A water wheel rotating antibody incubation device

Abstract

The present invention relates to the technical field of biomedical research equipment, and in particular to a water wheel rotating antibody incubation device, comprising a liquid storage tank for containing a blocking liquid and a vertical rotating wheel installed in the liquid storage tank, a plurality of mesh blades extending along its radial direction are installed on the vertical rotating wheel of the water wheel structure, the angles formed by two adjacent mesh blades are the same, and a PVDF membrane is installed on each mesh blade. The present invention is provided with a vertical rotating wheel, mesh blades are arranged on the vertical rotating wheel, and a PVDF membrane is installed on each mesh blade. During the rotation of the vertical rotating wheel, each mesh blade loaded with a PVDF membrane will pass through the blocking liquid carried in the liquid storage tank, and the blocking liquid will be covered on the PVDF membrane, thereby realizing the transfer of proteins, and at the same time, the blocking liquid is poured from top to bottom between the inner ring skeleton and the outer ring skeleton of the vertical rotating wheel through a water pump installed in the liquid storage tank, forming a blocking liquid flowing from top to bottom, and the flowing blocking liquid can realize the transfer of proteins.

Description

Technical Field

[0001] The invention relates to the technical field of biomedical research equipment, and in particular to a water wheel rotating antibody incubation device. Background Art

[0002] In the field of biomedical research, immunoblotting, also known as Western blotting, has been increasingly widely used in molecular biology techniques. It can effectively perform qualitative and semi-quantitative protein analysis on samples to be tested. It mainly includes five steps: electrophoresis, transfer, blocking, hybridization and color development, among which the blocking and hybridization processes are also called antibody incubation processes. In immunoblotting experiments, protein electrophoresis and transfer membrane methods are already very mature, and there are also standardized research equipment and devices on the market. After the target protein is transferred to the transfer membrane, it needs to be further incubated with the antibody solution. The traditional incubation method is to use a hybridization bag or incubation box. The hybridization bag is prone to bubbles during use, resulting in incomplete antibody incubation, and the hybridization bag is easy to break and bring the risk of contamination; the traditional incubation box is a box of general structure, which requires a large amount of antibody. In addition, the size and specifications of the transfer membrane are different, and it is difficult to prepare multiple suitable boxes in reality. Summary of the invention

[0003] The object of the present invention is to provide a water wheel rotating antibody incubation device in view of the deficiencies in the prior art.

[0004] In order to solve the above problems, the present invention provides the following technical solutions:

[0005] A water wheel rotary antibody incubation device comprises a liquid storage tank for containing a blocking liquid and a vertical rotating wheel installed in the liquid storage tank, a bracket for installing the vertical rotating wheel is arranged in the liquid storage tank, a driving motor for driving the vertical rotating wheel to rotate is arranged on the top of the bracket, a control box is arranged on the outer wall of the liquid storage tank, a control circuit board and a power supply are arranged in the control box, the control circuit board is electrically connected to the power supply, the driving motor is electrically connected to the control circuit board through a wire, a switch electrically connected to the control circuit board is arranged outside the control box, and the vertical rotating wheel is a water wheel structure, and a plurality of mesh blades extending along the radial direction thereof are arranged on the vertical rotating wheel of the water wheel structure, the angles formed by two adjacent mesh blades are the same, and a PVDF film is arranged on each mesh blade.

[0006] Preferably, a water pump is installed in the liquid storage tank, and a water supply pipe is installed on the bracket. One end of the water supply pipe is connected to the output end of the water pump, and the other end of the water pump extends upward along the bracket to just above the top of the vertical rotating wheel. The water pump is electrically connected to the control circuit board through a wire.

[0007] Preferably, the vertical impeller includes a turntable body and a blade mounting frame. An axial hole connected to the output end of the driving motor is provided at the center of the turntable body. The blade mounting frame includes an inner ring skeleton, an outer ring skeleton and a closed ring. The inner ring skeleton and the outer ring skeleton are fixedly mounted on the same side of the turntable body and are coaxially arranged therewith. The bottoms of the inner ring skeleton and the outer ring skeleton are fixedly connected to the turntable body. All blades are mounted between the inner ring skeleton and the outer ring skeleton. The outer ring skeleton is a hollow structure, and the closed ring is a disc-shaped structure. The inner ring edge of the closed ring is connected to the top of the inner ring skeleton, and the outer ring edge of the closed ring is connected to the top of the outer ring skeleton.

