Protein transfer printing core and protein transfer mold system thereof
The modularly designed protein transfer core solves the problems of large precious metal consumption and high maintenance difficulty in existing technologies, achieves efficient arrangement of electrode wires within the effective area, reduces costs, and improves electric field uniformity and protein transfer efficiency.
Patent Information
- Application Number
- CN202510911369.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-02
- Publication Date
- 2025-09-19
AI Technical Summary
The platinum wire electrode structure in the existing wet transfer system results in a large amount of precious metal consumption, high equipment costs, difficulty in maintenance and inability to flexibly configure. In addition, the electrode wiring is routed around the outside of the bracket or to the non-working area, wasting electricity.
The protein transfer core is designed as a split modular structure. The positive electrode assembly and the negative electrode assembly can be detachably installed on the inner wall of the frame. The positive electrode wire and the negative electrode wire form electrodes of a specific shape through the hole array and are arranged only in the effective area to eliminate power waste in the non-working area.
It reduces the use of precious metals, reduces the difficulty of production and maintenance, reduces material waste, improves the electric field uniformity and protein transfer efficiency, and meets the requirements of green chemistry.
Smart Images
Figure CN120668939A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of protein transfer devices, and in particular relates to a protein transfer core and a protein transfer mold system thereof. Background Art
[0002] Western blot (protein immunoblotting) is one of the most common experiments in basic medical research. In a typical Western blot electrophoresis transfer system, the core component is the protein transfer core, which consists primarily of a core body and metal electrodes on either side. For example, in wet transfer systems (such as the Bio-Rad Mini-PROTEAN® series), both the positive and negative electrodes are constructed from a single platinum wire, using high-purity platinum to ensure excellent conductivity and chemical stability. The platinum wire is secured in a polycarbonate or ABS plastic holder, where it is wound back and forth repeatedly, forming a conductive electrode interface. Finally, it is connected to an external power source via a conductive cable to create a stable, uniform electric field between the gel and membrane.
[0003] Existing wet transfer systems typically require a large amount of electrophoresis buffer to be added to the transfer tank to ensure ion conduction and temperature control. Accordingly, the length and number of platinum wire electrodes are often designed to cover the entire width of the transfer tank to avoid uneven transfer and leakage. However, this "full-slot-width" integrated electrode structure results in a significant amount of platinum wire usage, increasing equipment costs and making maintenance more difficult. Platinum wire electrodes are often integrated with brackets, wires, and other related components in a non-removable or tool-removable manner, lacking the ability to quickly replace, perform graded maintenance, or flexibly configure according to experimental needs. Summary of the Invention
[0004] The technical problem to be solved by the present application is to provide a protein transfer core and a protein transfer mold system thereof. The present application can greatly reduce the amount of precious metal used in the positive electrode, avoid the waste of electricity in the "dead zone" where the wiring is routed around the outside of the bracket or the non-working area, and is easy to disassemble, reducing the difficulty of production and maintenance.
[0005] The present application provides a protein transfer core, characterized by comprising: The frame includes at least two pairs of oppositely disposed inner side walls, a first pair of inner side walls having a bearing groove for mounting a rotary mold clamp, and each side wall of the second pair of inner side walls being perpendicular to each side wall of the first pair of inner side walls; A positive electrode assembly, comprising: a positive carrier sheet and a positive electrode wire, wherein the positive carrier sheet is detachably mounted on a first sidewall of the second pair of inner sidewalls, the positive carrier sheet being provided with a plurality of first holes, one end of the positive electrode wire being fixed to the positive carrier sheet, and the other end of the positive electrode wire being sequentially passed through the plurality of first holes to form a positive electrode having a first shape on a surface of the positive carrier sheet facing away from the first sidewall; A negative electrode assembly, comprising: a negative carrier sheet and a negative electrode wire, wherein the negative carrier sheet is detachably mounted on the second sidewall of the second pair of inner sidewalls, the negative carrier sheet being provided with a plurality of second holes, one end of the negative electrode wire being fixed to the negative carrier sheet, and the other end of the negative electrode wire being sequentially passed through the plurality of second holes to form a negative electrode having a second shape on a surface of the negative carrier sheet facing away from the second sidewall; and A pair of electrode connectors are installed on the top of the frame, the other end of the positive electrode wire is fixedly connected to one of the electrode connectors, and the negative electrode wire is fixedly connected to the other electrode connector.
[0006] Furthermore, the first holes are evenly distributed on the positive carrier sheet in an m×n array, and the second holes are evenly distributed on the negative carrier sheet in an a×b array.
[0007] Furthermore, the first shape is a rectangular pulse shape or a rectangular vortex shape, and the second shape is a rectangular pulse shape or a rectangular vortex shape.
[0008] Furthermore, the positive supporting plate includes a vertical plate and a plurality of horizontal plates arranged in parallel and at intervals on the vertical plate, the first holes are provided at both ends of the plurality of horizontal plates, and a pair of first holes are provided at both ends of the vertical plate. The path of the positive wire is: one end is fixed at the first hole on the top side of the vertical plate, and it starts to pass through the first hole on the same side of each horizontal plate in turn, and then changes direction through a pair of first holes on the bottom side of the vertical plate, and passes through the first hole on the other side of each horizontal plate in turn.
[0009] Furthermore, the negative carrier sheet has multiple groups of parallel guide structures, each group of guide structures is composed of a plurality of second holes arranged in a linear shape, and the negative electrode wire passes through each second hole in the guide structure located at the bottom one by one until it reaches the top of the negative carrier sheet, forming the second shape according to a rectangular pulse-shaped winding path.
[0010] Furthermore, the second pair of inner side walls of the frame are each provided with mounting grooves, and the positive bearing sheet and the negative bearing sheet are respectively inserted into the corresponding mounting grooves.
[0011] Furthermore, the top and bottom of each inner side wall of the second pair of inner side walls are provided with the mounting groove.
