Method for improving alkaloid content of dendrobium huoshanense protocorm

Abstract

The invention provides a method for improving the alkaloid content of dendrobium huoshanense protocorm, wherein dendrobium huoshanense protocorm is used as a material, and the alkaloid content of the dendrobium huoshanense protocorm is increased by using a Phomopsis elicitor. According to the present invention, the influence of the Phomopsis elicitor on the alkaloid content of the dendrobium huoshanense protocorm during suspension culture is researched, and the purpose of the present invention is to provide the new idea for the large-scale industrial production of alkaloids and other medicinal components from dendrobium huoshanense, and the theoretical basis for the medicine source development of dendrobium huoshanense.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a method for increasing the content of alkaloids in protocorms of Dendrobium huoshanense. Background technique

[0002] Huoshan Dendrobium ( Dendrobium huoshanense C. Z. Tang et S. J. Cheng), commonly known as rice dendrobium, is a perennial epiphytic herb of the genus Dendrobium orchidaceae. It is produced in Huoshan County, Anhui Province in the Dabie Mountains. One of the nine kinds of medicinal Dendrobium recorded in the Pharmacopoeia of the Republic. It has many functions such as promoting body fluid and benefiting stomach, improving eyesight and clearing throat, anti-cancer and anti-cancer, and relieving fatigue. Dendrobium Huoshanense is often epiphytic with mosses, stone reeds and other herbaceous plants, and mostly grows in cool and humid places, so its growth area is extremely narrow, coupled with its low natural reproductive ability, slow growth rate, and hi...

Examples

Embodiment Construction

[0018] 1 Materials and methods

[0019] 1.1 Test material

[0020] The material used in this experiment is the protocorm of Dendrobium huoshanense induced in vitro and preserved for a long time, which comes from the Horticultural Plant Bioengineering Institute of Fujian Agriculture and Forestry University. After taking it out, place it in 1 / 2 solid medium for subculture, and screen out protocorms with good growth and no differentiation as test materials.

[0021] 1.2 Test method

[0022] 1.2.1 Liquid culture

[0023] Inoculate the protocorm of Dendrobium huoshanense that was cultured and screened on 1 / 2 MS solid medium in the early stage in 1 / 2 MS liquid medium, under the condition of light intensity of about 2000 lx, use a shaker at 110 r / min Protocorms of Dendrobium Huoshanense were subjected to liquid shaking culture at a rotating speed of 100°C. The pH of the medium was adjusted to 5.8 before high pressure. The culture vessel was a 200 mL Erlenmeyer flask with a liquid ...

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a method for increasing the content of alkaloids in protocorms of Dendrobium huoshanense. Background technique

[0002] Huoshan Dendrobium ( Dendrobium huoshanense C. Z. Tang et S. J. Cheng), commonly known as rice dendrobium, is a perennial epiphytic herb of the genus Dendrobium orchidaceae. It is produced in Huoshan County, Anhui Province in the Dabie Mountains. One of the nine kinds of medicinal Dendrobium recorded in the Pharmacopoeia of the Republic. It has many functions such as promoting body fluid and benefiting stomach, improving eyesight and clearing throat, anti-cancer and anti-cancer, and relieving fatigue. Dendrobium Huoshanense is often epiphytic with mosses, stone reeds and other herbaceous plants, and mostly grows in cool and humid places, so its growth area is extremely narrow, coupled with its low natural reproductive ability, slow growth rate, and hi...

Examples

Embodiment Construction

[0018] 1 Materials and methods

[0019] 1.1 Test material

[0020] The material used in this experiment is the protocorm of Dendrobium huoshanense induced in vitro and preserved for a long time, which comes from the Horticultural Plant Bioengineering Institute of Fujian Agriculture and Forestry University. After taking it out, place it in 1 / 2 solid medium for subculture, and screen out protocorms with good growth and no differentiation as test materials.

[0021] 1.2 Test method

[0022] 1.2.1 Liquid culture

[0023] Inoculate the protocorm of Dendrobium huoshanense that was cultured and screened on 1 / 2 MS solid medium in the early stage in 1 / 2 MS liquid medium, under the condition of light intensity of about 2000 lx, use a shaker at 110 r / min Protocorms of Dendrobium Huoshanense were subjected to liquid shaking culture at a rotating speed of 100°C. The pH of the medium was adjusted to 5.8 before high pressure. The culture vessel was a 200 mL Erlenmeyer flask with a liquid ...

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