[0008] Preferably, the outer ring skeleton of the hollow structure includes a lower ring body, an upper ring body and a plurality of connecting edges. The lower ring body and the upper ring body are arranged in parallel. The connecting edges are vertically connected between the upper ring body and the lower ring body. The lower ring is fixedly connected to the turntable body. The distance between two adjacent connecting edges is equal and forms a window for the passage of the sealing liquid.

[0009] Preferably, an outer ring groove is provided on the inner side of each connecting edge, and the outer ring groove extends to the top of the upper ring body. Several inner ring grooves are provided on the outer side of the inner ring skeleton, and the inner ring grooves extend to the top of the inner ring skeleton. Each inner ring groove corresponds to an outer ring groove and forms a group of slots for installing mesh blades.

[0010] Preferably, an inner ring buckle and an outer ring buckle are provided on the inner side of the closed ring, the outer ring buckle of the closed ring buckles with the outer side of the upper ring body of the outer ring skeleton, and a groove is provided on the inner side of the top of the inner ring skeleton, and the inner ring buckle of the closed ring buckles with the groove on the inner ring skeleton.

[0011] Preferably, the bottoms of the inner ring frame and the outer ring frame are fixedly connected to the turntable body by glue.

[0012] Preferably, the closing ring is a plastic part made of transparent material.

[0013] Beneficial effects: A water wheel rotating antibody incubation device of the present invention is provided with a vertical rotating wheel, on which mesh blades are arranged, each mesh blade is installed with a PVDF membrane, and during the rotation of the vertical rotating wheel, each mesh blade loaded with the PVDF membrane will pass through the blocking liquid carried in the liquid storage tank, and the blocking liquid will be covered on the PVDF membrane, thereby realizing the transfer of protein, and at the same time, the blocking liquid is poured from top to bottom between the inner ring skeleton and the outer ring skeleton of the vertical rotating wheel through a water pump installed in the liquid storage tank, forming a blocking liquid flowing from top to bottom, and the flowing blocking liquid can realize the transfer of protein. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 The three-dimensional structure of the present invention is shown in FIG. Figure 1 ;

[0015] Figure 2 The three-dimensional structure of the present invention is shown in FIG. Figure 2 ;

[0016] Figure 3 is a side view of the present invention;

[0017] Figure 4 It is a schematic diagram of the three-dimensional exploded structure of the rotating wheel in the present invention;

[0018] Figure 5 yes Figure 4 The enlarged schematic diagram of point A in the middle;

[0019] Explanation of the reference numerals: liquid storage tank 1, vertical rotating wheel 2, bracket 3, driving motor 4, control box 5, mesh blades 6, water pump 7, water supply pipe 8, turntable body 9, inner ring skeleton 10, inner ring groove 10a, outer ring skeleton 11, upper ring body 11a, lower ring body 11b, connecting edge 11c, outer ring groove 11d, closed ring 12. DETAILED DESCRIPTION

[0020] The specific embodiments of the present invention are further described in detail below in conjunction with the accompanying drawings and embodiments of the specification:

[0021] Reference Figures 1 to 5 A water wheel rotary antibody incubation device shown in the figure comprises a liquid storage tank 1 for containing a blocking liquid and a vertical rotating wheel 2 installed in the liquid storage tank 1, a bracket 3 for installing the vertical rotating wheel 2 is provided in the liquid storage tank 1, a driving motor 4 for driving the vertical rotating wheel 2 to rotate is installed on the top of the bracket 3, a control box 5 is provided on the outer wall of the liquid storage tank 1, a control circuit board and a power supply are installed in the control box 5, the control circuit board is electrically connected to the power supply, the driving motor 4 is electrically connected to the control circuit board through a wire, a switch electrically connected to the control circuit board is installed outside the control box 5, and the vertical rotating wheel 2 is a water wheel structure, and a plurality of mesh blades 6 extending along the radial direction thereof are installed on the vertical rotating wheel 2 of the water wheel structure, the angles formed by two adjacent mesh blades 6 are the same, and a PVDF film is installed on each mesh blade 6.