[0012] Furthermore, the two electrode heads are respectively arranged at the top of each side wall in the first pair of inner side walls, and two guide channels are provided on the frame, which respectively extend to one of the electrode heads, and each guide channel is for one of the positive electrode wire and the negative electrode wire to pass through.
[0013] Furthermore, the positive electrode wire is a platinum wire, and the negative electrode wire is a stainless steel wire.
[0014] Furthermore, the protein transfer core further comprises: two connector covers, which are respectively sleeved on the corresponding electrode connectors and fixed to the frame, and the connector covers are provided with marks.
[0015] Furthermore, the protein transfer core further comprises: a temperature sensing element, wherein the temperature sensing element is connected to the pair of electrode connectors.
[0016] The present application also provides a protein transfer system, comprising the above-mentioned protein transfer core.
[0017] Beneficial effects of this application: The protein transfer core of the present application is formed by winding the positive electrode wire on the positive carrier sheet to form a positive electrode assembly, and winding the negative electrode wire on the negative carrier sheet to form a negative electrode assembly. The positive carrier sheet and the negative carrier sheet are both detachably mounted on the inner side walls opposite to each other in the frame. The positive electrode assembly and the negative electrode assembly are designed as a split modular structure. The corresponding modules can be installed or replaced by manual insertion only, without the need for special tools or disassembly of the entire machine, and without the need for glue. This reduces the difficulty of production and maintenance, and facilitates the recycling of precious metals and non-precious metals on the positive and negative electrode assemblies, reducing costs. The positive electrode wire only needs to be routed through the first holes of the positive carrier sheet to form a positive electrode with a first shape, and the negative electrode wire only needs to be routed through the second holes of the negative carrier sheet to form a negative electrode with a second shape. The arrangement of the first and second holes allows the first and second shapes to be basically located on the side of the positive / negative carrier sheet away from the inner wall of the frame, thereby arranging the electrode wire only in the effective area inside the protein transfer core, eliminating the previous waste of power caused by routing the wire around the outside of the bracket or in the "dead zone" in the non-working area. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings of the embodiments will be briefly introduced below. Obviously, the drawings in the following description only relate to some embodiments of the present disclosure, rather than limiting the present disclosure.
[0019] Figure 1 Shown is a structural diagram of a protein transfer core in one embodiment of the present disclosure; Figure 2 for Figure 1 Middle section view; Figure 3 shows a structural diagram of a framework in one embodiment of the present disclosure; Figure 4 for Figure 3 sectional view of Figure 5 shows a structural diagram of a positive electrode assembly in one embodiment of the present disclosure; Figure 6 shows a structural diagram of a positive electrode assembly in another embodiment of the present disclosure; Figure 7 shows a structural diagram of a positive electrode assembly in another embodiment of the present disclosure; Figure 8 shows a distribution diagram of the second pores in the negative electrode assembly in another embodiment of the present disclosure; Figure 9 A structural diagram showing the distribution of second pores in a negative electrode assembly in another embodiment of the present disclosure is shown; Figure 10 It shows a structural diagram of a joint cover in one embodiment of the present disclosure; Figure 11 A structural diagram of the connector cover at another angle in an embodiment of the present disclosure is shown. DETAILED DESCRIPTION
[0020] To make the purpose, technical solutions, and advantages of the embodiments of the present disclosure more clear, the technical solutions of the embodiments of the present disclosure will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present disclosure. Obviously, the described embodiments are part of the embodiments of the present disclosure, not all of the embodiments. Based on the described embodiments of the present disclosure, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present disclosure.
[0021] Unless otherwise defined, the technical or scientific terms used herein shall have the ordinary meaning understood by persons of ordinary skill in the field to which the present disclosure belongs. The words "first", "second" and similar terms used in the patent application specification and claims of the present disclosure do not indicate any order, quantity or importance, but are only used to distinguish different components. Words such as "include" or "comprise" and similar terms mean that the elements or objects appearing before "include" or "comprise" include the elements or objects listed after "include" or "comprise" and their equivalents, and do not exclude other elements or objects. Words such as "connect" or "connected" and similar terms are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.
[0022] As used in this disclosure, the terms "perpendicular," "parallel," and "identical" include the strict sense of "perpendicular," "parallel," and "identical," as well as "approximately perpendicular," "approximately parallel," and "approximately identical" to encompass certain errors, taking into account the errors associated with the measurement of a particular quantity (i.e., the limitations of the measurement system), and represent an acceptable range of deviation for a particular value as determined by a person of ordinary skill in the art. The "center" in the embodiments of this disclosure may include a position strictly at the geometric center as well as a position approximately centered within a small area around the geometric center. For example, "approximately" can mean within one or more standard deviations, or within 10% or 5% of the value.
[0023] The "length" of each structure used in this disclosure refers to Figure 1 In the left and right directions, "height" refers to Figure 1 In the up and down direction, "width" refers to Figure 1 In the direction perpendicular to the paper, "top" refers to Figure 1 The upper part of the structure diagram, and the "bottom" refers to Figure 1 The lower part of the structure diagram. Length, Height, Width, Top, and Bottom do not change when the diagram orientation changes.
[0024] This embodiment provides a protein transfer core, which includes: The frame includes at least two pairs of oppositely disposed inner side walls, a first pair of inner side walls having a bearing groove for mounting a rotary mold clamp, and each side wall of the second pair of inner side walls being perpendicular to each side wall of the first pair of inner side walls; A positive electrode assembly, comprising: a positive carrier sheet and a positive electrode wire, wherein the positive carrier sheet is detachably mounted on a first sidewall of the second pair of inner sidewalls, the positive carrier sheet being provided with a plurality of first holes, one end of the positive electrode wire being fixed to the positive carrier sheet, and the other end of the positive electrode wire being sequentially passed through the plurality of first holes to form a positive electrode having a first shape on a surface of the positive carrier sheet facing away from the first sidewall; A negative electrode assembly, comprising: a negative carrier sheet and a negative electrode wire, wherein the negative carrier sheet is detachably mounted on the second sidewall of the second pair of inner sidewalls, the negative carrier sheet being provided with a plurality of second holes, one end of the negative electrode wire being fixed to the negative carrier sheet, and the other end of the negative electrode wire being sequentially passed through the plurality of second holes to form a negative electrode having a second shape on a surface of the negative carrier sheet facing away from the second sidewall; and A pair of electrode connectors are installed on the top of the frame, the other end of the positive electrode wire is fixedly connected to one of the electrode connectors, and the negative electrode wire is fixedly connected to the other electrode connector.
