The present invention discloses a bio-enzyme method preparing textile bamboo fibril and the method need not any chemical processing, has simple operation, short processing time and stabilizing product quality. The process is as follows: first of all, cutting bamboo and subdividing bamboo into bamboo strip, then boiling them in boiled water till they are soft, putting out, crushing and hammering into filament, then boiling in pressure cookers, immersing the treated bamboo filament in the solution containing bio-enzyme in order to the lignin, hemicellulose and pectin in bamboo filament can be dissociated by bio-enzyme and preparing the cellulose fiber in bamboo; then washing, bleaching, oiling, softening and opening the bamboo cellulose fiber dissociated by enzyme, then the bamboo fibril to be used for weaving is prepared. The invention solves the problem of stable product quality without any chemical treatment which is to be solved and has not been resolved.