[0022] A water pump 7 is also installed in the liquid storage tank 1, and a water supply pipe 8 is installed on the bracket 3. One end of the water supply pipe 8 is connected to the output end of the water pump 7, and the other end of the water pump 7 extends upward along the bracket 3 to just above the top of the vertical rotating wheel 2. The water pump 7 is electrically connected to the control circuit board through a wire.

[0023] The vertical impeller 2 includes a turntable body 9 and a blade mounting frame. An axial hole connected to the output end of the driving motor 4 is provided at the center of the turntable body 9. The blade mounting frame includes an inner ring skeleton 10, an outer ring skeleton 11 and a closed ring 12. The inner ring skeleton 10 and the outer ring skeleton 11 are fixedly installed on the same side of the turntable body 9 and are coaxially arranged therewith. The bottoms of the inner ring skeleton 10 and the outer ring skeleton are fixedly connected to the turntable body 9. All blades are installed between the inner ring skeleton 10 and the outer ring skeleton 11. The outer ring skeleton 11 is a hollow structure, and the closed ring 12 is a disc-shaped structure. The inner ring edge of the closed ring 12 is connected to the top of the inner ring skeleton 10, and the outer ring edge of the closed ring 12 is connected to the top of the outer ring skeleton 11.

[0024] The outer ring skeleton 11 of the hollow structure includes a lower ring body 11b, an upper ring body 11a and a plurality of connecting edges 11c. The lower ring body 11b and the upper ring body 11a are arranged in parallel. The connecting edges 11c are vertically connected between the upper ring body 11a and the lower ring body 11b. The lower ring body 11b is fixedly connected to the turntable body 9. The distance between two adjacent connecting edges 11c is equal and constitutes a window for the sealing liquid to pass through.

[0025] The outer ring skeleton 11 is a hollow structure, and the mesh blades 6 can smoothly immerse into the sealing liquid in the liquid storage tank 1 during the rotation process, while also ensuring that the sealing liquid flowing from top to bottom enters between the inner ring skeleton 10 and the outer ring skeleton 11.

[0026] An outer ring groove 11d is provided on the inner side of each connecting edge 11c, and the outer ring groove 11d extends to the top of the upper ring body 11a. A plurality of inner ring grooves 10a are provided on the outer side of the inner ring skeleton 10, and the inner ring grooves 10a extend to the top of the inner ring skeleton 10. Each inner ring groove 10a corresponds to an outer ring groove 11d and constitutes a group of slots for installing the mesh blades 6.

[0027] An inner ring buckle and an outer ring buckle are provided on the inner side of the closed ring 12. The outer ring buckle of the closed ring 12 is buckled with the outer side of the upper ring body 11a of the outer ring skeleton 11. A slot is provided on the inner side of the top of the inner ring skeleton 10. The inner ring buckle of the closed ring 12 is buckled with the slot on the inner ring skeleton 10.

[0028] The closing ring 12 is installed by snap-fitting, which makes it easy to replace the mesh blades 6.

[0029] The bottoms of the inner ring frame 10 and the outer ring frame 11 are fixedly connected to the turntable body 9 by glue.

[0030] The closed ring 12 is a transparent plastic part.

[0031] Beneficial effects: A water wheel rotating antibody incubation device of the present invention is provided with a vertical rotating wheel 2, on which mesh blades 6 are arranged, and each mesh blade 6 is installed with a PVDF membrane. During the rotation of the vertical rotating wheel 2, each mesh blade 6 loaded with the PVDF membrane will pass through the blocking liquid carried in the liquid storage tank 1, and cover the blocking liquid on the PVDF membrane, thereby realizing the transfer of protein. At the same time, the blocking liquid is poured from top to bottom between the inner ring skeleton 10 and the outer ring skeleton 11 of the vertical rotating wheel 2 through a water pump 7 installed in the liquid storage tank 1, forming a blocking liquid flowing from top to bottom, and the flowing blocking liquid can realize the transfer of protein.