[0025] The protein transfer core of the disclosed embodiments is constructed by winding a positive electrode wire around a positive carrier sheet to form a positive electrode assembly, and a negative electrode wire around a negative carrier sheet to form a negative electrode assembly. Both the positive and negative carrier sheets are detachably mounted on opposing inner sidewalls of a frame. The positive and negative electrode assemblies are designed as split modular structures. The corresponding modules can be installed or replaced by manual insertion, without the need for specialized tools, disassembly of the entire device, or the use of glue. This reduces production and maintenance difficulties and facilitates the recycling of precious or non-precious metals in the positive and negative electrode assemblies, thereby reducing costs. Furthermore, the positive electrode wire only needs to be routed through the first holes of the positive carrier sheet to form a positive electrode having a first shape, and the negative electrode wire only needs to be routed through the second holes of the negative carrier sheet to form a negative electrode having a second shape. The arrangement of the first and second holes allows the first and second shapes to be substantially entirely located on the side of the positive and negative carrier sheets away from the inner wall of the frame, thereby arranging the electrode wires only in the effective area inside the protein transfer core, eliminating the wasteful power supply caused by routing the wires around the outside of the bracket or in the "dead zone" of the non-working area.
[0026] This embodiment also provides a protein transfer system, which includes the protein transfer core.
[0027] The protein transfer core and protein transfer system thereof according to some embodiments of the present disclosure are exemplarily described below with reference to the accompanying drawings.
[0028] Figure 1 The structure of the protein transfer core in one embodiment of the present disclosure is shown. Figure 2 for Figure 1 Cross-sectional view of the middle part.
[0029] like Figure 1 、 Figure 2 As shown, this embodiment provides a protein transfer core 100 . The protein transfer core 100 includes: a frame 10 , a positive electrode assembly 20 , a negative electrode assembly 30 and a pair of electrode connectors 40 .
[0030] like Figure 3 As shown, the frame 10 includes at least two pairs of oppositely arranged inner side walls (i.e., a first pair of inner side walls 11 and a second pair of inner side walls 12), the first pair of inner side walls 11 is provided with a bearing groove 13 for installing the mold rotation clamp, and each side wall of the second pair of inner side walls 12 is perpendicular to each side wall of the first pair of inner side walls 11.
[0031] Specifically, 1 to 4 bearing slots 13 may be provided.
[0032] In some embodiments, the outer sidewalls of the frame 10 corresponding to the second pair of inner sidewalls 12 are marked with "film" and "glue," respectively. This eliminates the need for confusing red and black markings, allowing users to quickly install the transfer sandwich based on the markings on the frame 10. This reduces the likelihood of incorrectly oriented transfer sandwich installation, potentially leading to experimental failures.
[0033] The frame 10 is generally a rectangular parallelepiped. The frame 10 may also be a prism with a polygonal cross section.
[0034] Figure 4 for Figure 3 sectional view of .
[0035] like Figure 4 As shown, in some embodiments, the second pair of inner side walls 12 of the frame 10 are both provided with mounting grooves (1211, 1221). Specifically, the second pair of inner side walls 12 include a first side wall 121 and a second side wall 122. The first side wall 121 is provided with the mounting groove 1211, and the second side wall 122 is provided with the mounting groove 1221.
[0036] like Figure 4 As shown, in some embodiments, each inner side wall of the second pair of inner side walls 12 has a mounting groove on its top and bottom. For example, the first side wall 121 has a mounting groove 1211 on its top and bottom, and the second side wall 122 has a mounting groove 1221 on its top and bottom.
[0037] In some embodiments, a guide channel is provided on the frame, specifically, two guide channels are provided, one is a positive wire guide channel 141, and the other is a negative wire guide channel 142. The positive wire guide channel 141 is used to guide the positive wire 22 so that the positive wire 22 is connected to the positive electrode connector, and the negative wire guide channel 142 is used to guide the negative wire 32 so that the negative wire 32 is connected to the negative electrode connector.
[0038] Figure 5 A structural diagram of a positive electrode assembly in an embodiment of the present disclosure is shown. Figure 6 A structural diagram of a positive electrode assembly in another embodiment of the present disclosure is shown.
[0039] like Figure 5 、 Figure 6 As shown, the positive electrode assembly 20 includes: a positive carrier sheet 21 and a positive electrode wire 22. The positive carrier sheet 21 is detachably mounted on the first side wall 121 of the second pair of inner side walls 12. A plurality of first holes 211 are provided on the positive carrier sheet 21. One end of the positive electrode wire 22 is fixed on the positive carrier sheet 21, and the other end passes through the plurality of first holes 211 in sequence to form a positive electrode having a first shape on the surface of the positive carrier sheet 21 facing away from the first side wall 121.
[0040] The cathode assembly can be manufactured as a single module, allowing for individual installation and replacement within the frame. This modular design not only facilitates disassembly and recycling, but also allows for the rapid extraction and reuse of precious metals such as platinum wire from the cathode assembly, in line with the requirements of green chemistry and sustainable laboratory development. This reduces waste of disposable materials, extends equipment life, and reduces the environmental burden of laboratory operations.
[0041] In some embodiments, the positive supporting piece 21 is inserted into the mounting groove 1211. Specifically, both ends of the positive supporting piece 21 are inserted into the mounting grooves 1211 at the top and bottom of the first side wall 121, respectively.
