Glioma diagnostic marker circ6:22020339|22020542 and its application

A technology for glioma and exosomes, applied in biochemical equipment and methods, microbial measurement/testing, etc., can solve the problems of poor postoperative survival rate, poor treatment effect, high recurrence rate, etc., and achieve fast and accurate The effect of mastering the patient's condition, high diagnostic and prognostic analysis value, and convenient and accurate diagnosis

CN107557471BActive Publication Date: 2020-06-05XIANGYA HOSPITAL CENT SOUTH UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2020-06-05

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Abstract

The invention discloses a glioma diagnostic marker Circ6: 22020339|22020542 and an application. According to the invention, it is first found that an exosome secreted by glioma cells has an obvious enrichment effect on Circ6: 22020339|22020542, and the expression level of Circ6: 22020339|22020542 in the serum exosome of a patient who suffers from glioma is obviously reduced (p<0.0001) than that ofa control group. ROC curve analysis shows that the marker has relatively high diagnostic value (AUC=0.986, p<0.0001, the susceptibility is 100%, and the specificity is 92.9%) on glioma. Therefore, through detection of the expression level of Circ6: 22020339|22020542 in the serum exosome of a patient who suffers from glioma, noninvasive diagnosis can be early fast made to the patient who suffers from glioma.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a serum circRNA marker Circ6:22020339|22020542 for glioma diagnosis, and the application of a reagent for detecting the marker to prepare glioma diagnostic preparations, and a kit. Background technique

[0002] Glioma is the most common malignant neuroepithelial cell tumor in the brain, accounting for 40.49% of intracranial tumors, and is one of the most common brain tumors in adults. From the time of diagnosis, the average life expectancy of patients with glioma is less than five years. At present, there are various treatment techniques for glioma, but due to the characteristics of fast tumor infiltration and invasion, and insensitivity to apoptosis mediated by radiotherapy and chemotherapy, the overall treatment effect is not good, the recurrence rate is high, and the prognosis is poor. Therefore, new treatments are sought. Targets and prognostic indicators are critical. At present...

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Embodiment Construction

[0019] The following embodiments are intended to further illustrate the present invention, rather than limit the present invention.

[0020] 1. Research object

[0021] 1. The two glioma cell lines are U251 and U87 cell lines, and the six primary glioma cell lines are 1216, 1125, 1124B, 1124C, 0128C, and 1104, all provided by the Institute of Cancer, School of Basic Medicine, Central South University. 2. The serum samples of 40 glioma patients were provided by Xiangya Hospital, and the 15 normal serum samples were healthy individuals who underwent community disease screening at the same time. The samples used for the research were collected at the same period, and the conditions of sampling, packaging and storage were the same.

[0022] 2. Research methods

[0023] 1. Extraction of RNA from cells, exosomes secreted by cells, and glioma / normal serum exosomes

[0024] A. Extraction of cellular RNA

[0025] After the above 8 kinds of cells grow to an appropriate density, disc...