[0032] The above description is only a preferred embodiment of the present invention and does not limit the technical scope of the present invention. Therefore, any slight modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A water wheel rotating antibody incubation device, characterized in that: The invention comprises a liquid storage tank (1) for containing a sealing liquid and a vertical rotating wheel (2) installed in the liquid storage tank (1); a bracket (3) for installing the vertical rotating wheel (2) is arranged in the liquid storage tank (1); a driving motor (4) for driving the vertical rotating wheel (2) to rotate is installed on the top of the bracket (3); a control box (5) is arranged on the outer wall of the liquid storage tank (1); a control circuit board and a power supply are installed in the control box (5); the control circuit board is electrically connected to the power supply; the driving motor (4) is electrically connected to the control circuit board through a wire; a switch electrically connected to the control circuit board is installed outside the control box (5); the vertical rotating wheel (2) is a water wheel structure; a plurality of mesh extending in the radial direction of the vertical rotating wheel (2) are installed on the water wheel structure. The vertical rotating wheel (2) comprises a rotating disk body (9) and a blade mounting frame. The rotating disk body (9 ... The inner ring skeleton (10) and the outer ring skeleton (11) are fixedly mounted on the same side of the turntable body (9) and are coaxially arranged therewith. The bottoms of the inner ring skeleton (10) and the outer ring skeleton are fixedly connected to the turntable body (9). All blades are mounted between the inner ring skeleton (10) and the outer ring skeleton (11). The outer ring skeleton (11) is a hollow structure. The closed ring (12) is a disc-shaped structure. The inner ring edge of the closed ring (12) is connected to the top of the inner ring skeleton (10), and the outer ring edge of the closed ring (12) is connected to the top of the outer ring skeleton (11). The outer ring skeleton (11) with a hollow structure includes a lower ring body (11b), an upper ring body (11a) and a plurality of connecting edges (11c). The lower ring body (11b) and the upper ring body (11a) are connected to each other. The upper ring body (11a) and the lower ring body (11b) are arranged in parallel, the connecting ribs (11c) are vertically connected between the upper ring body (11a) and the lower ring body (11b), the lower ring body (11b) is fixedly connected to the turntable body (9), the distance between two adjacent connecting ribs (11c) is equal and they form windows for the sealing liquid to pass through; the inner side of each connecting rib (11c) is provided with an outer ring groove (11d), the outer ring groove (11d) extends to the top of the upper ring body (11a), the outer side of the inner ring skeleton (10) is provided with a plurality of inner ring grooves (10a), the inner ring grooves (10a) extend to the top of the inner ring skeleton (10), each inner ring groove (10a) corresponds to an outer ring groove (11d) and forms a group of slots for mounting the mesh blades (6);An inner ring buckle and an outer ring buckle are provided on the inner side of the closed ring (12), the outer ring buckle of the closed ring (12) is buckled with the outer side of the upper ring body (11a) of the outer ring frame (11), a slot is provided on the inner side of the top of the inner ring frame (10), the inner ring buckle of the closed ring (12) is buckled with the slot on the inner ring frame (10); the bottom of the inner ring frame (10) and the outer ring frame (11) are fixedly connected to the turntable body (9) by glue; the closed ring (12) is a plastic part made of transparent material; each mesh blade loaded with PVDF membrane will pass through the sealing liquid carried in the liquid storage tank, and the sealing liquid will cover the PVDF membrane, thereby realizing the transfer of protein, and at the same time, the sealing liquid will be poured from top to bottom between the inner ring frame and the outer ring frame of the vertical rotating wheel through a water pump installed in the liquid storage tank, forming a sealing liquid flowing from top to bottom, and the flowing sealing liquid can realize the transfer of protein. ;

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