[0042] In some embodiments, the first holes 211 are evenly distributed in an m×n array on the positive carrier sheet 21. The winding pattern and density can be selected according to experimental requirements, which can further reduce the amount of precious metal used in the positive electrode assembly, thereby reducing production costs and material input.
[0043] Specifically, the distance between two adjacent first holes 211 is less than or equal to 1 mm. Optimally, the distance between two adjacent first holes 211 is 1 mm.
[0044] Specifically, the height of the positive supporting piece 21 is the same as the distance between the top and bottom mounting grooves 1211 on the first side wall 121, and the length of the positive supporting piece 21 is the same as the length of the top and bottom mounting grooves 1211 on the first side wall 121. The positive supporting piece 21 is made of an elastic material with a certain elasticity. During installation, the elasticity of the positive supporting piece 21 allows the two ends of the positive supporting piece 21 to be inserted into the top and bottom mounting grooves 1211 on the first side wall 121, respectively, so that the positive supporting piece 21 is fixed in the mounting grooves 1211 (i.e., Figure 2 As shown, the positive supporting piece 21 will not move up, down, left, or right after being installed in the installation groove 1211).
[0045] In some embodiments, as Figure 5 As shown, the first shape is a rectangular pulse shape. Specifically, in the rectangular pulse shape, the width of each pulse is the same, and the distance between two adjacent pulses is the same as the pulse width.
[0046] In some embodiments, as Figure 6 As shown, the first shape is a rectangular spiral shape. Specifically, in the rectangular spiral shape, the distances between all adjacent parallel lines are the same.
[0047] Specifically, when the positive electrode wire 22 is routed, one end is fixed to the positive carrier sheet 21, specifically by tying a knot. The other end is routed in a predetermined shape. The routing requirements are: when the positive electrode wire 22 is not turning, it is always located on the side of the positive carrier sheet 21 facing the negative carrier sheet 31. When the positive electrode wire 22 turns, it needs to pass through the positive carrier sheet 21 to the side facing away from the negative carrier sheet 31. When the positive electrode wire 22 passes through the positive carrier sheet 21 to the side facing away from the negative carrier sheet 31, it immediately passes back through the adjacent first hole 211, which is consistent with the routing shape, to the side of the positive carrier sheet 21 facing the negative carrier sheet 31. Therefore, only a small portion of the positive electrode wire is located on the side of the positive carrier sheet 21 facing away from the negative carrier sheet 31. Since the distance between two adjacent first holes is very small, the positive electrode wire 22 is essentially located on the side of the positive carrier sheet 21 facing the negative carrier sheet 31. Therefore, basically all electrode wires immersed in the electrophoresis fluid can participate in the formation of a uniform electric field, greatly improving the efficiency and uniformity of protein transfer, and reducing the waste caused by the wiring being routed around the outside of the frame, causing the electric field generated by this part to exceed the range of the transfer medium and be unable to be used for protein transfer.
[0048] Specifically, the positive support sheet 21 can be a rectangular piece with the first holes 211 distributed throughout the rectangular piece. Alternatively, the positive support sheet 21 includes a rectangular piece and two mounting members 215 mounted on the rectangular piece, with the first holes 211 distributed throughout the rectangular piece. The two mounting members 215 are located at both ends of the upper portion of the rectangular piece, facilitating insertion of the positive support sheet 21 into the mounting slots 1211 at the top and bottom of the first sidewall 121. Specifically, the two mounting members 215 are integrally formed with the rectangular piece.
[0049] It should be noted that the positive carrier sheet 21 may also have other structures, and the first shape formed by the positive electrode wire 22 on the positive carrier sheet 21 may also be a special non-parallel geometric pattern. It is sufficient to ensure that uniform electric field lines are formed between the positive and negative electrodes and that the electric field lines are perpendicular to the surface of the gel-transfer film.
[0050] For example, Figure 7 A structural diagram of a positive electrode assembly in another embodiment of the present disclosure is shown.
[0051] In some embodiments, as Figure 7 As shown, the positive support sheet 21 includes a vertical sheet 212 and multiple horizontal sheets 213. The multiple horizontal sheets 213 are arranged parallel to and spaced apart from the vertical sheet 212. Each end of the multiple horizontal sheets 213 is provided with a first hole 211, and each end of the vertical sheet 212 is provided with a pair of first holes 211. The positive electrode wire 22 has the following path: one end of the positive electrode wire 22 is fixed to the first hole 211 on the top side of the vertical sheet 212. After passing through the first hole 211 on the same side of each horizontal sheet 213, it then changes direction through the pair of first holes 211 on the bottom side of the vertical sheet 212 and passes through the first hole 211 on the other side of each horizontal sheet 213.
[0052] Specifically, all the transverse pieces 213 have different lengths, and the intervals between two adjacent transverse pieces 213 are different. The transverse pieces 213 are symmetrically arranged with respect to the vertical piece 212 .
[0053] Specifically, if Figure 7 As shown, the positive carrier sheet 21 includes a vertical sheet 212 and four horizontal sheets 213. The routing path of the positive electrode wire 22 is as follows: one end of the positive electrode wire 22 is fixed at the first hole at the upper end of the vertical sheet 212, and the free end of the positive electrode wire 22 is routed from the positive carrier sheet 21 toward the side of the negative carrier sheet 31, through the first through hole on the left side of the topmost horizontal sheet 213 to the side of the positive carrier sheet 21 facing away from the negative carrier sheet 31, then through the first through hole on the left side of the horizontal sheet adjacent to the topmost, then through the first through hole on the left side of the horizontal sheet at the bottom, then through the first through hole on the left side of the horizontal sheet adjacent to the bottommost horizontal sheet, and then through the left side of the vertical sheet 212 at the bottom end. The first through hole on the side of the vertical sheet 212 is passed to the side of the positive carrier sheet 21 facing away from the negative carrier sheet 31, and then, it passes through the first through hole on the right side of the lower end of the vertical sheet 212 to the side of the positive carrier sheet 21 facing the negative carrier sheet 31, and then, it passes through the first through hole on the right side of the transverse sheet adjacent to the bottom transverse sheet, and then, it passes through the first through hole on the right side of the transverse sheet at the bottom, and then, it passes through the first through hole on the right side of the transverse sheet adjacent to the top, and finally, after forming the first shape through the first through hole on the right side of the top transverse sheet, it is connected to the electrode connector 40 for connecting the positive electrode through the positive electrode wire guide channel 141. Although the first shape is irregular, this special geometric pattern can form uniformly distributed electric field lines with the negative electrode, and the electric field lines are perpendicular to the surface of the gel-transfer film. Moreover, compared with the traditional positive electrode wire used for forming the positive electrode by multiple round trips and parallel winding, this first shape is shorter, which can save the amount of precious metals.
