sulfuric acid paper (bag) marked with the name "4536" , placed indoors in a cool, dry place with no wind and spread out, allowing the anthers to loosen their powder naturally...

Embodiment 2

[0035] Capsicum hybrid seed production labeling method, comprises the steps:

[0036] 1) Determine the best period for cross-pollination of pepper: the best start period for cross-pollination begins when the fourth flower of the female parent plant of pepper "4631" blooms.

[0037] 2) Marking of the female parent plant selected for hybrid seed production: use a colored tag to hang on the conspicuous branch of the "4631" female parent plant selected for seed production and mark it, and use a marker pen to mark the female parent plant number " 4631".

[0038] 3) Pollen collection of the hybrid male parent: According to the planned seed production combination, the anthers of the mature but unflowered male parent "5326" were picked in the afternoon of 1 day before pollination, and the anthers of the male parent "5326" marked with the name of the male parent "5326" were picked. ) wrapped well, spread out indoors in a cool, dry place with no wind, let the anthers loosen naturally a...

Embodiment 3

[0044] Capsicum hybrid seed production labeling method, comprises the steps:

[0045] 1) Determine the best time for cross-pollination of pepper: the best time for cross-pollination begins when the fifth flower of the female parent plant of pepper "4637" blooms.

[0046] 2) Marking of the female parent plant selected for hybrid seed production: use a colored tag to hang on the conspicuous branch of the "4637" female parent plant selected for seed production and mark it, and mark the female parent number on the tag with a marker pen 4637".

[0047] 3) Pollen collection of the hybrid male parent: According to the planned seed production combination, pick the mature anthers of the male parent "4307" that has not yet bloomed 1 day before pollination, and use the sulfuric acid paper (bag) marked with the name of the male parent "4307" ) wrapped well, spread out indoors in a cool, dry place with no wind, let the anthers loosen naturally and then collect pollen.

[0048] 4), emascu...

technical field

[0001] The invention relates to the technical field of plant hybrid seed production, in particular to a marking method for capsicum hybrid seed production. Background technique

[0002] Capsicum is a kind of heterofloral crop with hermaphroditic flowers, and most of them are self-crossing. At the same time, due to the influence of wind and insect vectors, the cross-flowering rate is also high, which can reach 15-20% and above. In terms of pepper production, at this stage, except for the individual uses of pepper fruits, conventional non-species multi-directional hybrids are used for transformation. In capsicum production, testing, and hybrid seed production for multiple good traits gene transfer and aggregation, due to the defects of the current marking method, when harvesting seeds, pepper fruits are often mixed with self-bred fruits and due to wind or insect vectors. Other hybrid fruits produced by other factors, and can not be checked when picking and tes...

Examples

Embodiment 1

[0026] Capsicum hybrid seed production labeling method, comprises the steps:

[0027] 1) Determine the best time for cross-pollination of pepper: the best start time for cross-pollination is when the third flower of the male sterile female material plant of pepper "9537" blooms.

[0028] 2) Marking of the female parent plant selected for hybrid seed production: use a colored tag to hang on the conspicuous branch of the "9537" female parent plant selected for seed production and mark it, and mark the number of the female parent with a marker on the tag "9537".

[0029] 3) Pollen collection of the hybrid male parent: According to the planned seed production combination, the anthers of the mature but not yet flowered male parent "4536" were picked in the afternoon of 1 day before pollination, and wrapped in sulfuric acid paper (bag) marked with the name "4536" , placed indoors in a cool, dry place with no wind and spread out, allowing the anthers to loosen their powder naturally...

Embodiment 2

[0035] Capsicum hybrid seed production labeling method, comprises the steps:

[0036] 1) Determine the best period for cross-pollination of pepper: the best start period for cross-pollination begins when the fourth flower of the female parent plant of pepper "4631" blooms.

[0037] 2) Marking of the female parent plant selected for hybrid seed production: use a colored tag to hang on the conspicuous branch of the "4631" female parent plant selected for seed production and mark it, and use a marker pen to mark the female parent plant number " 4631".

[0038] 3) Pollen collection of the hybrid male parent: According to the planned seed production combination, the anthers of the mature but unflowered male parent "5326" were picked in the afternoon of 1 day before pollination, and the anthers of the male parent "5326" marked with the name of the male parent "5326" were picked. ) wrapped well, spread out indoors in a cool, dry place with no wind, let the anthers loosen naturally a...

Embodiment 3

[0044] Capsicum hybrid seed production labeling method, comprises the steps:

[0045] 1) Determine the best time for cross-pollination of pepper: the best time for cross-pollination begins when the fifth flower of the female parent plant of pepper "4637" blooms.

[0046] 2) Marking of the female parent plant selected for hybrid seed production: use a colored tag to hang on the conspicuous branch of the "4637" female parent plant selected for seed production and mark it, and mark the female parent number on the tag with a marker pen 4637".

[0047] 3) Pollen collection of the hybrid male parent: According to the planned seed production combination, pick the mature anthers of the male parent "4307" that has not yet bloomed 1 day before pollination, and use the sulfuric acid paper (bag) marked with the name of the male parent "4307" ) wrapped well, spread out indoors in a cool, dry place with no wind, let the anthers loosen naturally and then collect pollen.

[0048] 4), emascu...