PCR primer sets, probe sets and kits for the detection of various respiratory viruses

A primer set and respiratory technology, applied in the direction of recombinant DNA technology, microbial measurement/inspection, and resistance to vector-borne diseases, etc., can solve the problems that cannot meet the detection of respiratory pathogens, and achieve the effect of low cost, accurate detection and easy operation

CN105713993BActive Publication Date: 2018-05-25SHENZHEN YILIFANG BIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2018-05-25

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Abstract

The invention discloses a PCR primer set for detecting multiple respiratory viruses, a probe set and a kit. All sequences of PCR primers are shown in SEQ ID NO.1-SEQ ID NO.18. Probes comprise at least two of molecular beacon probes for the nine respiratory viruses, wherein the two ends of the probes are provided with fluorophores and quenching groups respectively. All sequences are shown in SEQ ID NO.19-SEQ ID NO.27. The invention further discloses a PCR reaction solution containing the PCR primers and the probes and the kit containing the PCR reaction solution for at least one person. By means of the PCR primer set, the probe set and the kit, the nine respiratory viruses can be rapidly and accurately detected in a parting mode.
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Description

technical field

[0001] The invention relates to a PCR primer set, a probe set and a kit for detecting various respiratory viruses. Background technique

[0002] Respiratory tract infection is one of the most common clinical diseases, which can be caused by a variety of pathogens, including bacteria, viruses, mycoplasma, chlamydia, etc. Among them, acute respiratory tract infection (Acute Respiratory Tract Infection, ARTI) is an important cause of illness and death in children worldwide. The World Health Organization (WHO) published an article in the "Lancet" magazine in 2005 and reported that in 2000-2003, among the 10.6 million children under the age of 5 who died each year, 54% of them died from infectious diseases, of which 54% died from pneumonia. Children accounted for 19%. ARTI is strictly defined as all respiratory infections, however, in reality, the acute lower respiratory tract accounts for the majority of severe respiratory diseases, accounting for 20% of all de...

Examples

Embodiment Construction

[0037] The invention relates to a PCR primer set, a probe set, a PCR reaction solution and a kit for typing and detecting various respiratory viruses. The present invention will be described in further detail below in combination with specific implementation methods and accompanying drawings. It should be emphasized that the following description is only exemplary and not intended to limit the scope of the invention and its application.

[0038] According to the embodiments of the present invention, the PCR primer set, probe set, PCR reaction solution and kit for the detection of multiple respiratory viruses will be used for the typing detection of at least two of the following nine respiratory viruses, respectively: A Influenza Type I, Influenza B, Respiratory Syncytial Virus, Parainfluenza Type I, Parainfluenza Type II, Parainfluenza Type III, Coronavirus, Rhinovirus C, and Human Metapneumovirus.

[0039] In some embodiments, the conserved gene fragment of the respiratory R...