[0054] Specifically, the positive supporting piece 21 also includes a limiting member 214, which is located at one end of the vertical piece 212. The limiting member 214 is used to prevent the positive supporting piece 21 from shaking in the length direction after being installed on the first side wall 121. Specifically, the limiting member 214 is installed at the upper end of the vertical piece 222. The limiting member 214 includes a transverse piece 2141 and two vertical pieces 2142. One end of the two vertical pieces 2142 is connected to the two ends of the transverse piece 2141. The length of the transverse piece 2141 itself is the same as the length of the installation groove 1211 at the top of the first side wall 121. Therefore, after the positive supporting piece 21 is installed on the first side wall 121, the position can be fixed to prevent the positive supporting piece 21 from moving in the length direction of the first side wall 121 (i.e. Figure 3 As shown, the positive carrier sheet 21 is prevented from moving left and right).
[0055] Specifically, the length of the vertical piece 212 is the same as the distance between the top and bottom mounting grooves 1211 on the first side wall 121. The positive supporting piece 21 is made of an elastic material with a certain elasticity. During installation, the elasticity of the positive supporting piece 21 is utilized to insert the two ends of the positive supporting piece 21 into the top and bottom mounting grooves 1211 on the first side wall 121, respectively, so that the positive supporting piece 21 is fixed in the height direction of the first side wall 121 (i.e. Figure 3 As shown, the positive supporting piece 21 will not move up and down after being installed in the installation groove 1211).
[0056] Specifically, when the first holes 211 are not arranged in an array on the positive carrier sheet 21, the design and threading requirements for the first holes 211 are as follows: when the positive electrode wire passes through the first hole 211 on the side of the positive carrier sheet 21 facing away from the negative carrier sheet 31, the positive electrode wire can pass through the adjacent first hole 211 back to the side of the positive carrier sheet 21 facing the negative carrier sheet 31, or the positive electrode wire runs toward the outside of the positive carrier sheet 21, thereby not being blocked by the positive carrier sheet 21. Specifically, the placement requirements for the first holes 211 include at least the following: when the first holes 211 are located at the edge of the positive carrier sheet 21 and the positive electrode wire runs toward the outside of the positive carrier sheet 21, only one first hole 211 can be provided at that location. Specifically, the distance between the first hole 211 and the edge of the positive carrier sheet 21 is less than or equal to 1 mm. For example, when the first hole 211 is located at the end of the transverse sheet 213, the distance between the first hole 211 and the edge of the transverse sheet 213 is less than or equal to 1 mm. When the first hole 211 is located within the positive carrier sheet 21 or at the edge of the positive carrier sheet 21, but the positive electrode wire does not run toward the outside of the positive carrier sheet 21, at least two first holes 211 should be provided consecutively at that location, and the distance between the two first holes 211 should be less than or equal to 1 mm. For example, when the first holes 211 are located at both ends of the vertical sheet 212, two adjacent first holes 211 should be provided on the vertical sheet, and the distance between the two adjacent first holes 211 should be less than or equal to 1 mm. The setting of the first hole 211 allows the positive electrode wire to quickly return to the side of the positive carrier sheet 21 facing the negative carrier sheet 31 through the adjacent first hole 211 or not be blocked by the positive carrier sheet 21 when passing through the first hole 211 to the side of the positive carrier sheet 21 facing away from the negative carrier sheet 31, so that when the positive electrode wire changes its direction, only about 1 mm of the positive electrode wire is located on the side of the positive carrier sheet 21 facing away from the negative carrier sheet 31. Therefore, basically all electrode wires immersed in the electrophoretic fluid can participate in the formation of a uniform electric field, greatly improving the efficiency and uniformity of protein transfer, and reducing the previous problem that the wiring is wrapped around the outside of the frame, so that the electric field generated by this part exceeds the range of the transfer medium and cannot be used for protein transfer, resulting in waste.
[0057] In some embodiments, the positive electrode wire is a platinum wire.
[0058] The negative electrode assembly 30 includes: a negative carrier sheet 31 and a negative electrode wire 32. The negative carrier sheet 31 is detachably mounted on the second side wall 122 of the second pair of inner side walls 12. A plurality of second holes 311 are provided on the negative carrier sheet 31. One end of the negative electrode wire 32 is fixed on the negative carrier sheet 31, and the other end passes through the plurality of second holes 311 in sequence to form a negative electrode having a second shape on the surface of the negative carrier sheet 31 facing away from the second side wall 122.
[0059] The anode assembly is manufactured as a single module, allowing for individual installation and replacement within the frame. This modular design not only facilitates disassembly and recycling, but also enables the rapid extraction and reuse of the metal from the anode assembly, meeting the requirements of green chemistry and sustainable laboratory development. This reduces disposable material waste, extends equipment life, and reduces the environmental burden of laboratory operations.
[0060] The positive electrode wire is routed on the positive carrier sheet to form a positive electrode with a first shape, and the negative electrode wire is routed on the negative carrier sheet to form a negative electrode with a second shape, thereby arranging the electrode wire only in the effective area inside the protein transfer core, eliminating the waste of electricity in the "dead zone" of the non-working area caused by routing the wire in the past.
[0061] In some embodiments, the negative supporting sheet 31 is inserted into the mounting groove 1221. Specifically, both ends of the negative supporting sheet 31 are inserted into the top groove and the bottom groove of the mounting groove 1221 respectively.
[0062] In some embodiments, the structure of the negative electrode assembly 30 is the same as that of the positive electrode assembly 20. Figure 5 、 Figure 6 The second hole is evenly distributed on the negative carrier sheet in an a×b array. The second shape is a rectangular pulse or a rectangular vortex. Specifically, the structure of the negative electrode assembly 30 refers to the above-mentioned positive electrode assembly 20. Figure 5 、 Figure 6 The description of the structure shown will not be repeated here.
[0063] Specifically, the a×b array of the second holes may be the same as the m×n array of the first holes, or the a×b array of the second holes may be different from the m×n array of the first holes.
[0064] In some embodiments, the first shape and the second shape are the same.
[0065] In some embodiments, the first shape and the second shape are different, for example, the first shape is Figure 7 The special geometric pattern shown, the second shape is Figure 5 The rectangular pulse shape shown.
[0066] Of course, the distribution of the second holes 311 in the load sheet 31 in the negative electrode assembly 30 may be other distributions.
[0067] For example, in some embodiments, the negative carrier sheet 31 has multiple sets of parallel guide structures, each set of guide structures comprising a plurality of second holes 311 arranged in a linear pattern. The negative electrode wire 32 passes through each of the second holes 311 in the guide structures at the bottom, one by one, and then winds around to the top of the negative carrier sheet 31, forming a second shape in a rectangular pulse-like winding path. The second shape is a rectangular pulse.
[0068] For the case where the second holes 311 are not arranged in an array on the negative carrier sheet 31, the design and threading requirements of the second holes 311 are as follows: when the negative electrode wire passes through the second hole 311 and is located on the side of the negative carrier sheet 31 facing away from the positive carrier sheet 21, the negative electrode wire 32 can pass through the adjacent second hole 311 and return to the side of the negative carrier sheet 31 facing the positive carrier sheet 21, or the direction of the negative electrode wire 32 is located at the position of the adjacent second hole 311 and is not blocked by the negative carrier sheet 31, and then pass through the next adjacent second hole 311 and return to the side of the negative carrier sheet 31 facing the positive carrier sheet 21.
[0069] Figure 8 A distribution diagram of the second pores in the negative electrode assembly in another embodiment of the present disclosure is shown.
[0070] In some embodiments, as Figure 8 As shown, each guiding structure includes two circular holes 3111 and one long hole 3112, the two circular holes 3111 and one long hole 3112 are arranged in a line, and the two circular holes 3111 are located at both ends of the long hole 3112, and the distance between the two circular holes 3111 and the long hole 3112 is less than or equal to 1 mm. Therefore, in the path of the negative electrode wire, after the negative electrode wire passes through a circular hole 3111 of the guide structure to the side of the negative carrier sheet 31 facing away from the positive carrier sheet 21, the direction of the negative electrode wire 32 makes the negative electrode wire 32 located at the position of the long hole 3112 and not blocked by the negative carrier sheet 31, and then passes through another circular hole 3111 of the guide structure to the side of the negative carrier sheet 31 facing the positive carrier sheet 21, and then passes through a circular hole 3111 of the next adjacent guide structure to the side of the negative carrier sheet 31 facing away from the positive carrier sheet 21, and follows a rectangular pulse winding path until it reaches the top of the negative carrier sheet 31, and then is connected to the electrode connector 40 for connecting the negative electrode through the negative electrode wire guide channel 142. The design of the guide structure ensures that when the negative electrode wire 32 passes between each second hole 311, only about 1-2 mm of the negative electrode wire is located on the side of the negative carrier sheet 31 facing away from the positive carrier sheet 21. Therefore, basically all electrode wires immersed in the electrophoretic fluid can participate in the formation of a uniform electric field, greatly improving the efficiency and uniformity of protein transfer, and reducing the previous problem of the wire being routed around the outside of the frame, causing the electric field generated by this part to exceed the range of the transfer medium and be unable to be used for protein transfer, resulting in waste.
[0071] Figure 9 A distribution diagram of the second pores in the negative electrode assembly in another embodiment of the present disclosure is shown.
[0072] In some embodiments, as Figure 9 As shown, each guide structure includes two circular holes 3111 and two elongated holes 3112. The two elongated holes 3112 are arranged adjacent to each other, and the two circular holes 3111 are located at the other ends of the two elongated holes 3112. When the negative electrode wire 32 is routed, the negative electrode wire 32 passes through the circular hole 3111 at one end of the guide structure, reaches the side of the negative carrier sheet 31 facing away from the positive carrier sheet 21, then passes through the adjacent elongated hole 3112 to the side of the negative carrier sheet 31 facing the positive carrier sheet 21, passes through another elongated hole 3112, and then exits through the circular hole 3111 at the other end. The guide structure is designed so that within a guide structure, the negative electrode wire 32 is substantially entirely located on the side of the negative carrier sheet 31 facing the positive carrier sheet 21, and the length of the negative electrode wire 32 located on the side of the negative carrier sheet 31 facing away from the positive carrier sheet 21 is less than or equal to 2 mm.
[0073] Specifically, the distances between adjacent guide structures are the same, and the width of each pulse in the rectangular pulse shape in the formed second shape is the same.
[0074] It is understood that the second hole in the negative electrode assembly 30 is Figure 8 、 Figure 9 The distribution structure in FIG. 1 is also applicable to the positive electrode assembly 20 .
[0075] Specifically, the height of the negative carrier sheet 31 is the same as the distance between the top and bottom mounting grooves 1221 on the second side wall 122, and the length of the negative carrier sheet 31 is the same as the length of the top and bottom mounting grooves 1221 on the second side wall 122. The negative carrier sheet 31 is made of an elastic material with a certain elasticity. During installation, the elasticity of the negative carrier sheet 31 allows the two ends of the negative carrier sheet 31 to be inserted into the top and bottom mounting grooves 1221 on the second side wall 122, respectively, so that the negative carrier sheet 31 is fixed in the mounting grooves 1221 (i.e., Figure 4 As shown, the negative supporting plate 31 will not move up, down, left, or right after being installed in the installation groove 1211).
[0076] Specifically, the negative carrier sheet 31 may be a rectangular sheet, on which the guide structure is formed.
[0077] In some embodiments, the negative electrode wire is stainless steel. According to electrochemical principles, during the transfer process, only the positive electrode (or active electrode, depending on the system configuration) undergoes a metal redox reaction at the interface with the electrophoretic fluid, which poses a risk of rapid corrosion. The chemical reaction at the negative electrode, however, does not involve the metal itself, and therefore places less stringent material requirements. Using stainless steel wire does not affect instrument performance and can save 50% of precious metals, significantly reducing production costs and material input. Of course, the negative electrode wire can also be made of other conductive, non-precious metals.
[0078] It is understood that the negative carrier sheet 31 and the negative electrode wire 32 in the negative electrode assembly 30 may also be integrally formed. For example, a single piece of conductive metal such as graphite or stainless steel may be used as the negative electrode. This is sufficient as long as a uniform electric field is generated between the positive and negative electrodes, with the electric field lines being perpendicular to the surface of the gel-transfer film.
[0079] It is understood that the removable installation of the positive and negative load plates 21 and 31 is not limited to being fixed by insertion into the mounting slots, and other structures for achieving the removable installation of the positive and negative load plates 21 and 31 may also be employed. For example, contacts may be provided on the second pair of inner sidewalls 12 of the frame 10, and mounting holes may be provided on the positive and negative load plates 21 and 31 that correspond to and match the contacts, with installation or removal being accomplished through the engagement of the mounting holes on the positive and negative load plates 21 and 31 with the corresponding protrusions on the contacts.
[0080] A pair of electrode connectors 40 are mounted on top of the frame 10. The other end of the positive electrode wire 22 is fixedly connected to the electrode connector 40 for connecting to the positive electrode, and the other end of the negative electrode wire 32 is fixedly connected to the electrode connector 40 for connecting to the negative electrode. Specifically, after the positive electrode wire 22 is formed into a first shape on the positive carrier sheet 21, the free end of the positive electrode wire 22 passes through the positive electrode wire guide channel 141 and is then connected to the electrode connector for connecting to the positive electrode via standard hardware such as nuts and washers. After the negative electrode wire 32 is formed into a second shape on the negative carrier sheet 31, the free end of the negative electrode wire 32 passes through the negative electrode wire guide channel 142 and is then connected to the electrode connector 40 for connecting to the negative electrode via standard hardware such as nuts and washers.
[0081] In some embodiments, the electrode head 40 is disposed on top of the first pair of inner sidewalls 11. Specifically, the electrode connector 40 is disposed near the first sidewall 121 of the second pair of inner sidewalls 12, which can reduce the length of the positive electrode wire guide channel, thereby saving the length of the positive electrode precious metal wire and reducing the amount of precious metal used.
[0082] In some embodiments, the protein transfer core also includes an anti-foolproof component for preventing the electrode connector 40 from being connected to the positive and negative poles on the electrophoresis tank cover when the electrophoresis tank cover is installed. The anti-foolproof component satisfies that if the positive electrode connector 40 corresponds to the negative pole of the electrophoresis tank cover, the electrophoresis tank cover cannot be installed on the electrophoresis tank. For example, the two electrode connectors 40 are designed with different structures, and the corresponding structures for connecting the positive electrode connector and the structures for connecting the negative electrode connector on the electrophoresis tank cover are matched with them respectively. In this way, when the polarity of the electrode connector 40 does not match the polarity on the electrophoresis tank cover, the electrophoresis tank cover and the electrode connector 40 cannot be connected, thereby avoiding the reverse polarity of the two electrode connectors 40. The risk of experimental failure or equipment damage caused by wrong polarity connection is avoided, and the reliability and safety of laboratory operations are improved.
[0083] Figure 10A structural diagram of a joint cover in an embodiment of the present disclosure is shown. Figure 11 A structural diagram of the connector cover at another angle in an embodiment of the present disclosure is shown.
[0084] like Figure 10 、 Figure 11 As shown, in some embodiments, the protein transfer core further includes two connector covers 50, both of which are provided with through holes 51. The two connector covers 50 are respectively sleeved on the two electrode connectors 40 through the through holes 51 and fixed on the top of the first pair of inner side walls 11. Both connector covers 50 are provided with markings. For example, the connector cover installed on the positive electrode connector is provided with a "red" character, and the connector cover installed on the negative electrode connector is provided with a "black" character, so that the installer can quickly know which electrode connector is the positive electrode connector and which electrode connector is the negative electrode connector. The risk of experimental failure or equipment damage caused by wrong polarity connection is reduced, and the reliability and safety of laboratory operations are improved.
[0085] like Figure 11 As shown, in some embodiments, the connector cover 50 is provided with a first engaging member 52 having a hook. The upper portion of the first pair of inner side walls 11 is provided with a second engaging member 111 that cooperates with the first engaging member 52. The two connector covers 50 are respectively mounted on the two electrode connectors 40 through the through holes 51 and are then fixed to the top of the first pair of inner side walls 11 through the cooperation between the first engaging member 52 and the corresponding second engaging member 111. The provision of the connector cover 50 makes it easy to remove and replace the connector cover 50, reducing maintenance costs.
[0086] Specifically, the second engaging member 111 is a through hole. The second engaging member 111 can cooperate with the first engaging member 52 to fix the joint cover 50 on the top of the first pair of inner side walls 11, and can also serve as a liquid through port of the frame, so that during the transfer process, the heated transfer buffer solution flows out of the frame 10 from the liquid through port, forming convection with the peripheral transfer buffer solution, thereby reducing the temperature of the transfer buffer solution in the frame 10.
[0087] In some embodiments, the protein transfer core further includes a temperature sensing element (not shown in the figure), which is connected to a pair of electrode connectors 40. The temperature sensing element is used to realize real-time temperature control monitoring within the frame 10. The specific location of the temperature sensing element does not affect the electric field between the positive electrode and the negative electrode, and can be located within the frame 10, and is not limited here. For example, the temperature sensing element can be set on the side of the positive carrier sheet 21 or the negative carrier sheet 31 facing the second pair of inner side walls 12. Furthermore, the temperature sensing element is connected to a cooling system, and the cooling system can regulate the temperature according to the temperature of the temperature sensing element.
[0088] This embodiment also provides a protein transfer system, which includes the above-mentioned protein transfer core.
[0089] In some embodiments, the protein transfer system comprises: an electrophoresis tank, a transfer pad, a transfer sandwich clip, a blue ice box, and the above-mentioned protein transfer core.
[0090] The text and drawings in this disclosure are provided as examples only to help understand the present disclosure. They should not be interpreted as limiting the scope of the present disclosure in any way. Although certain embodiments and examples have been provided, it will be clear to those skilled in the art based on what is disclosed herein that the embodiments and examples shown may be modified without departing from the scope of the present disclosure.
[0091] Although the present disclosure has been described with exemplary embodiments, various changes and modifications may be suggested to one skilled in the art. It is intended that the present disclosure encompass such changes and modifications as fall within the scope of the appended claims.
[0092] Nothing in this disclosure should be construed as implying that any particular element, step, or function is an essential element that must be included in the claims scope. The scope of the patented subject matter is defined solely by the claims.
Claims
1. A protein transfer core, characterized in that include: The frame includes at least two pairs of oppositely disposed inner side walls, a first pair of inner side walls having a bearing groove for mounting a rotary mold clamp, and each side wall of the second pair of inner side walls being perpendicular to each side wall of the first pair of inner side walls; A positive electrode assembly, comprising: a positive carrier sheet and a positive electrode wire, wherein the positive carrier sheet is detachably mounted on a first sidewall of the second pair of inner sidewalls, the positive carrier sheet being provided with a plurality of first holes, one end of the positive electrode wire being fixed to the positive carrier sheet, and the other end of the positive electrode wire being sequentially passed through the plurality of first holes to form a positive electrode having a first shape on a surface of the positive carrier sheet facing away from the first sidewall; A negative electrode assembly comprising: a negative carrier sheet and a negative electrode wire, wherein the negative carrier sheet is detachably mounted on the second sidewall of the second pair of inner sidewalls, the negative carrier sheet being provided with a plurality of second holes, one end of the negative electrode wire being fixed to the negative carrier sheet, and the other end of the negative electrode wire being sequentially passed through the plurality of second holes to form a negative electrode having a second shape on a surface of the negative carrier sheet facing away from the second sidewall; and A pair of electrode connectors are installed on the top of the frame, the other end of the positive electrode wire is fixedly connected to one of the electrode connectors, and the negative electrode wire is fixedly connected to the other electrode connector.
2. The protein transfer core according to claim 1, characterized in that The first holes are evenly distributed on the positive carrier sheet in an m×n array, and the second holes are evenly distributed on the negative carrier sheet in an a×b array.
3. The protein transfer core according to claim 1 or 2, characterized in that The first shape is a rectangular pulse shape or a rectangular vortex shape, and the second shape is a rectangular pulse shape or a rectangular vortex shape.
4. The protein transfer core according to claim 1, characterized in that The positive supporting plate includes a vertical plate and a plurality of horizontal plates arranged in parallel and at intervals on the vertical plate. The first holes are provided at both ends of the horizontal plate, and a pair of first holes are provided at both ends of the vertical plate. The path of the positive electrode wire is: one end is fixed at the first hole on the top side of the vertical plate, and it starts to pass through the first hole on the same side of each horizontal plate in turn, and then changes direction through a pair of first holes on the bottom side of the vertical plate, and passes through the first hole on the other side of each horizontal plate in turn.
5. The protein transfer core according to claim 1, characterized in that The negative carrier sheet has multiple groups of parallel guide structures, each group of guide structures is composed of multiple second holes arranged in a linear shape. The negative electrode wire passes through each second hole in the guide structure located at the bottom one by one until it reaches the top of the negative carrier sheet, forming the second shape according to a rectangular pulse-shaped winding path.
6. The protein transfer core according to claim 1, characterized in that The second pair of inner side walls of the frame are each provided with mounting grooves, and the positive bearing piece and the negative bearing piece are respectively inserted into the corresponding mounting grooves.
7. The protein transfer core according to claim 1, characterized in that The top and bottom of each inner side wall of the second pair of inner side walls are provided with the mounting groove.
8. The protein transfer core according to claim 1, characterized in that The two electrode heads are respectively arranged on the top of each side wall of the first pair of inner side walls. Two guide channels are provided on the frame, and the two guide channels respectively extend to one of the electrode heads, and each guide channel is for one of the positive electrode wire and the negative electrode wire to pass through.
9. The protein transfer core according to claim 1, characterized in that The positive electrode wire is a platinum wire, and the negative electrode wire is a stainless steel wire.
10. The protein transfer core according to claim 1, characterized in that It also includes: two joint covers, which are respectively sleeved on the corresponding electrode joints and fixed to the frame, and the joint covers are provided with marks.
11. The protein transfer core according to claim 1, characterized in that It also includes a temperature sensing element, which is connected to the pair of electrode connectors.
12. A protein transfer system, characterized in that: The invention comprises the protein transfer core according to any one of claims 1 to 10.